CN115627215B - Microbial fermentation tank capable of improving degradation efficiency - Google Patents
Microbial fermentation tank capable of improving degradation efficiency Download PDFInfo
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- CN115627215B CN115627215B CN202211569845.3A CN202211569845A CN115627215B CN 115627215 B CN115627215 B CN 115627215B CN 202211569845 A CN202211569845 A CN 202211569845A CN 115627215 B CN115627215 B CN 115627215B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
Abstract
The invention relates to the field of fermentation, and relates to a microbial fermentation tank for improving degradation efficiency, which comprises a head end fermentation reactor, a first fermentation tank and a second fermentation tank, wherein the head end fermentation reactor is provided with a barrel-shaped first tank body, and the bottom in the first tank body is provided with a first gas injection unit for providing gas required in a pre-fermentation process; the tail end fermentation reactor is provided with a second tank body for secondary fermentation of the pre-fermented product generated by the head end fermentation reactor, the bottom of the second tank body is provided with a second gas injection unit for secondary fermentation of the pre-fermented product, and the outside of the second tank body is provided with a second heating unit; an air compressor provided with a sterilization mechanism for sterilizing the injected gas; and the control machine is used for injecting the pre-fermentation liquid into the tail end fermentation reactor when the growth vigor of the microorganisms in the head end fermentation reactor is in the logarithmic phase, and adjusting the temperature conversion frequency of the heating mechanism according to the growth rate of the microorganisms in the tail end fermentation reactor so as to improve the degradation efficiency of the microorganisms in the culture liquid.
Description
Technical Field
The invention relates to the field of fermentation, in particular to a microbial fermentation tank for improving degradation efficiency.
Background
Microbial fermentation refers to the process of converting a feedstock into a product desired by a human being via a specific metabolic pathway under suitable conditions using microorganisms. The fermentation production level of microorganisms is mainly dependent on the genetic characteristics of the strain itself and the culture conditions. The microbial fermentation process is divided into aerobic fermentation and anaerobic fermentation according to the requirements of fermentation conditions. The aerobic fermentation method comprises the steps of liquid surface culture fermentation, fermentation on the surface of a porous or granular solid culture medium, oxygen-introducing submerged fermentation and the like. The influencing factors of the aerobic fermentation are mainly the temperature and oxygen content suitable for the growth of microorganisms and uniform oxygen transmission.
Chinese patent CN 113213992B discloses a microbial fermentation reation kettle, through setting up the reation kettle body and installing the reation kettle lid on the reation kettle body, install drive arrangement on the reation kettle lid, be equipped with the import and export device on the reation kettle body in order to reach the stirring efficiency that improves the raw materials, also enable the raw materials to carry out abundant purpose of stirring in the reation kettle simultaneously, still do not solve the technical problem of how to control fermentation temperature and oxygen injection condition in the aerobic fermentation process in order to improve fermentation efficiency.
Disclosure of Invention
Therefore, the invention provides a microbial fermentation tank for improving degradation efficiency, which can solve the technical problem that the residual heat in the use process of an air compressor can not be utilized to improve the microbial fermentation efficiency in the microbial fermentation tank.
In order to achieve the above object, the present invention provides a microbial fermenter for improving degradation efficiency, comprising:
the head end fermentation reactor is provided with a barrel-shaped first tank body, the first tank body is provided with a space for fermenting microorganisms and culture solution wrapped in a membrane, the bottom in the first tank body is provided with a first gas injection unit for providing gas required in the pre-fermentation process, and the outside of the first tank body is provided with a first heating unit;
the tail end fermentation reactor is connected with the head end fermentation reactor and is provided with a second tank body for secondary fermentation of the pre-fermented product generated by the head end fermentation reactor, the bottom of the second tank body is provided with a second gas injection unit for secondary fermentation of the pre-fermented product, and the outer part of the second tank body is provided with a second heating unit;
the air compressor is provided with two exhaust pipelines, each exhaust pipeline respectively injects gas into the head end fermentation reactor and the tail end fermentation reactor, a sterilization mechanism for sterilizing the injected gas is arranged in the air compressor, heating mechanisms for providing convection for air in the air compressor are arranged on two sides of the air compressor, and the heating mechanisms are connected with the first heating unit and the second heating unit so as to transfer heat energy of the heating mechanisms to the head end fermentation reactor and the tail end fermentation reactor;
The control machine is connected with the head end fermentation reactor, the tail end fermentation reactor and the air compressor and is used for injecting the pre-fermentation liquid into the tail end fermentation reactor when the growth vigor of microorganisms in the head end fermentation reactor is in the logarithmic phase, and adjusting the temperature conversion frequency of the heating mechanism according to the growth rate of the microorganisms in the tail end fermentation reactor so as to improve the degradation efficiency of the microorganisms in the culture liquid.
Further, the control machine compares the microbial cell density in the head end fermentation reactor obtained in the preset culture time with the cell density of a microbial growth standard curve to judge the microbial growth rate, when the growth rate does not meet the standard, the control machine judges whether to adjust the rotation rate of the first gas injection unit and the gas injection amount according to the temperature of the head end fermentation reactor or the dispersion degree of the microbes, wherein the control machine obtains the change rate P of the microbial cell density in the head end fermentation reactor in the preset culture time and the change rate P of the microbial cell density in the logarithmic growth phase of the microbial growth standard curve to judge the growth rate of the microbes,
When P is less than P-delta P, the controller judges that the growth rate of microorganisms in the head end fermentation reactor does not meet the standard, and the growth period is not in the logarithmic growth phase;
when P-delta P is less than or equal to P and less than or equal to P+ [ delta ] P, the control machine judges that the growth rate of microorganisms in the head end fermentation reactor meets the standard, and the growth period is in the logarithmic growth phase;
when P is more than P+ [ delta ] P, the controller judges that the microorganism growth rate in the head end fermentation reactor does not meet the standard;
wherein Δp is the error value of the cell density change rate preset by the controller.
Further, the controller increases the first temperature of the first heat exchanger of the heating mechanism according to the temperature conversion frequency F of the first heat exchanger and the second heat exchanger of the heating mechanism under the first condition to increase the temperature of the head end fermentation reactor, wherein when F is less than or equal to F, the controller increases the first temperature w1 to w11 of the first heat exchanger by adopting a first adjustment parameter k1, and w11=w1×k1 is set; when F is more than F, the control machine opens a reversing valve connected between the first heating unit and the second heating unit, and transmits heat of the second heating unit to the first heating unit;
the first condition is that the fermentation temperature of the head-end fermentation reactor is lower than a preset fermentation temperature, the growth period of microorganisms in the head-end fermentation reactor is not in a logarithmic growth phase, the controller presets a temperature conversion frequency F, k1=k0× (1+ (F-F)/F0), wherein k0 is a preset regulation parameter standard value, and F0 is a preset temperature conversion frequency standard value.
Further, the control machine adjusts the rotation rate and the gas injection rate of the first gas injection unit according to the microbial dispersion degree Y in the head end fermentation reactor under a second condition, wherein when Y is less than or equal to Y, the control machine increases the gas injection rate vs to vs1 of the first gas injection unit, and vs1 = vs× (1+ (Y-Y)/Y) is set; when Y is more than Y, the controller increases the rotation rates cs to cs1 of the first gas injection unit, and cs1=cs× (1+ (Y-Y)/Y) is set;
the second condition is that the fermentation temperature of the head end fermentation reactor is higher than a preset pre-fermentation temperature, the growth period of microorganisms in the head end fermentation reactor is not in the logarithmic growth phase, and the controller presets a dispersion degree standard value Y.
Further, the head end fermentation reactor comprises a microorganism injection unit, the microorganism injection unit comprises a driver for controlling the rotation rate of the injector, the controller presets a maximum value csmax of the rotation rate of the first gas injection unit, the rotation rate cs1 of the first gas injection unit after adjustment is larger than the preset maximum value csmax of the rotation rate of the first gas injection unit, the controller judges that the rotation rate of the first gas injection unit is set as csmax, meanwhile, the rotation rate vb of the next microorganism injection is increased to vb1, and vb1=vbx (1+ (cs 1-csmax)/csmax) is set.
Further, after the pre-fermented product of the head end fermentation reactor is injected into the tail end fermentation reactor, the microbial growth rate of the tail end fermentation reactor is detected through the preset re-fermentation time, the control machine compares the obtained microbial growth rate H of the tail end fermentation reactor with the preset growth rate H, if the microbial growth rate of the tail end fermentation reactor is larger than the preset growth rate, the control machine does not adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit, and if the microbial growth rate of the tail end fermentation reactor is smaller than the preset growth rate, the control machine judges whether to adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit according to the temperature of the tail end fermentation reactor.
Further, when the microorganism growth rate of the tail end fermentation reactor is smaller than the preset growth rate, the control machine obtains the temperature D of the tail end fermentation reactor and compares the temperature D with the preset re-fermentation temperature D, and judges whether to adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit,
when D is less than or equal to D, the controller judges that the first temperature w2 of the second heat exchanger is increased to w21, and w21=w2× (1+ (D-D)/D) is set;
When D > D, the controller increases the rotation rate cw to cw1 of the second gas injection unit, sets cw1=cw× (1+ (D-D)/D), and simultaneously increases the gas injection rate vw to vw1 of the second gas injection unit, sets vw1=vw× (1+ (D-D)/D).
Further, at least one ultrasonic generator is arranged on the outer wall of the tail end fermentation reactor and used for promoting microbial fermentation, the controller presets the rotation rate cwmax of the second gas injection unit, when the rotation rate cw1 of the second gas injection unit after adjustment is larger than the preset maximum cwmax of the rotation rate of the second gas injection unit, the controller sets the rotation rate of the second gas injection unit as cwmax, and starts the ultrasonic generator, the frequency E of the ultrasonic generator is adjusted to E1, and E1=E× (cw 1-cwmax)/cwmax is set.
Further, the controller shortens the temperature conversion frequencies f0 to f1 of the air compressor according to the adjusted injection rate vs1 of the first air injection unit and the adjusted injection rate vw1 of the second air injection unit, and sets f1=f× (1-0.5× (vs 1-vs)/vs×0.8× (vw 1-vw)/vw).
Further, the controller increases the temperature conversion frequency fq to fq1 of the air compressor under a third condition that P > p+ [ delta ] P, where fq1=fq×1.25 is set, and the controller determines that the air compressor sterilization does not meet the standard, and q=0, 1.
Compared with the prior art, the invention has the beneficial effects that the microorganisms are attached to the membrane, the attached microorganisms can be gathered and grown in the culture solution and are separated from the culture solution, the nutrient substances of the culture solution are utilized for propagation, the attached microorganisms are separated from the attached microorganisms step by step in the growth and propagation process, so that the microorganisms orderly grow, the attached microorganisms are ensured to remain for recycling, the fermentation is realized, the service time of the microorganisms can be prolonged, and meanwhile, the invention is provided with the head fermentation reactor for culturing the microorganisms to the logarithmic phase and the tail fermentation reactor for stably culturing the fermentation products in the logarithmic phase, and the aerobic fermentation is realized by injecting sterilized gas into the head fermentation reactor and the tail fermentation reactor and stirring the fermentation solution in each reactor, and the heat of the heating mechanism for promoting the gas in the air compressor is utilized for providing the reactor with the optimal temperature suitable for the fermentation process, so as to improve the degradation efficiency of the microorganisms in the fermentation solution.
In particular, the invention evaluates the growth rate of the microorganism in the head-end fermentation reactor according to the comparison of the cell density change rate of the microorganism in the head-end fermentation reactor and the cell density change rate in the growth log phase in a standard curve, and judges whether the microorganism is in the growth log phase, wherein if the microorganism cell density change rate is smaller than the error range of the cell density change rate in the growth log phase of the standard curve, the microorganism growth rate in the head-end fermentation reactor is not in accordance with the standard, the lower standard value proves that the microorganism growth rate does not reach the logarithmic growth phase, the efficiency of the current head-end fermentation reactor is too low, if the microorganism cell density change amount is larger than the error range of the cell density change rate in the growth log phase of the standard curve, the current growth rate is too fast, the condition that foreign bacteria possibly exist is indicated, the purity of the microorganism is detected, and if the microorganism cell density change rate is in the error range of the cell density change rate in the growth log phase of the standard curve, the microorganism growth rate in the head-end fermentation reactor is in accordance with the standard curve, and the growth cycle is in the logarithmic growth phase, the microorganism is infused into the tail-end fermentation broth for fermentation.
In particular, under the condition that the growth period of microorganisms in the head-end fermentation reactor does not reach the growth log period within the preset time and the temperature of the head-end fermentation reactor is detected to be too low, the invention corrects the high temperature of the first heat exchanger according to the temperature conversion frequency of the first heat exchanger and the second heat exchanger so as to avoid the problem that the temperature of the head-end fermentation reactor is too low due to the too fast temperature conversion frequency and the growth rate of the cultured microorganisms is influenced, wherein the controller acquires that the temperature conversion frequency is lower than the preset temperature conversion frequency, the reason that the temperature of the second heating unit is too low is not because the temperature retention time caused by the temperature conversion frequency is short, but because the set temperature of the first heat exchanger is too low, therefore, the controller selects the largest regulating parameter to increase the high-temperature of the first heat exchanger, so as to improve the temperature of the head-end fermentation reactor, and when the temperature conversion frequency of the air compressor exceeds the preset temperature conversion frequency, the controller indicates that the fermentation temperature of the head-end fermentation reactor cannot reach the standard currently, because the temperature conversion frequency is too fast, the heating time of the first heating unit to the head-end fermentation reactor is shortened, the controller judges to open a reversing valve connected between the first heating unit and the second heating unit, the heat of the second heating unit is transferred to the first heating unit, and the heating time of the first heating unit to the head-end fermentation reactor is prolonged.
In particular, in the invention, when the fermentation temperature in the head-end fermentation reactor is determined to be higher than the preset pre-fermentation temperature, the reason that the growth period of microorganisms in the head-end fermentation reactor does not reach the logarithmic growth period within the preset time is not the fermentation temperature, but the reason that the microorganisms in the head-end fermentation reactor are unevenly mixed with the culture solution or the gas injection amount is insufficient is that the control machine obtains the dispersion degree of the microorganisms in the head-end fermentation reactor to be compared with the preset dispersion degree, and the dispersion degree accords with the standard, namely, the dispersion degree of the microorganisms in the head-end fermentation reactor is smaller than or equal to the preset dispersion degree, and the gas injection amount in the head-end fermentation reactor is regulated and controlled by improving the gas injection rate of the first gas injection unit, and when the dispersion degree does not accord with the standard, the dispersion degree of the microorganisms in the head-end fermentation reactor accords with the standard by improving the rotation rate of the first gas injection unit.
In particular, the microorganism injection unit capable of controlling the injection rotation speed is arranged at the top of the head end fermentation reactor, and when the rotation speed of the first gas injection unit reaches the maximum, the control machine adjusts the rotation speed of the first gas injection unit to the preset maximum value and controls the rotation speed of the microorganism injection unit to be improved so as to improve the initial dispersion degree of microorganisms in the next pre-fermentation process in order to avoid the breakage of microorganism cells caused by the overlarge rotation speed of the first gas injection unit.
In particular, the invention detects the microorganism growth rate of the tail end fermentation reactor of the re-fermentation, compares the obtained microorganism growth rate of the tail end fermentation reactor with a preset growth rate, judges whether the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit are regulated according to the temperature of the tail end fermentation reactor, wherein the microorganism growth rate of the tail end fermentation reactor is higher than the preset growth rate, judges that the microorganism growth rate of the current tail end fermentation reactor meets the standard, judges that the microorganism growth rate of the current tail end fermentation reactor does not meet the standard when the microorganism growth rate of the tail end fermentation reactor is lower than the preset growth rate, compares the temperature of the tail end fermentation reactor with the preset re-fermentation temperature to determine the reason that the microorganism growth rate of the tail end fermentation reactor does not meet the standard, and further increases the re-fermentation temperature of the second gas injection unit by increasing the heating temperature of the second heating unit if the temperature of the tail end fermentation reactor is lower than the preset re-fermentation temperature, and further increases the re-fermentation temperature of the tail end fermentation reactor if the temperature of the tail end fermentation reactor is higher than the preset re-fermentation temperature, and the microorganism injection rate is not met by the second gas injection unit.
In particular, the invention sets a plurality of ultrasonic generators on the tail end fermentation reactor, the ultrasonic generators have oscillation and strengthening culture effects on microorganisms in the tail end fermentation reactor, the control machine obtains that the rotation rate of the second gas injection unit exceeds the maximum value of the preset rotation rate of the second gas injection unit, and the control machine promotes the fermentation of the microorganisms in the tail end fermentation reactor by starting the ultrasonic generators and improving the frequency of the ultrasonic generators according to the difference value between the regulated rotation rate and the preset rotation rate of the second gas injection unit.
In particular, in order to prevent the sterilized gas quantity in the air compressor from not reaching the injection quantity into each fermentation reactor, the controller adjusts the temperature conversion frequency of the air compressor according to the gas injection rate of the first gas injection unit and the gas injection rate of the second gas injection unit after the adjustment so as to improve the convection rate in the air compressor, improve the sterilization rate of the gas in the air compressor and ensure that a large quantity of sterilized gas is injected into the head fermentation reactor and the tail fermentation reactor so as to ensure that the sterilized gas quantity produced in the air compressor can meet the injection quantity.
In particular, when the microbial cell density in the head end fermentation reactor exceeds the preset cell density range, and the air compressor is determined to have poor sterilization effect after other site pollution points are discharged, the sterilization efficiency is improved by improving the temperature conversion frequency of the air compressor.
Drawings
FIG. 1 is a schematic diagram of a microbial fermenter for improving degradation efficiency according to an embodiment of the present invention;
FIG. 2 is a standard curve of Bacillus growth in accordance with an embodiment of the present invention;
FIG. 3 is a schematic diagram of a head fermentation reactor according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a tail end fermentation reactor according to an embodiment of the present invention;
fig. 5 is a schematic diagram of the structure of each injection unit according to an embodiment of the invention.
Detailed Description
In order that the objects and advantages of the invention will become more apparent, the invention will be further described with reference to the following examples; it should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are merely for explaining the technical principles of the present invention, and are not intended to limit the scope of the present invention.
Referring to fig. 1, a schematic structure of a microbial fermentation tank for improving degradation efficiency according to an embodiment of the invention includes,
the head end fermentation reactor 1 is provided with a barrel-shaped first tank body, the first tank body is provided with a space for fermenting microorganisms and culture solution wrapped in a membrane, the bottom in the first tank body is provided with a first gas injection unit 12 for providing gas required in the pre-fermentation process, and the outside of the first tank body is provided with a first heating unit 11;
The tail end fermentation reactor 2 is connected with the head end fermentation reactor and is provided with a second tank body for secondary fermentation of the pre-fermented product generated by the head end fermentation reactor, the bottom of the second tank body is provided with a second gas injection unit 22 for secondary fermentation of the pre-fermented product, the outer part of the second tank body is provided with a second heating unit 23, and the tail end fermentation reactor also comprises a guide pipe 24 which extends into the first tank body and is connected with the bottom of the second tank body, and a first pump body 23 for controlling the amount of the pre-fermented product injected into the second tank body;
an air compressor 3, which is provided with two exhaust pipelines, each exhaust pipeline is used for injecting gas into the head fermentation reactor and the tail fermentation reactor respectively, wherein a first exhaust pipeline is provided with a second pump body 31 for controlling the gas injection rate into the head fermentation reactor, a second exhaust pipeline is provided with a third pump body 37 for controlling the gas injection rate into the tail fermentation reactor, the air compressor is internally provided with a sterilizing mechanism for sterilizing the injected gas, two sides of the air compressor are provided with heating mechanisms for providing convection for air in the air compressor, the heating mechanisms are connected with the first heating unit and the second heating unit so as to transfer heat energy of the heating mechanisms to the head fermentation reactor and the tail fermentation reactor, the heating mechanisms comprise a first heat exchanger 32 for controlling the temperature of the first heating pipe, a second heat exchanger 33 for controlling the temperature of the second heating pipe, the first heating pipe is provided with a first three-way valve 34, the second heating pipe is provided with a second three-way valve 36, and the heating mechanism also comprises a reversing valve 35 for mutually reversing the heat energy of the first heating unit and the second heating unit;
The control machine is connected with the head end fermentation reactor, the tail end fermentation reactor and the air compressor and is used for injecting the pre-fermentation liquid into the tail end fermentation reactor when the growth vigor of microorganisms in the head end fermentation reactor is in the logarithmic phase, and adjusting the temperature conversion frequency of the heating mechanism according to the growth rate of the microorganisms in the tail end fermentation reactor so as to improve the degradation efficiency of the microorganisms in the culture liquid.
In use, the attachment of microorganism is fermented in the head end fermentation reactor to the logarithmic growth phase of microorganism, then is injected into the tail end fermentation reactor for re-fermentation, in the fermentation process of the head end fermentation reactor and the tail end fermentation reactor, the air in the air compressor is respectively injected into the head end fermentation reactor and the tail end fermentation reactor through each exhaust pipeline after being sterilized, and the fermentation liquor in each fermentation reactor is stirred and supplied with air, so as to improve the sterilization efficiency of the air in the air compressor, the first heat exchanger of the heating mechanism heats the gas at one side of the air compressor through the heating pipe arranged at one side of the air compressor, the first three-way valve opens the valve passing through the first heating unit, the liquid heated at one side of the air compressor is injected into the first heating unit, the heated liquid is used for providing heat for the head fermentation reactor, energy resources are fully utilized, resource waste is avoided, at the moment, the second heat exchanger cools the gas at the other side of the air compressor through the heating pipe arranged at the other side of the air compressor, the second three-way valve opens the valve passing through the second recovery pipeline, the cooled liquid is recovered, after a period of time, the first heat exchanger opens the valve passing through the first recovery pipeline through the first three-way valve for cooling the gas at one side of the air compressor through the heating pipe arranged at one side of the air compressor, and recovering the liquid cooled by the gas at one side of the air compressor, heating the gas at the other side of the air compressor by the second heat exchanger through a heating pipe arranged at the other side of the air compressor, and opening a valve passing through a second heating unit by a second three-way valve to supply the heated liquid to the tail end fermentation reactor for heat.
Specifically, the invention attaches microorganisms to a membrane, can make the attached microorganisms gather and grow in a culture solution, and are separated from the culture solution, and utilize nutrient substances of the culture solution to reproduce, and meanwhile, the attached microorganisms are gradually separated from the attached substances in the growth and reproduction process, so that the microorganisms grow orderly, and meanwhile, the attached microorganisms are ensured to remain for recycling, so that the use time of the microorganisms can be prolonged while fermentation is realized.
Specifically, a streptococcus thermophilus starter is produced by using a microorganism fermentation tank as an example, and the streptococcus thermophilus starter is cultured in a culture solution at 42 ℃ for 10 hours to form activated streptococcus thermophilus, wherein attachments for growth of the streptococcus thermophilus are arranged in the culture solution, after activation, the attachments attached with the streptococcus thermophilus are put into a head end fermentation reactor, nutrients of the culture solution in the head end fermentation reactor are utilized for propagation, when the growth stage of microorganisms in a film in the head end fermentation reactor is a logarithmic phase, a pre-fermentation solution in the head end fermentation reactor is injected into a tail end fermentation reactor and mixed with the culture solution in the tail end fermentation reactor for fermentation, and then the acidophilus starter is prepared after 5-6 hours, wherein the activated streptococcus thermophilus culture solution comprises lactose 5.0g, soyase peptone 5.0g, tryptone 6.0g, beef extract 6.0g, yeast powder 2.5 g, magnesium sulfate 0.5g, beta-glycerophosphate 20.0g, distilled water is quantitatively added to 1000ml, and the attachments are sterilized by using the yeast extract and distilled water to prepare the acidophilus starter after the activated streptococcus thermophilus starter, the activated streptococcus thermophilus starter is sterilized by the method comprises the steps of 5.0g, the soybean peptone 5.0g, the tryptone is added into the yeast extract, and the distilled water is quantitatively sterilized to obtain the acidophilic starter.
Specifically, the embodiment of the invention adopts a microbial fermentation tank to produce a bacillus starter, the bacillus is inoculated into a culture medium for activation, the activated bacillus is mixed with a culture solution, attachments for the growth of the bacillus are arranged in the culture solution, the attachments with the bacillus are put into a head fermentation reactor for culturing for 4 hours, microorganisms in the head fermentation reactor are cultured to a logarithmic growth phase and then are injected into a tail reaction fermentation phase for secondary fermentation, and the bacillus starter is prepared through 6 hours of fermentation, wherein the activation culture medium comprises 20g of whole milk powder, 10g of agar, 0.05g of manganese sulfate heptahydrate, 20g of calcium carbonate, 5.0g of sodium acetate, 2.0g of diamine citrate, 2.0g of dipotassium phosphate, 0.02g of magnesium sulfate heptahydrate, 1.0g of tween, distilled water is quantified to 1000ml, and the culture solution of the head fermentation reactor comprises 25g of whole milk powder, 0.05g of manganese sulfate heptahydrate, 5.0g of sodium acetate, 2.0g of diamine citrate, 2.0g of dipotassium phosphate, 2.0g of magnesium sulfate, 1.02 g of tween, and other nutrient solution, and the culture solution is distilled water is quantitatively sterilized, and the culture solution is prepared into a yoghurt.
Specifically, the embodiment of the invention adopts a microorganism fermentation tank which can also be applied to fermenting organic fertilizer, decomposed kitchen waste and the like, takes fermenting organic fertilizer as an example, activates microbial inoculum and adheres to attachments, puts the attachments into a head fermentation reactor, takes a culture medium in the head fermentation reactor as a culture solution suitable for growth of the microbial inoculum, puts the microbial inoculum into a tail fermentation reactor when the cell density change rate of the microbial inoculum in the head fermentation reactor reaches a preset standard, and puts the microbial inoculum into the tail fermentation reactor as organic material to be fermented, such as feces or straws and the like.
Wherein the control machine compares the microbial cell density in the head end fermentation reactor obtained in the preset culture time with the cell density of a microbial growth standard curve to judge the microbial growth rate, when the growth rate does not meet the standard, the control machine judges whether to adjust the rotation rate of the first gas injection unit and the gas injection amount according to the temperature of the head end fermentation reactor or the dispersion degree of the microbes, wherein the control machine obtains the microbial cell density change rate P in the head end fermentation reactor in the preset culture time and the microbial cell density change rate P in the logarithmic growth phase of the microbial growth standard curve to judge the growth rate of the microbes,
When P is less than P-delta P, the controller judges that the growth rate of microorganisms in the head end fermentation reactor does not meet the standard, and the growth period is not in the logarithmic growth phase;
when P-delta P is less than or equal to P and less than or equal to P+ [ delta ] P, the control machine judges that the growth rate of microorganisms in the head end fermentation reactor meets the standard, and the growth period is in the logarithmic growth phase;
when P is more than P+ [ delta ] P, the controller judges that the microorganism growth rate in the head end fermentation reactor does not meet the standard;
wherein Δp is the error value of the cell density change rate preset by the controller.
Specifically, the method is not limited in the measurement of the cell density of the microorganism in the head-end fermentation reactor, the concentration of the secondary metabolite in the fermentation liquor in the head-end fermentation reactor can be used for representing the cell density of the microorganism, the change rate of the cell density of the microorganism can be further determined by measuring the number of the cells of the microorganism in the head-end fermentation reactor, a microorganism growth standard curve is drawn according to the cell density of the microorganism to be cultivated in each cultivation time in the cultivation process, bacillus is taken as an example, referring to fig. 2, which is a bacillus growth standard curve schematic diagram of the method, the change rate P of the cell density in the logarithmic growth phase in the bacillus standard curve is 1.7, the spore content ρ1 of the secondary metabolite in the bacillus fermentation process is the index of the cell density, the spore content ρ1 of the secondary metabolite in the first preset cultivation time point bacillus is the cell density of the first preset cultivation time, the spore content ρ2 of the secondary metabolite in the second preset cultivation time point bacillus is the cell density of the second preset time point, and the spore content ρ3 of the spore content of the third preset cultivation time point ρ3- ρ2 of the spore content of the secondary metabolite in the bacillus is the cell density of the third preset time point ρ2)/(2 of the cell density of the microorganism, = (ρ2- ρ2); when the cell number of bacillus in the bacillus fermentation process is used as an index of the cell density, the cell number ρ1 in the quantitative sampling of the bacillus at a first preset culture time point in the head fermentation reactor is the cell density at the first preset culture time point, the cell number ρ2 in the quantitative sampling of the bacillus at a second preset culture time point is the cell density at the second preset culture time point, the cell number ρ3 in the quantitative sampling of the bacillus at a third preset culture time point is the cell density at the third preset culture time point, and the change rate p= (ρ3- ρ2)/(ρ2- ρ1) of the cell density of the microorganism is set.
Specifically, the invention evaluates the growth rate of the microorganism in the head-end fermentation reactor according to the comparison of the cell density change rate of the microorganism in the head-end fermentation reactor and the cell density change rate in the growth log phase in a standard curve, and judges whether the microorganism is in the growth log phase, wherein if the microorganism cell density change rate is smaller than the error range of the cell density change rate in the growth log phase of the standard curve, the microorganism growth rate in the head-end fermentation reactor is not in accordance with the standard, the lower standard value proves that the microorganism growth rate does not reach the logarithmic growth phase, the efficiency of the current head-end fermentation reactor is too low, if the microorganism cell density change amount is larger than the error range of the cell density change rate in the growth log phase of the standard curve, the current growth rate is too fast, the condition that foreign bacteria possibly exist is indicated, the purity of the microorganism is detected, and if the microorganism cell density change rate is in the error range of the cell density change rate in the growth log phase of the standard curve, the microorganism growth rate in the head-end fermentation reactor is in accordance with the standard curve, the growth cycle is in the logarithmic growth phase, the microorganism cell density change rate is injected into the tail-end fermentation broth in the logarithmic growth phase for fermentation.
The control machine increases the first temperature of the first heat exchanger of the heating mechanism according to the temperature conversion frequency F of the first heat exchanger and the second heat exchanger of the heating mechanism under the first condition so as to increase the temperature of the head fermentation reactor, wherein when F is less than or equal to F, the control machine increases the first temperature w1 to w11 of the first heat exchanger by adopting a first adjustment parameter k1, and w11=w1×k1 is set; when F is more than F, the control machine opens a reversing valve connected between the first heating unit and the second heating unit, and transmits heat of the second heating unit to the first heating unit;
the first condition is that the fermentation temperature of the head-end fermentation reactor is lower than a preset fermentation temperature, the growth period of microorganisms in the head-end fermentation reactor is not in a logarithmic growth phase, the controller presets a temperature conversion frequency F, k1=k0× (1+ (F-F)/F0), wherein k0 is a preset regulation parameter standard value, and F0 is a preset temperature conversion frequency standard value.
Specifically, under the condition that the growth period of microorganisms in the head-end fermentation reactor does not reach the growth log period within the preset time, and the temperature of the head-end fermentation reactor is detected to be too low, the first temperature of the first heat exchanger is corrected according to the temperature conversion frequency of the first heat exchanger and the second heat exchanger, so that the problem that the temperature of the head-end fermentation reactor is too low to influence the growth rate of microorganisms cultured by the head-end fermentation reactor due to the too fast temperature conversion frequency is avoided, wherein the temperature conversion frequency obtained by the controller is lower than the preset temperature conversion frequency, the reason that the temperature of the second heating unit is too low is not in a temperature holding period caused by the temperature conversion frequency, the temperature of the second heating unit is too low, the temperature of the first heat exchanger is too low, the controller selects the largest adjusting parameter to increase the first temperature of the first heat exchanger, the temperature of the head-end fermentation reactor is further improved, the current reason that the temperature of the head-end fermentation reactor cannot reach the standard due to the too fast temperature conversion frequency is shortened when the temperature conversion frequency obtained by the air compressor exceeds the preset temperature conversion frequency, the first heating unit is connected to the first reversing unit, the heating unit is connected to the first heating unit, and the first reversing unit is heated, and the heating unit is heated by the first reversing unit is connected to the first heating unit.
Specifically, the temperature change frequency of the air compressor is not limited, and needs to be specifically set according to the amount of gas sterilized once in the air compressor, and the embodiment of the invention provides a preferred embodiment, when bacillus is fermented, the optimal temperature in the head fermentation reactor is 30 ℃, the optimal temperature in the tail fermentation reactor is 35 ℃, the high temperature of the first heat exchanger, namely the first temperature of the first heat exchanger, is set to 35-40 ℃, the low temperature of the first heat exchanger, namely the second temperature of the first heat exchanger, is set to 25-30 ℃, the high temperature of the second heat exchanger, namely the first temperature of the second heat exchanger, is set to 40-45 ℃, the low temperature of the second heat exchanger, namely the second temperature of the second heat exchanger, is set to 25-30 ℃, and the exchange time of the high temperature and the low temperature of each heat exchanger in the heating mechanism is 5-8min, namely the temperature change frequency of the first heat exchanger is set to 35-40 ℃, and the air injection amount of 30ml per unit time can be provided for each fermentation reactor of 1L volume.
The control machine adjusts the rotation rate and the gas injection rate of the first gas injection unit according to the microbial dispersion degree Y in the head end fermentation reactor under a second condition, wherein when Y is less than or equal to Y, the control machine improves the gas injection rate vs of the first gas injection unit to vs1, and vs1 = vs× (1+ (Y-Y)/Y) is set; when Y is more than Y, the controller increases the rotation rates cs to cs1 of the first gas injection unit, and cs1=cs× (1+ (Y-Y)/Y) is set;
The second condition is that the fermentation temperature of the head end fermentation reactor is higher than a preset pre-fermentation temperature, the growth period of microorganisms in the head end fermentation reactor is not in the logarithmic growth phase, and the controller presets a dispersion degree standard value Y.
Specifically, in the invention, when the fermentation temperature in the head end fermentation reactor is higher than the preset pre-fermentation temperature, the reason that the growth period of the microorganisms in the head end fermentation reactor does not reach the logarithmic growth period within the preset time is not the fermentation temperature, but the reason that the microorganisms in the head end fermentation reactor are unevenly mixed with the culture solution or the gas injection amount is insufficient is that the control machine obtains the dispersion degree of the microorganisms in the head end fermentation reactor and compares the dispersion degree with the preset dispersion degree, and the dispersion degree accords with the standard, namely the dispersion degree of the microorganisms in the head end fermentation reactor is smaller than or equal to the preset dispersion degree, the gas injection amount in the head end fermentation reactor is regulated and controlled by improving the gas injection rate of the first gas injection unit, and when the dispersion degree does not accord with the standard, the dispersion degree of the microorganisms in the head end fermentation reactor accords with the standard by improving the rotation rate of the first gas injection unit.
Specifically, the embodiment of the invention obtains the cell density of each point in the head end fermentation reactor by sampling each point in the head end fermentation reactor and determines the dispersion degree of microorganisms according to a variance formula, and more specifically, the embodiment of the invention obtains the cell density of each point in the head end fermentation reactor as 6.2 multiplied by 10 5 CFU/ml、5.9×10 5 CFU/ml、7.1×10 5 CFU/ml、6.0×10 5 CFU/ml、5.8×10 5 CFU/ml, the degree of dispersion of the microorganism in real time ((6.2-6.2) was obtained 2 +（5.9-6.2） 2 +（7.1-6.2） 2 +（6.0-6.2） 2 +（5.8-6.2） 2 ) 5=0.22, which is less than the preset dispersion level of 0.8, i.e. meets the standard.
Referring to fig. 3, which is a schematic structural diagram of a head-end fermentation reactor according to an embodiment of the present invention, the head-end fermentation reactor includes a microorganism injection unit, the microorganism injection unit includes a driver 13 for controlling a rotation rate of an injector 14, the controller presets a first air injection unit rotation rate maximum value csmax, the adjusted rotation rate cs1 of the first air injection unit is greater than the preset first air injection unit rotation rate maximum value csmax, the controller determines that the rotation rate of the first air injection unit is set to csmax, and increases a rotation rate vb of a next microorganism injection to vb1, and vb1=vb× (1+ (cs 1-csmax)/csmax) is set.
With continued reference to fig. 3, the head-end fermentation reactor is provided with a plurality of sampling ports for detecting the microbial cell density, wherein the first sampling port 15, the second sampling port 16 and the third sampling port 17 are respectively used for detecting the microbial cell density of each point of the head-end fermentation reactor.
Specifically, the microbial injection unit capable of controlling the injection rotation speed is arranged at the top of the head end fermentation reactor, and when the rotation speed of the first air injection unit reaches the maximum, the control machine adjusts the rotation speed of the first air injection unit to the preset maximum value and controls the rotation speed of the microbial injection unit to be improved so as to improve the initial dispersion degree of microorganisms in the next pre-fermentation process in order to avoid microbial cell breakage caused by the overlarge rotation speed of the first air injection unit.
After the pre-fermented product of the head end fermentation reactor is injected into the tail end fermentation reactor, the microbial growth rate of the tail end fermentation reactor is detected through the preset re-fermentation time, the control machine compares the obtained microbial growth rate H of the tail end fermentation reactor with the preset growth rate H, if the microbial growth rate of the tail end fermentation reactor is larger than the preset growth rate, the control machine does not adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit, and if the microbial growth rate of the tail end fermentation reactor is smaller than the preset growth rate, the control machine judges whether to adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit according to the temperature of the tail end fermentation reactor.
Wherein when the microorganism growth rate of the tail end fermentation reactor is smaller than the preset growth rate, the control machine obtains the temperature D of the tail end fermentation reactor and compares the temperature D with the preset re-fermentation temperature D, and judges whether to adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit,
when D is less than or equal to D, the controller judges that the first temperature w2 of the second heat exchanger is increased to w21, and w21=w2× (1+ (D-D)/D) is set;
When D > D, the controller increases the rotation rate cw to cw1 of the second gas injection unit, sets cw1=cw× (1+ (D-D)/D), and simultaneously increases the gas injection rate vw to vw1 of the second gas injection unit, sets vw1=vw× (1+ (D-D)/D).
Specifically, the invention detects the microorganism growth rate of the tail end fermentation reactor after fermentation, compares the obtained microorganism growth rate of the tail end fermentation reactor with a preset growth rate, judges whether the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit are regulated according to the temperature of the tail end fermentation reactor, wherein the microorganism growth rate of the tail end fermentation reactor is larger than the preset growth rate, judges that the microorganism growth rate of the current tail end fermentation reactor meets the standard, when the microorganism growth rate of the tail end fermentation reactor is smaller than the preset growth rate, judges that the microorganism growth rate of the current tail end fermentation reactor does not meet the standard, the controller compares the temperature of the tail end fermentation reactor with the preset re-fermentation temperature to determine the reason that the microorganism growth rate of the tail end fermentation reactor does not meet the standard, and if the temperature of the tail end fermentation reactor is lower than the preset re-fermentation temperature, the temperature of the second heat exchanger is increased to further increase the re-fermentation temperature of the second heat unit, and if the temperature of the tail end fermentation reactor is higher than the preset re-fermentation temperature, and the microorganism rate is not fed into the fermentation reactor by the second gas injection unit, so that the microorganism growth rate is not met by the microbial injection unit.
Referring to fig. 4, which is a schematic structural diagram of a tail end fermentation reactor according to an embodiment of the present invention, at least one ultrasonic generator is disposed on an outer wall of the tail end fermentation reactor, the controller presets a rotation rate cwmax of the second gas injection unit, and when the adjusted rotation rate cw1 of the second gas injection unit is greater than a preset maximum cwmax of the rotation rate of the second gas injection unit, the controller sets the rotation rate of the second gas injection unit to cwmax, and starts the ultrasonic generator, adjusts a frequency E of the ultrasonic generator to E1, and sets e1=e× (1+ (cw 1-cwmax)/cwmax).
With continued reference to fig. 4, in the embodiment of the present invention, 5 ultrasonic generators are disposed on the outer wall of the tail end fermentation reactor at intervals, and include a first ultrasonic generator 25, a second ultrasonic generator 26, a third ultrasonic generator 27, a fourth ultrasonic generator 28, and a fifth ultrasonic generator 29, where the number of ultrasonic generators is not limited, so long as they can provide ultrasonic waves for the microorganisms to be fermented in the tail end fermentation reactor, and the frequency of the ultrasonic generators is 20kHz.
Specifically, the ultrasonic generators are arranged on the tail end fermentation reactor, have oscillation and enhanced culture effects on microorganisms in the tail end fermentation reactor, the control machine obtains the maximum value that the rotation rate of the second gas injection unit exceeds the preset rotation rate of the second gas injection unit, and the control machine promotes fermentation of the microorganisms in the tail end fermentation reactor by starting the ultrasonic generators and increasing the frequency of the ultrasonic generators according to the difference value between the regulated rotation rate and the preset rotation rate of the second gas injection unit.
The controller shortens the temperature change frequencies f0 to f1 of the air compressor according to the adjusted injection rate vs1 of the first air injection unit and the adjusted injection rate vw1 of the second air injection unit, and sets f1=f× (1-0.5× (vs 1-vs)/vs×0.8× (vw 1-vw)/vw).
Specifically, in order to avoid that the gas amount sterilized in the air compressor cannot reach the injection amount into each fermentation reactor, the controller adjusts the temperature conversion frequency of the air compressor according to the gas injection rate of the first gas injection unit and the gas injection rate of the second gas injection unit after being acquired and adjusted, so as to improve the convection rate in the air compressor, improve the gas sterilization rate in the air compressor, ensure that a large amount of sterilized gas is injected into the head fermentation reactor and the tail fermentation reactor, and ensure that the gas amount qualified by sterilization produced in the air compressor can meet the injection amount.
The controller increases the temperature conversion frequency fq to fq1 of the air compressor under a third condition, fq1=fqx1.25 is set, the third condition is P > P+ [ delta ] P, and the controller judges that the sterilization of the air compressor does not meet the standard, and q=0, 1.
Specifically, when the microbial cell density in the head end fermentation reactor exceeds the preset cell density range, and when the air compressor is determined to be poor in sterilization effect after other site pollution points are discharged, the sterilization efficiency is improved by improving the temperature conversion frequency of the air compressor.
Referring to fig. 5, which is a schematic structural diagram of each injection unit according to the embodiment of the present invention, the injection unit includes a first gear 43, a second gear 42 meshed with the first gear, and an air injection pipe 41 disposed on the second gear, in use, a motor disposed below the first gear controls the first gear to rotate so as to drive the second gear to rotate along the first gear, so as to realize air injection of the air injection pipe at each position in each fermentation reactor. The first gas injection unit and the second gas injection unit are similar in structure, and gears with different sizes are arranged according to the fermentation spaces of the head fermentation reactor and the tail fermentation reactor.
Thus far, the technical solution of the present invention has been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of protection of the present invention is not limited to these specific embodiments. Equivalent modifications and substitutions for related technical features may be made by those skilled in the art without departing from the principles of the present invention, and such modifications and substitutions will be within the scope of the present invention.
Claims (7)
1. A microbial fermentation tank for improving degradation efficiency, comprising:
The head end fermentation reactor is provided with a barrel-shaped first tank body, the first tank body is provided with a space for fermenting microorganisms and culture solution wrapped in a membrane, the bottom in the first tank body is provided with a first gas injection unit for providing gas required in the pre-fermentation process, and the outside of the first tank body is provided with a first heating unit;
the tail end fermentation reactor is connected with the head end fermentation reactor and is provided with a second tank body for secondary fermentation of the pre-fermented product generated by the head end fermentation reactor, the bottom of the second tank body is provided with a second gas injection unit for secondary fermentation of the pre-fermented product, and the outer part of the second tank body is provided with a second heating unit;
the air compressor is provided with two exhaust pipelines, each exhaust pipeline respectively injects gas into the head end fermentation reactor and the tail end fermentation reactor, a sterilization mechanism for sterilizing the injected gas is arranged in the air compressor, heating mechanisms for providing convection for air in the air compressor are arranged on two sides of the air compressor, and the heating mechanisms are connected with the first heating unit and the second heating unit so as to transfer heat energy of the heating mechanisms to the head end fermentation reactor and the tail end fermentation reactor; the heating mechanism comprises a first heat exchanger for controlling the temperature of a first heating pipe and a second heat exchanger for controlling the temperature of a second heating pipe, wherein a first three-way valve is arranged on the first heating pipe, a second three-way valve is arranged on the second heating pipe, and the heating mechanism further comprises a reversing valve for mutually converting the heat of the first heating unit and the heat of the second heating unit;
The control machine is connected with the head end fermentation reactor, the tail end fermentation reactor and the air compressor and is used for injecting the pre-fermentation liquid into the tail end fermentation reactor when the growth vigor of microorganisms in the head end fermentation reactor is in the logarithmic phase, and adjusting the temperature conversion frequency of the heating mechanism according to the growth rate of the microorganisms in the tail end fermentation reactor so as to improve the degradation efficiency of the microorganisms in the culture liquid; after the microorganisms attached to the attachments are fermented in the head fermentation reactor to the logarithmic growth phase of the microorganisms, the microorganisms are injected into the tail fermentation reactor for re-fermentation, in the fermentation process of the head fermentation reactor and the tail fermentation reactor, air in the air compressors is respectively injected into the head fermentation reactor and the tail fermentation reactor through the exhaust pipelines after being sterilized, fermentation liquid in each fermentation reactor is stirred and supplied, in order to improve the sterilization efficiency of the air in the air compressors, the heating mechanism is arranged on the air compressors, the air on the air compressors is heated and cooled through the heat exchanger of the heating mechanism, the convection of the air in the air compressors is realized, the flow of the air is improved, the sterilization efficiency of the air is further improved, the first heat exchanger of the heating mechanism heats the air on one side of the air compressors through the first heating pipe arranged on one side of the air compressor, the first three-way valve is opened through the valve of the first heating unit, the liquid heated on one side of the air compressor is injected into the first heating unit, the used liquid heated up to the head fermentation reactor provides heat for the air compressor, the second heat exchanger is cooled through the first three-way valve arranged on the other side of the air compressor, the air compressor is cooled through the first three-way valve arranged on the other side of the first heat exchanger, the air compressor is cooled through the valve is opened, the air recovery valve is cooled through the first three-way valve arranged on the air valve arranged on the other side of the air compressor, the air compressor is cooled through the valve is cooled down, the air recovery valve is cooled through the first valve arranged on the air valve is cooled through the first valve arranged on the side after the first valve is opened, the second heat exchanger heats the gas at the other side of the air compressor through a second heating pipe arranged at the other side of the air compressor, the second three-way valve opens a valve passing through the second heating unit, and the heated liquid is supplied to the tail end fermentation reactor for heat;
The control machine compares the microbial cell density in the head end fermentation reactor obtained in the preset culture time with the cell density of a microbial growth standard curve to judge the microbial growth rate, when the growth rate does not accord with the standard, the control machine judges whether to adjust the rotation rate of the first gas injection unit and the gas injection amount according to the temperature of the head end fermentation reactor or the dispersion degree of the microbes, wherein the control machine obtains the microbial cell density change rate P in the head end fermentation reactor in the preset culture time and the microbial cell density change rate P in the logarithmic growth phase of the microbial growth standard curve to judge the growth rate of the microbes,
when P is less than P-delta P, the controller judges that the growth rate of microorganisms in the head end fermentation reactor does not meet the standard, and the growth period is not in the logarithmic growth phase;
when P-delta P is less than or equal to P and less than or equal to P+ [ delta ] P, the control machine judges that the growth rate of microorganisms in the head end fermentation reactor meets the standard, and the growth period is in the logarithmic growth phase;
when P is more than P+ [ delta ] P, the controller judges that the microorganism growth rate in the head end fermentation reactor does not meet the standard;
Wherein Deltap is a cell density change rate error value preset by the control machine;
the control machine increases the first temperature of the first heat exchanger of the heating mechanism according to the temperature conversion frequency F of the first heat exchanger and the second heat exchanger of the heating mechanism under the first condition so as to increase the temperature of the head fermentation reactor, wherein when F is less than or equal to F, the control machine increases the first temperature w1 to w11 of the first heat exchanger by adopting a first adjustment parameter k1, and w11=w1×k1 is set; when F is more than F, the control machine opens a reversing valve connected between the first heating unit and the second heating unit, and transmits heat of the second heating unit to the first heating unit;
the first condition is that the fermentation temperature of the head-end fermentation reactor is lower than a preset fermentation temperature, the growth period of microorganisms in the head-end fermentation reactor is not in a logarithmic growth phase, the controller presets a temperature conversion frequency F, k1=k0× (1+ (F-F)/F0), wherein k0 is a preset regulation parameter standard value, and F0 is a preset temperature conversion frequency standard value;
the control machine adjusts the rotation rate and the gas injection rate of the first gas injection unit according to the microbial dispersion degree Y in the head end fermentation reactor under a second condition, wherein when Y is less than or equal to Y, the control machine improves the gas injection rate vs of the first gas injection unit to vs1, and vs1 = vs× (1+ (Y-Y)/Y) is set; when Y is more than Y, the controller increases the rotation rates cs to cs1 of the first gas injection unit, and cs1=cs× (1+ (Y-Y)/Y) is set;
The second condition is that the fermentation temperature of the head end fermentation reactor is higher than a preset pre-fermentation temperature, the growth period of microorganisms in the head end fermentation reactor is not in the logarithmic growth phase, and the controller presets a dispersion degree standard value Y.
2. The microbial fermentation cylinder for improving degradation efficiency according to claim 1, wherein the head end fermentation reactor comprises a microbial injection unit including a driver for controlling the rotational rate of the injector, the controller presets a first air injection unit rotational rate maximum value csmax, the adjusted first air injection unit rotational rate cs1 is greater than the preset first air injection unit rotational rate maximum value csmax, the controller decides to set the first air injection unit rotational rate to csmax while increasing the rotational rate vb at the next microbial injection to vb1, and vb1=vb× (1+ (cs 1-csmax)/csmax) is set.
3. The microbial fermentation tank for improving degradation efficiency according to claim 2, wherein the pre-fermented product of the head fermentation reactor is injected into the tail fermentation reactor for a preset re-fermentation time, the microbial growth rate of the tail fermentation reactor is detected, the control machine compares the obtained microbial growth rate H of the tail fermentation reactor with the preset growth rate H, if the microbial growth rate H of the tail fermentation reactor is greater than the preset growth rate, the control machine does not adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit, and if the microbial growth rate of the tail fermentation reactor is less than the preset growth rate, the control machine determines whether to adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit according to the temperature of the tail fermentation reactor.
4. The microbial fermentation cylinder for improving degradation efficiency according to claim 3, wherein when the microbial growth rate of the tail end fermentation reactor is smaller than the preset growth rate, the control machine obtains the temperature D of the tail end fermentation reactor and compares the temperature D with the preset re-fermentation temperature D to judge whether to adjust the gas injection rate of the second gas injection unit and the rotation rate of the second gas injection unit,
when D is less than or equal to D, the controller judges that the first temperature w2 of the second heat exchanger is increased to w21, and w21=w2× (1+ (D-D)/D) is set;
when D > D, the controller increases the rotation rate cw to cw1 of the second gas injection unit, sets cw1=cw× (1+ (D-D)/D), and simultaneously increases the gas injection rate vw to vw1 of the second gas injection unit, sets vw1=vw× (1+ (D-D)/D).
5. The microbial fermentation vessel for improving degradation efficiency according to claim 4, wherein at least one ultrasonic generator for promoting microbial fermentation is provided on the outer wall of the tail fermentation reactor, the controller presets the second gas injection unit rotation rate cwmax, and when the adjusted second gas injection unit rotation rate cw1 is greater than the preset second gas injection unit rotation rate maximum cwmax, the controller sets the second gas injection unit rotation rate to cwmax, and activates the ultrasonic generator, adjusts the frequency E of the ultrasonic generator to E1, and sets e1=e× (1+ (cw 1-cwmax)/cwmax).
6. The microbial fermentation cylinder for improving degradation efficiency according to claim 5, wherein the controller shortens the temperature change frequencies f0 to f1 of the air compressor according to the adjusted injection rate vs1 of the first gas injection unit and the adjusted injection rate vw1 of the second gas injection unit, and sets f1=f× (1-0.5× (vs 1-vs)/vs×0.8× (vw 1-vw)/vw).
7. The microbial fermenter according to claim 6, wherein the controller increases the temperature conversion frequency fq to fq1 of the air compressor under a third condition, fq1=fq×1.25, q=0, 1, and the third condition is P > p++Δp, and the controller determines that the air compressor sterilization does not meet the standard.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202211569845.3A CN115627215B (en) | 2022-12-08 | 2022-12-08 | Microbial fermentation tank capable of improving degradation efficiency |
Applications Claiming Priority (1)
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