CN115266681A - High-precision scanning and rapid marking method for medical Raman spectral imaging - Google Patents
High-precision scanning and rapid marking method for medical Raman spectral imaging Download PDFInfo
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Abstract
The invention provides a high-precision scanning and rapid marking method for medical Raman spectral imaging, which relates to the technical field of spectral detection, and comprises the following steps: step A10, firstly, obtaining Raman spectrum data of qualified detection medicines from a spectrum database, and classifying the Raman spectrum data of the qualified detection medicines; step A20, setting a basic mark for the detection medicine in the transmission process, and performing Raman spectrum detection for the detection medicine based on the setting of the basic mark; step A30, acquiring real-time Raman spectrum data of a detected drug by adopting a Raman spectrum detector; according to the invention, through carrying out Raman spectrum analysis on the detection medicine and carrying out detailed marking on the detection medicine, the corresponding detection medicine can be quickly tracked in the detection process, so that the problem that the tracking marking is not quick and accurate enough when the medicine is detected by the existing Raman spectrum is solved.
Description
Technical Field
The invention relates to the technical field of spectrum detection, in particular to a high-precision scanning and rapid marking method for medical Raman spectrum imaging.
Background
Raman spectroscopy, which is a scattering spectrum. The raman spectroscopy analysis method is an analysis method for analyzing scattering spectra with different incident light frequencies to obtain information on molecular vibration and rotation based on a raman scattering effect found by indian scientists c.v. raman, and is applied to molecular structure research. The Raman imaging technology is a new generation of rapid, high-precision and surface scanning laser Raman technology, and the confocal microscope technology and the laser Raman spectrum technology are perfectly combined, so that high-fraction Raman images of a sample can be obtained within several minutes generally.
In the prior art, in the field of medical drug detection, for example, when detecting the concentration of a drug in blood, raman spectrum detection is adopted, and reference may be made to patent document CN 201911250494.8; meanwhile, when the components of the medicine are analyzed, a Raman spectrum detection mode is adopted, and the method can be specifically carried out by referring to the following application numbers: CN 201610425006.2; however, the existing detection mode lacks a rapid marking method, so that after the raman spectrum analysis comparison is performed on the detected drugs, the labeling and screening of unqualified products are inconvenient, and a high-precision scanning rapid marking method for medical raman spectrum imaging is lacked to solve the existing problems.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a high-precision scanning and rapid marking method for medical Raman spectrum imaging, which can rapidly track the corresponding detection medicine in the detection process by performing Raman spectrum analysis on the detection medicine and carefully marking the detection medicine, so as to solve the problem that the tracking and marking are not rapid and accurate enough in the medicine detection process by the existing Raman spectrum.
In order to realize the purpose, the invention is realized by the following technical scheme: a high-precision scanning rapid marking method for medical Raman spectrum imaging comprises the following steps:
step A10, firstly, obtaining Raman spectrum data of qualified detection medicines from a spectrum database, and classifying the Raman spectrum data of the qualified detection medicines;
step A20, setting a basic mark for the detection medicine in the transmission process, and performing Raman spectrum detection for the detection medicine based on the setting of the basic mark;
step A30, acquiring real-time Raman spectrum data of the detection medicine by adopting a Raman spectrum detector, comparing the real-time Raman spectrum data with the Raman spectrum data of the qualified detection medicine in a spectrum database, and outputting a detection result according to a comparison result;
and A40, in the process of detecting the drug transmission, marking and tracking the detected drug, and accurately marking the unqualified detected drug based on the detection result.
Further, the step a10 further includes the steps of:
step A101, dividing Raman spectrum data into Raman scattering intensity data and Raman depolarization data;
step A102, comparing and extracting the Raman scattering intensity data and the Raman depolarization data respectively.
Further, the step a102 further includes the following steps:
step a10211, acquiring a raman shift amount of a qualified detection drug at a first incident light wavelength, and setting the raman shift amount as a raman reference shift amount;
step a10212, acquiring raman scattering intensity of a qualified detection drug at a first incident light wavelength, and setting the raman scattering intensity as raman reference scattering intensity;
step A10213, making a Raman reference spectrogram according to the Raman reference displacement and the Raman reference scattering intensity;
step A10214, obtaining a plurality of reference peak points in a Raman reference spectrogram, and then respectively selecting basic reference points of a first reference quantity at intervals of first Raman displacement on two sides of the plurality of reference peak points by taking the first Raman displacement as a unit; acquiring Raman reference scattering intensity on each basic reference point, and substituting the acquired Raman reference scattering intensity on a plurality of basic reference points into a peak reference sharpness calculation formula to obtain a peak sharpness reference value; the peak reference sharpness calculationThe formula is configured as follows:
(ii) a Where Cjd is the peak sharpness reference value, ccps f Ccps1 as the Raman reference scattering intensity at the reference peak point 1 To Ccps1 i Respectively, the Raman reference scattering intensity of a plurality of base reference points on the reference peak point side, ccps2 1 To Ccps2 i Respectively referring to Raman reference scattering intensities of a plurality of basic reference points on the other side of the peak point, wherein i is a first reference number;
step a10215, an average of peak sharpness reference values of the plurality of reference peak points is obtained and set as the peak sharpness reference average.
Further, the step a102 further includes the following steps:
step a10221, obtaining a raman depolarization ratio of the qualified test drug at the first incident light wavelength, and setting the raman depolarization ratio as a raman reference depolarization ratio.
Further, the step a20 further includes the steps of:
step A201, an input detection sensor, an intermediate detection sensor and a tracking detection sensor are sequentially arranged according to the transmission direction of the detected medicine, the input detection sensor and the intermediate detection sensor are separated by a first detection distance, the intermediate detection sensor and the tracking detection sensor are separated by a second detection distance, and the second detection distance is larger than the first detection distance; the Raman spectrum detector is arranged at the middle position of the middle detection sensor and the tracking detection sensor;
step A202, marking the detection medicines as Yj in sequence according to the transmission direction 1 To Yj n Yj is a mark for detecting the medicine, and 1 to n are marks for detecting the medicine;
step A203, acquiring the transmission speed and the transmission interval of the detection medicines, wherein the transmission interval is the distance between every two detection medicines;
step A204, substituting the transmission speed and the first detection distance into a detection starting calculation formula to obtain a detection starting duration; when the first detection drug delivery time reaches the detection start time and the middle detection sensingWhen the number of the detection medicines detected by the device is 1, outputting a basic detection starting signal; the detection turn-on calculation formula is configured to:
(ii) a Wherein Tjk is the detection starting time length, sj1 is the first detection distance, and Vcs is the transmission speed;
step A205, substituting the second detection distance and the transmission speed into a Raman detection starting formula to obtain Raman detection duration; outputting a Raman detection starting signal after a Raman detection duration is spaced when a basic detection starting signal is received; the raman detection initiation formula is configured to:
(ii) a Wherein, tlm is the Raman detection duration, and Sj2 is the second detection distance.
Further, the step a30 further includes the following steps:
step A3011, substituting the transmission interval and the transmission speed into a detection interval calculation formula to obtain a detection pause duration; the detection interval calculation formula is configured to:
(ii) a When receiving a Raman detection starting signal, starting a Raman spectrum detector to detect the pause duration at each interval and carrying out detection once by adopting a first incident light wavelength;
step A3012, setting the detected Raman displacement of the detected drug as Raman real-time displacement; setting the detected Raman scattering intensity of the detected drug as Raman real-time scattering intensity;
a3013, making a Raman real-time spectrogram according to the Raman real-time displacement and the Raman real-time scattering intensity;
a3014, acquiring a plurality of real-time peak points in a Raman real-time spectrogram, and then selecting real-time reference points of a first reference number at intervals of first Raman displacement on two sides of the plurality of real-time peak points by taking the first Raman displacement as a unit; obtaining each real-time reference pointSubstituting the obtained Raman real-time scattering intensities on the plurality of real-time reference points into a peak real-time sharpness calculation formula to obtain a peak sharpness real-time value; the peak real-time sharpness calculation formula is configured as:
(ii) a Where Sjd is the peak sharpness real-time value, scrs f Scan 1 as Raman real-time Scattering intensity of real-time peak points 1 To Scps1 i Raman real-time scattering intensity, scps2, of several real-time reference points on one side of the real-time peak point, respectively 1 To Scps2 i Respectively the Raman real-time scattering intensity of a plurality of real-time reference points on the other side of the real-time peak point;
step A3015, calculating an average value of peak sharpness real-time values of the plurality of real-time peak points, and setting the average value as a peak sharpness real-time average value;
step A3016, setting the Raman depolarization ratio of the detected detection drug as the Raman real-time depolarization ratio.
Further, the step a30 further includes the following steps:
step A3021, substituting the reference average value of the peak sharpness and the real-time average value of the peak sharpness into a first comparison formula to obtain a first comparison difference value; when the first comparison difference value is larger than or equal to the first comparison threshold value, outputting a first early warning signal; the first comparison formula is configured to:
(ii) a Where Pc1 is a first comparison difference, cjd avg Peak sharpness reference mean, sjd avg The peak sharpness real-time average value is obtained;
step A3022, substituting the Raman reference depolarization ratio and the Raman real-time depolarization ratio into a second comparison formula to obtain a second comparison difference; when the second comparison difference value is larger than or equal to the second comparison threshold value, outputting a second early warning signal; the second alignment formula is configured to:
(ii) a Wherein Pc2 is the second comparison differenceThe value of the sum of the values,
for the raman reference depolarization ratio,
the Raman real-time depolarization ratio is obtained;
step A3023, adding the first comparison difference and the second comparison difference to obtain a comprehensive reference early warning value; and outputting a comprehensive early warning signal when the comprehensive reference early warning value is greater than or equal to the first comprehensive comparison threshold value.
Further, the step a40 further includes the following steps:
step A401, when a first early warning signal, a second early warning signal and a comprehensive early warning signal are received, a mark tracking signal is started;
step A402, acquiring a label of a detection drug corresponding to a generated label tracking signal, and setting the label as an early warning tracking label; then acquiring a starting time point of the mark tracking signal, and setting the starting time point as a tracking starting point; then acquiring a starting time point of the Raman spectrum detector, setting the starting time point as a detection starting point, and subtracting the time point of the detection starting point from the time point of the tracking starting point to obtain a first time difference;
step A403, obtaining a tracking duration through a tracking interval calculation formula; the tracking interval calculation formula is configured to:
(ii) a Wherein, tzj is tracking duration, T1c is a first time difference, and z is an early warning tracking label;
step a404, taking the tracking start point as the tracking start time, and after the tracking duration, acquiring the first detected detection drug at this time by the tracking detection sensor, and marking as the detection tracking drug.
The invention has the beneficial effects that: firstly, acquiring Raman spectrum data of qualified detection medicines from a spectrum database, and classifying the Raman spectrum data of the qualified detection medicines; acquiring real-time Raman spectrum data of the detection medicine by adopting a Raman spectrum detector, comparing the real-time Raman spectrum data with the Raman spectrum data of the qualified detection medicine in the spectrum database, and outputting a detection result according to a comparison result; the method can rapidly screen whether the detected medicine is qualified or not;
the invention sets the basic mark of the detection medicine in the transmission process, and performs Raman spectrum detection on the detection medicine based on the setting of the basic mark; in the process of detecting the transmission of the medicine, marking and tracking the detected medicine, and accurately marking the unqualified detected medicine based on the detection result; the method can be associated with the detection result, and the marking timeliness and tracking accuracy of unqualified products are improved.
Advantages of additional aspects of the invention will be set forth in part in the description of the embodiments which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
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Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a flow chart of a marking method of the present invention;
FIG. 2 is a schematic partial partitioning diagram of a Raman reference spectrum according to the present invention;
FIG. 3 is a partially partitioned schematic illustration of a Raman real-time spectrum of the present invention;
fig. 4 is a schematic structural arrangement diagram of the detection device of the present invention.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention.
The embodiments and features of the embodiments of the present invention may be combined with each other without conflict.
The invention provides a high-precision scanning and rapid marking method for medical Raman spectrum imaging, which can quickly track corresponding detection medicines in a detection process by performing Raman spectrum analysis on the detection medicines and carefully marking the detection medicines, so as to solve the problem that the tracking and marking are not quick and accurate enough when the existing Raman spectrum is used for medicine detection.
Referring to fig. 1, specifically, the marking method includes the following steps:
step A10, firstly, obtaining Raman spectrum data of qualified detection medicines from a spectrum database, and classifying the Raman spectrum data of the qualified detection medicines; the spectrum database can store the spectrum data corresponding to the qualified products, so that the subsequent real-time detection and comparison are facilitated;
step A20, setting a basic mark for the detection medicine in the transmission process, and performing Raman spectrum detection for the detection medicine based on the setting of the basic mark; the detection drugs are subjected to label setting, so that the drugs can be quickly selected when the drugs need to be screened, and the screening efficiency is improved;
step A30, acquiring real-time Raman spectrum data of the detection medicine by adopting a Raman spectrum detector, comparing the real-time Raman spectrum data with the Raman spectrum data of the qualified detection medicine in a spectrum database, and outputting a detection result according to a comparison result; the difference between the real-time detection data and the pre-stored data can be reflected by the comparison of the Raman spectra, so that accurate screening is realized;
step A40, in the process of detecting the drug transmission, marking and tracking the detected drug, accurately marking the unqualified detected drug based on the detection result, and improving the tracking accuracy and timeliness of the detected drug to be tracked through marking and screening.
Example one
Wherein, step A10 includes the following steps:
a101, dividing Raman spectrum data into Raman scattering intensity data and Raman depolarization ratio data;
step A102, comparing and extracting the Raman scattering intensity data and the Raman depolarization data respectively; step a102 further comprises the steps of:
step A10211, obtaining Raman shift amount of qualified detection drug under the first incident light wavelength, and setting the Raman shift amount as Raman reference shift amount; in the field of raman spectroscopy detection, incident light, after passing through a scattering center, can be classified into stokes raman scattering, rayleigh scattering, and anti-stokes raman scattering, where the stokes line: the frequency of the scattered light is less than the frequency of the incident light; anti-stokes line: the frequency of the scattered light is greater than the frequency of the incident light; the difference of the frequencies is Raman shift, the Raman shift is irrelevant to the frequency of incident light and only relevant to the vibration and rotation energy level of the substance molecules, so that the Raman shift can be used for analyzing and researching the substance structure; setting the incident light with the same wavelength can keep the basic parameters consistent when the alignment is carried out.
Step a10212, acquiring raman scattering intensity of a qualified detection drug at a first incident light wavelength, and setting the raman scattering intensity as raman reference scattering intensity; the Raman scattering intensity is mainly expressed as that the Stokes scattering intensity is stronger than that of anti-Stokes, and is mostly used as signal light for quantitative analysis; the size, intensity and Raman peak shape of Raman shift are important basis for identifying chemical bond and functional group, and Raman spectrum can also be used as basis for judging molecular isomer by utilizing polarization property;
step A10213, making a Raman reference spectrogram according to the Raman reference displacement and the Raman reference scattering intensity;
referring to fig. 2, in step a10214, a plurality of reference peak points in the raman reference spectrogram are obtained, and then, the first raman shift amount is used as a unit, and the base reference points of the first reference number are respectively selected at two sides of the plurality of reference peak points every interval of the first raman shift amount; acquiring Raman reference scattering intensity on each basic reference point, and substituting the acquired Raman reference scattering intensity on a plurality of basic reference points into a peak reference sharpness calculation formula to obtain a peak sharpness reference value; the peak reference sharpness calculation formula is configured as:
(ii) a Where Cjd is the peak sharpness reference value, ccps f Ccps1 as the Raman reference scattering intensity of the reference peak point 1 To Ccps1 i Raman reference scattering intensity, ccps2, of a number of base reference points, one side of the reference peak point 1 To Ccps2 i Respectively the Raman reference scattering intensity of a plurality of basic reference points at the other side of the reference peak point, wherein i is a first reference number; the peak reference sharpness calculation formula can obtain that the higher the peak sharpness reference value is, the faster the image change of the two sides of the reference peak point is used for calculation, namely the sharper the peak sharpness is in image display, by referring to the change condition of the Raman reference scattering intensity of the basic reference points at the two sides of the peak point; substances of different chemical nature exhibit spectral patterns with different peak shapes.
Step a10215, an average of peak sharpness reference values of the plurality of reference peak points is obtained and set as the peak sharpness reference average.
Step a102 further comprises the steps of:
step a10221, obtaining a raman depolarization ratio of the qualified test drug at the first incident light wavelength, and setting the raman depolarization ratio as a raman reference depolarization ratio. In raman scattering measurements, the raman depolarization ratio is an important quantity that provides information about the internal structure of the molecule and its symmetry. Under the condition of non-electronic state resonance, for the fully symmetric vibration mode of the molecule, the vibration mode is not less than 0
<0.75; for a non-fully symmetric or degenerate vibrational mode,
=0.75, therefore, we can judge the symmetry of the molecular vibrational mode by the magnitude of the raman depolarization ratio.
Step a20 further comprises the steps of:
referring to fig. 4, the marking method of the present invention is configured with a detection device, which includes an input detection sensor, an intermediate detection sensor, a tracking detection sensor, and a raman spectrum detector. Step A201, sequentially arranging an input detection sensor, an intermediate detection sensor and a tracking detection sensor according to the transmission direction of the detected medicine, wherein the distance between the input detection sensor and the intermediate detection sensor is a first detection distance, the distance between the intermediate detection sensor and the tracking detection sensor is a second detection distance, and the second detection distance is larger than the first detection distance; wherein the Raman spectrum detector is arranged at the middle position between the middle detection sensor and the tracking detection sensor; the input detection sensor, the middle detection sensor and the tracking detection sensor have different functions, the input detection sensor is used for acquiring the entry information of the detected medicine, the middle detection sensor is used for calibrating whether the detected medicine is normally transmitted or not, the distance between the middle detection sensor and the tracking detection sensor is long enough, on one hand, the middle detection sensor can be used for transmitting the detected medicine, and on the other hand, the required reaction time for tracking can be reserved when the detection result of the detected medicine is abnormal; the input detection sensor, the intermediate detection sensor and the tracking detection sensor preferably employ an electro-optical proximity sensor.
Step A202, marking the detection medicines as Yj in sequence according to the transmission direction 1 To Yj n Yj is a mark for detecting the medicine, and 1 to n are marks for detecting the medicine; for example, when the number of test drugs to be tested is 100, n =100;
step A203, acquiring the transmission speed and the transmission interval of the detection medicines, wherein the transmission interval is the distance between every two detection medicines; the transmission intervals between each two groups of test drugs are generally equal;
step A204, substituting the transmission speed and the first detection distance into a detection starting calculation formula to obtain a detection starting time length; outputting a basic detection starting signal when the first detection medicine transmission time reaches the detection starting time and the quantity of the detection medicines detected by the middle detection sensor is 1; the detection on calculation formula is configured as:
(ii) a Wherein, tjk is the detection starting time length, sj1 is the first detection distance, and Vcs is the transmission speed; input detection sensor and intermediate detection sensorThe first detection distance between the devices is used for providing a starting reaction time for the Raman spectrum detector;
step A205, substituting the second detection distance and the transmission speed into a Raman detection starting formula to obtain a Raman detection duration; outputting a Raman detection starting signal after a Raman detection duration is spaced when a basic detection starting signal is received; the raman detection initiation formula is configured as:
(ii) a Wherein, tlm is the Raman detection duration, and Sj2 is the second detection distance. The raman spectroscopy detector is disposed intermediate the input detection sensor and the intermediate detection sensor such that the raman detection period is also exactly the time that the first test agent is transferred intermediate the input detection sensor and the intermediate detection sensor.
Step a30 further comprises the steps of:
step A3011, substituting the transmission interval and the transmission speed into a detection interval calculation formula to obtain a detection pause duration; the detection interval calculation formula is configured as:
(ii) a Wherein, tjc is the detection pause duration, scj is the transmission interval, when receiving a Raman detection starting signal, the Raman spectrum detector is started to detect the pause duration at each interval and adopts the first incident light wavelength to carry out detection once;
step A3012, setting the detected Raman displacement of the detected drug as Raman real-time displacement; setting the detected Raman scattering intensity of the detected drug as Raman real-time scattering intensity;
step A3013, making a Raman real-time spectrogram according to the Raman real-time displacement and the Raman real-time scattering intensity;
referring to fig. 3, in step a3014, a plurality of real-time peak points in the raman real-time spectrogram are obtained, and then, with the first raman shift amount as a unit, real-time reference points of a first reference number are respectively selected at two sides of the plurality of real-time peak points every interval of the first raman shift amount; acquiring the Raman real-time scattering intensity at each real-time reference point,substituting the obtained Raman real-time scattering intensity on a plurality of real-time reference points into a peak real-time sharpness calculation formula to obtain a peak sharpness real-time value; the peak real-time sharpness calculation formula is configured as:
(ii) a Wherein Sjd is the peak sharpness real-time value, scps f Scan real-time Scan intensity for real-time peak points, scps1 1 To Scps1 i Raman real-time scattering intensity, scps2, of several real-time reference points on one side of the real-time peak point, respectively 1 To Scps2 i Respectively the Raman real-time scattering intensity of a plurality of real-time reference points on the other side of the real-time peak point;
step A3015, calculating an average value of peak sharpness real-time values of the plurality of real-time peak points, and setting the average value as a peak sharpness real-time average value;
step A3016, setting the detected Raman depolarization ratio of the detected drug as a Raman real-time depolarization ratio, and in the real-time detection process, corresponding the analyzed and compared data to the data in the spectrum database one by one.
Step a30 further comprises the steps of:
step A3021, substituting the reference average value of the peak sharpness and the real-time average value of the peak sharpness into a first comparison formula to obtain a first comparison difference value; when the first comparison difference value is larger than or equal to a first comparison threshold value, outputting a first early warning signal; the first comparison formula is configured as:
(ii) a Where Pc1 is a first comparison difference, cjd avg Peak sharpness reference mean, sjd avg The peak sharpness real-time average value is obtained; step A3022, substituting the Raman reference depolarization ratio and the Raman real-time depolarization ratio into a second comparison formula to obtain a second comparison difference; when the second comparison difference value is larger than or equal to the second comparison threshold value, outputting a second early warning signal; the second alignment formula is configured as:
(ii) a Wherein Pc2 is the second comparison differenceThe value of the sum of the values,
for the raman reference depolarization ratio,
the Raman real-time depolarization ratio is obtained; step A3022, adding the first comparison difference and the second comparison difference to obtain a comprehensive reference early warning value; and when the comprehensive reference early warning value is greater than or equal to the first comprehensive comparison threshold value, outputting a comprehensive early warning signal. In the detection method for the detection medicine, the obtained Raman data is only used as comparison reference data, qualified data and real-time detected data are compared, and if the comparison error is large, the detection medicine is marked.
Step a40 further comprises the steps of:
step A401, when a first early warning signal, a second early warning signal and a comprehensive early warning signal are received, a mark tracking signal is started;
step A402, acquiring a label of a detection drug corresponding to a generated label tracking signal, and setting the label as an early warning tracking label; then acquiring a starting time point of the mark tracking signal, and setting the starting time point as a tracking starting point; then acquiring a starting time point of the Raman spectrum detector, setting the starting time point as a detection starting point, and subtracting the time point of the detection starting point from the time point of the tracking starting point to obtain a first time difference;
step A403, obtaining a tracking duration through a tracking interval calculation formula; the tracking interval calculation formula is configured as:
(ii) a Wherein, tzj is tracking duration, T1c is a first time difference, and z is an early warning tracking label;
step A404, taking the tracking starting point as the tracking starting time, and after the tracking duration, acquiring the first detected detection drug at the moment through the tracking detection sensor, and marking the first detected detection drug as the detection tracking drug.
The above formulas are all the formulas for taking the numerical value of the dimension, the formula is a formula for obtaining the latest real situation by software simulation of collected mass data, the preset parameters in the formula are set by the technical personnel in the field according to the actual situation, if the weight coefficient and the proportion coefficient exist, the set size is a specific numerical value obtained by quantizing each parameter, the subsequent comparison is convenient, and the size of the weight coefficient and the proportion coefficient can be obtained as long as the proportional relation between the parameter and the quantized numerical value is not influenced.
In the above embodiments of the present application, the descriptions of the respective embodiments have respective emphasis, and for parts that are not described in detail in a certain embodiment, reference may be made to related descriptions of other embodiments.
As will be appreciated by one skilled in the art, embodiments of the present invention may be provided as a method, system, or computer program product. Accordingly, the present invention may take the form of an entirely hardware embodiment, an entirely software embodiment or an embodiment combining software and hardware aspects. Furthermore, the present invention may take the form of a computer program product embodied on one or more computer-usable storage media having computer-usable program code embodied in the medium. The storage medium may be implemented by any type of volatile or nonvolatile storage device or combination thereof, such as a Static Random Access Memory (SRAM), an Electrically Erasable Programmable Read-Only Memory (EEPROM), an Erasable Programmable Read-Only Memory (EPROM), a Programmable Read-Only Memory (PROM), an on-Read Memory (ROM), a magnetic Memory, a flash Memory, a magnetic disk, or an optical disk. These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including instruction means which implement the function specified in the flowchart flow or flows and/or block diagram block or blocks.
Although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that the following descriptions are only illustrative and not restrictive, and that the scope of the present invention is not limited to the above embodiments: those skilled in the art can still make modifications or changes to the embodiments described in the foregoing embodiments, or make equivalent substitutions for some features, within the scope of the disclosure; such modifications, changes or substitutions do not depart from the spirit and scope of the embodiments of the present invention, and they should be construed as being included therein. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (8)
1. A high-precision scanning rapid marking method for medical Raman spectrum imaging is characterized by comprising the following steps:
step A10, firstly, obtaining Raman spectrum data of qualified detection medicines from a spectrum database, and classifying the Raman spectrum data of the qualified detection medicines;
step A20, setting a basic mark for the detection medicine in the transmission process, and performing Raman spectrum detection for the detection medicine based on the setting of the basic mark;
step A30, acquiring real-time Raman spectrum data of the detection medicine by adopting a Raman spectrum detector, comparing the real-time Raman spectrum data with the Raman spectrum data of the qualified detection medicine in a spectrum database, and outputting a detection result according to a comparison result;
and A40, marking and tracking the detection medicines in the transmission process of the detection medicines, and accurately marking the unqualified detection medicines based on the detection result.
2. The method for scanning and marking rapidly with high precision for medical raman spectroscopic imaging according to claim 1, wherein said step a10 further comprises the steps of:
a101, dividing Raman spectrum data into Raman scattering intensity data and Raman depolarization ratio data;
step A102, comparing and extracting the Raman scattering intensity data and the Raman depolarization data respectively.
3. The method for scanning and rapidly marking with high precision of medical raman spectroscopy imaging according to claim 2, wherein the step a102 further comprises the steps of:
step a10211, acquiring a raman shift amount of a qualified detection drug at a first incident light wavelength, and setting the raman shift amount as a raman reference shift amount;
step a10212, acquiring raman scattering intensity of a qualified detection drug at a first incident light wavelength, and setting the raman scattering intensity as raman reference scattering intensity;
step A10213, making a Raman reference spectrogram according to the Raman reference displacement and the Raman reference scattering intensity;
step A10214, obtaining a plurality of reference peak points in a Raman reference spectrogram, and then respectively selecting basic reference points of a first reference quantity at intervals of first Raman displacement on two sides of the plurality of reference peak points by taking the first Raman displacement as a unit; acquiring Raman reference scattering intensity on each basic reference point, and substituting the acquired Raman reference scattering intensity on a plurality of basic reference points into a peak reference sharpness calculation formula to obtain a peak sharpness reference value; the peak reference sharpness calculation formula is configured to:
where Cjd is the peak sharpness reference value, ccps f Ccps1 as the Raman reference scattering intensity of the reference peak point 1 To Ccps1 i Raman reference scattering intensity, ccps2, of a number of base reference points, one side of the reference peak point 1 To Ccps2 i Raman references respectively referring to a plurality of base reference points on the other side of the peak pointScattering intensity, i is a first reference quantity;
step a10215, an average of peak sharpness reference values of the reference peak points is obtained and set as the peak sharpness reference average.
4. The method for scanning and rapidly marking with high precision of medical Raman spectrum imaging according to claim 3, wherein said step A102 further comprises the steps of:
step a10221, obtaining a raman depolarization ratio of the qualified test drug at the first incident light wavelength, and setting the raman depolarization ratio as a raman reference depolarization ratio.
5. The method for scanning and rapidly marking with high precision in medical Raman spectrum imaging according to claim 4, wherein the step A20 further comprises the following steps:
step A201, an input detection sensor, an intermediate detection sensor and a tracking detection sensor are sequentially arranged according to the transmission direction of the detected medicine, the input detection sensor and the intermediate detection sensor are separated by a first detection distance, the intermediate detection sensor and the tracking detection sensor are separated by a second detection distance, and the second detection distance is larger than the first detection distance; wherein the Raman spectrum detector is arranged at the middle position between the middle detection sensor and the tracking detection sensor;
step A202, marking the detection medicines as Yj according to the transmission direction in sequence 1 To Yj n Yj is a label for detecting the drug, and 1 to n are labels for detecting the drug;
step A203, acquiring the transmission speed and the transmission interval of the detection medicines, wherein the transmission interval is the distance between every two detection medicines;
step A204, substituting the transmission speed and the first detection distance into a detection starting calculation formula to obtain a detection starting time length; outputting a basic detection starting signal when the first detection medicine transmission time reaches the detection starting time and the quantity of the detection medicines detected by the middle detection sensor is 1; the detection turn-on calculation formula is configured to:
(ii) a Wherein, tjk is the detection starting time length, sj1 is the first detection distance, and Vcs is the transmission speed;
step A205, substituting the second detection distance and the transmission speed into a Raman detection starting formula to obtain a Raman detection duration; outputting a Raman detection starting signal after a Raman detection duration is spaced when a basic detection starting signal is received; the raman detection initiation formula is configured to:
(ii) a Wherein, tlm is the Raman detection duration, and Sj2 is the second detection distance.
6. The method for scanning and rapidly marking with high precision in medical Raman spectrum imaging according to claim 5, wherein the step A30 further comprises the following steps:
step A3011, substituting the transmission interval and the transmission speed into a detection interval calculation formula to obtain a detection pause duration; the detection interval calculation formula is configured to:
(ii) a Wherein, tjc is the detection pause duration, scj is the transmission interval, when receiving a Raman detection starting signal, the Raman spectrum detector is started to detect the pause duration at each interval and adopts the first incident light wavelength to carry out detection once;
step A3012, setting the detected Raman displacement of the detected drug as Raman real-time displacement; setting the detected Raman scattering intensity of the detection medicine as Raman real-time scattering intensity;
a3013, making a Raman real-time spectrogram according to the Raman real-time displacement and the Raman real-time scattering intensity;
step A3014, obtaining a plurality of real-time peak points in the Raman real-time spectrogram, and then selecting a first reference number of real Raman shifts at each interval on two sides of the plurality of real-time peak points by taking the first Raman shifts as a unitA temporal reference point; acquiring Raman real-time scattering intensity on each real-time reference point, and substituting the acquired Raman real-time scattering intensity on a plurality of real-time reference points into a peak value real-time sharpness calculation formula to obtain a peak value real-time value; the peak real-time sharpness calculation formula is configured as:
(ii) a Wherein Sjd is the peak sharpness real-time value, scps f Scan real-time Scan intensity for real-time peak points, scps1 1 To Scps1 i Raman real-time scattering intensity, scps2, of several real-time reference points on one side of the real-time peak point, respectively 1 To Scps2 i Respectively the Raman real-time scattering intensity of a plurality of real-time reference points at the other side of the real-time peak point;
step A3015, calculating an average of the real-time peak value values of the plurality of real-time peak value points, and setting the average as the real-time peak value average;
step A3016, setting the Raman depolarization ratio of the detected detection drug as the Raman real-time depolarization ratio.
7. The method for scanning and rapidly marking with high precision in medical Raman spectrum imaging according to claim 6, wherein the step A30 further comprises the following steps:
step A3021, substituting the reference average value of the peak sharpness and the real-time average value of the peak sharpness into a first comparison formula to obtain a first comparison difference value; when the first comparison difference value is larger than or equal to the first comparison threshold value, outputting a first early warning signal; the first comparison formula is configured to:
(ii) a Wherein Pc1 is a first comparison difference, cjd avg Peak sharpness reference mean, sjd avg The peak sharpness real-time average value is obtained;
step A3022, substituting the Raman reference depolarization ratio and the Raman real-time depolarization ratio into a second comparison formula to obtain a second comparison difference; when the second comparison difference is greater than or equal to the second comparison thresholdWhen the value is positive, outputting a second early warning signal; the second alignment formula is configured to:
(ii) a Wherein Pc2 is the second comparison difference,
for the raman reference depolarization ratio,
the Raman real-time depolarization ratio is obtained;
step A3023, adding the first comparison difference and the second comparison difference to obtain a comprehensive reference early warning value; and outputting a comprehensive early warning signal when the comprehensive reference early warning value is greater than or equal to the first comprehensive comparison threshold value.
8. The method for scanning and rapidly marking with high precision of medical raman spectroscopy imaging according to claim 7, wherein the step a40 further comprises the steps of:
step A401, when a first early warning signal, a second early warning signal and a comprehensive early warning signal are received, a marking tracking signal is started;
step A402, acquiring a label of a detection drug corresponding to a generated label tracking signal, and setting the label as an early warning tracking label; then acquiring a starting time point of the mark tracking signal, and setting the starting time point as a tracking starting point; then acquiring a starting time point of the Raman spectrum detector, setting the starting time point as a detection starting point, and subtracting the time point of the detection starting point from the time point of the tracking starting point to obtain a first time difference;
step A403, obtaining a tracking duration through a tracking interval calculation formula; the tracking interval calculation formula is configured to:
(ii) a Wherein, tzj is tracking duration, T1c is a first time difference, and z is an early warning tracking label;
step A404, taking the tracking starting point as the tracking starting time, and after the tracking duration, acquiring the first detected detection drug at the moment through the tracking detection sensor, and marking the first detected detection drug as the detection tracking drug.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070076161A (en) * | 2006-01-18 | 2007-07-24 | 주식회사 로보스타 | Tracking method and system for moving object of robot |
| US20100291599A1 (en) * | 2009-05-18 | 2010-11-18 | Bruker Optics, Inc. | Large area scanning apparatus for analyte quantification by surface enhanced raman spectroscopy and method of use |
| CN106596499A (en) * | 2016-11-01 | 2017-04-26 | 北京华泰诺安技术有限公司 | Real-time Raman spectrum calibration method |
| CN110530869A (en) * | 2018-05-25 | 2019-12-03 | 上海翌视信息技术有限公司 | A kind of detection system based on location information and image information |
| CN111141719A (en) * | 2019-12-27 | 2020-05-12 | 梧州市食品药品检验所 | Rapid nondestructive identification method for anti-AIDS medicine |
| CN111638202A (en) * | 2020-06-04 | 2020-09-08 | 清华大学 | Double-optical-comb coherent anti-Stokes Raman spectrum detection system for localized rapid time-delay scanning |
| CN114119676A (en) * | 2022-01-24 | 2022-03-01 | 西安羚控电子科技有限公司 | Target detection tracking identification method and system based on multi-feature information fusion |
-
2022
- 2022-09-27 CN CN202211177761.5A patent/CN115266681B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070076161A (en) * | 2006-01-18 | 2007-07-24 | 주식회사 로보스타 | Tracking method and system for moving object of robot |
| US20100291599A1 (en) * | 2009-05-18 | 2010-11-18 | Bruker Optics, Inc. | Large area scanning apparatus for analyte quantification by surface enhanced raman spectroscopy and method of use |
| CN106596499A (en) * | 2016-11-01 | 2017-04-26 | 北京华泰诺安技术有限公司 | Real-time Raman spectrum calibration method |
| CN110530869A (en) * | 2018-05-25 | 2019-12-03 | 上海翌视信息技术有限公司 | A kind of detection system based on location information and image information |
| CN111141719A (en) * | 2019-12-27 | 2020-05-12 | 梧州市食品药品检验所 | Rapid nondestructive identification method for anti-AIDS medicine |
| CN111638202A (en) * | 2020-06-04 | 2020-09-08 | 清华大学 | Double-optical-comb coherent anti-Stokes Raman spectrum detection system for localized rapid time-delay scanning |
| CN114119676A (en) * | 2022-01-24 | 2022-03-01 | 西安羚控电子科技有限公司 | Target detection tracking identification method and system based on multi-feature information fusion |
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