CN115286422A - Preparation method of selenium-rich ceramic teapot capable of flavoring tea water - Google Patents
Preparation method of selenium-rich ceramic teapot capable of flavoring tea water Download PDFInfo
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Abstract
The invention belongs to the field of new materials of domestic functional ceramics, and particularly relates to a preparation method of a selenium-rich ceramic teapot capable of flavoring tea water, which comprises the steps of uniformly mixing selenium-rich pottery clay and a pore-forming agent by mass percent, adding water, mixing to prepare ceramic pug, ageing, and preparing a green ceramic teapot blank by using the ceramic pug; drying and calcining the green ceramic teapot blank to obtain a porous ceramic teapot matrix; coating a layer of ceramic glaze on the outer surface of the porous ceramic teapot matrix, and then carrying out secondary firing to obtain the ceramic teapot; filling a ceramic teapot with a dilute acid solution, and activating the surface of the ceramic teapot at a certain temperature; filling the ceramic teapot in the step with a cross-linking agent solution, and then filling the dried ceramic teapot with the prepared enzyme solution for adsorption; and (3) filling a dopamine Tris-HCl buffer solution into the ceramic teapot after adsorption is finished, and soaking to strengthen the immobilization effect. According to the invention, the enzyme is fixed in the ceramic teapot by adopting an immobilized enzyme technology, and the prepared ceramic teapot has the tea aroma-enhancing effect.
Description
Technical Field
The invention belongs to the field of novel daily functional ceramic materials, and particularly relates to a preparation method of a selenium-rich ceramic teapot capable of flavoring tea water.
Background
The requirements of people on tea drinking modes and experiences are higher and higher, and tea water aroma enhancement is a main breakthrough direction of current tea drinks. At present, the related research on tea aroma enhancement is more, and the tea aroma enhancement is improved from the aspect of tea processing technology, for example, the invention patent "a method for enhancing aroma and removing bitter and astringent taste of black tea in summer and autumn" (CN 202010589606.9) discloses a set of processing technological process capable of enhancing aroma of black tea. For example, the invention patent (CN 201710254595.7) discloses a complex enzyme preparation for aroma enhancement of tea, which contains papain, beta-glucanase and flavor glycosidase in different proportions. The invention discloses an aroma-enhancing semi-fermented polygonum multiflorum leaf tea and a preparation method thereof (CN 201410113072.7), which adopts galactosidase to enhance the aroma of the black leaf tea. In the research on the aroma quality of green tea beverage by using immobilized enzyme technology (Lvlianmei, zhejiang university, 2004), chitosan, alginate and agar are used as embedding materials, and pectinase and beta-glucosidase are prepared into immobilized enzyme for improving the aroma of tea.
However, the above reports do not combine the immobilized enzyme technology, the ceramic teapot and the tea aroma-enhancing technology into a whole, so as to realize the tea aroma-enhancing type selenium-enriched ceramic teapot.
Disclosure of Invention
The invention aims to provide a preparation method of a selenium-enriched ceramic teapot capable of enhancing aroma of tea water, which combines ceramic materials science and an immobilized enzyme technology to fill the blank of the tea water aroma-enhancing type selenium-enriched ceramic teapot, and has the advantages of simple preparation process and easy adjustment.
The scheme adopted by the invention for realizing the purpose is as follows: a preparation method of a selenium-enriched ceramic teapot capable of flavoring tea water comprises the following steps:
(1) According to the mass percent, 75-85% of selenium-rich pottery clay and 15-25% of pore-forming agent are uniformly mixed to obtain a mixture, water accounting for 30-40% of the mass of the mixture is added, the mixture is kneaded to prepare ceramic pug, the ceramic pug is aged, and ceramic raw materials are adopted to prepare ceramic teapot green bodies;
(2) Drying the prepared green ceramic teapot body, and calcining to obtain a porous ceramic teapot body;
(3) Coating a layer of ceramic glaze on the outer surface of the obtained porous ceramic teapot matrix, then carrying out secondary firing, and cooling along with a furnace to obtain the ceramic teapot;
(4) Filling a ceramic teapot with a dilute acid solution, activating the ceramic surface at a certain temperature, washing and drying after activation;
(5) Filling the ceramic teapot obtained in the step (4) with a cross-linking agent solution, preserving heat for a certain time at a certain temperature, drying, and filling the dried ceramic teapot with the prepared enzyme solution for adsorption;
(6) And (3) filling a dopamine Tris-HCl buffer solution into the adsorbed ceramic teapot, soaking to enhance the immobilization effect, washing and drying to obtain the selenium-enriched ceramic teapot capable of enhancing the aroma of tea water.
Preferably, in the step (1), the selenium-rich pottery clay contains more than or equal to 0.4mg/kg of total selenium element, has an average particle size of 45-75 μm, and adopts graphite powder or starch as a pore-forming agent, and has an average particle size of 18-25 μm.
Preferably, in the step (2), the firing temperature schedule of the porous ceramic teapot matrix is as follows: the heating rate is 5-10 ℃/mi5, the highest temperature point is 1100-1160 ℃, the temperature is respectively kept at 300 ℃, 500 ℃ and 800 ℃ for 15-30 mi5, and the temperature is kept at 90-120 mi5.
Preferably, in the step (3), the ceramic glaze is coated by adopting a glaze dipping mode, and the formula of the glaze slip for glaze dipping comprises the following components in percentage by mass: 50 to 60 percent of borosilicate glass frit glaze, 40 to 50 percent of water and 0.5 to 1.0 percent of ammonium polyacrylate dispersant.
Preferably, in the step (3), the temperature system of the secondary firing is: the heating rate is 15-20 ℃/mi5, the highest temperature point is 980-1050 ℃, and the temperature is kept at the highest temperature by 20-30 mi5.
Preferably, in the step (4), the ceramic teapot is filled with a dilute hydrochloric acid solution with the mass fraction of 18-25% and then activated at 85-95 ℃ for 2-4 h.
Preferably, in the step (5), a cross-linking agent glutaraldehyde solution with the volume concentration of 1.5-2.0% is filled in the ceramic teapot, and then the ceramic teapot is dried after the ceramic teapot is reacted for 2-4 hours at the temperature of 80-90 ℃, wherein the drying temperature is 60-65 ℃.
Preferably, in the step (5), the enzyme solution is prepared by adopting 45-55 mmol/L citric acid-disodium hydrogen phosphate buffer solution to obtain 200-250U/mL beta-glucosidase and beta-primeveroside with the mass ratio of 1.
Preferably, in the step (5), the specific operation of adsorption is that the dried teapot is filled with enzyme solution, and the teapot is vacuumized for 1 to 2 hours under the vacuum degree of 85 to 95 kPa; after the vacuum pumping is finished, adsorbing for 5-8 h at the temperature of 2-5 ℃, and repeating the adsorption process for 3-6 times until the adsorption is finished.
Preferably, in the step (6), the ceramic teapot is filled with 1.6-2.4 mg/mL dopamine Tris-HCl buffer solution and soaked for 12-16 h under the vacuum degree of 85-95 kPa to strengthen the enzyme immobilization effect; finally, the mixture is washed by a citric acid-disodium hydrogen phosphate buffer solution with the concentration of 45 to 55mmol/L and dried at the temperature of between 55 and 65 ℃.
The invention has the following advantages and beneficial effects:
(1) The ceramic teapot prepared by the invention has excellent tea fragrance-enhancing effect and is suitable for soaking all green tea. Beta-glucosidase and beta-primeveroside are fixed in a ceramic teapot by adopting an immobilized enzyme technology, so that the concentration of aroma substances in tea water can be increased by about 30-36% (measured by a gas chromatography-mass spectrometer).
(2) The tea aroma-enhancing ceramic teapot prepared by the invention also has a health-preserving function of releasing selenium element. By adopting the selenium-rich pottery clay to prepare the ceramic teapot, selenium can be released into tea water in the tea making process. Meanwhile, the ceramic teapot designed and prepared by the invention is internally provided with rich porous structures with high specific surface area, so that the immobilized enzyme is facilitated, the release efficiency of selenium element can be improved, and the selenium content in the tea water is measured to be more than or equal to 0.004ppm by a hydride-atomic fluorescence spectrometer. Selenium element is beneficial to human bodies, so that the teapot prepared by the invention has a health preserving function.
Drawings
FIG. 1 is a schematic structural view of a ceramic teapot made in accordance with the present invention;
FIG. 2 is an SEM image of the porous ceramic matrix prepared in example 1.
In the figure: 1. a kettle handle; 2. a kettle cover; 3. a spout; 4. a kettle body; 5. a porous ceramic matrix of immobilized enzyme; 6. compact glaze shell.
Detailed Description
The following examples are provided to further illustrate the present invention for better understanding, but the present invention is not limited to the following examples.
Example 1:
a preparation method of a selenium-rich ceramic teapot capable of flavoring tea water comprises the following steps:
the first step of the preparation of the ceramic teapot:
(1) Raw materials and mixing materials: according to the mass percentage, 80 percent of pottery clay (selenium-rich pottery clay with the content of total selenium element of 0.52mg/kg and the average particle size of 62 mu m) and 20 percent of graphite powder (the average particle size of 22 mu m) are taken and are ball-milled and mixed evenly to obtain a mixture;
(2) Pugging and aging: adding water accounting for 30% of the mass of the mixture, mixing for 6 hours by using a pug mill to prepare ceramic pug, and sealing and ageing for 64 hours by using a freshness protection package;
(3) Preparing a green ceramic teapot by plastic forming: beating the ceramic pug in the step (2) into mud pieces and mud strips with uniform thickness; manually manufacturing and assembling a kettle body, a kettle mouth, a kettle cover and a kettle handle of the ceramic teapot;
(4) Drying and firing: drying the ceramic green body in the step (3) in a drying oven at 100 ℃ for 36h; after drying, placing the ceramic green body in a muffle furnace to be sintered into a porous ceramic teapot matrix; the firing temperature system of the porous ceramic teapot matrix is as follows: the heating rate is 8 ℃/mi5, the highest temperature point is 1120 ℃, the temperature is respectively kept at 300 ℃, 500 and 800 ℃ for 20mi5, and the temperature is kept at the highest temperature point for 100mi5; as shown in FIG. 2, the SEM image of the prepared porous ceramic teapot matrix shows that the abundant pore structure in the ceramic teapot matrix can be seen, and the subsequent immobilized enzyme can play a role of providing a loading space.
(5) Glazing the surface of the teapot: applying a layer of ceramic glaze on the outer surface of the porous ceramic teapot matrix obtained in the step (4) in a glaze dipping mode; the formula of the glaze slip for dipping glaze comprises the following components in percentage by mass: 55% of borosilicate glass frits glaze, 45% of water, and 0.8% of ammonium polyacrylate dispersant. The glaze dipping process adopts a dipping-pulling method, the outer surface of the ceramic teapot body is dipped into glaze slip, extraction is carried out after the glaze slip is kept for 8 seconds, and the extraction is repeated for 5 times.
(6) Secondary sintering: and (4) sintering the glazed ceramic teapot again in a muffle furnace, and cooling along with the furnace to obtain the ceramic teapot. The temperature system of the secondary sintering is as follows: the heating rate is 18 ℃/mi5, the maximum temperature point is 1020 ℃, and the temperature is kept at 25mi5.
And (2) second step of ceramic teapot immobilized enzyme:
(1) Preparation of enzyme solution: preparing an enzyme solution with the concentration of 225U/mL by adopting a 50mmol/L citric acid-disodium hydrogen phosphate buffer solution for later use; the enzyme is beta-glucosidase or beta-primrose glucosidase;
(2) Pretreating the ceramic teapot: filling the prepared dilute hydrochloric acid solution with the mass fraction of 20% into a ceramic teapot to activate the surface of the ceramic, which is beneficial to enzyme immobilization: activating in 90 ℃ water bath for 3h, taking out, repeatedly cleaning with clear water for 8 times, and drying in an oven at 100-105 ℃ for 20h;
(3) Immobilized enzyme: filling the interior of the ceramic teapot obtained in the step (2) with a cross-linking agent glutaraldehyde solution with the volume concentration of 1.8%; reacting in water bath at 85 ℃ for 3h, and taking out; drying the taken ceramic teapot at 62 ℃ for 3h; filling the dried teapot with the enzyme solution prepared in the step (1), and vacuumizing for 1.5h under the vacuum degree of 90 kPa; after the evacuation was completed, the mixture was adsorbed in a refrigerator at 3 ℃ for 6 hours, and the adsorption process was repeated 5 times. Taking out the teapot after adsorption is finished, filling a dopamine Tris-HCl buffer solution with the concentration of 2.2mg/mL into the teapot, and soaking for 15 hours under the vacuum degree of 90kPa to strengthen the enzyme immobilization effect; and (3) finally, washing by adopting the buffer solution in the step (1), and drying at 60 ℃ to obtain the final selenium-enriched immobilized enzyme ceramic teapot for making tea. The temperature of the tea making water is not higher than 70 ℃.
Table 1 shows the increase rate of the tea aroma substance concentration at different ratios of two enzymes (β -glucosidase and β -primeverosidase).
TABLE 1 five different enzyme treatment ratios and corresponding green tea water aroma combination properties
Note: a is the concentration of beta-glucosidase, B is the concentration of beta-primeveroside; CK is no addition of any enzyme.
As can be seen from the data in table 1, compared with the ceramic teapot without immobilized enzyme, the concentration of the tea aroma substance in the teapot prepared when the concentration ratio of the beta-glucosidase to the beta-primeverosidase is 1; after 50 times of brewing, the aroma substance concentration still has 20.2 percent of improvement rate. It can be known that adding these two enzymes simultaneously can obviously improve the aroma-enhancing effect. In addition, since the selenium-rich ceramic is used, the selenium content in the tea water is measured to be 0.005ppm.
Example 2:
the first step of the preparation of the ceramic teapot:
(1) Raw materials and mixing materials: according to the mass percentage, 85 percent of pottery clay (selenium-rich pottery clay with the content of total selenium element of 0.40mg/kg and the average particle size of 45 mu m) and 15 percent of graphite powder (the average particle size of 18 mu m) are taken, ball-milled and mixed evenly to obtain a mixture;
(2) Pugging and aging: adding water accounting for 30% of the mixture, mixing for 5h by a pug mill to prepare ceramic pug, and sealing and aging for 48h by a freshness protection package;
(3) Preparing a green ceramic teapot by plastic forming: beating the ceramic pug in the step (2) into mud pieces and mud strips with uniform thickness; manually manufacturing and assembling a kettle body, a kettle mouth, a kettle cover and a kettle handle of the ceramic teapot;
(4) Drying and firing: drying the ceramic green body in the step (3) in a drying oven at 95 ℃ for 24 hours; after drying, placing the ceramic green body in a muffle furnace to be sintered into a porous ceramic teapot matrix; the firing temperature system of the porous ceramic teapot matrix is as follows: the heating rate is 5 ℃/mi5, the highest temperature point is 1100 ℃, the temperature is respectively kept at 300 ℃, 500 and 800 ℃ for 15mi5, and the temperature is kept at 90mi5;
(5) Glazing the surface of the teapot: applying a layer of ceramic glaze on the outer surface of the porous ceramic teapot matrix obtained in the step (4) in a glaze dipping mode; the formula of the glaze slip for dipping glaze comprises the following components in percentage by mass: 50% borosilicate glass frit glaze, 50% water, and 0.5% ammonium polyacrylate dispersant. Dipping the outer surface of the ceramic teapot body into glaze slip by adopting a dipping-pulling method in the glaze dipping process, extracting after keeping for 5 seconds, and repeating for 3 times;
(6) Secondary sintering: and (4) sintering the glazed ceramic teapot again in a muffle furnace, and cooling along with the muffle furnace to obtain the ceramic teapot. The temperature system of the secondary sintering is as follows: the heating rate is 15 ℃/mi5, the maximum temperature point is 980 ℃, and the temperature is kept at 20mi5.
And (2) second step of ceramic teapot immobilized enzyme:
(1) Preparation of enzyme solution: preparing enzyme solution with the concentration of 200U/mL by adopting 45mmol/L citric acid-disodium hydrogen phosphate buffer solution for later use; the enzyme is beta-glucosidase and beta-primeveroside, and the concentration ratio is 1;
(2) Pretreating the ceramic teapot: filling the prepared dilute hydrochloric acid solution with the mass fraction of 18% into a ceramic teapot to activate the surface of the ceramic, which is beneficial to enzyme immobilization: activating in 85 deg.C water bath for 2 hr, taking out, repeatedly cleaning with clear water for 5 times, and oven drying in 100 deg.C oven for 12 hr;
(3) Immobilized enzyme: filling the ceramic teapot obtained in the step (2) with cross-linking agent glutaraldehyde solution with the volume concentration of 1.5%; reacting in water bath at 80 ℃ for 2h, and taking out; drying the taken out ceramic teapot at 60 ℃ for 2h; filling the dried teapot with the enzyme solution prepared in the step (1), and vacuumizing for 1h under the vacuum degree of 85 kPa; after the evacuation was completed, the mixture was adsorbed in a refrigerator at 2 ℃ for 5 hours, and the adsorption process was repeated 3 times. Taking out the teapot after adsorption is finished, filling a dopamine Tris-HCl buffer solution with the concentration of 1.6mg/mL into the teapot, and soaking for 12 hours under the vacuum degree of 85kPa to strengthen the enzyme immobilization effect; and (3) finally, washing by adopting the buffer solution in the step (1), and drying at 55 ℃ to obtain the final selenium-enriched immobilized enzyme ceramic teapot for making tea, wherein the water temperature of the tea making water is 65 ℃.
The concentration of the tea aroma substances soaked by the teapot of the embodiment is improved by 32.2%; after 50 times of brewing, the aroma substance concentration increase rate is 18.9%; meanwhile, the selenium content in the tea water is 0.004ppm.
Example 3:
the first step of the preparation of the ceramic teapot:
(1) Raw materials and mixing: according to the mass percentage, 75 percent of pottery clay (selenium-rich pottery clay with the content of total selenium element of 0.60mg/kg and the average particle size of 75 mu m) and 25 percent of starch (the average particle size of 25 mu m) are taken, ball-milled and mixed evenly to obtain a mixture;
(2) Pugging and aging: adding water accounting for 40% of the mass of the mixture, mixing for 8 hours by using a pug mill to prepare ceramic pug, and sealing and ageing for 72 hours by using a freshness protection package;
(3) Preparing a green ceramic teapot blank by plastic forming: beating the ceramic pug in the step (2) into mud pieces and mud strips with uniform thickness; manually manufacturing and assembling a kettle body, a kettle mouth, a kettle cover and a kettle handle of the ceramic teapot;
(4) Drying and firing: drying the ceramic green body in the step (3) in a drying oven at 105 ℃ for 48 hours; after drying, placing the ceramic green body in a muffle furnace to be sintered into a porous ceramic teapot matrix; the firing temperature system of the porous ceramic teapot matrix is as follows: the heating rate is 10 ℃/mi5, the highest temperature point is 1160 ℃, the temperature is respectively kept at 300 ℃, 500 and 800 ℃ for 30mi5, and the temperature is kept at 120mi5;
(5) Glazing the surface of the teapot: applying a layer of ceramic glaze on the outer surface of the porous ceramic teapot matrix obtained in the step (4) in a glaze dipping mode; the formula of the glaze slip for dipping glaze comprises the following components in percentage by mass: 60% borosilicate glass frit glaze, 40% water, and 1.0% ammonium polyacrylate dispersant. Dipping the outer surface of the ceramic teapot matrix into the glaze slip by adopting a dipping-pulling method in the glaze dipping process, keeping for 10 seconds, then extracting, and repeating for 5 times;
(6) Secondary sintering: and (4) sintering the glazed ceramic teapot again in a muffle furnace, and cooling along with the muffle furnace to obtain the ceramic teapot. The temperature system of the secondary sintering is as follows: the heating rate is 20 ℃/mi5, the maximum temperature point is 1050 ℃, and the temperature is kept at 30mi5.
And (2) second step of ceramic teapot immobilized enzyme:
(1) Preparing an enzyme solution: preparing enzyme solution with the concentration of 250U/mL by using 55mmol/L citric acid-disodium hydrogen phosphate buffer solution for later use; the enzyme is beta-glucosidase and beta-primeveroside, and the concentration ratio is 1;
(2) Pretreating the ceramic teapot: filling a ceramic teapot with the prepared dilute hydrochloric acid solution with the mass fraction of 25% to activate the surface of the ceramic teapot, and facilitating enzyme immobilization: activating in 95 deg.C water bath for 4 hr, taking out, repeatedly washing with clear water for 10 times, and oven drying in 105 deg.C oven for 24 hr;
(3) Immobilized enzyme: filling a cross-linking agent glutaraldehyde solution with the volume concentration of 2.0% into the ceramic teapot obtained in the step (2); reacting in water bath at 90 ℃ for 4h, and taking out; drying the taken ceramic teapot at 65 ℃ for 4h; filling the dried teapot with the enzyme solution prepared in the step (1), and vacuumizing for 2h under the vacuum degree of 95 kPa; after the vacuum pumping, the mixture was adsorbed in a refrigerator at 5 ℃ for 8 hours, and the adsorption process was repeated 6 times. Taking out the teapot after adsorption is finished, filling dopamine Tris-HCl buffer solution with the concentration of 2.4mg/mL into the teapot, and soaking for 16 hours under the vacuum degree of 95kPa to strengthen the enzyme immobilization effect; and (3) finally, washing by adopting the buffer solution in the step (1), and drying at 65 ℃ to obtain the final selenium-enriched immobilized enzyme ceramic teapot for making tea, wherein the water temperature of the tea-making water is 70 ℃.
The tea water fragrance substance concentration of the teapot in the embodiment is improved by 34.8%; after 50 times of brewing, the aroma substance concentration increase rate is 19.8%; in addition, the selenium content in the tea water reaches 0.005ppm.
Comparative example 1
This comparative example differs from example 2 only in that: the vacuum pumping operation is not carried out in the enzyme liquid adsorption process, so that the concentration of the tea aroma substances soaked in the teapot of the comparative example is improved by 27.6 percent; after 50 times of brewing, the aroma substance concentration of the tea water is increased by 17.7 percent. As can be seen by comparison with the data of example 2, the increased fragrancing effect is achieved by applying a vacuum.
Comparative example 2
The difference between the comparative example and the example 3 is only that the dopamine buffer solution is not used for soaking after adsorption to strengthen the effect of immobilized enzyme, and the tea aroma substance concentration of the teapot soaked by the teapot of the comparative example is improved by 27.4 percent; after 50 times of brewing, the increase rate of the aroma substance concentration of the tea water is obviously reduced to 11.3 percent. Compared with the data of the embodiment 3, the dopamine buffer solution is adopted for soaking treatment, so that the service life of the teapot is obviously prolonged, and the aroma-increasing effect is obviously improved.
While the foregoing is directed to the preferred embodiment of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow.
Claims (10)
1. A preparation method of a selenium-enriched ceramic teapot capable of flavoring tea water is characterized by comprising the following steps:
(1) According to the mass percentage, 75-85% of selenium-rich pottery clay and 15-25% of pore-forming agent are uniformly mixed to obtain a mixture, water accounting for 30-40% of the mass of the mixture is added, ceramic pug is prepared by mixing and ageing, and ceramic pug is adopted to prepare green ceramic teapot blanks;
(2) Drying the prepared green ceramic teapot body, and calcining to obtain a porous ceramic teapot body;
(3) Coating a layer of ceramic glaze on the outer surface of the obtained porous ceramic teapot matrix, then carrying out secondary firing, and cooling along with a furnace to obtain the ceramic teapot;
(4) Filling a ceramic teapot with a dilute acid solution, activating the ceramic surface at a certain temperature, washing and drying after activation;
(5) Filling the ceramic teapot obtained in the step (4) with a cross-linking agent solution, then preserving heat for a certain time at a certain temperature, drying, and filling the dried ceramic teapot with the prepared enzyme solution for adsorption;
(6) And (3) filling a dopamine Tris-HCl buffer solution into the ceramic teapot after adsorption is finished, soaking to strengthen the immobilization effect, washing and drying to obtain the selenium-enriched ceramic teapot capable of enhancing the aroma of tea water.
2. The method for preparing a selenium enriched ceramic teapot capable of flavoring tea as claimed in claim 1, wherein: in the step (1), the content of total selenium in the selenium-rich pottery clay is more than or equal to 0.4mg/kg, the average particle size is 45-75 μm, the pore-forming agent is graphite powder or starch, and the average particle size is 18-25 μm.
3. The method for preparing the selenium-enriched ceramic teapot capable of flavoring tea water as claimed in claim 1, wherein the method comprises the following steps: in the step (2), the firing temperature system of the porous ceramic teapot body is as follows: the heating rate is 5-10 ℃/mi5, the highest temperature point is 1100-1160 ℃, the temperature is respectively kept at 300 ℃, 500 ℃ and 800 ℃ for 15-30 mi5, and the temperature is kept at 90-120 mi5.
4. The method for preparing the selenium-enriched ceramic teapot capable of flavoring tea water as claimed in claim 1, wherein the method comprises the following steps: in the step (3), the ceramic glaze is coated in a glaze dipping mode, and the glaze slip formula for glaze dipping comprises the following components in percentage by mass: 50 to 60 percent of borosilicate glass frit glaze, 40 to 50 percent of water and 0.5 to 1.0 percent of ammonium polyacrylate dispersant.
5. The method for preparing the selenium-enriched ceramic teapot capable of flavoring tea water as claimed in claim 1, wherein the method comprises the following steps: in the step (3), the temperature system of the secondary sintering is as follows: the heating rate is 15-20 ℃/mi5, the highest temperature point is 980-1050 ℃, and the temperature is kept at 20-30 mi5.
6. The method for preparing the selenium-enriched ceramic teapot capable of flavoring tea water as claimed in claim 1, wherein the method comprises the following steps: in the step (4), the ceramic teapot is filled with a dilute hydrochloric acid solution with the mass fraction of 18-25% and then activated for 2-4 hours at the temperature of 85-95 ℃.
7. The method for preparing a selenium enriched ceramic teapot capable of flavoring tea as claimed in claim 1, wherein: in the step (5), a cross-linking agent glutaraldehyde solution with the volume concentration of 1.5-2.0% is filled in the ceramic teapot, and then the ceramic teapot is dried after reacting for 2-4 h at the temperature of 80-90 ℃, wherein the drying temperature is 60-65 ℃.
8. The method for preparing the selenium-enriched ceramic teapot capable of flavoring tea water as claimed in claim 1, wherein the method comprises the following steps: in the step (5), the enzyme solution is prepared by adopting 45-55 mmol/L citric acid-disodium hydrogen phosphate buffer solution to obtain the beta-glucosidase and the beta-primeveroside with the concentration of 200-250U/mL, and the mass ratio of the used enzymes is (1).
9. The method for preparing a selenium enriched ceramic teapot capable of flavoring tea as claimed in claim 1, wherein: in the step (5), the specific operation of adsorption is that the dried teapot is filled with enzyme solution, and the teapot is vacuumized for 1 to 2 hours under the vacuum degree of 85 to 95 kPa; after the vacuum pumping is finished, the adsorption is carried out for 5 to 8 hours at the temperature of between 2 and 5 ℃, and the adsorption process is repeated for 3 to 6 times until the adsorption is finished.
10. The method for preparing the selenium-enriched ceramic teapot capable of flavoring tea water as claimed in claim 1, wherein the method comprises the following steps: in the step (6), a ceramic teapot is filled with dopamine Tris-HCl buffer solution with the concentration of 1.6-2.4 mg/mL and soaked for 12-16 h under the vacuum degree of 85-95 kPa to strengthen the enzyme immobilization effect; finally, washing by adopting 45-55 mmol/L citric acid-disodium hydrogen phosphate buffer solution and drying at 55-65 ℃.
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| CN116396057A (en) * | 2023-03-23 | 2023-07-07 | 四川美术学院 | Selenium-rich ceramic and preparation method thereof |
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