CN115252466A

CN115252466A - Application of polysaccharides and derivatives thereof in freckle removing and whitening products

Info

Publication number: CN115252466A
Application number: CN202211008550.9A
Authority: CN
Inventors: 段仰凯; 李新; 李祎宸
Original assignee: Qingdao Zhongke Lanzhi Biotechnology Development Co ltd
Current assignee: Qingdao Zhongke Lanzhi Biotechnology Development Co ltd
Priority date: 2022-08-22
Filing date: 2022-08-22
Publication date: 2022-11-01

Abstract

The invention discloses application of polysaccharide and derivatives thereof in freckle removing and whitening products, wherein the content of glycerol glucoside in the freckle removing and whitening products is 0.01-5%, and the glycerol glucoside with the weight percentage of 0.5-3% and the three-dimensional configuration of 2-alpha GG is applied to the freckle removing and whitening products, so that the composition can penetrate into the dermis of skin, the moisture can be supplemented to the dermis of the skin, and the freckle removing and whitening products have the long-acting moisturizing effect. And the combination of the evening primrose oil, the olive oil and the macadamia nut oil can ensure that the composition has the effect of repairing skin barriers, and particularly the weight ratio of the evening primrose oil to the macadamia nut oil is 32:13:22, the composition can promote skin protein expression and reduce skin sensitivity after long-term use. Meanwhile, the plant extract and the glycerol glucoside have synergistic effect, so that the compatibility among all components in the composition can be improved, the composition can be quickly absorbed by skin when in use, and the greasy feeling of the skin is reduced.

Description

Application of polysaccharides and derivatives thereof in freckle removing and whitening products

Technical Field

The invention relates to application of polysaccharides and derivatives thereof in freckle removing and whitening products, and belongs to the field of application of polysaccharides and derivatives thereof.

Background

Polysaccharides and derivatives thereof are compounds formed by connecting segments by glycosidic bonds and polymerizing a plurality of single substances, and different products can be obtained according to different ligands, and common polysaccharides and derivatives thereof include cellulose, starch, glycogen, glycoside and the like.

The glycerol glucoside is a compound formed by connecting glycerol and glucose through chemical bonds, has various stereo structures according to different stereo configurations and connecting positions of glycosidic bonds, has various preparation methods, has different configurations and purities, and can also cause different molecular weights of the purified glycerol glucoside, thereby generating different effects in products. However, there is still a lack of effective and useful application products for the application of glycerol glucoside.

The Chinese invention patent CN202011505232.4 discloses a moisturizing mask liquid containing glycerol glucoside and a preparation method thereof, and the traditional moisturizing raw material is replaced by the glycerol glucoside, so that the moisturizing absorption efficiency is enhanced, the use feeling is improved, the moisturizing effect of the glycerol glucoside is emphasized, and the repairing effect is not involved. Chinese invention patent CN202110149721.9 discloses a starch-based skin repairing film and a preparation method thereof, wherein glycerol glucoside is used as a humectant, and is matched with an anti-allergy component and an anti-inflammatory drug component to realize the repairing effect, but the adopted drug anti-inflammatory and antibacterial components are used for the face to easily cause skin allergy, and must be matched with an anti-allergy component to use, so that the irritation is strong.

Disclosure of Invention

In order to obtain a freckle removing and whitening product which is mild in action effect and has a repairing and barrier effect on skin and is introduced with the glycerol glucoside, the invention provides an application of polysaccharides and derivatives thereof in the freckle removing and whitening product, wherein the content of the glycerol glucoside in the freckle removing and whitening product is 0.01-10%;

as a preferred embodiment, the application of the freckle removing and whitening product is selected from one of skin lotion, skin milk, skin cream, essence, ampoules, facial masks, inferior polish and eye cream.

In a preferred embodiment, the polysaccharide and the derivative thereof are at least one of starch, cellulose, glycogen, and glycoside.

As a preferred embodiment, the glycoside is glycerol glucoside; the spatial configuration of the glycerol glucoside is any one of 2-alpha GG and 2-beta GG.

In a preferred embodiment, the glycerol glucoside is extracted from algae cells of blue algae.

As a preferred embodiment, the spot-removing whitening product comprises one of whitening, acne-removing, barrier-repairing, anti-aging, anti-inflammatory, moisturizing, pore-refining spot-removing whitening products.

As a preferred embodiment, the weight percentage of the glycerol glucoside in the freckle removing and whitening product is 0.5% -3%; the spatial configuration of the glycerol glucoside is 2-alpha GG.

As a preferred embodiment, the raw materials for preparing the freckle removing and whitening product comprise the following components in percentage by weight: 0.5-3% of glycerol glucoside, 1-15% of plant extract, 10-30% of vegetable oil composition, 0.1-5% of peptide compound, 10-40% of small molecular organic alcohol, 10-20% of skin conditioner and the balance of deionized water.

As a preferred embodiment, the plant extract is selected from one or more of seaweed extract, aloe extract, centella asiatica extract, salvia miltiorrhiza extract, grapefruit extract, hawthorn extract, chamomile extract, and witch hazel extract.

As a preferred embodiment, the plant extract is a combination of the seaweed sargasumpallidum (turn.) c. Ag extract, aloe vera extract, centella asiatica (l.) Urban extract.

As a preferred embodiment, the weight ratio of the seaweed extract, the aloe extract and the centella asiatica extract is (2-5): (2-3): (1-2).

As a preferred embodiment, the weight ratio of the seaweed extract, the aloe extract and the centella asiatica extract is (3-4.5): (2-2.8): (1.2-1.5).

As a preferred embodiment, the weight ratio of the seaweed extract, the aloe extract and the centella asiatica extract is 4:2.5:1.3.

as a preferred embodiment, the vegetable oil composition is selected from one or more of evening primrose oil, olive oil, macadamia nut oil, borage oil, castor oil, jojoba seed oil, shea butter, camellia oil, and sunflower seed oil.

As a preferred embodiment, the vegetable oil composition is a combination of evening primrose oil, olive oil, macadamia nut oil.

In a preferred embodiment, the weight ratio of the evening primrose oil, the olive oil and the macadamia nut oil is (20-35): (10-20): (20-25).

In a preferred embodiment, the weight ratio of the evening primrose oil, the olive oil and the macadamia nut oil is (25-34): (11-15): (20-24).

In a preferred embodiment, the weight ratio of the evening primrose oil, the olive oil and the macadamia nut oil is 32:13:22.

in the experimental process, the applicant finds that the composition has a long-acting moisturizing effect and also has the function of repairing the stratum corneum barrier by adopting the combination of the plant oil of evening primrose oil, olive oil and macadamia nut oil when the 2-alpha glycerol glucoside is adopted as the stereo configuration and the addition amount is 0.5-3%. The possible reasons for guessing are: the 2-alpha three-dimensional glycerol glucoside is natural glycerol glucan extracted from cultured algae, has small molecular weight, and can penetrate into the dermis of the skin to supplement water for the dermis of the skin. The glycerodextran can promote the expression of aquaporin AQP3 of HaCaT cells, and open a water replenishing channel from a corium layer to a cuticle layer, so that the skin can realize quick water replenishing and can also realize long-acting water replenishing and moisture preservation. And the applicant has further found that, following the combination of vegetable oils with evening primrose oil, olive oil and macadamia nut oil, in particular the weight ratio of evening primrose oil, olive oil and macadamia nut oil is 32:13:22, it is possible to confer a composition with a role in repairing the keratinous barrier, presumably because the synergistic effect of linolenic acid and unsaturated fatty acids in evening primrose oil, olive oil and macadamia nut oil may affect the stability of the lipid membranes of the epidermal cells and form lacunae to increase the permeability of the skin and increase the absorption of esters by the epidermal cells. However, the stability of the active ingredients in evening primrose oil and olive oil is poor, the active ingredients are easy to be oxidized, and the active ingredients can eliminate oxidation free radicals by introducing the participation of 2-alpha glycerol glucoside, so that the stability of the vegetable oil is improved.

In a preferred embodiment, the peptide compound is selected from one or more of acetyl hexapeptide-3, palmitoyl pentapeptide-3 and acetyl tetrapeptide-5.

In a preferred embodiment, the peptidic compound is palmitoyl pentapeptide-3.

The applicant finds that under the action of palmitoyl pentapeptide-3, collagen in skin fiber cells can be promoted to be combined with the glycerol glucoside, so that the skin compactness is increased, and the generation of skin wrinkles is delayed. And the collagen in the skin is combined with the glycerol glucoside, so that the moisture of skin cells is increased, and the luster and the transparency of the skin are increased by moisturizing and moisturizing.

As a preferred embodiment, the small molecule organic alcohol is selected from one or more of glycerol, 1, 4-butanediol, 1, 6-hexanediol, polyethylene glycol, sorbitol and propylene glycol.

As a preferred embodiment, the small molecule organic alcohol is a combination of glycerol and 1, 4-butanediol.

As a preferred embodiment, the weight ratio of glycerol to 1, 4-butanediol is 1: (0.3-0.8).

As a preferred embodiment, the weight ratio of glycerol to 1, 4-butanediol is 1:0.5.

as a preferred embodiment, the skin conditioning agent is selected from one or more of pectin, sodium hyaluronate, guar gum, carageenan, carrageenan, and acrylate cross-linked polymer.

As a preferred embodiment, the skin conditioning agent is a carrageenan.

The second aspect of the present invention provides a spot-removing whitening product comprising the use of a polysaccharide or a derivative thereof in a spot-removing whitening product, characterized by being prepared using the raw material of claim 4.

As a preferred embodiment, the preparation method of the freckle removing and whitening product comprises the following steps:

(1) Mixing glycerol glucoside, plant extract, vegetable oil composition, small molecular organic alcohol and deionized water, heating to 55-70 deg.C, stirring at 500-800rps for 15-25min;

(2) Cooling to 40-50 deg.C, adding skin conditioner, peptide compound, and 500-550rps, stirring, cooling to room temperature, and sealing for storage.

Compared with the prior art, the invention has the following beneficial effects:

(1) The invention provides an application of polysaccharide and derivatives thereof in freckle removing and whitening products, which specifically adopts 0.5-3% by weight of glycerol glucoside with the three-dimensional configuration of 2-alpha GG to apply in the freckle removing and whitening products, so that the composition can penetrate into the dermis of skin to supplement water for the dermis of the skin, and the freckle removing and whitening products have the long-acting moisturizing effect.

(2) The invention provides application of polysaccharides and derivatives thereof in freckle removing and whitening products, which can enable a composition to have the effect of repairing and protecting skin barriers by combining evening primrose oil, olive oil and macadamia nut oil, and particularly comprises the following components in percentage by weight: 13:22, the composition can promote skin protein expression and reduce skin sensitivity after long-term use.

(3) The invention provides application of polysaccharides and derivatives thereof in freckle removing and whitening products, which can reduce the oxidation activity of the freckle removing and whitening products through the synergistic effect of the combination of plant oil of evening primrose oil, olive oil and macadamia nut oil and glycerol glucoside, thereby improving the stability of the composition.

(4) The polysaccharide and the polysaccharide derivative are specifically used as a combination of plant extracts, and the plant extracts and the glycerol glucoside have synergistic effect, so that the compatibility among all components in the composition can be improved, the composition can be quickly absorbed by skin when in use, and the greasy feeling of the skin is reduced.

(5) The polysaccharide and the derivatives thereof provided by the invention are applied to the freckle removing and whitening product, and particularly, the synergic action of the low-molecular-weight glycerol glucoside, the carrageenin and the palmitoyl pentapeptide-3 is adopted, so that the freckle removing and whitening product can firmly lock skin moisture while deeply penetrating into the skin to replenish the moisture, the moisturizing effect of the freckle removing and whitening product is further improved, the skin can be kept moist for 24 hours, and the skin is prevented from being oily.

(6) According to the application of the polysaccharides and the derivatives thereof in the freckle removing and whitening product, the prepared freckle removing and whitening product can go deep into the deep layer of skin, inhibits the action of melanocyte tyrosinase, achieves the effects of whitening and removing freckles, can inhibit anaphylactic reaction, and has the effect of relieving the skin.

Drawings

FIG. 1 is a fluorescence test chart of water supplement effect of a control group;

FIG. 2 is a fluorescence test chart of the water replenishing effect in example 1;

FIG. 3 is a fluorescence test chart of the water replenishing effect in example 2;

FIG. 4 is a fluorescence test chart showing the water replenishing effect in example 3;

FIG. 5 is a photograph of a negative control cell repair;

FIG. 6 is a photograph of a positive control cell repair;

FIG. 7 is a photograph taken of the cell repair of example 1;

FIG. 8 is a photograph taken in example 2 for repairing cells;

FIG. 9 is a photograph taken of the cell repair of example 3;

FIG. 10 is a photograph taken of the cell repair of example 4;

FIG. 11 is a bar graph of the results of a relative inhibition of cellular melanin;

FIG. 12 is a bar graph showing the results of the test of the inhibition rate of the tyrosinase activity of the cells.

Detailed Description

Example 1

A speckle removing and whitening product prepared by applying polysaccharides and derivatives thereof in the speckle removing and whitening product comprises the following raw materials in percentage by weight: 1% of glycerol glucoside, 7.8% of plant extract, 22% of vegetable oil composition, 1.1% of peptide compound, 10% of small molecular organic alcohol, 13% of skin conditioner and the balance of deionized water.

The spatial configuration of the glycerol glucoside is 2-alpha GG, and is purchased from Chinesemedicine development Co., ltd.

The plant extract is a combination of seaweed extract, aloe extract and centella asiatica extract, and the weight ratio of the seaweed extract to the aloe extract to the centella asiatica extract is 4:2.5:1.3. the seaweed extract was purchased from Illia technologies, inc., guangzhou; the aloe vera extract was obtained from Savino Biotech, inc. and the centella asiatica extract was obtained from Guangzhou Di Pu Biotech, inc.

The vegetable oil composition is a combination of evening primrose oil, olive oil and macadamia nut oil, and the weight ratio is 32:13:22.

the peptide compound is palmitoyl pentapeptide-3, which is purchased from Nanjing Lyon Biotechnology Limited.

The micromolecular organic alcohol is a combination of glycerol and 1, 4-butanediol, and the weight ratio is 1:0.5.

the skin conditioner is a carrageenan.

The preparation method of the freckle removing and whitening product comprises the following steps:

(1) Mixing glycerol glucoside, plant extract, vegetable oil composition, small molecular organic alcohol and deionized water, heating to 65 ℃, stirring at 550rps for 20min;

(2) Cooling to 40 deg.C, adding skin conditioner, peptide compound, and 500rps, stirring, cooling to room temperature, and sealing for storage.

Example 2

The specific steps of the freckle removing and whitening product prepared by applying the polysaccharides and the derivatives thereof in the freckle removing and whitening product are the same as those of the example 1, and are different from that of the example 1 in that the weight percentage of the glycerol glucoside is 2%.

Example 3

The specific steps of the freckle removing and whitening product prepared by applying the polysaccharides and the derivatives thereof in the freckle removing and whitening product are the same as those in example 1, and are different in that the weight percentage of the glycerol glucoside is 3%.

Example 4

The specific steps of the freckle removing and whitening product prepared by applying the polysaccharides and the derivatives thereof in the freckle removing and whitening product are the same as those of the example 1, and are different from that of the example 1 in that the weight percentage of the glycerol glucoside is 0.5%.

Performance test

Improvement of keratinocyte barrier function: the cells used were human skin immortalized keratinocytes, and the barrier genes (expression of TGM1& LOR) were tested.

The experimental method comprises the following steps: (1) cell inoculation: cells in logarithmic growth phase were collected at a cell density of 1X 10 ⁵ Seed/well into 24-well plates, incubate for 24h in incubator (37 ℃,5% CO2); (2) The compositions of examples 1-3 were inoculated, untreated cells served as controls, and 3 replicate wells were set per group; (3) Add 0.5mL of lysis buffer to each well and blow-lyse. Extracting RNA, reverse transcribing to cDNA, and fluorescent quantitative PCR detection

The method performs a result calculation. The calculation results are shown in Table 1.

TABLE 1

Compared with a control group, when the weight ratio of the glycerol glucoside is 3%, the TGM1& LOR genes are not obviously improved, when the weight ratio is 1% and 2%, the TGM1 expression level of cells is obviously improved, and when the weight ratio is 1%, the LOR expression level of the cells is obviously improved, which indicates that the composition can improve the barrier function of keratinocytes at the concentrations of 2% and 1%.

And (3) testing the water replenishing effect: the cells used in the test were human skin immortalized keratinocytes and AQP3 aquaporin expression was assessed.

The experimental method comprises the following steps: (1) cell inoculation: inoculating the cells to a 24-well plate at an inoculation density of 2X 105 cells/well, incubating overnight in an incubator (37 ℃, 5%; CO2); (2) preparation examples 1,2,3; (3) When the cell plating rate in the 24-well plate reaches 40% -60%, performing a grouping test, adding no test sample in a control group, wherein the adding amount of each hole is 1.0mL, each group is provided with 3 repeated holes, and continuously culturing for 24h in an incubator (37 ℃,5% CO2); (4) collecting samples: discarding the supernatant, and rinsing the cells for 3 times with PBS; (5) immunofluorescence staining: a conventional immunofluorescent staining procedure was performed. The method mainly comprises the following steps: fixing, sealing, adding primary antibody, adding secondary antibody and DAPI for nuclear staining, and taking a picture by using a fluorescence microscope; (6) analysis of results: AQP3 fluorescence intensity was quantitatively analyzed using Image Pro Plus software. The results of the fluorescence measurements are shown in FIGS. 1-4, where FIG. 1 is a control, FIG. 2 is example 1, FIG. 3 is example 2, FIG. 4 is example 3, and three sets of parallel experiments are shown. The results of the tests of examples 1-3 against the control are shown in Table 2.

TABLE 2

Compared with a control group, when the concentration of the glycerol glucoside is 2% and 1%, the green fluorescence of the AQP3 protein is remarkably increased, which shows that the expression of the aquaporin AQP3 of the HaCaT cell can be promoted, and the moisturizing effect is realized.

Cell repair assay: the cells used were tested as human skin immortalized keratinocytes and AQP3 aquaporin expression was assessed.

The experimental method comprises the following steps: (1) Cells in the logarithmic growth phase were collected and seeded at a cell density of 2X 105 cells/well in 24-well culture plates. After culturing in an incubator (37 ℃,5% CO2) for 24 hours, "lesions" were scratched in a 200. Mu.l tip in a 24-well plate, the cells were washed 3 times with PBS, the scratched cells were removed, and a fetal bovine serum-free medium was added. Example 1,2,3,4 was added, and the cell group to which PBS was added was used as a positive control and a negative control, and the cells were cultured in an incubator (37 ℃ C., 5% CO2) for 24 hours, each group being set to 3 replicates. Each group of cells was photographed using an inverted microscope and the average value of the scratch area was calculated using Image Pro Plus software. The photographs were taken in the figures 5-10, with figure 5 being the negative control, figure 6 being the positive control, and figures 7-10 being examples 1-4.

Percent healing = (initial scratch area-present scratch area)/initial scratch area × 100%

The test results are shown in Table 3.

TABLE 3

The cell healing level of the positive control group is obviously increased compared with that of the negative control group in the cell repair test (p<0.05 ); compared with a negative control group, the healing rate of the cells is obviously increased after the samples of 0.5 percent and 1 percent (V/V) are treated. The cell healing rate was increased after treatment with 0.5% and 1% (V/V) samples, which had cell repair efficacy, compared to the control group.

Melanin tyrosinase inhibition test: the cells used in the test were mouse melanoma cells and the in vitro whitening efficacy assessment (melanin content inhibition & tyrosinase activity inhibition) was tested.

The experimental method comprises the following steps:

collecting cells in logarithmic growth phase according to the cell density of 2 × 10 ⁵ One/vial was inoculated into 6-well plates, cultured in an incubator (37 ℃ C., 5% CO2) for 24 hours, and then, based on the cytotoxicity results, 3 replicates of each group were set by adding each of examples 1,2,4, using kojic acid at a mass concentration of 0.3% as a positive control group and untreated cells as a blank control group. Adding drug, culturing in incubator (37 deg.C)5% CO2), discarding the supernatant, adding 1mL of 1M NaOH containing 10% DMSO, incubating in a constant temperature oven at 80 ℃ for 30min, reading the absorbance value at 475nm with 1M NaOH containing 10% DMSO as a blank, and calculating the relative inhibition of melanin in the cells. The test results are shown in table 4 and fig. 11.

[1- (addition well OD-blank well OD)/(control well OD-blank well OD) ] × 100% = melanogenesis inhibitory rate%

Cells in the logarithmic growth phase were collected and seeded at a cell density of 1X 104 cells/well in 96-well plates. After 24 hours of incubation in an incubator (37 ℃,5% CO2), 3 replicates of each group were set up, based on the cytotoxicity results, with kojic acid at 0.3% mass concentration as the positive control and untreated cells as the blank, according to example 1,2, 4. Adding medicine, culturing in incubator (37 deg.C, 5% CO2) for 24h, discarding culture medium, washing with PBS for 2 times, adding 100 μ L of 0.5% TritonX-100 into each well, lysing at-80 deg.C for 2h, taking out 96-well plate, naturally thawing at room temperature, adding 50 μ L of 10mmol/L levodopa into each well, incubating at 37 deg.C for 2h, and reading absorbance at 475nm to calculate the inhibition rate of tyrosinase activity. The test results are shown in table 4 and fig. 12.

[1- (administration well OD-blank well OD)/(control well OD-blank well OD) ] × 100% = inhibition of cellular tyrosinase activity% ]

TABLE 4

In the experiment of the melanin synthesis inhibition of cells, the melanin synthesis inhibition rate of the cells treated by 2% (V/V) samples is improved, and the cells have statistical difference (p < 0.05) compared with a blank control and have the capacity of reducing the melanin synthesis of the cells.

In the inhibition test of the cell tyrosinase activity, the inhibition rate of the cell tyrosinase activity treated by 2%, 1% and 0.50% (V/V) samples is obviously increased, and compared with a blank control, the inhibition rate has statistical difference (p is less than 0.05), and the samples have the effect of inhibiting the cell tyrosinase activity.

5. And (3) testing the relieving effect: the cells used for the test were mast cells P815, purchased from the cell bank of the chinese academy of sciences. Mast cells are used as experimental objects, a degranulation model is established by stimulating the mast cells through C48/80, and the relieving efficacy of a sample to be tested is evaluated by combining the form and the degranulation rate.

The experimental method comprises the following steps: (1) cell inoculation: cells were seeded at a seeding density of 1X 105 cells/well in 24-well plates and incubated overnight in an incubator (37 ℃ C., 5% CO2). (2) liquid preparation: examples 1,2,4; (3) administration: grouping according to experiments in table 3, when the cell plating rate in a 24-well plate reaches 40% -50%, performing grouped drug delivery, wherein each group is provided with 3 multiple wells, and each well is added with 1mL of culture medium containing samples to be detected with different concentrations. After completion of the administration, the 24-well plate was incubated in an incubator (37 ℃ C., 5% CO2) for 2 hours. (4) C48/80 stimulation: after incubating the sample for 2h, discarding the solution; according to experimental grouping, 1mL of serum-free high-sugar DMEM culture solution is added into a blank control group, 1mL of serum-free high-sugar DMEM culture solution containing C48/80 is added into a negative control group, 1mL of serum-free high-sugar DMEM culture solution containing C48/80 and cromolyn sodium is added into a positive control group, 1mL of serum-free high-sugar DMEM culture solution containing C48/80 and corresponding sample administration concentration is added into a sample group, C48/80 stimulation is carried out for 45 min after the administration is finished, and the reaction is stopped in an ice bath. (5) cell morphology observation: after the culture is finished, the degranulation of each group of cells is observed under an inverted microscope and photographed. The photographs were counted and the degranulation rate of the cells was calculated using IPP software. The test results are shown in Table 5.

TABLE 5

Based on mast cells, the composition shows obvious improvement effect on mast cell degranulation phenomenon at the concentration of 1% and 2%, and has good relieving effect on skin.

6. Human body efficacy skin melanin content testing: 33 healthy Chinese male or female subjects were selected, with the age range of 25-60 years, according to the ASIAN TYPE VOLUME 2 classification from SKIN AGEING ATLAS 1 to 6. The skin melanin content of the subjects was measured and partial and full face photographs were taken, using the test product, continuously for 2 weeks, 4 weeks, respectively, before using the product. The evaluation results before and after the product is used are compared by a statistical test method to judge whether the product has statistical difference.

The test method comprises the following steps: clean face and wait: the subject cleans the face with the face cleaning product, dries with dry facial tissue, sits still for 20min in a laboratory at a temperature of 21 ℃ and a humidity of 50%; the subjects' cheeks were tested by the laboratory using the skin melanin content and hemoglobin tester, mexameter MX18, 2 times daily, morning and evening, using the product of example 1, after 2 weeks and 4 weeks, respectively, according to the method described above. The test results are shown in Table 6.

TABLE 6

The skin melanin content of the product using area of the subject is remarkably reduced (p is less than 0.001) compared with a basic value in 2 weeks of using the product, and the reduction rate is 13.84%; compared with the basic value, the reduction rate is 20.49 percent when the product is used for 4 weeks, and the p is less than 0.001.

Claims

1. The application of polysaccharides and derivatives thereof in freckle removing and whitening products is characterized in that the content of the polysaccharides and the derivatives thereof in the freckle removing and whitening products is 0.01% -10%; the application of the freckle removing and whitening product is selected from one of skin lotion, skin cream, essence, ampoule, facial mask, secondary polishing and eye cream.

2. The polysaccharide and the polysaccharide derivative are used in the freckle removing and whitening product according to claim 1, wherein the polysaccharide and the polysaccharide derivative are at least one of starch, cellulose, glycogen and glucoside.

3. The polysaccharide and the polysaccharide derivatives are applied to the freckle removing and whitening product according to claim 2, wherein the glucoside is glycerol glucoside; the spatial configuration of the glycerol glucoside is any one of 2-alpha GG and 2-beta GG.

4. The polysaccharide and the polysaccharide derivatives are applied to the freckle removing and whitening product according to claim 3, wherein the glycerol glucoside is extracted from cyanobacteria cells.

5. The polysaccharide and the polysaccharide derivatives as claimed in claim 4, wherein the product for removing freckles and whitening skin comprises one of whitening skin, removing freckles, repairing barriers, resisting aging, diminishing inflammation, moisturizing skin, and refining pores to remove freckles and whiten skin.

6. The polysaccharide and the polysaccharide derivatives are applied to the freckle removing and whitening product according to claim 5, wherein the weight percentage of the glycerol glucoside in the freckle removing and whitening product is 0.5% -3%; the spatial configuration of the glycerol glucoside is 2-alpha GG.

7. The polysaccharide and the polysaccharide derivatives in the freckle removing and whitening product according to claim 5 or 6 are characterized in that the freckle removing and whitening product is prepared from the following raw materials in percentage by weight: 0.5-3% of glycerol glucoside, 1-15% of plant extract, 10-30% of vegetable oil composition, 0.1-5% of peptide compound, 10-40% of small molecular organic alcohol, 10-20% of skin conditioner and the balance of deionized water.

8. The polysaccharide and the polysaccharide derivatives are used in the freckle removing and whitening product according to claim 7, wherein the plant extract is one or more of seaweed extract, aloe extract, centella asiatica extract, salvia miltiorrhiza extract, grapefruit extract, hawthorn extract, chamomile extract and witch hazel extract.

9. The polysaccharide and the polysaccharide derivatives are used in the spot removing and whitening product according to claim 7, wherein the vegetable oil composition is selected from one or more of evening primrose oil, olive oil, macadamia nut oil, borage oil, castor oil, jojoba seed oil, shea butter, camellia oil and sunflower seed oil.

10. A speckle removing and whitening product comprising the polysaccharide or polysaccharide derivative according to any one of claims 1 to 9 for use in a speckle removing and whitening product, which is prepared using the raw material according to claim 7.