CN115478001B

CN115478001B - Device for subpackaging strain culture medium

Info

Publication number: CN115478001B
Application number: CN202211329138.7A
Authority: CN
Inventors: 王海霞; 李冬; 杜涵; 李阿波; 沈甜; 李晓龙; 冯学梅; 牛锐敏; 黄岳; 贾永华
Original assignee: Institute of Horticulture of Ningxia Academy of Agriculture and Forestry Sciences
Current assignee: Institute of Horticulture of Ningxia Academy of Agriculture and Forestry Sciences
Priority date: 2022-10-27
Filing date: 2022-10-27
Publication date: 2023-03-17
Anticipated expiration: 2042-10-27
Also published as: CN115478001A

Abstract

The invention discloses a device for subpackaging strain culture media, and belongs to the technical field of strain culture equipment. This device of partial shipment of bacterial culture medium includes support and revolving rack, still includes: the device comprises a plurality of pipe clamps, a solid culture medium and a liquid storage tank, wherein each pipe clamp is used for clamping a test tube, and a solid culture medium is arranged in each test tube; the vertical frame is arranged on the support and provided with a sliding groove in the vertical direction; the screw rod is connected with the vertical frame bearing and is connected with a first power device; the screw sleeve is in threaded connection with the screw rod, and the outer wall of the screw sleeve is in sliding connection with the sliding groove; the annular hot air pipe is connected with the nut sleeve, the annular hot air pipe is communicated with a hot air supply device, a plurality of air nozzles are arranged on the inner wall of the annular hot air pipe, and the inner wall of the annular hot air pipe can be sleeved outside the test tube on the tube clamp. The device for subpackaging the strain culture medium can prevent sundry bacteria from entering the test tube to cause sundry bacteria pollution when the strain subpackaging operation is carried out, so that the purity and the activity of bacterial colonies in the solid culture medium in the test tube are improved.

Description

Device of bacterial culture medium partial shipment

Technical Field

The invention relates to the technical field of strain culture equipment, in particular to a device for subpackaging strain culture media.

Background

The strain preservation refers to the preservation of various original characteristics and physiological activities of strains, and the basic principle is to make the life activities of microorganisms in a semi-permanent dormant state. The colony existing in nature is separated to obtain wild strains of different strains, so that the wild strains are not polluted by other strains and can survive for a long time, and the original various characteristics, physiological activity and genetic characters of the strains are maintained. When the strains are preserved, some strain culture medium split charging equipment is needed. When the strains are subpackaged, the interference of mixed bacteria is avoided as much as possible, and the purity of the original flora is kept.

Some existing devices for subpackaging strain culture media generally comprise a support, a rotating frame and a plurality of test tubes filled with solid culture media, wherein the plurality of test tubes filled with the solid culture media are arranged on the rotating frame, and the rotating frame is connected with a support bearing. When using, the user is through rotating the revolving rack to rotate the test tube of difference to the work position, the user plants the bacterial to the culture medium in the test tube in, thereby accomplishes the partial shipment of bacterial.

But the device of current bacterial culture medium partial shipment, when carrying out bacterial culture medium partial shipment, lack the function that carries out pasteurization to the mouth of pipe of the test tube that is equipped with solid medium to when carrying out bacterial partial shipment operation, be difficult to prevent that miscellaneous fungus from getting into and causing miscellaneous fungus pollution in the test tube, thereby be difficult to guarantee the purity of the interior bacterial colony of solid medium in the test tube.

Disclosure of Invention

The invention aims to overcome the problems in the prior art, and provides a device for subpackaging a strain culture medium, which can assist in carrying out high-temperature sterilization on a pipe orifice of a test tube filled with a solid culture medium during subpackaging of the strain culture medium, so that the phenomenon that mixed bacteria enter the test tube to cause mixed bacteria pollution during subpackaging operation of the strain is prevented, and the purity and the activity of bacterial colonies in the solid culture medium in the test tube are improved.

The invention provides a device for subpackaging strain culture mediums, which comprises a bracket and a rotating frame, and also comprises:

the pipe clamps are circumferentially arranged on the rotating frame, each pipe clamp clamps a test tube, and a solid culture medium is arranged in each test tube;

the vertical frame is arranged on the support and is provided with a sliding groove in the vertical direction;

the lead screw is vertically arranged, is connected with the vertical frame bearing, and is connected with a first power device which is electrically connected with a controller;

the screw sleeve is in threaded connection with the screw rod, and the outer wall of the screw sleeve is in sliding connection with the sliding groove;

the annular hot-blast main is connected with the nut sleeve, the annular hot-blast main is communicated with a hot air supply device, the inner wall of the annular hot-blast main is provided with a plurality of air nozzles, and the inner wall of the annular hot-blast main can be sleeved outside the test tube on the tube clamp.

Preferably, the pipe clamp is pivotally connected with the rotating frame, a sliding block is arranged on one side of the bottom end of the support, the sliding block is provided with an arc-shaped guide surface, the arc-shaped guide surface is abutted to the side wall of the bottom end of the pipe clamp, and the sliding block is used for driving the bottom end of the pipe clamp to axially deflect towards the rotating frame.

Preferably, a maintaining frame is further arranged between the pipe clamp and the rotating frame, the maintaining frame is fixedly connected with the pipe clamp, a rotating shaft is arranged on the side wall of the maintaining frame, the rotating shaft is pivotally connected with the rotating frame, a coil spring is arranged on the rotating frame, one end of the coil spring is connected with the rotating frame, and the other end of the coil spring is connected with the rotating shaft.

Preferably, a clamping block is arranged on the rotating shaft, a stop block is arranged on the rotating frame, the stop block can be clamped with the clamping block, and the clamping block and the stop block are used for limiting the rotating angle of the rotating shaft.

Preferably, a temperature sensor is arranged on the nut sleeve, the temperature sensor is electrically connected with the controller, the controller is electrically connected with the hot air supply device, the temperature sensor is used for detecting a real-time temperature value of the annular hot air pipe, a preset temperature value is preset in the controller, the controller is used for comparing the real-time temperature value with the preset temperature value, and when the real-time temperature value is larger than the preset temperature value, the controller controls the hot air supply device to stop acting.

Preferably, the rotating frame is also electrically connected with a second power device, and the second power device is electrically connected with the controller.

Preferably, the pipe clamp is provided with a circular truncated cone hole, the test tube is inserted into the circular truncated cone hole, and a rubber pad is arranged between the circular truncated cone hole and the test tube.

Preferably, the side wall of the circular truncated cone hole is provided with a certain taper.

Compared with the prior art, the invention has the beneficial effects that: the device for subpackaging the strain culture medium can assist in carrying out high-temperature sterilization on the pipe orifice of the test tube filled with the solid culture medium during subpackaging of the strain culture medium, so that the phenomenon that mixed bacteria enter the test tube to cause mixed bacteria pollution during subpackaging operation of the strain is prevented, and the purity and the activity of bacterial colonies in the solid culture medium in the test tube are improved.

Through setting up the slider, can the person of facilitating the use carry out the bacterial and plant the operation to promote the convenience of bacterial partial shipment. Through setting up the coil spring, can prevent that the pipe clamp of activity from causing test tube and support or grudging post to bump, avoid the test tube impaired. Through setting up fixture block and dog, can restrict the pipe clamp and rotate to further restrict the pipe clamp and rock, avoid the test tube impaired. Through setting up temperature sensor, can prevent that test tube wall temperature is too high, lead to the in vitro solid medium to melt. Through setting up second power device, can promote the degree of automation of this device, promote the use convenience of whole device. Through setting up the round platform hole, can the person of facilitating the use presss from both sides the test tube on the pipe clamp, can also promote the firm nature that the pipe clamp pressed from both sides tight test tube. Through setting up the lateral wall with the round platform hole to have certain tapering, can further promote the tight firm degree of test tube clamp, prevent the test tube landing.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 isbase:Sub>A schematic view of the structure of the A-A surface of the present invention.

Description of reference numerals:

101. the test tube testing device comprises a support, 102, a rotating frame, 103, a pipe clamp, 104, a test tube, 105, a vertical frame, 106, a sliding groove, 107, a screw rod, 108, a first power device, 109, a nut sleeve, 110, an annular hot air pipe, 111, an air nozzle, 201, a sliding block, 202, an arc-shaped guide surface, 301, a maintaining frame, 302, a rotating shaft, 303, a coil spring, 401, a clamping block, 402, a stop, 5, a temperature sensor, 6, a second power device, 701, a circular table hole and 702 rubber pads.

Detailed Description

The following detailed description of the present invention is provided in conjunction with the accompanying fig. 1 and 2, but it should be understood that the scope of the present invention is not limited to the specific embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

Example 1:

as shown in fig. 1 and 2, the apparatus for subpackaging strain culture medium provided by the present invention comprises a support 101 and a rotating frame 102, and further comprises: the device comprises a plurality of pipe clamps 103, a vertical frame 105, a screw rod 107, a nut sleeve 109 and an annular hot air pipe 110, wherein the pipe clamps 103 are circumferentially arranged on the rotating frame 102, each pipe clamp 103 clamps a test tube 104, and a solid culture medium is arranged in each test tube 104; the vertical frame 105 is arranged on the support 101, and the vertical frame 105 is provided with a sliding groove 106 in the vertical direction; the screw rod 107 is vertically arranged, the screw rod 107 is in bearing connection with the vertical frame 105, the screw rod 107 is connected with a first power device 108, and the first power device 108 is electrically connected with a controller; the screw sleeve 109 is in threaded connection with the screw rod 107, and the outer wall of the screw sleeve 109 is in sliding connection with the sliding groove 106; the annular hot air pipe 110 is connected with the nut sleeve 109, the annular hot air pipe 110 is communicated with a hot air supply device, a plurality of air nozzles 111 are arranged on the inner wall of the annular hot air pipe 110, and the inner wall of the annular hot air pipe 110 can be sleeved outside the test tube 104 on the tube clamp 103.

The working principle of example 1 will now be briefly described:

when the device is used, a plurality of test tubes 104 filled with solid culture media are respectively inserted into different tube clamps 103, the wooden plugs are uncovered, strains are planted in the solid culture media of the test tubes 104, and the wooden plugs are covered after the strains are planted. The split charging of the strains is accomplished by a plurality of test tubes 104 on the rotating frame 102. Then, the test tube 104 is rotated to a position below the annular hot air pipe 110, and then high-temperature hot air is introduced into the annular hot air pipe 110 by the hot air supply device and is ejected from the nozzle of the inner wall of the annular hot air pipe 110. At this time, the controller controls the first power device 108 to rotate reversely, the first power device 108 drives the screw rod 107 to rotate reversely, the screw rod 107 drives the nut sleeve 109 to move downwards along the sliding groove 106 on the stand 105, so as to drive the annular hot air pipe 110 to move downwards, and the annular hot air pipe 110 is sleeved outside the test tube 104 and moves downwards along the axial direction of the test tube 104. Meanwhile, the high-temperature hot air ejected from the nozzle is sprayed to the outer wall of the test tube 104, thereby destroying the bacteria on the outer wall of the nozzle of the test tube 104. When the annular hot air pipe 110 moves to the middle of the test tube 104, the controller controls the first power device 108 to rotate forward, and the first power device 108 drives the screw rod 107 to rotate forward, so that the annular hot air pipe 110 is driven to move upward. Reciprocating like this to furthest kills the bacterial contamination of test tube 104 mouth totally, thereby promotes the purity and the activity of the interior bacterial colony of solid medium of test tube 104.

The device for subpackaging the strain culture medium can assist in carrying out high-temperature sterilization on the pipe orifice of the test tube 104 filled with the solid culture medium during subpackaging of the strain culture medium, so that the phenomenon that mixed bacteria enter the test tube 104 to cause mixed bacteria pollution during subpackaging operation of the strain is prevented, and the purity and the activity of bacterial colonies in the solid culture medium in the test tube 104 are improved.

Example 2:

on the basis of embodiment 1, for the convenience of the user to carry out the bacterial planting operation, thereby promoting the convenience of bacterial subpackaging.

As shown in fig. 1 and 2, the pipe clamp 103 is pivotally connected to the rotating frame 102, a sliding block 201 is disposed on one side of the bottom end of the support 101, the sliding block 201 is provided with an arc-shaped guide surface 202, the arc-shaped guide surface 202 abuts against a side wall of the bottom end of the pipe clamp 103, and the sliding block 201 is used for driving the bottom end of the pipe clamp 103 to deflect axially towards the rotating frame 102.

When carrying out the bacterial planting, through rotating the revolving rack 102, when revolving rack 102 rotates the bottom and the slider 201 butt that load the pipe clamp 103 of the test tube 104 of planting the bacterial in advance, under the effect of the arc spigot surface 202 of slider 201, can crowd the axial to revolving rack 102 with pipe clamp 103 bottom, thereby make the top of pipe clamp 103 to the direction skew of keeping away from revolving rack 102, drive the test tube 104 on the pipe clamp 103 to the direction skew of keeping away from revolving rack 102, until test tube 104 skew predetermined angle, thereby the person of facilitating the use carries out the bacterial planting operation, thereby promote the convenience of bacterial partial shipment.

As a preferable scheme, as shown in fig. 1 and 2, a maintaining frame 301 is further disposed between the pipe clamp 103 and the rotating frame 102, the maintaining frame 301 is fixedly connected to the pipe clamp 103, a rotating shaft 302 is disposed on a side wall of the maintaining frame 301, the rotating shaft 302 is pivotally connected to the rotating frame 102, a coil spring 303 is disposed on the rotating frame 102, one end of the coil spring 303 is connected to the rotating frame 102, and the other end of the coil spring 303 is connected to the rotating shaft 302. Through setting up coil spring 303, under the effect of coil spring 303 elastic force, can prevent to make pipe clamp 103 on the revolving rack 102 appear rocking when revolving rack 102 rotates to prevent that movable pipe clamp 103 from causing test tube 104 and support 101 or grudging post 105 to collide, avoid test tube 104 impaired.

As a preferable scheme, as shown in fig. 2, a latch 401 is disposed on the rotating shaft 302, a stopper 402 is disposed on the rotating rack 102, the stopper 402 can be engaged with the latch 401, and the latch 401 and the stopper 402 are used for limiting the rotating angle of the rotating shaft 302. By arranging the fixture block 401 and the stopper 402, when the slider 201 is not in contact with the pipe clamp 103, the rotation of the pipe clamp 103 is limited by the clamping action of the fixture block 401 and the stopper 402, so that the shaking of the pipe clamp 103 is further limited, and the test tube 104 is prevented from being damaged.

As a preferable scheme, as shown in fig. 1, a temperature sensor 5 is arranged on the nut sleeve 109, the temperature sensor 5 is electrically connected to the controller, the controller is electrically connected to the hot air supply device, the temperature sensor 5 is configured to detect a real-time temperature value of the annular hot air pipe 110, a predetermined temperature value is preset in the controller, the controller is configured to compare the real-time temperature value with the predetermined temperature value, and when the real-time temperature value is greater than the predetermined temperature value, the controller controls the hot air supply device to stop operating. Through setting up temperature sensor 5, utilize temperature sensor 5 to detect the real-time temperature value of annular hot-blast main 110, when the real-time temperature value of annular hot-blast main 110 was too high, when both real-time temperature value exceeded predetermined temperature value, hot-blast feeding device of controller control stopped providing high temperature hot-blast to in the annular hot-blast main 110 to prevent that test tube 104 wall temperature is too high, lead to the interior solid medium of test tube 104 to melt.

Preferably, as shown in fig. 2, the rotating frame 102 is further electrically connected with a second power device 6, and the second power device 6 is electrically connected with the controller. Through setting up second power device 6, utilize second power device 6 drive revolving rack 102 to rotate, can promote the degree of automation of this device, promote the use convenience of whole device.

Preferably, as shown in fig. 1, the tube clamp 103 is provided with a circular truncated cone hole 701, the test tube 104 is inserted into the circular truncated cone hole 701, and a rubber pad 702 is arranged between the circular truncated cone hole 701 and the test tube 104. Through setting up round platform hole 701 to set up rubber pad 702 in round platform hole 701, can make things convenient for the user to adorn the clamp test tube 104 on pipe clamp 103, can also promote the firm nature that pipe clamp 103 presss from both sides tight test tube 104.

Preferably, as shown in fig. 1, the sidewall of the circular truncated cone hole 701 has a certain taper. Through setting up the lateral wall with circular platform hole 701 to have certain tapering, can further promote the firm degree that test tube 104 presss from both sides 103 tightly, prevent that test tube 104 landing.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a device of bacterial culture medium partial shipment, includes support (101) and revolving rack (102), its characterized in that still includes:

the tube clamps (103) are circumferentially arranged on the rotating frame (102), each tube clamp (103) clamps one test tube (104), and a solid culture medium is arranged in each test tube (104);

the vertical frame (105) is arranged on the support (101), and the vertical frame (105) is provided with a vertical sliding groove (106);

the lead screw (107) is vertically arranged, the lead screw (107) is in bearing connection with the vertical frame (105), the lead screw (107) is connected with a first power device (108), and the first power device (108) is electrically connected with a controller;

the screw sleeve (109) is in threaded connection with the screw rod (107), and the outer wall of the screw sleeve (109) is in sliding connection with the sliding groove (106);

the annular hot air pipe (110) is connected with the nut sleeve (109), the annular hot air pipe (110) is communicated with a hot air supply device, a plurality of air nozzles (111) are arranged on the inner wall of the annular hot air pipe (110), and the inner wall of the annular hot air pipe (110) can be sleeved outside the test tube (104) on the tube clamp (103).

2. An apparatus for dispensing an inoculum medium according to claim 1, wherein the tube clamp (103) is pivotally connected to the rotating frame (102), a slide block (201) is provided at one side of the bottom end of the support (101), the slide block (201) is provided with an arc-shaped guide surface (202), the arc-shaped guide surface (202) abuts against the side wall of the bottom end of the tube clamp (103), and the slide block (201) is used for driving the bottom end of the tube clamp (103) to deflect axially towards the rotating frame (102).

3. The device for subpackaging the strain culture medium as claimed in claim 2, wherein a maintaining frame (301) is further arranged between the pipe clamp (103) and the rotating frame (102), the maintaining frame (301) is fixedly connected with the pipe clamp (103), a rotating shaft (302) is arranged on the side wall of the maintaining frame (301), the rotating shaft (302) is pivotally connected with the rotating frame (102), a coil spring (303) is arranged on the rotating frame (102), one end of the coil spring (303) is connected with the rotating frame (102), and the other end of the coil spring (303) is connected with the rotating shaft (302).

4. The apparatus for separately containing an inoculum culture medium according to claim 3, wherein a fixture block (401) is provided on the rotating shaft (302), a stopper (402) is provided on the rotating rack (102), the stopper (402) can be clamped with the fixture block (401), and the fixture block (401) and the stopper (402) are used for limiting the rotation angle of the rotating shaft (302).

5. The apparatus for separately packaging a bacterial culture medium according to claim 1, wherein the nut sleeve (109) is provided with a temperature sensor (5), the temperature sensor (5) is electrically connected to the controller, the controller is electrically connected to the hot air supply device, the temperature sensor (5) is configured to detect a real-time temperature value of the annular hot air pipe (110), a predetermined temperature value is preset in the controller, the controller is configured to compare the real-time temperature value with the predetermined temperature value, and when the real-time temperature value is greater than the predetermined temperature value, the controller controls the hot air supply device to stop operating.

6. The apparatus for dispensing an inoculum medium of claim 1, wherein said turret (102) is further electrically connected to a second power means (6), said second power means (6) being electrically connected to said controller.

7. An apparatus for dispensing a bacterial culture medium according to claim 1, wherein the tube clamp (103) is provided with a circular platform hole (701), the test tube (104) is inserted into the circular platform hole (701), and a rubber pad (702) is arranged between the circular platform hole (701) and the test tube (104).

8. An apparatus for dispensing an inoculum medium as defined in claim 7, wherein the side walls of said circular platform aperture (701) are tapered.