CN115475704A - Centrifugal tube bearing device, sample gradient separation equipment and liquid adding method of centrifugal tube - Google Patents

Abstract

The invention relates to the technical field of centrifugation, and particularly discloses a centrifugal tube bearing device, sample gradient separation equipment and a liquid adding method of a centrifugal tube. The centrifugal tube bearing device comprises a base, a first bearing frame and a power mechanism; the first bearing frame is rotatably connected with the base; the first bearing frame is used for bearing the centrifuge tube; the power mechanism is used for driving the first bearing frame to rotate relative to the base, so that the centrifuge tube arranged on the first bearing frame is in an inclined state. The centrifugal tube bearing device provided by the embodiment of the invention has higher automation degree, and higher sample separation effect and separation efficiency.

Description

Centrifugal tube bearing device, sample gradient separation equipment and liquid adding method of centrifugal tube

Technical Field

The invention relates to the technical field of centrifugation, in particular to a centrifugal tube bearing device, sample gradient separation equipment and a liquid adding method of a centrifugal tube.

Background

Before a liquid-based sample is separated, the sample which is uniformly mixed through elution needs to be transferred to a centrifugal tube filled with a gradient separation liquid, so that the sample is suspended above the gradient separation liquid as much as possible, but when the sample is added into the centrifugal tube at present, the sample can quickly sink and directly mix with the gradient separation liquid under the action of gravity, and the sample separation effect is poor and the separation efficiency is low.

Disclosure of Invention

One of the purposes of the embodiments of the present invention is to provide a centrifugal tube carrying device, which at least solves the problems of poor gradient separation effect and low efficiency of the existing liquid-based sample.

In order to achieve the above purpose, the embodiment of the present invention adopts the following technical solutions:

a centrifugal tube bearing device comprises a base, a first bearing frame and a power mechanism;

the first bearing frame is rotatably connected with the base;

the first bearing frame is used for bearing a centrifuge tube;

the power mechanism is used for driving the first bearing frame to rotate relative to the base, so that the centrifugal tube arranged on the first bearing frame is in an inclined state.

In one possible embodiment, the centrifuge tube carrier further comprises a reset mechanism provided at the base and/or the first carrier to reset the centrifuge tube in an inclined state.

In one possible embodiment, the return mechanism includes a first torsion spring disposed between the first carrier and the base;

or the resetting mechanism comprises a magnetic part, and the magnetic part is arranged on the first bearing frame and/or the base;

or, the reset mechanism comprises a first torsion spring and a magnetic piece, and the first torsion spring is arranged between the first bearing frame and the base; the magnetic part is arranged on the first bearing frame and/or the base.

In one possible embodiment, the base comprises a base, a first bracket and a second bracket; the first bracket and the second bracket are respectively arranged at two opposite ends of the base and extend upwards from the base;

the first bearing frame is arranged between the first support and the second support and is respectively connected with the first support and the second support in a rotating mode.

In a possible embodiment, the first bearing frame comprises a bearing main body, a first connecting shaft and a second connecting shaft, wherein the first connecting shaft and the second connecting shaft are arranged on two opposite sides of the bearing main body and are coaxially arranged; the first connecting shaft is rotationally connected with the first bracket; the second connecting shaft is rotatably connected with the second support.

In a possible embodiment, the bearing body is formed with a containing groove, and a notch of the containing groove faces upwards for placing the centrifugal tube;

or, bear the weight of the main part and be formed with the storage tank more than two, every the notch of storage tank is in order to be used for placing up centrifuge tube, adjacent two the storage tank interval sets up.

In a possible embodiment, the bearing body is provided with a scanning avoidance port; the scanning avoiding port is arranged on the side wall of the containing groove and communicated with the containing groove.

In a possible embodiment, the base is formed with a first limiting member, and the first limiting member is disposed on the first bracket and/or the second bracket to limit the reset position of the first loading frame.

In a possible embodiment, the base is formed with a first stopper for limiting a reset position of the first carrier; the centrifuge tube bearing device further comprises a second limiting piece, wherein the second limiting piece is arranged on the second support and/or a part of the second support, which is far away from the bottom end of the base, so as to limit the rotation amplitude of the first bearing frame to be 0-60 degrees; and the first limiting piece and the second limiting piece are respectively arranged on two opposite sides of the first bearing frame.

In a possible embodiment, the centrifuge tube carrying device further comprises a connecting rod connected with the first carrying frame, so that the power mechanism can drive the first carrying frame to rotate relative to the base;

or the centrifuge tube carrying device further comprises a connecting rod, and the connecting rod is connected with the first carrying frame so that the power mechanism can drive the first carrying frame to rotate relative to the base; the base is formed with spacing arch, spacing arch with the connecting rod is located the homonymy of base is in order to be used for right the swing of connecting rod is spacing.

In one possible embodiment, the power mechanism comprises a motor, an output shaft and a toggle assembly; the output shaft is connected with the motor, the toggle assembly is movably connected with the output shaft, and the toggle assembly toggles the first bearing frame to rotate relative to the base;

and/or, the centrifugal tube bearing device further comprises a control system, and the control system is used for controlling the power mechanism to work.

Compared with the prior art, the centrifuge tube carrying device provided by the embodiment of the invention has the advantages that the first bearing frame is rotatably connected with the base and used for bearing a centrifuge tube, and the power mechanism is used for driving the first bearing frame to rotate by taking the part connected with the base as a rotating central shaft, so that the centrifuge tube arranged on the first bearing frame is in an inclined state, on one hand, the automation degree of the centrifuge tube carrying device is improved, and the operation reliability and stability are improved, on the other hand, the centrifuge tube can be kept in the inclined state, and when a sample liquid is added into the centrifuge tube for gradient separation, the separation effect and the separation efficiency of a sample can be effectively improved.

A second objective of the embodiments of the present invention is to provide a sample gradient separation apparatus, which adopts the following technical solution:

a sample gradient separation device comprises the centrifugal tube bearing device.

In one possible embodiment, the sample gradient separation apparatus further comprises:

a transfer mechanism for holding and transferring the susceptor;

the liquid adding mechanism is used for adding the gradient separation liquid into the centrifugal tube arranged on the centrifugal tube bearing device;

and the sample adding mechanism is used for adding a sample into the centrifugal tube arranged in the centrifugal tube bearing device.

In a possible embodiment, the sample gradient separation apparatus further comprises a second carrier and a third carrier;

the second bearing frame is used for bearing the base

The third bearing frame and the second bearing frame are arranged at intervals and used for bearing the power mechanism.

In one possible embodiment, the power mechanism comprises a motor, an output shaft and a toggle assembly; the output shaft is connected with the motor, and the toggle assembly is movably connected with the output shaft;

and/or the sample gradient separation equipment further comprises a first driving mechanism, a poking arm, a first driving wheel, a second driving wheel and a driving belt connected between the first driving wheel and the second driving wheel, wherein the first driving wheel and the second driving wheel are respectively arranged at two opposite ends of the second bearing frame, the first driving mechanism is used for driving the first driving wheel or the second driving wheel to rotate, and the poking arm is connected with the driving belt; the poking arm is used for poking the base arranged on the second bearing frame to the transfer mechanism so that the power mechanism can drive the first bearing frame which is rotationally connected with the base to rotate relative to the base.

Compared with the prior art, the sample gradient separation equipment provided by the embodiment of the invention has the characteristics of high automation degree, good sample gradient separation effect, high gradient separation efficiency and the like because the centrifugal tube bearing device is included.

The third purpose of the embodiment of the invention is to provide a liquid adding method for a centrifuge tube, which adopts the following technical scheme:

a liquid adding method of a centrifuge tube comprises the following steps:

inserting a centrifuge tube into a first carrier of the sample gradient fractionating apparatus;

controlling the power mechanism to drive the first bearing frame to drive the centrifugal tube to rotate relative to the base, so that a preset angle is formed between the central shaft of the centrifugal tube and the vertical direction;

adding a gradient separation solution into the centrifuge tube;

adding a sample into the centrifuge tube;

and controlling the first bearing frame to carry the centrifuge tube to rotate and reset relative to the base until the included angle between the central shaft of the centrifuge tube and the vertical direction is 0 degree.

In one possible embodiment, before controlling the power mechanism to drive, the method further includes the step of placing the base carrying the centrifugal tube on the second carrier, and driving the base to the transfer mechanism by the transmission mechanism;

and/or controlling a liquid adding mechanism to add the gradient separation liquid into the centrifugal tube; and controlling a sample adding mechanism to add the sample into the centrifuge tube.

Compared with the prior art, the liquid adding method of the centrifuge tube provided by the embodiment of the invention uses the sample gradient separation equipment, and the centrifuge tube carrying device in the first aspect of the embodiment of the invention is included in the sample gradient separation equipment, so that the liquid adding efficiency can be effectively improved, and meanwhile, good sample separation effect and sample separation efficiency can be obtained.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

Fig. 1 is a schematic perspective view of a centrifugal tube carrying device according to a first embodiment of the present invention;

fig. 2 is a schematic front view of a centrifuge tube carrying device according to a first embodiment of the present invention;

fig. 3 is an exploded schematic view of a centrifuge tube carrying device according to an embodiment of the present invention;

fig. 4 is a simplified schematic diagram illustrating a control relationship of a centrifuge tube carrying device according to a first embodiment of the present invention;

FIG. 5 is a schematic perspective view of a sample gradient separation apparatus according to an embodiment of the present invention;

FIG. 6 is an enlarged partial view of FIG. 5 at M;

FIG. 7 is an enlarged view of a portion of FIG. 5 at N;

FIG. 8 is an enlarged partial view of FIG. 5 at P;

FIG. 9 is a schematic top view of a sample gradient separation apparatus according to an embodiment of the present invention;

FIG. 10 is an enlarged partial schematic view at Q of FIG. 9;

FIG. 11 is a simplified structural diagram of a sample gradient separation apparatus according to an embodiment of the present invention;

FIG. 12 is a simplified control diagram of a sample gradient separation apparatus according to an embodiment of the present invention;

FIG. 13 is a schematic partial longitudinal sectional view illustrating a contacting state of an output tube and a centrifuge tube in a sample gradient separation apparatus according to an embodiment of the present invention;

fig. 14 is an exploded view of a centrifuge tube carrying device according to a second embodiment of the present invention;

fig. 15 is an exploded schematic view of a centrifuge tube carrying device according to a third embodiment of the present invention.

Reference numerals:

10. a centrifuge tube carrying device;

11. a base; 111. a base; 112. a first bracket; 113. a second bracket; 114. a first limit piece; 115. a limiting bulge; 110. avoiding a space;

12. a first carrier; 121. a load bearing body; 1211. a containing groove; 1212. scanning an avoidance port; 122. a first coupling shaft; 123. a second coupling shaft;

13. a power mechanism; 131. a motor; 132. an output shaft; 133. the component is stirred; 1331. a deflector rod; 1332. a second torsion spring; 134. a fixed block;

14. a reset mechanism; 141. a first torsion spring; 142. a magnetic member;

15. a second limiting member;

16. a bearing;

17. a connecting rod;

18. centrifuging the tube;

19. a control system;

20. a sample gradient separation device;

21. a fixed mount; 211. a load-bearing platform;

22. a second carrier;

23. a third carrier;

24. a transmission mechanism; 241. a first drive pulley; 242. a transmission belt; 243. a poke arm;

25. a first drive mechanism;

26. a detection mechanism;

27. a liquid adding mechanism; 271. an output pipe;

28. a sample adding mechanism;

29. a transfer mechanism; 291. a second drive mechanism.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example one

Fig. 1 to 10 show schematic structural diagrams of a centrifuge tube carrier 10 and a sample gradient separation apparatus 20 according to a first embodiment of the present invention.

Referring to fig. 1 and 4, the centrifuge tube loading apparatus 10 of the present embodiment includes a base 11, a first loading frame 12 and a power mechanism 13. Wherein, the first loading frame 12 is connected with the base 11 in a rotating way; the first loading frame 12 is used for loading the centrifuge tube 18; the power mechanism 13 is used for driving the first loading frame 12 to rotate relative to the base 11, so that the centrifugal tube 18 placed on the first loading frame 12 is in a tilted state. The centrifuging tube carrier device 10 that this embodiment provided, because first bear frame 12 rotates with base 11 to be connected, drive first bear frame 12 through power unit 13, can make first bear frame 12 use the position of being connected with base 11 as the rotation center rotatory, thereby make the centrifuging tube 18 of placing first bear frame 12 in and present the state of slope, be favorable to the sample to sink to centrifuging tube 18 bottom along the orbit of centrifuging tube 18 slope gradually, contain gradient separating medium in centrifuging tube 18 in advance, can make the sample sink along centrifuging tube 18 slope orbit, the extension sinks the distance, thereby can have good separation effect and higher separation efficiency. Referring to fig. 1, 2 and 3, in some embodiments, the base 11 includes a base 111, a first bracket 112 and a second bracket 113. The base 111 has a first side end (not shown) and a second side end (not shown), the first side end and the second side end are opposite to each other, and the first bracket 112 is disposed at the first side end and extends upward from the base 111; the second bracket 113 is provided at the second lateral end and extends upward from the base 111. The base 111, the first bracket 112 and the second bracket 113 are designed such that an area for accommodating the first loading frame 12 is formed between the first bracket 112 and the second bracket 113, thereby facilitating the assembly and disassembly of the first loading frame 12. In some embodiments, the base 111, the first bracket 112, and the second bracket 113 are integrally formed. In some embodiments, the base 111 and the second frame 113 are integrally formed, and the first frame 112 and the base 111 are two detachable components, so as to facilitate the detachment of the first loading frame 12 from the base 11. In some embodiments, the base 111, the first bracket 112, and the second bracket 113 are independent components, and the three components are assembled when in use, and can be disassembled when not in use.

In some embodiments, the first loading ledge 12 is disposed between the first bracket 112 and the second bracket 113, and the first loading ledge 12 is rotatably coupled to the first bracket 112 and the second bracket 113, respectively, such that the first loading ledge 12 can rotate relative to the base 11. In some embodiments, the bottom of the first carrier 12 is spaced from the top of the base 111 to facilitate rotation of the first carrier 12 relative to the base 11.

Referring to fig. 3, in some embodiments, the base 11 is formed with a first limiting member 114, and the first limiting member 114 is connected to the first bracket 112 and/or the second bracket 113. The first limiting member 114 is designed to effectively limit the reset position of the first loading frame 12 during resetting, so as to avoid over-resetting.

In some embodiments, the first position-limiting member 114 is connected to a side of the second bracket 113 away from the rotation direction of the first loading frame 12 (i.e. when the first position-limiting member 114 rotates in a tilting manner of the first loading frame 12, it does not interfere with the rotation of the first loading frame 12, and when the first loading frame 12 returns from the tilting state to the vertical state, the first position-limiting member 114 interferes with the first loading frame 12, so as to prevent the first loading frame 12 from tilting in a reverse direction), and the first position-limiting member 114 extends from the second bracket 113 to the first bracket 112. In some embodiments, the first limiting member 114 is further connected to the base 111. In some embodiments, the first position-limiting member 114 is integrally formed with the base 111. In some embodiments, the first position-limiting member 114 is integrally formed with the base 111 and the second bracket 113. The position of the first limiting member 114 can avoid the first loading frame 12 from interfering with the first limiting member 114 when the first loading frame 12 rotates and tilts, and can avoid the first loading frame 12 from being reset excessively when the first loading frame 12 is reset. In some embodiments, the first stopper 114 is connected to the second bracket 113 near the top of the base 111 to prevent the first stopper 114 from obstructing the scanning mechanism from recognizing and scanning the label attached to the outer wall of the centrifugal tube 18.

Referring to fig. 3, in some embodiments, the centrifuge tube loading apparatus 10 further includes a second limiting member 15, and the second limiting member 15 is disposed on the second bracket 113 and/or a portion of the second bracket 113 away from the bottom end of the base 11 for limiting the rotation range of the first loading frame 12. In some embodiments, the first limiting member 114 and the second limiting member 15 are respectively disposed on two opposite sides of the base 11, the first limiting member 114 and the second limiting member 15 both extend from the first side end portion to the second side end portion, meanwhile, the second limiting member 15 is disposed near the top end of the base 11, the first limiting member 114 is disposed near the bottom end of the base 11, and the second limiting member 15 and the first limiting member 114 are respectively disposed on two opposite sides of the first bearing frame 12 (i.e., the first bearing frame 12 is disposed between the plane where the first limiting member 114 is located and the plane where the second limiting member 15 is located), so that the second limiting member 15 can be used for limiting the inclination range of the first bearing frame 12, and the first limiting member 114 can limit the resetting of the first bearing frame 12. In some embodiments, the second limiting member 15 is configured to limit the rotation range of the first loading frame 12 to 0 ° to 60 °, and if the inclination angle of the first loading frame 12 after rotation is too large, the liquid (such as the gradient separation liquid or the mixture of the gradient separation liquid and the sample) in the centrifuge tube 18 generally occupies about two thirds of the height of the centrifuge tube 18, so that the liquid is easily poured out. In some embodiments, the amplitude of rotation of the first loading ledge 12 is between 0 ° and 45 °.

Referring to fig. 1, 2 and 3, in some embodiments, the first loading frame 12 includes a loading body 121, a first connecting shaft 122 and a second connecting shaft 123. The first connecting shaft 122 and the second connecting shaft 123 are respectively disposed at two opposite side ends of the bearing main body 121, the first connecting shaft 122 and the second connecting shaft 123 are coaxially disposed, and the first connecting shaft 122 extends from the bearing main body 121 to the first bracket 112 to be rotatably connected with the first bracket 112; the second coupling shaft 123 extends from the bearing body 121 toward the second bracket 113 to be rotatably coupled with the second bracket 113. The first loading frame 12 is designed in such a manner that, on one hand, the first loading frame 12 and the base 11 have good connection reliability, and on the other hand, the first connecting shaft 122 and the second connecting shaft 123 are coaxially arranged, so that the first loading frame 12 can rotate relative to the base 11 by taking the central shaft of the first connecting shaft 122 and/or the central shaft of the second connecting shaft 123 as a rotating shaft, and the rotation reliability of the first loading frame 12 is improved.

In some embodiments, the carrying body 121 is formed with a receiving groove 1211, and the notch of the receiving groove 1211 faces upward for placing the centrifugal tube 18. In other embodiments, the carrying body 121 has more than two receiving grooves 1211, each receiving groove 1211 faces upward for receiving the centrifuge tube 18, and two adjacent receiving grooves 1211 are spaced apart from each other. Such a design facilitates loading and unloading of centrifuge tubes 18 into and out of the first carrier 12, and the individual centrifuge tubes 18 are not taken from and placed on the other side of the first carrier 12, which is advantageous for improving the reliability of the centrifuge tube carrier 10. In some embodiments, the plurality of receiving grooves 1211 are spaced along the direction from the first connecting shaft 122 to the second connecting shaft 123, that is, the plurality of receiving grooves 1211 are spaced in a row and a plurality of columns; in some alternative embodiments, the plurality of receiving grooves 1211 may be disposed at intervals in a plurality of rows and columns, or disposed in an array with other shapes.

Referring to fig. 2 and 3, in some embodiments, the supporting body 121 is provided with a scan avoiding port 1212, the scan avoiding port 1212 is disposed on a side wall of the accommodating groove 1211, and the scan avoiding port 1212 is communicated with the accommodating groove 1211 and extends from the notch to a bottom wall of the accommodating groove 1211. In some embodiments, the scan avoidance ports 1212 are all disposed toward the same side. In some embodiments, the scan avoiding openings 1212 are disposed opposite to the second position-limiting members 15, i.e., toward the plane where the first position-limiting members 114 are located. The scan avoidance port 1212 is designed such that when the centrifugal tube 18 placed in the containing groove 1211 rotates about the central axis of the containing groove 1211 as a rotation axis, the label attached to the outer wall of the centrifugal tube 18 is recognized and scanned by the scanning mechanism as the centrifugal tube 18 rotates.

Referring to fig. 3 and 1, in some embodiments, the centrifuge tube carrier 10 further includes a bearing 16, and the bearing 16 is disposed in the base 11 to support the first carrier 12 and rotate the first carrier 12 relative to the base 11. In some embodiments, the number of the bearings 16 is one, and the bearing is embedded in the second bracket 113 to support the second coupling shaft 123. In some embodiments, the number of the bearings 16 is two, and the bearings are respectively disposed on the first bracket 112 and the second bracket 113 to respectively support the first connecting shaft 122 and the second connecting shaft 123. The design of the bearing 16 can effectively reduce the friction force of the first loading frame 12 rotating relative to the base 11, and effectively improve the reliability of the first loading frame 12 rotating relative to the base 11.

Referring to fig. 1 and 3, in some embodiments, the centrifuge tube carrier 10 further includes a connecting rod 17, and the connecting rod 17 is connected to the first carrier frame 12 for the power mechanism 13 to drive the first carrier frame 12 to rotate relative to the base 11. Specifically, one end of the connecting rod 17 is connected to the first loading frame 12, and the other end extends from the first loading frame 12 to the bottom of the base 11, so that the power mechanism 13 drives the connecting rod 17 to rotate the first loading frame 12. In some embodiments, the connecting rod 17 is connected to an end of the second connecting shaft 123 far from the bearing body 121, and the connecting rod 17 is fixedly connected to the second connecting shaft 123, so that when the power mechanism 13 drives the connecting rod 17 to rotate, a central axis of the second connecting shaft 123 of the connecting rod 17 is a rotating axis to rotate, and the first bearing frame 12 is driven to rotate relative to the base 11. In some embodiments, the base 11 is formed with a limiting protrusion 115, the limiting protrusion 115 and the connecting rod 17 are disposed on the same side of the base 11, the limiting protrusion 115 is disposed to limit the movement of the connecting rod 17, and the limiting protrusion 115 extends from the second bracket 113 along the extending direction of the central axis of the second connecting shaft 123. The arrangement of the limiting bulge 115 can avoid excessive resetting of the connecting rod 17 when resetting; on the other hand, it is also possible to avoid that the drive link 17 swings in the opposite direction when the power mechanism 13 is moved against the advance direction, which would lead to a reverse tilting of the first loading carriage 12.

In some embodiments, the connecting rod 17 and the limiting protrusion 115 are disposed on a surface of the second bracket 113 opposite to the first bracket 112. In other embodiments, the connecting rod 17 and the limiting protrusion 115 may also be disposed on the surface of the second bracket 113 facing the first bracket 112. In some embodiments, an avoidance space 110 is formed between the limiting protrusion 115 and the base 111, and the avoidance space 110 is used for providing effective avoidance when the actuating mechanism 13 is fed to push the connecting rod 17 to rotate or when the actuating mechanism 13 moves against the feeding direction.

Referring to fig. 3 and 1, in some embodiments, the centrifuge tube carrier 10 further comprises a reset mechanism 14, and the reset mechanism 14 is disposed on the base 11 and/or the first carrier 12 to reset the centrifuge tube 18 in the tilted state. The tube 18 in the tilted state is returned to the upright position by the return mechanism 14 to avoid interference between two adjacent tube carriers 10 or interference between the tube carriers 10 and other parts during the next process. It should be noted that the reset in the embodiment of the present invention refers to a state that the first loading frame 12 carries the centrifuge tube 18 to rotate relative to the base 11 so that the central axis of the centrifuge tube 18 forms an angle with the vertical direction, and then the first loading frame 12 carries the centrifuge tube 18 to rotate in the opposite direction relative to the base 11 so that the angle between the central axis of the centrifuge tube 18 and the vertical direction becomes smaller until the angle is 0 °.

Referring to fig. 3 and 1, in some embodiments, the return mechanism 14 includes a first torsion spring 141, and the first torsion spring 141 is disposed between the first loading frame 12 and the base 11. When the power mechanism 13 drives the first loading frame 12 to rotate relative to the base 11, the first torsion spring 141 is elastically deformed, and when the power mechanism 13 is separated from the first loading frame 12, the first loading frame 12 carries the centrifugal tube 18 to return under the action of the first torsion spring 141.

Referring to fig. 4, 6 and 12, in some aspects, the power mechanism 13 includes a motor 131, an output shaft 132 and a toggle assembly 133. The output shaft 132 is connected to the motor 131, the toggle assembly 133 is movably connected to the output shaft 132, and the toggle assembly 133 extends toward the first supporting frame 12 to toggle the first supporting frame 12 to rotate relative to the base 11. The power mechanism 13 is configured to drive the toggle assembly 133 to feed by the output shaft 132 so as to push the connecting rod 17 to rotate by taking the central axis of the second connecting shaft 123 as a rotating axis, thereby driving the first loading frame 12 to rotate relative to the base 11; or the output shaft 132 drives the toggle assembly 133 to move in the direction opposite to the feeding direction. In some embodiments, the power mechanism 13 further includes a fixed block 134, the fixed block 134 is fixedly connected to the output shaft 132, and the toggle assembly 133 is disposed on the fixed block 134, so that the fixed block 134 is driven by the output shaft 132 to reciprocate, and the toggle assembly 133 is carried by the fixed block 134 to reciprocate, so that the toggle assembly 133 is fed to push the connecting rod 17 to rotate or is separated from the connecting rod 17 in a direction opposite to the feeding direction.

Referring to fig. 4, 1 and 6, in some embodiments, the centrifuge tube carrier 10 further includes a control system 19. The control system 19 is used for controlling the power mechanism 13 to work. By providing the control system 19, the centrifuge tube carrier 10 can be automated to a high degree.

Referring to fig. 1 to 4 and fig. 5 and 6, based on the centrifuge tube carrying device 10, an embodiment of the invention provides a method for filling a centrifuge tube 18, which includes the following steps:

inserting the centrifuge tube 18 into the first carrier frame 12 of the centrifuge tube carrier 10;

the control system 19 controls the power mechanism 13 to start, so as to control the power mechanism 13 to drive the first loading frame 12 to drive the centrifugal tube 18 to rotate relative to the base 11, so that the central axis of the centrifugal tube 18 forms a preset angle with the vertical direction;

adding the gradient separation liquid into the centrifugal tube 18, and then adding the sample into the centrifugal tube 18 to enable the sample to sink along the extension direction of the wall body of the centrifugal tube 18, so that the sample has a good separation effect;

the first loading frame 12 is rotated by the reset mechanism 14 to reset the centrifuge tube 18 relative to the base 11 until the central axis of the centrifuge tube 18 forms an angle of 0 ° with the vertical.

In some embodiments, the gradient draw solution may be added before the centrifuge tube 18 is inserted into the centrifuge tube carrier 10, after the centrifuge tube 18 is inserted into the centrifuge tube carrier 10, or before or after the center axis of the centrifuge tube 18 is angled at a predetermined angle from vertical. Through adding gradient separation liquid in advance to centrifuging tube 18, add the sample again to centrifuging tube 18, the sample sinks along the extending direction of centrifuging tube 18 wall body at centrifuging tube 18, and the same height of whereabouts, during the centrifuging tube 18 slope, the route of whereabouts is longer than the route of whereabouts when centrifuging tube 18 is vertical to comparatively relaxing, thereby can make the sample have good separation effect and have higher separation efficiency. In some embodiments, the gradient separation liquid and the sample can be added manually or automatically by a machine. When the machine is automatically adding, it is necessary to place the centrifuge tube carrier 10 in the sample gradient separating apparatus 20 as in fig. 5 to 10. In some embodiments, the sample is added in the form of a sample solution or suspension.

Referring to fig. 5 to 10, the embodiment of the invention further provides a sample gradient separation apparatus 20 based on the centrifuge tube carrier 10. In some embodiments, the sample gradient separation apparatus 20 comprises a centrifuge tube carrier 10. In some embodiments, the sample gradient separation apparatus 20 further comprises a fixing frame 21, the fixing frame 21 comprises a carrying platform 211, and the centrifuge tube carrying device 10 is disposed on the top of the carrying platform 211. The centrifuge tube carrier 10 is disposed on the top of the platform 211 for facilitating the addition of the sample into the centrifuge tube 18 received in the first carrier 12 when the first carrier 12 is tilted in the centrifuge tube carrier 10.

Please refer to fig. 5, 6 and 7. In some embodiments, the sample gradient separation apparatus 20 further comprises a second carrier 22, a third carrier 23, a transmission mechanism 24, and a first drive mechanism 25 for driving the transmission mechanism 24. Wherein, the second bears the frame 22 and locates the top of bearing platform 211, through setting up second and bearing frame 22, can be used for bearing the base 11 of centrifuging tube carrier device 10. A transmission mechanism 24 is provided at the bottom of the second carriage 22 for transferring the susceptor 11. The third bearing frame 23 is disposed at the top end of the bearing platform 211 and spaced from the second bearing frame 22, and the third bearing frame 23 can be used for bearing the power mechanism 13 of the centrifuge tube bearing device 10.

In some embodiments, the second carriage 22 has a first end (not shown) and a second end (not shown), and the first end and the second end are disposed opposite to each other, the transmission mechanism 24 extends from the first end to the second end and is disposed at the bottom of the second carriage 22, and the third carriage 23 is disposed near the second end, so that when the transmission mechanism 24 transmits the base 11 disposed at the first end to the second end, the power mechanism 13 disposed at the third carriage 23 can drive the first carriage 12 rotatably connected to the base 11 to rotate relative to the base 11.

Referring to fig. 5 and 7, in some embodiments, the transmission mechanism 24 includes a first transmission wheel 241, a second transmission wheel (not shown), a toggle arm 243, and a transmission belt 242. The first driving wheel 241 and the second driving wheel are respectively disposed at two opposite ends of the second bearing frame 22, for example, the first driving wheel 241 has a first end, the second driving wheel is disposed at a second end, the driving belt 242 is connected between the first driving wheel 241 and the second driving wheel, and the first driving mechanism 25 is connected to the first driving wheel 241 or the second driving wheel to drive one of the driving wheels to drive the other driving wheel to rotate. The toggle arm 243 is connected to the transmission belt 242, and the toggle arm 243 extends upward, which is used to toggle the base 11 disposed on the second carriage 22 to a position where the power mechanism 13 can drive, i.e., to toggle the base 11 disposed on the first end portion to the second end portion. In some alternative embodiments, the operation mode of the transmission mechanism 24 may also be a chain transmission mode, an air cylinder transmission mode, a screw transmission mode, or a rack-and-pinion transmission mode, which may be specifically designed according to actual needs and will not be described herein again.

Please refer to fig. 6 and 10. In some embodiments, the power mechanism 13 includes a motor 131, an output shaft 132, and a toggle assembly 133. Wherein the output shaft 132 is connected with the motor 131, the toggle assembly 133 is movably connected with the output shaft 132, and the toggle assembly 133 is used for toggling the first loading frame 12 to rotate relative to the base 11. In some embodiments, the toggle assembly 133 extends from the third carrier 23 to a region where the second end of the second carrier 22 is located, and when the base 11 is moved to the second end of the second carrier 22, the motor 131 drives the output shaft 132 to drive the toggle assembly 133 to rotate the first carrier 12 relative to the base 11.

In some embodiments, the toggle assembly 133 includes a toggle lever 1331 and a second torsion spring 1332, the second torsion spring 1332 being configured to drive the toggle lever 1331 to return. In some embodiments, the power mechanism 13 further includes a fixed block 134, the fixed block 134 is fixed to the output shaft 132, and the toggle assembly 133 is disposed on the fixed block 134 and is rotatably connected to the fixed block 134. The driving lever 1331 rotates around a portion connected to the fixed block 134 as a rotation center, and the second torsion spring 1332 is disposed at a portion where the driving lever 1331 is connected to the fixed block 134, so that when the driving lever 1331 is forced to rotate around the portion connected to the fixed block 134 as a rotation center axis, the external force disappears, and at this time, the second torsion spring 1332 drives the driving lever 1331 to reset.

Referring to fig. 5, 7 and 9, in some embodiments, the sample gradient separating apparatus 20 further includes a detecting mechanism 26, the detecting mechanism 26 is mounted on the top of the second carriage 22 and is disposed near the first end, and by disposing the detecting mechanism 26, it is possible to detect whether the base 11 mounted with the first carriage 12 is placed on the second carriage 22. In some embodiments, the detection mechanism 26 is a detector, such as a sensor or the like.

Referring to fig. 12, in some embodiments, the control system 19 of the sample gradient separation apparatus 20 is further connected to the first driving mechanism 25 and the detection mechanism 26, respectively, and the control system 19 controls whether the first driving mechanism 25 operates or not according to information fed back by the detection mechanism 26.

Referring to fig. 8, 11, 12 and 13, in some embodiments, the sample gradient separation apparatus 20 further includes a liquid adding mechanism 27 and a liquid adding mechanism 28. Wherein, the liquid adding mechanism 27 is movably connected with the fixed frame 21, and the control system 19 controls the liquid adding mechanism 27 to add the gradient separation liquid into the centrifugal tube 18 arranged in the centrifugal tube bearing device 10; the sample adding mechanism 28 is movably connected to the fixing frame 21, and the control system 19 controls the sample adding mechanism 28 to carry the sample and add the sample into the centrifuge tube 18 placed in the centrifuge tube carrying device 10. In some embodiments, the liquid adding mechanism 27 includes an output tube 271, the liquid outlet end of the output tube 271 is in a conical structure, after the liquid outlet end of the output tube 271 is abutted against the wall body of the centrifugal tube 18, when the output tube 271 and the centrifugal tube 18 are longitudinally cut, an included angle formed by a conical line of the conical structure and the central axis of the output tube 271 is α, an included angle formed by the wall body of the centrifugal tube 18 and the central axis of the output tube 271 is β, and α is smaller than or equal to β, so as to facilitate the gradient separation liquid output from the output tube 271 to flow into the centrifugal tube 18 while being abutted against the wall body of the centrifugal tube 18. Similarly, the relationship between the liquid outlet end of the sample adding mechanism 28 and the centrifuge tube 18 is the same as the relationship between the output tube 271 and the centrifuge tube 18, and the repeated description is omitted here, and the relationship between the liquid outlet end of the sample adding mechanism 28 and the centrifuge tube 18 is the same as the relationship between the output tube 271 and the centrifuge tube 18, so that the separation effect of the sample can be effectively improved.

Referring to fig. 11 and 12, in some embodiments, the sample gradient separating apparatus 20 further includes a transferring mechanism 29 and a second driving mechanism 291, and the second driving mechanism 291 is configured to drive the transferring mechanism 29 for transmission. In some embodiments, the transferring mechanism 29 is used for receiving the base 11 transmitted by the transmission mechanism 24, the power mechanism 13 is arranged at the side of the transferring mechanism 29, and the toggle assembly 131 of the power mechanism 13 extends towards the direction of the transferring mechanism 29 so as to drive the first loading frame 12 to rotate relative to the base 11. In some embodiments, the transfer mechanism 29 is disposed at the second end, and the motion track of the transfer mechanism 29 and the motion track of the transmission mechanism 24 are perpendicular to each other, so that the base 11 travels along the direction from the first end to the second end when being transmitted to the transfer mechanism 29 via the transmission mechanism 24, and is output along the direction perpendicular to the direction from the first end to the second end when being output by the transfer mechanism 29, and is output in the direction away from the power mechanism 13, thereby improving the degree of automation of the sample gradient separation apparatus 20, and improving the efficiency and accuracy of the gradient separation. In some embodiments, the transfer mechanism 29 operates in a chain transmission mode, a belt transmission mode, an air cylinder transmission mode, a screw transmission mode, or a rack-and-pinion transmission mode, which may be specifically set as required and will not be described herein again. In some embodiments, the transfer mechanism 29 includes a finger (not shown) that lifts the base 11 from the bottom of the base 11 and drives the transfer mechanism 29 by the second drive 291 for transfer transport.

Referring to fig. 5 to 13 and fig. 1 to 2, the basic working process of the sample gradient separating apparatus 20 according to the embodiment of the present invention is as follows:

(1) The centrifuge tube 18 is inserted into the first loading frame 12, and the first loading frame 12 is assembled on the base 11, specifically, the centrifuge tube 18 is inserted into the receiving groove 1211 of the first loading frame 12, and the base 11 is placed at the first end of the second loading frame 22.

(2) The first driving mechanism 25 is controlled to drive the transmission mechanism 24 to perform transmission, and the toggle arm 243 toggles the base 11 from the first end portion to the second end portion of the second carriage 22 and moves from the second carriage 22 to the transfer mechanism 29.

(3) The power mechanism 13 is controlled to feed the shift lever 1331 in the direction from the first end to the second end and abut against the connecting rod 17, and the shift lever 1331 continues to feed in the direction from the first end to the second end to drive the connecting rod 17 to rotate relative to the base 11 and drive the first loading frame 12 to rotate the centrifugal tube 18 relative to the base 11, so that the centrifugal tube 18 placed on the first loading frame 12 is tilted, that is: the central axis of the centrifugal tube 18 forms a certain included angle with the vertical direction, and the included angle is 0-60 degrees.

(4) And (4) adding the gradient separation liquid into the centrifuge tube 18 in any time period from the end of the step (2) to the end of the step (3), and enabling the gradient separation liquid to slowly flow to the bottom of the centrifuge tube along the wall body of the centrifuge tube 18 when the gradient separation liquid is added.

(5) Add the sample to centrifuging tube 18 for the sample slowly settles to centrifuging tube 18 bottom along the wall body of centrifuging tube 18, thereby makes the sample have good gradient separation effect, and has higher separation efficiency.

(6) The actuating mechanism 13 is controlled to move in a direction from the second end to the first end (i.e. in a direction opposite to the feeding direction), the lever 1331 is separated from the connecting rod 17, and the first loading frame 12 carries the centrifugal tube 18 to rotate relative to the base 11 under the action of gravity or under the action of the resetting mechanism 14, so as to achieve resetting.

(7) The second driving mechanism 291 is controlled to drive the transfer mechanism 29 to move, so that the transfer mechanism 29 carries the base 11 to output in a direction away from the power mechanism 13.

In some embodiments, since a plurality of bases 11 are moved from the first end to the second end at the same time in step (2), the power mechanism 13 can only drive the first loading frame 12 on the base 11 farthest from the first end to rotate relative to the base 11 at a time, so that another base 11 adjacent to the base 11 farthest from the first end interferes with the movement of the moving mechanism in the direction opposite to the feeding direction of the moving mechanism. For example, the first base 11, the second base 11, the third base 11, … … are arranged in this order from the second end to the first end. After the sample is added into the first base 11 to complete the gradient separation, the power mechanism 13 moves along the direction from the second end to the first end, the first bearing frame 12 of the first base 11 carries the centrifuge tube 18 to reset, and the centrifuge tube 18 is reset and then transferred to the next process by the transfer mechanism 29 together with the first base 11; the link 17 on the second base 11 interferes with the movement of the lever 1331, that is, the link 17 corresponding to the second base 11 applies a force to the lever 1331, so that the lever 1331 rotates around the central rotation axis at the position connected with the fixed block 134, when the power mechanism 13 continues to move in the direction opposite to the feeding direction of the power mechanism 13, the lever 1331 rotating along the link 17 of the second base 11 (that is, the lever 1331 moves from the side of the link 17 of the second base 11 facing the second end portion over the link 17 of the second base 11 and moves to the side of the link 17 of the second base 11 facing the first end portion), and then the lever 1331 is reset by the second torsion spring 1332 to enter the avoiding space 110 of the second base 11 and abuts against the link 17 of the second base 11, at this time, the power mechanism 13 feeds in the direction from the first end portion to the second end portion, so as to push the link 17 of the second base 11 to rotate, thereby driving the first carrier 12 of the second base 11 to rotate relative to the second base 11. The rest of the base 11 can be analogized, and thus, the description thereof will not be repeated.

It should be noted that the sample referred to in this embodiment includes cells to be detected, such as exfoliated cervical cells, and the sample includes the cells to be detected, and the cells to be detected in the sample are obtained by processing the sample, so as to facilitate further processing and observation and analysis of the cells to be detected.

Example two

Referring to fig. 14 and fig. 1 to 3, the difference between the first embodiment and the second embodiment is mainly the structure of the reset mechanism 14. In the first embodiment, the reset mechanism 14 includes a first torsion spring 141, the first torsion spring 141 is disposed between the first loading frame 12 and the base 11, and the first loading frame 12 in the tilted state is driven by the first torsion spring 141 to reset to an angle of 0 ° with the vertical direction. In the embodiment, the reset mechanism 14 includes a magnetic member 142, the magnetic member 142 is disposed on the first loading frame 12 and/or the base 11, and the reset of the centrifuge tube 18 carried by the first loading frame 12 is realized by the magnetic member 142. In some embodiments, the magnetic member 142 is disposed on the first loading frame 12 and is disposed at a position of the first loading frame 12 opposite to the first retaining member 114, and the first retaining member 114 and the magnetic member 142 can generate a magnetic attraction effect to achieve the magnetic attraction restoration of the first loading frame 12. In some embodiments, a part of the magnetic member 142 is disposed on the first loading frame 12, and a part of the magnetic member 142 is disposed on the first retaining member 114, so that the magnetic attraction resetting can be realized. In some embodiments, the magnetic member 142 is disposed on a surface of the first retaining member 114 facing the first loading frame 12 to achieve a magnetically attractive return of the first loading frame 12.

In addition to the above differences, the structure of the centrifuge tube carrier 10 and the sample gradient separation apparatus 20 provided in this embodiment can be optimized with reference to the first embodiment, and will not be described in detail herein.

EXAMPLE III

Referring to fig. 15, fig. 1 to 3, and fig. 14, the difference between the present embodiment and the first and second embodiments is mainly the structure of the reset mechanism 14. In the first embodiment, the resetting mechanism 14 includes a first torsion spring 141, and the first torsion spring 141 is disposed between the first loading frame 12 and the base 11, and the first loading frame 12 in the tilted state is driven to reset to an angle of 0 ° with the vertical direction by the first torsion spring 141; in the second embodiment, the reset mechanism 14 includes a magnetic member 142, the magnetic member 142 is disposed on the first loading frame 12 and/or the base 11, and the reset of the centrifuge tube 18 carried by the first loading frame 12 is realized by the magnetic member 142. In the present embodiment, the reset mechanism 14 includes a first torsion spring 141 and a magnetic member 142; wherein, the first torsion spring 141 is arranged between the first loading frame 12 and the base 11; the magnetic members 142 are provided to the first loading ledge 12 and/or the base 11.

In addition to the above differences, the centrifuge tube carrier 10 and the sample gradient separation apparatus 20 provided in this embodiment can be optimally designed with reference to the first embodiment or the second embodiment, and will not be described in detail herein.

The above is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive various equivalent modifications or substitutions within the technical scope of the present invention, and these modifications or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (17)

1. A centrifugal tube bearing device is characterized by comprising a base, a first bearing frame and a power mechanism;

the first bearing frame is rotatably connected with the base;

the first bearing frame is used for bearing a centrifuge tube;

the power mechanism is used for driving the first bearing frame to rotate relative to the base, so that the centrifugal tube arranged on the first bearing frame is in an inclined state.

2. The centrifuge tube carrier of claim 1, further comprising a reset mechanism disposed on the base and/or the first carrier to reset the centrifuge tube in an inclined state.

3. The centrifuge tube carrier of claim 2, wherein the reset mechanism comprises a first torsion spring disposed between the first carrier and the base;

or, the reset mechanism comprises a magnetic part, and the magnetic part is arranged on the first bearing frame and/or the base;

or the reset mechanism comprises a first torsion spring and a magnetic piece, and the first torsion spring is arranged between the first bearing frame and the base; the magnetic part is arranged on the first bearing frame and/or the base.

4. The centrifuge tube carrier device of any one of claims 1 to 3, wherein the base comprises a base, a first rack, and a second rack; the first bracket and the second bracket are respectively arranged at two opposite ends of the base and extend upwards from the base;

the first bearing frame is arranged between the first support and the second support and is respectively connected with the first support and the second support in a rotating mode.

5. The centrifuge tube carrier of claim 4, wherein the first carrier comprises a carrier body, a first axle and a second axle, the first axle and the second axle being disposed on opposite sides of the carrier body and coaxially disposed; the first connecting shaft is rotatably connected with the first support; the second connecting shaft is rotatably connected with the second support.

6. The centrifuge tube carrying device according to claim 5, wherein the carrying body is formed with a containing groove with a notch facing upward for placing the centrifuge tube;

or, bear the weight of the main part and be formed with the storage tank more than two, every the notch of storage tank is in order to be used for placing up centrifuge tube, adjacent two the storage tank interval sets up.

7. The centrifuge tube carrier device of claim 6, wherein the carrier body is provided with a scan-avoidance port; the scanning avoiding port is arranged on the side wall of the containing groove and communicated with the containing groove.

8. The centrifuge tube carrier device of claim 3, wherein the base is formed with a first stop disposed on the first bracket and/or the second bracket for limiting the reset position of the first carrier.

9. The centrifuge tube carrier device of claim 3, wherein the base is formed with a first stop for limiting the reset position of the first carrier; the centrifuge tube bearing device further comprises a second limiting piece, wherein the second limiting piece is arranged on the second support and/or a part of the second support, which is far away from the bottom end of the base, so as to limit the rotation amplitude of the first bearing frame to be 0-60 degrees; and the first limiting piece and the second limiting piece are respectively arranged on two opposite sides of the first bearing frame.

10. The centrifuge tube carrier of any of claims 1 to 3, further comprising a linkage coupled to the first carrier for rotation of the first carrier relative to the base by the powered mechanism;

or the centrifuge tube carrying device further comprises a connecting rod, and the connecting rod is connected with the first carrying frame so that the power mechanism can drive the first carrying frame to rotate relative to the base; the base is formed with spacing arch, spacing arch with the connecting rod is located the homonymy of base is used for right the swing of connecting rod is spacing.

11. The centrifuge tube carrier device of any one of claims 1 to 3, wherein the power mechanism comprises a motor, an output shaft, and a toggle assembly; the output shaft is connected with the motor, the poking assembly is movably connected with the output shaft and is used for poking the first bearing frame to rotate relative to the base;

and/or, the centrifuge tube bearing device further comprises a control system, and the control system is used for controlling the power mechanism to work.

12. A sample gradient separation apparatus, comprising:

the centrifuge tube carrier of any of claims 1 to 11.

13. The sample gradient separation apparatus of claim 12, further comprising:

a transfer mechanism for holding and transferring the susceptor;

the liquid adding mechanism is used for adding the gradient separation liquid into the centrifuge tube arranged on the centrifuge tube bearing device;

and the sample adding mechanism is used for adding a sample into the centrifugal tube arranged in the centrifugal tube bearing device.

14. The sample gradient separation apparatus according to any of claims 12 to 13, further comprising a second carrier, a third carrier, a transmission mechanism and a first drive mechanism for driving the transmission mechanism;

the second bearing frame is used for bearing the base; the transmission mechanism is used for transmitting the base to the transfer mechanism;

the third bearing frame and the second bearing frame are arranged at intervals and used for bearing the power mechanism.

15. The sample gradient separation apparatus of claim 14, wherein the power mechanism comprises a motor, an output shaft, and a toggle assembly; the output shaft is connected with the motor, and the toggle assembly is movably connected with the output shaft;

and/or the transmission mechanism comprises a poking arm, a first transmission wheel, a second transmission wheel and a transmission belt connected between the first transmission wheel and the second transmission wheel, the first transmission wheel and the second transmission wheel are respectively arranged at two opposite ends of the second bearing frame, the first driving mechanism is used for driving the first transmission wheel or the second transmission wheel to rotate, and the poking arm is connected with the transmission belt; the poking arm is used for poking the base arranged on the second bearing frame to the transfer mechanism so that the power mechanism can drive the first bearing frame which is rotationally connected with the base to rotate relative to the base.

16. A liquid adding method of a centrifugal tube is characterized by comprising the following steps:

inserting a centrifuge tube into the first carrier in the sample gradient separation apparatus of any one of claims 13 to 15;

controlling the power mechanism to drive the first bearing frame to drive the centrifugal tube to rotate relative to the base, so that a preset angle is formed between the central shaft of the centrifugal tube and the vertical direction;

adding a gradient separation solution into the centrifuge tube;

adding a sample into the centrifuge tube;

and controlling the first bearing frame to carry the centrifuge tube to rotate and reset relative to the base until the included angle between the central shaft of the centrifuge tube and the vertical direction is 0 degree.

17. The method for filling a liquid into a centrifuge tube according to claim 16, wherein before controlling the driving of the power mechanism, the method further comprises the steps of placing the base carrying the centrifuge tube on the second carrier, and driving the base to a transfer mechanism by the transmission mechanism;

and/or controlling a liquid adding mechanism to add the gradient separation liquid into the centrifuge tube; and controlling a sample adding mechanism to add the sample into the centrifuge tube.

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