CN115318813A - Method for promoting plants to extract Cd from red soil in mining area - Google Patents
Method for promoting plants to extract Cd from red soil in mining area Download PDFInfo
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- CN115318813A CN115318813A CN202211074889.9A CN202211074889A CN115318813A CN 115318813 A CN115318813 A CN 115318813A CN 202211074889 A CN202211074889 A CN 202211074889A CN 115318813 A CN115318813 A CN 115318813A
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- mining area
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- 239000002689 soil Substances 0.000 title claims abstract description 55
- 238000005065 mining Methods 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 33
- 230000001737 promoting effect Effects 0.000 title claims abstract description 24
- 241000196324 Embryophyta Species 0.000 claims abstract description 28
- 244000104272 Bidens pilosa Species 0.000 claims abstract description 27
- 235000010662 Bidens pilosa Nutrition 0.000 claims abstract description 27
- 239000003623 enhancer Substances 0.000 claims abstract description 21
- 238000000605 extraction Methods 0.000 claims abstract description 18
- 230000012010 growth Effects 0.000 claims abstract description 8
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 6
- 238000003306 harvesting Methods 0.000 claims abstract description 3
- 238000009331 sowing Methods 0.000 claims description 11
- 150000001875 compounds Chemical class 0.000 claims description 8
- 241001474374 Blennius Species 0.000 claims description 7
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 241000195474 Sargassum Species 0.000 claims description 2
- 230000002708 enhancing effect Effects 0.000 claims 1
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 7
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 239000002028 Biomass Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000002344 surface layer Substances 0.000 description 5
- 238000011160 research Methods 0.000 description 4
- 230000036541 health Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 241000143476 Bidens Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 229910020218 Pb—Zn Inorganic materials 0.000 description 1
- 241000196252 Ulva Species 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 231100000003 human carcinogen Toxicity 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a method for promoting plants to extract Cd from red soil in a mining area by using an enhancer, which comprises the following steps: the method comprises the steps of planting bidens pilosa in Cd-polluted red soil of a mining area, preparing, uniformly injecting a reinforcer into the soil in a vigorous growth period, harvesting and safely treating the bidens pilosa in a fruit period. The invention utilizes the alga extract-CaCl 2 The method for promoting the bidens pilosa to extract the red soil Cd in the mining area not only promotes the growth of the bidens pilosa, but also promotes the absorption and enrichment capacity of the bidens pilosa to the red soil Cd in the mining area, particularly improves the extraction efficiency of the bidens pilosa to the red soil Cd in the mining area, and provides new reference, new thought, new method and new technology for the phytoremediation of the heavy metal contaminated soil.
Description
Technical Field
The invention relates to the field of restoration of heavy metal contaminated soil in a mining area, and particularly relates to a method for promoting plants to extract Cd from red soil in the mining area.
Background
Heavy metals in 'three wastes' in mining industry have serious influence and harm on the environment. They migrate, transform and concentrate in the soil around the mine site through multiple pathways, under the dual influence of natural action and human activities. This makes the soil surrounding the mine an important "sink" for heavy metals. Meanwhile, the heavy metals in the soil around the mining area enter a biological chain under the action of epibiotic geochemistry, biology and the like, and influence the ecological system and human health through the biological amplification effect. Among these heavy metals, cd is one of the food contaminants that is being highlighted by the world health organization. Also, international agency for research on cancer (IARC) has classified it as a human carcinogen; the U.S. poison and disease registration Agency (ATSDR) ranks it as the 7 th substance that is hazardous to human health. Therefore, the method has extremely important practical significance in restoring Cd-polluted soil in the mining area.
Plant extraction is an important technology for restoring Cd-polluted soil in a mining area, and the restoration purpose is realized mainly by reducing the content of Cd in the soil by utilizing the absorption, transportation and enrichment functions of plants. The method can thoroughly remove Cd from soil, is convenient to operate, has relatively low price and has great development potential. However, the Cd absorption capacity of plants is limited, the removal efficiency is low, and the plants are difficult to be applied to actual soil improvement. Researches find that certain promoters can not only promote and restore plant growth, but also enhance the capacity and efficiency of plants for extracting Cd from soil, however, research and development and selection of cheap, efficient, green and environment-friendly promoters are still technical bottlenecks. Meanwhile, the method for promoting the extraction of the specific type of soil Cd by the specific plant is still a key technical problem worthy of research and urgent solution.
Disclosure of Invention
The invention aims to overcome the technical defects and provides a method for promoting plants to extract Cd from red soil in a mining area, and the technical scheme provided by the invention comprises the following steps:
a, sowing and planting bidens pilosa in Cd-polluted red soil of a mining area;
b, preparing a reinforcer for promoting the plants to extract the Cd in the red soil of the mining area;
c, applying a reinforcer for promoting plant extraction to the Cd-polluted red soil of the mining area;
d harvesting and safely disposing the bidens pilosa in the fruit stage.
Preferably, the row spacing of the sown and planted bidens pilosa in the step a of the method is 20-30cm, and the sowing quantity is 0.6-0.9 kg/mu.
Preferably, the enhancer for promoting the plants to extract the red soil Cd in the mining area in the step b of the method is 0.1-0.2mol/L of seaweed extract-CaCl 2 A compound is provided.
Preferably, the mode of applying the enhancer for promoting plant extraction in the step c of the method is uniform injection application, and the injection application is performed at the time of 5-6L/m after the vigorous growth period 2 。
Preferably, the applying of the enhancer for promoting plant extraction in step c of the method is injection application to the soil with 0-20cm of surface layer at a grid density of 5 cm.
The method for promoting the plants to extract the red soil Cd in the mining area has the following beneficial effects:
1. the method can promote the absorbing and enriching capacity of bidens pilosa to Cd in the red soil of the mining area;
2. the method can promote the growth of the bidens pilosa;
3. the method can promote the extraction capacity of bidens pilosa to Cd in red soil in a mining area;
4. the method disclosed by the invention is simple and convenient to operate, low in cost and environment-friendly, and can be used for in-situ continuous repair.
Drawings
FIG. 1 is a flow diagram of a method of the present invention for promoting plant extraction of red soil Cd in a mining area;
FIG. 2 shows that the method for promoting the plants to extract Cd from the red soil of the mining area is applied to the bidens pilosaInfluence of the enrichment factor of Cd in grass, wherein the multiple of the enrichment factor is the application of enhancer (seaweed extract-CaCl) 2 Compound) to the ratio of the enrichment factor after compounding) to the unapplied enrichment factor;
FIG. 3 shows the effect of the method for promoting the plant to extract Cd from red soil in a mining area on the biomass of Bidens pilosa, wherein the biomass multiple is the application of a reinforcer (seaweed extract-CaCl) 2 Formulation) versus biomass after application;
FIG. 4 is a graph showing the effect of the method for promoting the plants to extract the red soil Cd from the mining area on the extraction efficiency of the red soil Cd from the mining area, wherein the extraction efficiency is multiplied by applying a reinforcer (seaweed extract-CaCl) 2 Compound) and the ratio of the extraction rate of Cd in red soil to the extraction rate not applied.
Detailed description of the invention
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.
Applicants have discovered that seaweed extract-CaCl 2 The compound has effect in promoting herba Bidentis Bipinnatae to extract Cd from red soil in mining area, and provides a method for extracting CaCl from seaweed extract 2 The method for promoting bidens pilosa to extract Cd from the red soil in the mining area by the compound can provide new reference, new thinking, new method and new technology for phytoremediation of heavy metal contaminated mining area soil.
The present application will be described in detail below with reference to the embodiments with reference to the attached drawings. The preparation method of the enhancer comprises freezing desalted and air dried green tide algae (mainly Enteromorpha) at-18 deg.C to-25 deg.C for 24 hr, grinding to 0.15-0.125mm, leaching with 80 times of hot water at 95 deg.C, naturally cooling to room temperature, mixing with calcium chloride at 0.1mol/L ratio, magnetically stirring at 60-80 deg.C for 60min, and cooling to room temperature to obtain Sargassum extract-CaCl 2 The compound is the enhancer.
Example 1
Sowing Bidens pilosa at row spacing of 30cm and sowing rate of 0.9 kg/mu, and performing normal field management; when herba Bidentis Bipinnatae enters into vigorous growth stage, the surface layer 0-20cm soil is uniformly distributed according to 5cm grid densityThe concentration of the enhancer is 0.05mol/L, and the application amount of the enhancer is controlled at 6L/m 2 (ii) a Bidens pilosa is harvested in the fruit stage and safely disposed.
Example 2
Sowing Bidens pilosa at row spacing of 30cm and sowing rate of 0.9 kg/mu, and performing normal field management; when the bidens pilosa enters the vigorous growth period, uniformly injecting and applying the enhancer to the soil with 0-20cm surface layer according to the grid density of 5cm, wherein the concentration of the enhancer is 0.1mol/L, and the application amount of the enhancer is controlled to be 6L/m 2 (ii) a Bidens pilosa is harvested in the fruit stage and safely disposed.
Example 3
Sowing Bidens pilosa at row spacing of 30cm and sowing rate of 0.9 kg/mu, and performing normal field management; when the bidens pilosa enters the vigorous growth period, uniformly injecting and applying the enhancer to the soil with 0-20cm surface layer according to the grid density of 5cm, wherein the concentration of the enhancer is 0.2mol/L, and the application amount of the enhancer is controlled to be 6L/m 2 (ii) a Bidens pilosa is harvested in the fruit stage and safely disposed.
Test examples
The applicant selects a certain Pb-Zn mining area Cd-polluted red soil as a research object, and arranges a blank control group, an experimental group 1, an experimental group 2 and an experimental group 3, wherein each group is repeated for 3 times, the blank control group does not apply a reinforcer, and the experimental groups 1, 2 and 3 are treated as in examples 1, 2 and 3.
The content of Cd in each group of Bidens pilosa is tested and analyzed, meanwhile, surface layer (0-20 cm) soil samples are collected and the content of Cd is tested and analyzed, the results of the multiple enrichment coefficient (the ratio of the content of Cd in a plant to the content of Cd in the soil) and the multiple extraction rate (the difference of the content of Cd in the soil before and after applying the enhancer is the percentage of the content of Cd in the soil before applying the enhancer) are shown in the figures 2-4, and the data results are as follows:
| fortifier concentration (mol/L) | 0.05 | 0.1 | 0.2 |
| Multiple of enrichment factor | 1.39 | 3.11 | 2.72 |
| Multiple of biomass | 1.13 | 1.28 | 1.28 |
| Multiple of extraction rate | 1.27 | 2.63 | 2.47 |
The results show that when the enrichment coefficient, the biomass and the extraction efficiency of Cd in red soil in a mining area are compared, the enrichment coefficient multiple, the biomass multiple and the extraction efficiency multiple of the sample can reach the maximum when the concentration of the enhancer is 0.1mol/L, and the effect is obviously better than 0.05mol/L when the concentration of the enhancer is 0.1mol/L and 0.2mol/L, and is obviously higher than that of a blank control group.
The present invention has been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above-described embodiments, and it is possible for those skilled in the art to make other additions and modifications without departing from the gist of the present invention. Furthermore, those skilled in the art will appreciate that the foregoing description is by way of example only, and is not intended to limit the invention.
Claims (7)
1. A method for promoting plants to extract Cd from red soil in a mining area is characterized by comprising the following steps:
a, sowing and planting bidens pilosa in Cd-polluted red soil of a mining area;
b, preparing a reinforcer for promoting the plants to extract the Cd in the red soil of the mining area;
c, applying a reinforcer for promoting plant extraction to the Cd-polluted red soil of the mining area;
d harvesting and safely disposing the bidens pilosa in the fruit stage.
2. The method of claim 1, wherein said sowing of Bidens pilosa in step a is carried out at a row spacing of 20-30cm and a sowing rate of 0.6-0.9 kg/mu.
3. The method as claimed in claim 1, wherein the enhancer for promoting the plant to extract Cd from red soil in the mining area in step b of the method is 0.1-0.2mol/L seaweed extract-CaCl 2 A compound is provided.
4. The method according to claim 1, wherein said applying of the enhancer for enhancing plant extraction in step c is by injection application to a soil surface of 0-20cm at a grid density of 5 cm.
5. The method of claim 4, wherein the injection is administered at a time after the period of vigorous growth in an amount of 5-6L/m 2 。
6. Sargassum extract-CaCl 2 The application of the compound in promoting plants to extract Cd from red soil in a mining area.
7. The use of claim 6, wherein said plant is Bidens pilosa.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211074889.9A CN115318813A (en) | 2022-09-02 | 2022-09-02 | Method for promoting plants to extract Cd from red soil in mining area |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211074889.9A CN115318813A (en) | 2022-09-02 | 2022-09-02 | Method for promoting plants to extract Cd from red soil in mining area |
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| CN202211074889.9A Pending CN115318813A (en) | 2022-09-02 | 2022-09-02 | Method for promoting plants to extract Cd from red soil in mining area |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147914A (en) * | 2006-09-22 | 2008-03-26 | 中国科学院沈阳应用生态研究所 | Method for repairing cadmium pollution soil using with asteraeae plant |
| CN108526216A (en) * | 2018-04-24 | 2018-09-14 | 四川农业大学 | A method of improving bidens pilosa cadmium pollution repair ability |
| CN110055082A (en) * | 2019-05-07 | 2019-07-26 | 浙江绿垚生态环境有限公司 | A kind of heavy-metal contaminated soil repairs medicament and its application method |
| CN110205139A (en) * | 2019-04-29 | 2019-09-06 | 鲁东大学 | A kind of green soil Cd activator and preparation method thereof |
| AU2021105128A4 (en) * | 2021-08-09 | 2021-10-07 | Ludong University | Acid soil activator for cadmium, copper and zin contaminated soil and preparation method thereof |
-
2022
- 2022-09-02 CN CN202211074889.9A patent/CN115318813A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147914A (en) * | 2006-09-22 | 2008-03-26 | 中国科学院沈阳应用生态研究所 | Method for repairing cadmium pollution soil using with asteraeae plant |
| CN108526216A (en) * | 2018-04-24 | 2018-09-14 | 四川农业大学 | A method of improving bidens pilosa cadmium pollution repair ability |
| CN110205139A (en) * | 2019-04-29 | 2019-09-06 | 鲁东大学 | A kind of green soil Cd activator and preparation method thereof |
| CN110055082A (en) * | 2019-05-07 | 2019-07-26 | 浙江绿垚生态环境有限公司 | A kind of heavy-metal contaminated soil repairs medicament and its application method |
| AU2021105128A4 (en) * | 2021-08-09 | 2021-10-07 | Ludong University | Acid soil activator for cadmium, copper and zin contaminated soil and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张云霞;周浪;肖乃川;庞瑞;宋波;: "鬼针草(Bidens pilosa L.)对镉污染农田的修复潜力", 生态学报 * |
| 胡国涛;杨兴;陈小米;陆扣萍;何丽芝;叶正钱;吴晓红;王海龙;: "速生树种竹柳对重金属胁迫的生理响应", 环境科学学报 * |
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