CN115397831B - 包含核酸寡聚物的组合物 - Google Patents

包含核酸寡聚物的组合物

Info

Publication number
CN115397831B
CN115397831B CN202180028331.4A CN202180028331A CN115397831B CN 115397831 B CN115397831 B CN 115397831B CN 202180028331 A CN202180028331 A CN 202180028331A CN 115397831 B CN115397831 B CN 115397831B
Authority
CN
China
Prior art keywords
group
nucleic acid
solution
methionine
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202180028331.4A
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English (en)
Chinese (zh)
Other versions
CN115397831A (zh
Inventor
森山悠也
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sumitomo Chemical Co Ltd
Original Assignee
Sumitomo Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sumitomo Chemical Co Ltd filed Critical Sumitomo Chemical Co Ltd
Publication of CN115397831A publication Critical patent/CN115397831A/zh
Application granted granted Critical
Publication of CN115397831B publication Critical patent/CN115397831B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00Processes for the preparation of sugar derivatives
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/31Chemical structure of the backbone
    • C12N2310/315Phosphorothioates
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/55Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN202180028331.4A 2020-04-14 2021-03-31 包含核酸寡聚物的组合物 Active CN115397831B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020072234 2020-04-14
JP2020-072234 2020-04-14
PCT/JP2021/014017 WO2021210409A1 (ja) 2020-04-14 2021-03-31 核酸オリゴマーを含む組成物

Publications (2)

Publication Number Publication Date
CN115397831A CN115397831A (zh) 2022-11-25
CN115397831B true CN115397831B (zh) 2026-02-24

Family

ID=78084944

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180028331.4A Active CN115397831B (zh) 2020-04-14 2021-03-31 包含核酸寡聚物的组合物

Country Status (6)

Country Link
US (1) US20230192755A1 (https=)
EP (1) EP4137501A4 (https=)
JP (1) JP7699581B2 (https=)
KR (1) KR20220167282A (https=)
CN (1) CN115397831B (https=)
WO (1) WO2021210409A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4368627A4 (en) * 2021-07-06 2025-10-08 Sumitomo Chemical Co COMPOSITION CONTAINING A NUCLEIC ACID OLIGOMER
US12509485B2 (en) * 2022-03-23 2025-12-30 Sumitomo Chemical Company, Limited Method for producing nucleic acid oligomer

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003005822A1 (en) * 2001-07-11 2003-01-23 Isis Pharmaceuticals, Inc. Enhancement of the stability of oligonucleotides comprising phosphorothioate linkages by addition of water-soluble antioxidants

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3687808A (en) 1969-08-14 1972-08-29 Univ Leland Stanford Junior Synthetic polynucleotides
JPH07170981A (ja) * 1993-12-17 1995-07-11 Res Dev Corp Of Japan オリゴヌクレオチドの製造法
JP4476386B2 (ja) 1999-08-03 2010-06-09 長谷川香料株式会社 環状ジスルフィド類の製法
US6586586B1 (en) * 2000-01-31 2003-07-01 Isis Pharmaceuticals, Inc. Purification of oligonucleotides
JP4580870B2 (ja) * 2003-09-02 2010-11-17 株式会社キラルジェン リボヌクレオチド又はリボヌクレオチド誘導体の製造方法
AU2005275801B2 (en) 2004-08-26 2012-05-10 Nippon Shinyaku Co., Ltd. Phosphoramidite compound and method for producing oligo-RNA
JP5317836B2 (ja) 2009-06-12 2013-10-16 住友精化株式会社 アルキルスルフィド化合物の製造方法
MX370398B (es) 2011-08-25 2019-12-11 Bonac Corp Compuesto de glicosido, metodo para producir tioeter, eter, metodo para producir eter, metodo para producir un compuesto de glicosido, metodo para producir acido nucleico.
EP3159407A1 (en) 2015-10-23 2017-04-26 Silence Therapeutics (London) Ltd Guide rnas, methods and uses
TW201734026A (zh) * 2015-12-28 2017-10-01 昭和電工股份有限公司 分離分析法
LT3576753T (lt) * 2017-02-03 2024-07-25 Laboratoires Kôl Sterili emulsija, apimanti stabilų fosforotioato oligonukleotidą
KR102670605B1 (ko) 2018-04-24 2024-05-29 스미또모 가가꾸 가부시끼가이샤 아미다이트 화합물 및 그 화합물을 사용한 폴리뉴클레오티드의 제조 방법
JP7431498B2 (ja) 2018-11-02 2024-02-15 株式会社東海理化電機製作所 電子機器、およびフレキシブルプリント基板
EP3950698A4 (en) * 2019-03-28 2023-01-25 Ajinomoto Co., Inc. METHOD OF PREPARING AN OLIGONUCLEOTIDE WITH PHOSPHOROTHIOATE SITE

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003005822A1 (en) * 2001-07-11 2003-01-23 Isis Pharmaceuticals, Inc. Enhancement of the stability of oligonucleotides comprising phosphorothioate linkages by addition of water-soluble antioxidants

Also Published As

Publication number Publication date
EP4137501A4 (en) 2024-05-22
JP7699581B2 (ja) 2025-06-27
US20230192755A1 (en) 2023-06-22
WO2021210409A1 (ja) 2021-10-21
EP4137501A1 (en) 2023-02-22
KR20220167282A (ko) 2022-12-20
JPWO2021210409A1 (https=) 2021-10-21
CN115397831A (zh) 2022-11-25

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