CN115283025B - High-pressure micro-fluidic device - Google Patents
High-pressure micro-fluidic device Download PDFInfo
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- CN115283025B CN115283025B CN202210397167.0A CN202210397167A CN115283025B CN 115283025 B CN115283025 B CN 115283025B CN 202210397167 A CN202210397167 A CN 202210397167A CN 115283025 B CN115283025 B CN 115283025B
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- 238000001816 cooling Methods 0.000 claims description 14
- 230000003993 interaction Effects 0.000 claims description 13
- 238000005070 sampling Methods 0.000 abstract description 3
- 239000012530 fluid Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000005265 energy consumption Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 239000011358 absorbing material Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000005111 flow chemistry technique Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Abstract
The invention relates to the technical field of microfluidics, and discloses a high-pressure microfluidic device, which comprises: the device comprises an air supply device, an air pressure control device, a main control unit 3 and a micro-fluidic chip unit; the air supply device includes: the air compressor is arranged in the first box body and is connected with the first box body through the elastic component 3; the air pressure control device includes: the air conditioner comprises an air storage tank connected with an air compressor through an air pipe, an air pressure sensor arranged on the air storage tank and an air pressure controller arranged between the air storage tank and a micro-fluidic chip unit; an electromagnetic switch is arranged between the air compressor and the air storage tank; the main control unit 3 comprises a main control board 3, and the main control board 3 is electrically connected with the air compressor, the air pressure sensor, the air pressure controller and the electromagnetic switch respectively. The device is provided with the air compressor, does not need an external air source, and is flexible to use. The device is suitable for more microfluidic applications requiring high pressure, can generate positive and negative pressure, can complete automatic sampling and sample introduction of samples, has high degree of automation, and lays a foundation for realizing unmanned systems.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a high-pressure microfluidic device.
Background
The microfluidic technology is to control the fluid under the micron-level size, and micro the experimental functions of biology, chemistry and the like onto a chip to realize the complete experimental function. The sample depends on the fluid flow between different functional modules in the microfluidic chip and the system, so that the driving control of the fluid under the microscale is one of the keys of the microfluidic chip and the system.
Continuous simple media flow control of sample flow in a microfluidic system, i.e., hydrodynamic single-phase flow processing, is the most common form of sample in a microfluidic system. Differential pressure driving is the most widely used driving mode. Differential pressure driving refers to driving fluid flow by utilizing pressure difference to resist viscous force generated by fluid flowing in a micro-channel. The industry usually uses an external air source, an injection pump or a peristaltic pump to generate pressure difference so as to realize fluid driving.
Syringe pumps or peristaltic pumps typically rely on motors to drive, with periodic changes in system pressure control, and reduced stability and accuracy of microfluidic control. The two ways of realizing the system generally contact the processed sample, but in many biochemical experiment occasions, the sample needs to be processed in a non-contact mode, the contact part is realized in a consumable mode, and the processing in a low-cost mode is difficult. Simple air pressure control is relatively stable, but if an external air source is relied on, the use environment condition is inconvenient. And the external air source can not be controlled to generate negative pressure, which is unfavorable for the automation of the whole equipment.
Disclosure of Invention
First, the technical problem to be solved
The invention aims to overcome the defects in the prior art, and provides a high-pressure microfluidic device which can generate high pressure and positive and negative pressure without an external air source and can stably and accurately control the flow rate of a microfluidic.
(II) technical scheme
In order to solve the above problems, the present invention provides a high-pressure microfluidic device comprising: the device comprises an air supply device, an air pressure control device, a main control unit and a micro-fluidic chip unit; the air supply device includes: the air compressor is arranged in the first box body and is connected with the first box body through an elastic component; the air pressure control device includes: the air storage tank is connected with the air compressor through an air pipe, the air pressure sensor is arranged on the air storage tank, and the air pressure controller is arranged between the air storage tank and the micro-fluidic chip unit; an electromagnetic switch is arranged between the air compressor and the air storage tank; the main control unit comprises a main control board, and the main control board is respectively and electrically connected with the air compressor, the air pressure sensor, the air pressure controller and the electromagnetic switch.
Optionally, a sound absorbing layer is disposed on an inner wall of the first box body and is used for absorbing noise generated during operation of the air compressor.
Optionally, a cooling device is arranged in the first box body and is used for cooling the air compressor.
Optionally, a temperature sensor is disposed on the air compressor and is used for detecting the temperature of the air compressor, and the temperature sensor is electrically connected with the main control board.
Optionally, the main control unit further comprises a man-machine interaction module, which is used for displaying the data received by the main control unit and sending out a control instruction.
Optionally, the air supply device further comprises a power supply for supplying power to the air supply device, the air pressure control device and the main control unit.
Optionally, the air supply device, the air pressure control device, the main control unit, the microfluidic chip unit and the power supply are arranged in the outer shell; an openable cover cap is arranged at a position of the outer shell corresponding to the microfluidic chip unit and used for replacing the microfluidic chip unit; and an opening is formed in a position, corresponding to the man-machine interaction module, of the outer shell body, and the opening is used for exposing the man-machine interaction module.
(III) beneficial effects
The high-pressure micro-fluidic device provided by the invention is provided with the air compressor, does not need an external air source, and is flexible to use. The air compressor is fixed through the elastic component and is provided with the sound absorbing layer and the cooling device, and the structural design of the air compressor integrating shock absorption, noise reduction, sound insulation and heat dissipation greatly reduces system noise, so that the noise generated by the air compressor is equivalent to the environmental noise in a laboratory, and the air compressor is suitable for the environmental use in the laboratory. The mode that the air storage tank stores gas with certain pressure to supply air for the micro-fluidic chip unit can reduce the working time and energy consumption of the air compressor and reduce heating. The micro-flow rate of the sample in the micro-fluidic chip unit can be stably and accurately controlled by the air pressure control device. The sample is processed in a non-contact mode, so that the sample processing is finished in the disposable consumable material with low cost, and the large-scale low-cost business can be realized. The self-contained air compressor and electromagnetic switch are applicable to more micro-fluidic applications requiring high pressure, positive and negative pressure can be generated, positive and negative pressure switching can be realized through the main control unit, automatic sampling and sample injection of samples are completed, the automation degree is high, and a foundation is laid for unmanned system realization.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is evident that the drawings in the following description are only some embodiments of the present invention and that other drawings may be obtained from these drawings without inventive effort for a person of ordinary skill in the art.
Fig. 1 is a schematic diagram of an internal structure of a high-pressure microfluidic device according to an embodiment of the present invention;
Fig. 2A is a schematic diagram of an external structure of a gas supply device of a high-pressure microfluidic device according to an embodiment of the present invention;
fig. 2B is a schematic diagram of an internal structure of a gas supply device of the high-pressure micro-fluidic device according to an embodiment of the present invention;
fig. 3 is a schematic diagram of an external structure of a high-pressure microfluidic device according to an embodiment of the present invention.
The reference numerals in the drawings are in turn:
1. the device comprises an air supply device 11, a first box body 12, an air compressor 13, an elastic component 14, an elastic component support 2, an air pressure control device 21, an air storage tank 22, an air pressure controller 3, a main control unit 31, a main control board 32, a man-machine interaction module 4, a micro-fluidic chip unit 5, a cooling device 6, a power supply 7, an outer shell 71, a cover 72 and a base.
Detailed Description
The following describes the embodiments of the present invention in further detail with reference to examples and drawings. The following examples of the present invention are presented herein for the purpose of illustration and are not intended to limit the scope of the invention.
As shown in fig. 1, an embodiment of the present invention provides a high-pressure microfluidic device, including: the device comprises an air supply device 1, an air pressure control device 2, a main control unit 3 and a micro-fluidic chip unit 4. Wherein,
The air supply device 1 includes: the air compressor 12 is disposed in the first casing 11, and the air compressor 12 is connected to the first casing 11 through an elastic member 13, as shown in fig. 2A and 2B. The elastic assembly 13 includes a plurality of elastic members, such as damper springs, connected between the air compressor 12 and the frame of the first casing 11. As shown in fig. 3, a plurality of elastic member holders 14 are provided on the air compressor 12, for example, elastic member holders 14 are provided at four corners of the bottom of the air compressor 12 and at the middle of the top surface of the air compressor 12 for fixing one end of each elastic member in the elastic assembly 13, and fixing points capable of fixing the other end of the elastic member are provided at corresponding positions on the frame of the first casing 11. Two ends of each elastic component in the elastic component 13 are respectively fixed on an elastic component bracket 14 and a fixed point corresponding to the position of the elastic component bracket, so that the air compressor 12 and the frame of the first box 11 are fixed together through a plurality of elastic components; the air compressors 12 are arranged side by side in a suspended manner in the first housing 11. In this way, when the air compressor 12 is severely vibrated in the operating state, the plurality of elastic members for fixing the air compressor 12 can greatly reduce vibration noise. The tension of each elastic component can be adjusted according to the requirement, so that the air compressor 12 is stressed uniformly, and the balanced state is maintained.
In order to reduce noise generated when the air compressor 12 is operated, a sound absorbing layer (not shown) may be further provided on the inner wall of the first casing 11 for absorbing noise generated when the air compressor is operated. For example, a sound absorbing material such as sound absorbing cotton, a sound absorbing board, or the like is applied to the inner wall of the first casing 11. Thereby further reducing noise generated when the air compressor 12 is operated.
The air pressure control device 2 includes: an air tank 21 connected to the air compressor 12 through an air pipe (not shown), an air pressure sensor (not shown) provided on the air tank 21, and an air pressure controller 22 provided between the air tank 21 and the microfluidic chip unit 4. An electromagnetic switch (not shown) is provided between the air compressor 12 and the air tank 21; the main control unit 3 includes a main control board 31, and the main control board 31 is electrically connected to the air compressor 12, an air pressure sensor (not shown), the air pressure controller 22, and the electromagnetic switch, respectively.
The air compressor 12 inflates the air storage tank 21 through an air pipe, and simultaneously the air pressure sensor detects the air pressure in the air storage tank 21 in real time and sends the detected air pressure value to the main control unit 3. When the main control unit 3 detects that the air pressure value in the air storage tank 21 reaches the set air pressure value, the main control board 31 in the main control unit 3 sends an instruction to the air compressor 12 to stop the air compressor 12. Then, the main control board 3 sends an instruction to the air pressure controller 22 so that the air pressure controller 22 supplies air to the microfluidic chip unit 4 at an air pressure of a set air pressure value (at an air pressure of a certain air pressure value lower than the air pressure value in the air reservoir 21) so that the liquid sample to be detected in the microfluidic chip unit 4 enters the microfluidic chip in the microfluidic chip unit 4. The main control board 31 controls the air pressure value of the air supplied to the micro-fluidic chip unit 4 through the air pressure controller 22, thereby realizing control of the flow rate of the liquid in the micro-fluidic chip unit 4. The microfluidic chip unit 4 is in the form of a consumable pack detachably connected to the air pressure controller 22. By means of the control mode, air supply to the air storage tank 21 can be intelligently controlled, and air source working time and energy consumption are reduced. The micro flow rate of the sample in the micro flow control chip unit 4 can also be controlled stably and precisely.
When a sample to be processed needs to be extracted from the microfluidic chip unit 4, the main control board 3 sends an instruction to the air compressor 12, controls the air compressor 12 to work to generate negative pressure, and switches the corresponding electromagnetic switch, so that the sample is automatically extracted into the microfluidic chip unit 4.
Since the first casing 11 is a closed structure and the sound absorbing layer is provided on the inner wall, the heat generated when the air compressor 12 in the first casing 11 works causes the temperature in the first casing 11 to rise. For this purpose, as shown in fig. 1 to 3, the cooling device 5 is provided in the first casing 11, and for example, a liquid cooling heat transfer sheet is provided at the top of the air compressor 12, and heat is transferred out of the first casing 11 by a liquid cooling system. A fan may be provided outside the first casing 11 to radiate heat from the first casing 11.
In order to timely radiate heat of the first box 11, a temperature sensor (not shown in the figure) may be disposed on the air compressor 12, and used for detecting the temperature of the air compressor 12 in real time, where the temperature sensor is electrically connected with the main control unit 3, and sending the detected temperature data of the air compressor 12 to the main control unit 3. When the temperature of the air compressor 12 exceeds the set temperature, the main control unit 3 sends an instruction to the cooling device 5, and the cooling device 5 is started to cool and dissipate heat of the air compressor 12. When the temperature of the air compressor 12 falls below the set temperature, the main control unit 3 sends an instruction to the cooling device 5. The cooling device 5 is stopped.
The main control unit 3 further comprises a man-machine interaction module 32, which is used for displaying the data received by the main control unit 3 and sending out control instructions. The man-machine interaction module 32 is electrically connected with the main control board 31, and is provided with a man-machine interaction module for displaying various data received by the main control board 31, for example, the air pressure value in the air storage tank 21 detected by the air pressure sensor arranged on the air storage tank 21; a temperature value detected by a temperature sensor provided in the air compressor 12. The main control board 31 can be controlled to send various instructions through the man-machine interaction module.
A power supply 6 is also arranged for supplying power to the components in the air supply device 1, the air pressure control device 2 and the main control unit 3.
As shown in fig. 1 and 3, the air supply device 1, the air pressure control device 2, the main control unit 3, the microfluidic chip unit 4 and the power supply 6 are arranged in an outer shell 7; wherein the air supply device 1, the air pressure control device 2 and the main control unit 3 are all fixed on the base 72 of the outer shell 7. The part of the outer shell 7 corresponding to the microfluidic chip unit 4 is provided with an openable cover 71 for replacing the microfluidic chip unit 4; the part of the outer shell 7 corresponding to the man-machine interaction module 32 is provided with an opening for exposing the man-machine interaction module 32.
The high-pressure micro-fluidic device provided by the invention is provided with the air compressor, does not need an external air source, and is flexible to use. The air compressor is fixed through the elastic component and is provided with the sound absorbing layer and the cooling device, and the structural design of the air compressor integrating shock absorption, noise reduction, sound insulation and heat dissipation greatly reduces system noise, so that the noise generated by the air compressor is equivalent to the environmental noise in a laboratory, and the air compressor is suitable for the environmental use in the laboratory. The mode that the air storage tank stores gas with certain pressure to supply air for the micro-fluidic chip unit can reduce the working time and energy consumption of the air compressor and reduce heating. The micro-flow rate of the sample in the micro-fluidic chip unit can be stably and accurately controlled by the air pressure control device. The sample is processed in a non-contact mode, so that the sample processing is finished in the disposable consumable material with low cost, and the large-scale low-cost business can be realized. The self-contained air compressor and electromagnetic switch are applicable to more micro-fluidic applications requiring high pressure, positive and negative pressure can be generated, positive and negative pressure switching can be realized through the main control unit, automatic sampling and sample injection of samples are completed, the automation degree is high, and a foundation is laid for unmanned system realization.
In the present invention, terms such as "upper", "lower", "left", "right", "front", "rear", "vertical", "horizontal", "side", "bottom", etc. refer to an orientation or a positional relationship based on that shown in the drawings, and are merely relational terms, which are used for convenience in describing structural relationships of various components or elements of the present invention, and do not denote any one of the components or elements of the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. It will be understood by those of ordinary skill in the art that the specific meaning of the terms above in the present invention is not to be construed as limiting the invention.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
The above embodiments are only for illustrating the present invention, and are not limiting of the present invention. While the invention has been described in detail with reference to the embodiments, those skilled in the art will appreciate that various combinations, modifications, and substitutions can be made thereto without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (7)
1. A high-pressure microfluidic device, comprising: the device comprises an air supply device (1), an air pressure control device (2), a main control unit (3) and a micro-fluidic chip unit (4);
The air supply device (1) comprises: an air compressor (12) arranged in a first box body (11), wherein the air compressor (12) is connected with the first box body (11) through an elastic component (13), the elastic component (13) comprises a plurality of elastic components connected between the air compressor (12) and a frame of the first box body (11), elastic component brackets (14) are arranged at the four corners of the bottom of the air compressor (12) and the middle of the top surface of the air compressor (12) and used for fixing one end of each elastic component in the elastic component (13), fixed points capable of fixing the other end of the elastic component are arranged at corresponding positions on the frame of the first box body (11) so as to fix two ends of each elastic component in the elastic component brackets (14) and the fixed points corresponding to the positions of the elastic components respectively, and the air compressor (12) is arranged in the first box body (11) in a suspending manner;
The air pressure control device (2) comprises: the air compressor comprises an air storage tank (21) connected with the air compressor (12) through an air pipe, an air pressure sensor arranged on the air storage tank (21), and an air pressure controller (22) arranged between the air storage tank (21) and the micro-fluidic chip unit (4);
An electromagnetic switch is arranged between the air compressor (12) and the air storage tank (21);
The main control unit (3) comprises a main control board (31), and the main control board (31) is electrically connected with the air compressor (12), the air pressure sensor, the air pressure controller (22) and the electromagnetic switch respectively.
2. The high-pressure microfluidic device according to claim 1, wherein a sound absorbing layer is provided on an inner wall of the first housing (11) for absorbing noise generated when the air compressor (12) operates.
3. The high-pressure microfluidic device according to claim 2, wherein a cooling device (5) is arranged in the first box (11) for cooling the air compressor (12).
4. The high-pressure microfluidic device according to claim 2, wherein a temperature sensor is arranged on the air compressor (12) and is used for detecting the temperature of the air compressor (12), and the temperature sensor is electrically connected with the main control board (31).
5. The high-pressure micro-fluidic device according to claim 1, wherein the main control unit (3) further comprises a man-machine interaction module (32) for displaying data received by the main control unit (3) and sending out control instructions.
6. The high-pressure microfluidic device according to claim 5, further comprising a power supply (6) for powering the gas supply device (1), the gas pressure control device (2) and the master control unit (3).
7. The high-pressure microfluidic device of claim 6, wherein,
The air supply device (1), the air pressure control device (2), the main control unit (3), the micro-fluidic chip unit (4) and the power supply (6) are arranged in the outer shell (7);
The part of the outer shell (7) corresponding to the microfluidic chip unit (4) is provided with an openable cover cap (71) for replacing the microfluidic chip unit (4);
And an opening is formed in a part of the outer shell (7) corresponding to the man-machine interaction module (32) and used for exposing the man-machine interaction module (32).
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210397167.0A CN115283025B (en) | 2022-04-15 | High-pressure micro-fluidic device | |
| PCT/CN2022/123306 WO2023197538A1 (en) | 2022-04-15 | 2022-09-30 | High-gas pressure microfluidic device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210397167.0A CN115283025B (en) | 2022-04-15 | High-pressure micro-fluidic device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115283025A CN115283025A (en) | 2022-11-04 |
| CN115283025B true CN115283025B (en) | 2024-07-05 |
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|---|---|---|---|---|
| CN205447273U (en) * | 2016-02-16 | 2016-08-10 | 苏州汶颢芯片科技有限公司 | Multichannel fluid controlling means |
| CN206133401U (en) * | 2016-08-29 | 2017-04-26 | 重庆明宇科技有限公司 | Hard disk of computer shock attenuation installation protection device |
| CN110594592A (en) * | 2019-10-24 | 2019-12-20 | 衢州职业技术学院 | Energy-saving multi-air-compressor air supply system |
| CN111823459A (en) * | 2020-06-23 | 2020-10-27 | 江苏敦超电子科技有限公司 | PDMS pouring system and pouring method for manufacturing micro-fluidic chip |
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205447273U (en) * | 2016-02-16 | 2016-08-10 | 苏州汶颢芯片科技有限公司 | Multichannel fluid controlling means |
| CN206133401U (en) * | 2016-08-29 | 2017-04-26 | 重庆明宇科技有限公司 | Hard disk of computer shock attenuation installation protection device |
| CN110594592A (en) * | 2019-10-24 | 2019-12-20 | 衢州职业技术学院 | Energy-saving multi-air-compressor air supply system |
| CN111823459A (en) * | 2020-06-23 | 2020-10-27 | 江苏敦超电子科技有限公司 | PDMS pouring system and pouring method for manufacturing micro-fluidic chip |
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