CN115581657A

CN115581657A - Bifidobacterium longum composition and preparation method and application thereof

Info

Publication number: CN115581657A
Application number: CN202211341590.5A
Authority: CN
Inventors: 蔡建平; 刘�英
Original assignee: Shanghai Jiaxin Biotechnology Co ltd
Current assignee: Shanghai Jiaxin Biotechnology Co ltd
Priority date: 2022-10-29
Filing date: 2022-10-29
Publication date: 2023-01-10

Abstract

The application relates to the technical field of cosmetics, in particular to a bifidobacterium longum composition and a preparation method and application thereof. Bifidobacterium longum composition comprising a carrier and Bifidobacterium longum, said composition having a bacterial content of 6 x 10 ¹⁰ ‑10 ¹² CFU/g; the carrier consists of the following components in parts by weight: whey protein 20-32 parts, 10-25 parts of trehalose, 5-8 parts of chitosan, 5-8 parts of sodium alginate and 0-0.3 part of nicotinamide. The carrier and the bifidobacterium longum are compounded for use, so that the bifidobacterium longum can be used as an essence raw material to be applied to the field of cosmetics, and the long-acting and lasting moisturizing effect on skin can be achieved while the blank of the field of cosmetics is filled.

Description

Bifidobacterium longum composition and preparation method and application thereof

Technical Field

The application relates to the technical field of cosmetics, in particular to a bifidobacterium longum composition and a preparation method and application thereof.

Background

Bifidobacterium longum (b.longum) belongs to the genus bifidobacterium of the family of actinomycetes, is a typical probiotic, and has been confirmed by research to have various physiological health-care functions on human bodies, such as improving the intestines and stomach of human bodies, nourishing, resisting aging, resisting tumors, improving immunity and the like, but the study on bifidobacterium in China is still in the research and development stage at present, the product development direction is relatively dangerous, and the bifidobacterium longum is generally only produced and processed into a probiotic medicament for conditioning the intestines, but is relatively rare in the application of cosmetics.

The related art discloses the application of bifidobacterium longum in treating sensitive skin, and provides a cream of bifidobacterium longum extract, wherein the main component of the cream is a lysate of bifidobacterium species, and the cream has special efficacy in treating and/or preventing sensitive skin from being affected by epidermal dry skin diseases.

Although the cream containing the bifidobacterium longum extract overcomes the prejudice of the bifidobacterium longum for internal use, the cream does not fill the blank of the application of the bifidobacterium longum in the field of cosmetics, so that the inventor further develops an essence capable of repairing damaged skin on the basis of the prejudice, and unexpectedly discovers the special effect of the bifidobacterium longum composition on the aspect of moisturizing the skin in the actual research process.

Disclosure of Invention

The application provides the bifidobacterium longum composition serving as the raw material of the essence on the basis of filling up the blank of bifidobacterium longum in the field of cosmetics, and the obtained essence can play long-acting and long-lasting moisturizing and skin moistening effects on skin after being coated on the skin.

In a first aspect, the present application provides a bifidobacterium longum composition, which adopts the following technical scheme:

a Bifidobacterium longum composition comprises carrier and Bifidobacterium longum, and has bacteria content of 6 × 10 ¹⁰ -10 ¹² CFU/g；

The carrier consists of the following components in parts by weight: 20-32 parts of lactalbumin, 10-25 parts of trehalose, 5-8 parts of chitosan, 5-8 parts of sodium alginate and 0-0.3 part of nicotinamide.

By adopting the technical scheme, the bifidobacterium longum composition consisting of the bifidobacterium longum with the thallus concentration and the carrier forms an outer wall by compounding modified and solidified whey protein with trehalose and chitosan, forms a stable microemulsion gel structure by taking the bifidobacterium longum, sodium alginate and nicotinamide as inner cores, has long-acting and durable moisturizing effect when being applied to essence, has the moisture content of 39.32-43.52 in the eighth day of use, and has the moisture content increase rate of 23.2-34.9%.

The reason for analyzing the micro-emulsion gel structure is probably that the micro-emulsion gel structure effectively reduces the loss of strains and effective substances in the inner core through the protection of the outer wall on the inner core and the oxygen isolation effect, the basic effective activity of the bacteria is higher, when the micro-emulsion gel structure is rubbed and smeared on the skin and the outer wall is broken, the effective components in the inner core are released to the surface layer of the skin, and can quickly permeate into skin tissues, and simultaneously, the free radicals and epidermic bacteria microorganisms can be effectively reduced, so that the activity and the hydration degree of the epidermis are obviously improved, and in addition, a lubricating protective film can be formed on the surface of the skin, and the water loss of the epidermis can be effectively prevented.

Preferably, the carrier consists of the following components in parts by weight: 20-26 parts of lactalbumin, 15-20 parts of trehalose, 5-6 parts of chitosan, 5-6 parts of sodium alginate and 0.1-0.3 part of nicotinamide.

By adopting the technical scheme, the carrier with the optimized components has a better compounding effect with the bifidobacterium longum, and the sodium alginate and the nicotinamide in the carrier can be combined with the bifidobacterium longum to obviously inhibit the generation of free radicals, so that the vitality and the hydration degree of the skin are ensured.

Preferably, the bifidobacterium longum is one or more of BBMN68, BB536 and BL 21.

By adopting the technical scheme, the bifidobacterium longum strain is 6 x 10 ¹⁰ -10 ¹² CFU/g has high effective activity, and can rapidly permeate into skin tissue, and simultaneously can be compounded with other components to reduce the generation of free radicals and epidermic bacteria microorganisms, thereby improving the activity and the hydration degree of the epidermis.

In a second aspect, the present application provides a method for preparing a bifidobacterium longum composition, which adopts the following technical scheme: a method for preparing a Bifidobacterium longum composition, comprising the steps of:

a1, mixing and stirring whey protein, trehalose, chitosan and sodium alginate, adjusting the pH value to 5.0-7.0, and heating at 60-65 ℃ to deform and solidify the mixture to obtain a gelatinous mixture;

and A2, cooling the gel mixture to 30-50 ℃, adding bifidobacterium longum and nicotinamide, mixing, filtering and washing in a sterile manner, and freeze-drying to obtain the bifidobacterium longum composition.

By adopting the technical scheme, the preparation steps are simple, various conditions are easy to control, and meanwhile, the obtained bifidobacterium longum composition has high effective activity of strains, stable and uniform performance, can effectively form a microemulsion gel structure, and improves the activity and the hydration degree of epidermis.

Preferably, the Bifidobacterium longum is further subjected to ultrasonic wall breaking treatment, i.e. treatment at a power of 80-120W for 10-30S, before being added into the gel-like mixture.

By adopting the technical scheme, the broken product of the bifidobacterium longum subjected to the ultrasonic wall-breaking treatment contains a large amount of effective components capable of promoting the activity and the hydration degree of the epidermis, is easier to be absorbed by the epidermis, and can fully permeate into skin tissues to play a role in maintaining and moisturizing the skin.

In a third aspect, the present application provides an application of a bifidobacterium longum composition, which adopts the following technical scheme:

the application of the bifidobacterium longum composition in preparing the essence can achieve long-acting and lasting moisturizing and skin moistening effects on skin.

Preferably, the essence comprises the following components in parts by weight: 2-16 parts of bifidobacterium longum composition, 0.2-0.5 part of pH regulator, 0.01-0.03 part of chelating agent, 0.1-0.3 part of antioxidant, 0.3-0.6 part of emulsifier and 50-80 parts of deionized water.

By adopting the technical scheme, the essence with the components can quickly permeate into skin tissues, remarkably reduce the generation of free radicals and epidermic bacteria microorganisms through the multi-component compounding effect, and form a lubricating protective film on the surface of the skin, thereby playing a long-acting and lasting role in maintaining and moistening the skin.

Preferably, the essence also comprises 4-8 parts of humectant and 0.8-2.4 parts of emollient;

the humectant is one or more of butanediol, stearic acid monoglyceride, palmitic acid and beta-glucan;

the emollient is one or more of cyclopentadimethylsiloxane, glycerol tri (ethyl hexanoate), lecithin and lanolin.

By adopting the technical scheme, the humectant and the emollient are mixed, so that the moisturizing effect of the bifidobacterium longum composition can be further enhanced, and the formation of a lubricating and protecting film on the surface of skin can be promoted, so that the long-acting and durable skin caring effect can be achieved.

In summary, the present application has the following beneficial effects:

1. by compounding the carrier and the bifidobacterium longum, the bifidobacterium longum can be used as an essence raw material to be applied to the field of cosmetics, so that the blank of the field of cosmetics is filled, and meanwhile, the long-acting and long-lasting moisturizing effect on skin can be achieved;

2. the bifidobacterium longum composition in the application is a microemulsion gel structure formed by compounding modified and solidified whey protein with trehalose and chitosan together to form an outer wall and taking bifidobacterium longum, sodium alginate and nicotinamide as an inner core, and the basic effective activity of thalli in the microemulsion gel structure is higher and the application effect is better;

3. the preparation steps are simple and easy to control, all conditions are easy to control, the obtained bifidobacterium longum composition is stable and uniform in performance, can effectively form a microemulsion gel structure, is high in effective activity of strains, and can effectively improve the activity and the hydration degree of epidermis;

4. after the bifidobacterium longum composition is prepared into essence, the generation of free radicals and epidermal bacteria microorganisms can be remarkably reduced through the multi-component compounding effect, and a lubricating protective film is formed on the surface of skin, so that the long-acting and durable skin-nourishing effect is achieved.

Detailed Description

The present application is further illustrated in detail with reference to the following examples:

the materials and equipment used in the examples and assays of this application are commercially available except as specifically described below: the frankincense used in the application is Elephantia carterii, which is taken as a research object, wherein the main component of volatile oil is blank value (MMV) and the content of total triterpenoid organic acid is 5.6 percent by gas chromatography, the content of total organic acid is 47.8 percent by ultraviolet spectrophotometer, and the content of skin moisture content increase rate is 26.5 percent by ultraviolet spectrophotometer;

polymer (b): polyglycerol surfactants such as: polyglycerol-5 trioleate and polyglycerol-10 diisostearate, purchased from sun chemical, japan;

detection apparatus UV-2201 type ultraviolet-visible spectrophotometer, purchased from Shimadzu corporation, japan;

the detection device Agilent 6890N FID detector gas chromatography analyzer is purchased from Agilent, inc. in America.

Preparation example

Preparation examples 1 to 6

A Bifidobacterium longum composition comprises vehicle and Bifidobacterium longum, and has bacteria content of 6 × 10 ¹⁰ CFU/g, wherein each component of the carrier and the corresponding weight parts are shown in table 1, and the bifidobacterium longum is BBMN68;

the preparation method comprises the following steps:

a1, firstly mixing and stirring whey protein, trehalose, chitosan and sodium alginate at the speed of 800r/min for 30min, then adding citric acid to adjust the pH value to 5.0, and then heating at 60 ℃ to deform and solidify the mixture to obtain a gel mixture;

and A2, cooling the gel mixture to 30 ℃, adding the bifidobacterium longum and the nicotinamide, mixing for 15min at the speed of 1000r/min, carrying out sterile filtration, washing with deionized water, and carrying out freeze drying at the temperature of 0 ℃ for 20min to obtain the bifidobacterium longum composition.

TABLE 1 Components and weights (kg) of carriers in preparation examples 1-6

Preparation example 7

Bifidobacterium longum compositionThe bacteria content of the composition is 6 x 10 ¹¹ The procedure of preparation example 1 was repeated except for CFU/g.

Preparation example 8

A Bifidobacterium longum composition contains bacteria 6 × 10 ¹² The procedure of preparation example 1 was repeated except for CFU/g.

Comparative preparation example 1

A Bifidobacterium longum composition contains bacteria 2 × 10 ¹⁰ The procedure of preparation example 1 was repeated except for CFU/g.

Comparative preparation example 2

A Bifidobacterium longum composition contains bacteria 8 × 10 ¹² The procedure of preparation example 1 was repeated except for CFU/g.

Preparation example 9

A Bifidobacterium longum composition was prepared in the same manner as in production example 1 except that the Bifidobacterium longum in the composition was BB 536.

Preparation example 10

A Bifidobacterium longum composition was prepared in the same manner as in production example 1 except that the Bifidobacterium longum of the composition was BL 21.

Preparation example 12

A Bifidobacterium longum composition, which is different from preparation example 1 in that it is prepared by the following preparation method:

a1, firstly mixing and stirring whey protein, trehalose, chitosan and sodium alginate at the speed of 800r/min for 30min, then adding citric acid to adjust the pH value to 6.0, and then heating at 62 ℃ to deform and solidify the mixture to obtain a gel mixture;

and A2, cooling the gel mixture to 40 ℃, adding the bifidobacterium longum and the nicotinamide, mixing for 15min at the speed of 1000r/min, carrying out sterile filtration, washing with deionized water, and carrying out freeze drying at the temperature of 0 ℃ for 20min to obtain the bifidobacterium longum composition.

Preparation example 13

a1, firstly mixing and stirring whey protein, trehalose, chitosan and sodium alginate at the speed of 800r/min for 30min, then adding citric acid to adjust the pH value to 7.0, and then heating at 65 ℃ to deform and solidify the mixture to obtain a gel mixture;

and A2, cooling the gel mixture to 50 ℃, adding the bifidobacterium longum and the nicotinamide, mixing for 15min at the speed of 1000r/min, carrying out sterile filtration, washing with deionized water, and carrying out freeze drying at the temperature of 0 ℃ for 20min to obtain the bifidobacterium longum composition.

Preparation example 14

a1, mixing and stirring whey protein, trehalose, chitosan and sodium alginate at a speed of 800r/min for 30min, adding citric acid to adjust the pH value to 5.0, and heating at 60 ℃ to deform and solidify the mixture to obtain a gelatinous mixture;

then, carrying out ultrasonic wall breaking treatment on the bifidobacterium longum, namely treating for 30S at the power of 80W to obtain a crushed product; and A2, cooling the gel mixture to 30 ℃, adding bifidobacterium longum (crushed product) and nicotinamide, mixing for 15min at the speed of 1000r/min, performing sterile filtration, washing with deionized water, and performing freeze drying at the temperature of 0 ℃ for 20min to obtain the bifidobacterium longum composition.

Preparation example 15

A bifidobacterium longum composition which is different from the preparation example 1 in that the composition is prepared by the following preparation method:

then, carrying out ultrasonic wall breaking treatment on the bifidobacterium longum, namely treating for 20S at the power of 100W to obtain a broken product; and A2, cooling the gel mixture to 30 ℃, adding bifidobacterium longum (crushed product) and nicotinamide, mixing for 15min at the speed of 1000r/min, performing sterile filtration, washing with deionized water, and performing freeze drying at the temperature of 0 ℃ for 20min to obtain the bifidobacterium longum composition.

Preparation example 16

then, carrying out ultrasonic wall breaking treatment on the bifidobacterium longum, namely treating for 10S at the power of 120W to obtain a broken product; and A2, cooling the gel mixture to 30 ℃, adding bifidobacterium longum (crushed product) and nicotinamide, mixing for 15min at the speed of 1000r/min, performing sterile filtration, washing with deionized water, and performing freeze drying at the temperature of 0 ℃ for 20min to obtain the bifidobacterium longum composition.

Detection test

The essences prepared in each example and comparative example are used as detection objects, the change value of moisture and the increase rate of moisture content after the application are tested, the test group is composed of 100 volunteers with healthy skin and 20-45 years old, the test group is randomly divided into 25 groups, each group comprises 4 persons, and the specific test method and the detection steps are as follows:

before the test, the test subject needs to use Corneometer CM825 PC to obtain blank value (MMV) of the test part (i.e. the area to be coated) ₀ ) Measuring, continuously using corresponding essence for 8 days, respectively in the morning and evening, 3-5 drops (2 ml) each time, and testing water content (MMV) at the same time period after 8 days _t ) And the average value of each detection result is recorded in the following table.

Wherein the moisture content increase rate of skin = (MMV) _t -MMV ₀ ) 0 x 100% of MMV, namely using the average value of final detection after 8 days to subtract a blank value to obtain the change value of the MMV in the period, and then removing the blank value to calculate the growth rate of the MMV.

Examples

Examples 1 to 6

An essence comprises the components and corresponding parts by weight shown in Table 2, and is prepared by stirring at 800r/min for 30 min;

wherein the bifidobacterium longum composition is prepared by the preparation example 1; the pH regulator is citric acid; the chelating agent is EDTA; the antioxidant is BHT; the emulsifier is sorbitan monopalmitate.

Table 2 the components and weights (kg) of the essences of examples 1-6

The essences prepared in the above examples 1 to 6 were extracted, the moisture content and the skin moisture content increase rate thereof after eight days of use were measured according to the above measurement method, and the measurement results were averaged and recorded in the following table.

As can be seen from the above table, the blanks (MMV) of the essences obtained in examples 1-6 were only 30.18-33.08; after trial for 8 days, the water content (MMVt) is increased to 39.32-42.78, and the increase rate of the water content is as high as 23.2-31.2%;

the essence with the components can quickly permeate into skin tissues, remarkably reduce the generation of free radicals and epidermic bacteria microorganisms through the compounding effect of multiple components, and form a lubricating protective film on the surface of the skin, so that the long-acting and durable skin care effect is achieved.

It can also be seen from the table that examples 3-4 are preferred examples, and it can be seen that the skin moisturizing effect of the essence with the above components is optimal, and the skin moisturizing effect does not continue to increase as expected with the increase of the amount of the bifidobacterium longum composition, and the reason for analyzing this may be that the excessive amount of the bifidobacterium longum composition increases the respiratory load of the skin surface layer instead because the production of free radicals and epidermal bacterial microorganisms on the skin surface has been suppressed to the minimum threshold, thereby slowing down the absorption of moisture by the skin, see examples 5-6.

Examples 7 to 11

The essence is different from the essence of example 1 in the using condition of the used bifidobacterium longum composition, and the specific corresponding relation is shown in the table below.

Table: EXAMPLES 7-11 use of Bifidobacterium longum compositions

Group of	Bifidobacterium longum composition
		Example 7	Prepared from preparation example 2
Example 8	Prepared from preparation example 3
		Example 9	Prepared from preparation example 4
Example 10	Prepared from preparation example 5
		Example 11	Prepared from preparation example 6

The essences prepared in the above examples 7 to 11 were extracted, and the moisture content and the skin moisture content increase rate thereof after eight days of use were measured according to the above measurement method, and the measurement results were averaged and recorded in the following table.

As can be seen from the above table, the serum obtained in examples 7 to 11 had a blank value (MMV) of only 31.16 to 32.38; after trial for 8 days, the moisture content (MMVt) is increased to 41.58-43.52, and the moisture content increase rate is as high as 33.4-34.9%;

therefore, the bifidobacterium longum composition with the components can endow the essence with long-acting and long-lasting moisturizing and skin moistening effects when being applied to the essence, and the bifidobacterium longum composition exists in the essence in a microemulsion gel structure, so that the activity of effective components of thalli can be guaranteed.

In addition, from examples 7 to 11, it is also known that nicotinamide can significantly inhibit the generation of free radicals by being combined with bifidobacterium longum and sodium alginate, thereby further improving the moisturizing effect of the essence.

As can be seen from the above table, example 9 is a preferred example, and its blank value (MMV) is only 32.22; after trial for 8 days, the moisture content (MMVt) of the product is increased to 43.48, and the moisture content increase rate is as high as 34.9%; the component can be seen as the bifidobacterium longum composition with the optimal proportion.

When the essence needs to be used, the bifidobacterium longum composition with the microemulsion gel structure is broken on the outer wall in the process of rubbing and smearing the composition on skin, the active ingredients in the inner core of the composition are released to the surface layer of the skin and quickly permeate into skin tissues, and free radicals and epidermal bacterial microorganisms are effectively reduced, so that the activity and the hydration degree of the epidermis are obviously improved, and in addition, a lubricating protective film can be formed on the surface of the skin, so that the moisture loss of the epidermis can be effectively prevented, and a long-acting moisturizing effect is achieved.

Examples 12 to 13 and comparative examples 1 to 2

Table: comparative examples 1-2, examples 12-13, and comparative examples 1-2

Group of	Bifidobacterium longum composition
		Example 12	Prepared from preparation example 7
Example 13	Prepared from preparation example 8
		Comparative example 1	From comparative preparation example 1
Comparative example 2	From comparative preparation example 2

The essences prepared in the above examples 12 to 13 and comparative examples 1 to 2 were extracted, the moisture content and the skin moisture content increase rate after eight days of use thereof were measured according to the above measurement methods, and the measurement results were averaged and recorded in the following table.

As can be seen from the above table, the serum obtained in examples 1, 12-13 had a blank value (MMV) of only 30.18-32.28; after trial for 8 days, the water content (MMVt) is increased to 39.32-40.03, and the increase rate of the water content is as high as 23.2-24.1%;

it can be seen that the above-mentioned Bifidobacterium longum bacteria have high effective activity, and can quickly permeate into skin tissue, and at the same time can be compounded with other components so as to reduce production of free radical and epidermic bacteria microorganism, so that it can raise epidermic vitality and hydration degree, and when the bacteria content is too low, its moisture-retaining effect is reduced, and when the bacteria content is continuously increased, its moisture-retaining and skin-moistening effect is not increased as expected, see comparative examples 1-2, so that 8 x 10 is comprehensively considered ⁹ -10 ¹² The bacterial content of CFU/g was set as the optimum value.

Examples 14 to 15

Table: EXAMPLES 14-15 use of Bifidobacterium longum compositions

Group of	Bifidobacterium longum composition
		Example 14	Prepared from preparation example 9
Example 15	Prepared from preparation example 10

The essences prepared in the above examples 14 to 15 were extracted, and the moisture content and the skin moisture content increase rate thereof after eight days of use were measured according to the above measurement method, and the measurement results were averaged and recorded in the following table.

As can be seen from the above table, the blank value (MMV) of the essence obtained in the examples 1 and 14 to 15 is only 30.18 to 31.32; after trial for 8 days, the moisture content (MMVt) is increased to 39.32-38.52, and the moisture content increase rate is as high as 22.8-23.2%;

it can be seen that the above species of Bifidobacterium longum are 8 × 10 ⁹ -10 ¹² Has higher effective activity under the bacteria content of CFU/gBesides rapid penetration into skin tissue, the composition can be compounded with other components to reduce the generation of free radicals and epidermal bacterial microorganisms, thereby improving the activity and hydration of the epidermis.

Examples 16 to 20

Table: EXAMPLES 16-20 use of Bifidobacterium longum compositions

Group of	Bifidobacterium longum composition
		Example 16	Prepared from preparation example 12
Example 17	Prepared from preparation example 13
		Example 18	Prepared from preparation 14
Example 19	Prepared from preparation example 15
		Example 20	Prepared from preparation example 16

The essences prepared in the above examples 16 to 20 were extracted, and the moisture content and the skin moisture content increase rate thereof after eight days of use were measured according to the above measurement method, and the measurement results were averaged and recorded in the following table.

As can be seen from the above table, the blanks (MMV) of the essences obtained in examples 1 and 16-20 are only 30.18-31.22; after trial for 8 days, the moisture content (MMVt) is increased to 37.45-40.06, and the moisture content increase rate is as high as 23.2-28.5%;

the bifidobacterium longum composition obtained by the preparation method has high effective activity of strains, stable and uniform performance, can effectively form a microemulsion gel structure, and improves the activity and the hydration degree of epidermis according to the examples 1 and 16-17.

From examples 18 to 20, it can be seen that the bifidobacterium longum after the ultrasonic wall-breaking treatment contains a large amount of effective components capable of promoting the vitality and the hydration degree of the epidermis in the broken product, is easier to be absorbed by the epidermis, and can fully permeate into skin tissues to achieve the effects of maintaining and moisturizing the skin.

Examples 21 to 25

The essence is different from the essence in example 1 in that the components and the corresponding parts by weight are shown in table 3, and the essence is prepared by stirring at 800r/min for 30min and uniformly mixing;

wherein the humectant is prepared by compounding butanediol and glycerol monostearate according to the weight ratio of 1; the emollient consists of cyclopentadimethylsiloxane, lecithin and lanolin in a weight ratio of 1.

TABLE 3 the components and weights (kg) of the essences of examples 21-25

The essences prepared in the above examples 21 to 25 were extracted, and the moisture content and the skin moisture content increase rate thereof after eight days of use were measured according to the above measurement method, and the measurement results were averaged and recorded in the following table.

As can be seen from the above table, the blank value (MMV) of the essences obtained in examples 1 and 21-25 is only 30.18-32.11; after trial for 8 days, the water content (MMVt) is increased to 39.32-42.77, and the increase rate of the water content is as high as 23.2-33.3%;

therefore, the humectant and the emollient can further enhance the moisturizing effect of the bifidobacterium longum composition and promote the formation of a lubricating and protecting film on the surface of skin by mixing the humectant and the emollient, so that the long-acting and lasting skin-nourishing effect is achieved.

The specific embodiments are only for explaining the present application and are not limiting to the present application, and those skilled in the art can make modifications to the embodiments without inventive contribution as required after reading the present specification, but all the embodiments are protected by patent law within the scope of the claims of the present application.

Claims

1. A Bifidobacterium longum composition comprising a carrier and Bifidobacterium longum, said composition having a bacterial content of 6 x 10 ¹⁰ -10 ¹² CFU/g；

2. The bifidobacterium longum composition of claim 1 wherein the carrier is comprised of the following components in parts by weight: 20-26 parts of lactalbumin, 15-20 parts of trehalose, 5-6 parts of chitosan, 5-6 parts of sodium alginate and 0.1-0.3 part of nicotinamide.

3. A bifidobacterium longum composition as claimed in claim 1 wherein the bifidobacterium longum is one or more of BBMN68, BB536 and BL 21.

4. A method of producing a bifidobacterium longum composition as claimed in any of claims 1 to 3, comprising the steps of:

and A2, cooling the gel mixture to 30-50 ℃, adding the bifidobacterium longum and the beta-nicotinamide, mixing, filtering and washing in a sterile manner, and freeze-drying to obtain the bifidobacterium longum composition.

5. A process for the preparation of a Bifidobacterium longum composition as claimed in claim 4, wherein the Bifidobacterium longum is subjected to an ultrasonic wall-breaking treatment at a power of 80-120W for 10-30S before being added to the gel-like mixture.

6. The use of a Bifidobacterium longum composition as claimed in any of claims 1 to 3 for the preparation of a serum having a long lasting moisturising effect on the skin.

7. The use of the bifidobacterium longum composition as claimed in claim 6, wherein the essence comprises the following components in parts by weight: 2-16 parts of bifidobacterium longum composition, 0.2-0.5 part of pH regulator, 0.01-0.03 part of chelating agent, 0.1-0.3 part of antioxidant, 0.3-0.6 part of emulsifier and 50-80 parts of deionized water.

8. The use of a bifidobacterium longum composition as claimed in claim 7, wherein the essence further comprises 4 to 8 parts of a humectant and 0.8 to 2.4 parts of an emollient;

the humectant is one or more of butanediol, glyceryl monostearate, palmitic acid and beta-glucan;