CN115317548A

CN115317548A - Processing method for enhancing effect of barbary wolfberry fruit for treating eye diseases in interior and application of processing method

Info

Publication number: CN115317548A
Application number: CN202210922077.9A
Authority: CN
Inventors: 张霞; 高欣; 施杰; 都青钰; 和敏; 张彩芳; 李治芳
Original assignee: Ningxia Medical University
Current assignee: Ningxia Medical University
Priority date: 2022-08-02
Filing date: 2022-08-02
Publication date: 2022-11-11
Anticipated expiration: 2042-08-02
Also published as: CN115317548B

Abstract

The invention discloses a processing method for enhancing the effect of barbary wolfberry fruit in treating ophthalmopathy, which comprises the following steps: parching fructus Lycii 4 parts with fructus Zanthoxyli, semen Sesami, herba Menthae and fructus Foeniculi respectively, sieving to remove fire, and mixing. The invention adds yellow wine into the medlar for soaking, and the medlar is respectively fried with pepper, sesame, mint and fennel, thereby being capable of replenishing vital essence and invigorating yang; the volatile components in the processing auxiliary materials are added in the processed medlar, and have the functions of ascending and dispersing, thereby being beneficial to guiding the medicine upwards and further improving the efficacy of the medlar in treating eye diseases in a prescription.

Description

Processing method for enhancing effect of barbary wolfberry fruit for treating eye diseases in interior and application of processing method

Technical Field

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a processing method for enhancing the effect of barbary wolfberry fruit in treating eye diseases and application thereof.

Background

Along with the high intensity pressure brought by work, study and life, the rapid infiltration of high-tech electronic products, the accumulation of factors such as large-scale pollution of living environment and the like, more and more people have discomfort or diseases of eyes, the traditional Chinese medicine accumulates abundant experience in the aspect of treating the diseases of the eyes and has unique curative effect and advantages; in the zang-fu organs of the human body, the liver and kidney are closely related to the eyes, and the deficiency of the liver and kidney can lead to the deficiency of essence and blood which are the sources of eye nourishing, thus causing various cataract eye diseases.

In long-term research practice, researchers of traditional Chinese medicines find that after the traditional Chinese medicines are properly processed, active ingredients can be added, and pharmacological effects can be improved. Medlar is sweet and mild in flavor, can reduce deficiency heat, can supplement essence and marrow, and is used for treating blurred vision and nebula; the medlar is a food material used as both medicine and food, people usually eat the medlar by soaking in water for drinking, and then the medlar is put in soup for cooking or is combined with other medicinal materials for compound use, but the technical effect on treating eye diseases is deviated. Although the prior art adopts stir-frying, salt processing or wine processing and other processing methods, the effect is not satisfactory.

Therefore, the problem to be solved urgently by the technical personnel in the field is to provide a processing method for enhancing the efficacy of barbary wolfberry fruit for treating eye diseases.

Disclosure of Invention

In view of the above, the invention provides a processing method for enhancing the effect of barbary wolfberry fruit on treating eye diseases in the prescription and application thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

a processing method for enhancing the effect of barbary wolfberry fruit in treating eye diseases comprises the following steps: parching fructus Lycii 4 parts with fructus Zanthoxyli, semen Sesami, herba Menthae and fructus Foeniculi respectively, sieving to obtain fructus Lycii, and mixing.

Preferably, the mass ratio of the wolfberry fruit to the pepper, the sesame, the mint and the fennel is 5-10.

Preferably, the Chinese wolfberry is soaked by yellow wine before being fried.

Preferably, the mass ratio of the medlar to the yellow wine is 5-15.

Preferably, the stir-frying is carried out until the rhubarb is fried.

The processed fructus Lycii obtained by the above preparation method.

The application of the wolfberry in preparing the medicines for preventing or treating the eye diseases is disclosed.

Preferably, the medicine further comprises radix rehmanniae recen, prepared rehmannia root, white poria, bighead atractylodes rhizome and chrysanthemum.

Preferably, the mass ratio of the wolfberry fruit, the radix rehmanniae recen, the prepared rehmannia root, the white poria cocos, the white atractylodes rhizome and the chrysanthemum is 5-15.

Preferably, the dosage form of the medicine is any one of pills, granules, tablets, powder and paste.

According to the technical scheme, compared with the prior art, the invention has the following beneficial effects: according to the invention, yellow wine is added into the medlar for soaking, the medlar is respectively fried with pepper, sesame, mint and fennel, the medlar is screened out, fire and smell are removed, and the effect of treating eye diseases in a medlar prescription can be improved; the traditional Chinese medicine considers that: the left kidney, yin water and yin deficiency can cause the yang fire to be treated independently and to generate flower; the wolfberry fruit is soaked in yellow wine and fried with the four aromatic medicinal materials to replenish essence and tonify yang, and the right kidney is matched with the four aromatic medicinal materials; adding Atractylodis rhizoma, poria, flos Chrysanthemi, and rehmanniae radix for promoting diuresis and yin production, and regulating left kidney; patients with the disease also seek to be healed after the two fluids are full of water and the five fire is latent. The compound has effects of nourishing liver and kidney, clearing heat of deficiency type, and improving eyesight, and can be used for treating deficiency of both kidney and eye, and cataract.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and the drawings in the description are only the embodiments of the present invention.

FIG. 1 is a GC-MS spectrum of comparative example 1 of fructus Lycii of the present invention;

FIG. 2 is a GC-MS spectrum of comparative example 2 of fructus Lycii of the present invention;

FIG. 3 is a GC-MS spectrum of comparative example 3 of fructus Lycii of the present invention;

FIG. 4 is a GC-MS analysis spectrum of the black sesame fried wolfberry of the present invention;

FIG. 5 is a GC-MS analysis spectrum of the mint-fried wolfberry of the present invention;

FIG. 6 is a GC-MS analysis spectrum of fructus Lycii parched with pericarpium Zanthoxyli of the present invention;

FIG. 7 is a GC-MS analysis spectrum of fructus Lycii parched with fructus Foeniculi according to the present invention;

FIG. 8 is an ultra high performance liquid chromatography-high resolution mass spectrum of Lycium barbarum of comparative example 1 of the present invention;

FIG. 9 shows the ultra high performance liquid chromatography-high resolution mass spectrum of Lycium barbarum of comparative example 2;

FIG. 10 is the ultra high performance liquid chromatography-high resolution mass spectrogram of fructus Lycii of comparative example 3;

FIG. 11 is an ultra-high performance liquid chromatography-high resolution mass spectrogram of fructus Lycii parched with fructus Zanthoxyli of the present invention;

FIG. 12 is an ultra high performance liquid chromatography-high resolution mass spectrum of the black sesame fried medlar of the present invention;

FIG. 13 is an ultra high performance liquid chromatography-high resolution mass spectrum of fructus Lycii parched with fructus Foeniculi according to the present invention;

FIG. 14 is an ultra high performance liquid chromatography-high resolution mass spectrum of the fried fructus Lycii with peppermint of the present invention;

FIG. 15 is a graph of HE staining of a retina of the present invention;

wherein, A is a control group, B is a model group, C is a raw medlar prescription group, and D is a processed medlar prescription group.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The preparation method of the processing method for enhancing the effect of barbary wolfberry fruit in treating eye diseases specifically comprises the following steps:

preparing four equal parts of wolfberry, adding yellow wine into each part of wolfberry, wherein the mass ratio of the wolfberry to the yellow wine is 12;

then adding radix rehmanniae, radix rehmanniae preparata, poria alba, bighead atractylodes rhizome and chrysanthemum into the wolfberry fruit, mixing, grinding into powder according to the mass ratio of 10.

Example 2

A preparation method of a processing method for enhancing the effect of barbary wolfberry fruit in treating eye diseases specifically comprises the following steps:

preparing four equal parts of wolfberry, adding yellow wine into each part of wolfberry, wherein the mass ratio of the wolfberry to the yellow wine is 8;

then grinding fructus Lycii into powder, extracting radix rehmanniae, radix rehmanniae Preparata, poria, atractylodis rhizoma, and flos Chrysanthemi with ten times of water for 2 hr for the first time, filtering to obtain extractive solution, extracting with eight times of water for the second time for 1 hr, filtering to obtain extractive solution, discarding residue, mixing the first and second extractive solutions, and concentrating the extractive solution. Mixing the wolfberry powder into the concentrated extract, granulating, and encapsulating or tabletting, wherein the mass ratio of the wolfberry, the radix rehmanniae, the prepared rehmannia root, the white poria, the white atractylodes rhizome and the chrysanthemum is 10.

Comparative example 1

Fructus Lycii without any treatment.

Comparative example 2

Parching fructus Lycii.

Comparative example 3

Soaking fructus Lycii in 5 times of yellow wine, and parching.

Detection of

1.4 kinds of parched Lycii Frutus obtained in example 1 and Lycii Frutus obtained in comparative examples 1-3 were pulverized and examined by gas chromatography-mass spectrometry (GC-MS) combination:

1) An experimental instrument:

GC 5977A (Agilent) -MS 7890B (Agilent), capillary chromatography DB-WAX (0.32 mm. Times.30m, 0.25), simultaneous distillation extractor

2) Experimental methods

Weighing 7 equal parts of a wolfberry powder sample, each part being 20g, adding the wolfberry powder sample into a 1000mL round-bottom flask on the water extraction side of a simultaneous distillation extraction instrument, adding 40g of sodium chloride and 350mL of distilled water, adding glass beads to prevent bumping, and heating by using an electric heating sleeve; adding 40mL of dichloromethane into a 250mL round-bottom flask on the organic solution extraction side of an instrument, heating by using an electrothermal sleeve, distilling and extracting for 2h at the same time, collecting a dichloromethane extracting solution, adding 2g of anhydrous sodium sulfate into the dichloromethane extracting solution for dehydration, standing overnight, and analyzing by using GC-MS;

GC-MS chromatographic mass spectrometry analysis conditions: temperature programming: the column temperature is 50 ℃, the temperature is kept for 1min, the temperature is raised to 250 ℃ at the speed of 3 ℃/min, the carrier gas is helium, the flow rate is 1mL/min, and the sample injection amount is 1 muL. Mass spectrum conditions: the ion source is an EI source, the temperature of the ion source is 250 ℃, the temperature of the injection port is 250 ℃, the pressure is 7.7psi, and the purging flow of the spacer is 3mL.

3) Analysis results

As shown in GC-MS analysis spectra of different samples in FIGS. 1-7, the GC-MS instrument is used to analyze the different components of processed fructus Lycii and original fructus Lycii, and the different components are compared with compounds in NIST database to identify that there are 2 different components in parched fructus Lycii; the stir-fried medlar after the yellow wine infiltration has 9 different components; the fructus lycii fried with pepper has 50 different components, wherein the different components mainly comprise hydrocarbons, terpenes, esters, ketones and the like; the mint-fried medlar has 26 different components, and the different components mainly comprise esters, ketones, alcohols, hydrocarbons and derivatives thereof; fructus Lycii parched with fructus Foeniculi has 13 different components, which mainly comprise ketones, aldehydes, alcohols, etc.; the black sesame fried medlar has 2 different components, which shows that the components of the processed medlar are changed compared with the components of the original medlar;

the identified different components are compared with yellow wine, pepper, mint, fennel and black sesame components reported in the prior literature, and butyric acid and n-hexanoic acid in the different components are volatile components in the yellow wine; limonene, anethole, (-) -4-terpineol, lauric acid and palmitic acid are volatile components contained in the pepper; the pulegone, the alpha-terpineol, the beta-bourbonene, the furfural and the eugenol are volatile components contained in the mint, and show that the volatile components in the processing auxiliary materials are increased in the wolfberry fruit processed under the proper processing conditions. The volatile components have the functions of ascending and dispersing and are beneficial to guiding the medicine upwards, thereby enhancing the efficacy of treating the eye diseases in the medicine.

2. Crushing the 4 fried medlar obtained in the example 1 and the medlar obtained in the comparative examples 1 to 3, and detecting the nonvolatile component change of the medlar fried with the aromatic medicament and the original medlar by adopting an ultra high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) technology:

1) Laboratory apparatus

An Agilent UHPLC-Q-TOF 6545+ system (equipped with electrospray ionization source ESI); data acquisition software: agilentMassHunter;

2) Experimental methods

Precisely weighing about 2.5g of each of 7 samples in a conical flask with a plug, precisely adding 25ml of methanol, carrying out ultrasonic treatment for 30min, standing to room temperature, weighing, complementing the lost weight, shaking up, standing, and analyzing by using ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS);

UHPLC-Q-TOF-MS chromatographic mass spectrometry analysis conditions are as follows: chromatographic conditions are as follows: using an Agilent T3 reverse phase chromatography column (2.1 mm. Times.100mm, 1.8 μm), mobile phase A (0.1% formic acid water) -B (0.1% formic acid acetonitrile), the gradient elution procedure is shown in the following table; the flow rate is 0.2mL/min, the column temperature is 30 ℃, and the injection volume is 1 muL. Mass spectrum conditions: the Q-TOF-MS system uses an ESI ion source to collect data in a positive ion mode, the scanning range is 100-1000 m/z, the temperature of Drying Gas (Gas Temp) is 320 ℃, and the flow rate of Drying Gas (Drying Gas) is 8L/min; atomizer pressure (Nebulizer) 35psig.

3) Results of the experiment

The experimental results are shown in fig. 8-14 below, and the processed wolfberry components of the 6 processed wolfberry samples all changed compared with the original wolfberry, wherein the change of the components of the pepper fried wolfberry sample is most obvious when the Retention Time (RT) is about 26min, and the change of the components of the fennel fried wolfberry and the mint fried wolfberry when the Retention Time (RT) is about 18min is more obvious.

3. Pharmacodynamic experiment of fructus Lycii formulation for treating ocular diseases in example 1

1) Laboratory animal

24 SPF SD male rats with the weight of 100-150g provided by Ningxia medical university animal center are raised in a clean environment;

2) Drugs and reagents

Normal saline, sodium iodate and eyeball fixative

3) Experimental methods

Modeling and administration of retinal injury: feeding 24 SD rats adaptively for one week, weighing, numbering, randomly dividing into 4 groups of 6 rats each, and dividing into control group, model group, unprocessed fructus Lycii prescription group, and processed fructus Lycii prescription group;

establishing a sodium iodate-induced SD rat retinal injury model, uniformly injecting normal saline into the tail vein of a control group, and uniformly injecting a sodium iodate solution (20 g/mL) into the tail vein of other groups at a ratio of 50mg/kg; starting from the first day after molding, the control group and the model group are both filled with stomach normal saline, the administration group is filled with 1.4mL/100g of whole powder (0.7 g/mL) with corresponding dosage, and the living condition of the rat is observed once a day. The eyeball of the rat is taken in the fourteenth day after the molding and is put into the eyeball fixing liquid for fixing for more than 24 hours. The eyeball is taken out and is subjected to sagittal incision, but the retina is not separated; fixing in fixing solution for one day, dehydrating, embedding in paraffin, slicing, and observing by HE staining;

4) Evaluation of pharmacodynamics

NaIO ₃ As a stable strong oxidant, can induce oxidative stress in the retina of various mammals, and specifically produce retinal toxicity, naIO ₃ It causes degeneration of retinal pigment epithelial cells (RPE), cones and rods, eventually leading to RPE cell necrosis, choroidal capillary atrophy, complete fusion of the Outer Nuclear Layer (ONL) and RPE layer, and panretinal degeneration. The damage to the retina is concentration-dependent and time-dependent, and the damage is more serious when the model is made earlier and the damage is more serious when the model is made more quickly.

The experimental results are shown in fig. 15, and the retina of the SD rat in the control group has complete structures of the retinal pigment epithelial cell (RPE) layer, photoreceptor cell inner and outer segment layer (IS/OS), inner Nuclear Layer (INL), outer Nuclear Layer (ONL), ganglion Cell Layer (GCL) and other layers, clear boundaries, and regularly and densely arranged cells. Compared with the control group, the structures of all layers of the retinas of the model group are changed to different degrees, wherein the thicknesses of ONL and INL layers of the retinas of rats are thinned, the structures are wavy, the boundaries of all layers are fuzzy, the fuzzy boundary of an Outer Plexiform Layer (OPL) is obvious, the gap between a choroid layer and an RPE layer is increased, the cells are disorganized, and part of the cells are lost. Compared with the model group, the rat retinas of the unprocessed Chinese wolfberry fruit formula group and the processed Chinese wolfberry fruit formula group are improved, but the inner nuclear layer cells of the unprocessed Chinese wolfberry fruit formula group are loose, the retinal recovery degree is slightly different from that of the processed Chinese wolfberry fruit formula group, and the rat retinas of the processed Chinese wolfberry fruit formula group are better in recovery degree.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A processing method for enhancing the effect of barbary wolfberry fruit in treating eye diseases is characterized by comprising the following steps: 4 equal parts of medlar are respectively fried with pepper, sesame, mint and fennel, and then the medlar is screened out and mixed evenly.

2. The processing method for enhancing medlar internal treatment of eye diseases according to claim 1, wherein the mass ratio of medlar to pepper, sesame, mint and fennel is 5-10.

3. The processing method of improving fructus lycii internal treatment eye disease efficacy according to claim 1, wherein the fructus lycii is soaked with yellow wine before being fried until the yellow wine is sucked dry.

4. The processing method for enhancing the effect of barbary wolfberry fruit on treating ocular diseases according to claim 3, wherein the mass ratio of the barbary wolfberry fruit to the yellow wine is 5-15.

5. The processing method of enhancing effects of fructus Lycii on ocular diseases as claimed in claim 1, wherein the parching is carried out to radix et rhizoma Rhei.

6. The processed Lycii Frutus obtained by the process according to any one of claims 1 to 5.

7. The use of wolfberry as claimed in claim 6 in the manufacture of a medicament for the prevention or treatment of an ocular disease.

8. The use of claim 7, wherein the medicament further comprises rehmannia glutinosa, rehmanniae radix Preparata, poria cocos, atractylodes macrocephala and Chrysanthemum morifolium.

9. The use of claim 8, wherein the mass ratio of wolfberry fruit, rehmannia glutinosa, prepared rehmannia glutinosa, poria cocos, atractylodes macrocephala and chrysanthemum is 5-15.

10. The use according to claim 7, wherein the medicament is in the form of any one of a pill, granule, tablet, powder and paste.