CN115397978A - 用于动力学拆分的羧酸酯酶多肽 - Google Patents

用于动力学拆分的羧酸酯酶多肽 Download PDF

Info

Publication number
CN115397978A
CN115397978A CN202180027593.9A CN202180027593A CN115397978A CN 115397978 A CN115397978 A CN 115397978A CN 202180027593 A CN202180027593 A CN 202180027593A CN 115397978 A CN115397978 A CN 115397978A
Authority
CN
China
Prior art keywords
carboxylesterase
engineered
sequence
ala
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180027593.9A
Other languages
English (en)
Chinese (zh)
Inventor
拉玛·沃兰德里
杰西卡·安娜·胡尔塔克
奥里克·安东尼·索厄尔-肯士
南希塔·苏布兰马尼安
埃里卡·贝穆德斯
大卫·恩特韦斯特尔
美利莎·安·梅奥
詹姆斯·尼古拉斯·里金斯
斯科特·J·诺维克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Codexis Inc
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of CN115397978A publication Critical patent/CN115397978A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Prostheses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • External Artificial Organs (AREA)
CN202180027593.9A 2020-04-10 2021-04-06 用于动力学拆分的羧酸酯酶多肽 Pending CN115397978A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063008061P 2020-04-10 2020-04-10
US63/008,061 2020-04-10
PCT/US2021/025975 WO2021207208A1 (en) 2020-04-10 2021-04-06 Carboxyesterase polypeptides for kinetic resolution

Publications (1)

Publication Number Publication Date
CN115397978A true CN115397978A (zh) 2022-11-25

Family

ID=78024094

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180027593.9A Pending CN115397978A (zh) 2020-04-10 2021-04-06 用于动力学拆分的羧酸酯酶多肽

Country Status (7)

Country Link
US (1) US12612605B2 (https=)
EP (1) EP4133065A4 (https=)
JP (1) JP7747337B2 (https=)
CN (1) CN115397978A (https=)
CA (1) CA3179679A1 (https=)
IL (1) IL296983A (https=)
WO (1) WO2021207208A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112021020368A2 (pt) * 2019-04-11 2021-12-07 Bristol Myers Squibb Co Opções sintéticas para a fabricação de (6r,10s)-10-{4-[5-cloro-2-(4-cloro-1h-1,2,3-triazol-1-il)fenil]-6-oxo-1(6h)-pirimidinil}-1-(difluorometil)-6-metil-1,4,7,8,9,10-hexa-hidro-11,15-(meteno)pirazolo[4,3-b][1,7]diazaciclotetradecin-5(6h)-ona
CN115896065B (zh) * 2022-09-06 2023-08-11 江南大学 一种立体选择性羧酯酶、编码基因、载体及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1617931A (zh) * 2002-02-06 2005-05-18 联邦科学技术研究组织 具有脂肪酶活性的酯酶
JP2007167021A (ja) * 2005-12-26 2007-07-05 Tosoh Corp 光学活性エステルの製造方法
US20090311745A1 (en) * 2006-05-09 2009-12-17 B.R.A.I.N Ag Group of esterases for the enantioselective production of fine and speciality chemicals

Family Cites Families (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
FI964808A0 (fi) 1994-06-03 1996-12-02 Novo Nordisk Biotech Inc Puhdistetut Myceliophthora lakkaasit ja niitä koodaavat nukleiinihapot
CN101659926A (zh) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
FI104465B (fi) 1995-06-14 2000-02-15 Valio Oy Proteiinihydrolysaatteja allergioiden hoitamiseksi tai estämiseksi, niiden valmistus ja käyttö
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
KR100570935B1 (ko) 1997-01-17 2006-04-13 맥시겐, 인크. 반복적 서열 재조합에 의한 전세포 및 유기체의 개량 방법
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
WO1998031816A1 (en) 1997-01-17 1998-07-23 Regents Of The University Of Minnesota Dna molecules and protein displaying improved triazine compound degrading ability
US6537746B2 (en) 1997-12-08 2003-03-25 Maxygen, Inc. Method for creating polynucleotide and polypeptide sequences
US6484105B2 (en) 1998-04-02 2002-11-19 Maxyag, Inc. Method for obtaining a plant with a genetic lesion in a gene sequence
IL139093A0 (en) 1998-05-01 2001-11-25 Maxygen Inc Optimization of pest resistance genes using dna shuffling
IL140125A0 (en) 1998-06-17 2002-02-10 Maxygen Inc Method for producing polynucleotides with desired properties
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
JP2002522072A (ja) 1998-08-12 2002-07-23 マキシジェン， インコーポレイテッド 工業用化学薬品の製造のためのモノオキシゲナーゼ遺伝子のｄｎａシャッフリング。
JP2002526107A (ja) 1998-10-07 2002-08-20 マキシジェン， インコーポレイテッド マイコトキシンの解毒のための核酸を生成するためのｄｎａシャッフリング
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
JP4221100B2 (ja) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 半導体装置
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US20070065838A1 (en) 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
ES2341217T3 (es) 1999-01-19 2010-06-17 Maxygen, Inc. Recombinacion de acidos nucleicos mediada por oligonucleotidos.
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
JP2003524394A (ja) 1999-02-11 2003-08-19 マキシジェン， インコーポレイテッド ハイスループット質量分析法
AU3391900A (en) 1999-03-05 2000-09-21 Maxygen, Inc. Encryption of traits using split gene sequences
US6703240B1 (en) 1999-04-13 2004-03-09 Maxygar, Inc. Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6519065B1 (en) 1999-11-05 2003-02-11 Jds Fitel Inc. Chromatic dispersion compensation device
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
IL150291A0 (en) 2000-01-11 2002-12-01 Maxygen Inc Integrated systems and methods for diversity generation and screening
EP1272967A2 (en) 2000-03-30 2003-01-08 Maxygen, Inc. In silico cross-over site selection
JP2004508011A (ja) 2000-04-03 2004-03-18 マキシジェン，　インコーポレイテッド スブチリシン変異体
US7314974B2 (en) 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
US7747391B2 (en) 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
JP5319865B2 (ja) 2002-03-01 2013-10-16 コデクシス メイフラワー ホールディングス， エルエルシー 機能的生体分子を同定する方法、システム、およびソフトウェア
EP1488335A4 (en) 2002-03-09 2006-11-15 Maxygen Inc OPTIMIZING CROSSOVER POINTS FOR THE ADJUSTED EVOLUTION
EP2118281A2 (en) 2007-02-12 2009-11-18 Codexis, Inc. Structure-activity relationships
US8504498B2 (en) 2008-02-12 2013-08-06 Codexis, Inc. Method of generating an optimized, diverse population of variants
EP2250594B1 (en) 2008-02-12 2017-04-19 Codexis, Inc. Method of generating an optimized, diverse population of variants
DK3023494T3 (en) 2008-06-13 2019-02-25 Codexis Inc PROCEDURE FOR SYNTHESIS OF POLYNUCLEOTIDE VARIETIES
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
DK2726651T3 (en) 2011-06-28 2019-01-28 Codexis Inc PROTEIN INVARIANT GENERATION BY REGION SHUFFLING
US20150133698A1 (en) 2012-04-20 2015-05-14 Codexis, Inc. Production of fatty alcohols from engineered microorganisms
US9665694B2 (en) 2013-01-31 2017-05-30 Codexis, Inc. Methods, systems, and software for identifying bio-molecules with interacting components
US9453018B2 (en) 2014-10-01 2016-09-27 Bristol-Myers Squibb Company Pyrimidinones as factor XIa inhibitors
CN111465688A (zh) 2017-12-13 2020-07-28 葛兰素史密斯克莱知识产权发展有限公司 羧酸酯酶催化剂
JP2021506252A (ja) 2017-12-13 2021-02-22 コデクシス， インコーポレイテッド アミドカップリングのためのカルボキシエステラーゼポリペプチド

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1617931A (zh) * 2002-02-06 2005-05-18 联邦科学技术研究组织 具有脂肪酶活性的酯酶
JP2007167021A (ja) * 2005-12-26 2007-07-05 Tosoh Corp 光学活性エステルの製造方法
US20090311745A1 (en) * 2006-05-09 2009-12-17 B.R.A.I.N Ag Group of esterases for the enantioselective production of fine and speciality chemicals

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DA SILVA, A.C.R.等: "carboxylesterase [Xanthomonas campestris pv. campestris str. ATCC 33913], Accession No.: AAM42784.1", 《GENBANK数据库》, 31 January 2014 (2014-01-31) *
MELINE, V.等: "carboxylesterase [Xanthomonas campestris], Accession No.: RJU12542.1", 《GENBANK数据库》, 1 October 2018 (2018-10-01) *

Also Published As

Publication number Publication date
EP4133065A1 (en) 2023-02-15
JP2023521972A (ja) 2023-05-26
US20230159904A1 (en) 2023-05-25
CA3179679A1 (en) 2021-10-14
WO2021207208A1 (en) 2021-10-14
IL296983A (en) 2022-12-01
JP7747337B2 (ja) 2025-10-01
EP4133065A4 (en) 2024-07-10
US12612605B2 (en) 2026-04-28

Similar Documents

Publication Publication Date Title
CN111032682B (zh) 用于工业生物催化的工程化转氨酶多肽
US12410410B2 (en) Ketoreductase polypeptides for the synthesis of chiral compounds
CN109715817B (zh) 用于化合物的羟基化的生物催化剂和方法
CN105164263A (zh) 用于工业生物催化的工程化转氨酶多肽
CN109863162B (zh) 工程化亚胺还原酶和用于酮和胺化合物的还原胺化的方法
CN110831618A (zh) 酮还原酶多肽及多核苷酸
CN107002050A (zh) 工程化亚胺还原酶和用于酮和胺化合物的还原胺化的方法
CN114127272B (zh) 工程化亚胺还原酶和用于酮和胺化合物的还原胺化的方法
WO2021207200A2 (en) Engineered transaminase polypeptides
JP7644520B2 (ja) アミドカップリングのためのカルボキシエステラーゼポリペプチド
JP7747337B2 (ja) 速度論的光学分割のためのカルボキシエステラーゼポリペプチド
CN115175997B (zh) 用于化学化合物的羟基化的生物催化剂和方法
CA3155659A1 (en) Ketoreductase polypeptides and polynucleotides
CN116731991A (zh) 单胺氧化酶及其应用

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination