CN115624996B - Device for biological experiment for high-grade biological safety laboratory - Google Patents

Device for biological experiment for high-grade biological safety laboratory Download PDF

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Publication number
CN115624996B
CN115624996B CN202211323362.5A CN202211323362A CN115624996B CN 115624996 B CN115624996 B CN 115624996B CN 202211323362 A CN202211323362 A CN 202211323362A CN 115624996 B CN115624996 B CN 115624996B
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water bath
disc
water
fixed disc
movable
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CN115624996A (en
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章嫡妮
董姗姗
于赐刚
张振华
郭晓平
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Nanjing Institute of Environmental Sciences MEE
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Nanjing Institute of Environmental Sciences MEE
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/02Water baths; Sand baths; Air baths
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Abstract

The invention discloses a device for biological experiments in a high-grade biological safety laboratory, which comprises a water bath kettle, wherein an electric heating piece and an inner kettle are arranged in the water bath kettle, the water bath kettle is of a sealed structure, a sealing cover is arranged at the top of the water bath kettle, the device also comprises a pressure sensor, an air exhaust piece and a controller, the pressure sensor is arranged in the water bath kettle, the air exhaust piece is communicated with the interior of the water bath kettle through a pipeline, and the controller is configured as follows: when the electric heating piece is heated, the boiling pressure value corresponding to the water just boiling at the preset heating temperature is matched according to the preset heating temperature, the actual pressure value in the water bath is obtained through the pressure sensor, the air pumping piece is controlled to pump air, the actual pressure value in the water bath is always equal to the boiling pressure value, and the water is boiled at the preset heating temperature. The device for the biological experiment has the advantages that the self-protection performance, the heating temperature accuracy, the controllability, the heating temperature range and the like all meet the use requirements of a high-grade biological safety laboratory when the device is heated in a water bath.

Description

Device for biological experiment for high-grade biological safety laboratory
Technical Field
The invention relates to the technical field of biological laboratory equipment, in particular to a device for biological experiments in a high-grade biological safety laboratory.
Background
A biosafety laboratory (biosafety laboratory), also called a biosafety containment laboratory (biosafety containment for laboratories), is a biosafety laboratory and an animal laboratory which can avoid or control the harm of operated harmful biological factors through protective barriers and management measures, and achieve biosafety requirements. The biological safety laboratory is divided into a first-level (BSL-1), a second-level (BSL-2), a third-level (BSL-3) and a fourth-level (BSL-4) according to the biological safety protection level of the laboratory; ABSL-1, ABSL-2, ABSL-3 and ABSL-4 respectively represent the biosafety level of laboratories engaged in live animal operations. The high-grade biosafety laboratory is a laboratory with three-grade and four-grade biosafety protection grades and is used for carrying out scientific research on high-pathogenic microorganisms with 3-grade and 4-grade risk degrees (nationally recognized as first-class and second-class pathogenic microorganisms) internationally. In high-grade biosafety laboratories, dangerous and unknown pathogens need to be handled and may cause pathogens to be transmitted via aerosols or pose a high personal risk, such as the new coronaviruses currently in circulation.
Therefore, the high-grade biosafety laboratory also puts more strict requirements on the self-protection performance of the device for the biological experiment on the basis of implementing two-stage isolation, such as the most common water bath heating experimental device in the high-grade biosafety laboratory. The existing water bath heating experimental device needs to freely discharge water vapor and heated air for safe pressure relief in the heating process, so that the existing water bath heating experimental device is in an open design, but at the same time, in the heating process, heated biological substances are likely to volatilize, and the volatilized substances are likely to bring harm to cause a protection leak, so that the existing water bath heating experimental device cannot be applied to a high-grade biological safety laboratory; in addition, the existing water bath heating experimental device has lower control accuracy on the heating temperature, the water temperature is higher or lower and cannot be adjusted back quickly, and meanwhile, the heating temperature below 100 ℃ can only be provided, which obviously hardly meets the daily use requirement of a high-grade biological safety laboratory.
Disclosure of Invention
The invention aims to provide a device for biological experiments in a high-grade biological safety laboratory, which solves the problem that the safety protection performance, the heating temperature accuracy, the heating temperature range and the like of the existing water bath heating experimental device in the high-grade biological safety laboratory cannot meet the requirements of the laboratory.
The invention realizes the purpose through the following technical scheme:
the utility model provides a device for biological experiments for high-grade biosafety laboratory, the device is including the water bath that is used for holding water, be equipped with the interior pot that is used for the electric heat piece of heating and is used for placing object article in the water bath, the water bath is sealed structure, and the top of water bath is equipped with the sealed lid that can open and shut, and the device still includes pressure sensor, takes out gas spare, controller, inside pressure sensor locates the water bath, the input of taking out gas spare passes through pipeline and the inside intercommunication of water bath, the controller is configured to: when the electric heating piece is heated, the boiling pressure value corresponding to the fact that water just boils at the preset heating temperature is matched according to the preset heating temperature, the actual pressure value in the water bath is obtained through the pressure sensor, the air pumping piece is controlled to conduct air pumping, the actual pressure value in the water bath is enabled to be equal to the boiling pressure value all the time, and therefore the water is enabled to boil at the preset heating temperature all the time.
The further improvement is that the preset heating temperature is 40-134 ℃, and the corresponding boiling pressure value is 7.4-304.0kPa.
The improved MPPS disinfection device is characterized in that the output end of the air pumping part is connected with a purification mechanism, a filter cavity and a disinfection cavity are formed between the input end and the output end of the purification mechanism, an ULPA filter is arranged in the filter cavity, a UV disinfection light source is arranged in the disinfection cavity, and an MPPS leakage detection port is arranged at the output end of the purification mechanism.
The improved structure is characterized in that the output end of the purifying mechanism is connected with a condensing pipe through a pipeline, the condensing pipe is bent in an S shape and extends downwards, and the bottom end of the condensing pipe is communicated with the interior of the water bath through a backflow mechanism.
The further improvement is that a three-way valve is arranged on a connecting pipeline of the purification mechanism and the condensation pipe, one interface of the three-way valve is emptied, and the three-way valve is controlled to enable the purification mechanism to be communicated with the condensation pipe or with the outside air.
The backflow mechanism comprises a cylinder, wherein an input end of the cylinder is communicated with a condensing pipe, an output end of the cylinder is communicated with a water bath, a fixed disc and a movable disc are sequentially intercepted from the input end to the output end in the cylinder, and rotatable opening and closing valve plates are arranged on the fixed disc and the movable disc respectively;
the movable disk is connected with a driving piece for driving the movable disk to move towards or away from the fixed disk, the driving piece is configured to perform periodic actions, and each action cycle comprises: the movable disc which keeps a set distance from the fixed disc is driven to move away from the fixed disc, the valve plates on the movable disc are in a closed position under the pulling of the pulling rope in the moving process, the valve plates on the fixed disc overcome the suction force of the magnetic suction piece and rotate to be opened, and at the moment, condensed water in the condensation pipe is sucked between the movable disc and the fixed disc; then the movable disc is driven to move towards the fixed disc, the valve plate on the fixed disc is in a closed position under the action of pressure and suction of the magnetic suction piece in the moving process, the valve plate on the movable disc rotates and is opened under the action of pressure, and at the moment, condensed water between the movable disc and the fixed disc is pressed into the water bath until the movable disc is contacted with the fixed disc; and then, driving the movable disc to move away from the fixed disc for a set distance, wherein the set distance meets the condition that the valve plate on the fixed disc is still adsorbed by the magnetic piece to be in a closed position and a vacuum space is formed between the movable disc and the fixed disc.
The further improvement lies in that when the movable disc is contacted with the fixed disc, the valve plates of the movable disc are attached to the valve plates of the fixed disc, so that no gap is left between the movable disc and the fixed disc.
The further improvement lies in that the driving piece is a telescopic cylinder, and the output end of the telescopic cylinder penetrates through the end part of the cylinder body and the fixed disc in a movable and sealed mode in sequence and then is connected with the movable disc.
The further improvement is that the time interval of the periodic action of the driving part is 3-10min.
The improved water bath kettle is characterized in that a vertical sliding rail is arranged on the inner wall of the water bath kettle, a lifting frame is connected to the vertical sliding rail in a sliding mode, the inner kettle is installed at the center of the lifting frame, a plurality of counterweight barrels are arranged on the lifting frame and used for loading counterweight substances into the lifting frame to enable the inner kettle to be just completely immersed in water after the articles are placed in the inner kettle and keep a water surface floating state.
The invention has the beneficial effects that:
(1) The device for the biological experiment adopts a closed structure during water bath heating, thereby being beneficial to preventing and controlling the volatile matters of the objects and leading the self protective performance to reach the use requirement of a high-grade biological safety laboratory;
(2) The device for the biological experiment can accurately control the water bath heating temperature by utilizing the characteristic of constant water boiling temperature under a specific pressure during water bath heating, and the heating range can be expanded to be more than 100 ℃; meanwhile, the control of the electric heating element is simplified, and the heating temperature cannot rise due to excessive heat supply; the properties of the heating temperature accuracy, controllability, heatable temperature range and the like of the biological safety laboratory reach the use requirements of a high-grade biological safety laboratory;
(3) The gas generated in the water bath heating process of the device for the biological experiment can be directly emptied after being purified and can also be refluxed again through condensation, so that water is not required to be added periodically, water resources are saved, and the device is particularly suitable for long-time water bath heating experiments; meanwhile, a specific backflow mechanism is adopted, so that boiling water in the water bath can not flow backwards, and condensation of condensed water cannot be influenced by high temperature in the water bath;
(4) This device for it is biological experiments adopts pot structure in floating when water-bath heating, can be along with surface of water automatic rising, and the abundant contact of pot and water improves the stability of heating in can guaranteeing like this all the time.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the purge mechanism;
FIG. 3 is a schematic structural view of a reflow mechanism;
FIG. 4 is an enlarged view of portion A of FIG. 3;
in the figure: 1. a water bath kettle; 2. an electric heating element; 3. an inner pot; 4. a sealing cover; 5. a pressure sensor; 6. an air extracting member; 7. a controller; 8. a purification mechanism; 801. a filter chamber; 802. a disinfection chamber; 803. an ULPA filter; 804. a UV disinfection light source; 805. MPPS leakage detecting port; 9. a condenser tube; 10. a reflux mechanism; 1001. a cylinder body; 1002. fixing the disc; 1003. a movable tray; 1004. a valve plate; 1005. a magnetic member; 1006. a spring; 1007. pulling a rope; 1008. a drive member; 11. a three-way valve; 12. a vertical slide rail; 13. a lifting frame; 14. a counterweight cylinder.
Detailed Description
The present application will now be described in further detail with reference to the drawings, and it should be noted that the following detailed description is given for purposes of illustration only and should not be construed as limiting the scope of the present application, as these numerous insubstantial modifications and variations can be made by those skilled in the art based on the teachings of the present application.
Referring to fig. 1 to 4, a device for biological experiments in a high-level biosafety laboratory is used for heating in a water bath, and comprises a water bath 1 for containing water, wherein an electric heating element 2 for heating and an inner pot 3 for placing articles are arranged in the water bath 1, the electric heating element 2 provides heat to heat the water in the water bath 1, and then the heat is transferred to the articles in the inner pot 3 through the water. In the invention, a water bath kettle 1 is of a sealed structure, a sealing cover 4 which can be opened and closed is arranged at the top of the water bath kettle 1 and is used for putting water and articles, the device also comprises a pressure sensor 5, an air exhaust part 6 and a controller 7, the pressure sensor 5 is arranged in the water bath kettle 1 and is not contacted with water, the input end of the air exhaust part 6 is communicated with the interior of the water bath kettle 1 through a pipeline, and the controller 7 is configured as follows: when the electric heating element 2 is heated, the boiling pressure value corresponding to the boiling of the water just under the preset heating temperature is matched according to the preset heating temperature, the actual pressure value in the water bath 1 is obtained through the pressure sensor 5, the air pumping element 6 is controlled to pump air, the actual pressure value in the water bath 1 is always equal to the boiling pressure value, and the water is boiled under the preset heating temperature.
Through the structural design, on one hand, the invention is beneficial to the prevention and control of volatile matters in the articles, for example, the gas after being pumped out can be treated in a harmless way; on the other hand, the water bath heating temperature can be accurately controlled by utilizing the characteristic that the boiling temperature of water is constant under a specific pressure, and the heating range can be expanded to be more than 100 ℃; at the same time, the control of the electric heating element 2 is simplified, and the heating temperature can not be increased by supplying excessive heat.
For example, in an experiment, 60 ℃ water bath heating is needed, the water is heated to 60 ℃ by the existing water bath heating device through controlling a heating piece, however, the accuracy of control cannot be guaranteed, the water temperature is high or low, the water temperature adjusting process is slow, especially under the condition that the water temperature is high, the water is naturally cooled, and the time is long. The water bath heating device can associate the preset heating temperature with the boiling pressure value through the preset parameter setting of the controller 7, when the water bath heating at 60 ℃ is needed, the controller 7 matches the corresponding boiling pressure value of 20.0kPa, at the moment, in the heating process, the gas generated by boiling is continuously extracted through the air extracting piece 6, the extraction rate is controlled, when the pressure is greater than 20.0kPa, the extraction rate is accelerated, when the pressure is less than 20.0kPa, the extraction rate is reduced, so that the actual pressure value in the water bath kettle 1 is stabilized at 20.0kPa, the heated boiling temperature of the water is stabilized at 60 ℃, the excessive heat energy is supplied, the water temperature cannot rise, and the stability is good. For example, in the experiment, water bath heating (steam sterilization) at 121 ℃ is required, the existing water bath heating device cannot be realized, but the water bath heating device of the invention can be adopted by controlling the actual pressure value to be 205 kPa.
In the present invention, by controlling the actual pressure value, theoretically all heating temperatures below 100 ℃ and above 100 ℃ can be achieved, but considering the performance requirements for the existing air pumping member 6 (e.g. air pressure pump), the currently preferred preset heating temperature is 40-134 ℃, and the corresponding boiling pressure value is 7.4-304.0kPa.
In the invention, the output end of the air extracting piece 6 is connected with a purifying mechanism 8, a filter cavity 801 and a disinfection cavity 802 are formed between the input end and the output end of the purifying mechanism 8, an ULPA filter 803 is arranged in the filter cavity 801, a UV disinfection light source 804 is arranged in the disinfection cavity 802, and an MPPS leakage detecting opening 805 is arranged at the output end position of the purifying mechanism 8. This purification mechanism 8 has realized the purification to steam through high-efficient filtration and UV disinfection, has avoided the pollution to the laboratory environment.
In the invention, in order to be suitable for a long-time water bath heating experiment, the output end of the purification mechanism 8 is connected with the condenser pipe 9 through a pipeline, the condenser pipe 9 is bent in an S shape and extends downwards, the bottom end of the condenser pipe 9 is communicated with the inside of the water bath kettle 1 through the reflux mechanism 10, steam is condensed and then flows back into the water bath kettle 1 again, the height of the water level is kept unchanged, so that water is not required to be added periodically, and the water bath heating experiment is beneficial to saving water resources.
Preferably, a three-way valve 11 is arranged on a connecting pipeline between the purification mechanism 8 and the condensation pipe 9, one interface of the three-way valve 11 is emptied, and the three-way valve 11 is controlled to enable the purification mechanism 8 to be communicated with the condensation pipe 9 or be communicated with the outside air. In the invention, the purified steam can be directly discharged, so that whether a reflux program is started or not can be selected according to requirements, for example, a short-time water bath heating process needs less water, reflux is not needed, water is naturally evaporated and dispersed, and a long-time water bath heating process can lead the steam to enter a condensation reflux program through the regulation of the three-way valve 11.
The backflow mechanism 10 comprises a cylinder 1001, wherein the input end of the cylinder 1001 is communicated with a condensation pipe 9, the output end of the cylinder is communicated with a water bath 1, a fixed disc 1002 and a movable disc 1003 are sequentially intercepted from the input end to the output end in the cylinder 1001, the fixed disc 1002 and the movable disc 1003 are both provided with a rotatable opening and closing valve sheet 1004, a magnetic attraction piece 1005 is arranged on one side, away from the movable disc 1003, of the valve sheet 1004 of the fixed disc 1002 and used for attracting the valve sheet 1004 to be in a closed position, a spring 1006 and a pull rope 1007 are arranged on one side, away from the fixed disc 1002, of the valve sheet 1004 of the movable disc 1003, the spring 1006 is used for pushing the valve sheet 1004 to rotate towards the closed position, and the pull rope 1007 is used for pulling the valve sheet 1004 to be in the closed position;
the active disk 1003 is connected with a driving member 1008 for driving the active disk 1003 to move towards or away from the fixed disk 1002, the driving member 1008 is configured to perform periodic actions, and each action cycle comprises: the movable disc 1003 keeping a set distance from the fixed disc 1002 is driven to move away from the fixed disc 1002, the valve sheet 1004 on the movable disc 1003 is pulled down by a pull rope 1007 to be in a closed position in the moving process, the valve sheet 1004 on the fixed disc 1002 overcomes the suction force of the magnetic suction piece 1005 and rotates to be opened, and at the moment, condensed water in the condensation pipe 9 is sucked between the movable disc 1003 and the fixed disc 1002; then, the movable disc 1003 is driven to move towards the fixed disc 1002, the valve plate 1004 on the fixed disc 1002 is located at a closed position under the action of pressure and suction of the magnetic suction piece 1005 in the moving process, the valve plate 1004 on the movable disc 1003 rotates and opens under the action of pressure, and at the moment, condensed water between the movable disc 1003 and the fixed disc 1002 is pressed into the water bath kettle 1 until the movable disc 1003 contacts with the fixed disc 1002, so that backflow of the condensed water is realized; then, the movable disk 1003 is driven to move away from the fixed disk 1002 for a set distance (the set distance is determined by the size of the suction force of the magnetic element 1005. In the present invention, the magnetic element 1005 with a larger suction force is adopted, and the set distance is controlled to be in a state that the valve sheet 1004 is about to be separated from the magnetic element 1005, so as to improve the vacuum degree), and the set distance satisfies that the valve sheet 1004 on the fixed disk 1002 is still adsorbed by the magnetic element 1005 to be in the closed position, and a vacuum space is formed between the movable disk 1003 and the fixed disk 1002.
It can be seen that in the process of sucking the condensed water between the fixed tray 1002 and the movable tray 1003 and pressing the condensed water into the water bath 1, the condensed water is kept in a blocking state with the inside of the water bath 1, so that the backflow phenomenon cannot occur. In addition, the vacuum space formed between the movable tray 1003 and the fixed tray 1002 can provide a good heat insulation effect, so that the influence of the relatively high temperature in the water bath 1 on the condensed water in the condensation pipe 9 is avoided, and the condensed water is re-evaporated and even boiled.
Preferably, when the movable disc 1003 contacts the fixed disc 1002, the valve plate 1004 of the movable disc 1003 is attached to the valve plate 1004 of the fixed disc 1002, so that no gap is left between the movable disc 1003 and the fixed disc 1002, the vacuum degree of the vacuum space can be ensured as much as possible, and the heat insulation effect is improved.
Preferably, the driving member 1008 is a telescopic cylinder, and an output end of the telescopic cylinder penetrates through the end of the cylinder 1001 and the fixed disc 1002 in a movable and sealed manner in sequence and then is connected with the movable disc 1003. Of course, the driving member 1008 can be of other structures, such as an electric telescopic rod.
Preferably, the time interval for the periodic action of the drive member 1008 is determined by the boiling rate, and the shorter the time interval, typically 3-10min, the faster the boiling, i.e. the action is performed every 3-10min, each action forcing a certain amount of condensed water into the water bath 1. And within 3-10min of the interval, a vacuum space is kept between the movable disc 1003 and the fixed disc 1002 for effective heat insulation.
According to the invention, the inner wall of the water bath kettle 1 is provided with a vertical slide rail 12, the vertical slide rail 12 is connected with a lifting frame 13 in a sliding manner, the inner kettle 3 is arranged at the central position of the lifting frame 13, and the lifting frame 13 is provided with a plurality of counterweight cylinders 14 for loading counterweight substances (such as sand grains) so that the kettle body of the inner kettle 3 is just completely immersed in water after placing articles and keeps a water surface floating state. This floating structure can be along with surface of water automatic rising, can guarantee the abundant contact of interior pot 3 with water like this all the time, improves the stability of heating, is particularly useful for the experiment that the water level reduces along with the steam evacuation gradually.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that various changes and modifications can be made by those skilled in the art without departing from the spirit of the invention, and these changes and modifications are all within the scope of the invention.

Claims (7)

1. The utility model provides a device for biological experiments for high-grade biosafety laboratory, the device is including water bath (1) that is used for holding water, be equipped with in water bath (1) and be used for the electric heat piece (2) of heating and be used for placing object article interior pot (3), a serial communication port, water bath (1) is sealed structure, and the top of water bath (1) is equipped with sealed lid (4) that can open and shut, and the device still includes pressure sensor (5), air exhaust piece (6), controller (7), inside water bath (1) was located in pressure sensor (5), the input of air exhaust piece (6) passes through pipeline and the inside intercommunication of water bath (1), controller (7) are configured into: when the electric heating part (2) is heated, matching a corresponding boiling pressure value when the outlet water just boils at a preset heating temperature according to a preset heating temperature, acquiring an actual pressure value in the water bath (1) through the pressure sensor (5), and controlling the air pumping part (6) to pump air so that the actual pressure value in the water bath (1) is always equal to the boiling pressure value, thereby enabling the water to boil at the preset heating temperature;
the output end of the air extracting piece (6) is connected with a purifying mechanism (8), a filter cavity (801) and a disinfection cavity (802) are formed between the input end and the output end of the purifying mechanism (8), an ULPA filter (803) is arranged in the filter cavity (801), a UV disinfection light source (804) is arranged in the disinfection cavity (802), and an MPPS leakage detecting opening (805) is formed in the output end of the purifying mechanism (8);
the output end of the purification mechanism (8) is connected with a condensation pipe (9) through a pipeline, the condensation pipe (9) bends in an S shape and extends downwards, and the bottom end of the condensation pipe (9) is communicated with the inside of the water bath (1) through a backflow mechanism (10);
the backflow mechanism (10) comprises a cylinder body (1001), the input end of the cylinder body (1001) is communicated with a condensation pipe (9), the output end of the cylinder body is communicated with a water bath (1), a fixed disc (1002) and a movable disc (1003) are sequentially intercepted from the input end to the output end in the cylinder body (1001), rotatable opening and closing valve plates (1004) are arranged on the fixed disc (1002) and the movable disc (1003), a magnetic absorption piece (1005) is arranged on one side, away from the movable disc (1003), of each valve plate (1004) of the fixed disc (1002) and used for absorbing the valve plates (1004) to be in a closed position, a spring (1006) and a pull rope (1007) are arranged on one side, away from the fixed disc (1002), of each valve plate (1004) of the movable disc (1003), the spring (1006) is used for pushing the valve plates (1004) to rotate towards the closed position, and the pull rope (1007) is used for pulling the valve plates (1004) to be in the closed position;
the movable disc (1003) is connected with a driving piece (1008) used for driving the movable disc (1003) to move towards or away from the fixed disc (1002), the driving piece (1008) is configured to perform periodic actions, and each action period comprises: the movable disc (1003) keeping a set distance from the fixed disc (1002) is driven to move away from the fixed disc (1002), a valve plate (1004) on the movable disc (1003) is pulled down by a pull rope (1007) to be in a closed position in the moving process, the valve plate (1004) on the fixed disc (1002) overcomes the suction force of a magnetic suction piece (1005) and is opened in a rotating mode, and at the moment, condensed water in a condensation pipe (9) is sucked between the movable disc (1003) and the fixed disc (1002); then the movable disc (1003) is driven to move towards the fixed disc (1002), the valve plates (1004) on the fixed disc (1002) are located at a closed position under the action of pressure and suction of the magnetic suction piece (1005) in the moving process, the valve plates (1004) on the movable disc (1003) rotate and open under the action of pressure, and at the moment, condensed water between the movable disc (1003) and the fixed disc (1002) is pressed into the water bath kettle (1) until the movable disc (1003) is in contact with the fixed disc (1002); and then, driving the movable disc (1003) to move away from the fixed disc (1002) for a set distance, wherein the set distance meets the condition that the valve sheet (1004) on the fixed disc (1002) is still adsorbed by the magnetic adsorption piece (1005) to be in a closed position and a vacuum space is formed between the movable disc (1003) and the fixed disc (1002).
2. The apparatus for biological experiments in high-level biosafety laboratories according to claim 1, wherein said preset heating temperature is between 40 ℃ and 134 ℃ and the corresponding boiling pressure value is between 7.4 kPa and 304.0kPa.
3. The device for the biological experiment used in the high-level biosafety laboratory according to claim 1, wherein a three-way valve (11) is disposed on a connection pipeline between the purification mechanism (8) and the condensation pipe (9), one port of the three-way valve (11) is evacuated, and the three-way valve (11) is controlled to enable the purification mechanism (8) to communicate with the condensation pipe (9) or communicate with the outside air.
4. The apparatus for the biological experiment used in the high-grade biosafety laboratory according to claim 1, wherein when the movable plate (1003) is in contact with the fixed plate (1002), the valve plate (1004) of the movable plate (1003) is attached to the valve plate (1004) of the fixed plate (1002), so that no gap is left between the movable plate (1003) and the fixed plate (1002).
5. The device for the biological experiment used in the high-grade biosafety laboratory according to claim 1, wherein the driving member (1008) is a telescopic cylinder, and the output end of the telescopic cylinder penetrates through the end of the cylinder body (1001) and the fixed disc (1002) in a movable and sealed mode in sequence and then is connected with the movable disc (1003).
6. The device for biological experiments in a high-grade biosafety laboratory according to claim 1, wherein the time interval for the driving member (1008) to perform the periodic action is 3-10min.
7. The device for the biological experiment used for the high-grade biosafety laboratory according to claim 1, characterized in that the inner wall of the water bath (1) is provided with a vertical slide rail (12), the vertical slide rail (12) is connected with a lifting frame (13) in a sliding way, the inner pot (3) is installed at the central position of the lifting frame (13), and the lifting frame (13) is provided with a plurality of counterweight barrels (14) for loading counterweight substances to enable the inner pot (3) to be just completely immersed in water after placing articles and keep the water surface floating state.
CN202211323362.5A 2022-10-27 2022-10-27 Device for biological experiment for high-grade biological safety laboratory Active CN115624996B (en)

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Citations (6)

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