CN115316490A - Preparation method for fermenting bacillus coagulans feed by using wheat straws - Google Patents
Preparation method for fermenting bacillus coagulans feed by using wheat straws Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
- A23K10/28—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin from waste dairy products
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
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Abstract
A preparation method for fermenting bacillus coagulans feed by utilizing wheat straws comprises the steps of strain activation, liquid seed culture medium preparation, propagation inoculation, solid fermentation medium preparation, fermentation inoculation, pretreatment, feed preparation and the like, wherein solid fermentation is adopted in the fermentation process, the operation is simpler, and the fermented feed can be put into use only by simple drying and crushing; the wheat straw is adopted for solid-state fermentation, the spore rate and the cell number of bacillus coagulans in the fermented feed are guaranteed, meanwhile, only one kind of bacillus coagulans is added into the fermented feed, the spore rate and the cell number of the bacillus coagulans in the obtained fermented feed are high, on one hand, the effective utilization of the wheat straw is achieved, on the other hand, the fermented feed enables sufficient bacillus coagulans to be provided for animals, the intestinal environment of the animals is adjusted, and the effective utilization of the straw is practically achieved.
Description
Technical Field
The invention relates to the technical field of microbial fermentation feed, in particular to a preparation method for fermenting bacillus coagulans feed by using wheat straws.
Background
The straw is usually used as animal food for livestock breeding due to large resource reserve, the investment is low, the ecology can be better protected, the reasonable utilization of resources is realized, but in many areas, the utilization quality and the efficiency of the straw are relatively low, the most common straw feed at present is usually used as silage, the silage is formed by simple chopping, filling, compacting and sealing and fermenting, on one hand, the acidity of the silage is large, the feeding amount needs to be accurately controlled, on the other hand, the silage cannot provide other nutrients required by animals, and the effective utilization of the straw cannot be realized.
Chinese patent CN201810914381.2 (hereinafter referred to as comparison document) discloses a fermented feed produced by microbial treatment blasting crop straws and a production method and application thereof, wherein the production method part discloses that the fermented feed comprises steam blasting, mixing the blasted straws with water, adding fermentation bacteria liquid of bacillus coagulans and aspergillus oryzae into a mixture, and finally obtaining the fermented feed under specific conditions, wherein the steam blasting mainly destroys cellulose structures in the straws and assists in microbial degradation, the comparison document adopts the bacillus coagulans and the aspergillus oryzae to carry out synchronous fermentation, and controls the volume ratio of the bacillus coagulans and the aspergillus oryzae to generate synergistic action, so that the degradation effect of crude fibers is improved.
However, the fermented feed obtained by the method of the reference only aims to improve the degradation rate of crude fibers, so that the spore rate and the cell number of bacillus coagulans are low, and the bacillus coagulans is difficult to exist in intestinal tracts under natural conditions due to weak adhesion of epithelial cells in the intestinal tracts, so that the fermented feed produced by the method of the reference cannot provide sufficient bacillus coagulans for animals to adjust the intestinal tract environment of the animals.
Disclosure of Invention
In order to solve the technical problems in the background art and improve the utilization rate of the straws, the invention provides a preparation method for fermenting bacillus coagulans feed by using wheat straws.
The technical scheme of the invention is as follows:
a preparation method for fermenting Bacillus coagulans feed by using wheat straws comprises the following steps:
(1) And activating strains: activating bacillus coagulans preserved in a refrigerator for a long time;
(2) Preparing a liquid seed culture medium: in order to ensure the activation rate and the culture efficiency of strains, the liquid seed culture medium comprises 6-10% of glucose, 5-8% of whey powder, 6-10% of peptone, 6-10% of yeast powder, 1-3% of calcium carbonate, 0.2-0.5% of magnesium sulfate, 0.01-0.1% of manganese sulfate and 2-4% of dipotassium hydrogen sulfate;
(3) Expanding culture and inoculation: inoculating the slant strains (i.e. primary seeds) activated in the step (1) into a triangular flask containing the liquid seed culture medium in the step (2) for culture, transferring the strains cultured in the triangular flask to a 100L liquid fermentation tank for propagation, and gradually amplifying and propagating the slant strains from the triangular flask to the fermentation tank;
(4) Preparing a solid fermentation medium: the solid fermentation medium comprises water, wheat straws, bran and bean pulp, wherein compared with other straws, the wheat straws have good water retention and air permeability and are beneficial to strain fermentation, the bran and the bean pulp provide main carbon sources and nitrogen sources for fermentation, and a solid fermentation agent is subjected to sterilization treatment;
(5) And fermentation inoculation: inoculating the strain expanded in the step (3) into the solid fermentation medium in the step (4) to ferment until spores are formed, and preparing a fermentation material, wherein the way of only inoculating the bacillus coagulans into the fermentation material enables the spore rate and the cell number of the finally obtained bacillus coagulans of the fermented feed to be higher, so that sufficient bacillus coagulans are provided for animals, the intestinal environment is improved, and the activity of intestinal flora is improved;
(6) And (3) pretreatment: drying the fermentation material prepared in the step (5);
(7) And preparing the feed: and crushing the dried fermented material to prepare the feed.
In the preparation method for fermenting the bacillus coagulans feed by using the wheat straws, in the step (4), in order to ensure the spore rate of bacillus coagulans in the fermented feed, the ratio of the wheat straws, the bran and the bean pulp is 1.
Further, in the step (4), after the wheat straws, the wheat bran and the soybean meal are mixed according to a ratio of 1.
Further, the ratio of the material to the water of the mixture in the step (4) is 5, when the water amount is too much, the water content of the finally prepared fermentation material is higher, which is not beneficial to subsequent treatment, and when the water amount is too little, on one hand, the fermentation of the strains is not beneficial, and on the other hand, the sterilization effect of the solid fermentation medium is affected.
Further, after the feed water is mixed to prepare a mixture in the step (4), the humidity is controlled to be 45-55%, the temperature is 121 ℃, and the mixture is steamed and boiled for 20-30min for sterilization, because the temperature in the sterilization process is higher, the solid fermentation medium loses water, so that the water amount is reduced, and the solid fermentation medium after losing water is not beneficial to spore production of thalli, so the humidity needs to be controlled.
According to the preparation method for fermenting the bacillus coagulans feed by using the wheat straws, the pH value of the liquid seed culture medium in the step (2) is 7.0-7.5.
The preparation method for fermenting the bacillus coagulans feed by using the wheat straw comprises the following steps of (3):
inoculating the activated strain in the step (1) into a triangular flask containing the liquid seed culture medium in the step (2), and culturing for 16h at the temperature of 40 ℃ at 200 r/min;
transferring the strain cultured in the triangular flask into a liquid fermentation tank, and fermenting for 16-20h under the conditions of 40 ℃, 200r/min and the aeration ratio of 1.
According to the preparation method for fermenting the bacillus coagulans feed by using the wheat straws, after the inoculation in the step (5) is completed, aerobic fermentation is carried out for 40-48h at 43 ℃, so that the spore rate and the cell number of the bacillus coagulans are ensured.
The preparation method for fermenting the bacillus coagulans feed by using the wheat straw further comprises the step (8):
the method comprises the steps of pouring the feed into a flat plate for counting, measuring the spore rate, and measuring the index of the fermentation effect by using the spore rate, so that the fermentation effect can be more intuitively reflected when being detected.
The invention has the beneficial effects that:
the invention provides a preparation method for fermenting bacillus coagulans feed by utilizing wheat straws, wherein solid-state fermentation is adopted in the fermentation process, so that the operation is simpler, the energy consumption is lower, the water content of the fermented feed is smaller, and the fermented feed can be put into use only by simple drying and crushing;
the wheat straw is adopted for fermentation, and the wheat straw is suitable for growth of the bacillus coagulans due to strong air permeability and high fiber content, so that the spore rate and the cell number of the bacillus coagulans in the solid-state fermented feed are ensured;
according to the invention, a solid state fermentation mode is adopted, the spore rate of Bacillus coagulans is ensured, meanwhile, only one kind of Bacillus coagulans is added into the fermented feed, the spore rate and the cell number of the Bacillus coagulans in the obtained fermented feed are both high, on one hand, the effective utilization of wheat straws is achieved, on the other hand, sufficient Bacillus coagulans can be provided for animals when the fermented feed is used, so that the intestinal environment of the animals is adjusted, and the effective utilization of the straws is practically achieved;
the wheat straw, the wheat bran and the bean pulp are used as main raw materials of the solid-state fermentation medium, on one hand, the wheat straw, the wheat bran and the bean pulp are added to provide good nutrition for the growth of animals, and simultaneously provide sufficient carbon source and nitrogen source for the fermentation of the bacillus coagulans, and if the water, the wheat bran and the bean pulp are mixed, the density of the mixture is very high, which is not beneficial to the fermentation effect of the bacillus coagulans, so that the wheat straw with strong air permeability is specially added in the solid-state fermentation medium, the mixture is ensured to have good air permeability by adding the wheat straw into the mixture, and simultaneously the fiber content of the wheat straw is also high, which also provides a more beneficial environment for the growth of the bacillus coagulans, and ensures the spore rate and the cell number of the bacillus coagulans in the solid-state fermentation feed; moreover, the applicant specially designs the proportion of the wheat straw, the wheat bran and the soybean meal as 1.
Drawings
The aspects and advantages of the present application will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention.
In the drawings:
FIG. 1 is a schematic diagram of the preparation process of the feed in the example;
FIG. 2 is a graph showing a comparison of test results of each test group in test example 1;
FIG. 3 is a graph showing the results of tests in each test group in test example 2.
Detailed Description
Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings.
Example 1
The embodiment provides a preparation method for fermenting bacillus coagulans feed by using wheat straws, which comprises the following steps:
(1) And activating strains: transferring the bacillus coagulans slant test tube preserved in a refrigerator for a long time into a fresh slant culture medium, and standing and culturing at 40 ℃ for three days to obtain first-grade seeds;
(2) Preparing a liquid seed culture medium: in order to ensure the activation rate and the culture efficiency of strains, the liquid seed culture medium comprises 6-10% of glucose, 5-8% of whey powder, 6-10% of peptone, 6-10% of yeast powder, 1-3% of calcium carbonate, 0.2-0.5% of magnesium sulfate, 0.01-0.1% of manganese sulfate, 2-4% of dipotassium hydrogen sulfate and the pH value of the liquid seed culture medium is 7.0-7.5 (the components of the slant culture medium are the same as those of the liquid seed culture medium);
(3) And expanding culture and inoculation: selecting lawn (namely the first-stage seeds) from the inclined plane of the inclined plane culture medium in the step (1), transferring the lawn (namely the first-stage seeds) into a triangular flask containing a liquid seed culture medium, culturing for 16h at 40 ℃ at 200r/min, transferring the strain cultured in the triangular flask into a 100L liquid fermentation tank for propagation under the propagation conditions of 40 ℃, 200r/min, the aeration ratio of 1;
(4) And preparing a solid fermentation medium: 20 parts of wheat straw, 40 parts of bran, 40 parts of soybean meal, 3 parts of whey powder, 1 part of monopotassium phosphate and 1 part of calcium carbonate are adopted, on one hand, the calcium carbonate can be used for neutralizing, fermenting and producing acid, on the other hand, spore formation can be induced, clear water is added according to the proportion of 5 to 4, the humidity is controlled to be about 50 percent, the temperature is 121 ℃, and the wheat straw is cooled after being steamed, sterilized and cooled for 20-30 min;
(5) And fermentation inoculation: inoculating the seed liquid expanded in the step (3) into a solid fermentation medium according to the inoculation amount of 15%, and carrying out aerobic fermentation at 43 ℃ for 40-48h until spores are formed to prepare a fermentation material;
(6) And (3) pretreatment: drying the fermentation material prepared in the step (5);
(7) And preparing the feed: crushing the dried fermented material to prepare feed A (the feed A is a final product);
(8) The feed was counted by pour plate to give 8.9 x 10 9 cfu/g, water bath at 80 deg.C for 20min, and count result is 7.6 x 10 9 cfu/g, spore rate 85.4%.
Example 2
The embodiment provides a preparation method for fermenting bacillus coagulans feed by using wheat straws, which comprises the following steps:
(1) And activating strains: transferring the bacillus coagulans slant test tube preserved in a refrigerator for a long time into a fresh slant culture medium, and standing and culturing at 40 ℃ for three days to obtain first-grade seeds;
(2) Preparing a liquid seed culture medium: in order to ensure the activation rate and the culture efficiency of strains, the liquid seed culture medium comprises 6-10% of glucose, 5-8% of whey powder, 6-10% of peptone, 6-10% of yeast powder, 1-3% of calcium carbonate, 0.2-0.5% of magnesium sulfate, 0.01-0.1% of manganese sulfate, 2-4% of dipotassium hydrogen sulfate, and the pH value of the liquid seed culture medium is 7.0-7.5 (the components of the slant culture medium are the same as those of the liquid seed culture medium);
(3) And expanding culture and inoculation: selecting lawn (namely the first-stage seeds) from the inclined plane of the inclined plane culture medium in the step (1), transferring the lawn (namely the first-stage seeds) into a triangular flask containing a liquid seed culture medium, culturing for 16h at 40 ℃ at 200r/min, transferring the strain cultured in the triangular flask into a 100L liquid fermentation tank for propagation under the propagation conditions of 40 ℃, 200r/min, the aeration ratio of 1;
(4) And preparing a solid fermentation medium: 30 parts of wheat straw, 30 parts of bran, 30 parts of soybean meal, 3 parts of whey powder, 1 part of monopotassium phosphate and 1 part of calcium carbonate are adopted, on one hand, the calcium carbonate can be used for neutralizing, fermenting and producing acid, on the other hand, spore formation can be induced, clear water is added according to the proportion of 5 to 4, the humidity is controlled to be about 50 percent, the temperature is 121 ℃, and the wheat straw is cooled after being steamed, sterilized and cooled for 20-30 min;
(5) And fermentation inoculation: inoculating the seed liquid expanded in the step (3) into a solid fermentation medium according to the inoculation amount of 15%, and carrying out aerobic fermentation at 43 ℃ for 40-48h until spores are formed to prepare a fermentation material;
(6) And (3) pretreatment: drying the fermentation material prepared in the step (5);
(7) And preparing the feed: crushing the dried fermented material to obtain feed B (the feed B is a final product);
(8) The feed was counted by pour plate to give 9.5 x 10 9 cfu/g, 8.5 x 10 after 20min in 80 ℃ water bath 9 cfu/g, spore rate 89.5%.
Comparative example 1
The comparative example provides a preparation method for fermenting bacillus coagulans feed by using wheat straws, which comprises the following steps:
(1) And activating strains: transferring the bacillus coagulans slant test tube preserved in a refrigerator for a long time into a fresh slant culture medium, standing and culturing for three days at 40 ℃ to obtain first-grade seeds;
(2) Preparing a liquid seed culture medium: in order to ensure the activation rate and the culture efficiency of strains, the liquid seed culture medium comprises 6-10% of glucose, 5-8% of whey powder, 6-10% of peptone, 6-10% of yeast powder, 1-3% of calcium carbonate, 0.2-0.5% of magnesium sulfate, 0.01-0.1% of manganese sulfate, 2-4% of dipotassium hydrogen sulfate, and the pH value of the liquid seed culture medium is 7.0-7.5 (the components of the slant culture medium are the same as those of the liquid seed culture medium);
(3) And expanding culture and inoculation: selecting lawn (namely the first-stage seeds) from the inclined plane of the inclined plane culture medium in the step (1), transferring the lawn to a triangular flask containing a liquid seed culture medium, and culturing for 16h at the temperature of 40 ℃ at 200 r/min;
transferring the strain cultured in the triangular flask to a 100L liquid fermentation tank for propagation under the conditions of 40 ℃, 200r/min and the aeration ratio of 1;
(4) And preparing a solid fermentation medium: adopting 20 parts of rice hulls, 40 parts of bran, 40 parts of soybean meal, 3 parts of whey powder, 1 part of monopotassium phosphate and 1 part of calcium carbonate, on one hand, the calcium carbonate can be used for neutralizing, fermenting and producing acid, on the other hand, the calcium carbonate can induce spore formation, adding clear water according to the proportion of 5 to 4, controlling the humidity to be about 50 percent, controlling the temperature to be 121 ℃, and cooling after cooking and sterilizing for 20-30 min;
(5) And fermentation inoculation: inoculating the seed solution expanded in the step (3) into a solid fermentation medium according to the inoculation amount of 15%, and performing aerobic fermentation at 43 ℃ for 40-48h until spores are formed to prepare a fermentation material;
(6) And (3) pretreatment: drying the fermentation material prepared in the step (5);
(7) And preparing the feed: crushing the dried fermented material to prepare feed C (the feed C is a final product);
(8) The feed was counted by pouring on a plate and the result was 9.6 x 10 9 cfu/g, water bath at 80 ℃ for 20min, and count result of 5.8 x 10 9 cfu/g, the spore rate is 60.4%.
Test example 1
(1) And randomly dividing 240 broiler chicks with basically consistent initial weights into four groups, wherein each group comprises 60 broiler chicks, and the groups are respectively a first implementation group, a second implementation group, a first control group and a second control group.
(2) Feeding the four groups of broiler chicks with basic ration consisting of 55% of corn, 35% of soybean meal, 1% of vegetable oil, 0.5% of stone powder and 2% of compound premix;
(3) The first embodiment group adds 10% of feed A to the basic ration, the second embodiment group adds 10% of feed B to the basic ration, and the first control group adds 10% of feed C to the basic ration;
(4) Free feeding and drinking of broiler chicks, and routine management of daily cleaning, disinfection and epidemic prevention.
(5) Recording the feed intake of the four groups of broiler chicks by taking the weight as a unit every day, respectively weighing the broiler chicks on an empty stomach at 0d, 21d and 42d, and calculating the average daily gain, the average daily feed intake and the feed-weight ratio;
(6) And (5) after the step (5) is finished, randomly selecting one broiler chicken for test in each group, dissolving 0.5g of intestinal contents in 4.5mL of physiological saline water, diluting by a 10-time ratio, selecting a proper dilution gradient to measure the flora number, and measuring the lactobacillus number and the escherichia coli number in the intestinal contents of the four groups of broiler chickens by using a plate counting method.
Test example 2
(1) Selecting 120 broiler chicks with the initial weight basically consistent with that of the broiler chicks in the test example 1, randomly dividing the broiler chicks into two groups, wherein each group comprises 60 broiler chicks, and the two groups are respectively a third control group and a fourth control group;
(2) Feeding the two groups of broiler chicks with basic ration consisting of 55% of corn, 35% of soybean meal, 1% of vegetable oil, 0.5% of stone powder and 2% of compound premix;
(3) The third control group is added with 10% of feed B in the basic ration, the fourth control group is added with 10% of feed B in the basic ration, and the broiler chicks of the fourth control group stop feeding the basic ration containing 10% of feed B after 21d and only feed the common basic ration in the step (2);
(4) Free feeding and drinking of broiler chicks, and routine management of daily cleaning, disinfection and epidemic prevention modes;
(5) And recording the feed intake of two groups of broiler chicks by taking the weight as a unit every day, randomly selecting one broiler chicken for test in each group when the broiler chickens are respectively at the 0d, 21d and 42d, dissolving 0.5g of intestinal contents in 4.5mL of physiological saline, diluting by a 10-fold ratio, selecting a proper dilution gradient for determining the flora number, and determining the lactobacillus number and the escherichia coli number in the intestinal contents of the two groups of broiler chickens when the plate counting method is used for determining different days.
The application of the preparation method for fermenting the bacillus coagulans feed by utilizing the wheat straws can be seen from fig. 2 and fig. 3, the fermented feed produced by the production method has obvious effects of inhibiting the number of escherichia coli, increasing the number of intestinal lactic acid bacteria and improving the intestinal environment, and meanwhile, the spore rate of the bacillus coagulans can reach more than 60 percent by adopting the fermented feed produced by the production method, especially, the bacillus coagulans is fermented by adopting the wheat straws: bran: when the soybean meal is 1.
Claims (9)
1. A preparation method for fermenting Bacillus coagulans feed by using wheat straws is characterized by comprising the following steps:
(1) And activating strains: activating bacillus coagulans preserved in a refrigerator for a long time;
(2) Preparing a liquid seed culture medium: the liquid seed culture medium comprises 6-10% of glucose, 5-8% of whey powder, 6-10% of peptone, 6-10% of yeast powder, 1-3% of calcium carbonate, 0.2-0.5% of magnesium sulfate, 0.01-0.1% of manganese sulfate and 2-4% of dipotassium hydrogen sulfate;
(3) And expanding culture and inoculation: inoculating the activated strain in the step (1) into a triangular flask containing the liquid seed culture medium in the step (2) for culture, and transferring the strain cultured in the triangular flask into a liquid fermentation tank for propagation culture;
(4) Preparing a solid fermentation medium: the solid fermentation medium comprises water, wheat straws, bran and bean pulp, and the solid fermentation agent is subjected to sterilization treatment;
(5) And fermentation inoculation: inoculating the strain expanded in the step (3) into the solid fermentation medium in the step (4) to ferment until spores are formed, and preparing a fermentation material;
(6) And (3) pretreatment: drying the fermentation material prepared in the step (5);
(7) And preparing the feed: and crushing the dried fermented material to prepare the feed.
2. The method for preparing a bacillus coagulans feed by fermenting wheat straw according to claim 1, wherein in the step (4), the ratio of the wheat straw to the wheat bran to the soybean meal is 1.
3. The preparation method of the bacillus coagulans feed fermented by the wheat straw according to claim 2 is characterized in that in the step (4), the wheat straw, the bran and the soybean meal are mixed according to a ratio of 1.
4. The preparation method of the bacillus coagulans feed by utilizing the wheat straw for fermentation according to the claim 3, wherein the ratio of the mixture to the water in the mixture in the step (4) is 5.
5. The preparation method of the bacillus coagulans feed by utilizing the wheat straw for fermentation according to claim 3, wherein the mixture prepared by mixing the feed water in the step (4) is sterilized by steaming at 121 ℃ for 20-30min under the humidity of 40-55%.
6. The method for preparing a Bacillus coagulans feed by fermenting wheat straw as claimed in claim 1, wherein the pH of the liquid seed culture medium in step (2) is 7.0-7.5.
7. The preparation method of the bacillus coagulans feed by fermenting wheat straw according to claim 1, wherein the propagation inoculation method in the step (3) is as follows:
inoculating the activated strain in the step (1) into a triangular flask containing the liquid seed culture medium in the step (2), and culturing for 16h at the temperature of 40 ℃ at the speed of 200 r/min;
transferring the strain cultured in the triangular flask into a liquid fermentation tank, and fermenting for 16-20h under the conditions of 40 ℃, 200r/min and the aeration ratio of 1.
8. The method for preparing a bacillus coagulans feed by fermenting wheat straw according to claim 1, wherein the aerobic fermentation is carried out at 43 ℃ for 40-48h after the inoculation in the step (5) is completed.
9. The preparation method of the bacillus coagulans feed by utilizing the wheat straw for fermentation according to the claim 1, which is characterized by further comprising the step (8):
the feed was poured onto a plate for counting and the spore rate was measured.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104762250A (en) * | 2015-03-30 | 2015-07-08 | 华南师范大学 | Method for producing probiotics by utilizing lignocellulose hydrolysate |
| CN105567740A (en) * | 2016-01-27 | 2016-05-11 | 济南航晨生物科技有限公司 | High-density solid fermentation method for agricultural bacillus |
| CN108464388A (en) * | 2017-02-23 | 2018-08-31 | 丽水学院 | A method of solid state fermentation being carried out to wood fibre hydrolysis raw material using bacillus coagulans and produces fermented feed |
| CN108740297A (en) * | 2018-08-13 | 2018-11-06 | 齐齐哈尔大学 | The fermented feed and its production method of a kind of microbiological treatment explosion crop straws for producing and application |
-
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- 2022-08-19 CN CN202211000612.1A patent/CN115316490A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104762250A (en) * | 2015-03-30 | 2015-07-08 | 华南师范大学 | Method for producing probiotics by utilizing lignocellulose hydrolysate |
| CN105567740A (en) * | 2016-01-27 | 2016-05-11 | 济南航晨生物科技有限公司 | High-density solid fermentation method for agricultural bacillus |
| CN108464388A (en) * | 2017-02-23 | 2018-08-31 | 丽水学院 | A method of solid state fermentation being carried out to wood fibre hydrolysis raw material using bacillus coagulans and produces fermented feed |
| CN108740297A (en) * | 2018-08-13 | 2018-11-06 | 齐齐哈尔大学 | The fermented feed and its production method of a kind of microbiological treatment explosion crop straws for producing and application |
Non-Patent Citations (1)
| Title |
|---|
| 于佳民;张建梅;张文;谷巍;: "凝结芽孢杆菌产芽孢发酵条件的优化", 中国饲料, no. 08 * |
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