CN115531257A

CN115531257A - Plant essential oil composition and skin care product added with same

Info

Publication number: CN115531257A
Application number: CN202211503956.4A
Authority: CN
Inventors: 赵纳; 丁振柱; 赵航
Original assignee: Cosmetics Biotechnology Shandong Co ltd
Current assignee: Cosmetics Biotechnology Shandong Co ltd
Priority date: 2022-11-29
Filing date: 2022-11-29
Publication date: 2022-12-30

Abstract

The invention discloses a plant essential oil composition and a skin care product added with the same, belonging to a preparation for dressing, wherein the plant essential oil composition comprises the following components in parts by weight: 0.5-1.9 parts of sandalwood essential oil; 0.5-1.9 parts of ginseng oil; 0.05-0.2 part of lime peel oil; 0.05-0.2 part of wild pueraria extract; 0.05-0.2 part of artemisia capillaris thunb extract; 0.1-0.5 parts of sophora flavescens root extract; 0.1-0.5 parts of glycyrrhiza inflata root extract; 0.2-0.7 parts of scutellaria root extract; 25-35 parts of chinaru seed oil; 5-8 parts of butanediol. The invention also discloses a skin care product added with the plant essential oil composition. Tests prove that the skin care product added with the plant essential oil composition can greatly improve the absorption effect of the hydroxypropyl tetrahydropyrane triol.

Description

Plant essential oil composition and skin care product added with same

Technical Field

The invention relates to the technical field of preparation products for dressing, in particular to a plant essential oil composition and a skin care product added with the composition.

Background

Vitriol (hydroxypropyl tetrahydropyrane triol) is a glycoprotein mixture derived from xylose, can stimulate the generation of skin aminoglucan (GAGs), and the molecular structure absorbs water, improves the compactness of the skin, can be used as a safe channel for guiding and repairing the molecular structure, promotes the synthesis of collagen, makes the skin more tough and elastic, improves neck fine wrinkles, and achieves the effect of delaying skin aging. The glass color is also a classic anti-aging and anti-wrinkle component in the cosmetic raw materials under the actual application and strong marketing of European elegant France.

The cosmetics containing the vitreous color cause are more and more on the market, the effect of adding the vitreous color cause particularly pays attention to the absorbance of the vitreous color cause (hydroxypropyl tetrahydropyrane triol), and important reference indexes of the absorbance are subcutaneous cumulative permeation quantity and diffusion percentage of the vitreous color cause in a certain time. At present, many products are available on the market, but the technology for improving the absorption degree of the vitreous color factor is not mentioned.

Therefore, the development of the plant essential oil composition and the skin care product added with the plant essential oil composition to improve the absorption degree of the vitronectin has urgent research value and also has good economic benefit and industrial application potential, which is the basis and the motivation for completing the invention.

Disclosure of Invention

The present inventors have conducted intensive studies to overcome the above-identified drawbacks of the prior art, and as a result, have completed the present invention after having made a great deal of creative efforts.

Specifically, the technical problems to be solved by the invention are as follows: provides a plant essential oil composition and a skin care product added with the composition, and aims to solve the technical problem that the absorption degree of vitronectin in the existing cosmetics is to be improved.

In order to solve the technical problems, the technical scheme of the invention is as follows:

in a first aspect, the invention provides a plant essential oil composition, which is prepared from the following components in parts by weight:

0.5-1.9 parts of sandalwood essential oil; 0.5-1.9 parts of ginseng oil; 0.05-0.2 part of lime peel oil; 0.05-0.2 part of wild pueraria extract; 0.05-0.2 part of artemisia capillaris extract; 0.1-0.5 parts of sophora flavescens root extract; 0.1-0.5 parts of glycyrrhiza inflata root extract; 0.2-0.7 parts of scutellaria root extract; 25-35 parts of chinaroot greenbrier seed oil; 5-8 parts of butanediol.

As a further improvement, the vegetable essential oil composition is prepared from the following components in parts by weight:

1.2 parts of sandalwood essential oil; 1.15 parts of ginseng oil; 0.1 part of lime peel oil; 0.1 part of wild pueraria extract; 0.1 part of artemisia capillaris extract; 0.35 part of sophora flavescens root extract; 0.38 portion of liquorice root extract; 0.46 part of scutellaria root extract; 30 parts of white chinlon seed oil; 6.5 parts of butanediol.

As a further improvement, the plant essential oil composition is prepared by a method comprising the following steps:

s1, respectively weighing half of sandalwood essential oil, ginseng oil and lime peel oil by weight; heating two thirds of the white chinlon oil to 35-45 ℃; under the conditions of the temperature system and ultrasonic stirring, sequentially dripping sandalwood essential oil, ginseng oil and lime peel oil into the white pond flower seed oil, and continuing ultrasonic stirring for 15-20 minutes after finishing dripping to obtain a mixed solution I;

s2, heating the mixed solution I by using a water bath at a heating rate of 3-5 ℃/min, and dropwise adding the rest of sandalwood essential oil, ginseng oil and lime peel oil into the mixed solution I in the sequence of the lime peel oil, the ginseng oil and the sandalwood essential oil, wherein the adding time is controlled to be 3-5 minutes; after the addition is finished, stopping heating and keeping the temperature unchanged, dropwise adding a certain amount of butanediol into the mixed solution, then carrying out ultrasonic stirring treatment for 10-15 minutes, and cooling to room temperature to obtain a mixed solution II;

s3, weighing a wild pueraria extract, an artemisia capillaris extract, a kuh-seng root extract, an oncomelania root extract and a scutellaria root extract, adding into a grinding instrument, and grinding for 1-3 minutes to obtain a mixture; uniformly dividing the rest amount of the meadowfoam seed oil into the mixture for three times, and immediately grinding for 1 minute after each addition to finally obtain a mixed material body;

and S4, uniformly mixing the mixed liquor II obtained in the step S2, the mixed material obtained in the step S3 and the residual butanediol under the condition of ultrasonic stirring to obtain the plant essential oil composition.

As a further improvement, in the step S2, the addition amount of the butanediol is two thirds of the total amount of the butanediol.

As a further improvement, in the step S1, the power of the ultrasonic agitation is 1000W; in the step S2, the power of ultrasonic stirring is 1500W; in the step S4, the power of the ultrasonic stirring is 1500W.

As a further improvement, in the step S3, the grinding rate of the grinding is 600-1200r/min.

On the other hand, the invention provides a skin care product added with the plant essential oil composition, which comprises the following raw materials in parts by weight based on 100 parts by weight of the skin care product:

36 parts of ginseng root water; 0.35 part of butanediol; 2.5 parts of cetearyl olive oleate; 0.5 part of sodium hyaluronate; 5 parts of squalane; 4 parts of shea butter extract; 4.5 parts of plant essential oil composition; 36 parts of a vitronectin compound; 2 parts of inositol; 2 parts of acetyl hexapeptide; 2 parts of behenyl alcohol; 1.75 parts of phytosterol; 0.75 part of ubiquinone; 0.5 part of p-hydroxyacetophenone; adenosine 0.5 parts; oligopeptide-1.35 parts; 0.26 part of ginsenoside; the balance of water.

As a further improvement, the boscalid complex is prepared from the following raw materials in parts by weight: ginseng root water: hydroxypropyl tetrahydropyran triol: butanediol = 65.

As a further improvement, the skin care product is prepared by adopting the following method:

1) Accurately weighing each component material in the formula components, and simultaneously cleaning and disinfecting production equipment;

2) Adding butanediol, cetearyl alcohol olive oleate and sodium hyaluronate into weighed water and ginseng root water, and heating to 70-85 ℃ to obtain a mixed solution A;

3) Mixing weighed squalane, shea butter extract and plant essential oil composition, and heating to 70-85 deg.C to obtain mixed solution B;

4) Adding the mixed solution A and the mixed solution B into a reaction tank in sequence, controlling the temperature to be 80 ℃, homogenizing and emulsifying for 3-5 minutes, then stirring at a low speed, defoaming, and cooling after defoaming is finished;

5) And when the temperature is reduced to 40 ℃, adding other materials, and continuously stirring until the materials are uniformly mixed to obtain the skin care product.

As a further improvement, in the step 4), the rotation speed of the low-speed stirring is 45-85 revolutions per minute.

After the technical scheme is adopted, the invention has the beneficial effects that:

according to the invention, reasonable essential oil components are firstly screened, and the plant essential oil composition is prepared by a specific process, wherein the sandalwood essential oil has the effects of promoting blood circulation, removing obstruction in channels, diminishing inflammation and protecting skin, and has the main effects of promoting the growth of skin cells, balancing and softening skin, and meanwhile, the emitted smell of the sandalwood essential oil can enable people to enter a relaxed state. Ginseng oil (Ginseng radix essential oil) has effects of promoting cutin degradation and delaying aging, and can accelerate generation of glucosaminoglycan in skin and activate skin. When the lime peel oil (namely, the lime essential oil) is used in aromatherapy, the lime peel oil is an essential oil which is not exuberant but can always bring pleasure to people; because it can bring happiness and warm feeling to the brain, can help people relax; but also can help the wrinkled skin to expel toxin. Other components, such as kudzu root extract, tarragon extract, kuh-seng root extract, glycyrrhiza inflate root extract, scutellaria root extract, are components that the inventors have screened in long-term practice; through a specific preparation method, various components can be fully wrapped and fused in a mixture system. Through the compounding of the raw materials and the preparation of the process, the synergistic effect is achieved among various components, and the effect of improving the absorption degree of the vitreous color is really achieved.

The invention also provides the application of the plant essential oil composition, and the skin care product containing the plant essential oil composition is prepared.

Drawings

Fig. 1 is a schematic view of the structure of a Franz cell diffusion cell used in the examples.

Detailed Description

The invention is further illustrated by the following specific examples. However, the use and purpose of these exemplary embodiments are only to exemplify the present invention, and do not constitute any limitation in any form to the actual scope of the present invention, and do not limit the scope of the present invention.

Example 1

A plant essential oil composition is prepared from the following components in parts by weight: 0.5 part of sandalwood essential oil; 0.5 part of ginseng oil; 0.05 part of lime peel oil; 0.05 part of wild pueraria extract; 0.05 part of artemisia capillaris extract; 0.1 part of sophora flavescens root extract; 0.1 part of glycyrrhiza inflata root extract; 0.2 part of scutellaria root extract; 25 parts of chinaru seed oil; 5 parts of butanediol.

The plant essential oil composition of the embodiment is prepared by adopting the method comprising the following steps:

s1, respectively weighing half of sandalwood essential oil, ginseng oil and lime peel oil by weight; heating two thirds of the white chinlon oil to 35 ℃; under the conditions of the temperature system and ultrasonic stirring, an FS-450N ultrasonic processor (Shanghai ultrasonic analysis instrument, inc.) is adopted, the power of ultrasonic stirring is 1000W, sandalwood essential oil, ginseng oil and lime peel oil are added into the white pond flower seed oil in a dropwise manner, and after the dropwise addition is finished, ultrasonic stirring treatment is continued for 15 minutes to obtain a mixed solution I;

s2, heating the mixed solution I by using water bath at a heating rate of 3 ℃/min, and dropwise adding the rest sandalwood essential oil, ginseng oil and lime peel oil into the mixed solution I in the sequence of the lime peel oil, the ginseng oil and the sandalwood essential oil, wherein the adding time is controlled to be 3 minutes; after the addition is finished, stopping heating and keeping the temperature unchanged, dropwise adding a certain amount of butanediol into the mixed solution, wherein the addition amount of the butanediol is two thirds of the total amount of the butanediol (namely 3.3 parts), then carrying out ultrasonic stirring treatment for 10 minutes, wherein the power of the ultrasonic stirring is 1500W, and cooling to room temperature to obtain a mixed solution II;

s3, weighing a wild kudzu root extract, an artemisia capillaries extract, a kuh-seng root extract, an oncomelania root extract and a scutellaria root extract, adding the weighed materials into a grinding instrument, and grinding for 3 minutes by adopting an ST-M200 tissue grinding instrument at a grinding speed of 600r/min to obtain a mixture; uniformly dividing the rest amount of the meadowfoam seed oil into the mixture for three times, immediately grinding the mixture for 1 minute at the grinding rate of 600r/min after each addition, and finally obtaining a mixed material body;

and S4, uniformly mixing the mixed liquid II obtained in the step S2, the mixed material obtained in the step S3 and the residual butanediol under the condition of ultrasonic stirring, wherein the power of the ultrasonic stirring is 1500W, so as to obtain the plant essential oil composition.

Example 2

The plant essential oil composition is prepared from the following components in parts by weight: 1.2 parts of sandalwood essential oil; 1.15 parts of ginseng oil; 0.1 part of lime peel oil; 0.1 part of wild pueraria extract; 0.1 part of artemisia capillaris thunb extract; 0.35 part of sophora flavescens root extract; 0.38 portion of glycyrrhiza inflata root extract; 0.46 part of scutellaria root extract; 30 parts of white Potentilla flower seed oil; 6.5 parts of butanediol.

The plant essential oil composition of the embodiment is prepared by adopting a method comprising the following steps of:

s1, respectively weighing half of sandalwood essential oil, ginseng oil and lime peel oil by weight; heating two thirds of the white chinlon oil to 40 ℃; under the conditions of the temperature system and ultrasonic stirring, an FS-450N ultrasonic processor (Shanghai ultrasonic analysis instrument, inc.) is adopted, the power of ultrasonic stirring is 1000W, sandalwood essential oil, ginseng oil and lime peel oil are added into the white pond flower seed oil in a dropwise manner, and after the dropwise addition is finished, ultrasonic stirring treatment is continued for 18 minutes to obtain a mixed solution I;

s2, heating the mixed solution I by using a water bath at a heating rate of 4 ℃/min, and dropwise adding the rest sandalwood essential oil, ginseng oil and lime peel oil into the mixed solution I in the sequence of the lime peel oil, the ginseng oil and the sandalwood essential oil, wherein the adding time is controlled to be 5 minutes; after the addition is finished, stopping heating and keeping the temperature unchanged, dropwise adding a certain amount of butanediol into the mixed solution, wherein the addition amount of the butanediol is two thirds of the total amount (namely 4.3 parts), then carrying out ultrasonic stirring treatment for 15 minutes, wherein the power of the ultrasonic stirring is 1500W, and cooling to room temperature to obtain a mixed solution II;

s3, weighing the kudzu root extract, the artemisia capillaries extract, the sophora flavescens root extract, the glycyrrhiza inflate root extract and the scutellaria baicalensis root extract, adding into a grinding instrument, grinding for 2 minutes by adopting an ST-M200 tissue grinding instrument, wherein the grinding speed is 1000r/min, and obtaining a mixture; uniformly dividing the rest chinaroot greenbrier seed oil into the mixture for three times, and immediately grinding the mixture for 1 minute at a grinding rate of 1000r/min after each addition to finally obtain a mixed material body;

Example 3

A plant essential oil composition is prepared from the following components in parts by weight: 1.9 parts of sandalwood essential oil; 1.9 parts of ginseng oil; 0.2 part of lime peel oil; 0.2 part of wild pueraria extract; 0.2 part of artemisia capillaris thunb extract; 0.5 part of sophora flavescens ait root extract; 0.5 part of glycyrrhiza inflata root extract; 0.7 part of scutellaria root extract; 35 parts of white chinlon seed oil; 8 parts of butanediol.

s1, respectively weighing half of sandalwood essential oil, ginseng oil and lime peel oil by weight; heating two thirds of the white Potentilla chinensis seed oil to 45 ℃; under the conditions of the temperature system and ultrasonic stirring, wherein an FS-450N ultrasonic processor (Shanghai ultrasonic analysis instrument, inc.) is adopted for ultrasonic stirring, the power of the ultrasonic stirring is 1000W, the sandalwood essential oil, the ginseng oil and the lime peel oil are sequentially dripped into the white pond flower seed oil, and after the dripping is finished, the ultrasonic stirring treatment is continued for 20 minutes to obtain a mixed solution I;

s2, heating the mixed solution I by using water bath at a heating rate of 5 ℃/min, and dropwise adding the rest sandalwood essential oil, ginseng oil and lime peel oil into the mixed solution I in the sequence of the lime peel oil, the ginseng oil and the sandalwood essential oil, wherein the adding time is controlled to be 5 minutes; after the addition is finished, stopping heating and keeping the temperature unchanged, then dropwise adding a certain amount of butanediol into the mixed solution, wherein the addition amount of the butanediol is two thirds of the total amount (namely 5.3 parts), then carrying out ultrasonic stirring treatment for 15 minutes, wherein the ultrasonic stirring power is 1500W, and cooling to room temperature to obtain a mixed solution II;

s3, weighing the kudzu root extract, the artemisia capillaries extract, the sophora flavescens root extract, the glycyrrhiza inflate root extract and the scutellaria baicalensis root extract, adding into a grinding instrument, grinding for 3 minutes by adopting an ST-M200 tissue grinding instrument, and obtaining a mixture at the grinding speed of 1200 r/min; uniformly dividing the rest amount of the meadowfoam seed oil into the mixture for three times, immediately grinding the mixture for 1 minute at the grinding rate of 1200r/min after each addition, and finally obtaining a mixed material body;

and S4, uniformly mixing the mixed liquid II obtained in the step S2, the mixed material obtained in the step S3 and the residual butanediol under the condition of ultrasonic stirring, wherein the power of the ultrasonic stirring is 1500W, and thus obtaining the plant essential oil composition.

Example 4

A skin care product added with a plant essential oil composition comprises the following raw materials in parts by weight, based on 100 parts by weight of the skin care product:

36 parts of ginseng root water; 0.35 part of butanediol; 2.5 parts of cetearyl olive oleate; 0.5 part of sodium hyaluronate; 5 parts of squalane; 4 parts of shea butter extract; 4.5 parts of plant essential oil composition; 36 parts of a vitronectin compound; 2 parts of inositol; 2 parts of acetyl hexapeptide; 2 parts of behenyl alcohol; 1.75 parts of phytosterol; 0.75 part of ubiquinone; 0.5 part of p-hydroxyacetophenone; adenosine 0.5 parts; oligopeptide-1.35 parts; 0.26 part of ginsenoside; the balance of water. The glass-color-factor compound is prepared from the following raw materials in parts by weight: ginseng root water: hydroxypropyl tetrahydropyran triol: butanediol = 65. The glass-color-factor compound can adopt a commercial product. Plant essential oil compositions were prepared using example 1.

The skin care product of the embodiment is prepared by the following method:

2) Adding butanediol, cetearyl olive oleate and sodium hyaluronate into the weighed water and ginseng root water, and heating to 70 ℃ to obtain a mixed solution A;

3) Mixing weighed squalane, shea butter extract and plant essential oil composition, and heating to 70 ℃ to obtain a mixed solution B;

4) Adding the mixed solution A and the mixed solution B into a reaction tank in sequence, controlling the temperature to be 80 ℃, homogenizing and emulsifying for 3 minutes, then stirring at a low speed, defoaming, wherein the rotating speed of the low-speed stirring is 45 revolutions per minute, and cooling after the defoaming is finished;

Example 5

36 parts of ginseng root water; 0.35 part of butanediol; 2.5 parts of cetearyl olive oleate; 0.5 part of sodium hyaluronate; 5 parts of squalane; 4 parts of shea butter extract; 4.5 parts of plant essential oil composition; 36 parts of a vitronectin compound; 2 parts of inositol; 2 parts of acetyl hexapeptide; 2 parts of behenyl alcohol; 1.75 parts of phytosterol; 0.75 part of ubiquinone; 0.5 part of p-hydroxyacetophenone; 0.5 part of adenosine; oligopeptide-1.35 parts; 0.26 part of ginsenoside; the balance of water. The bosity factor compound is prepared from the following raw materials in percentage by weight: ginseng root water: hydroxypropyl tetrahydropyran triol: butanediol = 65. The glass-ceramic complex can adopt a commercial product. Plant essential oil compositions were prepared using example 2.

The skin care product of the embodiment is prepared by the following method:

2) Adding butanediol, cetearyl olive oleate and sodium hyaluronate into the weighed water and ginseng root water, and heating to 80 ℃ to obtain a mixed solution A;

3) Mixing weighed squalane, shea butter extract and plant essential oil composition, and heating to 80 ℃ to obtain a mixed solution B;

4) Adding the mixed solution A and the mixed solution B into a reaction tank in sequence, controlling the temperature to be 80 ℃, homogenizing and emulsifying for 5 minutes, then stirring at a low speed, defoaming, wherein the rotating speed of the low-speed stirring is 60 revolutions per minute, and cooling after the defoaming is finished;

Example 6

A skin care product added with a plant essential oil composition comprises the following raw materials in parts by weight based on 100 parts by weight of the skin care product:

36 parts of ginseng root water; 0.35 part of butanediol; 2.5 parts of cetearyl olive oleate; 0.5 part of sodium hyaluronate; 5 parts of squalane; 4 parts of shea butter extract; 4.5 parts of plant essential oil composition; 36 parts of a vitronectin compound; 2 parts of inositol; 2 parts of acetyl hexapeptide; 2 parts of behenyl alcohol; 1.75 parts of phytosterol; 0.75 part of ubiquinone; 0.5 part of p-hydroxyacetophenone; 0.5 part of adenosine; oligopeptide-1.35 parts; 0.26 part of ginsenoside; the balance of water. The bosity factor compound is prepared from the following raw materials in percentage by weight: ginseng root water: hydroxypropyl tetrahydropyran triol: butanediol = 65. The glass-ceramic complex can adopt a commercial product. Plant essential oil compositions were prepared using example 3.

The skin care product of the embodiment is prepared by the following method:

2) Adding butanediol, cetearyl olive oleate and sodium hyaluronate into the weighed water and ginseng root water, and heating to 85 ℃ to obtain a mixed solution A;

3) Mixing weighed squalane, shea butter extract and plant essential oil composition, and heating to 85 ℃ to obtain a mixed solution B;

4) Adding the mixed solution A and the mixed solution B into a reaction tank in sequence, controlling the temperature to be 80 ℃, homogenizing and emulsifying for 4 minutes, then stirring at a low speed, defoaming, wherein the rotation speed of the low-speed stirring is 85 revolutions per minute, and cooling after the defoaming is finished;

Comparative example 1

The components and preparation process of example 5 were used with the following differences: in this comparative example, the plant essential oil composition was not added, and the same weight of ginseng root water as the plant essential oil composition was added, and the rest was the same.

Comparative example 2

The components and preparation process of example 5 were used with the following differences: in this comparative example, the plant essential oil composition was not added, but in step 3), the raw materials of the plant essential oil composition of example 2 were directly added in step 3) (not prepared by the process of example 2), mixed uniformly, and the rest was consistent.

Comparative example 3

The components and preparation process of example 5 were used with the following differences: in this comparative example, the added plant essential oil composition was different from that of example 2, and the rest was the same.

The added plant essential oil composition differs from example 2 in that: the sandalwood essential oil is not contained, the other components are consistent with the process, and the ginseng root water with the same weight is added in the production.

Comparative example 4

The added plant essential oil composition differs from example 2 in that: the sour orange peel oil and the scutellaria root extract are not contained, the other components are consistent with the process, and the ginseng root water with the same weight is added in the production.

Comparative example 5

The added plant essential oil composition differs from example 2 in that: the sandalwood essential oil is replaced by lavender essential oil, and the other components and the process are consistent.

Comparative example 6

The added plant essential oil composition differs from example 2 in that: 0.2 part by weight of lavender essential oil is additionally added, the other components are consistent with the process, and the ginseng root water with the same weight is reduced in the production.

Comparative example 7

The added plant essential oil composition differs from example 2 in that: the white chinaroot seed oil is replaced by the nut oil, and the other components and the process are consistent.

Evaluation of anti-wrinkle efficacy of skin Care products (assisted detection by Guangdong Lichuang detection technology service Co., ltd.)

The skin care product prepared in example 5 (100 g) was evaluated for anti-wrinkle efficacy according to the laboratory procedure (LC-WI-HZ-174 free radical scavenging test instructions 1.2) as follows:

1. purpose and basis of experiments

External and body factors can cause the generation of free radicals, which have extremely strong oxidation capacity, attack any biological molecules through oxidation reaction, and generate various oxidation reactions with macromolecular substances such as lipid, saccharide, protein, deoxyribonucleic acid and the like to cause oxidative damage such as denaturation, crosslinking, fracture and the like, further cause the damage of cell structures and functions, the damage of body tissues and the pathological changes of organs, and appear as aging, wrinkles and the like on the skin. The 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) reagent, a stable long-lived radical, absorbs less light when the test sample is in the presence of a free radical scavenger. The ability of the test sample to scavenge free radicals, i.e., the magnitude of antioxidant activity, can thus be evaluated.

2. Correlation of test methods

The method is an in vitro method, and is suitable for cosmetics with the alleged effects of anti-aging, anti-wrinkle and anti-oxidation activity. And displaying relevant literature data. When the test sample has the free radical scavenger, the degree of the decolorization of the DPPH solution is in a linear relationship with the free radical scavenging ability of the free radical scavenger, so that the free radical scavenging ability, i.e., the magnitude of the antioxidant activity, of the test sample can be evaluated. Comparing the result of DPPH free radical clearance test between the test sample and the blank control group through test design. If the clearance of the test sample is better (higher) than that of the blank control group and the statistical difference P value is less than 0.05, the test sample can be considered to have the efficacy of resisting wrinkles.

3. Test items

Test indexes	Criteria for determination
		DPPH radical clearance rate	Calculating the DPPH free radical clearance rate before and after adding the test substance, wherein the statistical difference P value is less than 0.05

4. Test method

Weighing sample → pretreating the sample to be tested → adding free radical DPPH, preparing multi-tube sample, shaking, standing at room temperature for a while, testing on machine to determine light absorption value → recording data, and calculating clearance and statistical difference P before and after adding the test substance.

5. Test results (see Table below)

Test items	Blank control group	Test sample set	P value	Significance determination
					DPPH radical scavenging (%) average value	0.35	96.93	P＜0.05	Is remarkable in that

6. Conclusion

The clearance of the test sample is superior to (higher than) that of a blank control group, the statistical difference P value is less than 0.05, and the difference is remarkable, so that the test sample has the anti-wrinkle effect.

The skin care products prepared in examples 4 and 6 were also evaluated for anti-wrinkle efficacy, and the effect was similar to that of example 5, indicating that the skin care products provided by the present invention have relatively good anti-wrinkle efficacy.

Vitronectin in vitro transdermal absorption assay (assisted by Shanghai micro-Spectroscopy, science and technology group, inc.).

1. Purpose of testing

The test is based on a piglet skin-Franz cell diffusion cell system, and Franz cell diffusion cell permeability experiments are carried out on different samples by the back skin of a piglet to investigate the transdermal capacity of the samples on the back skin of the piglet in vitro. The in vitro transdermal behavior of the cell is described by subsequently measuring the content of the vitreous color factor in the diffusion cell at different time points.

2. Materials and samples

2.1 Test model and test system

The test selects the pigskin as a test model, and selects a pigskin-Franz cell diffusion cell system as a test system.

2.2 Reagent

Physiological saline.

2.3 Device

TPY-2 percutaneous absorption diffusion instrument (Shanghai yellow sea medical testing instrument Co., ltd.), franz cell diffusion cell (Shanghai yellow sea medical testing instrument Co., ltd.), and KQ3200E type ultrasonic cleaning instrument (Kunshanshumei).

2.4 Reference method

The experiment is according to the in vitro test method of the skin absorption of the GBT27818-2011 chemical.

3. Testing device

The TPY-2 type transdermal diffusion tester structurally comprises a constant-temperature water bath tank, a control box, an operating platform, a Franz cell diffusion pool, a liquid supplementing pipe and a connecting pipe. The upper part of the inner bottle of the Franz cell diffusion pool is provided with a liquid supplementing port control box which is respectively connected with a constant temperature water bath box and an operation platform through electric wires in a circuit mode, the constant temperature water bath box is communicated with the outer bottle of the Franz cell diffusion pool through a connecting pipe and is communicated with the liquid supplementing port of the Franz cell diffusion pool through the connecting pipe, and the Franz cell diffusion pool is placed on the operation platform. The device can be used for simultaneously carrying out multiple transdermal experiments, has no mutual interference and no bubble phenomenon, and has a receiving chamber with a volume of 8.0 mL and a diffusion area of 3.14 cm ² . Referring to fig. 1, a franz cell diffusion cell is formed by joining an upper cylindrical glass tube and a lower cylindrical glass tube, and is clamped by an elastic clamp 2, and a patch 1 clamped between the two cylindrical glass tubes divides the upper and lower cylindrical glass tubes into two chambers, wherein the patch is a skin or a skin substitute. The upper chamber is a diffusion chamber 3, the lower chamber is a receiving chamber 4, the upper part of the diffusion chamber is covered with a transparent glass plate 5The right part of the receiving chamber is connected with a sampling tube 6, a sampling needle 8 penetrates through the sampling tube for sample introduction, sampling and bubble exhaust, and the bottom of the receiving chamber is provided with a magnetic stirrer 7. The fluid infusion port is not shown, and during testing, the infused sample fluid 9 is on the patch 1 and the permeate fluid 10 is in the receiving chamber 4.

4. Grouping of test products

Skin care products prepared in example 4, example 5, example 6.

Skin care products prepared according to comparative examples 1 to 7.

Commercially available products containing vitronectin (ingredients of water, polydimethylsiloxane, hydroxypropyl tetrahydropyran triol, propylene glycol, cyclohexasiloxane, isohexadecane, glycerol, synthetic wax, polydimethylsiloxane PEG-10/15 cross-linked polymer, aluminum starch octenyl succinate, lauryl PEG-9 polydimethylsiloxyethyl polydimethylsiloxane, CI 77163, magnesium sulfate, phenoxyethanol, ethylhexyl hydroxystearate, acrylonitrile/methyl methacrylate/vinylidene chloride copolymer, perfume, disodium EDTA, VACCINIUM MYRTILLUS (Vaccinium MYRTILLUS) fruit extract, dipotassium glycyrrhizinate, and sodium hyaluronate).

5. Transdermal absorption assay procedure

(1) Adding a receiving liquid into the receiving chamber: 7.0 mL of receiving solution (physiological saline) was aspirated by a pipette and injected into the receiving chamber, and a magnetic stirrer 7 was placed in the receiving chamber.

(2) Assembling and fixing the test model: the pigskin was held between the diffusion chamber and the receiving chamber of a Franz cell diffusion cell with the stratum corneum facing the diffusion chamber and the dermis facing the receiving chamber. After the pigskin is fixed, 1.0 mL of receiving solution (normal saline) is added into the sampling tube by a pipette according to the liquid height of the sampling tube, so that the corium layer of the pigskin is tightly contacted with the receiving solution, and the total volume of the receiving solution is 8.0 mL.

(3) The Franz cell diffusion cell was fixed in a percutaneous absorption diffusion apparatus, and an electromagnetic stirrer was started to stir at 300 rpm, and a constant temperature of (32. + -. 1) ° C was maintained.

(4) Sampling: after the temperature of the water bath of the diffusion instrument is constant, the sample is loaded, and 400 mu L of sample is absorbed by a pipette and added to the surface of the pigskin.

(5) Collecting subcutaneous samples: 2.0 mL of the receiving solution was withdrawn through a Peek tube connected pipette at

time points

2, 8, and 24h, respectively, and placed in a 2.0 mL EP tube.

(6) Treatment of the non-permeated part on the skin: 24 And h, after the permeation of the sample is finished, sucking the physiological saline solution by using a liquid transfer gun, repeatedly blowing and cleaning the surface of the pigskin for many times, cleaning for 3 times, and fixing the volume to 2.0 mL.

(7) Treatment of residual parts in skins: 24h (sample) after permeation is finished, the pigskin is cut into pieces and placed in a 2.0 mL EP tube, the volume is adjusted to 2.0 mL by using normal saline, and the ultrasonic treatment is carried out for 30 minutes.

(8) Samples from each time point were analyzed for glass color content by High Performance Liquid Chromatography (HPLC).

6. Statistics and analysis

6.1 Cumulative amount of penetration

Q=Cn×V+∑Ci×V0 (i=1···n-1) ，

In the formula: q: cumulative permeation, V: volume of receiving solution in receiving chamber, 8.0 ml, v0: volume per sample, 2.0 mL, ci: concentration of drug in the receiving solution at the time of sampling 1 to n-1 times, cn: the sample concentration measured at the nth sampling point.

6.2 Percentage of diffusion

P=Q/P0×100%

In the formula: p: percent diffusion, Q: cumulative permeation of the sample at each time point in the receiving chamber, P0: initial loading of sample in the diffusion chamber.

7. Test results

7.1 Initial vitronectin content

After detection, the content of the vitronectin in the products containing the vitronectin in the examples 4-6, the comparative examples 1-7 and the commercial products containing the vitronectin is basically the same.

7.2 Cumulative amount of penetration

The cumulative permeation of the samples at different time points was calculated according to the 6.1 calculation formula, and the results are shown in the following table.

Note: calculating the accumulated permeation amount of the filter medium at each time point according to the formula Q = [ Cn × V0+ ∑ Ci × Vi ]

V0: volume of receiving solution in receiving chamber, 8.0 ml: volume per sample, 2.0 mL, ci: concentration of drug in the receiving solution at 1 st to n-1 th sampling, cn: the sample concentration measured at the nth sampling point.

7.3 Percent diffusion results

The percentage diffusion of the samples at different time points was calculated according to the formula 6.2 and the results are given in the table below.

Note: the percentage of diffusion at each time point was calculated according to the formula [ P = Q/P0 × 100% ]

Q is the sample content obtained at each time point in the receiving chamber, and P0 is the sample loading amount (loading amount = loading volume × sample concentration) of the sample in the diffusion chamber, wherein P0 at different time points has a slight error due to the data read by the instrument, but does not affect the result, and the error is negligible.

From the above results it is evident that:

the skin care products of examples 4-6 had significantly higher cumulative permeation amounts and diffusion percentages at 2h, 8h, and 24h than the other groups.

Comparative example 1, the commercial product which does not contain the plant essential oil composition of the invention, has obviously low cumulative penetration amount and diffusion percentage in 2h, 8h and 24h, which indicates that whether the plant essential oil composition is added or not has great influence on the penetration amount and diffusion percentage of the vitronectin.

Comparative example 2 raw materials of the plant essential oil composition were directly added in step 3), but the plant essential oil composition was not prepared by a specific process, and the cumulative permeation amount and the diffusion percentage were also lower in 2h, 8h and 24h, indicating that the plant essential oil composition prepared by a specific process can exert better effect of promoting increase of the vitreous color permeation amount and the diffusion percentage.

The comparative examples 3, 4 and 6 are that one or more components in the plant essential oil composition are deleted or added, and the cumulative permeation amount and the diffusion percentage of the plant essential oil composition are greatly reduced in 2h, 8h and 24 h. The components of the plant essential oil composition are synergistic, and if the components are simply deleted and added, the effect is poor.

Comparative examples 5 and 7 are simple replacement of certain components in the plant essential oil composition, and the cumulative permeation amount and the diffusion percentage of the plant essential oil composition are greatly reduced in 2h, 8h and 24 h. The components of the plant essential oil composition are synergistic, and if the components are simply replaced, the effect is poor.

For structural devices known to those skilled in the art in the embodiments, detailed descriptions are omitted, and it is believed that those skilled in the art can know through searching and finally implement the technical solutions described in the present invention.

It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Moreover, it should also be understood that various alterations, modifications and/or variations can be made to the present invention by those skilled in the art after reading the technical content of the present invention, and all such equivalents fall within the scope of protection defined by the claims appended to this application.

Claims

1. The plant essential oil composition is characterized by being prepared from the following components in parts by weight:

0.5-1.9 parts of sandalwood essential oil; 0.5-1.9 parts of ginseng oil; 0.05-0.2 part of lime peel oil; 0.05-0.2 part of wild pueraria extract; 0.05-0.2 part of artemisia capillaris thunb extract; 0.1-0.5 parts of sophora flavescens root extract; 0.1-0.5 parts of glycyrrhiza inflata root extract; 0.2-0.7 parts of scutellaria root extract; 25-35 parts of chinaroot greenbrier seed oil; 5-8 parts of butanediol.

2. The plant essential oil composition as claimed in claim 1, which is prepared from the following components in parts by weight:

1.2 parts of sandalwood essential oil; 1.15 parts of ginseng oil; 0.1 part of lime peel oil; 0.1 part of wild pueraria extract; 0.1 part of artemisia capillaris thunb extract; 0.35 part of sophora flavescens root extract; 0.38 portion of liquorice root extract; 0.46 part of scutellaria root extract; 30 parts of white chinlon seed oil; 6.5 parts of butanediol.

3. A plant essential oil composition according to claim 1 or 2, which is prepared by a process comprising the steps of:

s2, heating the mixed solution I by using a water bath, wherein the heating rate is 3-5 ℃/min, and dropwise adding the rest of sandalwood essential oil, ginseng oil and lime peel oil into the mixed solution I in the sequence of the lime peel oil, the ginseng oil and the sandalwood essential oil, wherein the adding time is controlled to be 3-5 minutes; after the addition is finished, stopping heating, keeping the temperature unchanged, dropwise adding a certain amount of butanediol into the mixed solution, performing ultrasonic stirring treatment for 10-15 minutes, and cooling to room temperature to obtain a mixed solution II;

s3, weighing the kudzu root extract, the artemisia capillaries extract, the sophora flavescens root extract, the glycyrrhiza inflata root extract and the scutellaria baicalensis root extract, adding into a grinding instrument, and grinding for 1-3 minutes to obtain a mixture; uniformly dividing the rest amount of the meadowfoam seed oil into the mixture for three times, and immediately grinding for 1 minute after each addition to finally obtain a mixed material body;

4. A plant essential oil composition according to claim 3, wherein in step S2, the butylene glycol is added in an amount of two thirds of the total amount.

5. The plant essential oil composition as claimed in claim 4, wherein in the step S1, the power of the ultrasonic agitation is 1000W; in the step S2, the power of ultrasonic stirring is 1500W; in the step S4, the power of the ultrasonic stirring is 1500W.

6. A plant essential oil composition according to claim 5, wherein in step S3, the milling is carried out at a rate of 600 to 1200r/min.

7. A skin care product added with plant essential oil composition is characterized by comprising 4.5 parts by weight of the plant essential oil composition according to any one of claims 1 to 6 based on 100 parts by weight of the total weight of the skin care product;

the feed also comprises the following raw materials in parts by weight:

36 parts of ginseng root water; 0.35 part of butanediol; 2.5 parts of cetearyl olive oleate; 0.5 part of sodium hyaluronate; 5 parts of squalane; 4 parts of shea butter extract; 36 parts of a vitronectin compound; 2 parts of inositol; 2 parts of acetyl hexapeptide; 2 parts of behenyl alcohol; 1.75 parts of phytosterol; 0.75 part of ubiquinone; 0.5 part of p-hydroxyacetophenone; adenosine 0.5 parts; oligopeptide-1.35 parts; 0.26 part of ginsenoside; the balance of water.

8. The skin care product added with the plant essential oil composition as claimed in claim 7, wherein the boswellia color composition is prepared from the following raw materials in parts by weight:

ginseng root water: hydroxypropyl tetrahydropyran triol: butanediol = 65.

9. The skin care product added with the plant essential oil composition as claimed in claim 8, which is prepared by the following method:

3) Mixing the weighed squalane, shea butter extract and plant essential oil composition, and heating to 70-85 ℃ to obtain a mixed solution B;

10. The skin care product with the addition of the plant essential oil composition as claimed in claim 9, wherein in the step 4), the rotation speed of the low-speed stirring is 45-85 rpm.