CN115427574A - 工程化酸性α-葡萄糖苷酶变体 - Google Patents

工程化酸性α-葡萄糖苷酶变体 Download PDF

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Publication number
CN115427574A
CN115427574A CN202080097140.9A CN202080097140A CN115427574A CN 115427574 A CN115427574 A CN 115427574A CN 202080097140 A CN202080097140 A CN 202080097140A CN 115427574 A CN115427574 A CN 115427574A
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China
Prior art keywords
acid alpha
alpha glucosidase
seq
recombinant
sequence
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Pending
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CN202080097140.9A
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Chinese (zh)
Inventor
威廉·凯西·哈洛斯
雷切尔·凯思琳·博瑟姆
朱钰
庄静斌
尼基·德拉斯
吉伽特·W·哈思曼
穆莱·希沙姆·阿拉维伊斯梅利
大卫·威廉·霍曼
亚当·P·西尔弗曼
乔纳森·弗罗姆
杰西卡·P·劳
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Codexis Inc
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Codexis Inc
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Publication of CN115427574A publication Critical patent/CN115427574A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Neurology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202080097140.9A 2019-12-20 2020-12-18 工程化酸性α-葡萄糖苷酶变体 Pending CN115427574A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962951625P 2019-12-20 2019-12-20
US62/951,625 2019-12-20
PCT/US2020/066041 WO2021127457A1 (en) 2019-12-20 2020-12-18 Engineered acid alpha-glucosidase variants

Publications (1)

Publication Number Publication Date
CN115427574A true CN115427574A (zh) 2022-12-02

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US (2) US11970722B2 (https=)
EP (1) EP4077679A4 (https=)
JP (2) JP2023507575A (https=)
KR (1) KR20220129547A (https=)
CN (1) CN115427574A (https=)
AR (1) AR120858A1 (https=)
AU (1) AU2020408066A1 (https=)
BR (1) BR112022011760A2 (https=)
CA (1) CA3165484A1 (https=)
CL (1) CL2022001655A1 (https=)
CO (1) CO2022010224A2 (https=)
EC (1) ECSP22056585A (https=)
IL (1) IL294082A (https=)
MX (1) MX2022007399A (https=)
PE (1) PE20221266A1 (https=)
TW (1) TWI901622B (https=)
WO (1) WO2021127457A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117737040A (zh) * 2023-12-08 2024-03-22 深圳市新产业生物医学工程股份有限公司 α-葡萄糖苷酶突变体、试剂盒及其应用
WO2024188025A1 (zh) * 2023-03-14 2024-09-19 北京据德医药科技有限公司 一种重组酸性α-葡萄糖苷酶及其用途

Families Citing this family (7)

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SG11202100928QA (en) 2018-08-02 2021-02-25 Dyne Therapeutics Inc Muscle targeting complexes and uses thereof for treating facioscapulohumeral muscular dystrophy
US12018087B2 (en) 2018-08-02 2024-06-25 Dyne Therapeutics, Inc. Muscle-targeting complexes comprising an anti-transferrin receptor antibody linked to an oligonucleotide and methods of delivering oligonucleotide to a subject
JP2023507575A (ja) 2019-12-20 2023-02-24 コデクシス， インコーポレイテッド 操作された酸性アルファ－グルコシダーゼバリアント
CN115820609A (zh) * 2022-11-15 2023-03-21 新乡医学院 一种重组琼胶酶及其基因和应用
WO2024231820A1 (en) 2023-05-05 2024-11-14 Takeda Pharmaceutical Company Limited Treatment of pompe disease
EP4735591A2 (en) * 2023-06-30 2026-05-06 Crosswalk Therapeutics, Inc. Engineered acid alpha-glucosidase variants
WO2025004002A2 (en) 2023-06-30 2025-01-02 Takeda Pharmaceutical Company Limited Treatment of pompe disease

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Cited By (2)

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Publication number Priority date Publication date Assignee Title
WO2024188025A1 (zh) * 2023-03-14 2024-09-19 北京据德医药科技有限公司 一种重组酸性α-葡萄糖苷酶及其用途
CN117737040A (zh) * 2023-12-08 2024-03-22 深圳市新产业生物医学工程股份有限公司 α-葡萄糖苷酶突变体、试剂盒及其应用

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BR112022011760A2 (pt) 2022-08-30
US11970722B2 (en) 2024-04-30
US20240228997A1 (en) 2024-07-11
US12497605B2 (en) 2025-12-16
MX2022007399A (es) 2022-09-19
TW202136506A (zh) 2021-10-01
IL294082A (en) 2022-08-01
AR120858A1 (es) 2022-03-23
EP4077679A4 (en) 2024-04-24
TWI901622B (zh) 2025-10-21
AU2020408066A1 (en) 2022-06-30
CL2022001655A1 (es) 2023-02-24
ECSP22056585A (es) 2022-11-30
KR20220129547A (ko) 2022-09-23
EP4077679A1 (en) 2022-10-26
CO2022010224A2 (es) 2022-08-09
WO2021127457A1 (en) 2021-06-24
JP2025089465A (ja) 2025-06-12
PE20221266A1 (es) 2022-08-16
JP2023507575A (ja) 2023-02-24
CA3165484A1 (en) 2021-06-24
US20210189365A1 (en) 2021-06-24

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