CN112111400A

CN112111400A - Stem cell culture system

Info

Publication number: CN112111400A
Application number: CN202011027769.4A
Authority: CN
Inventors: 孙震颖
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-09-26
Filing date: 2020-09-26
Publication date: 2020-12-22

Abstract

The invention discloses a stem cell culture system, which comprises an installation box, a pretreatment mechanism, a cell dispersion mechanism, an isolation cleaning mechanism, a convenient test tube taking and placing mechanism, a temperature control mechanism and a cell centrifugal separation mechanism, the bottom end of the installation box is fixedly connected with an installation bottom plate, one side surface of the outer part of the installation box is provided with a cell centrifugal separation mechanism, the cell centrifugal separation mechanism comprises a centrifuge body, one side of the outer side of the installation box is provided with a centrifuge body, and one side of the centrifuge body is hinged with a sealing cover, the cell centrifugal separation mechanism enables the lifting outer pipe to rise from the top of the lifting circular pipe through the reset spring, enables the plane of the top end of the connecting turnover plate to be horizontal, and ensures the level of the top end plane of the fixing ring and the placing pipe, the positions of the placing tube and the test tube on the convenient test tube taking and placing mechanism correspond to each other, so that the test tube is more easily placed in the placing tube, thereby the cell dispersing device and the stem cell centrifugal separation mechanism are more convenient to be matched for use.

Description

Stem cell culture system

Technical Field

The invention relates to the technical field of stem cell induced differentiation, in particular to a stem cell culture system.

Background

Stem cells are cells with unlimited or immortal self-renewal capacity, which can generate at least one type of highly differentiated daughter cells, most biologists and medical scientists currently consider that stem cells are cells from embryos, fetuses or adults with unlimited self-renewal and proliferation and differentiation capacity under certain conditions, can generate daughter cells with completely the same phenotype and genotype as the stem cells, can also generate specialized cells which form body tissues and organs, and can also differentiate into progenitor cells, and during the process of inducing and differentiating the stem cells, corresponding stem cells need to be extracted from adult tissue samples;

however, the technical content of the adult tissue sample cleaning and shearing process in the stem cell extraction stage is low, but the operation is complicated, and a large amount of time is needed for operators, so that the time for inducing differentiation of the whole cells is prolonged, the efficiency for inducing differentiation of the stem cells is reduced, the operators cannot use more time for the operation with high technical content, and the quality of finished products obtained by inducing differentiation of the stem cells is indirectly reduced;

the patent belongs to an improved invention, and is a further improvement on the basis of the original technical scheme of the applicant, and the inventor finds that the test tube placing rack in the existing centrifuge usually adopts an inclined design mode, so that the test tube and the test tube rack are not easily aligned in the process of transferring the test tube through a convenient test tube taking and placing mechanism, and the transfer efficiency of the test tube is reduced.

Disclosure of Invention

In order to achieve the purpose, the invention provides the following technical scheme:

a stem cell culture system comprises an installation box, a pretreatment mechanism, a cell dispersion mechanism, an isolation cleaning mechanism, a convenient test tube taking and placing mechanism, a temperature control mechanism and a cell centrifugal separation mechanism, wherein the bottom end of the installation box is fixedly connected with an installation bottom plate, a lofting opening is formed in the middle of the top end of the installation box, and a sampling opening is formed in the bottom of the front side of the installation box; a cell centrifugal separation mechanism is arranged on one side surface outside the installation box and comprises a centrifuge main body; a sealing cover is hinged to one side of the installation box, the centrifuge body is placed on one side of the external side of the installation box, a driving disc is fixedly connected to the top end of an output shaft of a motor at the bottom of the internal side of the centrifuge body, transmission columns are uniformly and fixedly connected to the circumferential direction of the edge of the top end of the driving disc, and a collecting cylinder is placed at the top of the driving disc; the middle part of the bottom end of the inner side of the collecting cylinder is fixedly connected with a connecting chassis, the middle part of the top end of the connecting chassis is fixedly connected with a connecting round box, the middle part of the top end of the connecting round box is fixedly connected with a fixed round pipe, the circumferential direction of the outer side of the fixed round pipe is uniformly and fixedly connected with a guide chute, the inner side of the guide chute is fixedly connected with a lifting round pipe corresponding to the inner position of the fixed round pipe, the middle part of the inner side of the lifting round pipe is fixedly connected with a limiting disc, the bottom end of the limiting disc is fixedly connected with; the middle part of the top end of the lifting circular tube is rotatably connected with a rotating button, the middle part of the bottom end of the rotating button is fixedly connected with a rotating vertical rod, the bottom end of the rotating vertical rod is fixedly connected with a limiting head, two sides of the top end of the limiting head are fixedly connected with limiting convex points, the middle part of the connecting circular box is provided with a limiting special-shaped hole with the same shape as the cross section of the limiting head, the middle parts of the top end of the connecting circular box corresponding to the two sides of the limiting special-shaped hole are symmetrically provided with limiting circular holes, the positions of the limiting head and the limiting special-shaped hole correspond to each other, and the; the lifting pipe is characterized in that a guide inner pipe is fixedly connected to the top end portion of the top end of the lifting circular pipe, a lifting outer pipe is slidably connected to the top portion of the outer side of the guide inner pipe, a reset spring is fixedly connected to the bottom end portion of the inner side of the guide inner pipe, the top end of the reset spring is tightly attached to the top portion of the inner side of the lifting outer pipe, a support frame is uniformly and fixedly connected to the top end portion of the outer side of the guide inner pipe in the circumferential direction, a connecting turning plate is uniformly hinged to the top portion of the outer side of the lifting outer pipe in the circumferential direction, the middle of the connecting turning plate is rotatably connected with the; the middle part of the inner side of the sealing cover is fixedly connected with an installation circular plate, the middle part of one side of the installation circular plate is rotatably connected with a pressing ejector head, and the pressing ejector head corresponds to the cavity at the top of the lifting outer tube in position.

Has the advantages that: 1. the lifting outer tube is lifted from the top of the lifting round tube through the reset spring, the connecting turning plate is driven to rotate along the direction of a rotating shaft at the top of the support frame in the lifting process of the lifting round tube, and the plane at the top end of the connecting turning plate is restored to be horizontal when the lifting round tube is lifted to the highest point, so that the horizontal of the fixing ring and the plane at the top end of the placing tube is ensured, the central axis of the placing tube is kept vertical, the placing tube corresponds to the test tube on the convenient taking and placing mechanism of the test tube, the test tube is more easily placed in the placing tube, and the cell dispersing device and the stem cell centrifugal separation mechanism are more convenient to use in a matched manner; the lifting round tube rotates when being pressed downwards by the rotating button, so that the rotating button drives the rotating rod and the limiting head to rotate together, the limiting convex point is withdrawn from the inside of the limiting round hole, the limiting head can be withdrawn from the connecting round box through the limiting special-shaped hole, the lifting round tube is further upwards lifted from the inside of the fixed round tube along the guide sliding groove under the action of the ejection spring and the limiting disc, the placing tube and the test tube inside the placing tube are ejected out of the inside of the centrifuge main body, the centrifuged test tube is more convenient to take out, and the process of taking out the test tube inside the stem cell centrifugal separation mechanism is optimized; through the driving-disc, the cooperation between transmission post and the collection jar, at the inside independent cavity that forms of centrifuge main part, can collect the liquid of leaking, thereby the effectual inside damage that causes stem cell centrifugation mechanism of centrifuge main part that has avoided the direct leakage of liquid, it is more convenient to make the clearance of centrifuge main part simultaneously, the inside clean degree of centrifuge main part has been ensured, the test tube has been avoided at centrifuge main part internal mode secondary pollution, the quality of the stem cell of drawing has been ensured.

2. The flushing pump is started to guide flushing liquid in the flushing liquid tank into the square pipe, the flushing liquid is sprayed into the flushing tank through the liquid spraying head, so that the tissue sample is flushed, the flushing liquid flows into the flushing liquid tank again through the return hose at the bottom of the flushing box, the flushing liquid flowing into the flushing liquid tank again can be reused after being filtered by the cell filter screen, the flushing efficiency of the tissue sample is effectively improved, meanwhile, the using efficiency of the flushing liquid is improved by recycling the flushing liquid, and the waste of the flushing liquid is avoided; through the meshing transmission of the two driving gears and the transmission gear, the transverse cutting roller and the longitudinal cutting roller synchronously rotate in the same direction, so that the tissue sample is cut into strips through the transverse cutting roller, then the strip-shaped tissue sample is cut into small blocks through the longitudinal cutting roller, and finally the cut small blocks of tissue sample fall into the material guide hopper to complete the cleaning and cutting of the tissue sample; through the cooperation of each mechanical structure, realized that the automatic convenient washing of tissue sample is handled with the stripping and slicing, the effectual step of having avoided these technical content to hang down and complex operation needs the manual operation of operating personnel to go on, the effectual efficiency that improves the tissue preliminary treatment has alleviateed operating personnel's burden, makes operating personnel can put into the step of high technical content with more time in, and then improved the finished product quality of stem cell induced differentiation.

3. The collagenase solution is sprayed to the outer sides of the cutting plate, the transverse cutting roller and the longitudinal cutting roller through the transverse liquid distribution pipe, so that the outer sides of the cutting plate, the transverse cutting roller and the longitudinal cutting roller are lubricated, and meanwhile, tissues remained on the outer sides of the cutting plate, the transverse cutting roller and the longitudinal cutting roller can be washed to the material guide hopper, so that the waste of tissue sample raw materials is reduced; the utility model discloses a pretreatment mechanism, including the guide fill, the guide fill is held in the guide fill, the horizontal cutting roller simultaneously, the cooperation of vertical cutting roller and clearance comb, use the horizontal cutting roller to carry out the slitting to the tissue sample earlier, because banding tissue sample individual still has great weight, thereby its card of effectual having avoided is jammed in the clearance between the horizontal cutting sword, through the cooperation between vertical cutting roller and the clearance comb, when carrying out the dicing to the tissue sample through vertical cutting roller, clear up the cutting sword clearance department of vertical cutting roller through the clearance comb, thereby make the tissue sample can be smooth drop to in the guide fill, thereby the use of pretreatment mechanism has been optimized.

4. The dispersion tank is driven to intermittently rotate by the stepping motor, the mixed solution is stirred by the stirring plate while the dispersion tank rotates, so that the dispersion of tissue cells is accelerated, the supporting seat and all components mounted on the supporting seat are driven to descend by contracting the vertical telescopic rod, the inner cavity of the dispersion tank is communicated with the inner cavity of the liquid guide conical pipe by the liquid through square holes, the mixed solution in the dispersion tank can be discharged from the bottom of the liquid guide conical pipe and then discharged into a collection test pipe at the bottom through the positioning round holes, the complicated operation of manually transferring the mixed solution by an operator is avoided, the dispersion efficiency of the tissue to the cells is improved, the transfer process of the mixed solution is optimized, and the speed of the mixed solution from the dispersion tank to the collection test pipe is quicker; inside tissue piece and collagenase solution direction dispersion jar that will drop through the design of guide calotte, protect step motor and dispersion jar junction simultaneously to ensure cell dispersion mechanism's normal use, keep apart step motor through the heat-proof box, thereby the effectual heat transfer that has prevented step motor work production leads to dispersion jar interior solution temperature too high in leading to the dispersion jar in, influence the active phenomenon of cell in the tissue, further optimized histocyte's dispersion process.

5. The rack is drawn out from the inside of the installation box together with the components placed on the rack by drawing the sliding bottom plate along the guide box, the lower installation round box and the upper installation round box are lifted from the rack by using the lifting plates on two sides of the top end of the upper installation round box buckled by a thumb and a middle finger, the drive pressure rod is pressed down by a forefinger, the lower installation round box is made to slide downwards under the drive of the press-down drive pressure rod by overcoming the tension of a fixed spring, the lower installation round box moves downwards, the lower installation round box is driven by the matching of a drive guide pillar and an arc guide groove to rotate, so that the lower clamping arc plate and the upper clamping arc plate are separated from each other, the restraint on the collection test tube is cancelled, the collection test tube falls into the placing groove of the centrifugal machine, the transfer speed of the collection test tube is effectively improved, and the transfer of the collection test tube is more convenient.

6. The circulating fan drives the air in the heating box to flow, the electric heating pipe heats the air flowing through the heating box, the heated air enters the air guide tubule through the hot air guide pipe to heat the inside of the installation box, so that the temperature suitable for survival of tissue cells can be continuously kept in the operation process of the equipment in the installation box, and the survival rate of the cells is improved; meanwhile, the air flow in the air guide thin tube is conveyed to the interior of the air exhaust flat tube through the air supply pipe and is blown into the flushing groove through the air blowing head, the air blown to the flushing groove is sucked and guided into the heating box again through the air return pipe and the air suction box, the drying of the flushed tissue sample is accelerated, the internal circulation of the air flow is realized, and meanwhile, the repeated filtration of the filter plate is carried out in the circulation process, so that the impurities in the circulating air flow can be effectively reduced, the cleanliness of the circulating air flow in the installation box is ensured, the pollution of the impurities in the outside air in the extraction process of the stem cells is avoided, and the product quality of the stem cell induced differentiation product is effectively improved; simultaneously through the crisscross design of filter and electrothermal tube for the electrothermal tube heats the filter when heating air, makes the heating region of air in the heating cabinet bigger, thereby has improved the heating efficiency of air, thereby the effectual utilization ratio that improves heat energy has reduced the utilization ratio of the energy.

7. The cleaning liquid in the cleaning liquid tank is conveyed to the interior of the liquid conveying pipe through the cleaning pump, the cleaning liquid is sprayed onto the cutting plate, the transverse cutting roller and the longitudinal cutting roller through the upper spraying pipe and the lower spraying pipe to clean the cutting plate, the transverse cutting roller and the longitudinal cutting roller, and the cleaning liquid is sprayed into the interior of the dispersion tank through the annular spraying pipe to clean the dispersion tank, so that the cleanliness of the interior of the installation tank is ensured, and meanwhile, the cleaning process of each component in the equipment is optimized; simultaneously through the design of spacing curb plate and isolation swash plate, cut apart the installation box inside, make the clearance waste liquid at spacing curb plate top enter into the flat box of collection from the flowing back notch in, and the inside clearance waste liquid of dispersion jar is from the drain taper pipe discharge back of dispersion jar bottom, can get into to collect inside the end box through the flowing back round hole, and then collect through leading-in collection box inside of drain standpipe, carry out the layering through the clearance waste liquid with upper and lower floor and collect, the effectual washing liquid that contains large granule impurity of having avoided the upper strata enters into the inside secondary pollution that causes of dispersion jar of lower floor, the cleaning efficiency of installation box inner assembly has been improved.

Drawings

FIG. 1 is a schematic diagram of the system architecture of the present invention;

FIG. 2 is a schematic structural diagram of a stem cell centrifugal separation mechanism according to the present invention;

FIG. 3 is a schematic view of the internal structure of the collecting cylinder of the present invention;

FIG. 4 is a schematic view of the spacing head mounting structure of the present invention;

FIG. 5 is a schematic perspective view of the present invention;

FIG. 6 is a schematic view of the internal structure of the mounting box of the present invention;

FIG. 7 is a schematic view of a cutting board mounting arrangement of the present invention;

FIG. 8 is a schematic view of the mechanism position structure of the present invention;

FIG. 9 is a schematic view of the pipe connection of the present invention;

FIG. 10 is a schematic diagram of the pretreatment mechanism of the present invention;

FIG. 11 is a schematic view of a cell strainer mounting structure of the present invention;

FIG. 12 is a schematic view of the cell dispersing mechanism of the present invention;

FIG. 13 is a schematic view of the isolation cleaning mechanism of the present invention;

FIG. 14 is a schematic view of the convenient pick and place mechanism for test tubes of the present invention;

FIG. 15 is a schematic view of the drive ram mounting structure of the present invention;

fig. 16 is a schematic structural view of the temperature control mechanism of the present invention.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.

Example (b): as shown in fig. 1-4, a stem cell culture system comprises an installation box 2, a pretreatment mechanism 5, a cell dispersion mechanism 7, an isolation cleaning mechanism 8, a convenient test tube taking and placing mechanism 9, a temperature control mechanism 10 and a cell centrifugal separation mechanism 200, wherein the bottom end of the installation box 2 is fixedly connected with an installation bottom plate 1, the middle part of the top end of the installation box 2 is provided with a lofting opening 3, and the bottom of the front surface of the installation box 2 is provided with a sampling opening 4; a cell centrifugal separation mechanism 200 is arranged on one side surface of the outer part of the installation box 2, and the cell centrifugal separation mechanism 200 comprises a centrifuge main body 201;

a sealing cover 202 is hinged to one side of the outer side of the installation box 2, the centrifuge main body 201 is placed on one side of the outer side of the installation box 2, a driving disc 203 is fixedly connected to the top end of an output shaft of a motor at the bottom of the inner side of the centrifuge main body 201, transmission columns 204 are uniformly and fixedly connected to the circumferential direction of the edge portion of the top end of the driving disc 203, and a collection;

the middle part of the bottom end of the inner side of the collecting cylinder 205 is fixedly connected with a connecting chassis 206, the middle part of the top end of the connecting chassis 206 is fixedly connected with a connecting round box 207, the middle part of the top end of the connecting round box 207 is fixedly connected with a fixed round tube 208, the circumferential direction of the outer side of the fixed round tube 208 is uniformly and fixedly connected with a guide sliding groove 209, the inner side of the guide sliding groove 209 corresponding to the inner position of the fixed round tube 208 is fixedly connected with a lifting round tube 210, the middle part of the inner side of the lifting round tube 210 is fixedly connected with a limiting disc 211, the bottom end of the limiting;

the middle part of the top end of the lifting circular tube 210 is rotatably connected with a rotating button 213, the middle part of the bottom end of the rotating button 213 is fixedly connected with a rotating vertical rod 214, the bottom end of the rotating vertical rod 214 is fixedly connected with a limiting head 215, both sides of the top end of the limiting head 215 are fixedly connected with limiting convex points 216, the middle part of the connecting circular box 207 is provided with a limiting special-shaped hole 217 with the same cross section shape as the limiting head 215, the middle parts of both sides of the limiting special-shaped hole 217 corresponding to the top end of the connecting circular box 207 are symmetrically provided with limiting circular holes 218, the positions of the limiting head 215 and the limiting special-shaped;

the top end part of the top end of the lifting round tube 210 is fixedly connected with a guide inner tube 219, the top part of the outer side of the guide inner tube 219 is slidably connected with a lifting outer tube 220, the bottom end part of the inner side of the guide inner tube 219 is fixedly connected with a reset spring 221, the top end of the reset spring 221 is tightly attached to the top part of the inner side of the lifting outer tube 220, a support frame 222 is uniformly and fixedly connected to the top part of the outer side of the guide inner tube 219 in the circumferential direction, a connecting turning plate 223 is uniformly hinged to the top part of the outer side of the lifting outer tube 220 in the circumferential direction, the middle part of the connecting turning plate 223 is rotatably connected with the top part of;

the middle part of the inner side of the sealing cover 202 is fixedly connected with an installation circular plate 226, the middle part of one side of the installation circular plate 226 is rotatably connected with a pressing top 227, the pressing top 227 corresponds to the cavity position of the top of the lifting outer tube 220, the lifting outer tube 220 is lifted from the top of the lifting circular tube 210 through a return spring 221, the lifting circular tube 210 is lifted, the connecting turning plate 223 is driven to rotate along the direction of a rotating shaft at the top of the support frame 222, and when the lifting circular tube 210 is lifted to the highest point, the top end plane of the connecting turning plate 223 is restored to be horizontal, so that the top end planes of the fixing ring 224 and the placing tube 225 are ensured to be horizontal, the central axis of the placing tube 225 is kept vertical, the placing tube 225 corresponds to the position of a test tube on the convenient test;

the lifting circular tube 210 is pressed downwards by the rotating button 213 and rotates at the same time, so that the rotating button 213 drives the rotating vertical rod 214 and the limiting head 215 to rotate together, after the limiting salient point 216 exits from the inside of the limiting circular hole 218, the limiting head 215 can exit from the connecting circular box 207 through the limiting special-shaped hole 217, the lifting circular tube 210 is further lifted upwards from the inside of the fixed circular tube 208 along the guide sliding groove 209 under the action of the ejection spring 212 and the limiting disc 211, and the placing tube 225 and the test tube inside the placing tube are ejected out of the centrifuge main body 201; through the matching among the driving disc 203, the transmission column 204 and the collection cylinder 205, an independent cavity is formed in the centrifuge main body 201, and leaked liquid can be collected, so that the damage of the stem cell centrifugal separation mechanism 200 caused by the direct leakage of the liquid in the centrifuge main body 201 is effectively avoided, meanwhile, the cleaning of the centrifuge main body 201 is more convenient, the cleaning degree of the interior of the centrifuge main body 201 is ensured, and the secondary pollution of a test tube in the centrifuge main body 201 is avoided;

as shown in fig. 4-16, the bottom end of the installation box 2 is fixedly connected with an installation bottom plate 1, the middle part of the top end of the installation box 2 is provided with a lofting opening 3, the bottom of the front side of the installation box 2 is provided with a sampling opening 4, and the top of the inner side of the installation box 2 is provided with a pretreatment mechanism 5; a mounting transverse plate 501 is mounted on one side of the top of the inner side of the mounting box 2 through screws, a flushing liquid box 502 is fixedly mounted at the top end of the mounting transverse plate 501, a flushing pump 503 is connected to the position, corresponding to one side of the outer side of the mounting box 2, of the bottom of one side face of the flushing liquid box 502 through a pipeline, the input end of the flushing pump 503 is electrically connected with the output end of an external power supply, and a flushing square pipe 504 is fixedly mounted at the position, corresponding to the top end of the inner side;

horizontal telescopic rods 505 are symmetrically and fixedly arranged on the inner side of the installation box 2 corresponding to the two sides of the top of the flushing liquid box 502, flushing boxes 506 are fixedly arranged at the tail ends of the horizontal telescopic rods 505 on the two sides in the installation box 2, a flushing groove 507 is formed in the top end of each flushing box 506, collecting holes 508 are uniformly formed in the edge portion of the bottom end of each flushing groove 507, the middle portions of the bottom ends of the two flushing boxes 506 are respectively connected to the two sides of the flushing liquid box 502 through backflow hoses 509, and a cell filter screen 510 is fixedly arranged in the middle;

a cutting plate 511 is fixedly arranged at the position, corresponding to the top of the flushing box 506, of the inner side of the installation box 2, a cutting motor 512 is fixedly arranged at one side, corresponding to the cutting plate 511, of the back of the installation box 2, the input end of the cutting motor 512 is electrically connected with the output end of an external power supply, a transverse cutting roller 513 is fixedly connected at the position, corresponding to one side of the cutting plate 511, of one end of an output shaft of the cutting motor 512, a longitudinal cutting roller 514 is rotatably connected at the position, corresponding to the bottom of the transverse cutting roller 513, of the inner side of the installation box 2, and a cleaning comb 515 is fixedly connected;

one ends of the outer sides of the transverse cutting roller 513 and the longitudinal cutting roller 514, which are close to the cutting motor 512, are respectively sleeved with a driving gear 516, and a transmission gear 517 is rotatably connected at a position, corresponding to the position between the two driving gears 516, of the inner side of the installation box 2;

a solution tank 518 is fixedly installed in the middle of the back of the installation box 2, the top end of the solution tank 518 is connected with a solution pump 519 through a pipeline, the input end of the solution pump 519 is electrically connected with the output end of an external power supply, a transverse liquid distribution pipe 520 is fixedly connected to the position, corresponding to the top end of the cutting plate 511, of the top of the solution pump 519, liquid spray heads are fixedly connected to the positions, corresponding to the top of the washing groove 507, of the inner side of the washing square pipe 504, point to the bottom of the washing groove 507, the two washing boxes 506 are tightly attached to each other, sealing rubber strips are arranged on the side faces of the two washing; the cutting edges on the longitudinal cutting roller 514 and the comb teeth on the cleaning comb 515 are distributed in a staggered manner, the width size of the comb teeth is smaller than the distance size between the two cutting edges, and the transmission gears 517 are respectively meshed with the two driving gears 516;

one side of the liquid distribution transverse pipe 520 close to the transverse cutting roller 513 is uniformly provided with solution spray heads, outlets of the solution spray heads point to the inclined plane at the top of the cutting plate 511, the washing pump 503 is started to guide washing liquid in the washing liquid tank 502 into the washing square pipe 504, the washing liquid is sprayed into the washing groove 507 through the liquid spray heads, so that the tissue sample is washed, the washing liquid flows into the washing liquid tank 502 again through the return hose 509 at the bottom of the washing box 506, and the washing liquid flowing into the washing liquid tank 502 again can be reused after being filtered through the cell filter screen 510;

through the meshing transmission of the two driving gears 516 and the transmission gear 517, the transverse cutting roller 513 and the longitudinal cutting roller 514 synchronously rotate in the same direction, so that the tissue sample is cut into strips through the transverse cutting roller 513, then the strip-shaped tissue sample is cut into small blocks through the longitudinal cutting roller 514, and finally the cut small blocks of tissue sample fall into the material guide hopper 6 to complete the cleaning and cutting of the tissue sample;

the collagenase solution is sprayed to the outer sides of the cutting plate 511, the transverse cutting roller 513 and the longitudinal cutting roller 514 through the liquid distribution transverse pipe 520, so that the outer sides of the cutting plate 511, the transverse cutting roller 513 and the longitudinal cutting roller 514 are lubricated, and meanwhile, tissues remained on the outer sides of the cutting plate 511, the transverse cutting roller 513 and the longitudinal cutting roller 514 can be flushed to the material guide hopper 6; meanwhile, the transverse cutting roller 513, the longitudinal cutting roller 514 and the cleaning comb 515 are matched, the transverse cutting roller 513 is firstly used for cutting the tissue sample, and the strip-shaped tissue sample still has larger weight, so that the tissue sample is effectively prevented from being jammed in a gap between transverse cutting edges, and through the matching between the longitudinal cutting roller 514 and the cleaning comb 515, the tissue sample is diced through the longitudinal cutting roller 514, and meanwhile, the gap between the cutting edges of the longitudinal cutting roller 514 is cleaned through the cleaning comb 515, so that the tissue sample can smoothly fall into the material guide hopper 6;

a material guide hopper 6 is welded at the position, corresponding to the bottom of the cutting plate 511, on the inner side of the installation box 2, and a cell dispersing mechanism 7 is arranged at the position, corresponding to the bottom of the material guide hopper 6, on the inner side of the installation box 2; a supporting transverse plate 701 is fixedly connected to the inner side of the installation box 2 corresponding to the bottom of the material guide hopper 6, a vertical telescopic rod 702 is fixedly connected to the middle of the top end of the supporting transverse plate 701, a supporting seat 703 is fixedly connected to the middle of the top end of the vertical telescopic rod 702, a heat insulation box 704 is fixedly installed at the edge of the top end of the supporting seat 703, a stepping motor 705 is fixedly installed at the position of the top end of the supporting seat 703 corresponding to the inner side of the heat insulation box 704, a dispersion tank 706 is fixedly installed at the top of an output shaft of the stepping motor 705 through bolts, a material guide dome 707 is sleeved at;

a sealing spring 709 is uniformly and fixedly connected to the circumferential direction of the edge part at the bottom end of the dispersing tank 706, a lifting ring 710 is fixedly connected to the bottom end of the sealing spring 709, a limiting flat block 716 is uniformly and fixedly connected to the position, corresponding to the sealing spring 709, of the top end of the lifting ring 710, a liquid guiding taper pipe 711 is uniformly and fixedly connected to the position, corresponding to the limiting flat block 716, of the top end of the lifting ring 710, and a liquid through square hole 712 is uniformly formed in the circumferential direction of a straight cylinder section at the top of the liquid guiding taper pipe; the bottom end of the lifting ring 710 is uniformly and fixedly connected with a driving spring 713 in the circumferential direction, the bottom end of the driving spring 713 is fixedly connected with a supporting round block 714, the top end of the supporting transverse plate 701 corresponding to the bottom end of the liquid guiding conical pipe 711 is uniformly provided with positioning round holes 715, and the diameter of each positioning round hole 715 is larger than the outer diameter of the liquid guiding conical pipe 711;

lead liquid taper pipe 711 and run through dispersion tank 706 bottom, and lead liquid taper pipe 711 top plane and the inboard bottom plane of dispersion tank 706 flush, it rotates to drive dispersion tank 706 through step motor 705, stir mixed solution through stirring board 708 in dispersion tank 706 pivoted, thereby organize the dispersion of cell with higher speed, it descends to drive supporting seat 703 and each subassembly of installing on it through shrink vertical telescopic link 702, through leading to liquid square hole 712 with dispersion tank 706 inner chamber and the switch-on of leading liquid taper pipe 711 inner chamber, make the inside mixed liquid of dispersion tank 706 can be followed and led liquid taper pipe 711 bottom discharge, and then discharge in the collection test tube 914 of bottom through location round hole 715.

The dropped tissue blocks and collagenase solution are guided into the dispersion tank 706 through the design of the material guide dome 707, and meanwhile, the joint of the stepping motor 705 and the dispersion tank 706 is protected, so that the normal use of the cell dispersion mechanism 7 is ensured, the stepping motor 705 is isolated through the heat insulation box 704, and therefore the phenomenon that the temperature of the solution in the dispersion tank 706 is too high and the activity of cells in the tissue is influenced due to the fact that heat generated by the work of the stepping motor 705 is transferred into the dispersion tank 706 is effectively prevented, and the dispersion process of the tissue cells is further optimized.

An isolation cleaning mechanism 8 is arranged at the position corresponding to the position between the material guide hopper 6 and the dispersion tank 706 on the inner side of the installation box 2; limiting side plates 801 are symmetrically and fixedly connected to positions, corresponding to positions between the material guide hopper 6 and the dispersing tank 706, on the inner side of the mounting box 2, the top ends of the two limiting side plates 801 are movably connected with isolating inclined plates 802 through matching of T-shaped blocks and T-shaped grooves, two sides of the bottom end of each limiting side plate 801 are symmetrically and fixedly connected with accommodating mounting boxes 803, one end of the inner side of each accommodating mounting box 803 is fixedly connected with a clamping spring 804, one end of each clamping spring 804 is connected to the bottom end of each isolating inclined plate 802 through a connecting plate, the edge parts of the bottom ends of the two isolating inclined plates 802 are symmetrically and fixedly connected with electromagnets 805, the input ends of the electromagnets 805 are electrically connected with the input end of an;

a cleaning liquid box 807 is placed at one side outside the installation box 2, a cleaning pump 808 is fixedly connected at the top end of the cleaning liquid box 807 through a pipeline, the input end of the cleaning pump 808 is electrically connected with the output end of an external power supply, a liquid feeding pipe 809 is fixedly connected at the top end of the cleaning pump 808, an upper spraying pipe 810 is fixedly connected at the position, corresponding to the top of the transverse cutting roller 513, of one side of the liquid feeding pipe 809, a lower spraying pipe 811 is fixedly connected at the position, corresponding to one side of the longitudinal cutting roller 514, of the top end of one side of the liquid feeding pipe 809, and an annular spraying pipe 812 is connected at the position;

the two sides of the installation box 2 are embedded with the collection flat boxes 813 corresponding to the bottom end edge parts of the limiting side plates 801, the top end of the installation bottom plate 1 is fixedly connected with the collection bottom box 814 corresponding to the inner side of the installation box 2, liquid discharge round holes 815 are uniformly formed in the top ends of the convex surfaces on the two sides and the concave surface in the middle of the top of the collection bottom box 814, the bottom end edge part of the outer side of the installation box 2 is fixedly connected with the collection box 817, the collection flat boxes 813 and the collection bottom box 814 are communicated with the collection box 817 through liquid guide vertical pipes 816, and the magnetic poles of the opposite ends of;

the top space of the limiting side plate 801 is communicated with the inner space of the collecting flat box 813 through a liquid drainage notch 806, cleaning liquid in the cleaning liquid box 807 is conveyed to the inside of a liquid conveying pipe 809 through a cleaning pump 808, the cleaning liquid is sprayed onto the cutting plate 511, the transverse cutting roller 513 and the longitudinal cutting roller 514 through an upper spray pipe 810 and a lower spray pipe 811 to clean the cutting plate, the transverse cutting roller 513 and the longitudinal cutting roller 514, and the cleaning liquid is sprayed into the dispersing tank 706 through an annular spray pipe 812 to clean the dispersing tank;

the interior of the installation box 2 is divided by the design of the limiting side plate 801 and the isolation inclined plate 802, so that the cleaning waste liquid at the top of the limiting side plate 801 enters the flat collecting box 813 from the liquid drainage notch 806, and after the cleaning waste liquid in the dispersion tank 706 is discharged from the liquid guide conical pipe 711 at the bottom of the dispersion tank 706, the cleaning waste liquid enters the bottom collecting box 814 through the liquid guide circular hole 815, is further guided into the collecting box 817 through the liquid guide vertical pipe 816 for collection, and is collected in a layered manner by the cleaning waste liquid in the upper layer and the cleaning waste liquid in the lower layer;

the middle part of the top end of the collecting bottom box 814 is provided with a test tube convenient taking and placing mechanism 9; the two sides of the top of the concave surface of the collecting bottom box 814 are fixedly connected with guide boxes 901, a sliding bottom plate 913 is movably connected between the two guide boxes 901 through the matching of a sliding chute and a sliding block, the middle of the top of the sliding bottom plate 913 is fixedly connected with a placing frame 902, the top of the placing frame 902 is movably connected with a lower mounting round box 903 through a small bump and a groove, a lower clamping arc plate 904 is uniformly and fixedly connected with the bottom of the outer side of the lower mounting round box 903 in the circumferential direction, a driving guide post 905 is uniformly and fixedly connected with the outer side of the top of the lower mounting round box 903 in the circumferential direction, a fixed spring 906 is fixedly connected with the middle of the inner side of the lower mounting round box 903 in the bottom, the top of the fixed spring 906 corresponds to the outer side of the lower;

the middle part of the bottom end of the inner side of the lower mounting round box 903 is fixedly connected with a driving pressure rod 909, the top part of the outer side of the upper mounting round box 907 is uniformly and fixedly connected with an upper clamping arc plate 910 in the circumferential direction, the two sides of the top end of the upper mounting round box 907 are symmetrically and fixedly connected with lifting plates 911, the inner arc surfaces of the lower clamping arc plate 904 and the upper clamping arc plate 910 are both bonded with a silica gel gasket 912, a collection test tube 914 is clamped between the lower clamping arc plate 904 and the upper clamping arc plate 910, and the length of the sliding blocks 913 on the two sides of the sliding bottom plate is one tenth;

the outer side of the lower mounting round box 903 is tightly attached to the inner wall of the upper mounting round box 907, the lower mounting round box 903 is in sliding connection with the upper mounting round box 907, the driving guide pillar 905 is always located in the arc-shaped guide groove 908, and the driving guide pillar 905 can slide along the inner wall of the arc-shaped guide groove 908;

the driving pressure rod 909 penetrates through the middle part of the top end of the upper mounting circular box 907, the driving pressure rod 909 is positioned above the top end plane of the upper mounting circular box 907, the lower clamping circular plate 904 is opposite to and matched with the opening direction of the upper clamping circular plate 910, the placing frame 902 and all components placed on the placing frame are drawn out of the mounting box 2 by drawing the sliding bottom plate 913 along the guide box 901, the lower mounting circular box 903 and the upper mounting circular box 907 are lifted from the placing frame 902 by using the thumb and the middle finger to buckle the lifting plates 911 on two sides of the top end of the upper mounting circular box 907, the lower mounting circular box 903 and the upper mounting circular box 907 are pushed down by the index finger to enable the lower mounting circular box 903 to slide downwards under the driving of the pushing down driving pressure rod 909 and overcome the pulling force of the fixed spring 906, and in the process that the lower mounting circular box 903 moves downwards, the lower mounting circular box 903 is driven to rotate by the matching of the driving guide post 905 and the arc-shaped, the restriction on the collection test tube 914 is further cancelled, so that the collection test tube 914 falls into a placing groove of a centrifuge, the transfer speed of the collection test tube 914 is effectively improved, and the transfer of the collection test tube 914 is more convenient;

the bottom of the back of the installation box 2 is provided with a temperature control mechanism 10; the bottom of the back of the installation box 2 is fixedly connected with a heating box 1001, the top of one side of the heating box 1001 is fixedly connected with a circulating fan 1002, the input end of the circulating fan 1002 is electrically connected with the output end of an external power supply, filter plates 1003 are uniformly and fixedly installed on the inner side of the heating box 1001, electric heating tubes 1004 are fixedly installed on the inner side of the heating box 1001 corresponding to the top of the filter plates 1003, hot air guide pipes 1005 are fixedly connected to positions corresponding to two sides inside the installation box 2 at one end of the top of the heating box 1001, wind guide thin pipes 1006 are uniformly and fixedly connected to the top ends of the hot air guide thin pipes 1006, the top ends of the wind guide thin pipes 1006 are fixedly connected to the bottom of the air supply pipes 1007, an air exhaust flat pipe 1008 is fixedly connected to the position;

an air return pipe 1010 is fixedly connected with the air inlet end of the circulating fan 1002, an air suction box 1011 is fixedly connected with one end of the air return pipe 1010 corresponding to the other side of the top end of the flushing box 506, the filter plates 1003 and the electric heating pipes 1004 are distributed in a staggered mode, and the air guide thin pipes 1006 penetrate through the limiting side plates 801 on the two sides of the installation box 2 respectively;

the outlet of the blowing head 1009 points to the bottom of the inner side of the washing groove 507, the circulating fan 1002 drives the air in the heating box 1001 to flow, the electric heating pipe 1004 heats the air flowing through the heating box 1001, the heated air enters the air guide tubule 1006 through the hot air conduit 1005 to heat the inside of the installation box 2, so that the temperature suitable for survival of tissue cells can be continuously kept in the operation process of the equipment in the installation box 2, and the survival rate of the cells is improved;

meanwhile, the air flow in the air guide tubule 1006 is conveyed to the interior of the air exhaust flat pipe 1008 through the air supply pipe 1007 and is blown into the flushing groove 507 through the air blowing head 1009, finally, under the action of the circulating fan 1002, the air blown to the flushing groove 507 is sucked and guided into the heating box 1001 again through the air return pipe 1010 and the air suction box 1011, the drying of the flushed tissue sample is accelerated, the internal circulation of the air flow is realized, meanwhile, the impurities in the circulating air flow can be effectively reduced through the repeated filtering of the filter plate 1003 in the circulating process, the cleanliness of the circulating air flow in the installation box 2 is ensured, the pollution of the impurities in the outside air in the extraction process of the stem cells is avoided, and the product quality of the stem cell induced differentiation product is effectively improved;

meanwhile, by the staggered design of the filter plates 1003 and the electric heating tubes 1004, the electric heating tubes 1004 heat the filter plates 1003 while heating air, so that the heating area of the air in the heating box 1001 is larger, the heating efficiency of the air is improved, the utilization rate of heat energy is effectively improved, and the utilization rate of energy is reduced;

the cell dispersing device comprises the following specific processing steps: s1, preparing a mixed solution: firstly, a tissue sample for extracting stem cells is placed into an installation box from a lofting opening, so that the sample is cleaned and cut into small tissues through a pretreatment mechanism, then the tissue blocks are dispersed into single cell structures through a cell dispersion mechanism, and then a mixed solution is injected into a collection test tube through an isolation cleaning mechanism; s2, stem cell separation: placing the collection test tube into a centrifuge for centrifugal treatment, layering the tissue cells and the stem cells in the collection test tube, and then drawing the stem cells out of the collection test tube through an injector; s3, primary culture: inoculating the stem cells obtained by centrifugation into a corresponding culture medium for primary culture; s4, subculturing: inoculating the stem cells obtained by primary culture to a new culture medium for culture; s5, directional induction and differentiation: adding a corresponding agent for inducing differentiation into the culture medium to make the stem cells perform directional differentiation; s6, differentiation cell identification: and detecting the cells obtained by differentiation, thereby obtaining corresponding cells.

In the application process, after a corresponding tissue sample is obtained, the tissue sample is placed into the flushing groove 507 in the installation box 2 from the lofting opening 3, the flushing pump 503 is started to introduce flushing liquid in the flushing liquid box 502 into the flushing square pipe 504, the flushing liquid is sprayed into the flushing groove 507 through the liquid spraying head, so that the tissue sample is flushed, the flushing liquid in the flushing groove 507 flows into the flushing box 506 through the collecting hole 508 at the bottom of the flushing groove 507, the flushing liquid flows into the flushing liquid box 502 again through the backflow hose 509 at the bottom of the flushing box 506, and the flushing liquid flowing into the flushing liquid box 502 again can be reused after being filtered through the cell filter screen 510;

after the washed tissue sample is dried, the horizontal telescopic rods 505 on the two sides are contracted simultaneously, so that the two washing boxes 506 move towards the two sides of the installation box 2 simultaneously, the washing grooves 507 fall downwards between the cutting plate 511 and the transverse cutting roller 513, the transverse cutting roller 513 starts to rotate along with the starting of the cutting motor 512, the transverse cutting roller 513 is driven by the transverse cutting roller 513 to rotate synchronously in the same direction through the meshing transmission of the two driving gears 516 and the transmission gear 517, so that the transverse cutting roller 513 and the longitudinal cutting roller 514 rotate synchronously, the tissue sample is cut into strips through the transverse cutting roller 513, the strip-shaped tissue sample is cut into small blocks through the longitudinal cutting roller 514, and finally the cut small blocks of tissue sample fall into the material guide hopper 6 to complete the cleaning and cutting of the tissue sample;

the solution pump 519 is started when the cutting motor 512 is started, collagenase solution in the solution tank 518 is conveyed into the liquid distribution transverse pipe 520, and the collagenase solution is sprayed to the outer sides of the cutting plate 511, the transverse cutting roller 513 and the longitudinal cutting roller 514 through the liquid distribution transverse pipe 520, so that the outer sides of the cutting plate 511, the transverse cutting roller 513 and the longitudinal cutting roller 514 are lubricated, residual tissues outside the cutting plate 511, the transverse cutting roller 513 and the longitudinal cutting roller 514 can be flushed to the material guide hopper 6, waste of tissue sample raw materials is reduced, meanwhile, the transverse cutting roller 513, the longitudinal cutting roller 514 and the cleaning comb 515 are matched, the tissue sample is cut into strips by using the transverse cutting roller 513, and the strip-shaped tissue sample individuals still have larger weight, so that the tissue sample individuals are effectively prevented from being jammed in gaps between the transverse cutting edges, and through the matching between the longitudinal cutting roller 514 and the cleaning comb 515, when the tissue sample is diced by the longitudinal cutting roller 514, the clearance between the cutting edges of the longitudinal cutting roller 514 is cleaned by the cleaning comb 515, so that the tissue sample can smoothly fall into the material guide hopper 6;

when the cutting motor 512 starts to rotate, the electromagnet 805 is started, and under the action of repulsive magnetic force between the two opposite electromagnets 805, the isolation inclined plates 802 are driven to overcome the elastic force of the clamping spring 804 and move towards the two sides of the installation box 2, so that an opening is formed between the two isolation inclined plates 802, and the tissue sample and the collagenase solution in the material guide hopper 6 can be ensured to smoothly enter the dispersion tank 706;

after the tissue sample and the collagenase solution enter the dispersion tank 706, the small-sized tissue sample is gradually dispersed into independent cells under the action of the collagenase, the dispersion tank 706 is driven by the stepping motor 705 to intermittently rotate, the mixed solution is stirred by the stirring plate 708 while the dispersion tank 706 rotates, so that the dispersion of the tissue cells is accelerated, after a period of stirring and reaction, the tissue in the dispersion tank 706 is completely dispersed into independent cells, the support base 703 and the components mounted on the support base are driven to descend by contracting the vertical telescopic rod 702, the driving spring 713 starts to be compressed after the support round block 714 is contacted with the top end of the support transverse plate 701, the elastic force of the driving spring 713 is gradually greater than that of the sealing spring 709 as the dispersion tank 706 continuously descends, so that the distance between the lifting ring 710 and the dispersion tank 706 is gradually reduced, and the liquid guide cone 711 is lifted from the bottom of the dispersion tank 706, thereby through leading to liquid square hole 712 with dispersion tank 706 inner chamber and the switch-on of drain taper pipe 711 inner chamber, make the inside mixed liquid of dispersion tank 706 can discharge from drain taper pipe 711 bottom, and then discharge the collection test tube 914 of bottom through location round hole 715, the loaded down with trivial details operation of the manual mixed liquid that shifts of operating personnel has been avoided, thereby the dispersion efficiency of organizing to the cell has been improved, the transfer process of mixed liquid has been optimized, make mixed liquid from dispersion tank 706 to the speed of collecting in the test tube 914 rapider.

The dropped tissue blocks and collagenase solution are guided into the dispersion tank 706 through the design of the material guide dome 707, meanwhile, the joint of the stepping motor 705 and the dispersion tank 706 is protected, so that the normal use of the cell dispersion mechanism 7 is ensured, the stepping motor 705 is isolated through the heat insulation box 704, and the overhigh temperature of the solution in the dispersion tank 706 caused by the heat generated by the work of the stepping motor 705 being transferred into the dispersion tank 706 is effectively prevented;

after the cell mixture is introduced into the interior of the collection tube 914, the collection tube 914 is placed in a centrifuge for centrifugation, so that the tissue cells and stem cells in the mixture in the collection tube 914 are layered, thereby facilitating the extraction of the stem cells, after the sampling port 4 is opened, the rack 902 with the components placed thereon is extracted from the interior of the housing 2 by pulling the slide bottom plate 913 along the guide box 901, the lower mounting round case 903 and the upper mounting round case 907 are lifted up from the rack 902 by grasping the pulling plates 911 on both sides of the top end of the upper mounting round case 907 with the thumb and middle finger, and placed in the centrifuge through the collection tube 914, after the collection tube 914 is aligned with the placement groove in the centrifuge, the lower mounting round case 903 is slid down against the tensile force of the fixing spring 906 by depressing the index finger to drive the driving plunger 909, and during the downward movement of the lower mounting round case 903, the lower mounting round box 903 is driven to rotate by the matching of the driving guide post 905 and the arc-shaped guide groove 908, so that the lower clamping arc plate 904 and the upper clamping arc plate 910 are separated from each other, the constraint on the collection test tube 914 is cancelled, and the collection test tube 914 falls into a placing groove of the centrifuge;

in the operation process of the device, the circulating fan 1002 is started to drive the air in the heating box 1001 to flow, the air flowing through the heating box 1001 is heated by the electric heating tube 1004, the heated air enters the air guide tubule 1006 via the hot air duct 1005, the inside of the installation box 2 is heated in the process that the hot air flows through the air guide tubule 1006, so that the temperature suitable for survival of tissue cells in the operation process of the device in the installation box 2 is continuously kept, the survival rate of the cells is improved, meanwhile, the air flow in the air guide tubule 1006 is conveyed to the inside of the air exhaust flat tube 1008 via the air supply pipe 1007 and is blown into the flushing groove 507 via the air blowing head 1009, finally, under the action of the circulating fan 1002, the air blown to the flushing groove 507 is sucked and guided into the heating box 1001 again via the air return pipe 1010 and the air suction box 1011, and when the drying of the flushed tissue sample is accelerated, the internal circulation of the air flow is realized, and meanwhile, impurities in the circulating air flow can be effectively reduced through repeated filtration of the filter plate 1003 in the circulation process, so that the cleanliness of the circulating air flow in the installation box 2 is ensured, the pollution of impurities in the outside air in the stem cell extraction process is avoided, and the product quality of stem cell induced differentiation products is effectively improved; meanwhile, the filter plates 1003 are heated by the electric heating tube 1004 at the same time of heating air through the staggered design of the filter plates 1003 and the electric heating tube 1004;

after the equipment is used, the interior of the installation box 2 needs to be cleaned, cleaning liquid in the cleaning liquid box 807 is conveyed to the interior of a liquid conveying pipe 809 through a cleaning pump 808, the cleaning liquid is sprayed onto the cutting plate 511, the transverse cutting roller 513 and the longitudinal cutting roller 514 through an upper spray pipe 810 and a lower spray pipe 811 to clean the cleaning liquid, and the cleaning liquid is sprayed into the interior of the dispersion tank 706 through an annular spray pipe 812 to clean the dispersion tank;

meanwhile, through the design of the limiting side plate 801 and the isolating inclined plate 802, the interior of the installation box 2 is divided, so that the cleaning waste liquid at the top of the limiting side plate 801 enters the collecting flat box 813 from the liquid drainage notch 806, and after the cleaning waste liquid in the dispersion tank 706 is discharged from the liquid guide conical pipe 711 at the bottom of the dispersion tank 706, the cleaning waste liquid can enter the collecting bottom box 814 through the liquid drainage round hole 815, and then is guided into the collecting box 817 through the liquid guide vertical pipe 816 for collection, and the cleaning waste liquid in the upper layer and the lower layer is collected in a layered manner, so that the secondary pollution caused by the fact that the cleaning liquid containing large granular impurities in the upper layer enters the dispersion tank 706 in the lower layer is effectively avoided, and the cleaning efficiency of components in the installation box 2 is improved;

after the test tube is taken out by the convenient test tube taking and placing mechanism 9 in the cell dispersing device 100, the test tube needs to be transferred to the stem cell centrifugal separation mechanism 200 for centrifugal separation so as to extract stem cells, after the sealing cover 202 is opened, the pressing top 227 is lifted from the cavity at the top of the lifting outer tube 220 so as to cancel the limit of the corresponding lifting outer tube 220, the lifting outer tube 220 is lifted from the top of the lifting circular tube 210 under the action of the reset spring 221, in the lifting process of the lifting circular tube 210, the connecting turning plate 223 is driven to rotate along the rotating shaft direction at the top of the supporting frame 222, and when the lifting circular tube 210 is lifted to the highest point, the top plane of the connecting turning plate 223 is restored to be horizontal, so that the levels of the top planes of the fixing ring 224 and the placing tube 225 are ensured, the central axis of the placing tube 225 is kept vertical, and the positions of the placing tube 225 and the test tube, so that the test tube can be more easily placed inside the placing tube 225, and the cell dispersing device 100 and the stem cell centrifugal separation mechanism 200 can be more conveniently used in a matching manner;

in the process of taking out the test tube after centrifuging the outside, in order to make the test tube easier to take out from the inside of the centrifuge main body 201, the test tube can be jacked up from the inside of the centrifuge main body 201, the lifting circular tube 210 is pressed down by the rotating button 213 and rotates at the same time, so that the rotating button 213 drives the rotating rod 214 and the limiting head 215 to rotate together, after the limiting convex point 216 exits from the inside of the limiting circular hole 218, the limiting head 215 can exit from the connecting circular box 207 through the limiting special-shaped hole 217, further the lifting circular tube 210 is lifted upwards from the inside of the fixed circular tube 208 along the guide sliding groove 209 under the action of the ejection spring 212 and the limiting disc 211, further the placing tube 225 and the test tube inside the placing tube 225 are ejected out from the inside of the centrifuge main body 201, the centrifuged test tube is more convenient to take out;

in the process of stem cell centrifugation mechanism 200 operation, if the test tube breaks and can lead to liquid to leak centrifuge main part 201, through driving-disc 203, cooperation between transmission column 204 and the collection jar 205, an independent cavity is formed inside centrifuge main part 201, can collect the liquid of leaking, thereby effectually avoided the direct inside damage that causes stem cell centrifugation mechanism 200 of centrifuge main part 201 that leaks of liquid, it is more convenient to make the clearance of centrifuge main part 201 simultaneously, the inside clean degree of centrifuge main part 201 has been ensured, the test tube has been avoided at centrifuge main part 201 inside mode secondary pollution, the quality of the stem cell of drawing has been ensured.

Claims

1. A stem cell culture system comprises an installation box (2), a pretreatment mechanism (5), a cell dispersion mechanism (7), an isolation cleaning mechanism (8), a convenient test tube taking and placing mechanism (9), a temperature control mechanism (10) and a cell centrifugal separation mechanism (200), and is characterized in that the bottom end of the installation box (2) is fixedly connected with an installation bottom plate (1), a lofting opening (3) is formed in the middle of the top end of the installation box (2), and a sampling opening (4) is formed in the bottom of the front side of the installation box (2);

a cell centrifugal separation mechanism (200) is arranged on one side surface of the outer part of the mounting box (2), and the cell centrifugal separation mechanism (200) comprises a centrifuge main body (201);

a sealing cover (202) is hinged to one side of the outer side of the mounting box (2) where the centrifuge main body (201) is placed, a driving disc (203) is fixedly connected to the top end of an output shaft of a motor at the bottom of the inner side of the centrifuge main body (201), transmission columns (204) are uniformly and fixedly connected to the circumferential direction of the top end of the driving disc (203), and a collecting cylinder (205) is placed at the top of the driving disc (203);

the middle part of the bottom end of the inner side of the collecting cylinder (205) is fixedly connected with a connecting chassis (206), the middle part of the top end of the connecting chassis (206) is fixedly connected with a connecting round box (207), the middle part of the top end of the connecting round box (207) is fixedly connected with a fixed round pipe (208), the outer side of the fixed round pipe (208) is uniformly and fixedly connected with a guide sliding chute (209) in the circumferential direction, the inner side of the guide sliding chute (209) is fixedly connected with a lifting round pipe (210) corresponding to the inner position of the fixed round pipe (208), the middle part of the inner side of the lifting round pipe (210) is fixedly connected with a limiting disc (211), the bottom end of the limiting disc (211) is fixedly connected with an ejection spring (212;

the middle part of the top end of the lifting circular tube (210) is rotatably connected with a rotating button (213), the middle part of the bottom end of the rotating button (213) is fixedly connected with a rotating vertical rod (214), the bottom end of the rotating vertical rod (214) is fixedly connected with a limiting head (215), both sides of the top end of the limiting head (215) are fixedly connected with limiting convex points (216), the middle part of the connecting circular box (207) is provided with a limiting special-shaped hole (217) with the same cross section shape as the limiting head (215), the middle parts of the top end of the connecting circular box (207) corresponding to the limiting special-shaped hole (217) are symmetrically provided with limiting circular holes (218), the positions of the limiting head (215) and the limiting special-shaped hole (217) correspond to each other, and the positions of the limiting convex points (216) and the limiting;

the edge part of the top end of the lifting round tube (210) is fixedly connected with a guide inner tube (219), the top part of the outer side of the guide inner tube (219) is connected with a lifting outer tube (220) in a sliding way, the edge part of the bottom end of the inner side of the guide inner tube (219) is fixedly connected with a return spring (221), the top end of the return spring (221) is tightly attached to the top of the inner side of the lifting outer tube (220), the edge part of the top end of the outer side of the guide inner tube (219) is evenly and fixedly connected with a support frame (222) in the circumferential direction, a connecting turning plate (223) is uniformly hinged on the top of the outer side of the lifting outer pipe (220) in the circumferential direction, the middle part of the connecting turning plate (223) is rotationally connected with the top of the support frame (222) through a rotating shaft, a long circular groove is formed in the connecting turning plate (223) and the rotating shaft, a fixing ring (224) is fixedly connected to the tail end of the connecting turning plate (223), and a placing pipe (225) is fixedly connected to the bottom end of the fixing ring (224);

the middle part of the inner side of the sealing cover (202) is fixedly connected with an installation circular plate (226), the middle part of one side of the installation circular plate (226) is rotatably connected with a pressing top head (227), and the pressing top head (227) corresponds to the top cavity of the lifting outer pipe (220).

2. The stem cell culture system according to claim 1, wherein a mounting bottom plate (1) is fixedly connected to the bottom end of the mounting box (2), a lofting opening (3) is formed in the middle of the top end of the mounting box (2), a sampling opening (4) is formed in the bottom of the front surface of the mounting box (2), and a pretreatment mechanism (5) is arranged at the top of the inner side of the mounting box (2);

the pretreatment mechanism (5) comprises a mounting transverse plate (501), a flushing liquid tank (502), a flushing pump (503), a flushing square pipe (504), a horizontal telescopic rod (505), a flushing box (506), a flushing groove (507), a collecting hole (508), a backflow hose (509), a cell filter screen (510), a cutting plate (511), a cutting motor (512), a transverse cutting roller (513), a longitudinal cutting roller (514), a cleaning comb (515), a driving gear (516), a transmission gear (517), a solution tank (518), a solution pump (519) and a transverse liquid distribution pipe (520);

a mounting transverse plate (501) is mounted on one side of the top of the inner side of the mounting box (2) through a screw, a flushing liquid box (502) is fixedly mounted at the top end of the mounting transverse plate (501), a flushing pump (503) is connected to the position, corresponding to one side of the outer portion of the mounting box (2), of the bottom of one side face of the flushing liquid box (502) through a pipeline, the input end of the flushing pump (503) is electrically connected with the output end of an external power supply, and a flushing square pipe (504) is fixedly mounted at the position, corresponding to the top of the inner side of the mounting box (2);

horizontal telescopic rods (505) are symmetrically and fixedly installed on the inner side of the installation box (2) corresponding to the two sides of the top of the flushing liquid box (502), flushing boxes (506) are fixedly installed at the tail ends of the horizontal telescopic rods (505) on the two sides in the installation box (2), a flushing tank (507) is arranged at the top end of the flushing box (506), collecting holes (508) are uniformly formed in the edge portion of the bottom end of the flushing tank (507), the middle portions of the bottom ends of the two flushing boxes (506) are connected to the two sides of the flushing liquid box (502) through backflow hoses (509), and a cell filter screen (510) is fixedly installed in the middle portion of the inner side of the flushing liquid box;

a cutting plate (511) is fixedly mounted on the inner side of the installation box (2) corresponding to the top of the flushing box (506), a cutting motor (512) is fixedly mounted on one side of the back of the installation box (2) corresponding to the cutting plate (511), the input end of the cutting motor (512) is electrically connected with the output end of an external power supply, a transverse cutting roller (513) is fixedly connected at one end of an output shaft of the cutting motor (512) corresponding to one side of the cutting plate (511), a longitudinal cutting roller (514) is rotatably connected at the inner side of the installation box (2) corresponding to the bottom of the transverse cutting roller (513), and a cleaning comb (515) is fixedly connected at one side of the longitudinal cutting roller (514) in the installation box (2);

one ends of the outer sides of the transverse cutting roller (513) and the longitudinal cutting roller (514) close to the cutting motor (512) are sleeved with driving gears (516), and transmission gears (517) are rotatably connected to the positions, corresponding to the positions between the two driving gears (516), of the inner side of the installation box (2);

install bin (2) back middle part fixed mounting has solution tank (518), there is solution pump (519) solution tank (518) top through the pipe connection, the input of solution pump (519) and external power source's output electric connection, solution pump (519) top corresponds cutting board (511) top position department fixedly connected with cloth liquid and violently manages (520).

3. The stem cell culture system according to claim 2, wherein a liquid spraying head is fixedly connected to the inner side of the washing square tube (504) corresponding to the top of the washing groove (507), the liquid spraying head points to the bottom of the washing groove (507), two washing boxes (506) are tightly attached to each other, sealing rubber strips are arranged on the side surfaces of the two washing boxes, and the bottom surface of the washing groove (507) is of an arc-shaped structure with a high middle and two low sides;

the cutting edges on the longitudinal cutting roller (514) and the comb teeth on the cleaning comb (515) are distributed in a staggered manner, the width size of the comb teeth is smaller than the distance size between the two cutting edges, and the transmission gears (517) are respectively meshed with the two driving gears (516);

one side of the liquid distribution transverse pipe (520) close to the transverse cutting roller (513) is uniformly provided with solution spray heads, and outlets of the solution spray heads point to the top inclined plane of the cutting plate (511).

4. The stem cell culture system according to claim 2, wherein a material guide hopper (6) is welded on the inner side of the installation box (2) corresponding to the bottom of the cutting plate (511), and a cell dispersing mechanism (7) is arranged on the inner side of the installation box (2) corresponding to the bottom of the material guide hopper (6);

the cell dispersing mechanism (7) comprises a supporting transverse plate (701), a vertical telescopic rod (702), a supporting seat (703), a heat insulation box (704), a stepping motor (705), a dispersing tank (706), a material guiding dome (707), a stirring plate (708), a sealing spring (709), a lifting ring (710), a liquid guiding conical tube (711), a liquid passing square hole (712), a driving spring (713), a supporting round block (714), a positioning round hole (715) and a limiting flat block (716);

a supporting transverse plate (701) is fixedly connected at the position of the inner side of the mounting box (2) corresponding to the bottom of the material guide hopper (6), the middle part of the top end of the supporting transverse plate (701) is fixedly connected with a vertical telescopic rod (702), a supporting seat (703) is fixedly connected with the middle part of the top end of the vertical telescopic rod (702), a heat insulation box (704) is fixedly arranged at the edge part of the top end of the supporting seat (703), a stepping motor (705) is fixedly arranged at the top end of the supporting seat (703) corresponding to the inner side of the heat insulation box (704), the input end of the stepping motor (705) is electrically connected with the output end of an external power supply, the top of the output shaft of the stepping motor (705) is fixedly provided with a dispersion tank (706) through a bolt, a material guide dome (707) is sleeved in the middle of the inner side of the dispersion tank (706), and stirring plates (708) are uniformly and fixedly arranged in the circumferential direction of the inner side of the dispersion tank (706);

sealing springs (709) are uniformly and fixedly connected to the circumferential direction of the edge portion of the bottom end of the dispersion tank (706), the bottom end of each sealing spring (709) is fixedly connected with a lifting ring (710), a position, corresponding to the sealing spring (709), of the top end of each lifting ring (710) is uniformly and fixedly connected with a limiting flat block (716), a position, corresponding to the limiting flat block (716), of the top end of each lifting ring (710) is uniformly and fixedly connected with a liquid guide conical pipe (711), and liquid through square holes (712) are uniformly formed in the circumferential direction of a straight barrel section at the top of the liquid guide conical pipe (711);

the bottom end of the lifting ring (710) is uniformly and fixedly connected with a driving spring (713) in the circumferential direction, the bottom end of the driving spring (713) is fixedly connected with a supporting round block (714), the top end of the supporting transverse plate (701) corresponding to the bottom end of the liquid guiding taper pipe (711) is uniformly provided with positioning round holes (715), and the diameter of each positioning round hole (715) is larger than the outer diameter of the liquid guiding taper pipe (711);

the liquid guiding taper pipe (711) penetrates through the bottom of the dispersion tank (706), and the top end plane of the liquid guiding taper pipe (711) is flush with the bottom end plane of the inner side of the dispersion tank (706).

5. The stem cell culture system according to claim 4, wherein an isolation cleaning mechanism (8) is arranged inside the installation box (2) at a position corresponding to the position between the material guide hopper (6) and the dispersion tank (706);

the isolation cleaning mechanism (8) comprises a limiting side plate (801), an isolation inclined plate (802), a containing installation box (803), a clamping spring (804), an electromagnet (805), a liquid drainage notch (806), a cleaning liquid box (807), a cleaning pump (808), a liquid delivery pipe (809), an upper spray pipe (810), a lower spray pipe (811), an annular spray pipe (812), a flat collection box (813), a bottom collection box (814), a liquid drainage round hole (815), a liquid guide vertical pipe (816) and a collection box (817);

limiting side plates (801) are symmetrically and fixedly connected to the inner side of the installation box (2) corresponding to the position between the material guide hopper (6) and the dispersion tank (706), the top ends of the two limiting side plates (801) are movably connected with an isolation inclined plate (802) through the matching of a T-shaped block and a T-shaped groove, the two sides of the bottom end of the limit side plate (801) are symmetrically and fixedly connected with containing and installing boxes (803), a clamping spring (804) is fixedly connected at one end of the inner side of the containing and mounting box (803), one end of each clamping spring (804) is connected to the bottom ends of the isolating inclined plates (802) through a connecting plate, electromagnets (805) are symmetrically and fixedly connected to the edge parts of the bottom ends of the two isolating inclined plates (802), the input end of the electromagnet (805) is electrically connected with the input end of an external power supply, and liquid drainage notches (806) are uniformly formed in the top end of the limiting side plate (801) close to the edge of the inner wall of the installation box (2);

a cleaning liquid box (807) is placed on one side of the outer portion of the installation box (2), a cleaning pump (808) is fixedly connected to the top end of the cleaning liquid box (807) through a pipeline, the input end of the cleaning pump (808) is electrically connected with the output end of an external power supply, a liquid feeding pipe (809) is fixedly connected to the top end of the cleaning pump (808), an upper spraying pipe (810) is fixedly connected to one side of the liquid feeding pipe (809) corresponding to the top of the transverse cutting roller (513), a lower spraying pipe (811) is fixedly connected to one side of the liquid feeding pipe (809) corresponding to one side of the longitudinal cutting roller (514), and an annular spraying pipe (812) is connected to one side of the liquid feeding pipe (809) corresponding to the top of the dispersion tank (706);

install in installing bin (2) both sides correspond spacing curb plate (801) bottom limit portion position department embedding and collect flat box (813), installing floor (1) top corresponds installing bin (2) inboard position department fixedly connected with and collects end box (814), collect end box (814) top both sides convex surface and middle part concave surface top and have all evenly seted up flowing back round hole (815), installing bin (2) outside bottom limit portion fixedly connected with collects box (817), collect flat box (813) and collect end box (814) and all pass through drain standpipe (816) and collection box (817) switch-on.

6. The stem cell culture system of claim 5, wherein the magnetic poles at the opposite ends of the electromagnets (805) at the bottoms of the two isolated inclined plates (802) are the same;

the top space of the limit side plate (801) is communicated with the inner space of the flat collecting box (813) through a liquid drainage notch (806).

7. The stem cell culture system according to claim 5, wherein the collection bottom box (814) is provided with a test tube pick-and-place mechanism (9) at the middle of the top end;

the convenient test tube taking and placing mechanism (9) comprises a guide box (901), a placing rack (902), a lower mounting round box (903), a lower clamping arc plate (904), a driving guide post (905), a fixing spring (906), an upper mounting round box (907), an arc-shaped guide groove (908), a driving press rod (909), an upper clamping arc plate (910), a lifting plate (911), a silica gel gasket (912), a sliding bottom plate (913) and a collection test tube (914);

the collecting bottom box (814) is characterized in that guide boxes (901) are fixedly connected to two sides of the top of a concave surface at the top, a sliding bottom plate (913) is movably connected between the two guide boxes (901) through a sliding chute and a sliding block in a matched manner, the middle of the top of the sliding bottom plate (913) is fixedly connected with a placing frame (902), the top of the placing frame (902) is movably connected with a lower mounting round box (903) through a small bump and a groove, the bottom of the outer side of the lower mounting round box (903) is uniformly and fixedly connected with a lower clamping arc plate (904) in the circumferential direction, the top of the outer side of the lower mounting round box (903) is uniformly and fixedly connected with a driving guide post (905) in the circumferential direction, the middle of the inner side of the lower mounting round box (903) is fixedly connected with a fixing spring (906), the top of the fixing, arc-shaped guide grooves (908) are uniformly formed in the circumferential direction of the inner side of the upper mounting round box (907);

the utility model discloses a test tube collection test tube, including lower installation circle box (903), install down inboard bottom middle part fixedly connected with drive depression bar (909) of circle box (903), go up the even fixedly connected with in the circumferencial direction of installation circle box (907) outside top and press from both sides tight arc board (910), go up installation circle box (907) top bilateral symmetry fixedly connected with and carry out arm-tie (911), press from both sides tight arc board (904) down and press from both sides tight arc board (910) intrados and all bond silica gel gasket (912), the centre gripping has collection test tube (914) down between tight arc board (904) of clamp and the tight arc board (910) of last clamp.

8. The stem cell culture system of claim 7, wherein the length of the sliding blocks on both sides of the sliding base plate (913) is one tenth of the length of the guide box (901);

the outer side of the lower installation round box (903) is tightly attached to the inner wall of the upper installation round box (907), the lower installation round box (903) is in sliding connection with the upper installation round box (907), the driving guide pillar (905) is always located in the arc-shaped guide groove (908), and the driving guide pillar (905) can slide along the inner wall of the arc-shaped guide groove (908);

the driving pressure rod (909) penetrates through the middle of the top end of the upper mounting round box (907), the driving pressure rod (909) is positioned on the top end plane of the upper mounting round box (907), and the lower clamping arc plate (904) and the upper clamping arc plate (910) are opposite in opening direction and matched with each other.

9. A stem cell culture system according to claim 6, wherein the bottom of the back of the installation box (2) is provided with a temperature control mechanism (10);

the temperature control mechanism (10) comprises a heating box (1001), a circulating fan (1002), a filter plate (1003), an electric heating pipe (1004), a hot air guide pipe (1005), an air guide thin pipe (1006), an air supply pipe (1007), an air exhaust flat pipe (1008), an air blowing head (1009), an air return pipe (1010) and an air suction box (1011);

the utility model discloses a solar energy water heater, including install bin (2), heating cabinet (1001), circulating fan (1002), circulating fan's (1002) input and external power source's output electric connection, the even fixed mounting of heating cabinet (1001) inboard has filter (1003), the inboard of heating cabinet (1001) corresponds filter (1003) top position department fixed mounting has electrothermal tube (1004), the inside both sides position department fixed connection of install bin (2) is located to heating cabinet (1001) top one end, the even fixed connection in hot-blast pipe (1005) top has wind-guiding tubule (1006), wind-guiding tubule (1006) top fixed connection is to blast pipe (1007) bottom, blast pipe (1007) end correspondence washes box (506) top one side position department fixed connection has flat pipe (1008) of airing exhaust, a blowing head (1009) is uniformly and fixedly connected at the position of one side of the air exhaust flat pipe (1008) corresponding to the edge part of the top end of the flushing tank (507);

air inlet end fixedly connected with return air pipe (1010) of circulating fan (1002), return air pipe (1010) one end corresponds and washes box (506) top opposite side position department fixedly connected with air suction box (1011).

10. The stem cell culture system of claim 9, wherein the filter plates (1003) and the electrothermal tubes (1004) are distributed in a staggered manner, and the air guide tubules (1006) respectively penetrate through the limiting side plates (801) at two sides of the installation box (2);

the outlet of the blowing head (1009) points to the bottom of the inner side of the washing groove (507).