CN115477556A - Method for preparing polypeptide plant nutrient stimulin by degrading meat and bone meal with biological enzyme method - Google Patents
Method for preparing polypeptide plant nutrient stimulin by degrading meat and bone meal with biological enzyme method Download PDFInfo
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- 235000019735 Meat-and-bone meal Nutrition 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 34
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 30
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 29
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 29
- NCYVXEGFNDZQCU-UHFFFAOYSA-N nikethamide Chemical compound CCN(CC)C(=O)C1=CC=CN=C1 NCYVXEGFNDZQCU-UHFFFAOYSA-N 0.000 title claims abstract description 23
- 230000000593 degrading effect Effects 0.000 title claims abstract description 12
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 9
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 9
- 235000015097 nutrients Nutrition 0.000 title claims description 20
- 239000007788 liquid Substances 0.000 claims abstract description 84
- 238000010438 heat treatment Methods 0.000 claims abstract description 49
- 239000012752 auxiliary agent Substances 0.000 claims abstract description 37
- 239000000843 powder Substances 0.000 claims abstract description 31
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 15
- 239000008103 glucose Substances 0.000 claims abstract description 15
- 239000012263 liquid product Substances 0.000 claims abstract description 13
- 229920002261 Corn starch Polymers 0.000 claims abstract description 9
- 239000008120 corn starch Substances 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 238000007670 refining Methods 0.000 claims abstract description 7
- 230000001863 plant nutrition Effects 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims description 37
- 238000005086 pumping Methods 0.000 claims description 30
- 210000000988 bone and bone Anatomy 0.000 claims description 25
- 235000013372 meat Nutrition 0.000 claims description 21
- 241000196324 Embryophyta Species 0.000 claims description 19
- 102000004157 Hydrolases Human genes 0.000 claims description 18
- 108090000604 Hydrolases Proteins 0.000 claims description 18
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 18
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 18
- 238000001816 cooling Methods 0.000 claims description 13
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 12
- 238000003860 storage Methods 0.000 claims description 12
- 229940088598 enzyme Drugs 0.000 claims description 8
- 241000194108 Bacillus licheniformis Species 0.000 claims description 7
- 108010011619 6-Phytase Proteins 0.000 claims description 6
- 108091005508 Acid proteases Proteins 0.000 claims description 6
- 241000193417 Brevibacillus laterosporus Species 0.000 claims description 6
- 102000004882 Lipase Human genes 0.000 claims description 6
- 108090001060 Lipase Proteins 0.000 claims description 6
- 239000004367 Lipase Substances 0.000 claims description 6
- 108010070551 Meat Proteins Proteins 0.000 claims description 6
- 108010020346 Polyglutamic Acid Proteins 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 235000019421 lipase Nutrition 0.000 claims description 6
- 229940085127 phytase Drugs 0.000 claims description 6
- 229920002643 polyglutamic acid Polymers 0.000 claims description 6
- 235000010333 potassium nitrate Nutrition 0.000 claims description 6
- 239000004323 potassium nitrate Substances 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 239000000413 hydrolysate Substances 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 230000002255 enzymatic effect Effects 0.000 claims description 3
- 210000001835 viscera Anatomy 0.000 claims description 2
- 238000006911 enzymatic reaction Methods 0.000 claims 3
- 239000004519 grease Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 13
- 239000000047 product Substances 0.000 abstract description 6
- 238000005265 energy consumption Methods 0.000 abstract description 4
- 235000021049 nutrient content Nutrition 0.000 abstract description 4
- 238000002360 preparation method Methods 0.000 abstract description 4
- 150000001413 amino acids Chemical class 0.000 description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 6
- 230000007062 hydrolysis Effects 0.000 description 6
- 238000006460 hydrolysis reaction Methods 0.000 description 6
- 239000003337 fertilizer Substances 0.000 description 5
- 235000018102 proteins Nutrition 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000009278 visceral effect Effects 0.000 description 5
- 244000144972 livestock Species 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
- 108010038807 Oligopeptides Proteins 0.000 description 3
- 102000015636 Oligopeptides Human genes 0.000 description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 3
- 238000005903 acid hydrolysis reaction Methods 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 229910052742 iron Inorganic materials 0.000 description 3
- 239000011777 magnesium Substances 0.000 description 3
- 229910052749 magnesium Inorganic materials 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 239000003895 organic fertilizer Substances 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- 239000002002 slurry Substances 0.000 description 3
- 239000011701 zinc Substances 0.000 description 3
- 229910052725 zinc Inorganic materials 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 239000002374 bone meal Substances 0.000 description 2
- 229940036811 bone meal Drugs 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000005238 degreasing Methods 0.000 description 2
- 230000006353 environmental stress Effects 0.000 description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 239000000021 stimulant Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000011344 liquid material Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/70—Controlling the treatment in response to process parameters
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention discloses a method for preparing polypeptide plant nutrition stimulin by degrading meat and bone meal with a biological enzyme method, which comprises the pretreatment steps of crushing and dissolving the meat and bone meal, adding corn starch and glucose, heating, adding an auxiliary agent A, removing oil and adjusting the pH value, the steps of primary enzymolysis, secondary enzymolysis and mixing enzymolysis liquid for two times, and the refining step of concentrating a liquid product into powder. The preparation method provided by the invention has the advantages of simple production process conditions, short production period, controllable production process, low energy consumption and high product nutrient content.
Description
Technical Field
The invention relates to the technical field of organic fertilizer production, and in particular relates to a method for preparing polypeptide plant nutrient stimulin from meat and bone meal.
Background
The meat and bone meal is a product prepared by taking livestock bodies, residual broken meat, bones, internal organs and the like which are not suitable for eating in livestock and poultry slaughter houses as raw materials through metering, mincing, melting, degreasing, pressing, cooling, drying and crushing, and is mainly used for directly or indirectly preparing fertilizers at present.
Because the plant can not directly absorb protein, the meat and bone powder must be further decomposed into amino acid or short peptide to be absorbed and utilized by the plant, so the direct application of the meat and bone powder to the fertilizer needs secondary conversion, and the fertilizer efficiency is slow.
Some manufacturers can decompose meat, bone meal or animal tissues into amino acids for recycling, and common hydrolysis methods comprise an acid hydrolysis method, an alkali hydrolysis method and a high-temperature high-pressure hydrolysis method, wherein the acid hydrolysis methods are most widely applied, but the methods have the characteristics of strong acid, strong alkali, high temperature and the like, have high requirements on equipment, site selection and operators, and produce amino acid products with low additional value (the market price is more than 500-600 yuan/ton), thereby influencing the further development of the industry.
For example, in the method for producing bio-organic fertilizer by livestock and poultry died of diseases disclosed in the Chinese patent CN 103396181B, the organic fertilizer is prepared by adding strains for fermentation, and the production period is too long and the efficiency is low. The method for producing amino acid fertilizer by utilizing waste water and meat and bone meal subjected to innocent treatment of livestock and poultry, which is disclosed by the Chinese patent CN 104003766B, adopts acidolysis and enzymatic catalytic hydrolysis methods, and hydrochloric acid is strong acid, so that the process requirement is high. Therefore, a new preparation method is needed.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a method for preparing polypeptide phytotrophy stimulin by degrading meat and bone meal with a biological enzyme method, so as to solve the problems in the background technology.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows.
A method for preparing polypeptide plant nutrition stimulin by degrading meat and bone meal with a biological enzyme method specifically comprises the following steps:
A. pretreatment:
putting the meat and bone meal into a pulverizer, pulverizing until the meat and bone meal can pass through a 60-mesh sieve; adding 2/3 of water into a first-stage enzymolysis tank, adding the crushed meat and bone meal while stirring until the Baume degree is 25, and adding a proper amount of corn starch and glucose; starting a heating device, heating to 80 ℃, and stirring for 2 hours; starting a cooling device to cool to normal temperature, adding an auxiliary agent A which is one-tenth of the liquid material amount in the primary enzymolysis tank, stirring for 2.5 hours, standing for 1 hour, then performing oil-liquid separation, and pumping away the oil; stirring and heating to 60 ℃ again, and adding potassium hydroxide to adjust the pH value to 7-9;
B. enzymolysis:
B1. stirring the feed liquid in the primary enzymolysis tank while performing primary enzymolysis, adding an auxiliary agent B with five mass percent of meat and bone meal, starting a heating device, heating to 40 +/-5 ℃, and performing constant-temperature enzymolysis for 36 hours to obtain primary enzymolysis liquid; filtering part of the first-stage enzymolysis liquid, and pumping a filtrate into a first-stage enzymolysis liquid storage tank;
B2. adding citric acid into the residual enzymolysis liquid for secondary enzymolysis to adjust the pH value to 5-6; adding an auxiliary agent C with the mass percent of meat and bone meal, starting a heating device, heating to 50 +/-5 ℃, carrying out enzymolysis for 36 hours at constant temperature, cooling to room temperature to obtain a second-stage enzymolysis liquid, and pumping the second-stage enzymolysis liquid into a second-stage enzymolysis liquid storage tank;
B3. mixing the primary enzymolysis liquid and the secondary enzymolysis liquid according to the needs of crops to obtain a liquid product of the polypeptide plant nutrient stimulin;
C. refining:
b, pumping the liquid product of the polypeptide plant nutrient stimulin prepared in the step B into a concentration tank, stirring and heating to 55 ℃, opening a vacuum system, and concentrating for 3 hours under the condition of keeping negative pressure at-0.04 MPa; closing the vacuum system, and opening a tank top pressure release valve to recover the normal pressure state in the tank; pumping the feed liquid into a spray dryer through a slurry pump to prepare powder.
The technical scheme is further optimized, wherein in the step A, the corn starch accounts for 0.1-0.2% of the mass of the meat and bone meal, and the glucose accounts for 0.05-0.15% of the mass of the meat and bone meal.
According to a further optimized technical scheme, the auxiliary agent A comprises 1-3 parts of bacillus licheniformis trophosome strain and 0.5-1.5 parts of bacillus laterosporus trophosome strain.
According to a further optimized technical scheme, the auxiliary agent B comprises 4-6 parts of meat protein hydrolase, 1-3 parts of bone protein hydrolase, 1-3 parts of visceral hydrolase, 0.5-1.5 parts of lipase and 0.2-0.4 part of polyglutamic acid powder.
According to a further optimized technical scheme, the auxiliary agent C comprises 1-3 parts of acid protease, 0.5-1.5 parts of phytase, 0.5-1.5 parts of glucose and 1.5-2.5 parts of potassium nitrate.
Due to the adoption of the technical scheme, the technical progress of the invention is as follows.
The method for preparing the polypeptide plant nutrition stimulin by degrading the meat and bone meal by the biological enzyme method overcomes the defects of complex process conditions, difficult process control, poor functionality, high energy consumption and more pollutants of the traditional hydrolysis technology of the traditional amino acid hydrolysis technology, realizes the controllable hydrolysis of the meat and bone meal under the mild condition, ensures that the hydrolysate contains rich amino acid, oligopeptides with specific molecular weight and necessary nutrient elements such as calcium, potassium, magnesium, zinc, iron and the like of crops, can directly provide nutrition for the crops, and can stimulate the growth potential of the crops and improve the capability of resisting environmental stress. The preparation method provided by the invention has the advantages of simple production process conditions, short production period, controllable production process, low energy consumption and high product nutrient content.
Detailed Description
A method for preparing polypeptide plant nutrition stimulin by degrading meat and bone meal with a biological enzyme method specifically comprises the following steps:
A. pretreatment:
putting the meat and bone meal into a pulverizer, pulverizing until the meat and bone meal can pass through a 60-mesh sieve; adding 2/3 of water into a first-stage enzymolysis tank, adding the crushed meat and bone powder while stirring until the Baume degree is 25, and adding corn starch accounting for 0.15% of the mass of the meat and bone powder and glucose accounting for 0.1% of the mass of the meat and bone powder; starting a heating device, heating to 80 ℃, and stirring for 2 hours; and opening a cooling device to cool to normal temperature, and adding an auxiliary agent A which is one ten thousandth of the amount of the feed liquid in the first-stage enzymolysis tank, wherein the auxiliary agent A comprises 1-3 parts of bacillus licheniformis vegetative body strain and 0.5-1.5 parts of bacillus laterosporus vegetative body strain. Stirring for 2.5h, standing for 1h, performing oil-liquid separation, and pumping out oil; stirring and heating to 60 ℃ again, and adding potassium hydroxide to adjust the pH value to 7-9.
The materials coming out of the chemical preparation tank on the meat and bone meal production line can be directly crushed by the crusher and then added into the first-stage enzymolysis tank for pretreatment, and finished meat and bone meal is prepared without the processes of degreasing and pressing in the later period, so that the production cost and the energy consumption are reduced.
The auxiliary agent A in the step contains two strains, and the two strains can be propagated in the meat bone meal liquid with relatively rich nutrition and mainly play the roles in 3 aspects: firstly, the two bacteria can decompose a plurality of glycerinum and fatty acid substances in the meat and bone meal liquid, so that the interface of the oil liquid is clear, the later separation is convenient, and the oil liquid interface is prevented from being fuzzy after the stirring is static to influence the oil removal effect; secondly, the bacillus licheniformis can generate protease in the propagation process to promote the next enzymolysis process; thirdly, the two strains are high-efficiency strains for manufacturing the biological fertilizer, and the physiological activity of the final product applied to plants can be improved.
B. Enzymolysis:
B1. and (3) stirring the feed liquid in the primary enzymolysis tank while performing primary enzymolysis, and adding an auxiliary agent B with five mass percent of meat and bone meal into the primary enzymolysis tank, wherein the auxiliary agent B comprises 4-6 parts of meat protein hydrolase, 1-3 parts of bone protein hydrolase, 1-3 parts of visceral hydrolase, 0.5-1.5 parts of lipase and 0.2-0.4 part of polyglutamic acid powder. Starting a heating device, heating to 40 +/-5 ℃, and carrying out constant-temperature enzymolysis for 36 hours to obtain a primary enzymolysis liquid; and filtering part of the first-stage enzymolysis liquid, and pumping the filtrate into a first-stage enzymolysis liquid storage tank.
The assistant B is a complex enzyme suitable for working under the condition of medium temperature and alkalinity, and has a decomposition rate of 70-75% for animal protein.
B2. Adding citric acid into the residual enzymolysis liquid and the enzymolysis liquid for regulating the pH value to 5-6 by secondary enzymolysis; adding auxiliary agent C with two mass percent of meat and bone powder, wherein the auxiliary agent C comprises 1-3 parts of acid protease, 0.5-1.5 parts of phytase, 0.5-1.5 parts of glucose and 1.5-2.5 parts of potassium nitrate. And (3) starting a heating device, heating to 50 +/-5 ℃, carrying out enzymolysis for 36 hours at constant temperature, cooling to room temperature to obtain a second-stage enzymolysis liquid, and pumping the second-stage enzymolysis liquid into a second-stage enzymolysis liquid storage tank.
The assistant C can decompose the protein which can not be decomposed by the assistant A.
B3. And mixing the primary enzymolysis liquid and the secondary enzymolysis liquid according to the needs of crops to obtain a liquid product of the polypeptide plant nutrient stimulin. The two parts are combined to improve the decomposition efficiency of the enzymatic hydrolysis meat and bone meal, two different enzymatic hydrolysis products are obtained, and various plant nutrition stimulin products with different functions can be obtained by compounding the two parts in different proportions.
C. Refining:
b, pumping the liquid product of the polypeptide plant nutrient stimulin prepared in the step B into a concentration tank, stirring and heating to 55 ℃, opening a vacuum system, and concentrating for 3 hours under the condition of keeping negative pressure at-0.04 MPa; closing the vacuum system, and opening a tank top pressure release valve to recover the normal pressure state in the tank; pumping the feed liquid into a spray dryer through a slurry pump to prepare powder.
The present invention will be described in further detail with reference to specific examples.
Example 1:
A. pretreatment:
putting the meat and bone meal into a grinder, grinding the meat and bone meal until the meat and bone meal can pass through a 60-mesh sieve; adding 2/3 of water into a first-stage enzymolysis tank, adding the crushed meat and bone powder while stirring until the Baume degree is 25, and adding corn starch accounting for 0.15% of the mass of the meat and bone powder and glucose accounting for 0.1% of the mass of the meat and bone powder; starting a heating device, heating to 80 ℃, and stirring for 2 hours; and (3) starting a cooling device to cool to normal temperature, and adding an auxiliary agent A which is one ten thousandth of the feed liquid in the primary enzymolysis tank, wherein the auxiliary agent A comprises 2 parts of bacillus licheniformis vegetative body strain and 1 part of bacillus laterosporus vegetative body strain. Stirring for 2.5h, standing for 1h, performing oil-liquid separation, and pumping out oil; stirring and heating to 60 deg.C again, and adding potassium hydroxide to adjust pH to 8.
B. Enzymolysis:
B1. and (2) stirring the feed liquid in the primary enzymolysis tank while performing primary enzymolysis, and adding an auxiliary agent B with five mass percent of meat and bone meal into the primary enzymolysis tank, wherein the auxiliary agent B comprises 5 parts of meat protein hydrolase, 2 parts of bone protein hydrolase, 2 parts of visceral hydrolase, 1 part of lipase and 0.3 part of polyglutamic acid powder. Starting a heating device, heating to 43 ℃, and carrying out constant-temperature enzymolysis for 36 hours to obtain primary enzymolysis liquid; and filtering half of the first-stage enzymolysis liquid, and pumping the filtrate into a first-stage enzymolysis liquid storage tank.
B2. Adding citric acid into the residual enzymolysis liquid and the enzymolysis liquid for regulating the pH value to 5.5 by secondary enzymolysis; adding an auxiliary agent C with the mass percentage of two meat and bone powder, wherein the auxiliary agent C comprises 2 parts of acid protease, 1 part of phytase, 1 part of glucose and 2 parts of potassium nitrate. And starting a heating device, heating to 53 ℃, carrying out enzymolysis for 36 hours at constant temperature, cooling to room temperature to obtain a second-stage enzymolysis liquid, and pumping the second-stage enzymolysis liquid into a second-stage enzymolysis liquid storage tank.
The assistant C can decompose the protein which can not be decomposed by the assistant A.
B3. And mixing the primary enzymolysis liquid and the secondary enzymolysis liquid according to the needs of crops to obtain a liquid product of the polypeptide plant nutrient stimulin.
C. Refining:
b, pumping the liquid product of the polypeptide plant nutrient stimulin prepared in the step B into a concentration tank, stirring and heating to 55 ℃, opening a vacuum system, and concentrating for 3 hours under the condition of keeping negative pressure at-0.04 MPa; closing the vacuum system, and opening a tank top pressure release valve to recover the normal pressure state in the tank; and pumping the feed liquid into a spray dryer through a mud pump to prepare powder.
Example 2:
A. pretreatment:
putting the meat and bone meal into a pulverizer, pulverizing until the meat and bone meal can pass through a 60-mesh sieve; adding 2/3 of water into the first-stage enzymolysis tank, adding the crushed meat and bone powder while stirring until the Baume degree is 25, and adding corn starch accounting for 0.15% of the mass of the meat and bone powder and glucose accounting for 0.1% of the mass of the meat and bone powder; starting a heating device, heating to 80 ℃, and stirring for 2 hours; and (3) starting a cooling device to cool to normal temperature, and adding an auxiliary agent A which is one ten thousandth of the amount of the feed liquid in the first-stage enzymolysis tank, wherein the auxiliary agent A comprises 1 part of bacillus licheniformis vegetative body strain and 0.5 part of bacillus laterosporus vegetative body strain. Stirring for 2.5h, standing for 1h, separating oil from liquid, and pumping away oil; stirring and heating to 60 deg.C, and adding potassium hydroxide to adjust pH to 7.
B. Enzymolysis:
B1. and (3) stirring the feed liquid in the primary enzymolysis tank while performing primary enzymolysis, and adding an auxiliary agent B with five mass percent of meat and bone meal into the primary enzymolysis tank, wherein the auxiliary agent B comprises 4 parts of meat protein hydrolase, 1 part of bone protein hydrolase, 1 part of visceral hydrolase, 0.5 part of lipase and 0.2 part of polyglutamic acid powder. Starting a heating device, heating to 40 ℃, and carrying out constant-temperature enzymolysis for 36 hours to obtain primary enzymolysis liquid; and filtering one third of the first-level enzymolysis liquid, and pumping the filtrate into a first-level enzymolysis liquid storage tank.
B2. Adding citric acid into the residual enzymolysis liquid and the enzymolysis liquid for regulating the pH value to 5 by secondary enzymolysis; adding an auxiliary agent C with two mass percent of meat and bone meal, wherein the auxiliary agent C comprises 1 part of acid protease, 0.5 part of phytase, 0.5 part of glucose and 1.5 parts of potassium nitrate. And starting a heating device, heating to 50 ℃, carrying out enzymolysis for 36 hours at constant temperature, cooling to room temperature to obtain a second-stage enzymolysis liquid, and pumping the second-stage enzymolysis liquid into a second-stage enzymolysis liquid storage tank.
The assistant C can decompose the protein which can not be decomposed by the assistant A.
B3. And mixing the primary enzymolysis liquid and the secondary enzymolysis liquid according to the needs of crops to obtain a liquid product of the polypeptide plant nutrient stimulin.
C. Refining:
b, pumping the liquid product of the polypeptide plant nutrient stimulin prepared in the step B into a concentration tank, stirring and heating to 55 ℃, opening a vacuum system, and concentrating for 3 hours under the condition of keeping negative pressure at-0.04 MPa; closing the vacuum system, and opening a tank top pressure release valve to recover the normal pressure state in the tank; pumping the feed liquid into a spray dryer through a slurry pump to prepare powder.
Example 3:
A. pretreatment:
putting the meat and bone meal into a pulverizer, pulverizing until the meat and bone meal can pass through a 60-mesh sieve; adding 2/3 of water into a first-stage enzymolysis tank, adding the crushed meat and bone powder while stirring until the Baume degree is 25, and adding corn starch accounting for 0.15% of the mass of the meat and bone powder and glucose accounting for 0.1% of the mass of the meat and bone powder; starting a heating device, heating to 80 ℃, and stirring for 2 hours; and (3) starting a cooling device to cool to normal temperature, and adding an auxiliary agent A which is one ten thousandth of the feed liquid in the primary enzymolysis tank, wherein the auxiliary agent A comprises 3 parts of bacillus licheniformis vegetative mass strain and 1.5 parts of bacillus laterosporus vegetative mass strain. Stirring for 2.5h, standing for 1h, performing oil-liquid separation, and pumping out oil; stirring again and heating to 60 deg.C, and adding potassium hydroxide to adjust pH to 9.
B. Enzymolysis:
B1. and (3) stirring the feed liquid in the primary enzymolysis tank while performing primary enzymolysis, and adding an auxiliary agent B with five mass percent of meat and bone meal into the primary enzymolysis tank, wherein the auxiliary agent B comprises 6 parts of meat protein hydrolase, 3 parts of bone protein hydrolase, 3 parts of visceral hydrolase, 1.5 parts of lipase and 0.4 part of polyglutamic acid powder. Starting a heating device, heating to 45 ℃, and carrying out constant-temperature enzymolysis for 36 hours to obtain primary enzymolysis liquid; and filtering two thirds of the first-level enzymolysis liquid, and pumping the filtrate into a first-level enzymolysis liquid storage tank.
B2. Adding citric acid into the residual enzymolysis liquid for secondary enzymolysis to adjust the pH value to 6; then adding an auxiliary agent C with two mass percent of meat and bone powder, wherein the auxiliary agent C comprises 3 parts of acid protease, 1.5 parts of phytase, 1.5 parts of glucose and 2.5 parts of potassium nitrate. And starting a heating device, heating to 55 ℃, carrying out enzymolysis for 36 hours at constant temperature, cooling to room temperature to obtain a second-stage enzymolysis liquid, and pumping the second-stage enzymolysis liquid into a second-stage enzymolysis liquid storage tank.
The assistant C can decompose the protein which can not be decomposed by the assistant A.
B3. And mixing the primary enzymolysis liquid and the secondary enzymolysis liquid according to the needs of crops to obtain a liquid product of the polypeptide plant nutrient stimulin.
C. Refining:
b, pumping the liquid product of the polypeptide plant nutrient stimulating hormone prepared in the step B into a concentration tank, stirring, heating to 55 ℃, opening a vacuum system, and concentrating for 3 hours under the condition of keeping negative pressure of-0.04 MPa; closing the vacuum system, and opening a tank top pressure release valve to recover the normal pressure state in the tank; and pumping the feed liquid into a spray dryer through a mud pump to prepare powder.
The polypeptide phytotrophy stimulants prepared in examples 1 to 3 were subjected to the measurement of the nutrient content, and the average value was used to represent the measured nutrient content of the polypeptide phytotrophy stimulants prepared according to the present invention. The contents of the components are as follows:
table 1: polypeptide plant nutrient stimulin nutrient component
| Active ingredient | Content (a) of |
| Molecular weight, da | 180～1800 |
| Specific oligopeptide, g/L | 46.3 |
| Amino acid, g/L | 18.2 |
| Nucleic acid, g/L | 11.6 |
| Organic calcium, magnesium, zinc, iron, g/L | 5.6 |
| Water-insoluble matter, g/L | 0.002 |
As can be seen from the table, the polypeptide plant nutrient stimulin hydrolysate prepared by the invention contains abundant amino acids, oligopeptides with specific molecular weights, and nutrient elements such as calcium, potassium, magnesium, zinc, iron and the like which are necessary for crops, and not only can directly provide nutrition for the crops, but also can stimulate the growth potential of the crops and improve the capability of resisting environmental stress.
Claims (5)
1. A method for preparing polypeptide plant nutrition stimulin by degrading meat and bone meal with a biological enzyme method is characterized by comprising the following steps:
A. pretreatment:
putting the meat and bone meal into a pulverizer, pulverizing until the meat and bone meal can pass through a 60-mesh sieve; adding 2/3 of water into a first-stage enzymolysis tank, adding the crushed meat and bone meal while stirring until the Baume degree is 25, and adding a proper amount of corn starch and glucose; starting a heating device, heating to 80 ℃, and stirring for 2 hours; starting a cooling device to cool to normal temperature, adding an auxiliary agent A which is one ten thousandth of the amount of the feed liquid in the primary enzymolysis tank, stirring for 2.5 hours, standing for 1 hour, then carrying out oil-liquid separation, and pumping away the grease; stirring and heating to 60 ℃ again, and adding potassium hydroxide to adjust the pH value to 7-9;
B. enzymolysis:
B1. stirring the feed liquid in the primary enzymolysis tank while adding an auxiliary agent B with five mass percent of meat and bone powder, starting a heating device, heating to 40 +/-5 ℃, and carrying out constant-temperature enzymolysis for 36 hours to obtain primary enzymolysis liquid; filtering part of the first-stage enzymolysis liquid, and pumping a filtrate into a first-stage enzymolysis liquid storage tank;
B2. adding citric acid into the residual enzymolysis liquid and the enzymolysis liquid for regulating the pH value to 5-6 by secondary enzymolysis; adding an auxiliary agent C with the mass percent of meat and bone meal, starting a heating device, heating to 50 +/-5 ℃, carrying out enzymolysis for 36 hours at constant temperature, cooling to room temperature to obtain a second-stage enzymolysis liquid, and pumping the second-stage enzymolysis liquid into a second-stage enzymolysis liquid storage tank;
B3. mixing the primary enzymatic hydrolysate and the secondary enzymatic hydrolysate according to the needs of crops to obtain a liquid product of the polypeptide plant nutrient stimulin;
C. refining:
b, pumping the liquid product of the polypeptide plant nutrient stimulin prepared in the step B into a concentration tank, stirring and heating to 55 ℃, opening a vacuum system, and concentrating for 3 hours under the condition of keeping negative pressure at-0.04 MPa; closing the vacuum system, and opening a tank top pressure release valve to recover the normal pressure state in the tank; and pumping the feed liquid into a spray dryer through a mud pump to prepare powder.
2. The method for preparing polypeptide phytotrophin by degrading meat and bone meal with a bio-enzyme method according to claim 1, wherein: in the step A, the corn starch accounts for 0.1-0.2% of the mass of the meat and bone meal, and the glucose accounts for 0.05-0.15% of the mass of the meat and bone meal.
3. The method for preparing polypeptide phytotrophin by degrading meat and bone meal with a bio-enzymatic method according to claim 1, wherein: the auxiliary agent A comprises 1-3 parts of bacillus licheniformis trophosome strain and 0.5-1.5 parts of bacillus laterosporus trophosome strain.
4. The method for preparing polypeptide phytotrophin by degrading meat and bone meal with a bio-enzymatic method according to claim 1, wherein: the auxiliary agent B comprises 4-6 parts of meat protein hydrolase, 1-3 parts of bone protein hydrolase, 1-3 parts of viscera hydrolase, 0.5-1.5 parts of lipase and 0.2-0.4 part of polyglutamic acid powder.
5. The method for preparing polypeptide phytotrophin by degrading meat and bone meal with a bio-enzymatic method according to claim 1, wherein: the assistant C comprises 1-3 parts of acid protease, 0.5-1.5 parts of phytase, 0.5-1.5 parts of glucose and 1.5-2.5 parts of potassium nitrate.
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| CN1785058A (en) * | 2005-11-30 | 2006-06-14 | 天津农学院 | Nutrient liquor made from sheep bone enzymolysis polypeptide, and its prepn. method |
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| CN102115774A (en) * | 2010-11-30 | 2011-07-06 | 华东理工大学 | Method for preparing plant polypeptide by enzyme process |
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| CN108359658A (en) * | 2018-02-23 | 2018-08-03 | 河南仰韶生化工程有限公司 | Food-grade meat proteins hydrolase and salt taste essence and meat hydrolysis process for treating |
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| CN1785058A (en) * | 2005-11-30 | 2006-06-14 | 天津农学院 | Nutrient liquor made from sheep bone enzymolysis polypeptide, and its prepn. method |
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| CN102115774A (en) * | 2010-11-30 | 2011-07-06 | 华东理工大学 | Method for preparing plant polypeptide by enzyme process |
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