CN115627215A - Efficiency of degradation is improved microbial fermentation jar - Google Patents

Efficiency of degradation is improved microbial fermentation jar Download PDF

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CN115627215A
CN115627215A CN202211569845.3A CN202211569845A CN115627215A CN 115627215 A CN115627215 A CN 115627215A CN 202211569845 A CN202211569845 A CN 202211569845A CN 115627215 A CN115627215 A CN 115627215A
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fermentation reactor
gas injection
injection unit
rate
fermentation
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CN115627215B (en
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范现国
马琪
胡长利
宋蕾
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Beijing Zaiyi Biotechnology Co ltd
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    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

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Abstract

The invention relates to the field of fermentation, and relates to a microbial fermentation tank for improving degradation efficiency, which comprises a head-end fermentation reactor, a first gas injection unit and a second gas injection unit, wherein the head-end fermentation reactor is provided with a barrel-shaped first tank body, and the bottom in the first tank body is provided with the first gas injection unit for providing gas required in the pre-fermentation process; the tail end fermentation reactor is provided with a second tank body used for fermenting the pre-fermented product generated by the head end fermentation reactor again, a second gas injection unit used for fermenting the pre-fermented product again is arranged at the bottom of the second tank body, and a second heating unit is arranged outside the second tank body; an air compressor provided with a sterilization mechanism for sterilizing the injected gas; and the controller is used for injecting the pre-fermentation liquid into the tail end fermentation reactor when the growth vigor of the microorganisms in the head end fermentation reactor is in the logarithmic growth phase, and adjusting the temperature conversion frequency of the heating mechanism according to the growth rate of the microorganisms in the tail end fermentation reactor so as to improve the degradation efficiency of the microorganisms in the culture solution.

Description

Efficiency of degradation is improved microbial fermentation jar
Technical Field
The invention relates to the field of fermentation, in particular to a microbial fermentation tank capable of improving degradation efficiency.
Background
Microbial fermentation refers to a process of converting raw materials into products required by human beings through a specific metabolic pathway by using microorganisms under appropriate conditions. The level of microbial fermentation production depends mainly on the genetic characteristics of the strain itself and the culture conditions. The microbial fermentation process is divided into aerobic fermentation and anaerobic fermentation according to the requirement of fermentation conditions. Among them, the aerobic fermentation method includes liquid surface culture fermentation, fermentation on the surface of a porous or granular solid medium, and aerobic submerged fermentation. The factors influencing the aerobic fermentation are mainly the temperature and the oxygen content suitable for the growth of microorganisms and the uniform transfer of oxygen.
Chinese patent CN 113213992B discloses a microbial fermentation reaction kettle, which comprises a reaction kettle body and a reaction kettle cover mounted on the reaction kettle body, wherein a driving device is mounted on the reaction kettle cover, and an inlet and outlet device is arranged on the reaction kettle body to improve the stirring efficiency of raw materials, and simultaneously, the raw materials can be fully stirred in the reaction kettle, but the technical problem of how to control the fermentation temperature and oxygen injection condition in the aerobic fermentation process to improve the fermentation efficiency is still not solved.
Disclosure of Invention
Therefore, the invention provides a microbial fermentation tank capable of improving degradation efficiency, and can solve the technical problem that the microbial fermentation efficiency in the microbial fermentation tank cannot be improved by using the residual heat in the use process of an air compressor.
To achieve the above objects, the present invention provides a microbial fermentation tank with improved degradation efficiency, comprising:
the head end fermentation reactor is provided with a barrel-shaped first tank body, the first tank body is provided with a space for fermentation of microorganisms and culture solution wrapped in a membrane, a first gas injection unit for providing gas required in the pre-fermentation process is arranged at the bottom in the first tank body, and a first heating unit is arranged outside the first tank body;
the tail end fermentation reactor is connected with the head end fermentation reactor and is provided with a second tank body used for fermenting the pre-fermented product generated by the head end fermentation reactor again, a second gas injection unit used for fermenting the pre-fermented product again is arranged at the bottom of the second tank body, and a second heating unit is arranged outside the second tank body;
the air compressor is provided with two exhaust pipelines, each exhaust pipeline injects gas into the head-end fermentation reactor and the tail-end fermentation reactor respectively, a sterilization mechanism for sterilizing the injected gas is arranged in the air compressor, heating mechanisms for providing convection for the air in the air compressor are arranged on two sides of the air compressor, and the heating mechanisms are connected with the first heating unit and the second heating unit so as to transfer the heat energy of the heating mechanisms to the head-end fermentation reactor and the tail-end fermentation reactor;
and the controller is connected with the head-end fermentation reactor, the tail-end fermentation reactor and the air compressor and is used for injecting the pre-fermentation liquid into the tail-end fermentation reactor when the growth vigor of microorganisms in the head-end fermentation reactor is in a logarithmic growth phase, and adjusting the temperature conversion frequency of the heating mechanism according to the growth rate of the microorganisms in the tail-end fermentation reactor so as to improve the degradation efficiency of the microorganisms in the culture solution.
Further, the controller compares the cell density of the microorganisms in the first-end fermentation reactor obtained in the preset culture time with the cell density of a microorganism growth standard curve to judge the growth rate of the microorganisms, and when the growth rate does not meet the standard, the controller judges whether to adjust the rotation rate of the first gas injection unit and the gas injection amount according to the temperature of the first-end fermentation reactor or the dispersion degree of the microorganisms, wherein the controller obtains the cell density change rate P of the microorganisms in the first-end fermentation reactor in the preset culture time and compares the cell density change rate P of the microorganisms in the logarithmic growth phase of the microorganism growth standard curve to judge the growth rate of the microorganisms, wherein,
when P is less than P-delta P, the control machine judges that the growth rate of the microorganisms in the first-end fermentation reactor does not meet the standard, and the growth cycle of the microorganisms is not in the logarithmic growth phase;
when the P-delta P is not less than the P + delta P, the control machine judges that the growth rate of the microorganisms in the first-end fermentation reactor meets the standard, and the growth cycle of the microorganisms is in the logarithmic growth phase;
when P is more than P + delta P, the control machine judges that the growth rate of the microorganisms in the first-end fermentation reactor does not meet the standard;
wherein, the delta p is the cell density change rate error value preset by the controller.
Further, under a first condition, the control machine increases the first temperature of the first heat exchanger of the heating mechanism according to the temperature conversion frequency F of the first heat exchanger and the second heat exchanger of the heating mechanism to increase the temperature of the head-end fermentation reactor, wherein when F is less than or equal to F, the control machine increases the first temperature w1 to w11 of the first heat exchanger by using a first adjusting parameter k1, and w11= w1 × k1 is set; when F is larger than F, the control machine opens a reversing valve connected between the first heating unit and the second heating unit to transfer the heat of the second heating unit to the first heating unit;
the first condition is that the fermentation temperature of the first-end fermentation reactor is lower than a preset pre-fermentation temperature, the growth cycle of microorganisms in the first-end fermentation reactor is not in a logarithmic growth phase, and the control machine presets a temperature conversion frequency F, wherein k1= k0 x (1 + (F-F)/F0), k0 is a preset regulation parameter standard value, and F0 is a preset temperature conversion frequency standard value.
Further, the controller adjusts the rotation rate of the first gas injection unit and the gas injection rate according to the microorganism dispersion degree Y in the head-end fermentation reactor under a second condition, wherein when Y is less than or equal to Y, the controller increases the gas injection rate vs to vs1 of the first gas injection unit, and sets vs1= vs x (1 + (Y-Y)/Y); when Y is larger than Y, the control machine increases the rotation speed cs of the first gas injection unit to cs1, and sets cs1= cs x (1 + (Y-Y)/Y);
and under the second condition that the fermentation temperature of the first-end fermentation reactor is higher than the preset pre-fermentation temperature and the growth cycle of microorganisms in the first-end fermentation reactor is not in the logarithmic phase, the dispersion degree standard value Y is preset by the controller.
Further, the fermentation reactor at the head end comprises a microorganism injection unit, the microorganism injection unit comprises a driver for controlling the rotation speed of the injector, the controller presets a first gas injection unit rotation speed maximum value csmax, the adjusted rotation speed cs1 of the first gas injection unit is greater than the preset first gas injection unit rotation speed maximum value csmax, the controller judges that the rotation speed of the first gas injection unit is set to csmax, meanwhile, the rotation speed vb during the next microorganism injection is increased to vb1, and vb1= vb x (1 + (cs 1-csmax)/csmax) is set.
Further, after the pre-fermented matter of the head-end fermentation reactor is injected into the tail-end fermentation reactor, the microbial growth rate of the tail-end fermentation reactor is detected after a preset re-fermentation time, the acquired microbial growth rate H of the tail-end fermentation reactor is compared with a preset growth rate H by the control machine, if the microbial growth rate of the tail-end fermentation reactor is greater than the preset growth rate, the control machine does not adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit, and if the microbial growth rate of the tail-end fermentation reactor is less than the preset growth rate, the control machine judges whether to adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit according to the temperature of the tail-end fermentation reactor.
Further, when the growth rate of the microorganisms in the tail end fermentation reactor is less than the preset growth rate, the controller obtains the temperature D of the tail end fermentation reactor to compare with the preset re-fermentation temperature D, and judges whether to adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit, wherein,
when D is less than or equal to D, the control machine determines to increase the first temperature w2 of the second heat exchanger to w21, setting w21= w2 × (1 + (D-D)/D);
when D > D, the control machine increases the rotation rate cw to cw1 of the second gas injection unit, sets cw1= cw x (1 + (D-D)/D), and increases the gas injection rate vw to vw1 of the second gas injection unit, sets vw1= vwx (1 + (D-D)/D).
Further, at least one ultrasonic generator is arranged on the outer wall of the tail end fermentation reactor and used for promoting microbial fermentation, the control machine presets a second gas injection unit rotation rate cwmax, when the adjusted rotation rate cw1 of the second gas injection unit is greater than a preset second gas injection unit rotation rate maximum value cwmax, the control machine sets the rotation rate of the second gas injection unit to cwmax and starts the ultrasonic generator, the frequency E of the ultrasonic generator is adjusted to E1, and E1= E × (1 + (cw 1-cwmax)/cwmax) is set.
Further, the control machine shortens the temperature change frequency f0 to f1 of the air compressor according to the adjusted injection rate vs1 of the first gas injection unit and the adjusted injection rate vw1 of the second gas injection unit, and sets f1= fx (1-0.5 × (vs 1-vs)/vs × 0.8 × (vw 1-vw)/vw).
Further, the controller increases the temperature conversion frequency fq of the air compressor to fq1 under a third condition, and sets fq1= fq × 1.25, the third condition is that P > P + Δp, and the controller determines that the air compressor sterilization does not meet the standard, and q =0,1.
Compared with the prior art, the invention has the advantages that the microorganisms are attached to the membrane, the microorganisms attached to attachments can be gathered and grown in the culture solution and are separated from the culture solution, the microorganisms on the attachments are propagated by using nutrient substances of the culture solution, and are separated from the attachments gradually in the growth and propagation process so that the microorganisms grow orderly, the microorganisms on the attachments are ensured to be remained for recycling, the using time of the microorganisms can be prolonged while fermentation is realized, meanwhile, the invention is provided with a head-end fermentation reactor for culturing the microorganisms to a logarithmic phase and a tail-end fermentation reactor for stably culturing fermentation products of the logarithmic phase, aerobic fermentation is realized by injecting sterilized gas into the head-end fermentation reactor and the tail-end fermentation reactor, the fermentation liquid in each reactor is stirred, and the optimal temperature suitable for the fermentation process is provided for the reactors by using the heat of a heating mechanism for promoting the gas convection in an air compressor, so that the degradation efficiency of the microorganisms in the fermentation liquid is improved.
Particularly, the invention evaluates the growth rate of the microorganism in the head-end fermentation reactor according to the comparison of the cell density change rate of the microorganism in the head-end fermentation reactor with the cell density change rate of the growth logarithmic phase in a standard curve, and simultaneously judges whether the microorganism is in the growth logarithmic phase, wherein if the cell density change rate of the microorganism is less than the error range of the cell density change rate of the growth logarithmic phase of the standard curve, the growth rate of the microorganism in the head-end fermentation reactor is not in accordance with the standard, and if the cell density change rate of the microorganism is lower than the standard value, the growth rate of the microorganism is proved to be not reach the logarithmic phase, the efficiency of the current head-end fermentation reactor is too low, if the cell density change amount of the microorganism is greater than the error range of the cell density change rate of the growth logarithmic phase of the standard curve, the current growth rate is too high, the condition of introducing mixed bacteria is characterized, the microorganism is detected, and if the cell density change rate of the microorganism is in the error range of the cell density change rate of the logarithmic phase of the growth phase of the standard curve, the microorganism in the head-end fermentation reactor is proved to be in accordance with the standard, and the growth rate of the microorganism in the growth cycle is in the pre-growth logarithmic phase, and the fermentation reactor is injected into the fermentation broth.
Particularly, under the condition that the growth cycle of microorganisms in the first-end fermentation reactor does not reach the growth logarithmic phase within the preset time and the temperature of the first-end fermentation reactor is detected to be too low, the problem that the temperature of the first-end fermentation reactor is too low and the growth rate of the microorganisms cultured by the first-end fermentation reactor is too low due to the fact that the temperature conversion frequency is too high is solved by correcting the high-temperature of the first heat exchanger according to the temperature conversion frequency of the first heat exchanger and the temperature conversion frequency of the second heat exchanger, wherein the control machine obtains the temperature conversion frequency which is lower than the preset temperature conversion frequency, and the reason that the temperature of the second heating unit is too low is not short in temperature retention time due to the temperature conversion frequency and is too low in the setting temperature of the first heat exchanger, so that the control machine selects the maximum adjusting parameters to increase the high-temperature of the first heat exchanger, the temperature of the first-end fermentation reactor is further improved, when the temperature conversion frequency of the air compressor is obtained to exceed the preset temperature conversion frequency, the reason that the fermentation reactor fermentation head-end fermentation temperature of the first-end fermentation reactor cannot reach the standard at present time is that the first-end fermentation reactor is too high temperature conversion frequency, the first heating time of the first heating unit is shortened, the first heating unit, the heating unit is shortened, and the second heating unit is started by the control valve, and the second heating unit is started to be connected between the second heating unit, and the heating unit is prolonged, and the first heating unit.
Particularly, the fermentation temperature in the first-end fermentation reactor is determined to be higher than the preset pre-fermentation temperature, which indicates that the reason that the growth period of microorganisms in the first-end fermentation reactor does not reach the logarithmic growth phase within the preset time is not the fermentation temperature, but the reason that the microorganisms in the first-end fermentation reactor are not uniformly mixed with the culture solution or the gas injection amount is insufficient is that the dispersion degree of the microorganisms in the first-end fermentation reactor is not uniform or the gas injection amount is insufficient.
Particularly, the microorganism injection unit capable of controlling the injection rotating speed is arranged at the top of the head-end fermentation reactor, when the rotating speed of the first gas injection unit reaches the maximum, in order to avoid the microbial cell rupture caused by the overlarge rotating speed of the first gas injection unit, the control machine adjusts the rotating speed of the first gas injection unit to the preset maximum value, and controls and improves the rotating speed of the microorganism injection unit, so that the initial dispersion degree of microorganisms in the next pre-fermentation process is improved.
In particular, the present invention detects the microorganism growth rate of the tail-end fermentation reactor for re-fermentation, compares the obtained microorganism growth rate of the tail-end fermentation reactor with a preset growth rate, and determines whether the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit are adjusted according to the temperature of the tail-end fermentation reactor, wherein if the microorganism growth rate of the tail-end fermentation reactor is greater than the preset growth rate, it is determined that the current microorganism growth rate of the tail-end fermentation reactor meets the standard, and if the microorganism growth rate of the tail-end fermentation reactor is less than the preset growth rate, it is determined that the current microorganism growth rate of the tail-end fermentation reactor does not meet the standard, the controller compares the temperature of the tail-end fermentation reactor with the preset re-fermentation temperature to determine the reason that the microorganism growth rate of the tail-end fermentation reactor does not meet the standard, if the temperature of the tail-end fermentation reactor is less than the preset growth rate, the high-temperature of the second heat exchanger is increased to increase the heating temperature of the second heating unit and further increase the re-fermentation temperature of the tail-end fermentation reactor, and if the temperature of the tail-end fermentation reactor is less than the preset growth rate, it is determined that the rotation rate of the gas injection rate of the second gas injection reactor is sufficient for the re-fermentation reactor, thereby increasing the propagation rate of the re-fermentation reactor.
Particularly, the invention sets a plurality of ultrasonic generators on the tail end fermentation reactor, the ultrasonic generators have the functions of oscillating and strengthening culture on microorganisms in the tail end fermentation reactor, the controller obtains that the rotation speed of the second gas injection unit exceeds the preset maximum value of the rotation speed of the second gas injection unit, and the controller increases the frequency of the ultrasonic generators according to the difference value of the regulated rotation speed and the preset rotation speed of the second gas injection unit by starting the ultrasonic generators so as to promote the fermentation of the microorganisms in the tail end fermentation reactor.
Particularly, in order to prevent the quantity of gas sterilized in the air compressor from not reaching the injection quantity into each fermentation reactor, the controller adjusts the temperature conversion frequency of the air compressor according to the obtained and adjusted gas injection rate of the first gas injection unit and the second gas injection rate of the second gas injection unit so as to improve the convection rate in the air compressor, improve the gas sterilization rate in the air compressor and ensure that a large amount of gas qualified for sterilization is injected into the head-end fermentation reactor and the tail-end fermentation reactor, so that the quantity of gas qualified for sterilization produced in the air compressor can meet the injection quantity.
Particularly, when the cell density of the microorganisms in the fermentation reactor at the head end exceeds the preset cell density range and the sterilization effect of the air compressor is determined to be poor after pollution points at other sites are discharged, the sterilization efficiency is improved by increasing the temperature conversion frequency of the air compressor.
Drawings
FIG. 1 is a schematic structural diagram of a microbial fermentation tank for improving degradation efficiency according to an embodiment of the present invention;
FIG. 2 is a standard curve for growth of Bacillus according to the present invention;
FIG. 3 is a schematic diagram of a head-end fermentation reactor according to an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a tail-end fermentation reactor according to an embodiment of the present invention;
fig. 5 is a schematic structural diagram of each injection unit according to an embodiment of the present invention.
Detailed Description
In order that the objects and advantages of the invention will be more clearly understood, the invention is further described below with reference to examples; it should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and do not limit the scope of the present invention.
FIG. 1 is a schematic view of a microbial fermentation tank with improved degradation efficiency according to an embodiment of the present invention, including,
the head end fermentation reactor 1 is provided with a barrel-shaped first tank body, the first tank body is provided with a space for fermenting microorganisms and culture solution which are wrapped in a membrane, the bottom in the first tank body is provided with a first gas injection unit 12 for providing gas required in the pre-fermentation process, and the outside of the first tank body is provided with a first heating unit 11;
the tail end fermentation reactor 2 is connected with the head end fermentation reactor, is provided with a second tank body used for fermenting the pre-fermented product generated by the head end fermentation reactor again, the bottom of the second tank body is provided with a second gas injection unit 22 used for fermenting the pre-fermented product again, the outside of the second tank body is provided with a second heating unit 23, and the tail end fermentation reactor also comprises a conduit 24 extending into the first tank body and connected with the bottom of the second tank body and a first pump body 23 used for controlling the amount of the pre-fermented liquid injected into the second tank body;
the air compressor 3 is provided with two exhaust pipelines, each exhaust pipeline injects gas into the head-end fermentation reactor and the tail-end fermentation reactor respectively, wherein a second pump body 31 for controlling the gas injection rate into the head-end fermentation reactor is arranged on the first exhaust pipeline, a third pump body 37 for controlling the gas injection rate into the tail-end fermentation reactor is arranged on the second exhaust pipeline, a sterilization mechanism for sterilizing the injected gas is arranged in the air compressor, heating mechanisms for providing convection for the air in the air compressor are arranged on two sides of the air compressor, the heating mechanisms are connected with the first heating unit and the second heating unit so as to transfer the heat energy of the heating mechanisms to the head-end fermentation reactor and the tail-end fermentation reactor, the heating mechanisms comprise a first heat exchanger 32 for controlling the temperature of a first heating pipe and a second heat exchanger 33 for controlling the temperature of a second heating pipe, a first three-way valve 34 is arranged on the first heating pipe, a second three-way valve 36 is arranged on the second heating pipe, and a reversing valve 35 for mutually converting the heat energy of the first heating unit and the heat energy of the second heating unit is also arranged on the heating mechanism;
and the controller is connected with the head-end fermentation reactor, the tail-end fermentation reactor and the air compressor and is used for injecting the pre-fermentation liquid into the tail-end fermentation reactor when the growth vigor of microorganisms in the head-end fermentation reactor is in a logarithmic growth phase, and adjusting the temperature conversion frequency of the heating mechanism according to the growth rate of the microorganisms in the tail-end fermentation reactor so as to improve the degradation efficiency of the microorganisms in the culture solution.
In use, the attachment attached with microorganisms is fermented in the head end fermentation reactor to the end of the logarithmic growth phase of the microorganisms, and then is injected into the tail end fermentation reactor for re-fermentation, in the fermentation process of the head end fermentation reactor and the tail end fermentation reactor, the air in the air compressor is sterilized and then is respectively injected into the head end fermentation reactor and the tail end fermentation reactor through the exhaust pipelines to stir and supply the fermentation liquid in each fermentation reactor, in order to improve the sterilization efficiency of the gas in the air compressor, the embodiment of the invention is provided with the heating mechanism on the air compressor, the heat exchanger of the heating mechanism heats and cools the air on the air compressor, the convection of the gas in the air compressor is realized, the flowing of the gas is improved, and the sterilization efficiency of the gas is further improved, more specifically, in the embodiment of the invention, the first heat exchanger of the heating mechanism heats liquid with increased temperature to the gas at one side of the air compressor through the heating pipe arranged at one side of the air compressor, the first three-way valve opens the valve passing through the first heating unit, the liquid heated to the gas at one side of the air compressor is injected into the first heating unit, the heated liquid is used for providing heat for the fermentation reactor at the head end, energy resources are fully utilized, and resource waste is avoided, at the moment, the second heat exchanger cools the liquid with decreased temperature to the gas at the other side of the air compressor through the heating pipe arranged at the other side of the air compressor, the second three-way valve opens the valve passing through the second recovery pipeline, the cooled liquid is recovered, after a period of time, the first heat exchanger opens the valve passing through the first recovery pipeline through the heating pipe arranged at one side of the air compressor to the gas at one side of the air compressor through the first three-way valve arranged at one side of the air compressor, liquid after the temperature of the air at one side of the air compressor is reduced is recovered, meanwhile, the liquid with the temperature increased by the second heat exchanger is heated up by the heating pipe arranged at the other side of the air compressor, the valve of the second heating unit is opened by the second three-way valve, and the heated liquid is supplied to the tail end fermentation reactor to be heated up by heat.
Specifically, the invention attaches microorganisms to a membrane, so that the microorganisms attached to attachments can be gathered and grown in a culture solution and can be separated from the culture solution, nutrient substances of the culture solution are used for propagation, meanwhile, the microorganisms on the attachments are separated from the attachments gradually and gradually in the growth and propagation process, so that the microorganisms grow orderly, the microorganisms on the attachments are ensured to be remained for cyclic utilization, the use time of the microorganisms can be prolonged while fermentation is realized, meanwhile, the invention is provided with a head-end fermentation reactor for culturing the microorganisms to a logarithmic growth phase and a tail-end fermentation reactor for stably culturing fermentation products in the logarithmic growth phase, aerobic fermentation is realized by injecting sterilized gas into the head-end fermentation reactor and the tail-end fermentation reactor, the fermentation liquor in each reactor is stirred, and the optimal temperature suitable for the fermentation process is provided for the reactors by using the heat of a heating mechanism for promoting the gas convection in an air compressor, so as to improve the degradation efficiency of the microorganisms in the fermentation liquor.
Specifically, the activated streptococcus thermophilus is produced by culturing streptococcus thermophilus and culture solution for 10 hours at the temperature of 42 ℃ to form activated streptococcus thermophilus by taking a microbial fermentation tank as an example, wherein attachment for growth of the streptococcus thermophilus is arranged in the culture solution, after activation, the attachment with the streptococcus thermophilus is put into a head-end fermentation reactor, propagation is carried out by utilizing nutrient substances of the culture solution in the head-end fermentation reactor, when the in-membrane microbial growth stage in the head-end fermentation reactor is logarithmic phase, pre-fermentation liquid in the head-end fermentation reactor is injected into a tail-end fermentation reactor, mixed fermentation is carried out with culture liquid of the tail-end fermentation reactor, and the yogurt starter is prepared after 5-6 hours, wherein the activated streptococcus thermophilus culture solution comprises 5.0g of lactose, 5.0g of soybean peptone, 6.0g of tryptone, 6.0g of beef extract, 2.0g of yeast powder, 0.5g of ascorbic acid, 0.5g of lactose, 20.0g of beta-glycerol disodium phosphate, a fixed amount of distilled water to 1000.0 g of distilled water, 0.0g of activated streptococcus thermophilus culture solution, 0.0.0 g of calcium carbonate and 0.0g of skimmed water, and 0.0g of calcium carbonate, and 0.8 ml of sterilized culture solution of the activated streptococcus thermophilus culture solution.
Specifically, in the embodiment of the invention, a microbial fermentation tank is adopted to produce a bacillus starter, bacillus is inoculated in a culture medium for activation, the activated bacillus is mixed with a culture solution, attachment for growth of the bacillus is arranged in the culture solution, the attachment attached with the bacillus is put into a head-end fermentation reactor for culture for 4 hours, the microorganism in the head-end fermentation reactor is cultured until a logarithmic growth phase is reached, then the microorganism is injected into a tail-end reaction fermentation phase for secondary fermentation, and the bacillus starter is prepared by fermentation for 6 hours, wherein the activation culture medium comprises 20g of whole milk powder, 10g of agar, 0.05g of manganese sulfate heptahydrate, 20g of calcium carbonate, 5.0g of sodium acetate, 2.0g of diamine citrate, 2.0g of dipotassium hydrogen phosphate, 0.02g of magnesium sulfate heptahydrate and 1.0g of tween 80, the amount of distilled water is 1000ml, the culture solution of the head-end fermentation reactor is 25g of whole milk powder, 0.05g of heptahydrate, 20g of calcium carbonate, 5.0g of sodium acetate, 2.0g of diamine citrate, 2.0g of potassium dipotassium phosphate, 0g of Tween, 0.02g of distilled water, 0g of 100 g of distilled water, 0.02g of calcium sulfate, 1000ml of distilled water and the tail-end fermentation reactor, the culture medium is used as auxiliary raw materials, and the fermentation raw materials are prepared by fermentation, and the fermentation liquid is sterilized, and the fermentation raw materials are fermented milk, and the fermentation is supplemented with the raw materials of the raw materials after fermentation.
Specifically, the embodiment of the invention adopts a microbial fermentation tank which can also be applied to fermented organic fertilizers, decomposed kitchen waste and the like, taking the fermented organic fertilizers as an example, microbial inoculum is activated and adhered to an attachment, the attachment is put into a head-end fermentation reactor, a culture medium in the head-end fermentation reactor is a culture solution suitable for growth of the microbial inoculum, when the cell density change rate of the microbial inoculum in the head-end fermentation reactor reaches a preset standard, the attachment is put into a tail-end fermentation reactor, organic materials to be fermented, such as excrement or straws and the like, are in the tail-end fermentation reactor, the fermented organic fertilizers adopting the embodiment of the invention can be improved in decomposition time of 1-2 days, and the microbial inoculum is adopted to ferment the organic fertilizers for 4-6 days, wherein the temperatures of the head-end fermentation reactor and the tail-end fermentation reactor are set according to the microbial inoculum and appropriate conditions of the fermentation environment.
Wherein the controller compares the cell density of the microorganisms in the first-end fermentation reactor obtained in the preset culture time with the cell density of a microorganism growth standard curve to judge the growth rate of the microorganisms, and when the growth rate does not meet the standard, the controller judges whether to adjust the rotation rate of the first gas injection unit and the gas injection amount according to the temperature of the first-end fermentation reactor or the dispersion degree of the microorganisms, wherein the controller obtains the cell density change rate P of the microorganisms in the first-end fermentation reactor in the preset culture time and compares the cell density change rate P of the microorganisms in the logarithmic growth phase with the microorganism growth standard curve to judge the growth rate of the microorganisms, wherein,
when P is less than P-delta P, the control machine judges that the growth rate of the microorganisms in the first-end fermentation reactor does not meet the standard, and the growth cycle of the microorganisms is not in the logarithmic growth phase;
when the P-delta P is not more than P and not more than P plus delta P, the control machine judges that the growth rate of the microorganisms in the first-end fermentation reactor meets the standard, and the growth cycle is in the logarithmic growth phase;
when P is more than P + delta P, the control machine judges that the growth rate of the microorganisms in the first-end fermentation reactor does not meet the standard;
wherein, the delta p is the cell density change rate error value preset by the controller.
Specifically, the embodiment of the present invention does not limit the determination of the cell density of the microorganism in the head-end fermentation reactor, and the cell density change rate of the microorganism can be characterized by the concentration of the secondary metabolite in the fermentation broth in the head-end fermentation reactor, or can be determined by determining the number of the microorganism cells in the head-end fermentation reactor, and a microorganism growth standard curve is drawn according to the cell density of the microorganism to be cultured in each cultivation time in the cultivation process, taking bacillus as an example, see fig. 2, which is a schematic diagram of the bacillus growth standard curve in the embodiment of the present invention, and the cell density change rate P of the logarithmic growth phase in the bacillus standard curve is obtained to be 1.7, the spore of the secondary metabolite in the bacillus fermentation process is taken as an index of the cell density, the spore content ρ 1 of the bacillus metabolite in the first preset cultivation time point in the head-end fermentation reactor is the cell density of the first preset cultivation time, the spore content ρ 2 of the bacillus metabolite in the second preset cultivation time point is the cell density of the second preset cultivation time point, the spore content ρ 2 of the bacillus metabolite in the third preset cultivation time point in the head-end fermentation reactor is the cell density of the first preset cultivation time point, and the spore density change rate P2-P2 of the second preset cultivation time point is set as the cell density of the third cell density of the microorganism (ρ 3 — P2); when the cell number of bacillus in the fermentation process of bacillus is used as an index of cell density, the cell number rho 1 in the quantitative sampling of bacillus at a first preset culture time point in a head-end fermentation reactor is the cell density at the first preset culture time point, the cell number rho 2 in the quantitative sampling of bacillus at a second preset culture time point is the cell density at the second preset culture time point, the cell number rho 3 in the quantitative sampling of bacillus at a third preset culture time point is the cell density at the third preset culture time point, and the change rate p = (rho 3-rho 2)/(rho 2-rho 1) of microbial cell density is set.
Specifically, the method comprises the steps of evaluating the growth rate of microorganisms in the head-end fermentation reactor according to the comparison of the cell density change rate of the microorganisms in the head-end fermentation reactor with the cell density change rate of a growth logarithmic phase in a standard curve, and simultaneously judging whether the microorganisms are in the growth logarithmic phase, wherein if the cell density change rate of the microorganisms is smaller than the error range of the cell density change rate of the growth logarithmic phase of the standard curve, the growth rate of the microorganisms in the head-end fermentation reactor is not in accordance with the standard, and if the cell density change rate of the microorganisms is lower than the standard value, the growth rate of the microorganisms in the head-end fermentation reactor is proved to be not to reach the logarithmic phase, the efficiency of the current head-end fermentation reactor is too low, if the cell density change amount of the microorganisms is larger than the error range of the cell density change rate of the logarithmic phase of the growth logarithmic phase of the standard curve, the condition that the microorganisms are possibly introduced is represented, the microorganisms are detected, and if the cell density change rate of the microorganisms is in the logarithmic phase of the standard, the microorganisms in the tail-end fermentation reactor is proved to be in accordance with the standard, and the growth cycle of the microorganisms are injected into the fermentation liquid.
Under a first condition, the control machine increases the first temperature of the first heat exchanger of the heating mechanism according to the temperature conversion frequency F of the first heat exchanger and the second heat exchanger of the heating mechanism so as to increase the temperature of the head-end fermentation reactor, wherein when F is less than or equal to F, the control machine increases the first temperature w1 to w11 of the first heat exchanger by adopting a first adjusting parameter k1, and w11= w1 × k1 is set; when F is larger than F, the control machine opens a reversing valve connected between the first heating unit and the second heating unit to transfer the heat of the second heating unit to the first heating unit;
the first condition is that the fermentation temperature of the first-end fermentation reactor is lower than a preset pre-fermentation temperature, the growth cycle of microorganisms in the first-end fermentation reactor is not in a logarithmic growth phase, and the control machine presets a temperature conversion frequency F, wherein k1= k0 x (1 + (F-F)/F0), k0 is a preset regulation parameter standard value, and F0 is a preset temperature conversion frequency standard value.
Specifically, under the condition that the growth cycle of microorganisms in the first-end fermentation reactor does not reach the logarithmic phase of growth within the preset time and the condition that the temperature of the first-end fermentation reactor is too low is detected, the problem that the temperature of the first-end fermentation reactor is too low due to too high temperature conversion frequency and the growth rate of the microorganisms cultured by the first-end fermentation reactor is too low is solved by correcting the first temperature of the first heat exchanger according to the temperature conversion frequency of the first heat exchanger and the temperature conversion frequency of the second heat exchanger.
Specifically, the embodiment of the present invention does not limit the temperature conversion frequency of the air compressor, and it is required to specifically set according to the amount of gas that is sterilized in one time in the air compressor, and the embodiment of the present invention provides a preferred embodiment, when fermenting bacillus, the optimum temperature in the head-end fermentation reactor is 30 ℃, the optimum temperature in the tail-end fermentation reactor is 35 ℃, the high temperature of the first heat exchanger, i.e., the first temperature of the first heat exchanger, is set to 35 to 40 ℃, the low temperature of the first heat exchanger, i.e., the second temperature of the first heat exchanger, is set to 25 to 30 ℃, the high temperature of the second heat exchanger, i.e., the first temperature of the second heat exchanger, is set to 40 to 45 ℃, the low temperature of the second heat exchanger, i.e., the second temperature of the second heat exchanger, is set to 25 to 30 ℃, and the high and low temperature exchange time of each heat exchanger in the heating mechanism, i.e., 5 to 8min, is the air injection amount per unit time that the temperature conversion frequency of the air compressor can be 1L volume.
Under a second condition, the control machine adjusts the rotation speed and the gas injection speed of the first gas injection unit according to the microbial dispersion degree Y in the head-end fermentation reactor, wherein when Y is less than or equal to Y, the control machine increases the gas injection speed of the first gas injection unit by vs to vs1, and sets vs1= vs x (1 + (Y-Y)/Y); when Y is larger than Y, the control machine increases the rotation speed cs of the first gas injection unit to cs1, and sets cs1= cs x (1 + (Y-Y)/Y);
and the second condition is that the fermentation temperature of the first-end fermentation reactor is higher than the preset pre-fermentation temperature, the growth cycle of the microorganisms in the first-end fermentation reactor is not in the logarithmic growth phase, and the dispersion degree standard value Y is preset by the controller.
Specifically, the fermentation temperature in the first-end fermentation reactor is determined to be higher than the preset pre-fermentation temperature, which indicates that the reason that the growth period of microorganisms in the first-end fermentation reactor does not reach the logarithmic growth phase within the preset time is not the fermentation temperature, but the reason that the microorganisms in the first-end fermentation reactor are not uniformly mixed with the culture solution or the gas injection amount is insufficient is that the dispersion degree of the microorganisms in the first-end fermentation reactor is not uniform or the gas injection amount is insufficient.
Specifically, the embodiment of the invention obtains the cell density of each point by sampling each point in the head-end fermentation reactor to determine the dispersion degree of the microorganisms according to the variance formula, and more specifically, the embodiment of the invention obtains the cell density of each point in the head-end fermentation reactor to be 6.2 multiplied by 10 5 CFU/ml、5.9×10 5 CFU/ml、7.1×10 5 CFU/ml、6.0×10 5 CFU/ml、5.8×10 5 CFU/ml, the real-time microbial dispersion was obtained as ((6.2-6.2) 2 +（5.9-6.2） 2 +（7.1-6.2） 2 +（6.0-6.2） 2 +（5.8-6.2） 2 ) And/5 =0.22, the dispersion degree is less than the preset dispersion degree of 0.8, namely the standard is met.
Please refer to fig. 3, which is a schematic structural diagram of a first-end fermentation reactor according to an embodiment of the present invention, the first-end fermentation reactor includes a microorganism injection unit, the microorganism injection unit includes a driver 13 for controlling a rotation speed of an injector 14, the controller presets a first maximum rotation speed csmax of an injection unit, an adjusted rotation speed cs1 of the first injection unit is greater than the preset maximum rotation speed csmax of the first injection unit, and the controller determines to set the rotation speed of the first injection unit to csmax, and simultaneously increases a rotation speed vb at which a next microorganism is injected to vb1, and sets vb1= vb × (1 + (cs 1-csmax)/csmax).
With continuing reference to fig. 3, the first-end fermentation reactor is provided with a plurality of sampling ports for detecting the cell density of the microorganisms, wherein the first sampling port 15, the second sampling port 16 and the third sampling port 17 are used for detecting the cell density of the microorganisms at each point of the first-end fermentation reactor.
Specifically, the microorganism injection unit capable of controlling the injection rotating speed is arranged at the top of the head-end fermentation reactor, and when the rotating speed of the first gas injection unit reaches the maximum, in order to avoid the microbial cell rupture caused by the overlarge rotating speed of the first gas injection unit, the controller adjusts the rotating speed of the first gas injection unit to the preset maximum value and controls and improves the rotating speed of the microorganism injection unit so as to improve the initial dispersion degree of microorganisms in the next pre-fermentation process.
And after the pre-fermented product of the head-end fermentation reactor is injected into the tail-end fermentation reactor, detecting the microbial growth rate of the tail-end fermentation reactor after the pre-fermented product is subjected to preset re-fermentation time, comparing the obtained microbial growth rate H of the tail-end fermentation reactor with a preset growth rate H by a control machine, if the microbial growth rate of the tail-end fermentation reactor is greater than the preset growth rate, not adjusting the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit by the control machine, and if the microbial growth rate of the tail-end fermentation reactor is less than the preset growth rate, judging whether the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit are adjusted by the control machine according to the temperature of the tail-end fermentation reactor.
Wherein when the growth rate of the microorganisms in the tail end fermentation reactor is less than the preset growth rate, the controller obtains the temperature D of the tail end fermentation reactor to compare with the preset re-fermentation temperature D, and judges whether to adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit, wherein,
when D is less than or equal to D, the control machine determines to increase the first temperature w2 of the second heat exchanger to w21, and sets w21= w2 × (1 + (D-D)/D);
when D > D, the control machine increases the rotation rate cw to cw1 of the second gas injection unit, sets cw1= cw x (1 + (D-D)/D), and increases the gas injection rate vw to vw1 of the second gas injection unit, sets vw1= vwx (1 + (D-D)/D).
Specifically, the invention detects the microorganism growth rate of the tail end fermentation reactor for re-fermentation, compares the obtained microorganism growth rate of the tail end fermentation reactor with a preset growth rate, and judges whether the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit are adjusted according to the temperature of the tail end fermentation reactor, wherein if the microorganism growth rate of the tail end fermentation reactor is greater than the preset growth rate, the current microorganism growth rate of the tail end fermentation reactor is judged to meet the standard, and if the microorganism growth rate of the tail end fermentation reactor is less than the preset growth rate, the current microorganism growth rate of the tail end fermentation reactor is judged to not meet the standard, the controller compares the temperature of the tail end fermentation reactor with the preset re-fermentation temperature to determine the reason that the microorganism growth rate of the tail end fermentation reactor does not meet the standard, if the temperature of the tail end fermentation reactor is lower than the preset growth rate, the high temperature of the second heat exchanger is increased to increase the heating temperature of the second heating unit, thereby increasing the re-fermentation temperature of the tail end fermentation reactor, and if the temperature of the tail end fermentation reactor is higher than the preset fermentation reaction temperature, the rotation rate of the tail end fermentation reactor, the gas injection rate of the tail end fermentation reactor is increased, and the aeration rate of the re-fermentation reactor is increased, thereby the propagation rate of the fermentation reactor is increased.
Please refer to fig. 4, which is a schematic structural diagram of a fermentation reactor at the end of the present invention, in which at least one ultrasonic generator is disposed on an outer wall of the fermentation reactor at the end for promoting microbial fermentation, the controller presets a second rotation speed cwmax of the gas injection unit, when the adjusted rotation speed cw1 of the second gas injection unit is greater than a preset maximum value cwmax of the second rotation speed cwmax of the gas injection unit, the controller sets the rotation speed cw1 of the second gas injection unit to cwmax, and starts the ultrasonic generator to adjust the frequency E of the ultrasonic generator to E1, and sets E1= E x (1 + (cw 1-cwmax)/cwmax).
Referring to fig. 4, in the embodiment of the present invention, 5 ultrasonic generators are provided, and are respectively disposed on the outer wall of the tail-end fermentation reactor at intervals, including a first ultrasonic generator 25, a second ultrasonic generator 26, a third ultrasonic generator 27, a fourth ultrasonic generator 28, and a fifth ultrasonic generator 29, the number of the ultrasonic generators is not limited in the embodiment of the present invention, as long as the ultrasonic generators can provide ultrasonic waves to the microorganisms to be fermented in the tail-end fermentation reactor, and the frequency of the ultrasonic generators is 20 khz.
Specifically, the tail end fermentation reactor is provided with the plurality of ultrasonic generators, the ultrasonic generators have the functions of oscillating and strengthening culture on microorganisms in the tail end fermentation reactor, the control machine obtains that the rotation speed of the second gas injection unit exceeds the maximum value of the preset second gas injection unit rotation speed, and the control machine starts the ultrasonic generators and increases the frequency of the ultrasonic generators according to the difference value of the regulated rotation speed and the preset second gas injection unit rotation speed so as to promote the fermentation of the microorganisms in the tail end fermentation reactor.
And the control machine shortens the temperature conversion frequency f0 to f1 of the air compressor according to the adjusted injection speed vs1 of the first gas injection unit and the adjusted gas injection speed vw1 of the second gas injection unit, and sets f1= fx (1-0.5 x (vs 1-vs)/vs x 0.8 x (vw 1-vw)/vw).
Specifically, in order to prevent the amount of gas sterilized in the air compressor from reaching the injection amount into each fermentation reactor, the controller adjusts the temperature conversion frequency of the air compressor according to the obtained and adjusted gas injection rate of the first gas injection unit and the gas injection rate of the second gas injection unit, so that the convection rate in the air compressor is increased, the gas sterilization rate in the air compressor is increased, and a large amount of gas which is qualified in sterilization is ensured to be injected into the head-end fermentation reactor and the tail-end fermentation reactor, so that the amount of gas which is qualified in sterilization and is produced in the air compressor can meet the injection amount.
And under a third condition, the control machine increases the temperature conversion frequency fq to fq1 of the air compressor, sets fq1= fq × 1.25, sets the third condition that P is greater than P +. DELTA.p, and judges that the air compressor does not meet the standard for sterilization, and sets q =0,1.
Specifically, when the microbial cell density in the fermentation reactor at the head end is obtained and exceeds the preset cell density range, and the sterilization effect of the air compressor is determined to be poor after pollution points at other sites are discharged, the temperature conversion frequency of the air compressor is increased to improve the sterilization efficiency.
Referring to fig. 5, which is a schematic structural diagram of each injection unit according to an embodiment of the present invention, the injection unit includes a first gear 43, a second gear 42 engaged with the first gear, and an air injection pipe 41 disposed on the second gear, wherein, in use, a motor disposed below the first gear controls the first gear to rotate, so as to drive the second gear to rotate along the first gear, thereby implementing air injection of the air injection pipe at each position in each fermentation reactor. The first gas injection unit is similar to the second gas injection unit in structure, and gears with different sizes are arranged according to the fermentation spaces of the head-end fermentation reactor and the tail-end fermentation reactor.
So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of the present invention is obviously not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can be within the protection scope of the invention.

Claims (10)

1. A microbial fermentation tank for improving degradation efficiency is characterized by comprising:
the head end fermentation reactor is provided with a barrel-shaped first tank body, the first tank body is provided with a space for fermentation of microorganisms and culture solution wrapped in a membrane, a first gas injection unit for providing gas required in the pre-fermentation process is arranged at the bottom in the first tank body, and a first heating unit is arranged outside the first tank body;
the tail end fermentation reactor is connected with the head end fermentation reactor and is provided with a second tank body used for fermenting the pre-fermented product generated by the head end fermentation reactor again, a second gas injection unit used for fermenting the pre-fermented product again is arranged at the bottom of the second tank body, and a second heating unit is arranged outside the second tank body;
the air compressor is provided with two exhaust pipelines, each exhaust pipeline injects gas into the head-end fermentation reactor and the tail-end fermentation reactor respectively, a sterilization mechanism for sterilizing the injected gas is arranged in the air compressor, heating mechanisms for providing convection for the air in the air compressor are arranged on two sides of the air compressor, and the heating mechanisms are connected with the first heating unit and the second heating unit so as to transfer the heat energy of the heating mechanisms to the head-end fermentation reactor and the tail-end fermentation reactor;
and the controller is connected with the head-end fermentation reactor, the tail-end fermentation reactor and the air compressor and is used for injecting a pre-fermentation liquid into the tail-end fermentation reactor when the growth vigor of microorganisms in the head-end fermentation reactor is in a logarithmic growth phase, and adjusting the temperature conversion frequency of the heating mechanism according to the growth rate of the microorganisms in the tail-end fermentation reactor so as to improve the degradation efficiency of the microorganisms in the culture solution.
2. The microbial fermentation tank with improved degradation efficiency as claimed in claim 1, wherein the controller compares the cell density of the microbes in the head-end fermentation reactor obtained by the preset cultivation time with the cell density of a microbial growth standard curve to determine the microbial growth rate, and when the growth rate does not meet the standard, the controller determines whether to adjust the rotation rate of the first gas injection unit and the gas injection amount according to the temperature of the head-end fermentation reactor or the dispersion degree of the microbes, wherein the controller obtains the cell density change rate P of the microbes in the head-end fermentation reactor obtained by the preset cultivation time and compares the cell density change rate P of the microbes in the logarithmic growth phase of the microbial growth standard curve to determine the growth rate of the microbes,
when P is less than P-delta P, the control machine judges that the growth rate of the microorganisms in the first-end fermentation reactor does not meet the standard, and the growth cycle of the microorganisms is not in the logarithmic growth phase;
when the P-delta P is not less than the P + delta P, the control machine judges that the growth rate of the microorganisms in the first-end fermentation reactor meets the standard, and the growth cycle of the microorganisms is in the logarithmic growth phase;
when P is more than P + delta P, the control machine judges that the growth rate of the microorganisms in the first-end fermentation reactor does not meet the standard;
wherein, the delta p is the cell density change rate error value preset by the controller.
3. The microbial fermentation tank with improved degradation efficiency as claimed in claim 2, wherein the control machine increases the first temperature of the first heat exchanger of the heating mechanism according to the temperature conversion frequency F of the first heat exchanger and the second heat exchanger of the heating mechanism under the first condition to increase the temperature of the head-end fermentation reactor, wherein when F is less than or equal to F, the control machine increases the first temperature w1 to w11 of the first heat exchanger by using a first adjustment parameter k1, and the setting is w11= w1 × k1; when F is larger than F, the control machine opens a reversing valve connected between the first heating unit and the second heating unit to transfer the heat of the second heating unit to the first heating unit;
the first condition is that the fermentation temperature of the first-end fermentation reactor is lower than a preset pre-fermentation temperature, the growth cycle of microorganisms in the first-end fermentation reactor is not in a logarithmic growth phase, and the control machine presets a temperature conversion frequency F, wherein k1= k0 x (1 + (F-F)/F0), k0 is a preset adjusting parameter standard value, and F0 is a preset temperature conversion frequency standard value.
4. The microbial fermentation tank with improved degradation efficiency of claim 3, wherein the controller adjusts the rotation rate and the gas injection rate of the first gas injection unit according to the microbial dispersion degree Y in the head-end fermentation reactor under the second condition, wherein when Y is less than or equal to Y, the controller increases the gas injection rate vs to vs1 of the first gas injection unit, and sets vs1= vs x (1 + (Y-Y)/Y); when Y is larger than Y, the control machine increases the rotation speed cs of the first gas injection unit to cs1, and sets cs1= cs x (1 + (Y-Y)/Y);
and under the second condition that the fermentation temperature of the first-end fermentation reactor is higher than the preset pre-fermentation temperature and the growth cycle of microorganisms in the first-end fermentation reactor is not in the logarithmic phase, the dispersion degree standard value Y is preset by the controller.
5. The microbial fermentation tank with improved degradation efficiency according to claim 4, wherein the head-end fermentation reactor comprises a microbial injection unit, the microbial injection unit comprises a driver for controlling the rotation rate of the injector, the controller presets a first maximum rotation rate csmax of the gas injection unit, the adjusted rotation rate cs1 of the first gas injection unit is greater than the preset maximum rotation rate csmax of the first gas injection unit, and the controller determines that the rotation rate of the first gas injection unit is set to csmax, and simultaneously increases the rotation rate vb at the next microbial injection to vb1, and sets vb1= vb x (1 + (cs 1-csmax)/csmax).
6. The microbial fermentation tank with improved degradation efficiency as claimed in claim 5, wherein the controller compares the obtained microorganism growth rate H of the tail-end fermentation reactor with a preset growth rate H after the pre-fermentation time of the head-end fermentation reactor has elapsed since the pre-fermentation time of the tail-end fermentation reactor was injected into the tail-end fermentation reactor, and if the microorganism growth rate of the tail-end fermentation reactor is greater than the preset growth rate, the controller does not adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit, and if the microorganism growth rate of the tail-end fermentation reactor is less than the preset growth rate, the controller determines whether to adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit according to the temperature of the tail-end fermentation reactor.
7. The microbial fermentation tank with improved degradation efficiency as claimed in claim 6, wherein when the microbial growth rate of the tail-end fermentation reactor is lower than a preset growth rate, the controller obtains the temperature D of the tail-end fermentation reactor and compares the temperature D with a preset re-fermentation temperature D to determine whether to adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit, wherein,
when D is less than or equal to D, the control machine determines to increase the first temperature w2 of the second heat exchanger to w21, and sets w21= w2 × (1 + (D-D)/D);
when D > D, the control machine increases the rotation speed cw to cw1 of the second gas injection unit, sets cw1= cw × (1 + (D-D)/D), and simultaneously increases the gas injection speed vw to vw1 of the second gas injection unit, sets vw1= vwx (1 + (D-D)/D).
8. The microbial fermentation tank with improved degradation efficiency according to claim 7, wherein at least one ultrasonic generator is disposed on the outer wall of the tail end fermentation reactor for promoting microbial fermentation, the control machine presets a second gas injection unit rotation rate cwmax, when the adjusted second gas injection unit rotation rate cw1 is greater than a preset second gas injection unit rotation rate maximum cwmax, the control machine sets the second gas injection unit rotation rate cwmax, and starts the ultrasonic generator to adjust the frequency E of the ultrasonic generator to E1, and sets E1= E x (1 + (cw 1-cwmax)/cwmax).
9. The microbial fermentation tank with improved degradation efficiency according to claim 8, wherein the control machine sets f1= fx (1-0.5 x (vs 1-vs)/vs x 0.8 x (vw 1-vw)/vw) by shortening the temperature change frequency f0 to f1 of the air compressor according to the adjusted injection rate vs1 of the first gas injection unit and the injection rate vw1 of the second gas injection unit.
10. The microbial fermentation tank with improved degradation efficiency as claimed in claim 9, wherein the control engine increases the temperature conversion frequency fq to fq1 of the air compressor under a third condition, fq1= fq × 1.25, q =0,1, the third condition is that P > P +. DELTA.p, and the control engine determines that the air compressor sterilization does not meet the criterion.
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