CN115335704A - 用于检测腺相关病毒的方法和试剂盒 - Google Patents

用于检测腺相关病毒的方法和试剂盒 Download PDF

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Publication number
CN115335704A
CN115335704A CN202180023044.4A CN202180023044A CN115335704A CN 115335704 A CN115335704 A CN 115335704A CN 202180023044 A CN202180023044 A CN 202180023044A CN 115335704 A CN115335704 A CN 115335704A
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CN
China
Prior art keywords
aav
binds
kit
reagent
capture reagent
Prior art date
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Pending
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CN202180023044.4A
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English (en)
Chinese (zh)
Inventor
王鹏
顾炳南
A·赛思
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza Houston Inc
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Lonza Houston Inc
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Application filed by Lonza Houston Inc filed Critical Lonza Houston Inc
Publication of CN115335704A publication Critical patent/CN115335704A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/10—Detection of antigens from microorganism in sample from host

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202180023044.4A 2020-05-12 2021-05-10 用于检测腺相关病毒的方法和试剂盒 Pending CN115335704A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063023629P 2020-05-12 2020-05-12
US63/023,629 2020-05-12
PCT/US2021/031577 WO2021231296A1 (en) 2020-05-12 2021-05-10 Methods and kits for detecting adeno-associated viruses

Publications (1)

Publication Number Publication Date
CN115335704A true CN115335704A (zh) 2022-11-11

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CN202180023044.4A Pending CN115335704A (zh) 2020-05-12 2021-05-10 用于检测腺相关病毒的方法和试剂盒

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US (1) US20230168250A1 (enExample)
EP (2) EP4143577B1 (enExample)
JP (1) JP7763771B2 (enExample)
KR (1) KR20230009377A (enExample)
CN (1) CN115335704A (enExample)
CA (1) CA3172980A1 (enExample)
IL (1) IL298134A (enExample)
WO (1) WO2021231296A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023139224A1 (en) 2022-01-20 2023-07-27 Sartorius Xell GmbH METHOD FOR THE DETECTION AND QUANTIFICATION OF ADENO-ASSOCIATED VIRUSES (AAVs) USING AN AFFINITY MATRIX
CN119452254A (zh) * 2022-04-18 2025-02-14 万迈医疗仪器有限公司 用于确定dna病毒中的dna浓度的方法
JP2026516022A (ja) * 2023-05-05 2026-05-19 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Ａａｖベクターのための標識化および抗薬剤抗体アッセイ

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019067982A2 (en) * 2017-09-29 2019-04-04 Massachusetts Eye And Ear Infirmary PRODUCTION OF ADENO-ASSOCIATED VIRUSES IN INSECT CELLS

Family Cites Families (10)

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AU4954796A (en) 1995-03-17 1996-10-08 Hisamitsu Pharmaceutical Co., Inc. Monoclonal antibody specifically recognizing adeno-associated virus cap protein
US7419817B2 (en) * 2002-05-17 2008-09-02 The United States Of America As Represented By The Secretary Department Of Health And Human Services, Nih. Scalable purification of AAV2, AAV4 or AAV5 using ion-exchange chromatography
EP1724359A1 (en) 2005-05-18 2006-11-22 PerkinElmer Life and Analytical Sciences B.V. Luciferase assay system
SG169395A1 (en) 2006-02-09 2011-03-30 Univ South Florida Detection of cancer by elevated levels of bcl-2
WO2016035197A1 (ja) 2014-09-05 2016-03-10 株式会社日立製作所 電気化学免疫センサ用カートリッジ及びそれを用いた測定装置
EP4134375A1 (en) 2015-07-30 2023-02-15 Massachusetts Eye & Ear Infirmary Ancestral adeno associated virus sequences and uses thereof
CN119242587A (zh) 2017-02-17 2025-01-03 隆萨有限公司 难以表达的蛋白质的多位点特异性整合细胞
GB2574561A (en) 2017-03-14 2019-12-11 Univ California Engineering CRISPR CAS9 immune stealth
CA3098566A1 (en) * 2018-04-29 2019-11-07 Zhuchun WU Systems and methods of spectrophotometry for the determination of genome content, capsid content and full/empty ratios of adeno-associated virus particles
US20220332798A1 (en) * 2019-10-02 2022-10-20 Oregon Health & Science University Production of anti-viral monoclonal antibodies by hydrodynamic-based transfection

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019067982A2 (en) * 2017-09-29 2019-04-04 Massachusetts Eye And Ear Infirmary PRODUCTION OF ADENO-ASSOCIATED VIRUSES IN INSECT CELLS

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BRITTNEY L. GURDA等: "Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8", JOURNAL OF VIROLOGY, vol. 86, no. 15, 16 May 2012 (2012-05-16), pages 7739 - 7751, XP055590574, DOI: 10.1128/JVI.00218-12 *

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Publication number Publication date
WO2021231296A1 (en) 2021-11-18
US20230168250A1 (en) 2023-06-01
CA3172980A1 (en) 2021-11-18
EP4692800A2 (en) 2026-02-11
JP2023525204A (ja) 2023-06-15
EP4143577B1 (en) 2025-12-10
EP4143577A1 (en) 2023-03-08
EP4692800A3 (en) 2026-04-22
IL298134A (en) 2023-01-01
KR20230009377A (ko) 2023-01-17
JP7763771B2 (ja) 2025-11-04

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