CN110786322A - Isolated lung storage and perfusion device - Google Patents
Isolated lung storage and perfusion device Download PDFInfo
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- CN110786322A CN110786322A CN201911248871.4A CN201911248871A CN110786322A CN 110786322 A CN110786322 A CN 110786322A CN 201911248871 A CN201911248871 A CN 201911248871A CN 110786322 A CN110786322 A CN 110786322A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0247—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
Abstract
The invention relates to an isolated lung storage and perfusion device, which comprises a storage and perfusion box body; a perfusion circulation mechanism and a breathing circulation mechanism are arranged in the storage perfusion box body, and the perfusion circulation mechanism and the breathing circulation mechanism are electrically connected with a perfusion breathing controller in the storage perfusion box body; after the perfusion circulation mechanism is in adaptive connection with pulmonary artery and pulmonary vein of the isolated lung, the perfusion breathing controller controls the perfusion circulation mechanism to perform continuous perfusion on the connected isolated lung, and metabolites generated by the isolated lung can be removed in the perfusion process of the perfusion; the perfusion breathing controller controls the breathing circulation mechanism to assist the in-vitro lung to breathe, so that the in-vitro lung can keep a normal breathing state. The invention can effectively realize the cleaning and perfusion of the isolated lung in the transportation process, effectively remove the metabolite produced in the transportation process of the isolated lung, provide the respiratory circulation of the isolated lung in the transportation process, ensure the function of the isolated lung in transportation, and is safe and reliable.
Description
Technical Field
The invention relates to a storage and perfusion device, in particular to an isolated lung storage and perfusion device, and belongs to the technical field of isolated lung storage.
Background
In the lung transplantation operation, the isolated lung needs to be taken out first. After the isolated lung is taken out, the isolated lung is placed in an isolated lung transfer box after being perfused by perfusate, and then the isolated lung transfer box and the isolated lung are transported to a destination. Generally, the transport time of the isolated lung can reach 6-7 hours or even longer, but in the transport process of the isolated lung, the metabolic products of the isolated lung cannot be discharged in the isolated lung due to the fact that perfusion operation cannot be carried out in the transport box. After transplantation of the ex vivo lung, the metabolites in the ex vivo lung follow the body's circulation into the recipient's blood, resulting in an even more severe response by the recipient of the ex vivo lung.
In addition, the separation lung is in a stable state under the low-temperature environment of the transfer box, so that the separation lung cannot keep a breathing state under a normal condition, great damage can be brought to the separation lung, the function of the separation lung is reduced, and the failure of lung transplantation operation can be seriously caused.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides an isolated lung storage and perfusion device which can effectively realize the cleaning and perfusion of the isolated lung in the transportation process, effectively remove metabolites generated in the transportation process of the isolated lung, provide the respiratory circulation of the isolated lung in the transportation process, ensure the function of the isolated lung in transportation, and is safe and reliable.
According to the technical scheme provided by the invention, the isolated lung storage and perfusion device comprises a storage and perfusion box body which can be used for storing and transporting the isolated lung; the storage perfusion box is internally provided with a perfusion circulation mechanism which can be in adaptive connection with the isolated lung and a respiration circulation mechanism which can be in adaptive connection with the isolated lung, the perfusion circulation mechanism and the respiration circulation mechanism are both electrically connected with a perfusion respiration controller in the storage perfusion box, and the corresponding working states of the perfusion circulation mechanism and the respiration circulation mechanism can be controlled by the perfusion respiration controller;
after the perfusion circulation mechanism is in adaptive connection with pulmonary artery and pulmonary vein of the isolated lung, the perfusion breathing controller controls the perfusion circulation mechanism to perform continuous perfusion on the connected isolated lung, and metabolites generated by the isolated lung can be removed in the perfusion process of the perfusion; the perfusion breathing controller controls the breathing circulation mechanism to assist the in-vitro lung to breathe, so that the in-vitro lung can keep a normal breathing state.
The storage perfusion box is internally provided with a heat insulation layer, and the storage perfusion box is internally further provided with a storage and transportation refrigeration source which can provide low-temperature environment required by in-vitro lung storage and transportation.
Store and transport refrigeration source including placing in the ice-cube of storing the filling box or can realize the refrigerated semiconductor refrigerator of work, semiconductor refrigerator and control semiconductor refrigerator operating condition's the pouring of breathing controller is connected, it breathes the temperature that the controller passes through temperature sensor can gather in real time to store in the filling box to fill, fills and breathes the temperature environment that the controller passes through temperature sensor and semiconductor refrigerator and can make to store in the filling box to reach the separation lung and transport.
The perfusion circulating mechanism comprises a perfusion injector capable of containing perfusion liquid, a perfusion driver matched with the perfusion injector, and a pulmonary vein first connecting pipe, a pulmonary vein second connecting pipe, a pulmonary artery first connecting pipe and a pulmonary artery second connecting pipe which can be communicated with the perfusion injector;
through the first connecting pipe of pulmonary vein, pulmonary vein second connecting pipe can with pulmonary vein adaptation connection, through the first connecting pipe of pulmonary artery, pulmonary artery second connecting pipe can with pulmonary artery adaptation connection, perfusate in the drive perfusion liquid ware can be through the first connecting pipe of pulmonary artery, pulmonary artery second connecting pipe gets into the pulmonary artery of separation lung, and the perfusate that gets into in the separation lung can be through the first connecting pipe of pulmonary vein, pulmonary vein second connecting pipe returns once more to in the perfusion liquid ware, in order to realize carrying out the perfusate perfusion circulation that lasts to the separation lung.
The pulmonary artery perfusion device is characterized by further comprising a perfusion liquid outlet pipe and a perfusion liquid inlet pipe which can be in adaptive connection with the perfusion liquid device, wherein one end of the perfusion liquid inlet pipe is connected with the perfusion liquid device, the other end of the perfusion liquid inlet pipe is connected and communicated with the pulmonary vein first connecting pipe and the pulmonary vein second connecting pipe, one end of the perfusion liquid outlet pipe is connected with the perfusion liquid device, and the other end of the perfusion liquid outlet pipe is connected and communicated with the pulmonary artery first connecting pipe and the pulmonary artery second connecting pipe;
set up the filtration remover that is used for getting rid of metabolite in the separation lung on pouring into the drain pipe, set up on pouring into the feed liquor pipe and be used for the gaseous exhalant gas separator in the perfusate.
A liquid outlet one-way valve is arranged at the end part of the perfusion liquid outlet pipe adjacent to the perfusion injector, and a liquid inlet one-way valve is arranged at the end part of the perfusion liquid inlet pipe adjacent to the perfusion injector;
the perfusate ware including can accept the perfusate receptacle of perfusate and set up in the drive elastic ring that fills on upper portion in the perfusate receptacle, fill drive elastic ring and perfusate receptacle adaptation and be connected, can drive through the perfusion driver and fill drive elastic ring reciprocating motion in the perfusate receptacle, fill when drive elastic ring reciprocating motion in the perfusate receptacle, can make the perfusate in the perfusate feed liquor pipe get into the perfusate of perfusate receptacle lower part and accept the intracavity, and can make the perfusate that the perfusate accepts the intracavity get into in the perfusate goes out the liquid pipe.
The gas separator comprises a plurality of exhaust holes arranged on the filling liquid inlet pipe and a water-blocking and breathable film arranged in the exhaust holes, and the filtering remover comprises an adsorption filtering film body capable of adsorbing and separating metabolites.
The first connecting pipe of the pulmonary vein is provided with a first connecting pipe clamp, the second connecting pipe of the pulmonary vein is provided with a second connecting pipe clamp, the first connecting pipe of the pulmonary artery is provided with a third connecting pipe clamp, and the second connecting pipe of the pulmonary artery is provided with a fourth connecting pipe clamp.
Breathe circulation mechanism including can extract the gas generator of storing gas in the filling box, can drive gas generator's extraction driver and can carry out filterable gas filter to the gas that gas generator extracted, gas filter's gas outlet sets up the trachea that can with the trachea adaptation of separation lung and puts the pipe, the extraction driver is connected with the perfusion breathing controller electricity, and the perfusion breathing controller can control the extraction driver and drive gas generator and get into required operating condition.
The gas generator comprises a generator shell, a gas extraction elastic ring arranged in the generator shell and a filtering gas inlet arranged at the bottom of the generator shell; the extraction driver can drive the gas extraction elastic ring to reciprocate in the generator shell, and when the gas extraction elastic ring reciprocates in the generator shell, gas in the storage filling box body can enter the generator shell through the filtering air inlet, and the gas in the generator shell can enter the trachea of the isolated lung through the gas filter and the trachea cannula.
The invention has the advantages that: the storage and the transportation to the separation lung can be realized through storing the perfusion box body, can realize the continuous perfusion of separation lung in storage and transportation process through storing the perfusion circulation mechanism in the perfusion box body, can realize storing and transportation in the separation lung through breathing circulation mechanism and supporting, can effectively realize the washing of pouring into of separation lung in the transportation process, effectively clear away the metabolite that produces in the separation lung transportation process, provide the breathing circulation of separation lung in the transportation process, ensure the function that the separation lung transported, safety and reliability.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural diagram of the first box cover of the present invention.
Fig. 3 is a schematic structural view of a second case cover according to the present invention.
FIG. 4 is a schematic view of the perfusion cycle mechanism of the present invention.
FIG. 5 is a schematic diagram of an embodiment of the perfusion circulation mechanism of the present invention.
FIG. 6 is a schematic diagram of a breathing cycle mechanism of the present invention.
Description of reference numerals: 1-storage perfusion box body, 2-heat insulation layer, 3-box body pull ring, 4-box body power supply, 5-perfusion circulation mechanism, 6-respiration circulation mechanism, 7-first box body cover, 8-perfusion circulation placing area, 9-perfusion elastic ring driving body, 10-perfusion elastic ring driving plate, 11-second box body cover, 12-respiration circulation placing area, 13-extraction elastic ring driving body, 14-extraction elastic ring driving plate, 15-pulmonary vein first connecting pipe, 16-pulmonary vein second connecting pipe, 17-first connecting pipe clamp, 18-second connecting pipe clamp, 19-perfusion liquid inlet pipe, 20-gas separation container, 21-water-blocking and gas-permeable film, 22-liquid inlet one-way valve, 23-perfusion device, 24-liquid outlet one-way valve, 25-filtering remover, 26-perfusion liquid outlet pipe, 27-third connecting pipe clamp, 28-fourth connecting pipe clamp, 29-pulmonary artery first connecting pipe, 30-pulmonary artery second connecting pipe, 31-liquid injection port, 32-perfusate container, 33-perfusion driving elastic ring, 34-perfusate containing cavity, 35-filtering removal container, 36-adsorption filtering membrane body, 37-generator shell, 38-gas extraction elastic ring, 39-filtering air inlet, 40-gas filter, 41-trachea placing pipe, 42-trachea placing end and 43-exhaust valve.
Detailed Description
The invention is further illustrated by the following specific figures and examples.
As shown in fig. 1, 2 and 3: in order to realize the perfusion cleaning of the isolated lung in the transfer process, effectively remove metabolites generated in the transfer process of the isolated lung, provide the respiratory circulation of the isolated lung in the transfer process and ensure the function of the isolated lung in transfer, the invention comprises a storage perfusion box body 1 which can be used for storing and transferring the isolated lung; a perfusion circulation mechanism 5 which can be in adaptive connection with the isolated lung and a breathing circulation mechanism 6 which can be in adaptive connection with the isolated lung are arranged in the storage perfusion box body 1, the perfusion circulation mechanism 5 and the breathing circulation mechanism 6 are both electrically connected with a perfusion breathing controller in the storage perfusion box body 1, and the corresponding working states of the perfusion circulation mechanism 5 and the breathing circulation mechanism 6 can be controlled through the perfusion breathing controller;
after the perfusion circulation mechanism 5 is in adaptive connection with the pulmonary artery and the pulmonary vein of the isolated lung, the perfusion breathing controller controls the perfusion circulation mechanism 5 to perform continuous perfusion on the connected isolated lung, and metabolites generated by the isolated lung can be removed in the perfusion process of the perfusion; the perfusion breathing controller controls the breathing circulation mechanism 6 to assist the in-vitro lung to breathe, so that the in-vitro lung can keep a normal breathing state.
Specifically, the storage and filling box 1 is made of a material meeting medical standards, and the specific type of the material can be selected according to needs, which is well known to those skilled in the art and will not be described herein again. The storage and the reuse of the isolated lung can be realized through the storage perfusion box body 1. During concrete implementation, set up in the storage perfusion box 1 and fill circulation mechanism 5, breathing circulation mechanism 6 and fill respiratory controller, can be opposite to placing through filling circulation mechanism 5, breathing circulation mechanism 6 store the separation lung in the attention box 1 and connect the cooperation, can be connected with perfusion circulation mechanism 5, breathing circulation mechanism 6 electricity through filling respiratory controller, fills respiratory controller promptly and can control the corresponding operating condition of perfusion circulation mechanism 5, breathing circulation mechanism 6.
During specific implementation, the perfusion circulation mechanism 5 can be in adaptive connection with pulmonary artery and pulmonary vein of the isolated lung, continuous perfusion of the connected isolated lung can be realized through the perfusion circulation mechanism 5, and in the perfusion process, metabolic products generated by the isolated lung can be removed, and meanwhile, gas in the perfusion liquid can be separated and discharged, so that the reaction of the isolated lung after transplantation can be effectively reduced, and the reliability of the isolated lung after transplantation is ensured. The respiration of the isolated lung can be assisted through the respiration circulation mechanism 6, the respiration state of the isolated lung in the transportation process is kept, and the function of the isolated lung in the transportation process is ensured.
Specifically, the perfusion breathing controller can adopt the existing common micro-processing mode, such as adopting a single chip microcomputer and the like, and can be specifically selected according to the needs, which is not described herein again. Generally, in order to adapt to the storage of the isolated lung, the storage perfusion box 1 comprises a first box cover 7 and a second box cover 11 matched with the first box cover 7, and when the first box cover 7 and the second box cover 11 are close to each other and matched, the storage perfusion box 1 can be formed, so that the isolated lung can be stored in a closed manner; when the first box cover 7 and the second box cover 11 are separated from each other, the separated lung can be placed in the storage perfusion box 1. The box body pull ring 3 is arranged on the first box body cover 7 and/or the second box body cover 11, and the storage filling box body 1 can be conveniently held and stored through the box body pull ring 3.
In the embodiment of the invention, the perfusion circulation mechanism 5 is arranged in the perfusion circulation placing area 8 in the first box cover 7, the breathing circulation mechanism 6 is arranged in the breathing circulation placing area 12 in the second box cover 11, and a cavity adaptive to the isolated lung can be formed between the first box cover 7 and the second box cover 11, so that when the isolated lung is arranged between the first box cover 7 and the second box cover 11, the perfusion circulation mechanism 5 and the breathing circulation mechanism 6 can be adaptively connected with the isolated lung, and continuous perfusion and auxiliary breathing support of the isolated lung are realized. The box power supply 4 which can provide the work requirement of the perfusion breathing controller is required to be arranged in the storage perfusion box 1, the box power supply 4 can adopt a lithium battery or other power supply forms, the box power supply 4 can adopt a rechargeable or replaceable form, and a common connection form can be adopted between the box power supply 4 and the perfusion breathing controller, which is particularly well known by the people in the technical field and is not described again.
Further, set up insulating layer 2 in storing and filling box 1 store and fill and still set up the storage that can provide the required low temperature environment of separation lung storage and transportation in box 1 and transport the refrigeration source. In the embodiment of the invention, the temperature condition required by the isolated lung in the storage and perfusion box body 1 can be provided by the storage, transportation and refrigeration source, so that the safety and reliability of the isolated lung in the storage and perfusion box body 1 are ensured. Can reduce to store through insulating layer 2 and transport the refrigeration source in external heat exchange, ensure to store and fill the stability of box 1 internal temperature, insulating layer 2 in first box lid 7, second box lid 11, insulating layer 2 can adopt current commonly used form, as long as can realize thermal-insulated purpose all can, and concrete condition is no longer repeated.
Store and transport refrigeration source including placing in storing the ice-cube that fills in box 1 or can realize the refrigerated semiconductor refrigerator of work, semiconductor refrigerator and control semiconductor refrigerator operating condition's the perfusion breathing controller connection, perfusion breathing controller passes through temperature sensor can gather in real time and store the temperature that fills in box 1, perfuses breathing controller and can make the temperature environment that reaches the separation lung transportation in the storage fills box 1 through temperature sensor and semiconductor refrigerator.
In the embodiment of the invention, when the ice blocks are arranged in the storage and perfusion box body 1, the temperature conditions in the storage and perfusion box body 1 can meet the temperature conditions required by the storage and transportation of the in vitro lung through the low temperature of the ice blocks, and the refrigeration mode of the ice blocks is consistent with the refrigeration mode in the existing storage and transportation process of the in vitro lung. Of course, in the specific implementation, a semiconductor refrigerator can be adopted, the semiconductor refrigerator can adopt the existing common form, the semiconductor refrigerator is electrically connected with the perfusion breathing controller, and the semiconductor refrigerator can keep the temperature condition in the storage perfusion box body 1 at-5 ℃ to 10 ℃ when working. Of course, other refrigeration forms can be adopted in the storage perfusion box body 1, as long as the temperature environment of the isolated lung during the storage and transportation processes in the storage perfusion box body 1 can be met, and the method is not listed here.
As shown in fig. 4 and 5, the perfusion circulation mechanism 5 includes a perfusion unit 23 capable of containing a perfusion fluid, a perfusion driver adapted to the perfusion unit 23, and a pulmonary vein first connecting tube 15, a pulmonary vein second connecting tube 16, a pulmonary artery first connecting tube 29, and a pulmonary artery second connecting tube 30 capable of communicating with the perfusion unit 23;
through the first connecting tube of pulmonary vein 15, the pulmonary vein second connecting tube 16 can with pulmonary vein adaptation connection, through the first connecting tube of pulmonary artery 29, pulmonary artery second connecting tube 30 can with pulmonary artery adaptation connection, perfusate in the driver can drive perfusion injector 23 gets into the pulmonary artery of separation lung through the first connecting tube of pulmonary artery 29, pulmonary artery second connecting tube 30, and the perfusate that gets into in the separation lung can be through the first connecting tube of pulmonary vein 15, pulmonary vein second connecting tube 16 returns once more to in perfusion injector 23, in order to realize carrying out lasting perfusate perfusion circulation to the separation lung.
In the embodiment of the present invention, the perfusion injector 23 can accommodate the perfusion fluid, the perfusion fluid can be placed in the perfusion injector 23 in a pre-filling or perfusion manner, the perfusion fluid is a fluid that can currently perfuse the isolated lung, and the specific form of the perfusion fluid is well known to those skilled in the art, and will not be described herein again. The perfusion driver is matched with the perfusion device 23, and can provide power for circulation of the perfusion liquid in the perfusion device 23 through the perfusion driver, so that perfusion circulation of the perfusion liquid is realized. In order to be matched with the isolated lung, the first pulmonary vein connecting pipe 15 and the second pulmonary vein connecting pipe 16 can be connected with the pulmonary vein of the isolated lung, and the first pulmonary artery connecting pipe 29 and the second pulmonary artery connecting pipe 30 can be matched and connected with the pulmonary artery of the isolated lung. After being connected with separation lung adaptation and through perfusion driver drive perfusion liquid ware 23, the perfusate in the perfusate ware 23 can be via the pulmonary artery first connecting pipe 29, the pulmonary artery second connecting pipe 30 gets into the pulmonary artery of separation lung, the perfusate that gets into in the separation lung can be via the pulmonary vein first connecting pipe 15, the pulmonary vein second connecting pipe 16 returns to in the perfusion liquid ware 23 once more, along with perfusion driver and perfusion liquid ware 23 continuously cooperate, can continuously realize the cyclic motion of perfusate, thereby realize carrying out the perfusion liquid circulation that lasts to the separation lung.
Further, the device also comprises a perfusion liquid outlet pipe 26 and a perfusion liquid inlet pipe 19 which can be in adaptive connection with the perfusion liquid device 23, wherein one end of the perfusion liquid inlet pipe 19 is connected with the perfusion liquid device 23, the other end of the perfusion liquid inlet pipe 19 is connected and communicated with the pulmonary vein first connecting pipe 15 and the pulmonary vein second connecting pipe 16, one end of the perfusion liquid outlet pipe 26 is connected with the perfusion liquid device 23, and the other end of the perfusion liquid outlet pipe 26 is connected and communicated with the pulmonary artery first connecting pipe 29 and the pulmonary artery second connecting pipe 30;
a filtration remover 25 for removing the metabolites in the isolated lung is arranged on the perfusion liquid outlet pipe 26, and a gas separator for discharging the gas in the perfusion liquid is arranged on the perfusion liquid inlet pipe 19.
In the embodiment of the invention, the pipe diameter of the perfusion liquid inlet pipe 19 is larger than the pipe diameters of the pulmonary vein first connecting pipe 15 and the pulmonary vein second connecting pipe 16, the length directions of the pulmonary vein first connecting pipe 15 and the pulmonary vein second connecting pipe 16 are consistent with the length direction of the perfusion liquid inlet pipe 19, and the pulmonary vein first connecting pipe 15 and the pulmonary vein second connecting pipe 16 can be communicated with the perfusion liquid container 23 through the perfusion liquid inlet pipe 19. Similarly, the diameter of the perfusion outlet pipe 26 is larger than the diameters of the first pulmonary artery connecting pipe 29 and the second pulmonary artery connecting pipe 30, and the diameters of the first pulmonary artery connecting pipe 29 and the second pulmonary artery connecting pipe 30 can be communicated with the perfusion unit 23 through the perfusion outlet pipe 26.
A filtration remover 25 is provided on the perfusion outlet pipe 26, and a gas separator is provided on the perfusion inlet pipe 19. The gas separator comprises a plurality of exhaust holes arranged on the perfusion liquid inlet pipe 19 and a water-blocking and gas-permeable membrane 21 arranged in the exhaust holes, and the filtering remover 25 comprises an adsorption filtering membrane body 36 capable of adsorbing and separating metabolites. Specifically, the gas separator includes the gas separation container 20 that can communicate with filling feed liquor pipe 19, set up a plurality of exhaust holes on gas separation container 20, all set up the ventilated membrane 21 that blocks water in every exhaust hole, the perfusate that gets into in filling liquid ware 23 through tubulose feed liquor pipe 19 gets into in gas separation container 20 earlier, when there is gas in the perfusate, the gas in the perfusate can be discharged through ventilated membrane 20 that blocks water, and can prevent the perfusate to follow gas through ventilated membrane 20 that blocks water and discharge from the exhaust hole, after gaseous the discharge, the perfusate reenters liquid ware 23 in, along with the continuation of pouring, can effectively discharge gas, avoid the gas existence in the perfusate and lead to the problem of air embolism. Generally, a liquid injection port 31 may be further provided at the bottom of the gas separation container 20, and a desired chemical liquid, such as physiological saline, a perfusion liquid, etc., can be injected into the gas separation container 20 through the liquid injection port 31, and the injected liquid can be mixed with the perfusion liquid in the gas separation container 20 and follow a perfusion cycle of the perfusion liquid.
In the embodiment of the present invention, the filtering remover 25 further includes a filtering removal container 35, the adsorption and filtration membrane 36 is disposed in the filtering removal container 35, the perfusate entering the pulmonary artery from the perfusion injector 23 first enters the filtering removal container 35, the perfusate is in full contact with the adsorption and filtration membrane 36 in the filtering removal container 35, then enters the pulmonary artery through the pulmonary artery first connecting tube 29 and the pulmonary artery second connecting tube 30, the metabolite produced by the isolated lung can be adsorbed and removed by the adsorption and filtration membrane 36, the adsorption and filtration membrane 36 can be horizontally and vertically disposed in the filtering removal container 35, at this time, the perfusate in the perfusion injector 23 first enters the lower portion of the adsorption and filtration membrane 36, and the perfusate flows out of the filtering removal container 35 through the upper portion of the filtering removal container 35 after passing through the adsorption and filtration membrane 36; of course, the adsorption filtration membrane 36 may be distributed in other ways as long as the perfusion fluid is fully contacted. The adsorption filtration membrane 36 can adopt a matching form of a conventional common filter net membrane and a medical adsorbent, the medical adsorbent can adopt medical carbon powder and the like, the adsorption filtration membrane 36 can also adopt other structural forms, and the adsorption filtration membrane can adsorb and filter metabolites generated in the process of in vitro lung perfusion, and is not specifically described in detail.
Further, a liquid outlet one-way valve 24 is arranged at the end part of the perfusion liquid outlet pipe 26 adjacent to the perfusion injector 23, and a liquid inlet one-way valve 22 is arranged at the end part of the perfusion liquid inlet pipe 19 adjacent to the perfusion injector 23;
pouring liquid ware 23 including can accept the perfusate container 32 of perfusate and set up in the drive elastic ring 33 of pouring of upper portion in the perfusate container 32, drive elastic ring 33 and the perfusate container 32 adaptation are connected in perfusion, can drive through the perfusion driver and fill drive elastic ring 33 and reciprocating motion in the perfusate container 32, when filling drive elastic ring 33 in the perfusate container 32 reciprocating motion, can make in the perfusate entering perfusate of perfusate feed liquor pipe 19 accepts the chamber 34 of perfusate container 32 lower part, and can make in the perfusate entering of perfusate accepting the chamber 34 pours into drain pipe 26.
In the embodiment of the invention, the liquid outlet one-way valve 24 and the liquid inlet one-way valve 22 can adopt the existing common form, the one-way flow of the perfusate can be realized through the liquid inlet one-way valve 22 and the liquid outlet one-way valve 24, the liquid inlet one-way valve 22 is positioned between the gas separation container 20 and the perfusion injector 23, the liquid outlet one-way valve 24 is positioned between the perfusion injector 23 and the filtering removal container 35, and the reliability of the flow of the perfusate in the perfusion process can be ensured through the liquid inlet one-way valve 22 and the liquid outlet one-way valve 24.
The perfusion injector 23 can contain perfusion fluid through the perfusion fluid container 32, the perfusion driving elastic ring 33 is located at the upper part in the perfusion fluid container 32, the perfusion driving elastic ring 33 can be made of elastic components such as springs, the length direction of the perfusion driving elastic ring 33 is consistent with the length direction of the perfusion fluid container 32, the upper part of the perfusion fluid container 32 can reciprocate along with the perfusion driving elastic ring 33, namely the upper part of the perfusion fluid container 32 can be made of an elastic film, so that the perfusion driving elastic ring 33 can be contained in the perfusion fluid container 32, the reciprocating motion of the perfusion driving elastic ring 33 in the perfusion fluid container 32 can be ensured, and the reciprocating motion direction of the perfusion driving elastic ring 33 is the length direction of the perfusion driving elastic ring 33. When the filling driving elastic ring 33 is compressed, negative pressure can be generated in the filling liquid container 32, so that the filling liquid in the filling liquid inlet pipe 19 can enter the filling liquid containing cavity 34 at the lower part of the filling liquid container 32 through the liquid inlet one-way valve 22, and when the filling driving elastic ring 33 is reset, the filling liquid in the filling liquid containing cavity 34 can enter the filling liquid outlet pipe 26 through the liquid outlet one-way valve 24.
When the pouring injector 23 adopts the structure, the pouring driver can drive the pouring driving elastic ring 33 to reciprocate; during specific implementation, the perfusion driver comprises a perfusion elastic ring driving body 9 and a perfusion elastic ring driving plate 10, the perfusion elastic ring driving body 9 is installed on the first box cover 7, the perfusion elastic ring driving plate 10 is located right above the perfusion driving elastic ring 33, the perfusion elastic driving body 9 can adopt a linear motor, the perfusion elastic ring driving plate 10 is in adaptive connection with the perfusion elastic driving body 9, and the perfusion elastic driving body 9 can drive the perfusion driving elastic ring 33 to reciprocate through the perfusion elastic ring driving plate 10. Of course, the perfusion elastic driving body 9 can also adopt a matching form of a motor and a screw nut, and the like, as long as the perfusion elastic ring driving plate 10 can drive the perfusion driving elastic ring 33 to reciprocate, and the perfusion driving elastic ring can be specifically selected as required, and is not described herein again. The perfusion elastic driving body 9 is electrically connected with a perfusion breathing controller, and the perfusion breathing controller can control the working state of the perfusion elastic driving body 9, so that the driving control of the reciprocating motion of the perfusion driving elastic ring 33 can be realized.
Furthermore, a first connecting tube clamp 17 is arranged on the first pulmonary vein connecting tube 15, a second connecting tube clamp 18 is arranged on the second pulmonary vein connecting tube 16, a third connecting tube clamp 27 is arranged on the first pulmonary artery connecting tube 29, and a fourth connecting tube clamp 28 is arranged on the second pulmonary artery connecting tube 30.
In the embodiment of the present invention, the first connecting pipe clamp 17 can control the state of the perfusate flowing through the first pulmonary vein connecting pipe 15, the first connecting pipe clamp 17 may be an existing commonly used medical clamp, and may be specifically selected according to the need, which is not described herein again. Similarly, the second connecting pipe clamp 18, the third connecting pipe clamp 27 and the fourth connecting pipe clamp 28 have the same functions as the first connecting pipe clamp 17, and reference may be made to the description of the first connecting pipe clamp 17.
Further, breathe circulation mechanism 6 including can extract store fill the box 1 internal gas generator, can drive gas generator's extraction driver and can carry out filterable gas filter 40 to the gas of gas generator extraction, gas filter 40's gas outlet sets up can with the trachea of separation lung put pipe 41, the extraction driver is connected with filling breathing controller electricity, fills breathing controller and can control the required operating condition of extraction driver drive gas generator entering.
In the embodiment of the invention, the gas generator can extract the gas stored in the perfusion box body 1, the extraction driver can drive the gas generator to work, and the gas filter 40 can filter the gas to be introduced into the trachea of the isolated lung so as to prevent impurities in the gas from entering the trachea of the isolated lung. The trachea cannula 41 is adapted to the isolated lung, and the trachea cannula 41 has a trachea insertion end 42, that is, the trachea cannula 41 can be inserted into the trachea of the isolated lung through the trachea insertion end 42. The extraction driver is connected with the perfusion breathing controller electrically, and the perfusion breathing controller controls the extraction driver to work, so that the extraction driver can be matched with the gas generator, the gas in the perfusion box body 1 is pumped and then sent into the trachea of the isolated lung, the respiration of the isolated lung is assisted, and the function of the isolated lung in the operation process is maintained. Generally, the extraction driver and the gas generator are matched to send gas into the endotracheal frequency of the isolated lung, which is generally lower than the normal respiratory frequency of the isolated lung, and the gas is directly discharged into the storage perfusion box body 1 after the isolated lung breathes, so that the gas can be recycled.
As shown in fig. 6, the gas generator includes a generator housing 37, a gas extraction elastic ring 38 disposed inside the generator housing 37, and a filtering air inlet 39 disposed at the bottom of the generator housing 37; the extraction driver can drive the gas extraction elastic ring 37 to reciprocate in the generator shell 37, and when the gas extraction elastic ring 38 reciprocates in the generator shell 37, gas in the storage perfusion box body 1 can enter the generator shell 37 through the filtering gas inlet 39, and the gas in the generator shell 37 can enter the trachea of the isolated lung through the gas filter 40 and the trachea cannula 41.
In the embodiment of the present invention, the gas generator may adopt a form of a conventional foot-operated inflator, and specifically, the gas generator includes a generator housing 37, a gas extraction elastic ring 38 and a filtration air inlet 39, the gas extraction elastic ring 38 is disposed in the generator housing, the filtration air inlet 39 is an air inlet provided with a gas filtration membrane, the gas extraction elastic ring 38 can reciprocate in the generator housing 37, a length direction of the gas extraction elastic ring 38 is consistent with a length direction of the generator housing 37, the generator housing 37 may be generally made of a flexible membrane, which can accommodate the gas extraction elastic ring 38 in the generator housing 37 and can also realize reciprocating motion following the gas extraction elastic ring 38, and the gas extraction elastic ring 38 may generally adopt a spring ring. When the gas extraction elastic ring 38 is compressed in the generator shell 37, negative pressure can be generated in the generator shell 37, and gas in the storage perfusion box body 1 can enter the generator shell 37 through the filtering gas inlet 39; when the gas extraction spring ring 38 is reset, gas within the generator housing 37 can enter the gas filter 40. The gas filter 40 may be a conventional gas filter, such as a carbon dioxide absorbent.
After being fully filtered by the gas filter 40, the gas enters the trachea of the isolated lung through the trachea cannula 41, so that the cleanliness of the gas entering the trachea of the isolated lung is ensured. Generally, the diameter of the tracheal cannula 41 can be smaller than the trachea of the isolated lung, so that after the isolated lung breathes, the breathed gas or the non-breathed gas can be directly discharged into the storage perfusion box 1 through the gap between the tracheal cannula 41 and the trachea of the isolated lung. In specific implementation, the tracheal cannula 41 may further be provided with an exhaust valve 43, the exhaust valve 43 may be a commonly used air relief valve, that is, when the amount of air in the generator housing 37 is large, the exhaust valve 43 may exhaust excessive air, or the exhaust valve 43 may exhaust air in the isolated lung airway through the exhaust valve 43, so as to support the isolated lung breathing process.
When the gas generator adopts the above structure, the extraction driver includes an extraction elastic ring driving body 13 and an extraction elastic ring driving plate 14, the extraction elastic ring driving body 13 can adopt the form of a linear motor and the like, the extraction elastic ring driving plate 14 is connected with the output end of the extraction elastic ring driving body 13, the extraction elastic ring driving body 13 is matched with the extraction elastic ring driving plate 14 to realize the reciprocating motion of the driving gas extraction elastic ring 38, the extraction elastic ring driving body 13 is installed in the second box cover 11, and the extraction elastic ring driving plate 14 is located right above the gas extraction elastic ring 38. The specific form and driving process of the extracting elastic ring driving body 13 and the extracting elastic ring driving plate 14 cooperating with the driving gas extracting elastic ring 38 are similar to the specific form and driving process of the perfusion elastic ring driving body 9 and the perfusion elastic ring driving plate 10 driving the perfusion elastic ring 33, and reference may be specifically made to the above description, which is not repeated herein.
Claims (10)
1. An isolated lung storage perfusion device comprises a storage perfusion box body (1) which can be used for storing and transporting an isolated lung; the method is characterized in that: the storage perfusion box body (1) is internally provided with a perfusion circulation mechanism (5) which can be in adaptive connection with an isolated lung and a respiration circulation mechanism (6) which can be in adaptive connection with the isolated lung, the perfusion circulation mechanism (5) and the respiration circulation mechanism (6) are both electrically connected with a perfusion breath controller in the storage perfusion box body (1), and the perfusion circulation mechanism (5) and the respiration circulation mechanism (6) can be controlled to be in corresponding working states through the perfusion breath controller;
after the perfusion circulation mechanism (5) is in adaptive connection with the pulmonary artery and the pulmonary vein of the isolated lung, the perfusion breathing controller controls the perfusion circulation mechanism (5) to perform continuous perfusion on the connected isolated lung, and metabolites generated by the isolated lung can be removed in the perfusion process of the perfusion; the perfusion breathing controller controls the breathing circulation mechanism (6) to assist the in-vitro lung to breathe, so that the in-vitro lung can keep a normal breathing state.
2. The isolated lung storage perfusion apparatus of claim 1, wherein: set up insulating layer (2) in storage perfusion box (1) still set up the storage that can provide the required low temperature environment of separation lung storage and transportation and transport the refrigeration source.
3. The isolated lung storage perfusion apparatus of claim 2, wherein: store and transport refrigeration source including placing in storing the ice-cube that fills in box (1) or can realize the refrigerated semiconductor cooler of work, semiconductor cooler and control semiconductor cooler operating condition's pouring into breathe the controller and connect, it breathes the controller and passes through temperature sensor can gather the temperature of storing in real time and fill in box (1) to fill, fills and breathe the controller and pass through temperature sensor and semiconductor cooler and enable to store and fill the temperature environment that reaches separation lung transportation in box (1).
4. The isolated lung storage perfusion apparatus of claim 1, wherein: the perfusion circulating mechanism (5) comprises a perfusion injector (23) capable of containing perfusion liquid, a perfusion driver matched with the perfusion injector (23), and a pulmonary vein first connecting pipe (15), a pulmonary vein second connecting pipe (16), a pulmonary artery first connecting pipe (29) and a pulmonary artery second connecting pipe (30) which can be communicated with the perfusion injector (23);
through the first connecting pipe of pulmonary vein (15), pulmonary vein second connecting pipe (16) can with pulmonary vein adaptation connection, through the first connecting pipe of pulmonary artery (29), pulmonary artery second connecting pipe (30) can with pulmonary artery adaptation connection, through the perfusion driver can drive the perfusate in notes liquid ware (23) through the first connecting pipe of pulmonary artery (29), pulmonary artery second connecting pipe (30) get into the pulmonary artery of separation lung, and the perfusate that gets into in the separation lung can be through the first connecting pipe of pulmonary vein (15), pulmonary vein second connecting pipe (16) returns again to in notes liquid ware (23), in order to realize carrying out the perfusate circulation that lasts to the separation lung.
5. The isolated lung storage perfusion apparatus of claim 4, wherein: the pulmonary artery perfusion device is characterized by further comprising a perfusion liquid outlet pipe (26) and a perfusion liquid inlet pipe (19), wherein the perfusion liquid outlet pipe (26) and the perfusion liquid inlet pipe (19) are in adaptive connection with the perfusion device (23), one end of the perfusion liquid inlet pipe (19) is connected with the perfusion device (23), the other end of the perfusion liquid inlet pipe (19) is connected and communicated with a first pulmonary vein connecting pipe (15) and a second pulmonary vein connecting pipe (16), one end of the perfusion liquid outlet pipe (26) is connected with the perfusion device (23), and the other end of the perfusion liquid outlet pipe (26) is connected and communicated with a first pulmonary artery connecting pipe (29) and a second pulmonary artery connecting pipe (30);
a filtering remover (25) for removing the metabolic products in the isolated lung is arranged on the perfusion liquid outlet pipe (26), and a gas separator for discharging the gas in the perfusion liquid is arranged on the perfusion liquid inlet pipe (19).
6. The isolated lung storage perfusion apparatus of claim 5, wherein: a liquid outlet one-way valve (24) is arranged at the end part of the perfusion liquid outlet pipe (26) adjacent to the perfusion injector (23), and a liquid inlet one-way valve (22) is arranged at the end part of the perfusion liquid inlet pipe (19) adjacent to the perfusion injector (23);
the liquid ware of irritating (23) including can accept the perfusate receptacle (32) and set up in drive elasticity circle (33) of pouring into on the upper portion in the perfusate receptacle (32), drive elasticity circle (33) and perfusate receptacle (32) adaptation are connected to fill, can drive through the perfusion driver and fill drive elasticity circle (33) reciprocating motion in perfusate receptacle (32), fill when drive elasticity circle (33) reciprocating motion in perfusate receptacle (32), can make the perfusate in the perfusate feed liquor pipe (19) get into in the perfusate accepts chamber (34) of perfusate receptacle (32) lower part, and can make the perfusate in the perfusate accepts chamber (34) get into in pouring out the drain pipe (26).
7. The isolated lung storage perfusion apparatus of claim 5 or 6, wherein: the gas separator comprises a plurality of exhaust holes arranged on the perfusion liquid inlet pipe (19) and a water-blocking and air-permeable membrane (21) arranged in the exhaust holes, and the filtering remover (25) comprises an adsorption filtering membrane body (36) capable of adsorbing and separating metabolites.
8. The isolated lung storage perfusion apparatus of claim 4, or 5 or 6, wherein: the first connecting pipe clamp (17) is arranged on the first pulmonary vein connecting pipe (15), the second connecting pipe clamp (18) is arranged on the second pulmonary vein connecting pipe (16), the third connecting pipe clamp (27) is arranged on the first pulmonary artery connecting pipe (29), and the fourth connecting pipe clamp (28) is arranged on the second pulmonary artery connecting pipe (30).
9. The isolated lung storage perfusion apparatus of claim 1, wherein: breathe circulation mechanism (6) including can extract store fill gaseous gas generator in box (1), can drive gas generator's extraction driver and can carry out filterable gas filter (40) to gas generator extracted gas, gas filter's (40) gas outlet setting can be with the trachea of the trachea adaptation of separation lung put pipe (41), the extraction driver is connected with filling breathing controller electricity, fills breathing controller and can control and extract driver drive gas generator and get into required operating condition.
10. The isolated lung storage perfusion apparatus of claim 9, wherein: the gas generator comprises a generator shell (37), a gas extraction elastic ring (38) arranged in the generator shell (37) and a filtering gas inlet (39) arranged at the bottom of the generator shell (37); the extraction driver can drive the gas extraction elastic ring (37) to reciprocate in the generator shell (37), and when the gas extraction elastic ring (38) reciprocates in the generator shell (37), gas in the storage perfusion box body (1) can enter the generator shell (37) through the filtering gas inlet (39), and the gas in the generator shell (37) can enter the trachea of the isolated lung through the gas filter (40) and the trachea cannula (41).
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| CN201911248871.4A CN110786322A (en) | 2019-12-09 | 2019-12-09 | Isolated lung storage and perfusion device |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2418882Y (en) * | 1999-10-20 | 2001-02-14 | 张国报 | In vitro pulmonary circulation and perfusion device |
| CN101977649A (en) * | 2008-01-31 | 2011-02-16 | 特兰斯迈迪茨公司 | Systems and methods for ex vivo lung care |
| CN105660603A (en) * | 2016-01-11 | 2016-06-15 | 上海市肺科医院 | Lung preservation device for lung transplantation |
| CN106998676A (en) * | 2014-12-12 | 2017-08-01 | D·弗里德 | Apparatus and method for organ perfusion |
| DE102016225167A1 (en) * | 2016-12-15 | 2018-06-21 | Otto-Von-Guericke-Universität Magdeburg | Ex vivo pulmonary circulation and ventilation device and method for training surgical and minimally invasive surgical procedures |
| CN110506734A (en) * | 2019-08-26 | 2019-11-29 | 陈静瑜 | Isolated organ perfusion equipment |
-
2019
- 2019-12-09 CN CN201911248871.4A patent/CN110786322A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2418882Y (en) * | 1999-10-20 | 2001-02-14 | 张国报 | In vitro pulmonary circulation and perfusion device |
| CN101977649A (en) * | 2008-01-31 | 2011-02-16 | 特兰斯迈迪茨公司 | Systems and methods for ex vivo lung care |
| CN106998676A (en) * | 2014-12-12 | 2017-08-01 | D·弗里德 | Apparatus and method for organ perfusion |
| CN105660603A (en) * | 2016-01-11 | 2016-06-15 | 上海市肺科医院 | Lung preservation device for lung transplantation |
| DE102016225167A1 (en) * | 2016-12-15 | 2018-06-21 | Otto-Von-Guericke-Universität Magdeburg | Ex vivo pulmonary circulation and ventilation device and method for training surgical and minimally invasive surgical procedures |
| CN110506734A (en) * | 2019-08-26 | 2019-11-29 | 陈静瑜 | Isolated organ perfusion equipment |
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