CN111823459A - PDMS pouring system and pouring method for manufacturing micro-fluidic chip - Google Patents
PDMS pouring system and pouring method for manufacturing micro-fluidic chip Download PDFInfo
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- CN111823459A CN111823459A CN202010578102.7A CN202010578102A CN111823459A CN 111823459 A CN111823459 A CN 111823459A CN 202010578102 A CN202010578102 A CN 202010578102A CN 111823459 A CN111823459 A CN 111823459A
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- 239000004205 dimethyl polysiloxane Substances 0.000 title claims abstract description 185
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 title claims abstract description 185
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 16
- 235000013870 dimethyl polysiloxane Nutrition 0.000 title claims 42
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 title claims 42
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 title claims 42
- 238000002156 mixing Methods 0.000 claims abstract description 68
- 238000003860 storage Methods 0.000 claims abstract description 58
- 238000001125 extrusion Methods 0.000 claims abstract description 50
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 40
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C39/00—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor
- B29C39/02—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor for making articles of definite length, i.e. discrete articles
- B29C39/10—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor for making articles of definite length, i.e. discrete articles incorporating preformed parts or layers, e.g. casting around inserts or for coating articles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C39/00—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor
- B29C39/22—Component parts, details or accessories; Auxiliary operations
- B29C39/24—Feeding the material into the mould
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C39/00—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor
- B29C39/22—Component parts, details or accessories; Auxiliary operations
- B29C39/44—Measuring, controlling or regulating
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B81—MICROSTRUCTURAL TECHNOLOGY
- B81C—PROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS
- B81C1/00—Manufacture or treatment of devices or systems in or on a substrate
- B81C1/00015—Manufacture or treatment of devices or systems in or on a substrate for manufacturing microsystems
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- Engineering & Computer Science (AREA)
- Manufacturing & Machinery (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Casting Or Compression Moulding Of Plastics Or The Like (AREA)
Abstract
The invention relates to a PDMS (polydimethylsiloxane) pouring system and a pouring method for manufacturing a microfluidic chip, which comprises a PDMS pouring control unit, wherein the PDMS pouring control unit comprises a computer, a pressure controller, an air compressor and a vacuum pump, wherein the air compressor and the vacuum pump are connected with the pressure controller; the PDMS pouring and batching unit comprises a storage tank and a mixing barrel, wherein the output end of a pressure controller is respectively connected with an air inlet of the storage tank and an air inlet of the mixing barrel, a discharge port of the storage tank is connected with the mixing barrel, and a storage tank flowmeter is arranged between the storage tank and the mixing barrel; the PDMS casting molding unit comprises an extrusion device, a chip mold and a three-dimensional motion platform in communication connection with a computer. The invention automatically realizes the feeding proportion of the PDMS prepolymer and the PDMS curing agent, and has high precision; meanwhile, the integrated design of PDMS pouring is realized through a computer program, the whole PDMS pouring process is automatically controlled, manual operation is not needed, and the labor cost is reduced.
Description
Technical Field
The invention relates to the field of micro-fluidic chip manufacturing, in particular to a PDMS (polydimethylsiloxane) pouring system and a pouring method for manufacturing micro-fluidic chips.
Background
The micro-fluidic chip integrates micro-components with different functions, such as a micro-channel, a micro-pump, a micro-valve, a micro-liquid storage device, a micro-electrode, a micro-detection element, a connector and the like, by a micro-processing technology, so that the micro-component becomes a micro total analysis system. The microfluidic technology integrates the experimental processes of sampling, preprocessing, separation, characterization and the like required by biological sample detection on a chip with a micron-sized channel network, and has the advantages of low sample consumption, high control precision, sensitive detection, short analysis time and the like. With the development of economic society, the application of the microfluidic technology in medical diagnosis and the like is more and more extensive, and the microfluidic technology has obvious advantages in the aspects of detection precision, detection speed and the like.
The function and structure of the micro-fluidic chip are realized by various advanced micro-fluidic chip processing technologies, the micro-electromechanical system is originated from the micro-fluidic chip processing method, and the micro-scale pattern transfer is realized by utilizing the photoetching and etching technologies of the semiconductor processing technology. Originally, the main material used for microfluidic chip processing was glass, but the isotropic characteristics of glass made the processing of high aspect ratio channels difficult to achieve, and the channels of glass material were difficult to achieve sealing. The conventional microfluidic processing methods include stamping, injection molding and the like, but the processing methods are high in price, complex in processing procedure and large in occupied manpower and material resources. The soft lithography technology developed based on the lithography technology is the most common processing method in the microfluidic field at present, and has the advantages of high processing precision, good biocompatibility, good light transmittance and the like. However, the PDMS casting process involved in the soft lithography technology is complex and has low automation degree, which severely limits the popularization and application of the microfluidic chip and the industrial mass production. Therefore, a system with high automation degree, simple operation and accurate quantification is needed for pouring the microfluidic chip.
Disclosure of Invention
The invention provides a PDMS casting system and a casting method for manufacturing a microfluidic chip, aiming at the defects of the existing manufacturing method for the microfluidic chip.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a PDMS gating system for manufacturing micro-fluidic chips comprises
The PDMS pouring control unit comprises a computer, a pressure controller, an air compressor connected with the positive pressure input end of the pressure controller and a vacuum pump connected with the negative pressure input end of the pressure controller, wherein the computer is connected with the pressure controller and is used for adjusting the output state of the pressure controller;
the PDMS pouring and batching unit comprises a storage tank and a mixing barrel, wherein the output end of a pressure controller is respectively connected with an air inlet of the storage tank, the discharge port of the storage tank is connected with the mixing barrel, a storage tank flowmeter is arranged between the storage tank and the mixing barrel, and the storage tank flowmeter is connected with a computer and used for setting and monitoring the discharge amount of the storage tank;
the PDMS casting molding unit comprises an extrusion device, a chip mold and a three-dimensional moving platform in communication connection with a computer, wherein an extrusion nozzle of the extrusion device is fixed on the three-dimensional moving platform, and the chip mold is located right below the extrusion nozzle.
Further, PDMS pouring batching unit includes PDMS mixing stirring module, PDMS mixing stirring module include stirring rake pneumatic motor and with driving gear, driven gear, hollow shaft and the stirring rake that stirring rake pneumatic motor links to each other in proper order, driving gear and driven gear meshing transmission, driven gear installs the top at the hollow shaft, and the stirring rake is installed in the bottom of hollow shaft, and stirring rake pneumatic motor connects the pressure controller who is used for exerting pressure for stirring rake pneumatic motor.
Further, extrusion device is including extruding shower nozzle, case cylinder and case, and the case passes hollow shaft one end and links to each other with the case cylinder, and the discharge gate of compounding bucket is connected to the other end, and the case cylinder links to each other with pressure controller for the lid of control case and discharge gate closes the state, extrude the discharge gate tube coupling of shower nozzle and compounding bucket, and be equipped with the compounding bucket on the pipeline and extrude the flowmeter, the compounding bucket is extruded the flowmeter and is linked to each other with the computer for the discharge capacity of setting and monitoring compounding bucket.
Further, the storing tank includes PDMS prepolymer storage tank and PDMS curing agent storage tank, and the storage tank flowmeter includes PDMS prepolymer flowmeter and PDMS curing agent flowmeter, links to each other with the computer respectively for the load of setting and monitoring PDMS prepolymer and PDMS curing agent.
Further, the output end of the pressure controller is connected with the mixing barrel and used for applying negative pressure to the mixing barrel to eliminate bubbles of PDMS mixed liquid in the mixing barrel.
Furthermore, the pressure controller is provided with a plurality of positive pressure output ends and a plurality of negative pressure output ends, and a software program for controlling the output state of the pressure controller is arranged in the computer.
A PDMS casting method for manufacturing a microfluidic chip comprises the following steps:
preparing raw materials, filling a PDMS prepolymer and a PDMS curing agent, setting a positive pressure value of a pressure controller, and applying positive pressure to a storage tank to realize that the PDMS prepolymer and the PDMS curing agent are respectively conveyed to a mixing barrel;
stirring the PDMS mixed liquid, applying positive pressure to a stirring paddle pneumatic motor by a pressure controller, and starting the stirring paddle pneumatic motor so as to drive the stirring paddle to rotate and realize stirring of the PDMS mixed liquid in the mixing barrel; when the PDMS mixed liquid in the mixing barrel is uniformly stirred, the pressure transmission channel is closed, and the stirring paddle stops stirring;
defoaming the PDMS mixed solution, applying negative pressure to the mixing barrel by using a pressure controller, performing bubble removal treatment on the PDMS mixed solution, and closing a pressure conveying channel after the PDMS mixed solution is defoamed;
extruding the PDMS mixed liquid, applying positive pressure to a valve core cylinder by a pressure controller, lifting the valve core to expose a discharge hole positioned at the bottom of a mixing barrel, and applying positive pressure to the mixing barrel by the pressure controller to extrude the PDMS mixed liquid from an extrusion nozzle;
and (3) PDMS pouring, wherein the three-dimensional motion platform is controlled by the computer to enable the extrusion nozzle to move above the chip die, so that the precise pouring of the PDMS mixed liquid is realized.
Further, when the discharge amount of the storage tank reaches a set value, the flow meter of the storage tank feeds back a turn-off signal to the computer, and the computer controls the pressure controller to close the positive pressure channel, so that feeding of the PDMS prepolymer and the PDMS curing agent is stopped.
Further, when PDMS is poured and the extrusion amount reaches a set value, the mixing barrel extrusion flowmeter feeds back a turn-off signal to the computer, and the computer controls the pressure controller to close the positive pressure channel, so that extrusion of PDMS mixed liquid is stopped.
Compared with the prior art, the invention has the advantages that;
the invention automatically realizes the feeding proportion of the PDMS prepolymer and the PDMS curing agent, and has high precision;
the invention realizes the integrated design of PDMS casting through a computer program, automatically controls the whole PDMS casting process, does not need manual operation and reduces the labor cost.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is apparent that the drawings in the following description are only some embodiments of the present invention. Wherein:
FIG. 1 is a schematic structural diagram of a PDMS gating system for manufacturing microfluidic chips according to an embodiment of the present invention;
FIG. 2 is a flow chart of a PDMS casting method for microfluidic chip fabrication according to an embodiment of the present invention;
in the figure: 1. vacuum pump, 2, air compressor, 3, pressure controller, 4, PDMS prepolymer storage tank, 5, PDMS curing agent storage tank, 6, PDMS prepolymer flowmeter, 7, PDMS curing agent flowmeter, 8, stirring rake pneumatic motor, 9, case cylinder, 10, driving gear, 11, driven gear, 12, compounding bucket, 13, case, 14, hollow shaft, 15, stirring rake, 16, extrude the shower nozzle, 17, compounding bucket extrude the flowmeter, 18, three-dimensional motion platform, 19, chip mould, 20, the computer.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, not all, embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, shall fall within the scope of protection of the present invention.
Fig. 1 shows a schematic structural diagram of a PDMS casting system for manufacturing a microfluidic chip according to the present invention, which is composed of a PDMS casting control unit, a PDMS casting batching unit, and a PDMS casting molding unit.
PDMS pours the control unit, includes computer 20, pressure controller 3, the air compressor 2 who links to each other with pressure controller 3 positive pressure input end, the vacuum pump 1 who links to each other with pressure controller 3 negative pressure input end, computer 20 links to each other with pressure controller 3 for the output state of adjustment pressure controller 3.
The pressure controller 3 comprises a negative pressure input end, a positive pressure input end and five positive and negative pressure output ends, the positive pressure input end of the pressure controller 3 is connected with an air compressor 2, the negative pressure input end of the pressure controller 3 is connected with a vacuum pump 1, the output end A and the output end B of the pressure controller 3 are respectively connected with a PDMS prepolymer storage tank 4 and a PDMS curing agent storage tank 5, the output end C of the pressure controller 3 is connected with a mixing barrel 12, the output end D of the pressure controller 3 is connected with a stirring paddle pneumatic motor 8, and the output end E of the pressure controller 3 is connected with a valve core cylinder 9.
The PDMS pouring and batching unit comprises a storage tank and a mixing barrel 12, wherein the output end of a pressure controller 3 is respectively connected with an air inlet of the storage tank, a discharge port of the storage tank is connected with the mixing barrel, and a storage tank flowmeter is arranged on a pipeline between the storage tank and the mixing barrel; wherein, the storing tank includes PDMS prepolymer storage tank 4 and PDMS curing agent storage tank 5, and the storage tank flowmeter includes PDMS prepolymer flowmeter 6 and PDMS curing agent flowmeter 7, links to each other with computer 20 respectively for the load of setting and monitoring PDMS prepolymer and PDMS curing agent.
The computer 20 controls the output end A of the pressure controller 3 to apply positive pressure to the PDMS prepolymer storage tank 4, so that the PDMS prepolymer is fed. The PDMS prepolymer flowmeter 6 monitors the discharge amount of the PDMS prepolymer storage tank 4, when the discharge amount reaches a set value, the PDMS prepolymer flowmeter 6 feeds back a turn-off signal to the computer 20, and the computer 20 controls the pressure controller 3 to close the air pressure channel, so that feeding of the PDMS prepolymer is stopped.
The computer 20 controls the output end B of the pressure controller 3 to apply positive pressure to the PDMS curing agent storage tank 5, so that the feeding of the PDMS curing agent is realized. The PDMS curing agent flowmeter 7 monitors the discharge amount of the PDMS curing agent storage tank 5, when the discharge amount reaches a set value, the PDMS curing agent flowmeter 7 feeds back a turn-off signal to the computer 20, and the computer 20 controls the pressure controller 3 to close the air pressure channel, so that the feeding of the PDMS curing agent is stopped.
PDMS mixes stirring module, including stirring rake pneumatic motor 8 and the driving gear 10, driven gear 11, hollow shaft 14 and the stirring rake 15 that link to each other in proper order with stirring rake pneumatic motor 8, driving gear 10 and driven gear 11 meshing transmission, driven gear 11 is installed on the top of hollow shaft 14, and stirring rake 15 is installed in the bottom of hollow shaft 14, and stirring rake pneumatic motor 8 connection pressure control 3 is used for exerting pressure for stirring rake pneumatic motor 8.
The computer 20 controls the output end D of the pressure controller 3 to apply positive pressure to the pneumatic motor 8 of the stirring paddle, so that the pneumatic motor 8 of the stirring paddle rotates, and drives the stirring paddle 15 to rotate sequentially through the driving gear 10, the driven gear 11 and the hollow shaft 14, so that the PDMS mixed liquid in the mixing barrel 12 is stirred.
The PDMS casting molding unit comprises an extrusion device, a chip mold 19 and a three-dimensional motion platform 18 which is in communication connection with a computer 20, wherein an extrusion nozzle 16 of the extrusion device is installed on the three-dimensional motion platform 18, and the chip mold 19 is located right below the extrusion nozzle 16.
The extrusion device comprises an extrusion nozzle 16, a valve core cylinder 9 and a valve core 13, wherein the valve core 13 penetrates through one end of a hollow shaft 14 to be connected with the valve core cylinder 9, the other end of the hollow shaft is connected with a discharge hole of a mixing barrel 12, the valve core cylinder 9 is connected with a pressure controller 3 and used for controlling the covering state of the valve core 13 and the discharge hole, the extrusion nozzle 16 is connected with a discharge hole pipeline of the mixing barrel 12, a mixing barrel extrusion flow meter 17 is arranged on the pipeline, and the mixing barrel extrusion flow meter 17 is connected with a computer 20 and used for setting and monitoring the discharge amount of the mixing barrel 12.
The computer 20 controls the output end C of the pressure controller 3 to apply negative pressure to the mixing barrel 12, so as to remove bubbles from the PDMS mixed liquid.
The computer 20 controls the output end E of the pressure controller 3 to apply positive pressure to the valve core cylinder 9, and the valve core 13 is lifted to expose the discharge hole at the bottom of the mixing barrel 12. Subsequently, the computer 20 controls the output end C of the pressure controller 3 to apply positive pressure to the mixing barrel 12, so that the PDMS mixed liquid is extruded from the extrusion nozzle 16.
The computer 20 controls the three-dimensional moving platform 18 to enable the extrusion nozzle 16 to move above the chip die 19, so that the PDMS mixed liquid is accurately poured. The mixing barrel extrusion flowmeter 17 monitors the extrusion amount of the PDMS mixed liquid, when the extrusion amount reaches a set value, namely the chip pouring is completed, the mixing barrel extrusion flowmeter 17 feeds back a turn-off signal to the computer 20, and the computer 20 controls the pressure controller 3 to close the positive pressure channel, so that the extrusion of the PDMS mixed liquid is stopped.
Fig. 2 shows a flow chart of the PDMS casting method for microfluidic chip fabrication of the present invention, comprising the following steps:
step one; preparing raw materials, respectively adding a certain amount of filled PDMS prepolymer and PDMS curing agent into a PDMS prepolymer storage tank 4 and a PDMS curing agent storage tank 5, and sealing well;
starting the air compressor 2 and the vacuum pump 1, setting the output end A and the output end B of the pressure controller 3 to be under positive pressure action through the computer 20, and conveying the PDMS prepolymer and the PDMS curing agent to the mixing barrel 12;
when the discharge amount reaches a set value, the PDMS prepolymer flowmeter 6 and the PDMS curing agent flowmeter 7 feed back a turn-off signal to the computer 20, and the computer 20 closes the air pressure channels of the output end a and the output end B of the pressure controller 3, thereby stopping feeding of the PDMS prepolymer and the curing agent.
Step two; stirring the PDMS mixed liquid, wherein the computer 20 controls the output end D of the pressure controller 3 to apply positive pressure to the pneumatic motor 8 of the stirring paddle, and the pneumatic motor 8 of the stirring paddle rotates to drive the stirring paddle 15 to rotate so as to realize stirring of the PDMS mixed liquid in the mixing barrel 12;
after the PDMS mixed liquid is uniformly stirred, the computer 20 closes the pressure transmission channel at the output end D of the pressure controller 3, and the stirring paddle 15 stops stirring the PDMS mixed liquid.
Step three; removing bubbles from the PDMS mixed solution, wherein the computer 20 controls the output end C of the pressure controller 3 to apply a negative pressure effect to the mixing barrel 12 to remove the bubbles from the PDMS mixed solution; after a period of time, the computer 20 closes the air pressure channel at the output end C of the pressure controller 3, stops applying the negative pressure to the mixing barrel 12, and the defoaming of the PDMS mixed solution is completed.
Extruding the PDMS mixed liquid, controlling an output end E of the pressure controller 3 by the computer 20 to apply positive pressure to the valve core cylinder 9, lifting the valve core 13 to expose the discharge hole at the bottom of the mixing barrel 12, and applying positive pressure to the mixing barrel 12 by an output end C of the pressure controller 3 to extrude the PDMS mixed liquid from the extrusion nozzle.
And (3) PDMS pouring, wherein the computer 20 controls the three-dimensional motion platform 18 to enable the extrusion nozzle 16 to move above the chip mold 19, so that the precise pouring of the PDMS mixed liquid is realized.
When the extrusion amount reaches a set value, the material mixing barrel extrusion flowmeter 17 feeds a turn-off signal back to the computer 20, and the computer 20 closes the positive pressure channel at the output end C of the pressure controller 3, so that extrusion of the PDMS mixed liquid is stopped.
As an embodiment of the present invention, the following specific examples are used to illustrate the operation flow of the inventive PDMS casting method for microfluidic chip fabrication:
s1, taking 300g of PDMS prepolymer and putting the PDMS prepolymer into a PDMS prepolymer storage tank, taking 200g of PDMS curing agent and putting the PDMS curing agent into a PDMS curing agent storage tank, and sealing the two storage tanks well.
S2, starting the air compressor and the vacuum pump, and setting the pressure of the air compressor and the pressure of the vacuum pump to reach 0.1MPa and-0.1 MPa respectively;
s3, setting an output end A and an output end B of a pressure controller to be under positive pressure, respectively conveying the PDMS prepolymer in the storage tank A and the curing agent in the storage tank B to a mixing barrel under the action of air pressure, wherein the raw material ratio of the PDMS mixed liquid for PDMS chip pouring is 10: 1;
s4, monitoring the discharge amount of the PDMS prepolymer storage tank by the PDMS prepolymer flow meter, feeding a turn-off signal back to the computer by the PDMS prepolymer flow meter when the PDMS prepolymer in the storage tank A reaches 200g, and turning off the air pressure channel of the output end A by the computer by the pressure controller so as to stop feeding of the PDMS prepolymer;
s5, monitoring the discharge amount of the PDMS curing agent storage tank by the PDMS curing agent flow meter, feeding a turn-off signal to the computer by the PDMS curing agent flow meter when the PDMS curing agent reaches 20g, and turning off the air pressure channel of the output end B by the pressure controller by the computer so as to stop feeding of the PDMS curing agent;
s6, the output end D of the computer control pressure controller applies positive pressure to the pneumatic motor of the stirring paddle to realize the rotation of the pneumatic motor of the stirring paddle, and drives the stirring paddle to rotate sequentially through the driving gear and the driven gear to realize the stirring of the PDMS mixed liquid in the mixing barrel;
s7, after stirring the PDMS mixed liquid in the mixing barrel uniformly for about 10 minutes, closing the pressure transmission channel at the output end D of the pressure controller by the computer, stopping the rotation of the pneumatic motor of the stirring paddle, and stopping the stirring of the PDMS mixed liquid by the stirring paddle;
s8, the output end C of the computer control pressure controller applies negative pressure to the mixing barrel to remove bubbles from the PDMS mixed liquid, and the process of removing bubbles from the PDMS mixed liquid lasts for about 5 minutes;
s9, after defoaming the PDMS mixed solution, the output end C of the computer controls the pressure controller to apply positive pressure to the valve core cylinder, lift the valve core and expose the discharge hole at the bottom of the mixing barrel;
s10, subsequently, the output end C of the computer-controlled pressure controller applies positive pressure to the mixing barrel to extrude the PDMS mixed liquid from the extrusion nozzle;
and S11, controlling the three-dimensional motion platform by the computer to enable the extrusion nozzle to move above the chip die according to a set route, and realizing accurate pouring of the PDMS mixed liquid.
When the chip pouring is completed, the mixing barrel extrusion flowmeter feeds a turn-off signal back to the computer, and the computer controls the pressure controller to close the positive pressure channel at the output end, so that the extrusion of the PDMS mixed liquid is stopped.
While the invention has been described with reference to specific embodiments, the invention is not limited thereto, and various equivalent modifications and substitutions can be easily made by those skilled in the art within the technical scope of the invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (9)
1. A PDMS gating system for manufacturing a microfluidic chip is characterized in that: comprises that
The PDMS pouring control unit comprises a computer, a pressure controller, an air compressor connected with the positive pressure input end of the pressure controller and a vacuum pump connected with the negative pressure input end of the pressure controller, wherein the computer is connected with the pressure controller and is used for adjusting the output state of the pressure controller;
the PDMS pouring and batching unit comprises a storage tank and a mixing barrel, wherein the output end of a pressure controller is respectively connected with an air inlet of the storage tank, the discharge port of the storage tank is connected with the mixing barrel, a storage tank flowmeter is arranged between the storage tank and the mixing barrel, and the storage tank flowmeter is connected with a computer and used for setting and monitoring the discharge amount of the storage tank;
the PDMS casting molding unit comprises an extrusion device, a chip mold and a three-dimensional moving platform in communication connection with a computer, wherein an extrusion nozzle of the extrusion device is fixed on the three-dimensional moving platform, and the chip mold is located right below the extrusion nozzle.
2. A PDMS runner system according to claim 1, characterised in that: the PDMS pouring batching unit comprises a PDMS mixing stirring module, the PDMS mixing stirring module comprises a stirring paddle pneumatic motor, a driving gear, a driven gear, a hollow shaft and a stirring paddle, the driving gear, the driven gear, the hollow shaft and the stirring paddle are sequentially connected with the stirring paddle pneumatic motor, the driving gear is in meshed transmission with the driven gear, the driven gear is installed at the top end of the hollow shaft, the stirring paddle is installed at the bottom end of the hollow shaft, and the stirring paddle pneumatic motor is connected with a pressure controller which is used for applying pressure to the stirring paddle pneumatic motor.
3. A PDMS runner system according to claim 1, characterised in that: the extrusion device comprises an extrusion nozzle, a valve core cylinder and a valve core, wherein the valve core penetrates one end of a hollow shaft to be connected with the valve core cylinder, the other end of the hollow shaft is connected with a discharge port of a mixing barrel, the valve core cylinder is connected with a pressure controller and used for controlling the covering state of the valve core and the discharge port, the extrusion nozzle is connected with a discharge port pipeline of the mixing barrel, a mixing barrel extrusion flowmeter is arranged on the pipeline and connected with a computer, and the mixing barrel extrusion flowmeter is used for setting and monitoring the discharge amount of the mixing barrel.
4. A PDMS runner system according to claim 1, characterised in that: the storing tank includes PDMS prepolymer storage tank and PDMS curing agent storage tank, and the storage tank flowmeter includes PDMS prepolymer flowmeter and PDMS curing agent flowmeter, links to each other with the computer respectively for the discharge amount of setting and monitoring PDMS prepolymer and PDMS curing agent.
5. A PDMS runner system according to claim 1, characterised in that: the output end of the pressure controller is connected with the mixing barrel and used for applying negative pressure to the mixing barrel and eliminating bubbles of PDMS mixed liquid in the mixing barrel.
6. A PDMS runner system according to claim 1, characterised in that: the pressure controller is provided with a plurality of positive pressure output ends and a plurality of negative pressure output ends, and a software program for controlling the output state of the pressure controller is arranged in the computer.
7. A PDMS casting method for manufacturing microfluidic chips, based on the PDMS casting system of any one of claims 1 to 6, comprising the following steps:
preparing raw materials, filling a PDMS prepolymer and a PDMS curing agent, setting a positive pressure value of a pressure controller, and applying positive pressure to a storage tank to realize that the PDMS prepolymer and the PDMS curing agent are respectively conveyed to a mixing barrel;
stirring the PDMS mixed liquid, applying positive pressure to a stirring paddle pneumatic motor by a pressure controller, and starting the stirring paddle pneumatic motor so as to drive the stirring paddle to rotate and realize stirring of the PDMS mixed liquid in the mixing barrel; when the PDMS mixed liquid in the mixing barrel is uniformly stirred, the pressure transmission channel is closed, and the stirring paddle stops stirring;
defoaming the PDMS mixed solution, applying negative pressure to the mixing barrel by using a pressure controller, performing bubble removal treatment on the PDMS mixed solution, and closing a pressure conveying channel after the PDMS mixed solution is defoamed;
extruding the PDMS mixed liquid, applying positive pressure to a valve core cylinder by a pressure controller, lifting the valve core to expose a discharge hole positioned at the bottom of a mixing barrel, and applying positive pressure to the mixing barrel by the pressure controller to extrude the PDMS mixed liquid from an extrusion nozzle;
and (3) PDMS pouring, wherein the three-dimensional motion platform is controlled by the computer to enable the extrusion nozzle to move above the chip die, so that the precise pouring of the PDMS mixed liquid is realized.
8. A PDMS pouring method according to claim 7, wherein when the discharging amount of the storage tank reaches a set value, the flow meter of the storage tank feeds back a turn-off signal to the computer, and the computer controls the pressure controller to close the positive pressure channel, thereby stopping the feeding of the PDMS prepolymer and the PDMS curing agent.
9. A PDMS casting method according to claim 7, wherein when the extrusion amount reaches a set value during PDMS casting, the mixing barrel extrusion flowmeter feeds back a shut-off signal to the computer, and the computer controls the pressure controller to close the positive pressure channel, thereby stopping the extrusion of the PDMS mixture.
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