CN115553184A - Method for promoting germination of submerged plant tape grass seeds and seedling growth - Google Patents
Method for promoting germination of submerged plant tape grass seeds and seedling growth Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
The invention discloses a method for promoting germination of submerged plant tape grass seeds and growth of seedlings, which comprises the following steps: (1) seed collection: selecting bitter grass seed pods, soaking the bitter grass seed pods in pure water, extruding out seeds inside the bitter grass seed pods, and cleaning the bitter grass seed pods with the pure water; (2) sterilization treatment: soaking the seeds in a sodium hypochlorite solution, and cleaning the seeds by pure water; preparing a 6-benzylaminopurine treatment solution: dissolving 6-BA in hydrochloric acid solution, and fixing the volume with pure water; (4) seed pretreatment: completely soaking the sterilized tape grass seeds into the 6-BA treatment solution, placing the tape grass seeds in a thermostatic chamber, and cleaning the tape grass seeds with pure water; (5) sowing: putting the seeds of the tape grass on the surface of the lake sediment; (6) setting culture environment: after sowing, placing the culture container in a constant temperature chamber for germination; (7) seedling growth: continuously culturing in a constant temperature chamber, and keeping the tape grass seedlings immersed. The method has the advantages of low economic cost, wide application range, simple operation and very obvious promotion effect, and effectively promotes the growth of the germinated tape grass seedlings.
Description
Technical Field
The invention belongs to the technical field of restoration of bitter grass vegetation in eutrophic lakes, and particularly relates to a method for promoting germination of seeds and growth of seedlings of submerged plants, namely bitter grass.
Background
The bitter herbs (Vallisneria natana) are submerged plants in the genus of bitter herbs in the family of Amyda sinensis, and are one of the most widely distributed submerged plants in China. At present, the lake ecosystem is gradually degraded, the eutrophication problem is increasingly prominent, and the recovery of submerged plants is an important measure for improving the water quality and the ecological environment. The eel grass has the characteristics of strong vitality, rapid growth, wide application range to the environment and the like, and is widely applied to water purification and water ecological restoration. Besides the asexual clonal growth, the tape grass can also be propagated sexually, and a large number of seeds can be generated in the process of the life history of the tape grass, which has important significance for recovering the tape grass population in a large area. The tape grass population is recovered in the degenerated lake, and besides the tape grass adult plants are planted, seed sources can be supplemented by sowing seeds, so that the tape grass seed-sowing method has the advantage of low cost. However, due to the complexity and variability of environmental factors and the limitation of biological characteristics of seeds, the germination rate and seedling rate of the eel grass seeds in eutrophic lakes are low, and the recovery of eel grass populations by a direct sowing method is difficult.
The rapid germination of the tape grass seeds and the normal growth of the seedlings after germination are important prerequisites for the recovery of the tape grass. At present, the method for promoting the germination of the seeds and the growth of the seedlings of the tape grass has a method for changing a growth matrix, and a Chinese patent 'a method for promoting the germination of the seeds and the growth of the seedlings of the tape grass of submerged plants (application number: CN 201811182864.4') comprises the following steps: 1) Spreading the tape grass seeds on the surface or in holes of the porous carbon material, and after the tape grass seeds germinate and seedlings grow with roots, placing the porous carbon material and the tape grass seedlings in water with good illumination conditions; 2) And adjusting the height of the seedling in the water body, and ensuring that the tape grass seedling can grow quickly. However, the preparation process of the porous carbon material is complex, large-scale preparation is difficult, the investment cost is high, and large-scale popularization and application are difficult in the actual eel grass recovery engineering.
Secondly, a method for pretreating seeds by chemical reagents is provided, and the Chinese patent 'a method for promoting the germination and growth of eel grass seeds (application number: CN 201110002505)' comprises the following steps: 1) Adding potassium nitrate solution or chitosan solution in the incubator, and adding herba Swertiae Dilutae seeds for soaking with trace intermittent aeration; 2) Culturing the soaked bitter grass seeds in a beaker filled with lake sediment as a matrix; 3) After the seeds of the tape grass germinate, the seedlings of the tape grass are soaked in an incubator filled with chitosan solution, and trace intermittent aeration and intermittent short-wave ultraviolet irradiation are assisted. The optimal treatment concentration of the chemical reagent used in the method is difficult to determine, and irreversible damage to the seeds can be caused if the treatment concentration is too high. Meanwhile, the invention also needs to soak the seedlings with chitosan to promote the growth of the seedlings, is difficult to be practically operated in the recovery engineering of the lake tape grass and has higher cost.
Phytohormone pretreatment is a common method for promoting seed germination and seedling growth, and mainly comprises auxins, cytokinins and gibberellins. The prior research shows that the treatment of the picrass seeds with indoleacetic acid (IAA) and Kinetin (KT) can promote germination and improve the dry weight and the plant height of seedlings, but the optimal concentration is more than 100mg/L, and the concentration is higher than that of the optimal concentration 8mg/L of potassium nitrate treatment in Chinese patent (application number: CN 201110002505), namely the cost is higher.
Therefore, the key point of the invention is to select a method which has low economic cost, wide application range and simple operation and promotes the germination of the tape grass and the growth of seedlings.
Disclosure of Invention
In order to overcome the defects of the conventional eel grass seed germination method and the existing technical problems, the invention aims to provide the method for promoting eel grass seed germination and seedling growth, which has the advantages of low economic cost, wide application range, simple operation and very obvious promotion effect, effectively improves the germination rate and the germination rate of eel grass seeds, and effectively promotes the growth of germinated eel grass seedlings.
In order to achieve the purpose, the invention adopts the following technical measures:
the technical conception of the invention is as follows: collecting seeds, sterilizing, preparing 6-benzylaminopurine (6-BA) treatment solution, pretreating the seeds, sowing, setting culture environment and growing seedlings.
A method for promoting germination of seeds and seedling growth of submerged plants, namely tape grass, comprises the following steps:
(1) Seed collection: selecting healthy tape grass seed pods without diseases and insect pests, soaking the tape grass seed pods in pure water for 7-9 hours, extruding out internal seeds, and cleaning the seeds with the pure water (2-4 times);
(2) And (3) sterilization treatment: soaking the seeds in 3-5% sodium hypochlorite solution for 14-16 min, and cleaning with pure water (2-4 times) for use;
(3) Preparing a 6-benzylaminopurine (6-BA) treatment solution: dissolving 2.5-7.5 mg of 6-benzylaminopurine (6-BA) in a small amount of 1mol/L hydrochloric acid solution, and diluting to 500 ml with pure water to obtain 5-15mg/L disposable 6-benzylaminopurine (6-BA) treatment solution;
(4) Seed pretreatment: completely immersing the sterilized bitter herb seeds into the 6-benzylaminopurine (6-BA) treatment solution prepared in the step (3), placing the solution in a thermostatic chamber (24-26 ℃) for 22-26 hours, and then cleaning the solution (2-4 times) by using pure water for later use;
(5) Sowing: placing the pretreated eel grass seeds into a culture container which is filled with lake mud with the thickness of 4-10cm and a certain amount of tap water and is kept stand for 22-26 hours;
(6) Setting a culture environment: after sowing, putting the culture container into a thermostatic chamber (24-26 ℃) for germination;
(7) And (3) seedling growth: culturing in a thermostatic chamber for 50 days, replenishing water every week, and keeping the tape grass seedling completely submerged.
As a further technical improvement, the concentration of the 6-benzylaminopurine (6-BA) treatment solution in the step (3) is preferably 10mg/L.
As a further technical improvement, the temperature of the thermostatic chamber in the steps (4) and (6) is 25 ℃, the illumination intensity is 1200-1800lx, and the light-dark ratio is 12/12 hours.
As a further technical improvement, in the step (4), the 6-benzylaminopurine (6-BA) treatment solution is soaked, and then the cleaned tape grass seeds are soaked in pure water for standby application, and the time is not more than 24 hours.
As a further technical improvement, the culture container in the step (5) is a plastic beaker filled with 4-5cm thick lake mud and 6-7cm deep tap water, and is kept stand for 24 hours before sowing; the total nitrogen content of the lake mud is 0.7-2.5g/kg, the total phosphorus content is 0.5-2.0g/kg, and the organic matter content is 4-9%; the eel grass seeds are separately and uniformly placed on the surface of the lake mud.
In the invention, in the most critical step (4), the sterilized tape grass seeds are completely immersed in the 6-benzylaminopurine (6-BA) treatment solution prepared in the step (3), so that the germination rate and the germination speed of the tape grass seeds can be effectively improved, and the growth of germinated tape grass seedlings is promoted. The method is safe and effective, and does not cause any damage to the seeds; the operation is simple, and the growth of the seedlings can be promoted without secondary treatment; the concentration of the used 6-benzylaminopurine (6-BA) treatment liquid is 15mg/L at most, so that the economic cost is low; is suitable for different eutrophic lakes and can be popularized and applied in actual eel grass restoration projects.
Through the technical measures of the seven steps, the germination rate of the tape grass seeds can be improved by 32.68-63.46 percent and the average germination rate can be improved by 32.64-63.44 percent compared with that of untreated seeds at the fourth day after sowing. Meanwhile, the growth of the eel grass seedlings is remarkably promoted. The biomass of seedlings can be increased by 34.41 percent at most, the content of soluble sugar in leaves can be increased by 40 to 56.60 percent at most, the content of soluble protein can be increased by 50 percent at most, and the activity of root systems can be increased by 32.49 to 55.17 percent.
Compared with the prior art, the invention has the following advantages and technical effects:
1. in the invention, the seed pod absorbs water and becomes soft when being soaked, and the tape grass seeds can be easily extruded after 8 hours. The seed of the tape grass is obtained by soaking the tape grass seed pod, so that the integrity of the seed can be effectively maintained, and the quality of the seed source of the tape grass is guaranteed.
2. According to the method, the surface of the tape grass seeds is sterilized by using a 3-5% sodium hypochlorite solution, so that pathogens are prevented from mildewing in the germination process to reduce the germination rate of the seeds, and meanwhile, the sodium hypochlorite is corrosive to soften seed coats of the tape grass seeds, so that the imbibition capacity of the seeds is improved, and radicles are facilitated to break through the seed coats.
3. In the invention, the tape grass seeds are placed on the surface of lake mud to simulate the substrate condition of the actual environment. The total nitrogen content of the lake mud is 0.7-2.5g/kg, the total phosphorus content is 0.5-2.0g/kg, the organic matter content is 4-9%, and the nutrition state covers light pollution to heavy pollution. The method has wide application range and can be popularized and used in the recovery work of the tape grass in different eutrophic lakes.
4. Compared with the prior art, the method has the advantages of simple operation, wide application range and low economic cost, can promote the germination of the eel grass seeds, can also promote the growth of subsequent seedlings to a certain extent, further effectively reduces the nitrogen and phosphorus content of the bottom sediment of the eutrophic lake, inhibits the release of nitrogen and phosphorus nutrient salts in the bottom sediment, and improves and stabilizes the water quality.
Drawings
FIG. 1 is a schematic diagram showing the comparison of germination rates of seeds of Sophora alopecuroides L in the fourth day after 6-BA treatment (5, 10, 15, 20 mg/L) sowing for a control group for promoting germination of seeds and seedling growth of submerged plants of Sophora alopecuroides L;
the germination rates of the tape grass seeds in the control group and the 5, 10, 15 and 20 mg/L6-BA treatment groups are respectively 34.67 +/-3.06, 46.00 +/-3.06, 51.33 +/-4.16, 56.67 +/-4.16 and 33.33 +/-4.16 percent.
FIG. 2 is a comparison of the germination rate of seeds of eel grass on the fourth day after sowing of 6-BA treatment (5, 10, 15, 20 mg/L) for a control group promoting germination of seeds and seedling growth of submerged plants;
the average germination rates of the eel grass seeds in the control group and the 6-BA treatment groups of 5, 10, 15 and 20mg/L are respectively 8.67 +/-0.76, 11.50 +/-1.00, 12.83 +/-1.04, 14.17 +/-1.04 and 8.33 +/-1.04%/d.
FIG. 3 is a comparative illustration of biomass of seedlings of eel grass after sowing 50 days in a control group for promoting germination of seeds and seedling growth of submerged plants of eel grass and 6-BA treatment (5, 10, 15, 20 mg/L) on the seeds of eel grass;
the biomass of the eel grass seedlings after 50 days of sowing in the control group and the 5, 10, 15 and 20 mg/L6-BA treatment groups is respectively 3.40 +/-0.53 g, 3.33 +/-0.38 g, 4.57 +/-0.70 g, 3.97 +/-0.25 g and 3.10 +/-0.20 g.
FIG. 4 is a schematic diagram showing the comparison of soluble sugar content of leaves of seedlings of tape grass after sowing for 50 days in a control group for promoting germination of seeds and seedling growth of submerged plants of tape grass and 6-BA treatment (5, 10, 15, 20 mg/L) of the tape grass seeds;
the soluble sugar content of the leaves of the sown eel grass seedlings after 50 days of sowing in the control group and the 5, 10, 15 and 20 mg/L6-BA treatment groups is respectively 0.15 +/-0.00, 0.21 +/-0.00, 0.24 +/-0.00, 0.21 +/-0.00 and 0.27 +/-0.01 percent.
FIG. 5 is a schematic diagram showing the comparison of soluble protein content of leaves of seedlings of tape grass after sowing in 6-BA treatment (5, 10, 15, 20 mg/L) for 50 days for a control group for promoting germination of seeds and seedling growth of submerged plants of tape grass;
the soluble protein content of the tape grass seedling leaves after 50 days of sowing in the control group and the 5, 10, 15 and 20 mg/L6-BA treatment groups is respectively 1.26 +/-0.17, 1.29 +/-0.09, 1.89 +/-0.02, 1.79 +/-0.24 and 1.39 +/-0.13 mg/g.
FIG. 6 is a comparison of photosynthetic pigment content of leaves of the seedling of tape grass after sowing for 50 days in a control group for promoting germination of seeds and growth of seedlings of the tape grass of submerged plants and 6-BA treatment (5, 10, 15, 20 mg/L) on the tape grass seeds;
the photosynthetic pigment content of the leaves of the tape grass seedlings after 50 days of sowing in the control group and the 5, 10, 15 and 20 mg/L6-BA treatment groups is respectively 0.84 +/-0.05, 0.69 +/-0.00, 0.82 +/-0.01, 0.78 +/-0.07 and 0.78 +/-0.02 mg/g.
FIG. 7 is a comparison of the root activity of the seedlings of the eel grass after 50 days of sowing the seeds of the eel grass in 6-BA treatment (5, 10, 15, 20 mg/L) of a control group for promoting the germination of the seeds and the growth of the seedlings of the eel grass.
The root system activity of the eel grass seedlings after 50 days of sowing in the control group and the 5, 10, 15 and 20 mg/L6-BA treatment groups is 34.93 +/-3.28, 54.20 +/-4.88, 46.28 +/-1.98, 52.83 +/-3.78 and 34.13 +/-3.73 mg/(g.h) respectively.
Detailed Description
The following examples are provided to further illustrate the present invention in order to better understand the present invention, but the present invention is not limited to the following examples.
Example 1:
a method for promoting germination of seeds and seedling growth of submerged plants, namely tape grass, comprises the following steps:
(1) Seed collection: selecting proper amount of herba Ixeritis Sonchifoliae seed pod, soaking in pure water for 8 or 10 or 12 hr, squeezing out internal seed, and cleaning with pure water for 2 or 3 or 4 times;
(2) And (3) sterilization treatment: soaking the seeds in 3-5% sodium hypochlorite solution for 15 min, and cleaning with pure water for 2 or 3 or 4 times;
(3) Preparing a 6-benzylaminopurine (6-BA) treatment solution: dissolving 2.5 mg of 6-benzylaminopurine (6-BA) in a small amount of 1mol/L hydrochloric acid solution, and diluting to 500 ml with pure water to prepare 5mg/L disposable 6-benzylaminopurine (6-BA) treatment solution; (4) seed pretreatment: completely immersing the sterilized eel grass seeds into the 6-benzylaminopurine (6-BA) treatment solution prepared in the step (3), placing the eel grass seeds in a thermostatic chamber with the temperature of 25 ℃, the illumination intensity of 1200-1800lx and the light-dark ratio of 12/12 hours for 24 hours, and then cleaning the eel grass seeds with pure water for 2 or 3 or 4 times for later use;
(5) Preparing a matrix: selecting a 500 ml plastic beaker as a culture container, paving 4cm thick lake mud in the plastic beaker, adding 6cm deep tap water, and standing for 24 hours; the lake mud is collected from large eutrophic urban lakes-Wuhan east lake. The total nitrogen, total phosphorus and organic matter contents of the lake mud are respectively 1.77 +/-0.01 g/kg, 1.88 +/-0.04 g/kg and 8.13 +/-0.33 percent, which are heavy pollution degrees;
(5) Sowing: the preprocessed tape grass seeds are separately and evenly placed on the surface of the lake mud in a culture container, and 50 tape grass seeds are placed in the culture container;
(6) Setting a culture environment: after sowing, putting the culture container into a thermostatic chamber with the temperature of 25 ℃, the illumination intensity of 1200-1800lx and the light-dark ratio of 12/12 hours for germination;
(7) And (3) seedling growth: culturing in a thermostatic chamber for 50 days, replenishing water every week, and keeping the tape grass seedling completely submerged. After 50 days, all the seedlings of tape grass in the culture vessel were collected and their biomass was measured. Taking a proper amount of leaves, and measuring the content of soluble sugar, soluble protein and chlorophyll a. Taking a proper amount of root system, and measuring the activity of the root system.
Example 1 began at 3 months 2022. Four days after sowing, the germination rate of the tape grass seeds is 46.00 +/-3.06 percent, and the average germination rate is 11.50 +/-1.00 percent/d. At 50 days, the biomass of the tape grass seedling is 3.33 +/-0.38 g, the contents of soluble sugar, protein and photosynthetic pigment of the leaf are respectively 0.21 +/-0.00%, 1.29 +/-0.09 mg/g and 0.69 +/-0.00 mg/g, and the root activity is 54.20 +/-4.88 mg/(g.h).
Example 2:
the difference between the embodiment and the embodiment 1 is that in the step (3), 5mg of 6-benzylaminopurine (6-BA) is dissolved in a small amount of 1mol/L hydrochloric acid solution, and pure water is used for metering to 500 ml to prepare 10mg/L disposable 6-benzylaminopurine (6-BA) treatment solution; the rest is the same as in example 1.
Example 2 began at 3 months 2022. Four days after sowing, the germination rate of the tape grass seeds is 51.33 +/-4.16 percent, and the average germination rate is 12.83 +/-1.04 percent/d. At 50 days, the biomass of the tape grass seedling is 4.57 +/-0.70 g, the contents of soluble sugar, protein and photosynthetic pigment of the leaf blade are respectively 0.24 +/-0.00%, 1.89 +/-0.02 mg/g and 0.82 +/-0.01 mg/g, and the root activity is 46.28 +/-1.98 mg/(g.h).
Example 3:
the difference between the embodiment and the embodiment 1 or 2 is that in the step (3), 7.5 mg of 6-benzylaminopurine (6-BA) is dissolved in a small amount of 1mol/L hydrochloric acid solution, pure water is used for fixing the volume to 500 ml, and a disposable 6-benzylaminopurine (6-BA) treatment solution of 15mg/L is prepared; the rest is the same as in example 1 or 2.
Example 3 began at 3 months 2022. Four days after sowing, the germination rate of the tape grass seeds is 56.67 +/-4.16 percent, and the average germination rate is 14.17 +/-1.04 percent/d. At 50 days, the biomass of the tape grass seedling is 3.97 +/-0.25 g, the contents of soluble sugar, protein and photosynthetic pigment of the leaf blade are respectively 0.21 +/-0.00%, 1.79 +/-0.24 mg/g and 0.78 +/-0.07 mg/g, and the root activity is 52.83 +/-3.78 mg/(g.h).
Example 4:
the difference between the embodiment and the embodiment 1-3 is that in the step (3), 10mg of 6-benzylaminopurine (6-BA) is dissolved in a small amount of 1mol/L hydrochloric acid solution, and pure water is used for metering to 500 ml to prepare a disposable 6-benzylaminopurine (6-BA) treatment solution with 20 mg/L; the rest was the same as in examples 1 to 3.
Example 4 began at 3 months 2022. Four days after sowing, the germination rate of the tape grass seeds is 33.33 +/-4.16 percent, and the average germination rate is 8.33 +/-1.04 percent/d. At 50 days, the biomass of the tape grass seedling is 3.10 +/-0.20 g, the contents of soluble sugar, protein and photosynthetic pigment of the leaf blade are respectively 0.27 +/-0.01%, 1.39 +/-0.13 mg/g and 0.78 +/-0.02 mg/g, and the root activity is 34.13 +/-3.73 mg/(g.h).
Comparative example:
the difference between the comparative example and the examples 1-4 is that the step (3) is omitted, and the step (4) is that the sterilized eel grass seeds are completely immersed into pure water, placed in a thermostatic chamber (24-26 ℃) for 24 hours, and then washed clean by pure water for standby; the rest was the same as in examples 1 to 4.
The control started at 3 months 2022. Four days after sowing, the germination rate of the tape grass seeds is 34.67 +/-3.06 percent, and the average germination rate is 8.67 +/-0.76 percent/d. At 50 days, the biomass of the tape grass seedling is 3.40 plus or minus 0.53g, the contents of soluble sugar, protein and photosynthetic pigment of the leaf are 0.15 plus or minus 0.00%, 1.26 plus or minus 0.17mg/g and 0.84 plus or minus 0.05mg/g respectively, and the root activity is 34.93 plus or minus 3.28 mg/(g.h).
As shown in FIG. 1, the germination rates of the eel grass seeds of examples 1 to 3 were improved by 32.68 to 63.46% after four days of sowing, compared to the control example 1. From the average germination rate (FIG. 2), the average germination rate of the tape grass seeds of examples 1-3 was increased by 32.64-63.44%. Therefore, the germination rate and the germination speed of the tape grass seeds can be effectively improved by carrying out 6-BA treatment on the tape grass seeds.
As shown in fig. 3 to 7, the increase in seedling biomass and protein content was the greatest in example 2, which was 34.41% and 50.00% higher than the control, respectively. The soluble sugar content of examples 1-4 was higher than that of the control, and was highest in example 4, reaching 0.27. + -. 0.01%. However, photosynthetic pigments were reduced in all examples, the least reduced group being example 2 (2.38%).
The root activity of the seedlings in examples 1-3 was increased by 32.49-55.17% compared to the control.
The single index cannot accurately reflect the growth condition of the seedling, so that the membership function method is adopted to carry out standardization and comprehensive evaluation on the seed germination and seedling growth conditions. According to the membership function method, formula 1: membership function value = (measured value of index in a certain group-maximum value of index in all groups)/(maximum value of index in all groups-minimum value of index in all groups) for an index and equation 2: membership function value =1 for an index (measured value for the index in a certain group-maximum value for the index in all groups)/(maximum value for the index in all groups-minimum value for the index in all groups) the indexes related to seed germination and seedling growth in examples 1 to 4 and the control example were calculated and sorted, and the results are shown in table 1.
TABLE 1 membership function values of respective indexes of examples 1 to 4 and comparative examples
The comprehensive value of the membership function can visually reflect the germination and seedling growth conditions of the seeds of the tape grass in the comparison examples and the embodiments, and the larger the comprehensive value is, the better the germination and growth conditions are. As can be seen from Table 1, the comprehensive values of the membership functions of examples 1-4 are all higher than those of the control example, example 2 is the highest, followed by example 3, and then examples 1 and 4, i.e., the germination and seedling growth of the tape grass seeds are found to be 10mg/L >15mg/L >5mg/L >20mg/L > by comprehensive evaluation of germination and seedling growth related indexes. This shows that the 5-15 mg/L6-BA treatment in the invention has very significant promotion effect on the germination of the eel grass seeds and the growth of seedlings, and the 10 mg/L6-BA treatment is the most preferable.
Claims (7)
1. A method for promoting submerged plant tape grass seed germination and seedling growth comprises the following steps:
(1) Seed collection: selecting healthy tape grass seed pods without diseases and insect pests, soaking the tape grass seed pods in pure water for 7-9 hours, extruding out internal seeds, and cleaning the seeds by using the pure water;
(2) And (3) sterilization treatment: soaking the seeds in a 3-5% sodium hypochlorite solution for 14-16 minutes, and cleaning the seeds with pure water for later use;
(3) Preparing 6-benzylaminopurine treatment solution: dissolving 2.5-7.5 mg of 6-benzylaminopurine in 1mol/L hydrochloric acid solution, and diluting with pure water to 500 ml to prepare 5-15mg/L disposable 6-benzylaminopurine treatment solution;
(4) Seed pretreatment: completely immersing the sterilized eel grass seeds into the 6-benzylaminopurine treatment solution prepared in the step (3), placing the eel grass seeds in a thermostatic chamber for 22-26 hours, and then cleaning the eel grass seeds by using pure water for later use;
(5) Sowing: putting the pretreated eel grass seeds into a culture container which is filled with lake sediment and tap water and is kept still for 22-26 hours;
(6) Setting a culture environment: after sowing, putting the culture container into a constant-temperature chamber for germination;
(7) And (3) seedling growth: culturing in a thermostatic chamber for 50 days, replenishing water every week, and keeping the tape grass seedling submerged.
2. The method for promoting germination of seeds and seedling growth of submerged plant tape grass according to claim 1, wherein: the concentration of the 6-benzylaminopurine treatment solution in the step (3) is 10mg/L.
3. The method for promoting germination of seeds and seedling growth of submerged plant tape grass according to claim 1, wherein: in the steps (4) and (6), the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 1200-1800lx, and the light-dark ratio is 12/12 hours.
4. The method for promoting germination of seeds and seedling growth of submerged plant tape grass according to claim 1, wherein: in the step (4), the 6-benzylaminopurine treated and cleaned tape grass seeds are soaked in pure water for later use.
5. The method for promoting germination of seeds and seedling growth of submerged plant tape grass according to claim 1, wherein: the culture container in the step (5) is a plastic beaker, is filled with lake sediment and tap water, and is kept stand for 24 hours before seeding.
6. The method for promoting germination of seeds and seedling growth of submerged plant tape grass according to claim 1, wherein: in the step (5), the total nitrogen content of the lake sediment is 0.7-2.5g/kg, the total phosphorus content is 0.5-2.0g/kg, and the organic matter content is 4-9%.
7. The method for promoting germination of seeds and growth of seedlings of submerged plant tape grass according to claim 1, wherein: the seeds of the tape grass in the step (5) are placed in a single and uniform distribution mode.
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| CN1561687A (en) * | 2004-03-20 | 2005-01-12 | 中国科学院水生生物研究所 | Method for aseptic cultivating submerged plant |
| CN102172154A (en) * | 2011-02-25 | 2011-09-07 | 北京市水利科学研究所 | Method for culturing submerged plant seeds |
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| CN1561687A (en) * | 2004-03-20 | 2005-01-12 | 中国科学院水生生物研究所 | Method for aseptic cultivating submerged plant |
| CN102172154A (en) * | 2011-02-25 | 2011-09-07 | 北京市水利科学研究所 | Method for culturing submerged plant seeds |
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