CN115353998A - EM (effective microorganisms) culture method and application of fermentation product thereof in composting and deodorizing of breeding houses - Google Patents
EM (effective microorganisms) culture method and application of fermentation product thereof in composting and deodorizing of breeding houses Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims description 39
- 230000004151 fermentation Effects 0.000 title claims description 38
- 244000005700 microbiome Species 0.000 title claims description 15
- 238000009395 breeding Methods 0.000 title abstract description 11
- 230000001488 breeding effect Effects 0.000 title abstract description 11
- 238000009264 composting Methods 0.000 title description 7
- 238000012136 culture method Methods 0.000 title description 7
- 230000001877 deodorizing effect Effects 0.000 title description 6
- 241000894006 Bacteria Species 0.000 claims abstract description 36
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 229910052613 tourmaline Inorganic materials 0.000 claims abstract description 23
- 229940070527 tourmaline Drugs 0.000 claims abstract description 23
- 239000011032 tourmaline Substances 0.000 claims abstract description 23
- 239000000843 powder Substances 0.000 claims abstract description 19
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 15
- 241000186361 Actinobacteria <class> Species 0.000 claims abstract description 13
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 13
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 13
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 12
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 12
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 12
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 12
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 12
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 12
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 12
- 229930006000 Sucrose Natural products 0.000 claims abstract description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 11
- 230000001580 bacterial effect Effects 0.000 claims abstract description 11
- 239000005720 sucrose Substances 0.000 claims abstract description 9
- 238000012258 culturing Methods 0.000 claims abstract 5
- 239000002994 raw material Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 13
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 229960004793 sucrose Drugs 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000002131 composite material Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 239000012263 liquid product Substances 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 2
- 239000002361 compost Substances 0.000 abstract description 7
- 241000255925 Diptera Species 0.000 abstract description 6
- 238000004332 deodorization Methods 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 235000019750 Crude protein Nutrition 0.000 abstract description 2
- 244000052616 bacterial pathogen Species 0.000 abstract description 2
- 150000001720 carbohydrates Chemical class 0.000 abstract description 2
- 239000001913 cellulose Substances 0.000 abstract description 2
- 229920002678 cellulose Polymers 0.000 abstract description 2
- 235000013601 eggs Nutrition 0.000 abstract description 2
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 229920005610 lignin Polymers 0.000 abstract description 2
- 239000003085 diluting agent Substances 0.000 description 5
- 230000007613 environmental effect Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241001494479 Pecora Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000003608 fece Anatomy 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 239000010871 livestock manure Substances 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 229910018072 Al 2 O 3 Inorganic materials 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- 229910018068 Li 2 O Inorganic materials 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 229910004298 SiO 2 Inorganic materials 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007366 host health Effects 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000000077 insect repellent Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-O oxonium Chemical compound [OH3+] XLYOFNOQVPJJNP-UHFFFAOYSA-O 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 229910052604 silicate mineral Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C12R2001/10—Bacillus licheniformis
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Abstract
The invention provides a culture medium composed of sucrose, tourmaline powder and the like, and the culture medium is used for culturing an EM microbial inoculum formed by combining baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria, actinomycetes, bacillus licheniformis and the like to obtain an original bacterial solution which can be applied to compost and deodorization of breeding houses, can degrade crude protein, cellulose, lignin and saccharides in the compost, improve the content of quick-acting nutrients, inhibit the growth of harmful bacteria, effectively remove odor and peculiar smell, kill worm eggs, repel mosquitoes and flies and effectively prevent the infection of germs and diseases in breeding places.
Description
The technical field is as follows:
the invention relates to the technical field of microbial fermentation culture, in particular to an EM (effective microorganisms) culture medium and application of a fermentation product thereof in composting and deodorizing of a breeding colony.
Background art:
many useful microorganisms exist in nature, and are widely applied to agriculture, industry, environmental protection and the like. Among the numerous microorganisms, some of them have important roles in environmental protection, soil improvement, maintenance of host health, and the like, and are called EM (effective microorganisms).
EM bacteria comprise photosynthetic bacteria, lactic acid bacteria, saccharomycetes, actinomycetes and the like, and by adopting a proper proportion and a unique fermentation process, aerobic and anaerobic beneficial microorganisms which are carefully screened are mixed and cultured to form various microbial communities, and beneficial substances and secretion substances thereof generated in the growth process of the microbial communities become respective or mutual growth substrates (food), so that a complex and stable microecological system is formed through the symbiotic proliferation relationship, and the powerful and unique advantages with various functions are formed. On one hand, the EM can degrade crude protein, cellulose, lignin and saccharides in the compost to generate easily utilized inorganic nutrients, humus and the like, so that the content of available nutrients is increased, and the absorption and utilization of crops are facilitated; on the other hand, the growth of harmful bacteria and the killing of worm eggs can be gradually and effectively inhibited along with the propagation of EM bacteria in the composting process, thereby not only reducing the generation of harmful substances and the problem of environmental odor, but also inhibiting the problem of soil-borne diseases caused by the harmful bacteria entering the soil. The odor and the peculiar smell can be effectively removed when the mosquito-repellent incense is applied to a breeding colony house, mosquitoes and flies can be repelled, the infection of germs and diseases in a breeding place can be effectively prevented, and the breeding space is cleaned.
In view of the great advantages of EM microbial inoculum in application, various technical products related to EM microbial inoculum are produced at present, and the culture medium and the culture method of liquid and solid microbial inoculum are also diversified.
Tourmaline (tourmaline) is a boron-containing cyclic silicate mineral with additional anions, and is developed and applied in the aspects of environmental protection, medical care, textile, coating and the like at present.
At present, no report related to applying tourmaline powder to EM microbial inoculum culture to promote EM microbial inoculum growth exists.
The invention content is as follows:
the technical problem to be solved by the invention is as follows: how to utilize tourmaline powder to promote the growth of EM microbial inoculum and improve the function of EM microbial inoculum in the deodorization of compost and breeding houses.
The main chemical component of tourmaline is SiO 2 、FeO、Fe 2 O 3 、B 2 O 3 、Al 2 O 3 、Na 2 O、MgO、Li 2 O、MnO 2 And the like. The crystal structure of tourmaline determines its characteristics of permanent electrification and permanent retention of positive and negative electrodes, and has the characteristic of forming electric field, and tourmaline has permanent electrode and reacts with water molecule to ionize water molecule to form H + And OH - 。H + Will form hydronium ion (H) 3 O + ) Or form H 2 ，OH - Formation of hydrated hydroxyl ion H 3 O 2 - ，H 3 O 2 - The negative ions emitted into the air are the air negative ions; the tourmaline has the characteristic of radiating far infrared rays with the wavelength of 4-18 mu m, and also has surface activity and adsorption performance. Due to the characteristics of tourmaline, a certain amount of tourmaline powder is added into the culture medium of the EM microbial inoculum, so that the dissolved oxygen in the culture medium can be increased, and the growth of the EM microbial inoculum is promoted. The electric field property and far infrared ray property of tourmaline can also stimulate the growth of EM microbial inoculum.
The invention uses an EM microbial inoculum ground fermentation tank (authorization notice number: CN 214781861U) for culture. The floor fermentation tank integrates the tank bodies and functions of a plurality of different fermentation processes into one tank to obtain the fermentation tank capable of performing full-automatic temperature rise and constant temperature control and aerobic and anaerobic alternate fermentation, and the whole process of fermentation such as material mixing, stirring and heating, aerobic fermentation, anaerobic fermentation, material circulation, discharging and the like is realized. The floor fermentation system moves the EM microbial inoculum fermentation link to the field and beside the farm, saves a large amount of factory production cost, simultaneously saves the cost of links such as packaging, transportation, marketing and the like, realizes zero distance of production and application, and reduces the application cost by more than 80%.
The technical scheme of the invention is as follows:
the EM bacteria culture medium is composed of the following raw materials in percentage by mass:
5 to 12.5 percent of cane sugar, 0.5 to 2 percent of peptone, 0.1 to 0.2 percent of sodium chloride, 0.1 to 0.2 percent of dipotassium phosphate, 0.05 to 0.2 percent of magnesium sulfate, 0.01 to 0.05 percent of tourmaline powder and water which are added to make up to 100 percent; the powder in the raw materials is crushed to be less than or equal to 200 meshes.
The EM bacterium culture method comprises the following steps:
(1) Preparing an EM (effective microorganism) culture medium consisting of sucrose, tourmaline powder and the like in a fermentation tank of a floor fermentation system;
(2) Pouring composite EM strain composed of baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria and actinomycetes into a culture medium, adding all the raw materials, fully and uniformly stirring, and covering and sealing by using a cover;
(3) Covering a cover, fermenting for 12 hours to 48 hours at the constant temperature of 38 ℃, fermenting for 3 days to 4 days at the normal temperature, then fermenting for 15 days to 25 days in a closed manner, stopping fermentation when the pH value reaches below 3.8, adding the bacillus licheniformis fermentation liquor which is fermented separately, and obtaining the original bacterial liquid product.
The EM strain consists of baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria, actinomycetes and bacillus licheniformis.
The general method for applying the prepared raw bacterial liquid product to compost and deodorization of breeding houses comprises the following steps: when composting, adding 100 kg of water to dilute 5-10 kg of the original bacteria liquid, uniformly splashing the diluted bacteria liquid on a fertilizer pile of 5m & lt 3 & gt, uniformly stirring the bacteria liquid, using the bacteria liquid for the first time for composting, and then using the bacteria liquid for the second time for fertilizer addition. For breeding houses: the 250 times of original bacterium liquid diluent is firstly sprayed on the ground, walls and roofs of the colony houses once a week, and after the bacterial liquid diluent is used for a period of time, the dilution times can be increased to 500 times, and the bacterial liquid diluent is sprayed once a week.
The beneficial technical effects of the invention are as follows:
the synergistic effect of the EM microbial inoculum and the tourmaline can promote the growth of beneficial microorganisms in compost and inhibit the growth of harmful microorganisms, thereby not only reducing the generation of harmful substances and the problem of environmental odor, but also inhibiting the problem of soil-borne diseases caused by the harmful bacteria entering the soil. In the culture process, the feed can also deodorize, repel mosquitoes and flies and improve the feeding environment.
The specific implementation mode is as follows:
the present invention will be described in detail with reference to examples and application data.
Example 1
The EM bacteria culture medium provided by the embodiment comprises the following raw materials in percentage by mass:
5% of sucrose, 2% of peptone, 0.1% of sodium chloride, 0.2% of dipotassium phosphate, 0.05% of magnesium sulfate, 0.05% of tourmaline powder and water for supplementing to 100%;
the powder in the raw materials is crushed to be less than or equal to 200 meshes.
The EM bacteria culture method of the culture medium comprises the following steps:
(1) Preparing an EM (effective microorganisms) culture medium consisting of sucrose, tourmaline powder and the like in a fermentation tank of a floor fermentation system;
(2) Pouring composite EM strain composed of baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria and actinomycetes into a culture medium, adding all the raw materials, fully and uniformly stirring, and covering and sealing by using a cover;
(3) Covering a cover, fermenting for 12 hours to 48 hours at the constant temperature of 38 ℃, fermenting for 3 days to 4 days at the normal temperature, then fermenting for 15 days to 25 days in a closed manner, stopping fermentation when the pH value reaches below 3.8, adding the bacillus licheniformis fermentation liquor which is fermented separately, and obtaining the original bacterial liquid product.
The EM strain consists of baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria, actinomycetes and bacillus licheniformis.
Example 2
The EM bacteria culture medium provided by the embodiment comprises the following raw materials in percentage by mass:
8 percent of sucrose, 1 percent of peptone, 0.2 percent of sodium chloride, 0.1 percent of dipotassium phosphate, 0.05 percent of magnesium sulfate, 0.03 percent of tourmaline powder and water for supplementing to 100 percent;
the powder in the raw materials is crushed to be less than or equal to 200 meshes.
The EM bacteria culture method of the culture medium comprises the following steps:
(1) Preparing an EM (effective microorganism) culture medium consisting of sucrose, tourmaline powder and the like in a fermentation tank of a floor fermentation system;
(2) Pouring composite EM strain composed of baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria and actinomycetes into a culture medium, adding all the raw materials, fully and uniformly stirring, and covering and sealing the culture medium by a cover;
(3) Covering a cover, fermenting for 12 hours to 48 hours at the constant temperature of 38 ℃, fermenting for 3 days to 4 days at the normal temperature, then fermenting for 15 days to 25 days in a closed manner, stopping fermentation when the pH value reaches below 3.8, adding the bacillus licheniformis fermentation liquor which is fermented separately, and obtaining the original bacterial liquid product.
The EM strain consists of baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria, actinomycetes and bacillus licheniformis.
Example 3
The EM bacteria culture medium provided by the embodiment comprises the following raw materials in percentage by mass:
12.5 percent of sucrose, 2 percent of peptone, 0.1 percent of sodium chloride, 0.1 percent of dipotassium phosphate, 0.2 percent of magnesium sulfate, 0.01 percent of tourmaline powder and water for complementing to 100 percent;
the powder in the raw materials is crushed to be less than or equal to 200 meshes.
The EM bacteria culture method of the culture medium comprises the following steps:
(1) Preparing an EM (effective microorganisms) culture medium consisting of sucrose, tourmaline powder and the like in a fermentation tank of a floor fermentation system;
(2) Pouring composite EM strain composed of baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria and actinomycetes into a culture medium, adding all the raw materials, fully and uniformly stirring, and covering and sealing the culture medium by a cover;
(3) Covering a cover, fermenting for 12 hours to 48 hours at the constant temperature of 38 ℃, fermenting for 3 days to 4 days at the normal temperature, then fermenting for 15 days to 25 days in a closed manner, stopping fermentation when the pH value reaches below 3.8, adding the bacillus licheniformis fermentation liquor which is fermented separately, and obtaining the original bacterial liquid product.
The EM strain consists of baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria, actinomycetes and bacillus licheniformis.
Application data:
diluting the obtained stock solution with 5-10 kg water 100 kg, and sprinkling on 5m 3 The sheep manure is piled and evenly stirred, and the sheep manure is used for 1 time during the first composting and is used for 1 time during the later fertilizer adding. After fermenting for 24 hours, the odor of the excrement disappears, and the excrement has stronger fermentation taste when fermented to the third day. The sheep manure compost needs 30 to 50 days.
Diluting the prepared original bacterial liquid by 100 times, and deodorizing in a pig raising colony house, wherein the dosage of the original bacterial liquid per square meter is 0.5-1 kg. Deodorizing 2-3 times per week, deodorizing 1 time per week after odor is reduced, and gradually increasing to 10 days to 1-2 times per month. Can eliminate odor, improve livestock and poultry environment, and promote health of livestock and poultry. For example, the ammonia concentration in the colony house without the diluent and the colony house after the diluent is sprayed is 0.302mg/m 3 And 0.193mg/m 3 (ii) a The odor concentration is respectively 19 and less than 10, and the deodorization effect is obvious.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and do not limit the protection scope of the claims. It should be understood by those skilled in the art that the present invention may be modified or replaced with other equivalent embodiments, but the present invention is within the spirit and scope of the present invention.
Claims (5)
1. The EM (effective microorganism) culture medium is characterized by consisting of the following raw materials in percentage by mass:
5 to 12.5 percent of cane sugar, 0.5 to 2 percent of peptone, 0.1 to 0.2 percent of sodium chloride, 0.1 to 0.2 percent of dipotassium phosphate, 0.05 to 0.2 percent of magnesium sulfate, 0.01 to 0.05 percent of tourmaline powder and water which are added to make up to 100 percent; the powder in the raw materials is crushed to be less than or equal to 200 meshes.
2. The EM culture medium of claim 1, wherein the EM strain consists of baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria, actinomycetes, and Bacillus licheniformis.
3. A method for culturing EM strain by an EM strain culture medium is characterized by comprising the following steps:
(1) Preparing an EM (effective microorganism) culture medium consisting of sucrose, tourmaline powder and the like in a fermentation tank of a floor fermentation system;
(2) Pouring composite EM strain composed of baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria and actinomycetes into a culture medium, adding all the raw materials, fully and uniformly stirring, and covering and sealing by using a cover;
(3) Covering a cover, fermenting for 12 hours to 48 hours at the constant temperature of 38 ℃, fermenting for 3 days to 4 days at the normal temperature, then fermenting for 15 days to 25 days in a closed manner, stopping fermentation when the pH value reaches below 3.8, adding the bacillus licheniformis fermentation liquor which is fermented separately, and obtaining the original bacterial liquid product.
4. The method for culturing EM bacteria, according to claim 3, wherein: culturing was carried out using a grounded fermenter with an EM microbial inoculum.
5. The method for culturing EM bacteria, according to claim 3, wherein: firstly, respectively preparing fermentation liquor of composite EM strain consisting of baker's yeast, lactobacillus acidophilus, lactobacillus plantarum, photosynthetic bacteria and actinomycetes and bacillus licheniformis, and then mixing the fermentation liquor according to the following weight ratio: 7 parts of composite EM strain fermentation liquid and 3 parts of bacillus licheniformis fermentation liquid.
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