CN115436353A - High-low concentration real-time switching analyzer colorimetric system and using method thereof - Google Patents
High-low concentration real-time switching analyzer colorimetric system and using method thereof Download PDFInfo
- Publication number
- CN115436353A CN115436353A CN202211053604.3A CN202211053604A CN115436353A CN 115436353 A CN115436353 A CN 115436353A CN 202211053604 A CN202211053604 A CN 202211053604A CN 115436353 A CN115436353 A CN 115436353A
- Authority
- CN
- China
- Prior art keywords
- water sample
- adc
- detected
- value
- analyzer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 81
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 29
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 16
- 238000006243 chemical reaction Methods 0.000 claims abstract description 12
- 230000029087 digestion Effects 0.000 claims abstract description 10
- 230000005540 biological transmission Effects 0.000 claims abstract description 9
- 238000001514 detection method Methods 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 67
- 239000012086 standard solution Substances 0.000 description 19
- 238000004737 colorimetric analysis Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 9
- 238000005259 measurement Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000000007 visual effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 238000001429 visible spectrum Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/20—Controlling water pollution; Waste water treatment
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Engineering & Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention provides an analyzer colorimetric system with high and low concentration real-time switching and a using method thereof, wherein the system comprises: the device comprises a peristaltic pump and a multi-way valve, wherein the multi-way valve is respectively connected with a meter, a digestion tank, a colorimetric device and a plurality of reagent bottles, and the peristaltic pump is connected with the meter; the controller is connected with the data transmission circuit, the data transmission circuit is connected with the MCU, the MCU is connected with the ADC, the ADC is connected with the photoelectric conversion circuit, and the photoelectric conversion circuit is connected with the color comparator; wherein, the colorimetric device includes cell, light emitter and photoreceiver, and light emitter, photoreceiver are connected respectively to the cell, and when the cell was in the primary importance, cell and light emitter, photoreceiver were perpendicular, and when the cell was in the second place, cell and light emitter, photoreceiver were parallel. The analyzer colorimetric system capable of switching high and low concentrations in real time and the using method thereof improve the detection precision of a water sample to be detected in a high range by judging whether the low range or the high range is used.
Description
Technical Field
The invention relates to the technical field of water quality monitoring equipment in the field of environmental protection, in particular to an analyzer colorimetric system with high and low concentration switching in real time and a using method thereof.
Background
In the prior art, the Colorimetry (Colorimetry) is a method for determining the content of a component to be measured by comparing or measuring the color depth of a colored substance solution. Two types of colorimetric methods are commonly used: visual colorimetry and photoelectric colorimetry, both of which are based on the lambert-beer law. The common visual colorimetry is a standard series method, that is, different amounts of standard solutions of an object to be measured are put into a group of identical colorimetric tubes, and color development is carried out according to the analysis steps to prepare a standard color gradation with gradually changing color. The sample solution is developed under the same condition, and compared with the standard color gradation, the standard with the most similar color is visually found out, and the content of the component to be measured in the sample is calculated and determined according to the amount of the standard solution contained in the standard solution. Compared with a visual colorimetry, the photoelectric colorimetry eliminates subjective errors, improves measurement accuracy, and can eliminate interference by selecting an optical filter, so that selectivity is improved. However, the photoelectric colorimeter adopts a tungsten lamp light source and an optical filter, is only suitable for a visible spectrum region and can only obtain composite light with a certain wavelength range, but not monochromatic light beams.
In the water quality monitoring, the contents of substances such as ammonia nitrogen, total phosphorus, total nitrogen and the like are measured by adopting a photoelectric colorimetric method. However, when a photoelectric colorimetry is used for measuring a large range, the measurement cannot be accurately carried out on less than 20% of the range, and the range switching is necessary, so that the current range switching has many disadvantages: 1. blind measurement is needed to judge whether the water sample is in a high-range or a low-range at one time, so that reagents are wasted, and the measurement time is increased; 2. often, the test time required for switching the measuring range is more than 1 hour, so that the test cannot be completed within 1 hour of the instrument, and the latest requirements of the environment-friendly industry cannot be met. Colorimetric high-range tests do not meet the requirement of a readout error (+ -3%). The high range needs to dilute the water sample, and the more the number of times of dilution is, the larger the test error is. The larger the measurement concentration of the high range is, the light transmittance approaches to 0%, and the absorbance tends to infinity, so that effective quantification cannot be performed.
Therefore, there is a need to provide an analyzer colorimetric system capable of switching between high and low concentrations in real time and a method for using the same, so as to effectively solve the above problems.
Disclosure of Invention
The invention provides an analyzer colorimetric system with high and low concentration switching in real time and a using method thereof, which improve the detection precision of a water sample to be detected in a high range by judging whether the water sample is used in a low range or a high range.
The embodiment of the invention provides an analyzer colorimetric system with high and low concentrations switched in real time, which comprises:
the device comprises a peristaltic pump and a multi-way valve, wherein the multi-way valve is respectively connected with a meter, a digestion tank, a colorimetric device and a plurality of reagent bottles, and the peristaltic pump is connected with the meter;
the controller is connected with the data transmission circuit, the data transmission circuit is connected with the MCU, the MCU is connected with the ADC, the ADC is connected with the photoelectric conversion circuit, and the photoelectric conversion circuit is connected with the color comparator;
the colorimetric device comprises a cuvette, a light emitter and a light receiver, wherein the cuvette is respectively connected with the light emitter and the light receiver, the cuvette has a first position and a second position, when the cuvette is at the first position, the cuvette is vertical to the light emitter and the light receiver, and when the cuvette is at the second position, the cuvette is parallel to the light emitter and the light receiver.
Preferably, the controller controls an on-off valve that opens the multi-way valve, through which reagent in one of the plurality of reagent bottles passes into the multi-way valve and then into the meter.
Preferably, a plurality of infrared stoppers are provided on the meter, and when at least two of the plurality of infrared stoppers detect the reagent, the peristaltic pump stops and the meter meters the reagent.
Preferably, the system further comprises a rotating motor, the controller is connected with the rotating motor, the cuvette is fixed on a rotating disc, and the rotating disc is fixed on a working rod of the rotating motor; and the controller controls the rotating motor to rotate, so that the rotating disc is driven to rotate, and the cuvette is driven to rotate to the first position or the second position.
Preferably, the cuvette further comprises a colorimetric support for fixing the light emitter, the light receiver and the rotating motor.
Preferably, the system further comprises a touch screen, the touch screen is connected with the controller, and the touch screen is used for displaying the position of the rotating motor.
Preferably, the cuvette is a cuboid, the cuvette being of different length and width.
The embodiment of the invention also provides a using method of the analyzer colorimetric system suitable for the high-low concentration real-time switching, wherein the using method comprises the following steps:
measuring a water sample to be detected, digesting the water sample to be detected, and then feeding the digested water sample into the cuvette;
respectively locating the cuvettes at the first position and the second position, and respectively calculating the concentration value of the water sample to be detected through a first formula and a second formula;
when the concentration value of the water sample to be detected is less than or equal to a first standard value, calculating by a first formula to obtain the concentration value of the water sample to be detected;
and when the concentration value of the water sample to be detected is greater than the first standard value and less than or equal to the second standard value, calculating to obtain the concentration value of the water sample to be detected through a second formula.
Preferably, the first formula is specifically as follows:
wherein, C x For the concentration value of the water sample to be measured, ADC 01 For zero calibration of the value converted by the transverse ADC, ADC 1 For low-density calibration of the converted values of the transverse ADC, ADC x And the value is the value of the water sample to be detected after ADC conversion.
Preferably, the second formula is specifically as follows:
wherein, C x For the concentration value of the water sample to be measured, ADC 02 For the values converted by the longitudinal ADC during zero calibration, ADC 2 For high concentration calibration of the converted values of the vertical ADC, ADC x The value is the value converted by the ADC of the water sample to be detected.
The technical scheme of the embodiment of the invention has the following beneficial effects:
the analyzer colorimetric system with high and low concentration real-time switching and the using method thereof provided by the embodiment of the invention are characterized in that the cuvette is respectively connected with the light emitter and the light receiver, the cuvette has a first position and a second position, when the cuvette is at the first position, the cuvette is vertical to the light emitter and the light receiver, and when the cuvette is at the second position, the cuvette is parallel to the light emitter and the light receiver, so that a water sample to be measured can be conveniently measured transversely and longitudinally;
further, measuring a water sample to be detected, digesting the water sample to be detected, and then feeding the digested water sample into the cuvette; respectively locating the cuvettes at the first position and the second position, and respectively calculating the concentration value of the water sample to be detected through a first formula and a second formula; when the concentration value of the water sample to be detected is less than or equal to a first standard value, calculating by a first formula to obtain the concentration value of the water sample to be detected; when the concentration value of the water sample to be detected is larger than the first standard value and smaller than or equal to the second standard value, the concentration value of the water sample to be detected is obtained through calculation of the second formula, so that whether a low range or a high range is used can be determined by judging the concentration value of the water sample to be detected, and the detection precision of the water sample to be detected in the high range is improved.
Drawings
To more clearly illustrate the embodiments or prior art solutions of the present invention, a brief description will be given below of the drawings required for describing the embodiments or prior art, and it is apparent that the drawings in the following description are some embodiments, not all embodiments, of the present invention. For a person skilled in the art, without inventive step, other figures can also be obtained from these figures.
FIG. 1 is a schematic diagram of a high-low concentration real-time switching analyzer colorimetric system according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the control relationship of an analyzer colorimetric system with real-time switching of high and low concentrations according to another embodiment of the present invention;
fig. 3 is a schematic diagram of a cuvette of a high-low concentration real-time switching analyzer colorimetric system according to an embodiment of the present invention;
fig. 4 is a schematic flow chart of a method for using the analyzer colorimetric system with high and low concentration switching in real time according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The technical solution of the present invention will be described in detail below with specific examples. The following several specific embodiments may be combined with each other, and details of the same or similar concepts or processes may not be repeated in some embodiments.
Based on the problems in the prior art, the embodiment of the invention provides an analyzer colorimetric system with high and low concentration switching in real time and a using method thereof, and the detection precision of a water sample to be detected in a high range is improved by judging whether the low range or the high range is used.
Fig. 1 is a schematic structural diagram of an analyzer colorimetric system with high and low concentration switching in real time according to an embodiment of the present invention, fig. 2 is a schematic control relationship diagram of an analyzer colorimetric system with high and low concentration switching in real time according to another embodiment of the present invention, fig. 3 is a schematic structural diagram of a cuvette of an analyzer colorimetric system with high and low concentration switching in real time according to an embodiment of the present invention, and referring now to fig. 1 to fig. 3, an embodiment of the present invention provides an analyzer colorimetric system with high and low concentration switching in real time, where the system includes: the device comprises a peristaltic pump 1 and a multi-way valve 3, wherein the multi-way valve 3 is respectively connected with a meter 2, a digestion tank 4, a colorimetric device 5 and a plurality of reagent bottles 6, and the peristaltic pump 1 is connected with the meter 2; the controller 19 is connected with the data transmission circuit 10, the data transmission circuit 10 is connected with the MCU9, the MCU9 is connected with the ADC converter 8, the ADC converter 8 is connected with the photoelectric conversion circuit 7, and the photoelectric conversion circuit 7 is connected with the colorimetric device 5; wherein the cuvette 5 comprises a cuvette 16, a light emitter 12 and a light receiver 13, the cuvette 16 is connected to the light emitter 12 and the light receiver 13, respectively, the cuvette 16 has a first position and a second position, when the cuvette 16 is in the first position, the cuvette 16 is perpendicular to the light emitter 12 and the light receiver 13, and when the cuvette 16 is in the second position, the cuvette 16 is parallel to the light emitter 12 and the light receiver 13. The first position is a vertical position and the second position is a horizontal position.
In a specific implementation, the controller 19 controls an on-off valve that opens the multi-way valve 2, through which reagent in one of the plurality of reagent bottles 6 passes into the multi-way valve 2 and then into the meter 2.
In a specific implementation, the meter 2 is provided with a plurality of infrared stoppers, when at least two of the plurality of infrared stoppers detect the reagent, the peristaltic pump 1 stops, and the meter 2 meters the reagent.
In a specific implementation, the system further comprises a rotating motor 14, the controller 19 is connected with the rotating motor 14, the cuvette 16 is fixed on a rotating disc 15, and the rotating disc 15 is fixed on a working rod of the rotating motor 14; when the controller 19 controls the rotating motor 14 to rotate, the rotating disc 15 is driven to rotate, and then the cuvette 16 is driven to rotate to the first position or the second position.
In a specific implementation, the colorimetric device 5 further includes a colorimetric support 11, and the colorimetric support 11 is configured to fix the light emitter 12, the light receiver 13, and the rotating motor 14.
In a specific implementation, the system further includes a touch screen 20, the touch screen 20 is connected to the controller 19, and the touch screen 20 is used for displaying the position of the rotating motor 14.
In a specific implementation, the cuvette 16 is a rectangular parallelepiped, and the cuvette 16 has different lengths and widths.
In particular use, the peristaltic pump 1 aspirates one of the plurality of reagent bottles 6, and draws reagent from one of the reagent bottles 6 through the multi-way valve 3 and into the meter 2 for metering. For example, the peristaltic pump 1 pumps one of the reagent bottles 6, the controller 19 controls the opening and closing valve on the corresponding multi-way valve 3 to open, the reagent in one of the reagent bottles 6 enters the multi-way valve 3 through the opening and closing valve and then enters the meter 2, more than two infrared limiters on the meter 2 detect the reagent, signals are detected and the metering is carried out through different infrared limiters, the peristaltic pump 1 stops, and then the reagent amount can be metered. Thereafter, the peristaltic pump 1 is reversed to force out the meter 2, and at the same time, the controller 19 controls the on-off valve of the multi-way valve 3, thereby pressing the reagent into the digestion tank 4. Firstly, adding an oxidant reagent A into a digestion tank 4 for digestion, digesting a water sample by a heating device of the digestion tank 4, and sequentially adding a buffer reagent B and a color developing agent reagent C after digestion is finished for color development reaction. Then, the peristaltic pump 1 pushes the digested water sample in the digestion tank 4 into the cuvette 16 of the colorimetric device 5.
The light emitter 12 and the light receiver 13 of the colorimetric device 5 perform colorimetric comparison on a water sample in the colorimetric dish 16, and the colorimetric signals sequentially pass through the photoelectric conversion circuit 7, the ADC converter 8, the MCU9 and the data transmission circuit 10, and finally the ADC numerical signals are sent to the controller 19 and displayed through the touch screen 20.
The embodiment of the invention also provides a using method of the analyzer colorimetric system suitable for the high-low concentration real-time switching, wherein the using method comprises the following steps:
step 401: measuring a water sample to be measured, and digesting the water sample to be measured and then feeding the water sample to be measured into the cuvette;
step 402: respectively locating the cuvettes at the first position and the second position, and respectively calculating the concentration value of the water sample to be detected through a first formula and a second formula;
step 403: when the concentration value of the water sample to be detected is less than or equal to a first standard value, calculating by a first formula to obtain the concentration value of the water sample to be detected;
step 404: and when the concentration value of the water sample to be detected is greater than the first standard value and less than or equal to the second standard value, calculating to obtain the concentration value of the water sample to be detected through a second formula.
The first standard value is the concentration value of the standard solution 1, and the second standard value is the concentration value of the standard solution 2. When the device is used, three standard solutions 1, 2 and 3 are adopted to calibrate a system, wherein the standard solution 1 is a low-concentration water sample with a known concentration value, the standard solution 2 is a high-concentration water sample with a known concentration value, and the standard solution 3 is 0 mol.L -1 。
In a specific implementation, the first formula is specifically as follows:
wherein, C x For the concentration value of the water sample to be measured, ADC 01 For zero calibration of the converted value of the transverse ADC, ADC 1 For low-density calibration of the converted values of the transverse ADC, ADC x Is that theAnd D, converting the value of the water sample to be detected by the ADC.
In a specific implementation, the second formula is specifically as follows:
wherein, C x For the concentration value of the water sample to be measured, ADC 02 For the values converted by the longitudinal ADC during zero calibration, ADC 2 For high concentration calibration of the converted values of the vertical ADC, ADC x The value is the value converted by the ADC of the water sample to be detected.
When the device is used, the standard solution 1 and the standard solution 3 are combined, the cuvette 16 is respectively parallel to the light emitter 12 and the light receiver 13, namely the cuvette 16 is in a first position, namely a transverse position, and a first formula can be obtained through calibration.
The standard solution 2 and the standard solution 3 are combined, the cuvette 16 is perpendicular to the optical emitter 12 and the optical receiver 13 respectively, namely the cuvette 16 is in a second position, namely a vertical position, and a second formula can be obtained through calibration.
After the calibration is completed, a water sample to be measured is measured, the water sample to be measured enters the cuvette 16 after being digested, the cuvette 16 is respectively placed at a second position, namely a vertical position, and a first position, namely a horizontal position, the first formula and the second formula are respectively substituted to calculate the concentration value of the water sample to be measured, when the concentration value of the water sample to be measured is between the concentration value of standard solution 3 and the concentration value of standard solution 1, namely between the concentration value of standard solution 0 and the concentration value of standard solution 1, namely when the water sample to be measured is low in concentration, the first formula is substituted to calculate the concentration value of the water sample to be measured, when the concentration value of the water sample to be measured is between the concentration value of standard solution 1 and the concentration value of standard solution 2, namely when the water sample to be measured is high in concentration, the second formula is substituted to calculate the concentration value of the water sample to be measured, and the concentration value measurement of the water sample to be measured is completed.
In summary, in the analyzer colorimetric system with high and low concentration switching in real time and the using method thereof provided by the embodiments of the present invention, the cuvette is respectively connected to the light emitter and the light receiver, the cuvette has a first position and a second position, when the cuvette is in the first position, the cuvette is perpendicular to the light emitter and the light receiver, and when the cuvette is in the second position, the cuvette is parallel to the light emitter and the light receiver, so that transverse and longitudinal measurements can be conveniently performed on a water sample to be measured;
further, measuring a water sample to be detected, and digesting the water sample to be detected and then feeding the water sample to be detected into the cuvette; respectively locating the cuvettes at the first position and the second position, and respectively calculating the concentration value of the water sample to be detected through a first formula and a second formula; when the concentration value of the water sample to be detected is less than or equal to a first standard value, calculating by a first formula to obtain the concentration value of the water sample to be detected; when the concentration value of the water sample to be detected is larger than the first standard value and smaller than or equal to the second standard value, the concentration value of the water sample to be detected is obtained through calculation of the second formula, so that whether a low range or a high range is used can be determined by judging the concentration value of the water sample to be detected, and the detection precision of the water sample to be detected in the high range is improved.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. An analyzer colorimetric system capable of switching between high and low concentrations in real time, the system comprising:
the device comprises a peristaltic pump and a multi-way valve, wherein the multi-way valve is respectively connected with a meter, a digestion tank, a colorimetric device and a plurality of reagent bottles, and the peristaltic pump is connected with the meter;
the controller is connected with the data transmission circuit, the data transmission circuit is connected with the MCU, the MCU is connected with the ADC, the ADC is connected with the photoelectric conversion circuit, and the photoelectric conversion circuit is connected with the color comparator;
wherein, the colorimetric device includes cell, light emitter and light receiver, the cell is connected respectively the light emitter the light receiver, the cell has primary importance and second place, works as the cell is in when primary importance, the cell with the light emitter the light receiver is perpendicular, works as the cell is in when the second place, the cell with the light emitter the light receiver is parallel.
2. The high-low concentration real-time switching analyzer colorimetric system of claim 1, wherein the controller controls an on-off valve that opens the multi-way valve, and reagent in one of the plurality of reagent bottles passes through the on-off valve into the multi-way valve and then into the meter.
3. The high-low concentration real-time switching analyzer colorimetric system of claim 1, wherein the meter is provided with a plurality of infrared stoppers, and when at least two of the plurality of infrared stoppers detect the reagent, the peristaltic pump is stopped, and the meter meters the reagent.
4. The analyzer colorimetric system for real-time switching of high and low concentrations according to claim 1, further comprising a rotating motor, wherein the controller is connected to the rotating motor, the cuvette is fixed on a rotating disk, and the rotating disk is fixed on a working rod of the rotating motor; and the controller controls the rotating motor to rotate, so that the rotating disc is driven to rotate, and the cuvette is driven to rotate to the first position or the second position.
5. The high-low concentration real-time switching analyzer colorimetric system of claim 4, wherein the colorimetric device further comprises a colorimetric support for fixing the light emitter, the light receiver, and the rotary motor.
6. The high-low concentration real-time switching analyzer colorimetric system of claim 4, further comprising a touch screen connected to the controller, wherein the touch screen is used for displaying the position of the rotating motor.
7. The high-low concentration real-time switching analyzer colorimetric system according to claim 1, wherein the cuvette is a rectangular parallelepiped, and the cuvette is different in length and width.
8. A method for using the analyzer colorimetric system suitable for switching between high and low concentrations in real time according to any one of claims 1 to 7, wherein the method for using the analyzer colorimetric system comprises:
measuring a water sample to be detected, digesting the water sample to be detected, and then feeding the digested water sample into the cuvette;
respectively locating the cuvettes at the first position and the second position, and respectively calculating the concentration value of the water sample to be detected through a first formula and a second formula;
when the concentration value of the water sample to be detected is less than or equal to a first standard value, calculating by a first formula to obtain the concentration value of the water sample to be detected;
and when the concentration value of the water sample to be detected is greater than the first standard value and less than or equal to the second standard value, calculating to obtain the concentration value of the water sample to be detected through a second formula.
9. Use according to claim 8, characterized in that: the first formula is specifically as follows:
wherein, C x For the concentration value of the water sample to be measured, ADC 01 For zero calibration of the value converted by the transverse ADC, ADC 1 For low-density calibration of the converted values of the transverse ADC, ADC x And the value is the value of the water sample to be detected after ADC conversion.
10. Use according to claim 8, characterized in that: the second formula is specifically as follows:
wherein, C x For the concentration value of the water sample to be measured, ADC 02 For the values converted by the longitudinal ADC during zero calibration, ADC 2 For high concentration calibration of the converted values of the vertical ADC, ADC x The value is the value converted by the ADC of the water sample to be detected.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211053604.3A CN115436353B (en) | 2022-08-31 | 2022-08-31 | High-low concentration real-time switching analyzer colorimetric system and use method thereof |
| PCT/CN2023/115865 WO2024046372A1 (en) | 2022-08-31 | 2023-08-30 | Analyzer colorimetric system capable of switching high concentration and low concentration in real time and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211053604.3A CN115436353B (en) | 2022-08-31 | 2022-08-31 | High-low concentration real-time switching analyzer colorimetric system and use method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115436353A true CN115436353A (en) | 2022-12-06 |
| CN115436353B CN115436353B (en) | 2023-06-30 |
Family
ID=84244358
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211053604.3A Active CN115436353B (en) | 2022-08-31 | 2022-08-31 | High-low concentration real-time switching analyzer colorimetric system and use method thereof |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN115436353B (en) |
| WO (1) | WO2024046372A1 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115326725A (en) * | 2022-09-13 | 2022-11-11 | 上海博取仪器有限公司 | Chemical oxygen demand detection system with automatic switching of high and low measuring ranges |
| CN115931451A (en) * | 2023-03-10 | 2023-04-07 | 中绿环保科技股份有限公司 | Sampling analysis unit suitable for surface water monitoring |
| CN116609279A (en) * | 2023-05-29 | 2023-08-18 | 上海博取仪器有限公司 | Calibrating device of water quality testing equipment light source |
| CN116840219A (en) * | 2023-07-31 | 2023-10-03 | 上海博取仪器有限公司 | Method for detecting total nitrogen concentration of water quality |
| WO2024046372A1 (en) * | 2022-08-31 | 2024-03-07 | 上海博取仪器有限公司 | Analyzer colorimetric system capable of switching high concentration and low concentration in real time and use method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050185177A1 (en) * | 2004-02-23 | 2005-08-25 | Moran Donald J.Jr. | Determining an analyte by multiple measurements through a cuvette |
| CN203011829U (en) * | 2012-11-20 | 2013-06-19 | 北京雪迪龙科技股份有限公司 | Cuvette, water quality concentration detecting device and water quality monitoring system |
| CN204495711U (en) * | 2015-04-03 | 2015-07-22 | 中兴仪器（深圳）有限公司 | A kind of concentration measurements device |
| CN204758475U (en) * | 2015-07-07 | 2015-11-11 | 烟台东润仪表有限公司 | Online ammonia nitrogen analysis appearance of two optical distance analytical approachs |
| CN207636479U (en) * | 2017-11-24 | 2018-07-20 | 南瑞集团有限公司 | A kind of resolution colorimetric pool with flush end optical measurement window |
| CN110646383A (en) * | 2018-06-26 | 2020-01-03 | 山东思睿环境设备科技有限公司 | Water quality total cobalt online monitoring device and monitoring method |
| CN215640837U (en) * | 2021-09-14 | 2022-01-25 | 深圳市清时捷科技有限公司 | Micro multi-optical path flow colorimetric device |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080293091A1 (en) * | 2007-05-25 | 2008-11-27 | Ravi Kanipayor | Apparatus and methods for automated diffusion filtration, culturing and photometric detection and enumeration of microbiological parameters in fluid samples |
| CN201993318U (en) * | 2011-04-02 | 2011-09-28 | 南通汇环环保科技有限公司 | Ammonia nitrogen online automatic monitor |
| CN106442349A (en) * | 2016-09-14 | 2017-02-22 | 南京南瑞集团公司 | Colorimetric measuring device and use method thereof |
| US11237179B2 (en) * | 2017-10-31 | 2022-02-01 | Canon Medical Systems Corporation | Calibration curve generating method and automatic analyzing apparatus |
| CN115436353B (en) * | 2022-08-31 | 2023-06-30 | 上海博取仪器有限公司 | High-low concentration real-time switching analyzer colorimetric system and use method thereof |
-
2022
- 2022-08-31 CN CN202211053604.3A patent/CN115436353B/en active Active
-
2023
- 2023-08-30 WO PCT/CN2023/115865 patent/WO2024046372A1/en unknown
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050185177A1 (en) * | 2004-02-23 | 2005-08-25 | Moran Donald J.Jr. | Determining an analyte by multiple measurements through a cuvette |
| CN203011829U (en) * | 2012-11-20 | 2013-06-19 | 北京雪迪龙科技股份有限公司 | Cuvette, water quality concentration detecting device and water quality monitoring system |
| CN204495711U (en) * | 2015-04-03 | 2015-07-22 | 中兴仪器（深圳）有限公司 | A kind of concentration measurements device |
| CN204758475U (en) * | 2015-07-07 | 2015-11-11 | 烟台东润仪表有限公司 | Online ammonia nitrogen analysis appearance of two optical distance analytical approachs |
| CN207636479U (en) * | 2017-11-24 | 2018-07-20 | 南瑞集团有限公司 | A kind of resolution colorimetric pool with flush end optical measurement window |
| CN110646383A (en) * | 2018-06-26 | 2020-01-03 | 山东思睿环境设备科技有限公司 | Water quality total cobalt online monitoring device and monitoring method |
| CN215640837U (en) * | 2021-09-14 | 2022-01-25 | 深圳市清时捷科技有限公司 | Micro multi-optical path flow colorimetric device |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024046372A1 (en) * | 2022-08-31 | 2024-03-07 | 上海博取仪器有限公司 | Analyzer colorimetric system capable of switching high concentration and low concentration in real time and use method thereof |
| CN115326725A (en) * | 2022-09-13 | 2022-11-11 | 上海博取仪器有限公司 | Chemical oxygen demand detection system with automatic switching of high and low measuring ranges |
| CN115326725B (en) * | 2022-09-13 | 2024-05-14 | 上海博取仪器有限公司 | Chemical oxygen demand detecting system capable of automatically switching high range and low range |
| CN115931451A (en) * | 2023-03-10 | 2023-04-07 | 中绿环保科技股份有限公司 | Sampling analysis unit suitable for surface water monitoring |
| CN115931451B (en) * | 2023-03-10 | 2023-09-01 | 中绿环保科技股份有限公司 | Sampling analysis unit suitable for surface water monitoring |
| CN116609279A (en) * | 2023-05-29 | 2023-08-18 | 上海博取仪器有限公司 | Calibrating device of water quality testing equipment light source |
| CN116609279B (en) * | 2023-05-29 | 2023-12-22 | 上海博取仪器有限公司 | Calibrating device of water quality testing equipment light source |
| CN116840219A (en) * | 2023-07-31 | 2023-10-03 | 上海博取仪器有限公司 | Method for detecting total nitrogen concentration of water quality |
| CN116840219B (en) * | 2023-07-31 | 2024-03-19 | 上海博取仪器有限公司 | Method for detecting total nitrogen concentration of water quality |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115436353B (en) | 2023-06-30 |
| WO2024046372A1 (en) | 2024-03-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN115436353A (en) | High-low concentration real-time switching analyzer colorimetric system and using method thereof | |
| US11454619B2 (en) | Methods for colorimetric endpoint detection and multiple analyte titration systems | |
| JPH0134337B2 (en) | ||
| WO2024046369A1 (en) | Anti-interference water quality analysis instrument test method | |
| CN205484004U (en) | Adopt device of resolution method on -line monitoring quality of water multi -parameter | |
| WO2021258552A1 (en) | Timed test system for water quality test device | |
| US8928884B2 (en) | Validation method for automated analyzers | |
| US9594088B2 (en) | Multiple time windows with associated calibration curves for extending the range of an assay | |
| JPH06308131A (en) | Data processing apparatus | |
| CN110987845A (en) | Real-time detection method for dye concentration in single-component and double-component dye solutions | |
| CN110208260A (en) | Potassium bromate quick detection reagent, kit and detection method in a kind of flour | |
| JP3168633B2 (en) | Prozone determination method and analysis method in antigen-antibody reaction | |
| CN106442349A (en) | Colorimetric measuring device and use method thereof | |
| US10222322B2 (en) | Colorimetric analyzer with improved error detection | |
| CN112257017A (en) | Unitary linear point-by-point analysis method, system and device of standardized residual error detection method | |
| CN104330403A (en) | Method for measuring sulphurous acid radical based on kinetic spectrophotometry | |
| CN108535498A (en) | Aluminium automatic analysing apparatus and analysis method in water | |
| JPS6118982B2 (en) | ||
| CN103674861A (en) | Measuring method of absorbance | |
| CN110987912A (en) | Analysis and test method for urea content in raw milk | |
| JP2508115B2 (en) | Automatic biochemical analyzer | |
| Liedtke et al. | Centrifugal analysis with automated sequential reagent addition: measurement of serum calcium. | |
| CN107389575A (en) | A kind of rotten automatic testing method of reagent and system | |
| CN118090662A (en) | Rapid detection method and device for soft sugar | |
| Crowther et al. | Automatic determination of inorganic carbon in surface waters |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A real-time switching analyzer colorimetric system for high and low concentration and its usage method Effective date of registration: 20231219 Granted publication date: 20230630 Pledgee: Industrial Bank Co.,Ltd. Shanghai pilot Free Trade Zone Lingang New District sub branch Pledgor: SHANGHAI BOQU INSTRUMENT CO.,LTD. Registration number: Y2023310000889 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |