CN115428785A - Oxygen injection perfusion system for isolated organ and operation method - Google Patents
Oxygen injection perfusion system for isolated organ and operation method Download PDFInfo
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- CN115428785A CN115428785A CN202211041013.4A CN202211041013A CN115428785A CN 115428785 A CN115428785 A CN 115428785A CN 202211041013 A CN202211041013 A CN 202211041013A CN 115428785 A CN115428785 A CN 115428785A
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- oxygen
- adsorption tower
- adsorption
- molecular sieve
- perfusion system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0247—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B13/00—Oxygen; Ozone; Oxides or hydroxides in general
- C01B13/02—Preparation of oxygen
Abstract
The invention provides an oxygen injection perfusion system for an isolated organ and an operation method thereof. The molecular sieve oxygen generation unit is provided with a plurality of adsorption towers which can alternately work, and can realize continuous oxygen injection at any time.
Description
Technical Field
The invention belongs to the field of biomedical instruments, and particularly relates to an isolated organ perfusion system with an oxygen injection function and an operation method.
Background
Organ transplantation refers to the surgical transfer of a viable isolated organ into a patient. The temperature must be controlled in a low-temperature environment of 2-8 ℃ during organ storage, and the traditional organ storage method is static cold storage. The longer the static cold storage time, the higher the incidence of delayed recovery of graft function after surgery, and in addition, the difficulty in objective assessment of isolated organs by static cold storage. Compared with static cold preservation, the low-temperature mechanical perfusion of the isolated organ can obviously reduce the incidence rate of delayed recovery of the function of the transplanted organ, is beneficial to improving the long-term survival rate of the transplanted organ, and meanwhile, the mechanical perfusion parameters can assist in evaluating the quality of the transplanted organ and are beneficial to the full utilization of the isolated organ. The organ low-temperature perfusion preservation box is equipment for transferring and preserving the isolated organ for transplantation and low-temperature mechanical perfusion, can prolong the storage time of the isolated organ, evaluate the quality of the isolated organ and reduce the delayed recovery rate of the function of the postoperative graft, and is widely applied at present.
When the isolated organ is perfused with perfusate, the oxygen concentration in the perfusate is usually required to be kept at a certain level, so that the aerobic metabolism of the isolated organ can be supported, the antioxidant is increased, the mitochondrial function is recovered, and the delayed function recovery is reduced. Hypoxia metabolism may lead to anaerobic activity in organ cells, resulting in the release and accumulation of toxic molecules such as free radical oxygen species, inflammatory cytokines and lactate, exacerbating ischemia and reperfusion injury. Perfusion apparatuses currently on the market are therefore generally provided with oxygenation means arranged to add oxygen to the perfusion fluid as it circulates in the perfusion circuit. At present, the isolated organ oxygen injection perfusion equipment mainly uses a liquid oxygen bottle as an oxygen supply device, and the oxygen bottle matched with a machine is used for oxygen filling before use. Therefore, it is desirable to provide a convenient and safe perfusion fluid oxygen supply method.
Disclosure of Invention
The invention provides an isolated organ perfusion system with an oxygen injection function, wherein an oxygen injection function unit can independently prepare oxygen in real time and supply oxygen to perfusate.
The technical scheme adopted by the invention is as follows:
an oxygen injection perfusion system for an isolated organ, comprising a perfusion circuit, an oxygenator, characterized by further comprising a molecular sieve oxygen generation unit, the molecular sieve oxygen generation unit supplying oxygen to the oxygenator.
Further, the molecular sieve oxygen production unit comprises:
the compressor is used for compressing air to form compressed air;
the double adsorption towers are used for adsorbing nitrogen in the compressed air;
and the control valve is used for controlling the flow direction of the compressed air entering the double adsorption towers, realizing that the compressed air enters one adsorption tower once and reversely cuts the other adsorption tower, and discharging the waste gas of the other adsorption tower.
Further, the adsorption tower is in an alternating working mode.
Furthermore, the molecular sieve adsorbent of the adsorption tower is 5A zeolite, and the structural formula of the 5A zeolite is 3/4 CaO.1/4 Na 2 O·Al 2 O 3 ·2SiO 2 ·9/2H 2 O, the aperture is 5A, can absorb any molecule smaller than the aperture, the 5A zeolite has high selective adsorption and high adsorption speed, is particularly suitable for pressure swing adsorption, can be suitable for pressure swing adsorption devices of gases such as oxygen production, hydrogen production, carbon dioxide production and the like with various sizes, and is a fine product in the pressure swing adsorption industry
Further, the molecular sieve oxygen production unit comprises a fine filtration tower. Oxygen from the molecular sieve tower passes through an antibacterial filter before entering the fine filtration tower, the bacterial filtration efficiency of the antibacterial filter can reach 99.9%, and the virus filtration efficiency can also reach 99.9%. The gas after passing through the antibacterial filter enters a fine filtration tower which can collect the oxygen with high purity and cleanliness prepared at the front end
Further, the molecular sieve oxygen production unit comprises an oxygen flow meter for adjusting the flow rate of the oxygen output by the molecular sieve oxygen production unit.
The invention also provides an operation method of the molecular sieve oxygen generation unit of the oxygen injection and perfusion system, which comprises the following steps:
step one, a controller controls a compressor to operate, and air is compressed by the compressor to become compressed air;
and step two, the controller controls the control valve to act, the compressed air enters the first adsorption tower to be subjected to nitrogen adsorption, one part of oxygen flowing out of the first adsorption tower is supplied to the oxygenator, the other part of oxygen flows into the second adsorption tower, and the second adsorption tower is subjected to back flushing.
Further, the method comprises the following steps: when the first adsorption tower is saturated, the controller controls the control valve to act, so that the compressed air enters the second adsorption tower to be subjected to nitrogen adsorption; and B, supplying one part of oxygen flowing out of the second adsorption tower to an oxygenator, allowing the other part of the oxygen to enter the first adsorption tower, blowing back the first adsorption tower, and restarting the step B when the second adsorption tower is saturated.
Compared with the prior art, the invention has the following advantages:
the organ mechanical perfusion system directly uses air to generate oxygen by integrating the small molecular sieve oxygen generating unit, and the generated oxygen is connected into the membrane oxygenator, so that the injection of the oxygen into perfusion fluid is realized, the continuous oxygen injection at any time can be realized, and potential safety hazards caused by oxygen tank transportation and the like are eliminated. The molecular sieve oxygen production unit can control the oxygen concentration and flow, thereby realizing various perfusion requirements of organs in different states on aerobic perfusion.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present invention.
FIG. 2 is a schematic diagram of the structure of a molecular sieve oxygen generation unit of the present invention.
Reference numerals:
organ bath-10; a perfusion pump-21; an oxygenation pump-22; a filter-30; a bubble trap-40; a bubble sensor-50; a liquid path pressure sensor-60; an oxygenator-70; molecular sieve column-801; a compressor-802; a fine filtration tower-803; control system-804; oxygen supply pump-805; a tee-806; rotary valves-FA 1, FA2, FB1, FB2; flow path-110; oxygen injection pathway-120; adsorption column-A; adsorption column-B; a central control unit-90.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, however, the embodiments described below are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Fig. 1 shows an embodiment of a cryomechanical perfusion preservation device, which mainly comprises an organ tank 10 for placing an organ to be transplanted, a perfusion pump 21 and an oxygenation pump 22, a filter 30, a bubble trap 40, a bubble sensor 50, a liquid path pressure sensor 60, an oxygenator 70, a molecular sieve tower 801, a compressor 802, a fine filtration tower 803, and a central control unit 90.
An organ, which may be a kidney ex vivo of a human or animal, is disposed within the organ bath. The organ bath is configured to allow a perfusate bath to form around the organ. The organ cassette is configured to provide uninterrupted sterile conditions during transport, recovery, analysis and storage. The organ bath is arranged in an ice box (not shown) having a recess, which can contain a cooling medium such as ice, ice water, saline, etc., although any other suitable cooling medium may be used. In use, the organ is placed in the organ bath and the organ bath is placed in the ice bank such that the ice bank surrounds the periphery of the organ bath, and the organ bath is maintained at a cryogenic environment of 0-8 ℃.
The filter is used to filter particulate matter in the fluid path and prevent the particulate matter from entering and clogging the flow path of the device. The particulate matter may be, for example, fat particles or blood clots produced by the isolated organ. The filter may be a single filter or a combination of filters having different pore sizes.
The perfusion and oxygenation pumps may be any pump suitable in connection with perfusion of an organ. Examples of suitable pumps may include manually operated pumps, centrifugal pumps, and peristaltic pumps, with commercially available peristaltic pumps being used herein.
The bubble trap preferably separates bubbles that may be entrained in the perfusate flow, preventing them from continuing downstream and into the isolated organ. The bubble catcher can also be used as a heat exchange unit for the rapid refrigeration of the perfusate. The bubble trap is generally in the shape of a cube with a lower portion and a triangle with an upper portion, and includes a circulation outlet that allows for venting during circulation or priming. The circulation outlet may be connected to the perfusate flow path. After the circulation mode is initiated, the circulation line to which the circulation outlet is connected is opened in order to purge air or other gases. Once the gas is purged from the perfusate path, the circulation outlet may be closed. The recycling outlet can be closed by a controller in the control and display unit controlling the solenoid valve.
The perfusion circuit comprises a flow path 110, the inlet of the perfusate of the organ tank 10 is connected with one end of a perfusion pump 21 through a pipeline, the other end of the perfusion pump is connected with the liquid inlet of a filter 30 through a pipeline, the liquid outlet of the filter 30 is connected with the liquid inlet of a bubble catcher 40 through a pipeline, the liquid of the bubble catcher 40 flows into the artery of the isolated organ, flows out from the vein and forms circulation to form a perfusion circuit, and the low-temperature mechanical continuous perfusion of the isolated organ is realized. The tubing may be a suitable flexible fluid conduit.
A bubble sensor 50 is also provided around the downstream perfusion line of the bubble trap to detect whether bubbles remain in the perfusate. If the presence of air bubbles is detected, the perfusion is stopped. The bubble sensor may be an ultrasonic bubble sensor that may not contact the perfusate, and therefore need not be cleaned and is easily replaced.
The filling line is provided with a liquid path pressure sensor 60. The fluid circuit pressure sensor detects the real-time pressure of the fluid circuit and provides overpressure monitoring. In the event that the perfusate pressure flowing through the tubing exceeds a predetermined threshold, the device can automatically stop and/or reduce the flow provided by the pump to prevent damage to the organ.
The device also includes an oxygen injection pathway 120. The perfusate in the organ tank 10 enters the oxygenator 70 through the oxygenating pump 22, is oxygenated with oxygen from the molecular sieve column 801 and further processed by the fine filtering column 803, and the oxygenated perfusate enters the flow path 110.
The membrane oxygenator adopts a hollow fiber membrane, supplies oxygen to the perfusate and removes carbon dioxide in the liquid, and needs to be provided in a sterile and pyrogen-free state.
Fig. 2 shows the structural composition of the oxygen supply unit of the present invention.
The molecular sieve oxygen production unit comprises a molecular sieve tower 801, a compressor 802, a fine filtration tower 803, a control system 804, an oxygen supply pump 805, rotary valves FA1, FA2, FB1, FB2, a tee 806 and a flow meter, wherein the molecular sieve tower 801 comprises an adsorption tower A and an adsorption tower B.
The working principle of the adsorption tower is as follows: the characteristic that nitrogen molecules are larger than oxygen molecules is utilized, and a special molecular sieve is utilized to separate oxygen in the air. Firstly, dry air is forced to pass through the molecular sieve by a compressor and enter an absorption tower which is vacuumized, nitrogen molecules in the air are absorbed by the molecular sieve, and oxygen with higher purity is obtained. After a period of time, the nitrogen adsorbed by the molecular sieve is gradually increased, the adsorption capacity is weakened, the purity of the produced oxygen is reduced, the controller controls the rotary valve to switch to enable the compressed air to enter another molecular sieve tower, meanwhile, the pressure release port of the previous molecular sieve tower is opened, the molecular sieve can desorb the nitrogen after the pressure is reduced, waste gases such as the nitrogen are removed by the oxygen reversely pushed in the other molecular sieve, and then the process is repeated.
The adsorption tower takes a zeolite molecular sieve as an adsorbent, and preferentially adsorbs harmful gases such as carbon dioxide, sulfide, nitrogen and the like in the air by utilizing the characteristic that the zeolite molecular sieve filled with micropores has different adsorption capacities on oxygen and nitrogen in the air, so that high-purity oxygen which meets medical standards and is suitable for being carried by a perfusion system is obtained. The molecular sieve stone is sealed in the sieve body in a physical packaging mode after being filled, and the filling amount is generally controlled to be 90% of the cavity of the sieve body.
In this example, the molecular sieve oxygen generation unit works as follows.
Air enters a compressor 802 through a filter, compressed air enters an adsorption tower A or an adsorption tower B through a rotary valve for adsorption separation, and 5A zeolite is filled in the adsorption tower A and the adsorption tower B. The control system 804 controls the rotary valve to change the adsorption period and to distribute the intake and exhaust flow directions. Taking one cycle in the process as an example, the rotary valves FA1 and FA2 are opened, the FB1 and FB2 are closed, the compressed air enters the adsorption tower a, at this time, nitrogen in the compressed air is adsorbed into the molecular sieve in the adsorption tower a, oxygen flows out through the tee 806 at the top end of the adsorption tower a, and a part of the oxygen flows out from the port b, passes through the fine filtration tower 803 and the flow meter to be output, and enters the oxygenator; one part of the nitrogen-rich desorption gas flows out from the port c and is used for back flushing the adsorption tower B in a desorption state, and the nitrogen-rich desorption gas is discharged as waste gas. When the molecular sieve in the adsorption tower A reaches a theoretical adsorption saturation critical state, the control system 804 controls to close the rotary valves FA1 and FA2, open the rotary valves FB1 and FB2, switch the inlet air to the adsorption tower B, and simultaneously perform pressure reduction and desorption on the adsorption tower A. The working process of the adsorption tower B is completely the same as that of the adsorption tower A, and the adsorption tower B and the adsorption tower A work alternately to provide continuous oxygen supply.
The molecular sieve oxygen production unit of the device is provided with a flowmeter, can provide flow output with the oxygen flow range of 1L/min-5L/min, and provides the oxygen concentration meeting the requirement of 93 percent of the concentration specified by 5.2 in YY/T0298-1998. The size of molecular sieve system oxygen unit is 40 × 15cm, accessible fixed bolster embedded with the low temperature perfusion apparatus inside, can not influence the appearance of low temperature perfusion apparatus.
Claims (8)
1. An oxygen injection perfusion system for an isolated organ, comprising a perfusion circuit, an oxygenator, characterized by further comprising a molecular sieve oxygen generation unit, the molecular sieve oxygen generation unit supplying oxygen to the oxygenator.
2. The oxygen perfusion system of claim 1, wherein the molecular sieve oxygen generation unit comprises:
the compressor is used for compressing air to form compressed air;
the double adsorption towers are used for adsorbing nitrogen in the compressed air;
and the control valve is used for controlling the flow direction of the compressed air entering the double adsorption towers, so that the compressed air enters one adsorption tower at a time and reversely blows the other adsorption tower.
3. The oxygen perfusion system of claim 2, wherein the adsorption tower is in an alternating mode of operation.
4. The oxygen injection perfusion system of claim 3, wherein the adsorbent of the adsorption column is a 5A zeolite.
5. The oxygen perfusion system of claim 3, further comprising a polishing tower.
6. The oxygen injection and perfusion system according to claim 3, further comprising a flow meter for regulating the flow rate of oxygen output by the molecular sieve oxygen production unit.
7. Method of operating a molecular sieve oxygen generation unit of an oxygen perfusion system according to any of the claims from 3 to 6, comprising the following steps:
step one, a controller controls a compressor to operate, and air is compressed by the compressor to become compressed air;
and step two, the controller controls the control valve to act, the compressed air enters the first adsorption tower to be subjected to nitrogen adsorption, one part of oxygen flowing out of the first adsorption tower is supplied to the oxygenator, the other part of oxygen flows into the second adsorption tower, and the second adsorption tower is subjected to back flushing.
8. The method of operating a molecular sieve oxygen generation unit of an oxygen injection perfusion system of claim 7, further comprising the steps of three: when the first adsorption tower is saturated, the controller controls the control valve to act, so that the compressed air enters the second adsorption tower to be subjected to nitrogen adsorption; and B, supplying one part of oxygen flowing out of the second adsorption tower to an oxygenator, allowing the other part of the oxygen to enter the first adsorption tower, blowing back the first adsorption tower, and restarting the step B when the second adsorption tower is saturated.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211041013.4A CN115428785A (en) | 2022-08-29 | 2022-08-29 | Oxygen injection perfusion system for isolated organ and operation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211041013.4A CN115428785A (en) | 2022-08-29 | 2022-08-29 | Oxygen injection perfusion system for isolated organ and operation method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1522788A (en) * | 2003-09-12 | 2004-08-25 | 北京科技大学 | Pressure swing molecular screen adsorption oxygen making device |
| CN2643985Y (en) * | 2003-09-28 | 2004-09-29 | 北京科技大学 | Transformation and absorption device for high concentration oxygen by use of air separation |
| CN103143334A (en) * | 2013-03-06 | 2013-06-12 | 富阳凯合空分设备有限公司 | Molecular sieve activation device for pressure swing adsorption oxygen preparation device |
| CN104797133A (en) * | 2012-07-10 | 2015-07-22 | 生命线科学有限公司 | Organ transporter with oxygen generation |
| CN211733852U (en) * | 2019-09-29 | 2020-10-23 | 福州市欣鑫磊机械设备有限责任公司 | Pressure swing adsorption oxygen generating device |
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- 2022-08-29 CN CN202211041013.4A patent/CN115428785A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1522788A (en) * | 2003-09-12 | 2004-08-25 | 北京科技大学 | Pressure swing molecular screen adsorption oxygen making device |
| CN2643985Y (en) * | 2003-09-28 | 2004-09-29 | 北京科技大学 | Transformation and absorption device for high concentration oxygen by use of air separation |
| CN104797133A (en) * | 2012-07-10 | 2015-07-22 | 生命线科学有限公司 | Organ transporter with oxygen generation |
| CN103143334A (en) * | 2013-03-06 | 2013-06-12 | 富阳凯合空分设备有限公司 | Molecular sieve activation device for pressure swing adsorption oxygen preparation device |
| CN211733852U (en) * | 2019-09-29 | 2020-10-23 | 福州市欣鑫磊机械设备有限责任公司 | Pressure swing adsorption oxygen generating device |
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