CN115323051A - 一种急性髓系白血病检测探针组合物及其应用 - Google Patents
一种急性髓系白血病检测探针组合物及其应用 Download PDFInfo
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Abstract
本发明属于分子生物学技术领域,具体涉及一种急性髓系白血病检测探针组合物及其应用。采用本发明提供的探针组合物检测急性髓系白血病,具有较好的灵敏度和准确度,扩增效率高,且操作简便,快速省时,可以实现在骨髓形态学和血常规检查之外对AML进行辅助诊断,特别是对于疾病后续的治疗方向和效果做出预判具有特殊的优势,可弥补临床AML在分子诊断、疾病演变、治疗、预后、用药等指导方面的不足。
Description
技术领域
本发明属于分子生物学技术领域,具体涉及一种急性髓系白血病检测探针组合物及其应用。
背景技术
急性髓系白血病(acute myeloid leukemia,AML)是一组异质性的侵袭性血液系统恶性肿瘤,其临床病程和治疗反应存在广泛差异。急性髓系白血病的特征是未成熟的骨髓前体的扩张,从而导致正常的造血功能受损,最终导致骨髓衰竭。它是由影响造血干细胞或祖细胞增殖和/或分化的遗传改变的逐步积累引起的。AML在发生时常伴随着高度异质的细胞遗传学和分子生物学异常,这些异常能够辅助诊断、评估预后,从而参与治疗决策。近年来,测序技术的进步使得急性髓系白血病(AML)复发突变的鉴定成为可能。通过AML的测序工作,一个广泛的体细胞突变目录逐步呈现出来。其中反复发生的突变显示出高度的重叠,而频率较低的突变则高度分化。这表明,反复发生的突变是导致疾病的真正致癌驱动突变,而低频突变则可能是不影响AML生物学的无关突变。
人类AML患者中发现的最常见的异常之一是在正常造血发育过程中起作用的转录因子和表观遗传调节因子的突变,包括融合蛋白(如:PML-RARa、RUNX1-RUNX1T1、CBFb-MYH11等)和AML相关基因的重要突变(如:TP53、CEBPA、FLT3、DNMT3A等)等。如,专利CN107964561A公开了检测CEBPA基因突变的引物及检测方法,进一步用于判断正常核型AML患者的预后情况,用于检测的准确度、特异性和灵敏度较高。专利CN108929912A则公开了一种用于筛查急性髓系白血病的引物、试剂盒以及方法,其包括用于扩增人CCN1基因的SNP位点rs2297141和rs6576776片段的引物组合物,通过对位点rs2297141,rs6576776单个核酸多态性检测与分析,实现汉族人群急性髓系白血病的早期筛查和易感性的风险评估。然而,现有的这些评估标志物并不能完全有效地预测AML患者的风险,原因如下:第一,约50%的AML患者核型正常,既无基因组结构变异也无已知的基因突变,这些患者的复发风险和生存时间用现有的标志物难以预测。第二,虽然有些基因突变对生存时间的分类效果在统计学上极其显著,但是这些基因的突变率极低,绝大部分患者中无该类基因的突变,因此无法根据基因突变预测这些患者的生存时间。第三,现有的评估标准(如:European Leukemia Netguideline)通常将患者归为高、中、低风险三类,绝大部分患者被归为中风险类,进而采用相似的治疗手段。然而,被归类为中风险类的患者对于联合化疗的治疗反应有好有坏,这表明,对于中风险类的患者,还应使用新的预后标志物进行细分,进而调整治疗方案,以达到更好的治疗效果。第四,不同的基因突变间往往存在共现或互斥的情况,因此很多现有的预测因子没有独立的预后效果。比如,NPM1突变且FLT3不突变的患者具有较好的预后。
因此,亟需提供一种急性髓系白血病检测灵敏度、准确度更高的探针组合物及其检测方法。
发明内容
为了解决现有技术中的上述问题,本申请提供了一种急性髓系白血病AML相关基因群检测捕获探针组合物及其应用。采用本发明所述的捕获探针组合物检测AML相关基因群,具有较好的灵敏度和准确度,扩增效率高,且操作简便,快速省时。
为了实现上述发明目的,本发明提供如下技术方案:
一方面,本发明提供了一种急性髓系白血病相关基因群检测的探针组合物,所述的探针组合物包括SEQ ID NO:1-SEQ ID NO:538所示的序列。
具体地,所述的探针组合物用于检测下表1所示的基因及热点突变位点:
表1.基因及热点突变位点
注:Nonsense,无义突变,突变可造成氨基酸编码提前终止,进而可能影响蛋白功能;Frameshift,移码突变,突变使翻译阅读框发生改变,从而导致氨基酸序列发生变化;Splice Site,剪接位点突变,经数据库预测可能影响剪接位点,造成转录产物异常。
另一方面,本发明提供了上述探针组合物在制备急性髓系白血病相关基因群检测产品中的应用。
具体地,所述的产品包括但不限于独立试剂、芯片或试剂盒。
又一方面,本发明提供了一种急性髓系白血病相关基因群检测的产品,所述的产品包括上述探针组合物。
具体地,所述的产品包括但不限于独立试剂、芯片或试剂盒。
又一方面,本发明提供了上述探针组合物或产品在检测急性髓系白血病相关基因群突变中的应用。
又一方面,本发明提供了一种检测急性髓系白血病相关基因群突变的方法,所述的方法为非疾病诊断和治疗方法,所述的方法包括利用上述探针组合物或产品对待测样本中急性髓系白血病相关基因群的突变进行检测。
具体地,所述的方法包括以下步骤:
(1)血液样本单个核细胞分离;
(2)样本DNA提取;
(3)文库构建;
(4)上机测序;
(5)数据分析。
与现有技术相比,本发明提供的试剂盒具有如下有益效果:
(1)采用本发明提供的探针组合物检测AML(急性髓系白血病)相关基因群,具有较好的灵敏度和准确度,扩增效率高,且操作简便,快速省时。
(2)利用本发明提供的探针组合物,可以实现在骨髓形态学和血常规检查之外对AML进行辅助诊断,特别是对于疾病后续的治疗方向和效果做出预判具有特殊的优势,可弥补临床AML在分子诊断、疾病演变、治疗、预后、用药等指导方面的不足。
附图说明
图1为IDH2:p.R140Q sanger测序峰图。
图2为DNMT3A:p.R882P sanger测序峰图。
图3为NPM1:p.W288Cfs*12sanger测序峰图。
图4为GATA1:p.H71R sanger测序峰图。
图5为TET2:p.H1778R sanger测序峰图。
具体实施方式
下面结合具体实施例,对本发明作进一步详细的阐述,下述实施例不用于限制本发明,仅用于说明本发明。以下实施例中所使用的实验方法如无特殊说明,实施例中未注明具体条件的实验方法,通常按照常规条件,下述实施例中所使用的材料、试剂等,如无特殊说明,均可从商业途径得到。
实施例中未注明具体技术或条件者,均按照本领域内的文献所描述的技术或条件(如参考J.萨姆布鲁克等著,黄培堂等译的《分子克隆实验指南》,第三版,科学出版社)或者按照产品说明书进行。
1.试剂及仪器
(1)本申请所用的试剂信息如下表2所示:
表2.试剂
(2)本申请所用的仪器信息如下表3所示:
表3.仪器
实施例1.一种AML相关基因群检测的探针组合物及其试剂盒
(1)所述的探针组合物包括SEQ ID NO:1-SEQ ID NO:538所示的序列。
所述的探针组合物用于检测上表1所示的基因的热点突变(共786个)。
实施例2.一种AML相关基因群检测的方法
一、血液标本单个核细胞分离
准备15mL无菌塑料离心管放在试管架上并打开盖子,加入6-7mL淋巴细胞分离液;把抗凝标本(若标本量少于1mL,加入2mL盐水稀释颠倒混匀)缓慢倒入装有淋巴细胞分离液的15mL无菌塑料离心管中并盖上盖子,同时标记标本编号;然后离心(4℃2000r/min15min);准备15mL无菌塑料离心管放在相应试管架上并打开盖子,加入5mL无菌生理盐水;然后用一次性无菌塑料吸管充分吸取离心后的标本白细胞环并加入到盛有5mL无菌生理盐水的离心管中,同时标记标本编号;所有标本配平、离心(4℃1500r/min 5min);小心弃上清,向15mL无菌塑料离心管中加入9-10mL无菌生理盐水;离心(4℃1500r/min5min);小心弃上清,用新吸管把管底标本白细胞沉淀吸入到1.5mL无菌塑料离心管中,并用GS缓冲液补足体积到0.25mL,同时标记标本编号,准备用于DNA的提取。
二、血液标本DNA提取
向含有标本白细胞层的1.5mL经高压灭菌塑料离心管中加入20μL蛋白酶K(蛋白酶K可以提前加入到1.5mL无菌塑料离心管中);加入200μL GB缓冲液,盖好盖子,充分混匀15秒;放入水浴锅中,56℃孵育20分钟;取出离心管,小心开盖,加入200μL无水乙醇,盖好盖子,充分混匀15秒;转移到套有收集管的吸附柱CG2中,吸附柱CG2柱上标记标本编号,12000转/分钟离心2分钟;取出吸附柱CG2,换一新收集管,打开吸附柱CG2的盖子,向吸附柱CG2中加入500μL GDB缓冲液,盖好盖子,12000转/分钟离心1分钟;取出吸附柱CG2,再换一新收集管,打开吸附柱CG2的盖子,向吸附柱CG2中加入600μL PWB缓冲液,12000转/分钟离心1分钟;重复步骤上述步骤一次。取出吸附柱CG2,再换一新收集管,12000转/分钟离心2分钟;将吸附柱CG2置于一新1.5mL离心管中,在实验台开盖静置5分钟;加入65μL EB缓冲液,室温下孵育5分钟,12000转/分钟离心2分钟;1.5mL离心管内容物即为标本的DNA,在1.5mL离心管上标记好标本编号并盖好盖子(贴标签;将提取得到的DNA涡旋离心,测Nanodrop浓度和Qubit浓度,将浓度更新到标本信息表里,给DNA编号,暂放于4℃已待后续实验使用。
三、文库构建:
1、样本前处理:
组织DNA样本,若样本中无EDTA取所需量的组织DNA,用Nuclease-Free Water(NFWater)稀释到35μL。如果样本中有高浓度EDTA建议2倍体积的XP磁珠纯化后再建库。
2、样本酶切打断:
全程冰上加入试剂KAPA Frag Buffer(10×)-5μL、KAPA Frag Enzyme-10μL、稀释后样本-35μL。体系共50μL,涡旋混匀。具体反应程序如下表4:
表4
反应结束后将离心管取出放置于冰上,每个反应加入5μL Stop Solution;向每个反应中加入110μL已室温平衡的XP Beads(2×),涡旋混匀,室温孵育5min;瞬时离心,将EP管置于磁力架上吸附5min,弃上清;加入200μL新配的80%乙醇,静置30s,弃上清;重复上步一次,80%乙醇共洗2次;瞬时离心,用10μL枪头吸净液体,室温干燥3-5min;加入42μL的NFWater,重悬磁珠,室温孵育5min;瞬时离心,置于磁力架上直至液体澄清,吸取40μL上清至一新的PCR管中。
3、末端修复&加A:
反应体系:End Repair&A-Tailing Buffer-6μL、End Repair&A-Tailing Enzyme-4μL、上步反应产物-40μL。体系50μL,涡旋混匀。
反应条件:20℃-30min,65℃-30min,4℃-hold(特别注意:热盖70℃)。
4、接头连接:
反应体系:End repair and A-tailing reaction product-50μL、IDT UDI接头(15μM)-2μL,涡旋混匀,Ligation Buffer-26μL、DNA Ligase-2μL。体系80μL,涡旋混匀。
反应条件:放置于PCR仪上,20℃孵育20min(lid open)(有条件可适当延迟反应时间)。
将反应管瞬时离心,吸取全部反应液于含有64μL已室温平衡的SPBeads(0.8×)的1.5mL EP管中,涡旋混匀,室温孵育5-10min;瞬时离心,将EP管置于磁力架上吸附5min,弃上清;加入200μL新配的80%乙醇,静置30s,弃上清;重复上步一次,80%乙醇共洗2次;瞬时离心,用10μL枪头吸净液体,室温干燥3-5min;加入22μL的NFWater,重悬磁珠,室温孵育5min;瞬时离心,置于磁力架上直至液体澄清,吸取20μL上清至一新的PCR管中。
5、文库富集:
反应程序如下表5所示。
表5
将反应管瞬时离心,吸取全部反应液于含有40μL已室温平衡的SPBeads(0.8×)的1.5mL EP管中,涡旋混匀,室温孵育5min;瞬时离心,将EP管置于磁力架上吸附5min,弃上清;加入200μL新配的80%乙醇,静置30s,弃上清;重复上步一次,80%乙醇共洗2次;瞬时离心,用10μL枪头吸净液体,室温干燥3-5min;加入50μL的NFWater,重悬磁珠,室温孵育5min;瞬时离心,置于磁力架上直至液体澄清,吸取48μL上清至一新的EP管中,做好标记,即为得到的文库;取1μL的文库进行Qubit定量,取1μL的文库进行文库片段分析,记录文库浓度与产量。
四、杂交捕获:
1、DNA文库混合:
取一个新的1.5mL低吸附离心管,将不同index的文库混合,一个pool最多8个样本一起杂交;每个预文库样本投入300ng。
2、封闭文库并干燥,体系如下表6所示:
表6
封闭反应体系 | 单个用量(μL) |
DNA Library | 300ng/例 |
Human COT DNA | 5 |
NadPrepNanoBlockers | 2 |
充分混匀,使用提前预热的真空浓缩仪,55℃烘干。
3、探针与文库杂交,体系如下表7所示:
表7
杂交混合液 | 用量(μL) |
xGen 2×Hybridization Buffer | 8.5 |
xGen Hybridization Buffer Enhancer | 2.7 |
Probe探针 | 4 |
Nuclease-Free Water | 1.8 |
上述试剂加入到烘干的文库中,用移液器吹打混匀,室温孵育5-10min;充分涡旋混匀,瞬时离心;转移上述17μL杂交混合液至新的0.2mL低吸附PCR管中,瞬时离心。
反应条件:95℃-30s;65℃-4h;65℃-hold(特别注意:PCR仪热盖100℃)。
4、准备缓冲液,具体如下表8所示:
表8
试剂(试剂和NF Water提前平衡30min) | 母液用量(μL) | Water(μL) |
xGen 2×Bead Wash Buffer | 160 | 160 |
xGen 10×Wash Buffer 1 | 28 | 252 |
xGen 10×Wash Buffer 2 | 16 | 144 |
xGen 10×Wash Buffer 3 | 16 | 144 |
xGen 10×Stringent Wash Buffer | 32 | 288 |
每个反应分装110μL xGen 1×Wash Buffer 1至新的PCR管中,65℃孵育,使用前至少孵育15min;每个反应分装310μL xGen 1×Stringent Wash Buffer到两个PCR管中,每管155μL,置于65℃孵育,使用前至少孵育15min(置于热盖70℃、65℃孵育的另一PCR仪器中);剩余1×缓冲液放置于室温备用。
5、准备streptavidin Beads:
取出streptavidin beads并室温平衡至少30min,涡旋15s充分混匀磁珠;取适量磁珠至新的1.5mL低吸附离心管中,放置在磁力架上带澄清后弃去上清;每个捕获加入100μL xGen 1×Bead Wash Buffer,移液器缓慢吹打10次;瞬时离心,放入磁力架,待液体澄清,弃去上清;重复一次,从磁力架取下离心管,每个捕获加入100μL xGen 1×Bead WashBuffer,移液器缓慢吹打10次;瞬时离心,放入磁力架,待液体澄清,弃去上清;再重复一次,从磁力架取下离心管,每个捕获加入100μL xGen 1×Bead Wash Buffer,移液器缓慢吹打10次;瞬时离心,放入磁力架待液体澄清,弃去上清;每份磁珠中加入17μL磁珠重悬混合液,充分涡旋混匀,瞬时离心;将17μL磁珠重悬混合液,放置在PCR仪上孵育5min(可选)。
磁珠重悬混合液体系如下表9所示。
表9
磁珠重悬混合液 | 用量(μL) |
xGen 2×Hybridization Buffer | 8.5 |
xGen Hybridization Buffer Enhancer | 2.7 |
Nuclease-Free Water | 5.8 |
6、磁珠结合DNA:
杂交反应结束后,从PCR仪上取出含有杂交样本的PCR管;分装17μL重悬磁珠至上述含有杂交样本的PCR管中,充分涡旋混匀,瞬时离心;置于PCR仪上,65℃45min(热盖70℃,待PCR仪热盖温度完全降温至70℃时,再将样本置于PCR仪上)。(每间隔10min,涡旋混匀1次,保持样本处于悬浮状态)。
7、清洗杂交捕获文库:
从PCR仪取出PCR管,加100μL 65℃孵育的xGen 1×WashBuffer 1,移液器吹打10次混匀,避免出现气泡;瞬时离心,置于磁力架,待液体澄清,弃去上清;再次瞬时离心,用10μL枪头吸净残液;从磁力架取下离心管,加入150μL65℃孵育的xGen 1×StringentWashBuffer,移液器吹打10次混匀,注意避免出现气泡;65℃孵育5分钟;盖上热盖;瞬时离心,放入磁力架,待液体澄清,弃去上清;再次瞬时离心,用10μL枪头吸净残液;从磁力架取下离心管,加入150μL65℃孵育的xGen 1×StringentWash Buffer,移液器吹打10次混匀,注意避免出现气泡;65℃孵育5分钟;盖上热盖;瞬时离心,放入磁力架,待液体澄清,弃去上清;再次瞬时离心,用10μL枪头吸净残液;从磁力架取下离心管,加入150μL xGen 1×WashBuffer 1,充分涡旋混匀,室温孵育2min,隔30s震荡一次,以保持悬浮状态;瞬时离心,置于磁力架,待液体澄清,弃去上清;从磁力架取下离心管,加入150μL xGen 1×Wash Buffer2,充分涡旋混匀,室温孵育2min,隔30s震荡一次,以保持悬浮状态;瞬时离心,置于磁力架,待液体澄清,弃去上清;从磁力架取下离心管,加入150μL xGen 1×Wash Buffer 3,充分涡旋混匀,室温孵育2min,隔30s震荡一次,以保持悬浮状态;瞬时离心,置于磁力架,待液体澄清,弃去上清;瞬时离心,用10μL枪头吸净残留的xGen 1×Wash Buffer3,从磁力架取下离心管;加入20μL Nuclease-Free Water,移液器吹打10次混匀,以确保磁珠充分悬浮,转移至含有Post-capture PCR mix的0.2mL PCR管,涡旋混匀,轻微瞬时离心,避免磁珠沉淀。
8、post-capture PCR:
体系如下表10所示。
表10
Post-capture PCR混合液 | 单个用量(μL) |
2×KAPA HiFiHotStartReadyMix | 25 |
KAPA Primer(5μM/each Primer) | 5 |
杂交捕获文库 | 20 |
反应条件如下表11所示:
表11
取出AMPure XP beads室温平衡至少30min备用;将反应管瞬时离心,吸取全部反应液转入含有75μL(1.5×)已室温平衡的纯化Beads的1.5mL低吸附离心管中,涡旋混匀,室温孵育5-10min;瞬时离心,离心管置于磁力架上吸附2-5min,至液体澄清,弃去上清;保持离心管位于磁力架上,加入150μL 80%乙醇,静置1min,弃去上清;重复一次,加入150μL80%乙醇,静置1min,弃去上清;室温干燥1-3min,注意勿让磁珠过度干透;加入50μL EB洗脱,充分混匀磁珠,室温孵育5min;瞬时离心,置于磁力架上,至液体澄清,转移48μL上清至新的离心管中,做好标记,即为得到的文库;取1μL的文库进行Qubit定量,记录文库浓度与产量。
五、上机测序
1、试剂准备:
将试剂夹盒从冰箱-20℃中取出,在冰箱4℃解冻至少18个小时,也可以放入装有室温清水的水槽中解冻60分钟,注意不要将整个夹盒浸泡在水中;解冻后将夹盒翻转五次以混匀试剂,之后抬起夹盒边缘轻敲(左右两边都要需要)以减少气泡。(注:试剂夹盒中的试剂对光敏感,在解冻时注意避光。)将HT1从冰箱-20℃中取出,在室温下解冻。使用时放在冰盒上。将流动槽从冰箱4℃中取出,置于室温下30分钟。缓冲液夹盒在室温储存,在上机时取出即可。试剂夹盒、缓冲液盒、HT1、流动槽的标签揭下,贴在上机信息表上。
配制0.2N NaOH溶液(0.2mol/L NaOH溶液):5μL 10mol/L NaOH+45μL H2O涡离后得到50μL 1mol/L NaOH溶液。再取20μL 1mol/L NaOH溶液+80μL H2O涡离后得到100μL0.2N NaOH溶液。配制0.2mol/L Tris-HCL(pH7.0)溶液:20μL 1mol/L Tris-HCL+80μL H2O涡离后得到100μL 0.2mol/L Tris-HCL(pH7.0)。混pool并稀释至4nmol/L:根据“NextSeq上机信息表”先向1.5mL低吸附管中加入HT1,再加入文库。涡离后得到浓度为4nmol/L的Pooling文库。
2、测定Pooling文库浓度:
取3个500μL的qubit分析管,每管加入195μL的Equalbit 1X dsDNA HS WorkingSolution待用。再取2个分析管加入190μL的Equalbit 1X dsDNA HS Working Solution,分别加入10μL 1号标准品和2号标准品。涡旋离心后定标,若2号标准品的浓度为100ng/μL或99.8ng/μL即为定标通过;若低于99.8ng/μL,定标失败,应重新涡旋离心标准品或重新配制qubit标准品。定标通过后向待用的三管分别加入5μL本次测序的Pooling文库、上次测序的Pooling文库以及再上次测序的Pooling文库。涡旋离心后测定qubit浓度。该浓度将用于计算上机浓度。
变性文库:取5μL上述文库,加入到新的1.5mL低吸附管,再向其中加入5μL0.2NNaOH溶液,涡离后在室温下变性5min,5min后向其中加入5μL 0.2mol/L Tris-HCL(pH7.0),涡离后立即放冰上进行下一步。
3、稀释变性文库:
先向管中加入985μL HT1,再加入变性文库15μL,涡离后得到1mL 20pmol/L的稀释变性文库。稀释变性文库应放在冰上。
计算上机浓度与稀释变性文库加样体积:
注:上次测序与再上次测序的数据均可用来计算上机浓度,但计算时只应使用一次测序的数据。
4、制备上机文库:
根据计算好的稀释变性文库加样体积,先计算所需HT1体积:1300-稀释变性文库体积;配制上机文库时先加入HT1,再加入稀释变性文库,涡旋离心后即得到总体积为1300μL的上机文库,使用前应放冰上待用。
5、加样:
用1000μL的枪头刺破试剂夹盒10号孔,向其中加入上机文库,加样时沿管壁加样,注意不要打进气泡,枪头中可以适当残留一些上机文库。
6、上机测序:
更换流动槽:打开流动槽的锡纸包装,打开塑料盒包装,如果流动槽表面有灰尘或污渍,用无尘纸蘸取无水乙醇轻轻擦拭流动槽表面。取下清洗用的流动槽,安装上新的流动槽。
更换缓冲液盒以及清理废液盒:取下清洗用缓冲液盒,清空液体,倒扣在吸水纸上。换上新的缓冲液盒。清空废液盒中的废液,重新放回舱内。
更换试剂夹盒:从左侧舱取出清洗夹盒,清空液体,倒扣在吸水纸上。把已上样的试剂夹盒放入舱内。
复查运行设定与仪器自检:再次确认运行设定后选择“Next”,等待仪器完成自检,自检完成后选择“start”开始测序。
六、数据分析:
1、原始测序信号文件利用bcl2fastq程序转化成fastq格式文件;原始数据(fastq格式文件)经过fastp软件质控,过滤掉低质量、不确定碱基、含有接头的reads得到cleandata(fastq格式文件);利用BWA软件将质控合格的clean data比对到人类hg19参考基因组,得到BAM格式文件;利用sambamba软件去除BAM文件中的duplicate reads;利用VarDcct软件对BAM格式文件进行变异检测,得到VCF格式文件;利用ANNOVAR软件进行相关数据库注释(注释内容包括突变在基因组中的位置,关联的基因,基因外显子编号,核苷酸水平变异,对应的蛋白水平变异,该突变在dbSNP(http://www.ncbi.nlm.nih.gov/snp/),1000Genomes数据库(http://www.1000genomes.org/)和癌症突变数据库COSMIC(http://cancer.sanger.ac.uk/cosmic)中的注释,PolyPhen(http://genetics.bwh.harvard.edu/pph2/)和SIFT(http://sift.jcvi.org/)功能预测结果等)。
2、按照以下原则筛选突变位点:
(1)根据查阅文献指南等整理出的热点库进行注释,按照不同分级对热点突变判读为致病突变;
(2)根据突变所在的基因组位置和类型,过滤掉对蛋白产物序列不产生影响的突变;
(3)利用1000Genomes数据,注释每个突变在人群中的比例,如果比例小于等于1%则不认为是多态性位点;
(4)经过(3)后,一个突变满足“非多态性”且为特殊突变(无义/移码突变/插入/缺失(包括非移码突变)/经典剪接位点(±1/2时)/起始密码丢失/终止密码子丢失),则判读为与疾病可能相关;
(5)检索癌症数据库COSMIC,查询一个突变在COSMIC中是否有记载,其出现在血液系统肿瘤中;
(6)利用蛋白质功能预测软件PolyPhen-2等数据库预测该突变是否影响蛋白功能;
(7)如果经过(3)(5)(6)后,一个突变满足“非多态性”、“在血液系统肿瘤中记载过”和“影响蛋白功能”这三条中至少两条的,将被判为意义不明突变。
实验例1.重复性检测
根据实施例1提供的探针组合物,分别采用实施例2的检测方法检测AML患者样本2例,重复检测三次,其结果如下表12所示。
表12.重复性检测结果
由上表可知,本申请所述的探针组合物具有较好的重复性。
实验例2.灵敏度检测
根据实施例1提供的探针组合物,分别采用实施例2的检测方法提取的不同浓度的DNA样本进行检测。选择已知突变频率的阳性突变位点,按一定浓度梯度等比稀释样本(1;1:2;1:4;1:8;1:16;1:32;1:64),并保证总核酸量保持不变,常规实施该项目检测,其结果如下表13所示。
表13.灵敏度检测结果
由上表可知,本申请所述的探针组合物具有较好的灵敏性。
实验例3.准确性检测
选取临床样本2例,采用本申请所述的检测方法进行检测,与sanger测序结果(临床结果)进行比较,检测结果如下表14,sanger测序峰图如图1-5所示。
表14.准确性检测结果
突变位点 | 变异频率 | sanger测序 |
IDH2:p.R140Q | 9.6%,阳性 | 阳性 |
DNMT3A:p.R882P | 43.7%,阳性 | 阳性 |
NPM1:p.W288Cfs*12 | 44.0%,阳性 | 阳性 |
GATA1:p.H71R | 46.1%,阳性 | 阳性 |
TET2:p.H1778R | 62.0%,阳性 | 阳性 |
由上表可知,本申请所述的探针组合物具有较好的准确性。
本实施例sanger测序所用的引物序列如下表15所示:
表15
上述实验均经过中心伦理委员会批准,并经患者同意。
以上仅为本发明的实施方式,其描述较为具体和详细,但并不能因此而理解为对本发明专利范围的限制。应当指出的是,对于本领域的普通技术人员来说,在不脱离本发明构思的前提下,还可以做出若干变形和改进,这些均属于本发明的保护范围。
SEQUENCE LISTING
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<120> 一种急性髓系白血病检测探针组合物及其应用
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<213> 人工序列(Artificial Sequence)
<400> 20
aacaggctcc cctgcacttc cccatccggt agcaccacca cccagcctgc acccgatggg 60
gtccctgggc ctttggcaga tacctccctt gttactgctt ctgccaaggt gcttccaact 120
<210> 21
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 21
ccacagcctc tgctgccagc ccccagtggg agctcagccc caccgcaccc cgccaagatg 60
cccagtggca ccgagcagca aacagaaggg acttccgtta ccttctctcc tcttaagtca 120
<210> 22
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 22
ccgccacagc tggaacgaga gatggcctct ccacctgagt gcagcgagat gccccttgat 60
ctgtcctcca agtccaaccg ccagaagctt ccattgccga accagcgcaa gacacccccc 120
<210> 23
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 23
atgcctgtgt tgacccccgt gcacaccagc agcaaggccc tcctctccac agtcctgtct 60
aggtctcagc gcacaaccca ggctgccggt ggcaatgtca cctcctgcct gggctccact 120
<210> 24
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 24
tcctcgccct ttgtcatctt tcccgagatc gtgaggaatg gggacccgag cacctgggtg 60
aagaactcaa ctgcactgat cagcaccatt cctggcacct acgtgggagt ggccaaccca 120
<210> 25
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 25
gtgcctgcat ccctgctgct gaacaaagac cccaacctgg gcctcaaccg tgacccccgc 60
catctcccca agcaggagcc catctccatc attgatcaag gagagcctaa gggcactggt 120
<210> 26
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 26
gccacgtgtg gcaaaaaggg cagccaggct ggtgctgagg gacagccaag cacagtgaaa 60
cgatatactc cagcccgcat tgcccctggg ctgccagggt gccaaaccaa ggaactctct 120
<210> 27
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 27
cttgggcaca agtcagaaga caagcctgac gatccccagc caaaaatgga ctacgctggg 60
aacgtggcag aggctgaggg cttcttggtg cccctgagca gcccaggaga cgggctcaag 120
<210> 28
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 28
ttgtggaaac ccacggggcc ggcaaatatt tatccccggt gttcagtcaa tgggaaacct 60
accagcaccc aggtcctgcc tgttggctgg tccccgtacc accaggcgtc tctgctttcc 120
<210> 29
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 29
attggcattt ccagtgccgg gcagctgacc cccagtcagg gggcgcccat caggcccacc 60
agcgttgttt cggagttttc tggtgtgcca tctctcagct ccagcgaagc cgtgcacgga 120
<210> 30
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 30
cttcctgagg ggcaaccacg gcctgggggc tccttcgttc cagagcagga ccctgttaca 60
aagaacaaaa cttgccggat tgctgccaag ccttatgaag aacaagtcaa tcctgtcctc 120
<210> 31
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 31
ttgaccctca gccctcagac tgggaccctg gcactgtctg ttcagcctag cggtggggac 60
attcgaatga atcaggggcc tgaggaatca gagagccacc tctgctctga cagcactcct 120
<210> 32
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 32
aagatggaag gcccccaggg ggcttgtggc ctgaagctgg caggagacac gaagcctaag 60
aaccaagtgc tggccaccta catgtcccat gagctggtcc tggccacccc ccagaacctg 120
<210> 33
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 33
cctaagatgc ctgagctgcc tttgctacct cacgacagcc accccaagga acttatattg 60
gacgtggttc cgagcagcag gaggggctcc agcacagagc gcccacagct tggaagccag 120
<210> 34
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 34
gtggatctgg ggcgagtgaa aatggagaag gtggatggtg atgtggtctt caatttagcc 60
acctgcttcc gggctgatgg cctcccagtg gctccccaga ggggccaagc tgaagttcgg 120
<210> 35
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 35
gctaaggccg ggcaggctcg agtgaaacag gaaagcgtag gggtctttgc ttgcaagaac 60
aagtggcagc cagatgatgt gacggaatct ctgccgccca agaagatgaa gtgcggcaaa 120
<210> 36
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 36
gagaaggaca gtgaagagca gcagctccag ccacaagcca aggccgtggt ccggagttcc 60
cacagaccca aggtgagtgc tgagctaagg tccagggtgg ggccgagatg ccgctggtct 120
<210> 37
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 37
cttcccgcat ctgacagcgc cgaggccagc aacagcaggg ccgactgctc ctggactcca 60
ctcaacaccc aaatgagcaa acaggttgac tgctcacccg ccggagtaaa ggctttggac 120
<210> 38
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 38
tctcggcaag gtgttggaga gaagaatact ttcattttgg caactctggg aactggagtc 60
cctgtggagg ggaccctgcc cctggttacc actaacttca gtcctctgcc agcccctatc 120
<210> 39
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 39
tgtccccctg ctcccggttc ggcctctgtg ccccactctg ttccagatgc attccaggtt 60
cccctctccg tccctgcccc agtcccccat tcagggcttg ttccagtcca agttgccact 120
<210> 40
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 40
tcggttccag ctccttcccc tcccttagca cctgtcccgg ctctggctcc agcgccaccg 60
tcagtgccca cgctcatctc tgactcgaac cccctttctg tttcggcctc agtcttggtg 120
<210> 41
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 41
cctgtgccag cttctgctcc cccttcaggc ccggttccct tgtcggctcc agctcctgcc 60
ccgctttcag tcccagtttc agctcctccc ttggctctca tccaggctcc tgtgccccct 120
<210> 42
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 42
tcagctccga ccttggttct cgctcccgtc cccactccgg ttctggctcc catgccagca 60
tccacgcctc cagcggcccc tgcccctccg tctgtgccca tgcccactcc aaccccatct 120
<210> 43
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 43
tccggcccac cttctacccc caccctcatc cccgcctttg ctcctacacc ggtgcctgca 60
cccaccccag cccccatctt tactccagcc cctacaccca tgcctgctgc cacgccagct 120
<210> 44
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 44
aaactgacct cagacctgac cctctacctc tccacagtgc cggaagctgc ccagtgaccc 60
ccaggaatcc accaagaaaa gccccagggg ggcttcagat tcaggaaaag agcacaatgg 120
<210> 45
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 45
agtcagggga aagcacaagc accggaagcc gacaaagccg gagtcccagt ctccaggaaa 60
acgagccgac agccacgagg aaggtaggcc ccgcggccct ggccctctgg gctgggctgc 120
<210> 46
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 46
cacagtggtg agggcagagg ggctcaggcg ctgactcctt cactctggaa aagtgagact 60
tcttcactgt ggaaagaaga tatgattgac ccctctgaag tctaaggtga gcgagcaagg 120
<210> 47
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 47
caaaccacac atcactccag gttccttgga aaagaaagca aagagcagtt tccgtgactt 60
tattcctgtg gttctgagca cccgcacgcg cagtcagtct ggtgagtgag actaaggtga 120
<210> 48
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 48
attcacttcg acaccatgct ttgtcctttg aagaagtacc tgaccatcct ctgtacatcc 60
catccacagg aagcatctgt agctcctttg ctggcatggc agacagtgac atgggaagcc 120
<210> 49
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 49
gaagccagga agtcttcccc acagaagaag aagaggaggt aacccccacc ccagctaagc 60
gtcgaaaggt gagaaagacc caacgggaca cccagtatcg cagccaccat gcccaggaca 120
<210> 50
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 50
aggacaagtc tctgctgagc cagggccgaa ggcacctgtg gcgagcccga gaaatgccct 60
ggaggacaga ggctgcccgg caaatgtggg acaccaatga ggaggaggag gaagaagagg 120
<210> 51
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 51
gggcctgctg aagaggaaga aacgaagacg gcagaagagc cgaaaatatc agactgggga 60
gtacctgaca gagcaagaag acgagcagcg gcggaaaggg agagcaggta aggctggcca 120
<210> 52
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 52
cttcagattt aaaggcccgt aagcagaaga cttcctcctc ccaaagtttg gagcaccgcc 60
tcaggaacag gaaccttctc ttgcccaaca aagtccaggg gatctcggat tcaccaaacg 120
<210> 53
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 53
gtttcctccc aaataacctg gaagagccag cctgccttga aaattcagaa aagccatcag 60
gaaaacgaaa gtgcaagacc aagcacatgg caaccgtctc agaagaggca aaggtgtgta 120
<210> 54
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 54
gctttccagg gcaaaggtcg ttggagccag cagaagacac gatctcccaa atctcccacc 60
ccagtgaaac ccacagaacc atgtacaccc tctaagtccc gaagtgccag ctcagaggag 120
<210> 55
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 55
gcctcagagt cacctacagc ccggcagatc cccccagagg cacgtcggct catagtgaac 60
aaaaatgctg gtgagaccct cctgcagagg gcggcgcgtc ttggctataa ggtaagggag 120
<210> 56
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 56
ctctctttgc tcatggagtt gtccctgtcc ccacaggatg ttgttctcta ctgcctccag 60
aaagacagtg aagatgtgaa tcaccgtgac aatgctggct acacagccct gcatgaggct 120
<210> 57
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 57
tgttcccggg gctggaccga catcctgaac atcctgctgg agcacggggc caacgtgaac 60
tgcagtgcgc aggacggcac gaggcaagag ggctgcatct ccccccagtc ccgccctcac 120
<210> 58
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 58
agttcccact tgggccctgt tatcctcacc ctgtatcacc tccacaggcc agttcatgat 60
gcggtggtca atgacaacct ggagaccatc tggctcctgc tgtcctatgg ggccgatccc 120
<210> 59
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 59
acactggcta cctactcggg tcagacagcc atgaagctgg ccagcagcga caccatgaag 60
cgctttctca gtggtaagca tggtccagac ttgacactgt tgtccttggg gccagcctct 120
<210> 60
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 60
tgcctgctct tctttcatca gatcacctct cggatcttca gggccgggca gagggtgatc 60
ccggtgtatc ctgggatttt tacagcagtt ctgtgttggg taagttttaa gtgagattct 120
<210> 61
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 61
acatgtatct gagtcctaat cccctatatg ctccccttac agaggaaaaa gacgggtttg 60
cctgtgacct cctacataat cctcctggga gctcagatca agaaggagac gatccgatgg 120
<210> 62
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 62
aggaggatga tttcatgttt gaactctcag acaagcctct tctcccttgc tacaacctcc 60
aagtgtcagt gtcccgcggg taagtgtccg agagatccac ggtgactgag tgtcaggatc 120
<210> 63
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 63
ccagcctcct tcaataactc ccatccttgc ctttgcaggc cctgcaactg gttcctcttt 60
tccgatgtct tgaagaggct gaagctttcc tcgaggatct ttcaggcccg gttcccgcac 120
<210> 64
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 64
tttgaaatca ccaccatgcc caaggccgag ttctacaggc aggtggcctc cagtcagctg 60
ctgacccctg ccgagaggcc tggaggcttg gacgacagat cccccccagg ctcctctgag 120
<210> 65
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 65
actgtggagc tggtgcggta cgagccagac ctacttcggc tcctagggtc cgaggtggaa 60
ttccagtctt gcaacagttg accgggaaaa cagcccctcc tcttctttct ccttccgagt 120
<210> 66
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 66
aacagaagaa gaagaaggag cgcacgtggg ccgaggccgc gcgcctggtg aggcggacag 60
ccgagggggg ctccgtgggg cccggggtgg ggggggctcg ccgcgcaccc ccccactggg 120
<210> 67
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 67
acgtttatat ttcttcaggt attagaaaac tactcggatg ctccaatgac accaaaacag 60
attctgcagg tcatagaggc agaaggacta aaggaaatga ggtttgtatt gttcttgttg 120
<210> 68
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 68
tgcttaacat gagggtttca taggatatgt gtctttctgt agttgtagtg atatgagtaa 60
gtacacctgt gtatgtattt gtgcttctgt gtctggtagt gagatggaac tatccatttc 120
<210> 69
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 69
ttacagtggg acttcccctc tcgcatgcct caatgctatg ctacattcca attcaagagg 60
aggagagggg ttgttttata aactgcctgg ccgaatcagc cttttcacgc tcaaggtaag 120
<210> 70
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 70
ttgtaatttg gctctgggca gaattcttgg catagtacgt gctttatgtg tgaactgaat 60
tatatttctt catcttaatt ttacaggtgt gagccactgc accaggcccc ttcatcttaa 120
<210> 71
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 71
ttttaatata tctttgaata aacaccattg tatgaacctg ctgtaagctt gggagtggtc 60
tgttagtcta cagcttgtgt ctgagatgtg ctaattgaat atttgctcag tacctcatct 120
<210> 72
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 72
taactgcctt tggctttatg ttgcttatcc ttcatagtat cttgttcatt ggccttttac 60
atccataggc atcacttctc tgatattcgt tgtgctcttt taatggatta atggtttgc 119
<210> 73
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 73
ctgccttgct ttaagaattt gtagggtttt gttcacctga gttgtacctt gctgtcacag 60
aaggatgccc tgcagtggtc tcgccatcca gctacagtgg agggagagga gccagaggac 120
<210> 74
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 74
acggctgatg tggagagctg tgggtctaat gaagccagca ctgtgagtgg tgaaaacgat 60
ggtaaggacc ctttaatgga tgggtgaggg agccacagca ggcactaggg actaaccttt 120
<210> 75
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 75
ctctcttcca gtatctcttg atgaaacatc ttcgaacgca tcctgttcta cagaatctca 60
gagtcgacct ctttccaatc ccagggacag ctacagagct tcctcacagg taaggaagag 120
<210> 76
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 76
tcttggaacg caggcgaaca aacaaaagaa aaagactggg gtgatgctgc ctcgagttgt 60
cctgactcct ctgaaggtaa acggggccca cgtggaatct gcatcaggta tgtgtaaact 120
<210> 77
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 77
gcggcttggt gatacttttg accagtggaa tgctgtgcct tcagggttct cgggctgcca 60
cgccgatggc gagagcggca gcccgtccag cagcagcagc ggctctctgg ccctgggcag 120
<210> 78
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 78
cgctgctatt cgtggccagg ccgaggtcac ccaggaccct gccccgctcc tgagaggctt 60
ccggaagcca gccacaggtg agtggcgtgg cacttatttc tctgcctgta aagggggccc 120
<210> 79
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 79
aaaaagctga aatctatacc ttgcttcaaa aatcataggt caaatgaagc gcaacagagg 60
ggaagaaata gattttgaga cacctgggtc cattcttgtc aacaccaacc tccgtgccct 120
<210> 80
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 80
gatcaactct cggaccttcc atgccttacc atcacacttc cagcagcagc tcctcttcct 60
cctgcctgaa gtagacagac aggtgcacat gggcagcctc ccctttgcct ctctctgggt 120
<210> 81
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 81
tgatccttct aggtggggac ggatggcctg ttgcgtctca gcagcagtgc actaaataac 60
gagtttttta cccatgcggc tcagagctgg cgggagcgcc tggctgatgg tatgtagact 120
<210> 82
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 82
tccataagac agacattaat atcccgaatg cacttactag aagaggttta tttctcccta 60
ggtgaattta ctcatgagat gcaagtcagg atacgacagg aaatggagaa ggaaaagaa 119
<210> 83
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 83
tggaacaatg gaaagaaaag ttctttgaag actactatgg acagaagtaa ggcagttgga 60
gctatgagtc ctggtctggg gttttgaggg gatagaggta gatggtctca aaatagcata 120
<210> 84
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 84
tccactctgg cctgaaactg atggctgtga ttttgatttg caggctgggt ttgaccaaag 60
aagagtcatt gcagcagaac gtgggccagg aggaggctga aatcaaaagt ggcttgtgtg 120
<210> 85
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 85
tcccaggaga atcagtgcgt atacagcgtg gtccagccac ccgacagcga gatgggcatt 60
ttaagaaacg ctctcggcca gatctccgaa ccagagccag aaggaatctg tacaaaaaac 120
<210> 86
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 86
aggagtcaga acaagcaggg gttgctaagg atgcaaaatc tgtggcctca gatgttcccc 60
tctacaagga tggggaggct aagactgacc cagcagggct gagcagtccc catctgccag 120
<210> 87
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 87
gcacatcctc tgcagcaccc gacctggagg gtcccgaatt cccagttgag tctgtggctt 60
ctcggatcca ggctgagcca gacaacttgg cacgtgcctc tgcatctcca gacagaattc 120
<210> 88
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 88
ctagcctgcc tcaggaaact gtggatcagg aacccaagga tcagaagagg aaatcctttg 60
agcaggcggc ctctgcatcc tttcccgaaa agaagccccg gcttgaagat cgtcagtcct 120
<210> 89
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 89
ttcgtaacac aattgaaagt gttcacaccg aaaagccaca gcccactaaa gaggagccca 60
aagtcccgcc catccgggta ggagactgtt tgattcctgg ctgccctgga gccaggtttt 120
<210> 90
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 90
agatcaccca gtcagttaaa actattttct aattcttttt ttgcagattc aactttcacg 60
tatcaaacca ccctgggtgg ttaaaggtca gcccacttac cagatatgcc cccggatcat 120
<210> 91
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 91
cataccatct gttgagcccc aggttggaga ggagtgggag aaagctgctc ccacccctcc 60
tgcattgcct ggggatttga cagctgagga gggtctagat cctcttgaca gccttacttc 120
<210> 92
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 92
actctggact gtgccatctc gaggaggcag tgacagcaat ggcagttact gtcaacaggt 60
ggacattgaa aagctgaaaa tcaacggaga ctctgaagca ctgagtcctc acggtgagtc 120
<210> 93
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 93
cacggataca gcctctgact ttgaaggtca cctcacggag gacagcagtg aggctgacac 60
tagagaagct gcagtgacaa agggatcttc ggtggacaag gatgagaaac ccaattggaa 120
<210> 94
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 94
ccaatctgcc ccactgtcca aggtgaatgg tgacatgcgt ctggttacaa ggacagatgg 60
gatggttgct cctcagagct gggtgtctcg agtatgtgcg gtccgccaaa agatcccaga 120
<210> 95
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 95
ttccctactg ctggccagta ctgagtacca gccaagagcc gtgtgcctgt ccatgcctgg 60
gtcctcagtg gaggccacta acccacttgt gatgcagttg ctgcagggta gcttgcccct 120
<210> 96
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 96
agagaaggtt cttccaccag cccacgatga cagcatgtca gaatccccac aagtaccact 60
tacaaaagac cagagccatg gctcgctacg catgggatct ttacatggtc ttggaaaaaa 120
<210> 97
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 97
cagtggcatg gttgatggaa gcagccccag ttctttaagg gctttgaagg agcctcttct 60
gccagatagc tgtgaaacag gcactggtct tgccaggatt gaggccaccc aggctcctgg 120
<210> 98
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 98
agcaccccaa aagaattgca aggcagtccc aagttttgac tccctccatc cagtgacaaa 60
tcccattaca tcctctagga aactggaaga aatggattcc aaagagcagt tctcttcctt 120
<210> 99
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 99
tagttgtgaa gatcagaagg aagtccgtgc tatgtcacag gacagtaatt caaatgctgc 60
tccaggaaag agcccaggag atcttactac ctcgagaaca cctcgtttct catctccaaa 120
<210> 100
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 100
tgtgatctcc tttggtccag agcagacagg tcgggccctg ggtgatcaga gcaatgttac 60
aggccaaggg aagaagcttt ttggctctgg gaatgtggct gcaacccttc agcgccccag 120
<210> 101
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 101
ccccaccacg gagtcctcct gccggggttg gactggcgcc aggaccctcg cagacattaa 60
agcccgtgct ctgcaggtcc gaggggcgag aggtcaccac tgccatagag aggcggccac 120
<210> 102
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 102
gcctgcggac ccgatgcctc ttcctgctga gatccctcca gtttttccca gtgggaagtt 60
gggaccaagc acaaactcca tgtctggtgg ggtacagact ccaagggaag actgggctcc 120
<210> 103
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 103
aaagccacat gcctttgttg gcagcgtcaa gaatgagaag acttttgtgg ggggtcctct 60
taaggcaaat gccgagaaca ggaaagctac tgggcatagt cccctggaac tggtgggtca 120
<210> 104
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 104
cttggaaggg atgccctttg tcatggactt gcccttctgg aaattacccc gagagccagg 60
gaaggggctc agtgagcctc tggagccttc ttctctcccc tcccaactca gcatcaagca 120
<210> 105
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 105
ggcattttat gggaagcttt ctaaactcca actgagttcc accagcttta attattcctc 60
tagctctccc acctttccca aaggccttgc tggaagtgtg gtgcagctga gccacaaagc 120
<210> 106
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 106
aaactttggt gcgagccaca gtgcatcact ttccttgcaa atgttcactg acagcagcac 60
ggtggaaagc atctcgctcc agtgtgcgtg cagcctgaaa gccatgatca tgtgccaagg 120
<210> 107
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 107
ctgcggtgcg ttctgtcacg atgactgtat tggaccctca aagctctgtg tattgtgcct 60
tgtggtgaga taataaatta tggccatggg aaacattgta tatttagtgt gtgtattttg 120
<210> 108
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 108
cactgccatc ggaggggggg gtggcccggg tggaggtggc ggcggggcca ccgatgaggg 60
aggtggcaga ggcagcagca gtggtgatgg tggtgaggcc tgtggccacc ctgagcccag 120
<210> 109
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 109
gggaggcccg agcacccctg gaaagtgtac gtcagatcta cagcgaacac aactactgcc 60
gccttatcct ctaaatgggg agcataccca ggccggaact gccatgtcca gagctaggag 120
<210> 110
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 110
agaggacctg ccttctctga gaaaggagga aagctgccta ctacagaggg ctacagttgg 60
actcacagat gggctaggag atgcctccca actccccgtt gctcccactg gggaccagcc 120
<210> 111
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 111
atgccaggcc ttgcccctac tgtcctccca aacctcagta gctgagagat tagtggagca 60
gcctcagttg catccggatg ttagaactga atgtgagtct ggcaccactt cctgggaaag 120
<210> 112
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 112
tgatgatgag gagcaaggac ccaccgttcc tgcagacaat ggtcccattc tgtctctagt 60
gggagatgat acattagaga aaggaactgg ccaagctctt gacagtcatc ccactatgaa 120
<210> 113
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 113
ggatcctgta aatgtgaccc ccagttccac acctgaatcc tcaccgactg attgcctgca 60
gaacagagca tttgatgacg aattagggct tggtggctca tgccctccta tgagggaaag 120
<210> 114
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 114
tgatactaga caagaaaact tgaaaaccaa ggctctcgtt tctaacagtt ctttgcattg 60
gatacccatc ccatcgaatg atgaggtagt gaaacagccc aaaccagaat ccagagaaca 120
<210> 115
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 115
tgcccaggaa gagccccagc catggaacac cagctcctgt gctgcgaagt ggaaaccatc 60
cgccgcgcgt accccgatgc caacctcctc aacgaccggg tgctgcgggc catgctgaag 120
<210> 116
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 116
gcggaggaga cctgcgcgcc ctcggtgtcc tacttcaaat gtgtgcagaa ggaggtcctg 60
ccgtccatgc ggaagatcgt cgccacctgg atgctggagg tgcggggctt cgggcggctc 120
<210> 117
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 117
aggccgccga ggaggaggaa gaggaggagg aggaggtgga cctggcttgc acacccaccg 60
acgtgcggga cgtggacatc tgagggcgcc aggcaggcgg gcgccaccgc cacccgcagc 120
<210> 118
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 118
gcacagcaga ggagacagtt catcagttgc tttcccccac agacagtcac aatattatgt 60
gatgtatttc agaggcgatg atattctcac catcctgact gaagtgaact atgaagtaag 120
<210> 119
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 119
caacaaggat gacaagaaaa acatggggaa acagatgcct cagcctactt tcacactaag 60
aaaaaaactt gtcttccctt ctgattgatg gtgctatttt gtttgttttg ttttgtttt 119
<210> 120
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 120
tggttactct ttacagctat ttgtttcctg atggacgaaa tcagaatcct gatctgactg 60
gcttatgtga accaactccc caagaccata tcaaagtgac ccaggtgagt tttgtttcac 120
<210> 121
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 121
tataattgca gttatttatt caactaatag tcttttaatt ttttttaatc aaaggaacaa 60
tatgaattat actgtgagat gggctccaca ttccaactat gtaaaatatg tgctgaaaat 120
<210> 122
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 122
cttttgcttc ttctgcagga atcagaaggt cagggctgtc ctttctgccg atgtgaaatt 60
aaaggtactg aacccatcgt ggtagatccg tttgatccta gagggagtgg cagcctgttg 120
<210> 123
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 123
ttctggttca gagcatgtga ttgcgaccag cagattgata gctgtacgta tgaagcaatg 60
tataatattc agtcccaggc gccatctatc accgagagca gcacctttgg taagttgcca 120
<210> 124
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 124
caggatgagg aagtgagctc gctcacttgt gatgccctga ctgagctgct ggctaagatc 60
accaacacag acgtggtagg tggccaccac cttcttggct aagtccagat gtctcttctc 120
<210> 125
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 125
cgtgacggat ccaagtcgga ggatgaactg gaccaagcca gcacccctac agacgtgcgg 60
gatatcgacc tgtgaggatg ccagttgggc cgaaagagag agacgcgtcc ataatctggt 120
<210> 126
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 126
gctacgtacc ccgcgcctcc tacttccagt gcgtgcagcg ggagatcaag ccgcacatgc 60
ggaagatgct ggcttactgg atgctggagg taccgcccgg acgcgtcccc ctcccccacc 120
<210> 127
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 127
gagtgtttgc tcctcactct tctcccatgt tcccaggact gcctgcgggc ctgtcaggag 60
cagatcgaag ctgcactcag ggagagcctc agggaagcct ctcagaccag ctccagccca 120
<210> 128
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 128
gcgcccaaag ccccccgggg ctccagcagc caagggccca gccagaccag cactcctaca 60
gatgtcacag ccatacacct gtagccctgg agaggccctc tggagtggcc actaagcaga 120
<210> 129
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 129
cacacttgga ggagtaagaa ctagggggtt taatattccc aaacaggtga gcagaagtgg 60
cctatatcgc tccccgtcga tgccagagaa cttgaacagg ccaagactga agcaggtgga 120
<210> 130
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 130
aaaattcaag gacaacacaa taccagataa agttaaaaaa aagtattttt ctggccaagg 60
aaagctcagg aaggtaagaa ctttttaaga tgaaaatctt gttcatcttc tatctcatgt 120
<210> 131
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 131
ggccaagaag tcggtggaca agaacagcaa cgagtaccgg gtgcggcgcg agcgcaacaa 60
catcgcggtg cgcaagagcc gcgacaaggc caagcagcgc aacgtggaga cgcagcagaa 120
<210> 132
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 132
ggtgctggag ctgaccagtg acaatgaccg cctgcgcaag cgggtggaac agctgagccg 60
cgaactggac acgctgcggg gcatcttccg ccagctgcca gagagctcct tggtcaaggc 120
<210> 133
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 133
catgggcaac tgcgcgtgag gcgcgcggct gtgggaccgc cctgggccag cctccggcgg 60
ggacccaggg agtggtttgg ggtcgccgga tctcgaggct tgcccgagcc gtgcgagcca 120
<210> 134
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 134
cgccgccttc ggctttcccc ggggcgcggg ccccgcgcag cctcccgccc cacctgccgc 60
cccggagccg ctgggcggca tctgcgagca cgagacgtcc atcgacatca gcgcctacat 120
<210> 135
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 135
cgacccggcc gccttcaacg acgagttcct ggccgacctg ttccagcaca gccggcagca 60
ggagaaggcc aaggcggccg tgggccccac gggcggcggc ggcggcggcg actttgacta 120
<210> 136
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 136
cccgggcgcg cccgcgggcc ccggcggcgc cgtcatgccc gggggagcgc acgggccccc 60
gcccggctac ggctgcgcgg ccgccggcta cctggacggc aggctggagc ccctgtacga 120
<210> 137
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 137
atagggagac cttctgacag ccccactcct tgcagtgaaa ctggagcccc ccatgctgcg 60
gaccatggac cccagccctg aagcggcccc tccccaggca ggctgcctac agctgtgctg 120
<210> 138
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 138
acgctgcatc cgctggcccc tgcctggcca ctggagcgac tggagcccca gcctggagct 60
gagaactacc gaacggggta aggaggtgat gagaggctgg gagcagccaa cagtatccag 120
<210> 139
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 139
ccctcttctt gttccatcca cagcccccac tgtcagactg gacacatggt ggcggcagag 60
gcagctggac cccaggacag tgcagctgtt ctggaaggtg actccctctg aaggccatcc 120
<210> 140
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 140
cagctgcacc ttccacctgc cttcagaagc ccaggaggtg gcccttgtgg cctataactc 60
agccgggacc tctcgtccca ctccggtggt cttctcagaa agcagaggta aagggggctg 120
<210> 141
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 141
tccaccattt cccaccctgt ctccaggccc agctctgacc agactccatg ccatggcccg 60
agaccctcac agcctctggg taggctggga gccccccaat ccatggcctc agggctatgt 120
<210> 142
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 142
gattgagtgg ggcctgggcc cccccagcgc gagcaatagc aacaagacct ggaggatgga 60
acagaatggg agagccacgg ggtttctgct gaagggtgag gctggcctgg aaagttgggc 120
<210> 143
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 143
ccccctgagc tggggaagag cccccttacc cactacacca tcttctggac caacgctcag 60
aaccagtcct tctgtgagtc tatcctcagt tcccccacag ccccagaagg cctggaaggg 120
<210> 144
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 144
ccacctcatg gctgccagcc aggctggggc caccaacagt acagtcctca ccctgatgac 60
cttgacccca ggtaagggga gacgggggct gggcagagat ggctgtttgg agggtacccc 120
<210> 145
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 145
gccctcactg cagaggggtc ggagctacac atcatcctgg gcctgttcgg cctcctgctg 60
ttgctcacct gcctctgtgg aactgcctgg ctctgttgca gccccaagtg agtccctttg 120
<210> 146
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 146
taaccccagc cccaagccag gaggacgact gtgtctttgg gccactgctc aacttccccc 60
tcctgcaggg gatccgggtc catgggatgg aggcgctggg gagcttctag ggcttcctgg 120
<210> 147
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 147
acagagagaa ctcgatgcca ccgcaacggt attggcgaac cggcaggatg aaagtgagca 60
gtccagaaag cggcttatcg aacagagccg ggagttcaag aagaacactc cagaggtgag 120
<210> 148
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 148
accgccgtgg agggggcctg tttgctggcc gagtggggtg atggctgttt tcctgttgtg 60
cagatcccgt accagctttg gatctcggac agcaactcca gctcaaagtg cagcgcctgc 120
<210> 149
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 149
acgatattga aacagagaac cagaaactta gggaaactct ggaagaatac aacaaggaat 60
ttgctgaagt gaaaaatcaa ggttggtgga aaatgcgttc tttgcttttt cttttcttc 119
<210> 150
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 150
gccgaaacca tagctcttga gaaggaacag aagttacaga atgactttgc agaaaaggag 60
aggtgagcat gacttccagg cacacacaga ctgacatagc atttgcccct gaacttcaca 120
<210> 151
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 151
gcttcgtcct cttcctttgc agaaagctgc aggagacaca gatgtccacc acctcaaagc 60
tggaggaagc tgagcataag gttcagagcc tacaaacagg tttgatactc tccttcctag 120
<210> 152
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 152
tgtgactaac ctgctctctc cctctcaccc tcctagggcc gacgagattg aaatgatcat 60
gacggacctt gaaagggcaa accaggtagg accctggacg cttttagtcc taaaacccac 120
<210> 153
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 153
tccttcagag ggcagaggtg gctcagagag aggcggagac cttaagggaa cagctctcat 60
cggccaatca ctccctccag ctggcctcac agatccagaa ggcaccagac gtggtgggta 120
<210> 154
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 154
ataaataaaa taacccctct gttgtgctct tgcagcattc tgaagtccat ggagtttgca 60
ccgtccgagg gcgctgggac acaggtacgt gtctcacctc aatggtcagc actagcatca 120
<210> 155
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 155
tgccccttct aggatgcggc caagcccctg gaggtgctgt tgctggagaa gaaccgctcg 60
ctgcagtccg agaacgccgc gctgcgcatc tccaacagcg acctgagcgg taggttggcc 120
<210> 156
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 156
cattctgttt gcccttcctt gtagggtcag ccaggaggaa agggaaagac cagcctgaaa 60
gtcggcgccc gggatctttg ccggcccccc ctccttctca gttgccccgc aacccggggg 120
<210> 157
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 157
agcaggcttc caatactaat ggtacacacc agttctcacc agcggggtta agtcaagact 60
ttttcagctc atccctggca agccccagcc tacccctggc ttctacagga aaatttgcac 120
<210> 158
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 158
taaactctct tctccagcgg cagctaatgc agtccttcta ctccaaggct atgcaggaag 60
ccggaagcac aagcatgatt ttttcaacag gtccatacag cacaaactcc atatcttccc 120
<210> 159
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 159
aaagtccatt acaacaaagc ccagatgtca atggcatggc cccatccccc agccagtcag 60
aaagtgctgg gagcgtctcc gagggcgagg agatggacac tgcagaaatc gcccggcagg 120
<210> 160
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 160
tcaaagagca gctgattaag cacaatatcg gacaacgtat tttcggacat tatgtgttgg 60
gactgtctca agggtccgtg agcgagattc tggcccggcc caagccatgg aataaactga 120
<210> 161
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 161
ctgttcgtgg caaggagcca tttcacaaga tgaaacagtt cctctccgat gagcagaaca 60
tcctggccct ccgtagcatc caaggcagac aaagaggtga gagactggcg ttgggtggcg 120
<210> 162
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 162
tccccacagg taacatcacc acccggatcc gagcctcgga gactggctct gatgaagcca 60
tcaagtccat cctagagcaa gccaagaggg agctccaagt gcagaaaact ggtacagctt 120
<210> 163
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 163
atgtccccag ctgaaggggg ccgccctgcc acactctcac ccctgtttct ccatgcagca 60
gagccggccc agccttcctc cgcatccggc agcgggaact ctgatgacgc catccgctcc 120
<210> 164
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 164
atcctgcagc aagcccgccg ggagatggag gcccagcagg ctgccctcga ccctgcctta 60
aagcaggcac cactgtccca gagtgacatc accatcctca cccccaagct tctgtccacc 120
<210> 165
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 165
tcgcccatgc ccaccgtgtc cagctaccca cctctcgcca tctccctgaa gaagccctcc 60
gcagctcctg aggccggtgc ctctgctctg ccgaaccccc cggccctcaa aaaggaggcc 120
<210> 166
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 166
caggacgccc ccgggctgga cccccaggga gcagccgatt gtgcacaagg ggtcctgaga 60
caggtgaaaa atgaggtggg ccgcagcggt gcctggaagg accactggtg gagcgcggtg 120
<210> 167
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 167
cagccggaga gaagaaatgc cgcctcctcc gaggaggcca aggccgaaga aacgggcggc 60
gggaaagaga agggcagcgg tggcagcgga ggtggcagcc agcctcgggc cgagcgcagt 120
<210> 168
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 168
cagctccagg gaccctcgtc gtcagagtac tggaaggagt ggcccagcgc tgagtcccca 60
tactcccaga gctcagagct gagtctgacc ggggccagcc gcagcgagac accacagaac 120
<210> 169
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 169
agccccctgc catcctcccc gatcgtgccc atgtccaagc ccaccaagcc ctcggtcccc 60
ccgctgaccc ccgagcagta cgaggtctac atgtaccagg aggtggacac catcgagctc 120
<210> 170
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 170
acccttcatc cggatgcagc tctggctgaa cggcgagcta ggccagggtg ttctacccgt 60
ccagggccag cagcaagggc caggtaatgg gggtcctgcc acaggagagg ggctgccccc 120
<210> 171
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 171
cacggtttct cgtgtgcttt aattacagaa agcactccaa agacctccgc cagctgcagc 60
cctgcccctg agtccccgat gagttccagt gagtcggtga agagcctgac cgagctggtc 120
<210> 172
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 172
cagcagccct gtccccccat cgaggcgagc aaggacagca agccaccaga gcccagtgac 60
ccgccagcat ccgactccca gcccacaacc ccgctgcctc tctccggaca ctcggccctc 120
<210> 173
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 173
agcatccaag aattagtagc catgtccccg gagctggaca cctacggcat aaccaagcgg 60
gtgaaggagg tgctgacgga caacaacctc ggtaggttct cctctcggct gctgagtcag 120
<210> 174
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 174
ctgtcttgct tctgttttct ctctaggcca gcgcttattt ggggagacca tcttagggct 60
cacccaaggc tctgtctctg acctccttgc ccgccccaaa ccctggcata agctcagtct 120
<210> 175
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 175
acagccaggg cgccagcccc cagccccagc accagctgaa gaaaccccgg gtggtgctgg 60
ctccggagga gaaggaggcg ctgaaacgag cgtatcagca aaagccatac ccgtcaccaa 120
<210> 176
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 176
aaaccatcga agacctcgcc acccagctca acctgaaaac cagcaccgtc atcaactggt 60
tccacaacta caggtacgac ggctggctca cagggagcgc cggtcggccc aggggaaggg 120
<210> 177
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 177
ccgcttgttg tcttgtaggt ctcggatccg cagagaactg ttcattgagg aaattcaggc 60
cgggagtcag ggccaggcgg gcgccagcga ctcaccctcg gcccgcagcg gccgggcggc 120
<210> 178
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 178
ccatgccctc cagcggcccc ggggacacca gcagctctgc tgcggagcgg gaggaggacc 60
gaaaggtgag cagcgggctc tgctggggac cctctggcct gggagaggga gagccctgtc 120
<210> 179
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 179
agagctatta cccaatgggg acttggagaa gcggagtgag ccccagccag aggaggggag 60
ccctgctggg gggcagaagg gcggggcccc agcagaggga gagggtgcag ctgagaccct 120
<210> 180
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 180
gtgaccactg tgtaatgatt tctgctcctt ggggctccag gacggccggg gctttggcat 60
tggggagctg gtgtggggga aactgcgggg cttctcctgg tggccaggcc gcattgtgtc 120
<210> 181
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 181
ttggtggatg acgggccgga gccgagcagc tgaaggcacc cgctgggtca tgtggttcgg 60
agacggcaaa ttctcagtgg taagttgtgg ggtttggcag tagcctgggg tgggggaagg 120
<210> 182
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 182
ccccaggtgt gtgttgagaa gctgatgccg ctgagctcgt tttgcagtgc gttccaccag 60
gccacgtaca acaagcagcc catgtaccgc aaagccatct acgaggtcct gcaggtgagt 120
<210> 183
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 183
agcctgtcct gacaacccca accctggcgt gtcaccctcc aggtggccag cagccgcgcg 60
gggaagctgt tcccggtgtg ccacgacagc gatgagagtg acactgccaa ggccgtggag 120
<210> 184
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 184
gtgcagaaca agcccatgat tgaatgggcc ctggggggct tccagccttc tggccctaag 60
ggcctggagc caccagaagg taaatgaggg cacccagctt tctgggaccc ctgcccgcca 120
<210> 185
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 185
cccctccctc tgctttccag acatctgcat ctcctgtggg agcctcaatg ttaccctgga 60
acaccccctc ttcgttggag gaatgtgcca aaactgcaag gtaggagcac acccacccag 120
<210> 186
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 186
ctagaactgc tttctggagt gtgcgtacca gtacgacgac gacggctacc agtcctactg 60
caccatctgc tgtgggggcc gtgaggtgct catgtgcgga aacaacaact gctgcaggtg 120
<210> 187
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 187
ctcctctgct cactgggtct ccttccaggt gcttttgcgt ggagtgtgtg gacctcttgg 60
tggggccggg ggctgcccag gcagccatta aggaagaccc ctggaactgc tacatgtgcg 120
<210> 188
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 188
gttgtgctca ctgcttagga ccctccaaag gtttacccac ctgtcccagc tgagaagagg 60
aagcccatcc gggtgctgtc tctctttgat ggaatcgcta caggtgaggg gtgcaggccc 120
<210> 189
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 189
ccagggagat ggctccaagt aacggtgctg tctgctggct ggtgcagggc tcctggtgct 60
gaaggacttg ggcattcagg tggaccgcta cattgcctcg gaggtgtgtg aggactccat 120
<210> 190
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 190
cacggtgggc atggtgcggc accaggggaa gatcatgtac gtcggggacg tccgcagcgt 60
cacacagaag catgtatgtc catgctgtgg ggcgcagccc gtcttcccct ccctgcacac 120
<210> 191
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 191
ctttatcctc ccagatccag gagtggggcc cattcgatct ggtgattggg ggcagtccct 60
gcaatgacct ctccatcgtc aaccctgctc gcaagggcct ctacggtagg taccatcctg 120
<210> 192
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 192
ctatgcagac agccccagct gatggctttc tcttccgacc tctcagaggg cactggccgg 60
ctcttctttg agttctaccg cctcctgcat gatgcgcggc ccaaggaggg agatgatcgc 120
<210> 193
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 193
cccttcttct ggctctttga gaatgtggtg gccatgggcg ttagtgacaa gagggacatc 60
tcgcgatttc tcgaggtata gccagcaacc ttggtttggc cagctcacta atggcttcta 120
<210> 194
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 194
cgtctcctgt tttgtagtcc aaccctgtga tgattgatgc caaagaagtg tcagctgcac 60
acagggcccg ctacttctgg ggtaaccttc ccggtatgaa caggttggtg aaagctcctg 120
<210> 195
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 195
ttacagtctc tcttctgcct cctaggccgt tggcatccac tgtgaatgat aagctggagc 60
tgcaggagtg tctggagcat ggcaggatag ccaaggtcag ctccagcgtc tagaacctct 120
<210> 196
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 196
ttcagcaaag tgaggaccat tactacgagg tcaaactcca taaagcaggg caaagaccag 60
cattttcctg tcttcatgaa tgagaaagag gacatcttat ggtgcactga aatggaaagg 120
<210> 197
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 197
cctggtctgg ccagcactca ccctgccctc tctgcctttt ctcccccagg gtatttggtt 60
tcccagtcca ctatactgac gtctccaaca tgagccgctt ggcgaggcag agactgctgg 120
<210> 198
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 198
gccggtcatg gagcgtgcca gtcatccgcc acctcttcgc tccgctgaag gagtattttg 60
cgtgtgtgta agggacatgg gggcaaactg aggtagcgac acaaagttaa acaaacaaac 120
<210> 199
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 199
aggatgaggc ggggtttcgc cgtggcgcgc atgcgtgcag caaagaatgg aggagtcgga 60
acccgaacgg aaggtaggag agaggcgaga ccccgcgcgt gtgggagcgg gcgtgaagag 120
<210> 200
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 200
ctgacctgat ctttgtggtg gcaggaggta gaatatgtga aggaggaggc caagatggtg 60
tacctgctcg agtgcctgca gaagacaccc ccgcctgtga gtgcagccag gccaggccgg 120
<210> 201
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 201
gagactctgt ccttctctct gcagtacacc ggattggccg caccgggcgc tcgggaaaca 60
caggcatcgc cactaccttc atcaacaaag cgtgtggtga gtctgttacc agcgccacct 120
<210> 202
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 202
tttctctctt tctttctgcc tcatgctctc tccaacaggc ttgcagccaa tttactggag 60
cagggatgac gtagcccagt ggctcaagtg ggctgaaaat gagttttctt taaggccaat 120
<210> 203
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 203
tgacagcaac acgtttgaaa tgaatggcaa agctctcctg ctgctgacca aagaggactt 60
tcgctatcga tctcctcatt caggtgagag tctggactct tggcatatgc ccaacttgga 120
<210> 204
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 204
gttggaaaaa catctttcca tttctcgatt tccctttcct ttttctttcc aggtgatgtg 60
ctctatgaac tccttcagca tattctgaag cagaggaaac ctcggattct tttttcacca 120
<210> 205
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 205
ttcttccacc ctggaaactc tatacacaca cagccggagg tcatactgca tcagaaccat 60
gaagaaggta ctggaagagg tttctctttt cttgcctgag gtttagacaa atccaggaag 120
<210> 206
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 206
tcacgacaaa tcaccggcct tctcctgacc ccgagcagcg gcccctccgg tcccccctgg 60
acaacatgat ccgccgcctc tccccggctg agagagctca gggacccagg ccgcaccagg 120
<210> 207
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 207
caacaagaaa cattttcaac agtgttttct tgcccttttc ctctgtagac tgtagactgc 60
tttgggatta cgtctatcag ttgctttctg acagccggta cgaaaacttc atccgatggg 120
<210> 208
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 208
aggacaaaga atccaaaata ttccggatag tggatcccaa cggactggct cgactgtggg 60
gaaaccataa ggtaaaaggg cagcagatat ctgctccata aactagtgcc aaaatgaagt 120
<210> 209
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 209
gaacaaacag aacagaacaa acatgaccta tgagaaaatg tccagagccc tgcgccacta 60
ctacaaacta aacattatca ggaaggagcc aggacaaagg cttttgttca ggtagcactt 120
<210> 210
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 210
agtgttcggt gaccagtgac ttggattttc caacacaagt catcccatta aagactctga 60
atgcagttgc ttcagtaccc ataatgtatt cttggtctcc cctacagcag aattttatgg 120
<210> 211
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 211
gttatttcac atttgatact tagagtaaac ctgcttttta aatatatttt tttcttttag 60
gtggaagatg aaactgtttt acataacatt ccttatatgg gagatgaagt tttagatcag 120
<210> 212
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 212
gatggtactt tcattgaaga actaataaaa aattatgatg ggaaagtaca cggggataga 60
ggtgagccat atgcttcttc tcttggaaaa aggggctaaa gaattgagag acacttttgt 120
<210> 213
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 213
tgatggagac gatcctgaag aaagagaaga aaagcagaaa gatctggagg atcaccgaga 60
tggtatgcct gattattagg attctgtgta gccattacag ctttctttca aaaacagtag 120
<210> 214
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 214
attgcttctc ctgtgtgttt ctgacatttg atgcgtttca gaatgtgggt ttataaatga 60
tgaaattttt gtggagttgg tgaatgccct tggtcaatat aatgatgatg acgatgatg 119
<210> 215
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 215
ctgtttagat aaagaaagcc gcccacctcg gaaatttcct tctgataaaa tttttgaagc 60
catttcctca atgtttccag ataagggcac agcagaagaa ctaaaggaaa agtaagaatt 120
<210> 216
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 216
tttacttaaa tttttcttgg ttaatgtcag atataaagaa ctcaccgaac agcagctccc 60
aggcgcactt cctcctgaat gtacccccaa catagatgga ccaaatgcta aatctgttca 120
<210> 217
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 217
gagagagcaa agcttacact cctttcatac gcttttctgt aggcgatgtt ttaaatatga 60
ctgcttccta catcgtaagt gcaattattg tacgttttca ttttctatct ttgttgtgga 120
<210> 218
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 218
catttatttt gcagcttttc atgcaacacc caacacttat aagcggaaga acacagaaac 60
agctctagac aacaaacctt gtggaccaca gtgttaccag catttggtaa gacttagtgc 120
<210> 219
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 219
ctcaatgttt agagtcctca ttggcactta ctatgacaat ttctgtgcca ttgctaggtt 60
aattgggacc aaaacatgta gacaggtaag atatccgata aaaatgttgt taaaagagct 120
<210> 220
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 220
gttcgtgccc ttgtgtgata gcacaaaatt tttgtgaaaa gttttgtcaa tgtagttcag 60
agtgtaagta tttgttgctt tgatgcaatt gcatgagaac taaataggtc tttggttagc 120
<210> 221
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 221
agtgaagaac ctccaaacct ctctctgaag gtcaaaaccg ctttccggga tgccgctgca 60
aagcacagtg caacaccaag cagtgcccgt gctacctggc tgtccgagag tgtgaccctg 120
<210> 222
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 222
tttattctct agcatctatt gctggcacca tctgacgtgg caggctgggg gatttttatc 60
aaagatcctg tgcagaaaaa tgaattcatc tcagaatact gtggagaggt aaggcactga 120
<210> 223
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 223
cctgtgtctt tctttttaga ttatttctca agatgaagct gacagaagag ggaaagtgta 60
tgataaatac atgtgcagct ttctgttcaa cttgaacaat ggtatgtttc agagctggga 120
<210> 224
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 224
gcttactttt ttctttttag attttgtggt ggatgcaacc cgcaagggta acaaaattcg 60
ttttgcaaat cattcggtaa atccaaactg ctatgcaaaa ggtaggtacc tttgacgtga 120
<210> 225
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 225
cagtgtgtct ctttgcagtt atgatggtta acggtgatca caggataggt atttttgcca 60
agagagccat ccagactggc gaagagctgt tttttgatta caggttggta aagtacattt 120
<210> 226
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 226
ccagcggaca tctccttcct gttgtttcag atacagccag gctgatgccc tgaagtatgt 60
cggcatcgaa agagaaatgg aaatcccttg acatctgcta cctcctcccc cctcctctga 120
<210> 227
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 227
acagaaaaag cagacagctc tgaaagagag gcactcatgt cagaactcaa gatgatgacc 60
cagctgggaa gccacgagaa tattgtgaac ctgctggggg cgtgcacact gtcaggtaac 120
<210> 228
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 228
cagaggctcc atggagttcc ctggcctggg gtccctgggg acctcagagc ccctccccca 60
gtttgtggat cctgctctgg tgtcctccac accagaatca ggggttttct tcccctctgg 120
<210> 229
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 229
gcctgagggc ttggatgcag cagcttcctc cactgccccg agcacagcca ccgctgcagc 60
tgcggcactg gcctactaca gggacgctga ggcctacaga cactccccag gtaactccat 120
<210> 230
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 230
aaagctgagc tgagcctagc tccctcttct cctctccacc ccaccagagg ccagggagtg 60
tgtgaactgc ggagcaacag ccactccact gtggcggagg gacaggacag gccactacct 120
<210> 231
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 231
ccggccatgg aggtggcgcc cgagcagccg cgctggatgg cgcacccggc cgtgctgaat 60
gcgcagcacc ccgactcaca ccacccgggc ctggcgcaca actacatgga acccgcgcag 120
<210> 232
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 232
ctgctgcctc cagacgaggt ggacgtcttc ttcaatcacc tcgactcgca gggcaacccc 60
tactatgcca accccgctca cgcgcgggcg cgcgtctcct acagccccgc gcacggtgag 120
<210> 233
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 233
ctttctgccc accctgatcc tctctctctt tgcccgcagc ccgcctgacc ggaggccaga 60
tgtgccgccc acacttgttg cacagcccgg gtttgccctg gctggacggg ggcaaagcag 120
<210> 234
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 234
ccctctctgc cgctgcggcc caccaccaca acccctggac cgtgagcccc ttctccaaga 60
cgccactgca cccctcagct gctggaggcc ctggaggccc actctctgtg tacccagggg 120
<210> 235
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 235
accctagcac cacgggggct gcgtctccag cctcatcttc cgcggggggt agtgcagccc 60
gaggagagga caaggacggc gtcaagtacc aggtgtcact gacggagagc atgaagatgg 120
<210> 236
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 236
aaagtggcag tcccctgcgc ccaggcctag ctactatggg cacccagcct gctacacacc 60
accccatccc cacctacccc tcctatgtgc cggcggctgc ccacgactac agcagcggac 120
<210> 237
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 237
tcttccaccc cggaggcttc ctggggggac cggcctccag cttcacccct aagcagcgca 60
gcaaggctcg ttcctgttca ggtaaaggca ggtgctgggg acttcgtgga agaggggagc 120
<210> 238
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 238
acttgccggt taagcaggcc cccgtgtctc tccctgttcc cctgcagaag gccgggagtg 60
tgtcaactgt ggggccacag ccacccctct ctggcggcgg gacggcaccg gccactacct 120
<210> 239
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 239
gtgcaatgcc tgtggcctct accacaagat gaatgggcag aaccgaccac tcatcaagcc 60
caagcgaaga ctggtaggag cgggcacagg tggctgggag ggggctgctg ggcaggagct 120
<210> 240
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 240
ggtcgaggtg ggcgtgggag tccagcctgc tgacgctgcc ttgccctccc agtcggccgc 60
cagaagagcc ggcacctgtt gtgcaaattg tcagacgaca accaccacct tatggcgccg 120
<210> 241
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 241
aaacgccaac ggggaccctg tctgcaacgc ctgtggcctc tactacaagc tgcacaatgt 60
gagtgcgccc cgccccggcc accccgcccc tcccagggga cctctgcgct ttgtgctgcc 120
<210> 242
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 242
ggccctctga aaactggtgg ttgcctctag gttaacaggc cactgaccat gaagaaggaa 60
gggatccaga ctcggaaccg gaagatgtcc aacaagtcca agaagagcaa gaaaggggcg 120
<210> 243
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 243
aagccattat ctgcaaaaat atcccccggc ttgtgagtgg atgggtaaaa cctatcatca 60
taggtcgtca tgcttatggg gatcaagtaa gtcatgttgg caataatgtg attttgcatg 120
<210> 244
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 244
ggcggctgca gtgggaccac tattatctct gtcctcacag agttcaagct gaagaagatg 60
tggaaaagtc ccaatggaac tatccggaac atcctggggg ggactgtctt ccgggagccc 120
<210> 245
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 245
atcatctgca aaaacatccc acgcctagtc cctggctgga ccaagcccat caccattggc 60
aggcacgccc atggcgacca ggtaggccag ggtggagagg ggatccactg acctgggcac 120
<210> 246
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 246
tcatcagaaa atgagatgaa ttggtttcat tcgaatgacg gtggaaacgt tctctactac 60
gaagtgatgg tgactgggaa tcttggaatc cagtggaggc ataaaccaaa tgtaagtggc 120
<210> 247
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 247
ggtggagctg caccgacttt gacaacatcc tcatgaccgt cacctgcttt gagaagtctg 60
aggtcagtca ggacactggc ttggaccagg ccatacagcc atgaggtgtc cagtgtagcc 120
<210> 248
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 248
taatttctgg tcatttcccg aatagcaggt gcagggtgcc cagaagcagt tcaagaactt 60
tcagatcgag gtgcagaagg gccgctacag tctgcacggt tcggaccgca gcttccccag 120
<210> 249
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 249
ccacagaaat ctccaacctg ctggtggcta ctaagaaagc ccaggagtgg cagcccgtct 60
accccatgag ccagctgagt ttcgatcgga tcctcaagaa ggatctggtg caggtgagtc 120
<210> 250
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 250
ggaacttctg aagagaagaa gataaaagtg atcctcaaag tcttagaccc cagccacagg 60
gatatttccc tggcaagtgt cttcctctga actcctcccc tcccctctcc cgtttctgga 120
<210> 251
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 251
tcctgctcgc ccacaggcct tcttcgaggc agccagcatg atgagacagg tctcccacaa 60
acacatcgtg tacctctatg gcgtctgtgt ccgcgacgtg gagagtaagt gtgttctctt 120
<210> 252
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 252
aggagaggac cgtgccacag accaggttcc agacatggct atttttatgc ctacagatat 60
catggtggaa gagtttgtgg aagggggtcc tctggatctc ttcatgcacc ggaaaagcga 120
<210> 253
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 253
tgtccttacc acaccatgga aattcaaagt tgccaaacag ctggccagtg ccctgagcta 60
cttggtgaga atgtcctgat gtccataatg gtcccaccag aaaccagagt gtcctgggag 120
<210> 254
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 254
atagaatgcg ggaaggagca gcttggctaa acttgacctt tttactctgc aggaggataa 60
agacctggtc catggaaatg tgtgtactaa aaacctcctc ctggcccgtg agggcatcga 120
<210> 255
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 255
cagtgagtgt ggcccattca tcaagctcag tgaccccggc atccccatta cggtgctgtc 60
taggcaaggt gtgtctcccc tctcttccct ctggccccac cccgagtcag gggcaagtca 120
<210> 256
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 256
gtggagcttt ggaaccacgc tctgggaaat ctgctacaat ggcgagatcc ccttgaaaga 60
caagacgctg attgaggtga gcaggtgttt cccggggcgt gaggcaagtg agggctttca 120
<210> 257
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 257
ctcttctgtt agatccagat attgtttcag aaaaaaaacc agcaactgaa gtggacccca 60
cacattttga aaagcgcttc ctaaagagga tccgtgactt gggagaggta agttacaccc 120
<210> 258
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 258
cagcacatgc atctttccct ccagggccac tttgggaagg ttgagctctg caggtatgac 60
cccgaagggg acaatacagg ggagcaggtg gctgttaaat ctctgaagcc tgagagtgga 120
<210> 259
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 259
ctctcccatg gttccctcat ttggcagcct cagactgatg gatgtttttt ctaaacagga 60
ggaaatggta ttaagctcat catggaattt ctgccttcgg gaagccttaa ggaatatctt 120
<210> 260
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 260
aattggagac ttcggtttaa ccaaagcaat tgaaaccgat aaggagtatt acaccgtcaa 60
ggatgaccgg gacagccctg tgttttggta actgaatcaa accactaggt tttatcaatg 120
<210> 261
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 261
tttttttttt ccttagtctt tctttgaagc agcaagtatg atgagcaagc tttctcacaa 60
gcatttggtt ttaaattatg gagtatgtgt ctgtggagac gagagtaagt aaaactacag 120
<210> 262
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 262
ccagtacttg tggactgata tttgaatata tgtgcgttta actctaatag gaagaaaaca 60
cccttattca tgggaatgta tgtgccaaaa atattctgct tatcagagaa gaagacagga 120
<210> 263
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 263
catcctaatg tgatgtgtca tttagggtaa ttttgggagt gtggagatgt gccggtatga 60
ccctctacag gacaacactg gggaggtggt cgctgtaaaa aagcttcagc atagtactga 120
<210> 264
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 264
cccccgagcc acatcttctc cgtggaggat gccagcaccc aagtcctgct gtacaggatt 60
cggtaggaag tgccccccag cccccaggga ttgtacaatt ttatcatctc cttgcattt 119
<210> 265
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 265
ttgaagttgg gtggcctcag ctgatgctcc ctgtggcggc cccccagcac cgcagtgacc 60
tggtgagtgg gcgcctcccc gtgggcctca gtctcaagga gcagggtgag tgtctcagcc 120
<210> 266
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 266
tggccgtgtt ggacctggcc cggatggcgc gagagcaggc ccagcggccg ggagagctgc 60
tgaagactgt caggtgagag ccaccaggct gtggggacgg cctctgcttg ggagtgagca 120
<210> 267
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 267
cttggggact tttcacctct gatttctggt ttttctccct catcctctcc ccatagaaaa 60
gtccaacctg atcgtggtcc agagaggtca cagcccaccc acatcatcct tggttcagcc 120
<210> 268
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 268
ccaatcccaa taccagctga gtcagatgac atttcacaag atccctgctg acagcctgga 60
gtgggtaaga ggccctggga aatgaggcga tacctcagtc tggggtccag agactcagat 120
<210> 269
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 269
ccttcccacc ttccccagtc attcctggaa gcagcgagct tgatgagcca agtgtcgtac 60
cggcatctcg tgctgctcca cggcgtgtgc atggctggag acagtgagag ccccccaccc 120
<210> 270
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 270
gactcttcat tcatcaaacc ctcccgggca ttttcctgtg tctggccccc ttaggaggac 60
aaaggcctgc cccatggcaa tgtctctgcc cggaaggtgc tcctggctcg ggagggggct 120
<210> 271
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 271
gatgggagcc cgcccttcat caagctgagt gaccctgggg tcagccccgc tgtgttaagc 60
ctggagagta agttcctgga ggtggaggag ggaggggctg agcagggcaa ggaagtggat 120
<210> 272
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 272
gtggggcttc ggcgccacgg tctgggaagt gtttagtggc gtcaccatgc ccatcagtgc 60
cctggatcct gctaaggtca gagcccctca cccggcatcg gtctccgaac ccccactttg 120
<210> 273
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 273
ggacatcagt cccgctatcc cccagggcaa ctttggcagc gtggagctgt gccgctatga 60
cccgctaggc gacaatacag gtgccctggt ggccgtgaaa cagctgcagc acagcgggcc 120
<210> 274
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 274
atcctctctg cacgctcaca ccgcccgccc gcagggcatg gagtacctgg gctcccgccg 60
ctgcgtgcac cgcgacctgg ccgcccgaaa catcctcgtg gagagcgagg cacacgtcaa 120
<210> 275
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 275
gatcgctgac ttcggcctag ctaagctgct gccgcttgac aaagactact acgtggtccg 60
cgagccaggc cagagcccca ttttctggtg gggaacccgc gcctaggctc cgcccctatt 120
<210> 276
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 276
agcaaaacca gaaatcctga cttacgacag gctcgtgaat ggcatgctcc aatgtgtggc 60
agcaggattc ccagagccca caatagattg gtatttttgt ccaggaactg agcagaggtg 120
<210> 277
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 277
ggcttttgtt ttcttccctt tagatgctct gcttctgtac tgccagtgga tgtgcagaca 60
ctaaactcat ctgggccacc gtttggaaag ctagtggttc agagttctat agattctagt 120
<210> 278
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 278
gcattcaagc acaatggcac ggttgaatgt aaggcttaca acgatgtggg caagacttct 60
gcctatttta actttgcatt taaaggtaac aacaaaggta tatttctttt taatccaatt 120
<210> 279
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 279
ttgtagagca aatccatccc cacaccctgt tcactccttt gctgattggt ttcgtaatcg 60
tagctggcat gatgtgcatt attgtgatga ttctgaccta caaatattta caggtaacca 120
<210> 280
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 280
aatgactgag acaataatta ttaaaaggtg atctattttt ccctttctcc ccacagaaac 60
ccatgtatga agtacagtgg aaggttgttg aggagataaa tggaaacaat tatgtttaca 120
<210> 281
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 281
tagacccaac acaacttcct tatgatcaca aatgggagtt tcccagaaac aggctgagtt 60
ttggtcagta tgaaacaggg gctttccatg tcaccttttt gggtacacat aacagtgact 120
<210> 282
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 282
ttagcgagtg cccatttgac agaacgggaa gccctcatgt ctgaactcaa agtcctgagt 60
taccttggta atcacatgaa tattgtgaat ctacttggag cctgcaccat tggaggtaaa 120
<210> 283
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 283
ttgtgatttt ggtctagcca gagacatcaa gaatgattct aattatgtgg ttaaaggaaa 60
cgtgagtacc cattctctgc ttgacagtcc tgcaaaggat ttttagtttc aactttcgat 120
<210> 284
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 284
attaacctta tgtgtgacat gttctaatat agtcacattt tcattatttt tattataagg 60
cctgctgaaa atgactgaat ataaacttgt ggtagttgga gctggtggcg taggcaagag 120
<210> 285
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 285
aataatccag actgtgtttc tcccttctca ggattcctac aggaagcaag tagtaattga 60
tggagaaacc tgtctcttgg atattctcga cacagcaggt caagaggagt acagtgcaat 120
<210> 286
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 286
gagggaccag tacatgagga ctggggaggg ctttctttgt gtatttgcca taaataatac 60
taaatcattt gaagatattc accattatag gtgggtttaa attgaatata ataagctgac 120
<210> 287
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 287
gttactaatg actgtgctat aacttttttt tctttcccag agaacaaatt aaaagagtta 60
aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg ccttctagaa 120
<210> 288
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 288
cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct tttattgaaa 60
catcagcaaa gacaagacag gtaagtaaca ctgaaataaa tacagatctg ttttctgcaa 120
<210> 289
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 289
gggggtgtcg gcgttggagt tgtgaattcg ctgcgtttcc atgaaatcct gcggagtgtc 60
gctcgctacc gccgccgctg ccgccgccgc tttcggtgat gaggaaaaga aaatggcggc 120
<210> 290
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 290
aacgagtaaa ctgtgtctgt ctccacagcc gcctctttgg gttcgtgaga tttcatgaag 60
atggcgccag gacgaaggcc ctactgggca aggtaaggca gctgcgagtc ggagcgcgga 120
<210> 291
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 291
gacataatta tgactcataa ttattttcct ttcagcatta tctgcatacc agaggtacta 60
cagtttacag tctgactact ggaaggttag tgtacatttg catgctgatt tcatgtttgt 120
<210> 292
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 292
atgaatcctg caaccagcct caggatgcca ttaaatgcta cttaaatgca actagaagca 60
aaagttgtag taatacctct gcacttgcag cacgaattaa gtatttacag gtaaaatttt 120
<210> 293
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 293
ttcttctagc atttggaaca gctccgcgca aatagaaata atttaaatcc agcacagaaa 60
ctgatgctgg aacagctgga aagtcagttt gtcttaatgc aacaacacca agtgtgtata 120
<210> 294
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 294
attacacaac cagcatttac ttttcctttg tttttttgac agatgagacc aacaggagtt 60
gcacaggtac gatctactgg aattcctaat gggccaacag ctgactcatc actgcctaca 120
<210> 295
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 295
attaccttaa ccaaagagag caagccttca ggaaacatat tgacggtgcc tgaaacaagc 60
aggcacactg gagagacacc taacagcact gccagtgtcg agggacttcc taatcatgtc 120
<210> 296
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 296
catcagatga cggcagatgc tgtttgcagt cctagccatg gagattctaa gtcaccaggt 60
ttactaagtt cagacaatcc tcagctctct gccttgttga tgggaaaagc caataacaat 120
<210> 297
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 297
aatactgtgt ctctttttta agttctggga ggaggaggaa aggacccttt aaaaccataa 60
agtttgggac caatattgac ctatctgatg acaaaaagta agtccttgta gtaatatttc 120
<210> 298
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 298
ggtatttgta gcagagtttc acttttgatg tgttatttta ttgcaggtgg aagttgcagc 60
tacatgagct gactaaactt cctgcttttg tgcgtgtcgt atcagcagga aatcttctaa 120
<210> 299
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 299
aggtcatcag gaaaataaca acttctgttc agttaacata aatattggcc caggtgactg 60
tgaatggttt gttgttcctg aaggttactg gggtgttctg aatgacttct gtgaaaagta 120
<210> 300
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 300
tttgcattat ttttttctta tttcagaaat aatttgaatt tcctaatggg ttcttggtgg 60
cccaatcttg aagatcttta tgaagcaaat gttccagtgt ataggtttat tcagcgacct 120
<210> 301
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 301
ggagatttgg tctggataaa tgcaggcact gttcattggg ttcaggctat tggctggtgc 60
aacaacattg cttggaatgt tggtccactt acaggtatta taaagaatat gctttaaaaa 120
<210> 302
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 302
atggttcatc tttcctggaa tatggcacga aatatcaagg tctcagatcc aaagcttttt 60
gaaatgatta agtaagtctt ttctaaaact gctgtagtcc ctctcttttt ggggagtgtt 120
<210> 303
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 303
aacaagcgga aacttggaaa actttgtggt gctagaacag tacaaaatgg aggacctgat 60
gcaagtctat gaccaattta cattagtaag tcaaatcaac atgtgagtac atagttagct 120
<210> 304
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 304
ttatacataa aattacccaa gttgcaaata tatgtcttcc acccttgact ctcaggatag 60
tgcagcagga tgcagcggaa cccccccgat ttgccgacaa gcccagacca aactagaagt 120
<210> 305
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 305
tctctcctac ctaagtgcgt gccgccaccc gatggaagat tcgatggaca tggacatgag 60
ccccctgagg ccccagaact atcttttcgg taactgctgg ggggagctgg agcgaggccg 120
<210> 306
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 306
gcttttttct tcatttacag gttgtgaact aaaggccgac aaagattatc actttaaggt 60
ggataatgat gaaaatgagc accagttatc tttaagaacg gtacttaaac tttcaaaata 120
<210> 307
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 307
gggggggtgt aaaataggtg gaactcaaaa gttgaagtag tatttttttt ttgttcacag 60
gtcagtttag gggctggtgc aaaggatgag ttgcacattg ttgaagcaga ggcaatgaat 120
<210> 308
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 308
tacgaaggca gtccaattaa agtaacactg gcaactttga aaatgtctgt acagccaacg 60
gtaagggcac ttacatactt tggatgttgt gtcaaggttt aattctgttt taaggtaggt 120
<210> 309
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 309
ttctcccttc taggtttccc ttgggggctt tgaaataaca ccaccagtgg tcttaaggtt 60
gaagtgtggt tcagggccag tgcatattag tggacagcac ttagtaggta tgttattttt 120
<210> 310
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 310
ttttagctgt ggaggaagat gcagagtcag aagatgaaga ggaggaggat gtgaaactct 60
taagtatatc tggaaagcgg tctgcccctg gaggtggtag caaggttcca caggtagaga 120
<210> 311
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 311
tttattatag gttttgtatt atagctttta gtttggtgat agaacagctc ttgttcatga 60
gtacgtatct tttcttttaa aagaaaaaag taaaacttgc tgctgatgaa gatgatgac 119
<210> 312
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 312
gaagaggatg atgatgaaga gtaagtatga ttttagaaac ttgatatact tccggaatct 60
tgacaaaaaa aggaatttga catagttata tgcatgaggg ttttataaaa gtcatttaca 120
<210> 313
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 313
gatgatgagg aagctgaaga aaaagcgcca gtgaagaaag tgagtagata caatgctaca 60
aggttgttaa actaacaata gaaatggtga ttttttagtg ctatttgctt gttttgtagt 120
<210> 314
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 314
aaatttctaa ttgcagtcta tacgagatac tccagccaaa aatgcacaaa agtcaaatca 60
gaatggaaaa gactcaaaac catcatcaac accaagatca aaagtaagtg gctacattta 120
<210> 315
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 315
ctttttcagg gacaagaatc cttcaagaaa caggaaaaaa ctcctaaaac accaaaagga 60
cctagttctg tagaagacat taaagcaaaa atgcaagcaa gtatagaaaa agtgagtaaa 120
<210> 316
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 316
aataatgctt ttgtcttaat agggtggttc tcttcccaaa gtggaagcca aattcatcaa 60
ttatgtgaag aattgcttcc ggatgactga ccaagaggta actggatttt ctggggacat 120
<210> 317
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 317
ttgtggttcc ttaaccacat ttcttttttt ttttttccag gctattcaag atctctggca 60
gtggaggaag tctctttaag aaaatagttt aaacaatttg ttaaaaaatt ttccgtctta 120
<210> 318
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 318
tgaaatgact gagtacaaac tggtggtggt tggagcaggt ggtgttggga aaagcgcact 60
gacaatccag ctaatccaga accactttgt agatgaatat gatcccacca tagaggtgag 120
<210> 319
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 319
tccctgcccc cttaccctcc acacccccag gattcttaca gaaaacaagt ggttatagat 60
ggtgaaacct gtttgttgga catactggat acagctggac aagaagagta cagtgccatg 120
<210> 320
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 320
agagaccaat acatgaggac aggcgaaggc ttcctctgtg tatttgccat caataatagc 60
aagtcatttg cggatattaa cctctacagg tactaggagc attattttct ctgaaaggat 120
<210> 321
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 321
cagttgatac aaaacaagcc cacgaactgg ccaagagtta cgggattcca ttcattgaaa 60
cctcagccaa gaccagacag gtatggtaca gctttcagca tttgtgcaag agtttgcatc 120
<210> 322
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 322
ttcagccaca ggctcccaga catgacagcc atcatcaaag agatcgttag cagaaacaaa 60
aggagatatc aagaggatgg attcgactta gacttgacct gtatccattt ctgcggctgc 120
<210> 323
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 323
atggaacatt ttttttcaat ttggcttctc ttttttttct gtccaccagg gagtaactat 60
tcccagtcag aggcgctatg tgtattatta tagctacctg ttaaagaatc atctggatta 120
<210> 324
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 324
ctgtgaaata atactggtat gtatttaacc atgcagatcc tcagtttgtg gtctgccagc 60
taaaggtgaa gatatattcc tccaattcag gacccacacg acgggaagac aagttcatgt 120
<210> 325
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 325
actttgagtt ccctcagccg ttacctgtgt gtggtgatat caaagtagag ttcttccaca 60
aacagaacaa gatgctaaaa aaggtttgta ctttactttc attgggagaa atatccaaaa 120
<210> 326
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 326
atggcagttt tttggcaagg cctagtaaaa gtaaccctgg agacttcaca ctttccgtta 60
ggtaagttgg aatgaaaaga gaggatcctg agagtgtttt ctaggtagga agtggtaaaa 120
<210> 327
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 327
cctttccaat ggactatttt agaagaaatg gagctgtcac ccacatcaag attcagaaca 60
ctggtgatta ctatgacctg tatggagggg agaaatttgc cactttggct gagttggtcc 120
<210> 328
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 328
ttttttattt tttaaaaact ttaggtggtt tcatggacat ctctctggga aagaagcaga 60
gaaattatta actgaaaaag gaaaacatgg tagttttctt gtacgagaga gccagagcca 120
<210> 329
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 329
ggaactgaaa tacgacgttg gtggaggaga acggtttgat tctttgacag atcttgtgga 60
acattataag aagaatccta tggtggaaac attgggtaca gtactacaac tcaagcaggt 120
<210> 330
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 330
tttctttcca gacactacaa caacaggagt gcaaacttct ctacagccga aaagagggtc 60
aaaggcaaga aaacaaaaac aaaaatagat ataaaaacat cctgccctgt aagtatcaat 120
<210> 331
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 331
gtggtctctt tttcttctag ttgatcatac cagggttgtc ctacacgatg gtgatcccaa 60
tgagcctgtt tcagattaca tcaatgcaaa tatcatcatg gtaagctttg cttttcacag 120
<210> 332
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 332
cctgcttttt gtccttctgc ccgcagtgct ggaattggcc ggacagggac gttcattgtg 60
attgatattc ttattgacat catcagagag aaaggtgggt catctggtgg gcaagaagcg 120
<210> 333
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 333
ggctctgcag tttctcttta ttcttcatga tgtttccttc gtaggtgttg actgcgatat 60
tgacgttccc aaaaccatcc agatggtgcg gtctcagagg tcagggatgg tccagacaga 120
<210> 334
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 334
agcacagtac cgatttatct atatggcggt ccagcattat attgaaacac tacagcgcag 60
gattgaagaa gagcaggtac cagcctgagg gctggcatgc ggattctcat tctcttgcta 120
<210> 335
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 335
tcagaaaagc aagaggaaag ggcacgaata tacaaatatt aagtattctc tagcggacca 60
gacgagtgga gatcagagcc ctctcccgcc ttgtactcca acgccaccct gtgcagagta 120
<210> 336
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 336
gaacagagca ccagcaatct gaatgagaaa attaaccatt tagaatatga agatcaatat 60
aaggatgata attttggaga aggaaatgat ggtggaatat taggtatgtg ggaatgatgg 120
<210> 337
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 337
tcctaactca atcggcttgt tgtgatgcgt atccccgtag atgccagcac gagccgccgc 60
ttcacgccgc cttccaccgc gctgagccca ggcaagatga gcgaggcgtt gccgctgggc 120
<210> 338
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 338
gccccggacg ccggcgctgc cctggccggc aagctgagga gcggcgaccg cagcatggtg 60
gaggtgctgg ccgaccaccc gggcgagctg gtgcgcaccg acagccccaa cttcctctgc 120
<210> 339
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 339
tccgtgctgc ctacgcactg gcgctgcaac aagaccctgc ccatcgcttt caaggtactg 60
gcccggagag ggtggtggga ggacaggcgg aggccggcga ggccggggga gatccgcgag 120
<210> 340
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 340
aacatccctg atgtctgcat ttgtcctttg actggtgttt aggtggtggc cctaggggat 60
gttccagatg gcactctggt cactgtgatg gctggcaatg atgaaaacta ctcggctgag 120
<210> 341
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 341
ctgagaaatg ctaccgcagc catgaagaac caggttgcaa gatttaatga cctcaggttt 60
gtcggtcgaa gtggaagagg tacgttatct gtcaaaacta tgcttgaaac acgtttcatg 120
<210> 342
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 342
ccccacaggg aaaagcttca ctctgaccat cactgtcttc acaaacccac cgcaagtcgc 60
cacctaccac agagccatca aaatcacagt ggatgggccc cgagaacctc gaagtaagtg 120
<210> 343
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 343
tcccctccct gctccccaca ataggacatc ggcagaaact agatgatcag accaagcccg 60
ggagcttgtc cttttccgag cggctcagtg aactggagca gctgcggcgc acagccatga 120
<210> 344
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 344
tgcacccagc aacgcccatt tcacctggac gtgccagcgg catgacaacc ctctctgcag 60
aactttccag tcgactctca agtaagccac ttgaaaacac attctttgca gctgagctgg 120
<210> 345
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 345
agcggcaccc gacctgacag cgttcagcga cccgcgccag ttccccgcgc tgccctccat 60
ctccgacccc cgcatgcact atccaggcgc cttcacctac tccccgacgc cggtcacctc 120
<210> 346
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 346
cttttctctt tttcagatcc tcgtggtcat tgaaccgcta ttgattgatg aagattacta 60
tgctagagtg gaaggccgag agatcatttc taatttggca aaggtattta catttaattt 120
<210> 347
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 347
aatattacca actcatgact gtcctttctt tgtttacatt ttaggctgct ggtctggcta 60
ctatgatctc taccatgaga cctgatatag ataacatgga tgagtatgtc cgtaacacaa 120
<210> 348
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 348
cagctagagc ttttgctgtt gtagcctctg ccctgggcat tccttcttta ttgcccttct 60
taaaagctgt gtgcaaaagc aagaagtcct ggcaagcgag acacactggt attaagattg 120
<210> 349
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 349
tacaacagat agctattctt atgggctgtg ccatcttgcc acatcttaga agtttagttg 60
aaatcattga acatggtaag ttgtaatgta actttgtctt tttttttttt tctttaggag 120
<210> 350
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 350
cttaggtaat gttggggcat agttaaaacc tgtgtttggt tttgtaggtc ttgtggatga 60
gcagcagaaa gttcggacca tcagtgcttt ggccattgct gccttggctg aagcagcaac 120
<210> 351
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 351
tccttatggt atcgaatctt ttgattctgt gttaaagcct ttatggaagg gtatccgcca 60
acacagagga aaggtaaatc caccaattac cttttgattt atcttcatta aagttaaggc 120
<210> 352
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 352
ataaatctaa attactaaag tacatatatt ttttatttaa aaatagggtt tggctgcttt 60
cttgaaggct attgggtatc ttattcctct tatggatgca gaatatgcca actactatac 120
<210> 353
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 353
tagagaagtg atgttaatcc ttattcgaga attccagtct cctgatgagg aaatgaaaaa 60
aattgtgctg aaggtaatta ttccagattt gttaatgtaa actggatatg tttcatggtt 120
<210> 354
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 354
gagacaattg agaaaattat gggtaatttg ggagcagcag atattgatca taaacttgaa 60
gaacaactga ttgatggtat tctttatgct ttccaagaac agactacaga ggtaatgaaa 120
<210> 355
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 355
cgagtcccac agtgaggaga cgatccccag cgacagcggc attgggacag acaacaacag 60
cacttctgac caagcggaga agagctcaga atcccgaagg aggtactctt ttgatttctg 120
<210> 356
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 356
gaaccacatg gactggtcca ccaactctga cagcggaccc gtcactcaga attgcttcat 60
cagtccagag tctggcagag aaactgcaag caccagcaag atccccgctc ttgagcccgt 120
<210> 357
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 357
cagccgcagc cgcctccgaa gatgggggat ttctacgacc cggagcaccc gacccctgag 60
taagtatcag ctccgcggcc ccccgctgct gtcggccgcc ttatccccag ccgagggcgg 120
<210> 358
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 358
ccgccgccgc cgccgccgcc gcggtcggac ctgcggcctc ctcccctccc ctcccccgcg 60
tcgccctgct gcggggaggg ggctcgcgtc gccgtctcca gccgctcccg atgaagcag 119
<210> 359
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 359
tttatgattt ctttttattc ttttgcatgt tttcttggct gaatggaaat agagaagaag 60
aaaatgaggt aagtataatt taactagtca ttcaatgatt atgagatgga ccactaaaca 120
<210> 360
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 360
cttaccagta gatgtagcag ttagatctct gaaagaacca ccaattataa ttgtaccaga 60
atctttagaa gcagatacta agcaggacac tatatctaat agtttagaag aacacgtaac 120
<210> 361
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 361
tagtttacag acaccttgtt caaaaggtag tgaattaaga atgattaata aaaatcctga 60
aagagaaaag gctgggtctc cagctccatc aaatcgatta aatgattcac ctactttaaa 120
<210> 362
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 362
aaagctagat gaattgccta tttttaagtc cgaatttata acacatgata gccatgatag 60
tattaaggaa ttagactctt tatctaaagt gaagaatgat caattaagaa gtttttgtcc 120
<210> 363
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 363
catagaatta aatataaatg gatctcctgg ggcagaatct gatttggcaa cattttgcac 60
ttctaaaact gatgctgttt taatgacttc tgatgatagt gtgactggat cggaattatc 120
<210> 364
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 364
ccctttggtc aaagcatgca tgctttcatc aaatggattt cagaatatta gtaggtgcaa 60
agaaaaagac ttggatgata cctgcatgct gcataagaag tcagaaagcc catttagaga 120
<210> 365
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 365
aacagaacct ctggtgtcac cacaccaaga taaactcatg tctatgccag ttatgactgt 60
ggattattcc aaaacagtag ttaaagaacc agttgatacg agggtttctt gctgcaaaac 120
<210> 366
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 366
caaagattca gacatatact gtactttgaa cgatagcaac ccttctttgt gtaactctga 60
agctgaaaat attgagcctt cagttatgaa gatttcttca aatagcttta tgaatgtgca 120
<210> 367
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 367
tttggaatca aaaccagtta tatgtgatag tagaaatttg acagatcact caaaatttgc 60
atgtgaagaa tataagcaga gcatcggtag cactagttca gcttctgtta atcattttga 120
<210> 368
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 368
tgatttatat caacctattg ggagttcagg tattgcttca tctcttcaga gtcttccacc 60
aggaataaag gtggacagtc taactctctt gaaatgcgga gagaacacat ctccagttct 120
<210> 369
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 369
ggatgcagtg ctaaagagta aaaaaagttc agagttttta aagcatgcag ggaaagaaac 60
aatagtagaa gtaggtagtg accttcctga ttcaggaaag ggatttgctt ccagggagaa 120
<210> 370
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 370
caggcgtaat aatgggttat ctgggaaatg tttgcaagag gctcaagaag aagggaattc 60
catattgcct gaaagaagag gaagaccaga aatctcttta gatgaaagag gagaaggagg 120
<210> 371
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 371
tcaaatattg aatgagcaag cagatatttc ctcaaaaaaa gaagattccc atattgggaa 60
ggatgaagaa attccagata gttctaagat tagtctgagc tgtaaaaaaa caggttctaa 120
<210> 372
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 372
acatgtgcat acttctgatg actcagaagt tgtattttct tcttgtgatt tgaatttaac 60
catggaagac agtgatggtg taacttatgc attaaagtgt gacagtagtg gtcatgcccc 120
<210> 373
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 373
agaaattgtg tctacagttc atgaagatta ttctggctct tctgaaagtt caaatgatga 60
aagtgattca gaagatacag attcggatga tagcagtatt ccaagaaacc gtctccagtc 120
<210> 374
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 374
tgttgtggtt gtgccaaaga attctacttt gcccatggaa gaaacaagtc cttgttcttc 60
tcggagcagt caaagttata gacactattc tgaccattgg gaagatgaga gattggagtc 120
<210> 375
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 375
aaggagacat ttgtatgagg aaaaatttga aagtatagca agtaaagcct gtcctcaaac 60
tgataagttt ttccttcata aaggaacaga gaagaatccg gaaatttctt ttacacagtc 120
<210> 376
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 376
cagtagaaaa caaatagata atcgcctgcc tgaactttct catcctcaga gtgatggggt 60
tgatagtaca agtcatacag atgtgaaatc tgaccctctg ggtcacccaa attcagagga 120
<210> 377
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 377
aaccgtgaaa gccaaaatac cttctaggca gcaagaagag ctgccaattt attcttctga 60
ttttgaagat gtcccaaata agtcttggca acagaccact ttccaaaaca ggccagatag 120
<210> 378
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 378
tagactggga aaaacagaat tgagtttttc ttcctcttgt gagataccac atgtggatgg 60
cttgcactca tcagaagagc tcagaaactt aggttgggac ttctctcaag aaaagccttc 120
<210> 379
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 379
taccacgtat cagcaacctg acagtagcta tggagcttgt ggtggacaca agtatcagca 60
aaatgcagaa cagtatggtg ggacacgtga ttactggcaa ggcaatggtt actgggatcc 120
<210> 380
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 380
aagatcaggt agacctcctg gaactggggt tgtgtatgat cgaactcaag gacaagtacc 60
agattcccta acagatgatc gtgaagaaga ggagaattgg gatcaacagg atggatccca 120
<210> 381
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 381
tttttcagac cagtccgata aatttcttct atcccttcag aaagacaagg ggtcagtgca 60
agcacctgaa ataagcagca attccattaa ggacacttta gctgtgaatg aaaagaaaga 120
<210> 382
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 382
gaagaaatcc tcacaatctg aaggcatctt tcttggttca gaatctgatg aagattctgt 60
acggacttct tcaagtcaaa gatcacatga tttaaaattt tcagcaagca ttgaaaagga 120
<210> 383
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 383
tttttcaaaa aacttagaaa aaaatgatat caaagataga gggcctctta aaaaaaggag 60
gcaggaaata gagagtgatt ctgaaagtga tggtgagctt caggacagaa agaaagttag 120
<210> 384
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 384
agtggaggta gagcagggag agacatcagt gcccccaggt tcagcactgg ttgggccctc 60
ctgtgtcatg gatgacttca gggacccaca gcgatggaag gaatgtgcca agcaagggaa 120
<210> 385
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 385
aatgccatgt tactttgatc ttattgaaga aaatgtttat ttaacagaaa ggtaagtcta 60
attaatactt gtttgacaaa ttttaactct ttttttgttg ttagaaaaag cctttaaaca 120
<210> 386
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 386
aagagatttt aaaaagagct cagcaccttt aaaaagtgag gatctaggga aaccttcacg 60
atctaaaaca gacagagatg ataaatattt tagctattca aaacttgaaa gagatactcg 120
<210> 387
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 387
gtatgtatct tcccgatgta gatcagaaag agagcgacgg cggagcagat ctcactctag 60
gtctgagaga ggctctagaa ctaatttatc ctattccagg tcagaacgat ctcattatta 120
<210> 388
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 388
tgactctgat cgtcgctacc ataggagctc cccttatcga gagaggacgc gctattctcg 60
gccatacaca gataacagag cacgagagag ttctgactca gaagaagagt ataagaagac 120
<210> 389
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 389
atactcaagg cgtacctcat ctcattcctc ttcttacaga gacctaagga catcatccta 60
ttctaaatct gatcgggact gtaaaactga gacctcttac ttagagatgg aaagaagagg 120
<210> 390
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 390
caagtattct tcaaaactag aaagagaatc taaaaggact tcagaaaatg aagcaattaa 60
aagatgttgt tctcccccta atgaactggg attccgacga gggtcatcat attctaagca 120
<210> 391
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 391
tgacagtagt gcttcccgtt ataaatctac cctttcaaaa cctataccca agtctgataa 60
atttaaaaat tctttctgtt gtacagaatt aaatgaagaa atcaaacagt ctcattcttt 120
<210> 392
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 392
atttgaaagt aactatttat tgtagtagta attgcttatt ttgttcattt gtaggcaaag 60
attgaaaatg tgcagaaaac aggtttcatc aaaggaccaa tgttcaaagg tgttgcttct 120
<210> 393
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 393
agtcgatttt tgcccaaagg caccaaaaca aaagttaatt tggaagaaca gggacgacag 60
aaggtgtcat tcagcttcag ccttacaaag aaaactttgc agaataggtt tctcactgca 120
<210> 394
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 394
cttggcaatg aaaagcaaag tgatactcca aaccctccag ctgtacctct tcaggtagac 60
tcgactccta aaatgaaaat ggaaattggt gataccttat ctactgcaga agaatcttcc 120
<210> 395
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 395
ccaccaaagt caagggtgga attgggcaaa attcatttta agaaacatct gcttcatgta 60
acatccaggc cactgctggc tactaccaca gcagtagcat ctccacctac tcatgcagca 120
<210> 396
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 396
ccattaccag cagtgatagc agaatcaaca actgtagact caccgccctc atctccgcct 60
ccaccgcctc cacctgccca agccacaaca ctctcatcac cagcaccagt aacagagcc 119
<210> 397
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 397
atatctttac caggaaattt atttaatcag tttttatgaa attttaccca atagaaagaa 60
gaataaatct catcgagata ttaagcgaat gcagtgtgag tgtacacctc tttctaaaga 120
<210> 398
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 398
tgaaagagct caaggtgaaa tagcatgtgg ggaagattgt cttaatcgtc ttctcatgat 60
tgaatggtaa gtaaattaga atactcggct tttgctcaac acttctatta aatgtctcta 120
<210> 399
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 399
gttactttgg ttcttttgct gctttaaaga attaaatttc ctccttttct catttcagtt 60
cttctcggtg tccaaatggg gattattgtt ccaatagacg gtttcagaga aaacagcatg 120
<210> 400
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 400
cagatgtgga agtcatactc acagaaaaga aaggctgggg cttgagagct gccaaagacc 60
ttccttcgta agttcttttt taattacctt tcacttcctt ttttgagaca aggtctcac 119
<210> 401
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 401
tatgttttcc ctactgctcc taggtaggaa gtgctatttt tttccttttg gtttgcagga 60
acacctttgt cctagaatat tgtggagagg tactcgatca taaagagttt aaagctcgag 120
<210> 402
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 402
tgaaggagta tgcacgaaac aaaaacatcc attactattt catggccctg aagaatgatg 60
aggtaagcag cttggatatg tttgccttga aacagatctg ttaggtttga aggagccatt 120
<210> 403
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 403
taaatgatcc tcttctccca gataatagat gccactcaaa aaggaaattg ctctcgtttc 60
atgaatcaca gctgtgaacc aaattgtgaa acccaaaaag taagttgagg tggatttaga 120
<210> 404
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 404
catcatctca gtggactgtg aacggacaac tgagggttgg gttttttacc accaaactgg 60
ttccttcagg ctcagagtta acgtttgact atcagttcca gagatatggg taagtattgt 120
<210> 405
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 405
agttttgtat ggtttacttc cacatagctg acttctttcc tatgctcttt gaacagaaaa 60
gaagcccaga aatgtttctg cggatcagcc aattgccggg gttacctggg aggagaaaac 120
<210> 406
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 406
agagtcagca tcagagcagc aggagggaaa atgaagaagg aacgatctcg taagaaggat 60
tcagtaagtt tgcatttttc tttcaacttt cacaataaaa aatttaaaaa gcgaggtggc 120
<210> 407
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 407
tttttttgtt gttgttgttt tataaaaagg tatttttatt tgctgtggta ggtggatgga 60
gagctagaag ctctgatgga aaatggtgag ggtctctctg ataaaaacca ggtgctcagc 120
<210> 408
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 408
ttatcccggc taatggttag aattgaaact ttggagcaga aacttacctg tctggaactc 60
atacaggtga acactttaat gtttgttttt tatttctggt tttgtggtgg gaatgaatga 120
<210> 409
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 409
tgtaatattc tgatattgcc tcccaatgct ctgcatcgtg tctcccttcc cccatgaaag 60
aacacacact cacagtcctg cctgaagtcc tttctggaac gtcatgggct gtctttgttg 120
<210> 410
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 410
tggatctgga tggcagagct aggtgacggc cgggaaagta accagaagct tcaggaagag 60
gtcagttcac atttagcttg cttgtttctt aaaactgtta tattgattgc ctcggttgtg 120
<210> 411
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 411
gatgtaattt ttttttatgt ttaatcagat tataaagact ttggaacact tgcccattcc 60
tactaaaaat atgttggagg aaagcaaagt acttccaatt attcaacgct ggtctcagac 120
<210> 412
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 412
taagactgct gtccctccgt tgagtgaagg agatgggtat tctagtgaga atacatcgcg 60
tgctcataca ccactcaaca cacctgatcc ttccaccaag ctgagcacag aagctgacac 120
<210> 413
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 413
agacactccc aagaaactaa tgtttcgcag actgaaaatt ataagtgaaa atagcatgga 60
cagtgcaatc tctgatgcaa ccagtgagct agaaggcaag gatggcaaag aggatcttga 120
<210> 414
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 414
tcaattagaa aatgtccctg tagaggaaga ggaagaattg cagtcacaac agctactccc 60
acaacagctg cctgaatgca aagttgatag tgaaaccaac atagaagcta gtaagctacc 120
<210> 415
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 415
tacatctgaa ccagaagctg acgctgaaat agagcccaaa gagagcaacg gcacaaaact 60
agaagaacct attaatgaag aaacaccatc ccaagatgaa gaggagggtg tgtctgatgt 120
<210> 416
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 416
ggagagtgaa aggagccaag aacagccaga taaaacagtg gatataagtg atttggccac 60
caaactcctg gacagttgga aagacctaaa ggtaaggaag catcttttgt tcatttcaac 120
<210> 417
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 417
tgatgatccc ccttgtaact tgaaccattt ccatctagct tctcagggca gcaaggagct 60
tgttaacatc accagctgac catgtgtctc cttttctcaa ctccatttcc aacttcgtgc 120
<210> 418
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 418
tgaagaaaaa cgagaaccta gcgtgcattc tgtgaagaaa caggaagatg gttgggaagt 60
tggatgatgc atgaaaaata taataggtga aaagaggaca gaggtggtta actcactggc 120
<210> 419
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 419
gccggcgtta ttttggtgcg tccattgccg acaatggatg tcgttccggg aaaatcccta 60
ctcctggcga gaaaggatta agtgagctac tttttctata cctatataca caggaccact 120
<210> 420
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 420
tcactaataa ccctccctct ggtttatttt ttgcaggagg tatatcgaat tccaaagaaa 60
agtcaaactg aaaaggaaaa cacaagtagg tattcagaga tactctgtgt tgatggcttt 120
<210> 421
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 421
aaacaaacaa accacccttt tcccttagca actgaacgag gaagggatgc tgttggcttc 60
agagatcaaa cacctgcccc gaagactcct aataggtcaa gagagagaga cccagacaag 120
<210> 422
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 422
caaactcaaa ataaagagaa aaggaaacga agaagctccc tctcaccacc ctcttctgcc 60
tatgagcggg gaacaaaaag gccagatgac aggtaagtgg cctgtgtgag tttttttgtt 120
<210> 423
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 423
tttgtggtcc ttattttctt tctagatatg atacaccaac ttctaaaaag aaagtacgaa 60
ttaaagaccg caataaactt tctacagagg aacgccggaa gttgtttgag caagaggtgg 120
<210> 424
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 424
ctcaacggga ggctcagaaa caacagcaac agatgcagaa cctgggaatg acatcaccac 60
tgccctatga ctctcttggt tataatgccc cgcatcatcc ctttgctggt tacccaccag 120
<210> 425
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 425
gttatcccat gcaggcctat gtggatccca gcaaccctaa tgctggaaag gtgctcctgc 60
ccacacccag catggaccca gtgtgttctc ctgctcctta tgatcatgct cagcccttgg 120
<210> 426
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 426
tgggacattc tacagaaccc ctttctgccc ctccaccagt accagtggtg ccacatgtgg 60
cagctcctgt ggaagtttcc agttcccagt atgtggccca gagtgatggt gtagtacacc 120
<210> 427
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 427
aagactccag cgttgctgtc ttgccagtgc cggcccccgg cccagttcag ggacagaatt 60
atagtgtttg ggattcaaac caacagtctg tcagtgtaca gcagcagtac tctcctgcac 120
<210> 428
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 428
agtctcaagc aaccatatat tatcaaggac agacatgtcc aacagtctat ggtgtgacat 60
caccttattc acagacaact ccaccaattg tacaggtaac taattctgaa acttttcttt 120
<210> 429
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 429
ttattaaagt gacaaaagct taaattattg atttcttcat tttatctttt agagttatgc 60
ccagccaagt cttcagtata tccaggggca acagattttc acagctcatc cacaaggagt 120
<210> 430
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 430
ggtggtacag ccagccgcag cagtgactac aatagttgca ccagggcagc ctcagccctt 60
gcagccagta agaaatgttt cagttgttaa aactcattgg ctgtctactt tgtctttgga 120
<210> 431
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 431
cagattaaga gagagatcac tgatcaaaaa tgtgtgtgtt ttctttgtag tctgaaatgg 60
ttgtgacaaa taatctcttg gatctgccgc ccccctctcc tcccaaacca aaaaccattg 120
<210> 432
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 432
tcttacctcc caactggaag acagctcgag atccagaagg gaagatttat tactaccatg 60
tgatcacaag gtaagaggag ctgtatgggc gttcccatgt ctgcttttac ttaatcaaag 120
<210> 433
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 433
ctaggcagac tcagtgggat cctcctactt gggaaagccc aggagatgat gccagccttg 60
agcatgaagc tgagatggac ctgggaactc caacatatga tgaaaacccc atgaaggtga 120
<210> 434
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 434
gttttctcct ccatttttag gcctcgaaaa agcccaagac agcagaagca gacacctcca 60
gtgaactagc aaagaaaagc aaagaagtat tcagaaaaga ggtaaggttt ggtggatata 120
<210> 435
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 435
tgcctgcaga tgtcccagtt catcgtccag tgcctgaacc cttaccggaa acctgactgc 60
aaagtgggaa gaattaccac aactgaagac tttaaacatc tggctcgcaa ggtactcccc 120
<210> 436
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 436
gattggtggc tgtggtctct cttcctgtgc cctggacagc tgactcacgg tgttatgaat 60
aaggagctga agtactgtaa gaatcctgag gacctggagt gcaatgagaa tgtgaaacac 120
<210> 437
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 437
aaaaccaagg agtacattaa gaagtacatg cagaagtttg gggctgttta caaacccaaa 60
gaggacactg aattagagtg actgttgggc cagggtggga ggatgggtgg tcaggtaaga 120
<210> 438
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 438
gctttcacga caagcgcgac gctgaggacg ctatggatgc catggacggg gccgtgctgg 60
acggccgcga gctgcgggtg caaatggcgc gctacggccg ccccccggac tcacaccaca 120
<210> 439
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 439
atttacccag caatactact tttaaaacaa tttttctcct gtagatcttt gccgccgttc 60
attatcagaa gacactgtaa gtattccatc attgttaaac ctaaatttta cacttctgac 120
<210> 440
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 440
tgttaatcca agctgcccag ctctgccgtg aaaagcagca cacaaaagca atagagctgc 60
ttcaggtaaa ataattactt gaatgaggtg tcagacattt gcagctttgt ttcattttca 120
<210> 441
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 441
ggctggcggc tggtgcagcg cggggacccg gcggctttcc gcgcgctggt ggcccagtgc 60
ctggtgtgcg tgccctggga cgcacggccg ccccccgccg ccccctcctt ccgccaggtg 120
<210> 442
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 442
caccccagca gccggtgtct gtgcccggga gaagccccag ggctctgtgg cggcccccga 60
ggaggaggac acagaccccc gtcgcctggt gcagctgctc cgccagcaca gcagcccctg 120
<210> 443
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 443
gcatgccaag ctctcgctgc aggagctgac gtggaagatg agcgtgcggg actgcgcttg 60
gctgcgcagg agcccaggtg aggaggtggt ggccgtcgag ggcccaggcc ccagagctga 120
<210> 444
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 444
cgctgccact caggcccggc ccccgccaca cgctagtgga ccccgaaggc gtctgggatg 60
cgaacgggcc tggaaccata gcgtcaggga ggccggggtc cccctgggcc tgccagcccc 120
<210> 445
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 445
gggcaggacg cgtggaccga gtgaccgtgg tttctgtgtg gtgtcacctg ccagacccgc 60
cgaagaagcc acctctttgg agggtgcgct ctctggcacg cgccactccc acccatccgt 120
<210> 446
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 446
gccccgcctg ccccagcgct actggcaaat gcggcccctg tttctggagc tgcttgggaa 60
ccacgcgcag tgcccctacg gggtgctcct caagacgcac tgcccgctgc gagctgcggt 120
<210> 447
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 447
ctgggcctgg acgatatcca cagggcctgg cgcaccttcg tgctgcgtgt gcgggcccag 60
gacccgccgc ctgagctgta ctttgtcaag gtgggtgccg gggacccccg tgagcagccc 120
<210> 448
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 448
ctctgccggc aggtctctac cttgacagac ctccagccgt acatgcgaca gttcgtggct 60
cacctgcagg agaccagccc gctgagggat gccgtcgtca tcgagcaggt ctgggcactg 120
<210> 449
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 449
ggaggggagc tgggctgggc ctgtgactcc tcagcctctg ttttccccca gggatgtcgc 60
tgggggccaa gggcgccgcc ggccctctgc cctccgaggc cgtgcagtgg ctgtgccacc 120
<210> 450
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 450
aagcattcct gctcaagctg actcgacacc gtgtcaccta cgtgccactc ctggggtcac 60
tcaggacagg caagtgtggg tggaggccag tgcgggcccc acctgcccag gggtcatcct 120
<210> 451
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 451
atttctgaca ttgaggtgtt ccagaagatg ataaagaaat gatagcagct ccagaaatac 60
caactgattt taatctacta cagtaagtaa attatattct gataattttt aaatacttgt 120
<210> 452
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 452
ttcttatctt tctgtcacag ggagtcagaa acacattttt cttctgacac agattttgaa 60
gatatcgaag gaaaaaacca aaagcaaggc aaaggcaaag tatgtatcaa atatttgact 120
<210> 453
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 453
tagtgatata acttaaccac ctcgtatttt ttatgcagac ttgtaaaaaa ggcaaaaagg 60
gcccagcaga aaagggcaaa ggtggaaatg gaggaggaaa acctccttct ggtccaaacc 120
<210> 454
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 454
gaatgaatgg tcatcaccaa cagaatggag tggaaaacat gatgttgttt gaagttgtta 60
aaatgggcaa gagtgctatg caggtaagat ttatgttgtt cttcccagtt catttgtaca 120
<210> 455
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 455
cctctgctgt agtcggtggt agatgattgg atagaatcat acaagcatga ccgagatata 60
gcacttcttg accttatcaa cttttttatt cagtgttcag gctgtaaagg taagatatat 120
<210> 456
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 456
ttctgacatt tgcaaatttc aggagttgtc acagcagaaa tgtttagaca tatgcagaac 60
tctgagataa ttcgaaaaat gactgaagaa ttcgatgagg taacttacta ccttaagtgt 120
<210> 457
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 457
tttaaatttt tgttttagga tagtggagat tatccactta ccatggctgg tcctcagtgg 60
aagaagttca aatccagttt ttgtgaattc attggcgtgt tagtacggca atgtcaatat 120
<210> 458
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 458
agtatcatat atgatgagta tatgatggat acagtcattt cacttcttac aggattgtct 60
gactcacaag tcagagcatt tcgacataca agcaccctgg caggtcggta tttagaaata 120
<210> 459
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 459
gtctgttaga ttagtttcac catactttta ctctttaaaa atagctatga agttgatgac 60
agctttggtg aatgtggcac taaatcttag cattaatatg gataatacac aaagacaata 120
<210> 460
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 460
tgaagcagaa cggaataaaa tgattggaaa acgagccaat gagaggctag aactcctgct 60
acaaaagcgg aaagaggtaa acttttatat tgaatattta ggctattgtg tgaccaactt 120
<210> 461
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 461
agaaatgatg tgttttttta cagcttcagg aaaatcaaga tgaaatagaa aatatgatga 60
atgcaatatt taaaggagtg tttgtacata gataccggta agttgtgaca gtttttttca 120
<210> 462
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 462
cataagtttt gtggtatatt ggcactaatt atgcatcgtt tttccttccc ccattcagtg 60
atgcgatagc tgaaattcga gctatttgca ttgaagagat tggcatttgg atgaagatgt 120
<210> 463
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 463
atagtgatgc ctttcttaat gacagttatt taaaatatgt tggttggact atgcatgata 60
aggtaagatg tgcccttcag actgcttctt tctacacatc ggcgtggctg tctgcacctc 120
<210> 464
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 464
ttttttttag caaggtgaag taagactcaa atgtcttact gctctacaag ggctttatta 60
taacaaagag cttaattcca aactggaact ttttaccagt cggttcaagg ttagtattac 120
<210> 465
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 465
tttaattttt ttgtccttag gatagaattg tgtctatgac ccttgacaaa gaatatgatg 60
ttgcagtaca agcaataaaa ttactcactc ttgttttaca gtaagtatgt atttgttgca 120
<210> 466
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 466
tcttaggagt agtgaagaag ttctcactgc agaagattgt gaaaatgtct atcatctggt 60
ttattcagct caccggccag tagcagtagc agctggagaa tttctctaca aaaagtaaat 120
<210> 467
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 467
acaggctctt cagtcgtaga gatccagagg aggatggaat gatgaaaaga agaggaagac 60
aaggtccaaa tgccaacctt gttaagacat tggttttttt ctttctagaa agtgaggtta 120
<210> 468
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 468
gttacatgag catgcagcat accttgtgga tagcatgtgg gactgtgcta ctgagctgct 60
gaaagactgg gaatgtatga atagcttgtt actggaagag ccacttagtg gagaggaagg 120
<210> 469
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 469
ttttttagca ctaacagata ggcaagagag tgctctgatt gaaataatgc tttgtaccat 60
tagacaagcg gctgaatgtc atcctcccgt gggaagaggg acaggaaaaa gggtatgcca 120
<210> 470
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 470
ttctgttcct ttaggtgctt acagcaaagg agaagaagac acagttggat gataggacaa 60
aaatcactga gctttttgcc gtggcccttc ctcagttatt agcaaaagta agtttgtgtc 120
<210> 471
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 471
ctttatattt cacagtactc tgtagatgca gaaaaggtga ctaacttgtt gcagttgcct 60
cagtactttg atttggaaat atataccact ggacgattag aaaaggtaag attattttgt 120
<210> 472
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 472
aatattatta ttttgcagca tttggatgcc ttattgcgac agatccggaa tattgtagag 60
aagcacacag atacagatgt tttggaagca tgttctaaaa cttaccatgc actctgtaat 120
<210> 473
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 473
gaagagttca caatcttcaa cagagtagat atttcaagaa gtcaactgat agatgaattg 60
gcagataaat ttaaccggct tcttgaagat tttctgcaag aggtatatat tataactatt 120
<210> 474
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 474
ttaatgtata attaatattt atagggtgaa gaacctgatg aagatgatgc atatcaggta 60
ttgtcaacat tgaagaggat cactgctttt cataagtaag ttgaattttg aagttcctgt 120
<210> 475
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 475
tttttttttt ttacagtgcc catgaccttt caaagtggga tttatttgct tgtaattaca 60
aactcttgaa aactggaatc gaaaatggag acatgcctga gcaggttttt atttatttgc 120
<210> 476
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 476
tcctttgatt ctttttacag attgttattc acgcactgca gtgtactcac tatgtaatcc 60
tttggcaact tgctaagata actgaaagca gctctacaaa ggtttgtggt ggttcagtag 120
<210> 477
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 477
taaatttttt caaggaggac ttgctgcgtt taaagaaaca aatgagagta ttttgtcaga 60
tatgtcaaca ttacctgacc aacgtgaata ctactgttaa ggaacaggtt agtaattact 120
<210> 478
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 478
catgctttcc tctttttttt ttttttaaat aggccttcac tattctgtgt gatattttga 60
tgatcttcag ccatcagatt atgtcaggag ggcgtgacat gttagagcca ttagtgtata 120
<210> 479
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 479
cccctgattc ttcattgcag tctgagttgc tcagctttat tttggatcat gtcttcattg 60
aacaggatga tgataataat agtgcaggta attttattgc catcttttta ttaaatctgt 120
<210> 480
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 480
atggttgaaa taaaatacaa gatgcttaat gtttgggact ttttctccag atggtcagca 60
agaggatgaa gccagtaaaa ttgaagctct gcacaagaga agaaatttac ttgcagcatt 120
<210> 481
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 481
ttgtaagcta attgtatata ctgtggtgga gatgaataca gctgcagata tcttcaaaca 60
gtatatgaag gtaaagttga aaaatggata gcaagatagt tttaaaatgc aacgctagtt 120
<210> 482
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 482
tctttatagt attataatga ctatggagat atcatcaaag aaacaatgag taaaacaagg 60
cagatagaca aaattcagtg tgctaagacc cttattctca gtctgcaaca ggtaagcatt 120
<210> 483
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 483
ctccaggtat taagaacttt aacatgcttt ctttctttcc aaacagcttt ttaatgaaat 60
gatacaagaa aatggctata attttgatag atcatcctct acatttagtg gcataaaaga 120
<210> 484
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 484
acttgctcga cgttttgctt taacttttgg acttgatcag ttgaaaacaa gagaagccat 60
tgccatgcta cacaagtaat ctccagatat tttattcagc tctcatattt tttagtcatg 120
<210> 485
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 485
tttttattat agtccctcaa atatttattt caattatttt ttgttttcct ttgcagagat 60
ggcatagaat ttgcttttaa agagcctaat ccgcaagggg agagccatcc acctttaaat 120
<210> 486
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 486
ttggcatttc ttgatattct gagtgaattt tcttctaaac tacttcgaca agacaaaaga 60
acagtgtatg tatttgctgg aaatgtccta tttaatttca ttttgggatt cttaagggca 120
<210> 487
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 487
ttttcaaggt atgtttactt ggaaaagttc atgacctttc agatgtcact ccgaagagag 60
gatgtgtggc ttccactgat gtcttaccga aattctttgc tagctggtgg tgatgatgac 120
<210> 488
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 488
accatgtcag tcattagtgg aatcagcagc cgggggtcaa cagtacggag taaaaaatca 60
aaaccatcta caggaaaacg gaaagtggtt gagggcatgc agctttcact cagtaaggat 120
<210> 489
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 489
cctcgtcgtt aattttcttt tccagctgaa gaaagtagta gtagtgacag tatgtggtta 60
agcagagaac aaacactgca cacccctgtt atgatgcaga caccacaact cacctccact 120
<210> 490
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 490
attatgagag agcccaaaag attacggcct gaggatagct tcatgagtgt ttatccaatg 60
cagactgaac atcatcaaac acctcttgat tataagtaag tacatttgat cattttctgt 120
<210> 491
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 491
gaagcacgca ggtaacatgg atgttagctc aaagacaaca agaggaagca aggcaacagc 60
aggagagagc agcaatgagc tatgttaaac tgcgaactaa tcttcagcat gccatgtaag 120
<210> 492
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 492
gatattaata gtcacgacat tagttcagat cttgactttg ttttatattt ctctagtcgg 60
cgtggcacaa gcctaatgga agatgatgaa gagccaattg tggaagatgt tatgatgtcc 120
<210> 493
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 493
tcagaaggga ggattgagga tcttaatgag ggaatggatt ttgacaccat ggatatagat 60
ttggtaagaa acttaatgat ttctgtaaga ttttcagttt agatcttttg aaaattatgt 120
<210> 494
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 494
ttatttcctt ttttccttta gccaccatca aagaacagac gagagagaac agaactgaag 60
cctgatttct ttgatccagc ttcaattatg gatgaatcag taggtttaat ttaaatgtac 120
<210> 495
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 495
aatgaatgac ttttaacgag attttctccc ctctctctct ctcattaggt tcttggagtg 60
tcaatgtttt aataccagta cacaattaaa tctgtggtga agtcattttc taagtggaag 120
<210> 496
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 496
ataatgctat ccatagcaat gaatttggtc ttttgatttt tcaggagaac ttgcgcctgt 60
caggggctgg atccagaaac ctgtggtgcc tccttctctt ttggttgttc atggagcatg 120
<210> 497
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 497
tccgggtcac tgccatggag gagccgcagt cagatcctag cgtcgagccc cctctgagtc 60
aggaaacatt ttcagaccta tggaaactgt gagtggatcc attggaaggg caggcccacc 120
<210> 498
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 498
acccccaccc caaccccagc cccctagcag agacctgtgg gaagcgaaaa ttccatggga 60
ctgactttct gctcttgtct ttcagacttc ctgaaaacaa cgttctggta aggacaaggg 120
<210> 499
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 499
aggacctggt cctctgactg ctcttttcac ccatctacag tcccccttgc cgtcccaagc 60
aatggatgat ttgatgctgt ccccggacga tattgaacaa tggttcactg aagacccagg 120
<210> 500
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 500
tccagatgaa gctcccagaa tgccagaggc tgctcccccc gtggcccctg caccagcagc 60
tcctacaccg gcggcccctg caccagcccc ctcctggccc ctgtcatctt ctgtcccttc 120
<210> 501
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 501
ccagaaaacc taccagggca gctacggttt ccgtctgggc ttcttgcatt ctgggacagc 60
caagtctgtg acttgcacgg tcagttgccc tgaggggctg gcttccatga gacttcaatg 120
<210> 502
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 502
aaaaaaaaaa aaaaaagaaa agctcctgag gtgtagacgc caactctctc tagctcgcta 60
gtgggttgca ggaggtgctt acgcatgttt gtttctttgc tgccgtcttc cagttgcttt 120
<210> 503
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 503
atctgttcac ttgtgccctg actttcaact ctgtctcctt cctcttccta cagtactccc 60
ctgccctcaa caagatgttt tgccaactgg ccaagacctg ccctgtgcag ctgtgggttg 120
<210> 504
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 504
attccacacc cccgcccggc acccgcgtcc gcgccatggc catctacaag cagtcacagc 60
acatgacgga ggttgtgagg cgctgccccc accatgagcg ctgctcagat agcgatggtg 120
<210> 505
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 505
ttaggtctgg cccctcctca gcatcttatc cgagtggaag gaaatttgcg tgtggagtat 60
ttggatgaca gaaacacttt tcgacatagt gtggtggtgc cctatgagcc gcctgaggtc 120
<210> 506
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 506
cctaggttgg ctctgactgt accaccatcc actacaacta catgtgtaac agttcctgca 60
tgggcggcat gaaccggagg cccatcctca ccatcatcac actggaagac tccaggtcag 120
<210> 507
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 507
taggacctga tttccttact gcctcttgct tctcttttcc tatcctgagt agtggtaatc 60
tactgggacg gaacagcttt gaggtgcgtg tttgtgcctg tcctgggaga gaccggcgca 120
<210> 508
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 508
cagaggaaga gaatctccgc aagaaagggg agcctcacca cgagctgccc ccagggagca 60
ctaagcgagg taagcaagca ggacaagaag cggtggagga gaccaagggt gcagttatgc 120
<210> 509
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 509
acctttcctt gcctctttcc tagcactgcc caacaacacc agctcctctc cccagccaaa 60
gaagaaacca ctggatggag aatatttcac ccttcaggta ctaagtcttg ggacctctta 120
<210> 510
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 510
aacttatacc ataatatata ttttaaagga ccagaccagc tttcaaaaag aaaattgtta 60
aagagagcat gaaaatggtt ctatgacttt gcctgataca gatgctactt gacttacga 119
<210> 511
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 511
ctgcagatcc gtgggcgtga gcgcttcgag atgttccgag agctgaatga ggccttggaa 60
ctcaaggatg cccaggctgg gaaggagcca ggggggagca gggctcactc caggtgagtg 120
<210> 512
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 512
ctgcttctgt ctcctacagc cacctgaagt ccaaaaaggg tcagtctacc tcccgccata 60
aaaaactcat gttcaagaca gaagggcctg actcagactg acattctcca cttcttgttc 120
<210> 513
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 513
tcattttccc ttacagagtc aactgttcat tttatttcaa aattggagca tgtcgtcatg 60
gagacaggtg ctctcggttg cacaataaac cgacgtttag ccaggtttgt ttgccttttt 120
<210> 514
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 514
cgatccacgc cgagctgtca cccgtgacgg acttcagaga agcctgctgc cgtcagtatg 60
agatggggtg agtgaggagt ggccagtgac gtgactgagc acagttcggg gccgtgtgca 120
<210> 515
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 515
ctctgtccat ttaggtgtga aaccattcca gtgtaaaact tgtcagcgaa agttctcccg 60
gtccgaccac ctgaagaccc acaccaggac tcatacaggt aaaacaagtg cgtaaacttt 120
<210> 516
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 516
ggcgtcatgg actactacct gaagtacttc agcggcgccc acgacggcga cgtctacccg 60
gcctggtcgc agaaggtgga gaagaagatc cgagccaagg ccttccagaa gtgccccatc 120
<210> 517
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 517
tgcgagaagg tcatccaggg cgccggcaag ctgccgcgac acatccgcac ccacacgggc 60
gagaagccct acgagtgcaa catctgcaag gtccgcttca ccaggtgagc tgcccgccgc 120
<210> 518
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 518
gtagatcaaa ttgatcagcg caacctcctc cgcgccaagg agtacctcga gttcttccag 60
agcaacccca tgaacagcct gccccccgcg gccgccgccg ccgctgccag cttcccgtgg 120
<210> 519
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 519
tttcctgcca gacctggagg ggcggggcgg tgccggcaag atggctgcgc ccgagaagat 60
gacgtttccc gagaaaccaa ggtaagcgcc gtacggggag atgagcaggc gagcgggccg 120
<210> 520
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 520
tatatgtctt aatcttccag ccacaaaaag tacagggccg ccctgaagaa ggagaaacga 60
aagaaacgtc ggcaggaact tgctcgactg agagactcag gtgatggact ctttattctg 120
<210> 521
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 521
aaacatttaa attccctagg actctcacag aaggaggaag aggaggacac ttttattgaa 60
gaacaacaac tagaagaaga gaagctattg gaaagagaga ggtcagtgct aattgaaaca 120
<210> 522
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 522
tgtgtgtgtg tatttgtgga ttaatgccca tttcttttat ctgattacat cattatactg 60
ataggcaaag attacatgag gagtggttgc taagagagca gaaggcacaa gaagaattc 119
<210> 523
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 523
aagaaggaaa aggaagaggc ggctaaaaaa cggcaagaag aacaagaggt atggtaggaa 60
tcacgtaact agtgaacaaa ctgattactt caccataata actctcatga agagttgcct 120
<210> 524
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 524
ttcatctatg tgcgctgtat gtgaaatgtt tttaaaaagt gactgaaaca aagttacttt 60
ttcttcccct ttaaagagaa agttaaagga acaatgggaa gaacagcaga ggaaagaga 119
<210> 525
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 525
caggagaaga aagaaaaaga ggtgattcct gtcatgggat gtgctgtgtg atgagtttga 60
agaataatca gtaggcatgt cagagtttgg gttttttttt tctctttttc ttgtcatttc 120
<210> 526
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 526
cttctgggtt tttattagtc ctgtgtttgc accattttag gaagctttgc agaagatgct 60
ggatcaggct gaaaatgagg tatttttaaa accttacatt gataatttga gaccaattaa 120
<210> 527
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 527
ttggaaaatg gtaccacatg gcaaaaccca gaaccacccg tggatttcag agtaatggag 60
aaggatcgag ctaattgtcc cttctacagt aaaacaggag cttgcagatt tggagatagg 120
<210> 528
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 528
ctttcatacg cagatgttca cgtaaacata atttcccaac atccagtcct acccttctta 60
ttaagagcat gtttacgacg tttggaatgg agcagtgcag gagggatgac tatgaccctg 120
<210> 529
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 529
acgcaagcct ggagtacagc gaggaagaaa cctaccaaca gttcctagac ttctatgagg 60
atgtgttgcc cgagttcaag aacgtgggga aagtgattca gttcaaggtg ggcatgcgtg 120
<210> 530
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 530
tttttatgat agcattcttc attgctccct aggtcagctg caatttggaa cctcacctga 60
ggggcaatgt atatgttcag taccagtcgt aagtattctg cttgtggatg tcttcctgat 120
<210> 531
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 531
taaagggaag aagaatgcca agcagccctt tctctgttta acggacgatg gtatgcagga 60
cgacagctgc agtgtgaatt ctgccccgtg acccggtgga aaatggcgat ttgtggtaaa 120
<210> 532
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 532
gaaaatagtt ctagcatatc caaatatcag aaatgtacct tcggaaaagg ataaagtagc 60
atcttcagaa tatgcagtga taagtgctgt ttcatcactg tgccattgca ggtttatttg 120
<210> 533
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 533
aaatacaaca atgtccaaga ggaaagcact gcaactttct tcatgtgttc agaaatccca 60
acaatgaatt ctgggaagct aatagagaca tctacttgtc tccagatcgg actggctcct 120
<210> 534
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 534
cctttgggaa gaactccgaa aggagggaga ggatgggcca ccacgacgac tactacagca 60
ggctgcgggg aaggagaaac cctagtccag accactccta caaaagaaat ggggaatccg 120
<210> 535
<211> 119
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 535
agaggaaaag tagtcgtcac agggggaaga aatctcacaa acgcacatca aagagtcggg 60
agaggcacaa ttcacgaagc agaggaagaa atagggaccg cagcagggac cgcagccgg 119
<210> 536
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 536
cggagccgga gccggagccg caggagccgc cgcagccgga gccaaagttc ctctaggtcc 60
cgaagtcgtg gcaggaggag gtcgggtaat agagacagaa ctgttcagag tcccaaatcc 120
<210> 537
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 537
aaataaacta gttttgttct taaatgattg tatatcttat ttattatggt tgctacatac 60
tcggatagaa ggctatgaac gcagatgggt tcgcaagtct ggctgaaccc tgtgtagcct 120
<210> 538
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 538
gtttgcttac taatttttga tttcttcatt ttttataggg tctattgccg aaaacacaag 60
aaaactgcac ataactccga aggtacatca tttagccacg tttcagccac ttttcagttt 120
Claims (7)
1.一种急性髓系白血病相关基因群检测的探针组合物,其特征在于:所述的探针组合物包括SEQ ID NO:1-SEQ ID NO:538所示的序列。
2.权利要求1所述的探针组合物在制备急性髓系白血病相关基因群检测产品中的应用。
3.一种急性髓系白血病相关基因群检测的产品,其特征在于:所述的产品包括权利要求1所述的探针组合物。
4.根据权利要求3所述的产品,其特征在于:所述的产品包括独立试剂、芯片或试剂盒。
5.权利要求1所述的探针组合物或权利要求3-4任一项所述的产品在检测急性髓系白血病相关基因群突变中的应用。
6.一种检测急性髓系白血病相关基因群突变的方法,所述的方法为非疾病诊断和治疗方法,其特征在于:所述的方法包括利用权利要求1所述的探针组合物或权利要求3-4任一项所述的产品对待测样本中急性髓系白血病相关基因群的突变进行检测。
7.根据权利要求6所述的方法,其特征在于:所述的方法包括以下步骤:
(1)血液样本单个核细胞分离;
(2)样本DNA提取;
(3)文库构建;
(4)上机测序;
(5)数据分析。
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CN202210162686.9A CN115323051B (zh) | 2022-02-22 | 2022-02-22 | 一种急性髓系白血病检测探针组合物及其应用 |
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CN202210162686.9A CN115323051B (zh) | 2022-02-22 | 2022-02-22 | 一种急性髓系白血病检测探针组合物及其应用 |
Publications (2)
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006119494A2 (en) * | 2005-05-04 | 2006-11-09 | Exagen Diagnostics, Inc. | Acute myelogenous leukemia biomarkers |
CN101851683A (zh) * | 2010-06-30 | 2010-10-06 | 协和干细胞基因工程有限公司 | 一种用于检测急性髓系白血病化疗疗效相关的基因组合、引物、探针和用途 |
WO2013138237A1 (en) * | 2012-03-12 | 2013-09-19 | Memorial Sloan-Kettering Cancer Center | Methods and compositions for the diagnosis, prognosis and treatment of acute myeloid leukemia |
WO2013148450A1 (en) * | 2012-03-30 | 2013-10-03 | The Board Of Trustees Of The Leland Stanford Junior University | Use of jam-a in diagnosing and treating leukemia |
CN107287345A (zh) * | 2017-08-24 | 2017-10-24 | 中国人民解放军总医院 | 用于急性髓系白血病精准诊疗的检测试剂盒及tsen34临床应用 |
CN111154881A (zh) * | 2020-03-09 | 2020-05-15 | 南京实践医学检验有限公司 | 一种急性髓系白血病中基因突变的检测试剂盒及应用 |
-
2022
- 2022-02-22 CN CN202210162686.9A patent/CN115323051B/zh active Active
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006119494A2 (en) * | 2005-05-04 | 2006-11-09 | Exagen Diagnostics, Inc. | Acute myelogenous leukemia biomarkers |
US20060281107A1 (en) * | 2005-05-04 | 2006-12-14 | Exagen Diagnostics, Inc. | Acute myelogenous leukemia biomarkers |
CN101851683A (zh) * | 2010-06-30 | 2010-10-06 | 协和干细胞基因工程有限公司 | 一种用于检测急性髓系白血病化疗疗效相关的基因组合、引物、探针和用途 |
WO2013138237A1 (en) * | 2012-03-12 | 2013-09-19 | Memorial Sloan-Kettering Cancer Center | Methods and compositions for the diagnosis, prognosis and treatment of acute myeloid leukemia |
CN104508143A (zh) * | 2012-03-12 | 2015-04-08 | 纪念斯隆-凯特琳癌症中心 | 用于急性髓细胞白血病的诊断、预后和治疗的方法和组合物 |
WO2013148450A1 (en) * | 2012-03-30 | 2013-10-03 | The Board Of Trustees Of The Leland Stanford Junior University | Use of jam-a in diagnosing and treating leukemia |
CN107287345A (zh) * | 2017-08-24 | 2017-10-24 | 中国人民解放军总医院 | 用于急性髓系白血病精准诊疗的检测试剂盒及tsen34临床应用 |
CN111154881A (zh) * | 2020-03-09 | 2020-05-15 | 南京实践医学检验有限公司 | 一种急性髓系白血病中基因突变的检测试剂盒及应用 |
Non-Patent Citations (2)
Title |
---|
CATERINA MARCONI 等: "5’UTR point substitutions and N-terminal truncating mutations of ANKRD26 in acute myeloid leukemia", 《JOURNAL OF HEMATOLOGY & ONCOLOGY》 * |
周柰岑 等: "伴 ASXL1 基因突变的急性髓系白血病患者的临床特征及预后分析", 《中国实验血液学杂志 》 * |
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