CN115219421A

CN115219421A - Microfluidic detection device and method

Info

Publication number: CN115219421A
Application number: CN202210701185.3A
Authority: CN
Inventors: 宋金召; 庞飞彪; 代丰翼; 王开正; 骆继豪; 张昊; 张礼懿
Original assignee: Hangzhou Yijian Technology Co ltd
Current assignee: Hangzhou Yijian Technology Co ltd
Priority date: 2022-06-20
Filing date: 2022-06-20
Publication date: 2022-10-21

Abstract

The device comprises a main body, wherein a chip loading disc is arranged on the main body and used for connecting a micro-fluidic chip, and the micro-fluidic chip is used for accommodating an object to be detected and a detection agent; the main body is provided with a rotating module, and the rotating module is connected with the chip loading disc and is used for controlling the chip loading disc to rotate so as to drive the microfluidic chip to rotate, so that an object to be detected is mixed with a detection agent to generate a detection reaction; the main body is also provided with a temperature control module which forms a temperature control cavity for accommodating the microfluidic chip; the main body is provided with a control module, the control module is in communication connection with the rotating module, the temperature module and the mobile terminal device, and the control module is used for receiving a reaction instruction from the mobile terminal device so as to control the rotating module and the temperature control module according to the reaction instruction; the main body is provided with an observation module, the observation module is positioned in the appointed direction of the microfluidic chip, and the observation module is used for observing the detection reaction of the object to be detected and the detection agent.

Description

Microfluidic detection device and method

Technical Field

The disclosure relates to the technical field of microfluidics, in particular to a microfluidic detection device and a microfluidic detection method.

Background

The micro-fluidic chip is based on precise micro-channel design and standardized program control of a matched instrument, has strong controllability and high stability, and is not influenced by factors such as artificial errors; the microfluidic chip has high integration level, and can integrate complex steps such as sample processing, amplification, detection and the like into the chip and matched equipment; the detection device based on the microfluidic chip can realize nucleic acid detection so as to enable a user to complete self-detection, but the conventional common microfluidic detection system is complex in control, complex in structure and overlarge in volume, so that the portability is poor and the operation is inconvenient.

Disclosure of Invention

The present disclosure provides a microfluidic detection device and method to at least solve the above technical problems in the prior art.

According to a first aspect of the present disclosure, there is provided a microfluidic detection device, the device comprising a main body, a chip loading disc is arranged on the main body, the chip loading disc is used for connecting a microfluidic chip, and the microfluidic chip is used for accommodating an object to be detected and a detection agent; the main body is provided with a rotating module, the rotating module is connected with the chip loading disc, and the rotating module is used for controlling the chip loading disc to rotate so as to drive the micro-fluidic chip to rotate, so that an object to be detected is mixed with the detection agent to generate a detection reaction; the main body is provided with a control module, the control module is in communication connection with the rotating module, the control module is in communication connection with a mobile terminal device, the control module is used for receiving a reaction instruction from the mobile terminal device, and the control module is used for controlling the rotating module according to the reaction instruction; the main body is provided with an observation module, the observation module is positioned in the appointed direction of the microfluidic chip, and the observation module is used for observing the detection reaction of the object to be detected and the detection agent.

In an implementation manner, a temperature control module is further disposed on the main body, and a temperature control cavity is formed in the temperature control module and used for accommodating the microfluidic chip; the temperature control module is in communication connection with the control module, and the control module is used for controlling the temperature of the temperature control cavity.

In one embodiment, the temperature control module comprises a cover plate, a bottom plate and a heating element; the cover plate and the bottom plate enclose to form the heating cavity; the heating element is connected with the cover plate and used for heating the cover plate so as to heat the temperature control cavity; and/or the heating element is connected with the bottom plate, and the heating element is used for heating the bottom plate so as to heat the temperature control cavity.

In an implementation manner, the rotating module includes a driving member, a transmission member and a mounting plate, the driving member is connected to the main body through the mounting plate, the driving member is connected to the chip loading tray through the transmission member, and the driving member drives the transmission member to rotate so as to drive the chip loading tray to rotate.

In one embodiment, the chip loading tray includes a chip tray, a slider, a limiter, and a tray base; a sliding groove is formed in the first surface of the chip tray and used for the sliding connection of the sliding block; a limiting groove is formed on the second surface of the chip tray and used for connecting the limiting piece; the limiting piece comprises a connecting part and a limiting part, the groove is communicated with the sliding groove, when the limiting piece is positioned in the limiting groove, the connecting part is positioned in the limiting groove, the limiting part is positioned in the sliding groove, and the limiting part is used for abutting against the sliding block; the tray base is connected to the second surface of the chip tray and used for fastening the limiting piece in the limiting groove; wherein the first surface and the second surface are in different positions; the sliding block is provided with a first inclined plane, and an elastic part is arranged between the sliding block and the chip loading disc; under the condition that the mounting hole of the microfluidic chip slides along the first inclined plane, the sliding block slides towards the first direction under the action of the microfluidic chip, so that the mounting hole penetrates through the sliding block and is abutted to the chip tray; when the microfluidic chip is abutted against the chip tray, the sliding block is abutted against the limiting part under the action of the elastic part, and the sliding block is used for fastening the microfluidic chip on the chip tray.

In an embodiment, the slider is formed with a second inclined surface, and the direction of the second inclined surface is different from that of the first inclined surface; under the condition that the microfluidic chip slides along the second inclined surface, the sliding block slides towards a second direction under the action of the microfluidic chip, so that the mounting hole penetrates through the sliding block and is separated from the chip tray; wherein the first direction and the second direction are different.

In one embodiment, the chip loading tray is formed with a connection hole for the connection of the transmission member; when the transmission piece is positioned in the connecting hole, the transmission piece is fastened in the connecting hole through a fastening piece; wherein, the driving medium includes D-shaped axle at least.

In one embodiment, the main body comprises an upper cover and a box body; the upper cover is connected with the box body through a hinge piece, so that the upper cover can rotate relative to the box body along the hinge piece.

According to a second aspect of the present disclosure, there is provided a microfluidic chip detection method, the method comprising: installing the micro-fluidic chip containing the object to be detected and the detection agent on a chip loading disc of the micro-fluidic detection device; a control module of the microfluidic detection device receives a reaction instruction from the mobile terminal equipment; the control module controls the rotating module to drive the chip loading disc to rotate according to the reaction instruction so as to drive the micro-fluidic chip to rotate, so that the object to be detected and the detection agent are mixed to generate a detection reaction; and under the condition that the object to be detected and the detection agent are mixed to generate a detection reaction, the control module controls the observation module to observe the microfluidic chip from the specified direction so as to obtain a detection result.

In an embodiment, after the control module of the microfluidic detection apparatus receives the reaction instruction from the mobile terminal device, the method further includes: the control module controls the temperature control module to adjust the temperature in the temperature control cavity according to the reaction instruction to obtain the temperature control cavity with the specified temperature; the control module controls the rotating module to drive the chip loading disc to rotate according to the reaction instruction so as to drive the micro-fluidic chip to rotate, so that the object to be detected and the detection agent are mixed in the temperature control cavity at the specified temperature to generate a detection reaction.

The microfluidic detection device and the method of the present disclosure are characterized in that a microfluidic chip containing an object to be detected and a detection agent is mounted on a chip loading disc of the microfluidic detection device; the mobile terminal equipment is in communication connection with the control module to control the rotating module, so that the rotating module drives the chip loading disc to rotate to drive the micro-fluidic chip to rotate, and the object to be detected is mixed with the detection agent to generate a detection reaction; and observing the microfluidic chip from the specified direction through an observation module to obtain a detection result. Through all integrating control module, rotation module and observation module in the main part, cooperation mobile terminal's control conveniently carries easy operation.

It should be understood that the statements in this section do not necessarily identify key or critical features of the embodiments of the present disclosure, nor do they limit the scope of the present disclosure. Other features of the present disclosure will become apparent from the following description.

Drawings

The above and other objects, features and advantages of exemplary embodiments of the present disclosure will become readily apparent from the following detailed description, which proceeds with reference to the accompanying drawings. Several embodiments of the present disclosure are illustrated by way of example, and not by way of limitation, in the figures of the accompanying drawings and in which:

in the drawings, the same or corresponding reference numerals indicate the same or corresponding parts.

Fig. 1 shows a schematic structural diagram of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 2 shows a schematic structural diagram ii of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 3 shows a schematic structural diagram three of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 4 shows a schematic structural diagram of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 5 shows a schematic structural diagram of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 6 shows a schematic structural diagram six of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 7 shows a schematic structural diagram seven of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 8 shows a schematic structural diagram eight of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 9 shows a schematic structural diagram nine of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 10 shows a schematic structural diagram of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 11 shows an eleventh schematic structural diagram of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 12 shows a schematic structural diagram twelve of a microfluidic detection device according to an embodiment of the present disclosure;

fig. 13 shows a schematic structural diagram thirteen of a microfluidic detection device according to an embodiment of the present disclosure.

The reference numbers in the figures illustrate:

1. a main body; 11. an articulation member; 12. an upper cover; 13. a box body; 2. a rotation module; 21. a drive member; 22. a transmission member; 23. mounting a plate; 211. a driving member supporting column; 221. a D-shaped shaft; 222. a threaded hole; 3. a chip loading tray; 31. a chip tray; 32. a slider; 33. a limiting member; 34. a tray base; 35. connecting holes; 311. a chute; 313. a first surface; 314. a second surface; 321. a first inclined plane; 322. a second inclined plane; 331. a connecting portion; 332. a limiting part; 4. a control module; 5. an observation module; 51. an imaging unit; 52. an LED lamp; 53. detecting a camera; 54. a camera drive board; 55. a camera mounting hole; 56. an optical filter; 57. transparent glass; 6. a temperature control module; 61. a temperature control cavity; 62. a cover plate; 63. a base plate; 64. a heating element; 621. a cover plate mounting plate; 631. a bottom plate mounting hole; 632. a support pillar; 7. a microfluidic chip; 8. a mobile terminal device; 91. a base; 101. a diluent chamber; 102. a sample chamber; 103. a mixing chamber; 104. a liquid separation cavity; 105. a reaction chamber; 106. a waste fluid chamber; 107. a gas circulation path; 108. a capillary valve; 109. a siphon valve.

Detailed Description

In order to make the objects, features and advantages of the present disclosure more apparent and understandable, the technical solutions in the embodiments of the present disclosure will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present disclosure, and it is apparent that the described embodiments are only a part of the embodiments of the present disclosure, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments disclosed herein without making any creative effort, shall fall within the protection scope of the present disclosure.

Fig. 1 shows a schematic structural diagram of a microfluidic detection device according to an embodiment of the present disclosure; fig. 2 shows a schematic structural diagram of a microfluidic detection device according to an embodiment of the present disclosure; fig. 3 shows a schematic structural diagram three of a microfluidic detection device according to an embodiment of the present disclosure; fig. 4 shows a schematic structural diagram of a microfluidic detection device according to an embodiment of the present disclosure; fig. 5 shows a schematic structural diagram of a microfluidic detection device according to an embodiment of the present disclosure; please refer to fig. 1-5;

according to a first aspect of the disclosure, a microfluidic detection device is provided, the device comprises a main body 1, a chip loading disc 3 is arranged on the main body 1, the chip loading disc 3 is used for connecting a microfluidic chip 7, and the microfluidic chip 7 is used for accommodating an object to be detected and a detection agent; the main body 1 is provided with a rotating module 2, the rotating module 2 is connected with the chip loading disc 3, and the rotating module 2 is used for controlling the chip loading disc 3 to rotate so as to drive the microfluidic chip 7 to rotate, so that an object to be detected is mixed with a detection agent to generate a detection reaction; the main body 1 is provided with a control module 4, the control module 4 is in communication connection with the rotating module 2, the control module 4 is in communication connection with a mobile terminal device 8, the control module 4 is used for receiving a reaction instruction from the mobile terminal device 8, and the control module 4 is used for controlling the rotating module 2 according to the reaction instruction; the main body 1 is provided with an observation module 5, the observation module 5 is positioned in the appointed direction of the microfluidic chip 7, and the observation module 5 is used for observing the detection reaction of an object to be detected and a detection agent.

According to the microfluidic detection device and the method, the microfluidic chip 7 containing the object to be detected and the detection agent is arranged on the chip loading disc 3 of the microfluidic detection device; the mobile terminal device 8 is in communication connection with the control module 4 to control the rotating module, so that the rotating module drives the chip loading disc 3 to rotate to drive the micro-fluidic chip 7 to rotate, and the object to be detected is mixed with the detection agent to generate a detection reaction; and observing the microfluidic chip 7 from the specified direction through the observation module 5 to obtain a detection result. Through all integrating control module 4, rotation module and observation module 5 in main part 1, cooperation mobile terminal 8's control conveniently carries easy operation.

In the disclosed embodiment, the device comprises a main body 1, the shape of the main body 1 and a box body 13, so as to be convenient to carry and transport; specifically, the main body 1 includes an upper cover 12 and a box body 13; the upper cover 12 is connected with the box body 13 through the hinge 11, so that the upper cover 12 can rotate relative to the box body 13 along the hinge 11, the upper cover 12 can be opened and closed, and operations such as installation and disassembly of the microfluidic chip 7 can be carried out under the condition that the upper cover 12 is opened; when the upper lid 12 is closed, the detection reaction of the microfluidic chip 7, observation of the detection reaction, and the like are performed. The main body 1 is provided with a chip loading tray 3, and the chip loading tray 3 is used for mounting the microfluidic chip 7, wherein the chip loading tray 3 is arranged in a box body 13 of the main body 1. The micro-fluidic chip 7 is used for accommodating an object to be detected and a detection agent, and the micro-fluidic chip 7 is a centrifugal micro-fluidic chip 7; wherein, the object to be detected and the detection agent are positioned at different positions of the microfluidic chip 7, and the object to be detected and the detection agent are required to be mixed under the action of centrifugal force so as to carry out detection reaction. Therefore, the main body 1 is provided with the rotating module 2, the rotating module 2 is arranged in the box body 13 of the main body 1 and specifically positioned below the chip loading disc 3, and the rotating module 2 is used for controlling the chip loading disc 3 to rotate so as to drive the microfluidic chip 7 to rotate, so that the object to be detected is mixed with the detection agent to generate the detection reaction. The rotating speed of the rotating module 2 is controlled by the control module 4, the control module 4 is in communication connection with the mobile terminal device 8, the control mechanism can be arranged in the control box, the rotating speed required by the rotating module 2 is determined through the reaction instruction by receiving the reaction instruction from the mobile terminal device 8, so that the micro-fluidic chip 7 is controlled to rotate at the specified rotating speed, and the object to be detected and the detection agent can be completely mixed. And controlling the object to be detected to enter different cavities of the microfluidic chip 7 through different rotating speeds, thereby realizing the control of the detection flow of the object to be detected. The mobile terminal device 8 may be a mobile phone, a tablet, a notebook computer, or the like, so as to implement rapid control, data acquisition, and the like. The control module 4 is used for controlling the rotating module 2 according to the reaction instruction; the main body 1 is provided with an observation module 5, and when an object to be detected and a detection agent are subjected to detection reaction, the detection reaction is observed through the observation module 5, so that reaction data are obtained, and the reaction data are sent to the mobile terminal device 8 through the control module 4, so that a detection result is obtained. Because the micro-fluidic chip 7 is internally gas-circulated, the micro-fluidic chip is not polluted by the outside and cannot pollute the outside. Combine the micro-fluidic detection device who integrates, the cooperation removes end equipment, can be convenient carry out user's self-checking operation to and obtain the testing result fast.

The control module 4 is used for collecting data by controlling the observation module 5 and sending the data to the mobile terminal device 8, and the mobile terminal device 8 is used for processing the received data and displaying a detection result; as shown in fig. 3, the observation module 5 is disposed above the upper cover 12, wherein a transparent glass 57, which may be a ring-shaped glass, may be disposed on the periphery of the cover plate 62, and a circular hole for the cover plate 62 to be inserted into is formed in the middle of the cover plate 62, so as to ensure the tightness of the device; furthermore, the structure of the ring-shaped glass can be matched with the detection principle of the microfluidic chip, so that the reaction chambers distributed annularly on the microfluidic chip can be accurately observed, and the arrangement of the temperature control module 6 cannot be interfered; the observation module 5 can be an imaging detection module or a photodiode detection module; as shown in fig. 4, the observation module 5 is composed of: the detection module can realize detection of fluorescence signals and detection of colorimetric signals and chemiluminescence; further, a plurality of blue LED lamps 52 may be integrated on the upper cover 12 of the main body 1 as an excitation light source, the LED lamps 52 may be annularly disposed at a position of the upper cover 12 facing the chip loading tray 3, and the irradiated reaction liquid may emit green fluorescence, thereby realizing observation of detection reaction, wherein the wavelength of the LED of the excitation light source is 475nm to 485nm, thereby being capable of realizing fluorescence detection. Wherein the imaging unit 51 is disposed toward the chip loading tray 3 so as to observe the microfluidic chip 7; specifically, the device can comprise a detection camera 53, a camera driving board 54 connected with the detection camera, and a camera mounting hole 55 for mounting the camera; the detection camera 53 and the camera drive board 54 are fixed on the main body 1 through the camera mounting hole 55, and are connected with the control module 4 through the camera drive board 54 to receive a control command from the control module 4, so that the detection camera 54 is driven to detect the microfluidic chip 7. The other structure of the photodiode detection module comprises: the device comprises an excitation light source, an optical fiber and a photodiode array, wherein the excitation light source generates excitation light, the optical fiber realizes the transmission of a fluorescence signal, and the photodiode array realizes the detection of the fluorescence signal. Both of which enable the observation of the detection reaction. In addition, as shown in fig. 5, the observation module 5 may also be a visual observation module, an optical filter window is disposed through the observation module 5, and a user observes the microfluidic chip 7 through the optical filter window, so as to determine a reaction result. Specifically, qualitative analysis can be performed by directly observing the existence of fluorescence of the reaction solution, specifically, observation can be performed under the condition that the wavelength is 475-485nm and a blue LED is used as an excitation light source; alternatively, other reactions, such as colorimetric reactions, may be performed on the chip, and qualitative analysis of the experiment may be performed by observing a change in color of the reaction solution. Specifically, under the condition that the observation module 5 is set as a visual observation module, the micro-fluidic chip 7 can be photographed by the visual observation module through the photographing function in the mobile terminal device 8, so as to obtain an image after reaction, and the image after reaction is analyzed by the mobile terminal device 8, so as to obtain a detection result. Therefore, the user can quickly obtain the detection result without learning related knowledge, the image after reaction can be obtained by photographing, the method is quick and convenient, and the method can be suitable for the working condition that self-detection is carried out on various crowds. The observation module 5 may be provided with an optical filter 56 to cooperate with the imaging module 51 to detect the microfluidic chip 7, or cooperate with the photographing function in the mobile terminal device 8 to detect the microfluidic chip 7. Meanwhile, the detection result can be stored in electronic data through the mobile terminal device 8, and later retrieval, consultation, big data analysis and the like can be better performed.

It should be noted that the microfluidic chip 7 can realize accurate and effective detection in a non-laboratory environment, and particularly has a wide application prospect and application value in the field of point-of-care diagnostics (POCT), and the microfluidic technology has a series of analysis fields such as biochemical index analysis, DNA analysis, immunoassay, toxicity detection and the like. Among them, nucleic acid detection is only one of the applications.

Fig. 6 shows a schematic structural diagram six of a microfluidic detection device according to an embodiment of the present disclosure; fig. 7 shows a schematic structural diagram seven of a microfluidic detection device according to an embodiment of the present disclosure; please refer to fig. 1-7;

in an implementation manner, the main body 1 is further provided with a temperature control module 6, the temperature control module 6 is formed with a temperature control cavity 61, and the temperature control cavity 61 is used for accommodating the microfluidic chip 7; temperature control module 6 and control module 4 communication connection, control module 4 is used for controlling the temperature in temperature control chamber 61.

In the embodiment of the disclosure, a temperature control module 6 is arranged in the main body 1, the temperature control module 6 is formed with a temperature control cavity 61, and the temperature control cavity 61 is used for accommodating the microfluidic chip 7; specifically, the temperature control module 6 is disposed in the case 13 of the main body 1 above the rotation module, and specifically, the chip loading tray 3 is also disposed in the temperature control chamber 61. The temperature control module 6 is in communication connection with the control module 4, and the control module 4 is used for controlling the temperature of the temperature control cavity 61 to obtain the temperature control cavity 61 with the specified temperature; so that the substance to be detected and the detection agent are mixed in the temperature control chamber 61 at a specified temperature to perform a detection reaction. The temperature control module 6 includes a cover plate 62, a base plate 63, and a heating member 64; the cover plate 62 and the bottom plate 63 enclose to form a heating cavity; the heating element 64 is connected with the cover plate 62, and the heating element 64 is used for heating the cover plate 62 so as to heat the temperature control cavity 61; and/or, a heating member 64 is connected to the base plate 63, and the heating member 64 is used for heating the base plate 63 to heat the temperature controlled chamber 61. Wherein, the cover plate 62 is arranged in the upper cover 12 of the main body 1, the bottom plate 63 is arranged in the box body 13 of the main body 1, and after the upper cover 12 of the main body 1 and the box body 13 are covered, the temperature control cavity 61 is formed between the cover plate 62 and the bottom plate 63 in an enclosing manner. The heating element 64 can be a heating plate, heat generated by the heating plate can be transferred to the cover plate 62 and/or the bottom plate 63, and the cover plate 62 and the bottom plate 63 can uniformly transfer the heat generated by the heating plate to the temperature control cavity 61, so that non-contact heating of the temperature control cavity 61 is realized. Further, a temperature sensor is arranged in the temperature control cavity 61, and the temperature sensor measures the temperature of the temperature control cavity 61 and feeds the temperature back to the mobile terminal device 8 to form closed-loop stable temperature control. So that the temperature is maintained at a prescribed temperature to allow the detection reaction to be more complete. Since the space of the temperature control chamber 61 is small, it is preferable that the cover plate 62 and the base plate 63 are heated together and the heating temperature is uniform. The cover plate 62 and the bottom plate 63 are made of materials with good heat-conducting property, such as aluminum plates or silver plates, wherein the bottom plate 63 is provided with a bottom plate mounting hole 631, the bottom plate 63 is fixed by matching with a fastener, and the bottom plate 63 is connected to the mounting plate 23, so that the structure is more stable and firmer.

Fig. 8 shows a schematic structural diagram eight of a microfluidic detection device according to an embodiment of the present disclosure; please refer to fig. 1-8

In one embodiment, the rotation module 2 includes a driving member 21, a transmission member 22 and a mounting plate 23, the driving member 21 is connected to the main body 1 through the mounting plate 23, the driving member 21 is connected to the chip loading tray 3 through the transmission member 22, and the driving member 21 drives the transmission member 22 to rotate so as to drive the chip loading tray 3 to rotate.

In the embodiment of the present disclosure, the rotating module 2 includes a driving member 21, and the driving member 21 may be a motor, specifically, a dc motor. The driving member 21 is located in the box body 13, and can be located above the control module 4 and below the chip loading tray 3; the driving piece 21 is connected with the main body 1 through the mounting plate 23, the mounting plate 23 can be connected with the box body 13 through threads, and a bottom plate 63 of the temperature control module 6 is embedded in the middle of the mounting plate 23; specifically, a motor sealing element can be arranged in the main body 1, a sealing cavity is formed by matching with the mounting plate 23, and the sealing ring is used for accommodating a motor. The driving member 21 is connected to the chip loading tray 3 through the transmission member 22, and the driving member 21 drives the transmission member 22 to rotate so as to drive the chip loading tray 3 to rotate. Wherein, the transmission member 22 is a D-shaped shaft 221, one end of the D-shaped shaft 221 is fixed with the motor, and the other end is connected with the chip loading disc 3, and the chip loading disc 3 can be better driven to rotate through the D-shaped arrangement. Furthermore, the acceleration and deceleration rotation of the microfluidic chip 7 can be realized through the rotating module 2 of the device, the liquid can regularly move in the microfluidic chip 7 by utilizing the Euler force generated in the acceleration process, the direction of the Euler force is determined by the positive and negative of the acceleration, and the liquid flow in the mixing cavity is determined by the Euler force. The base 91 is also provided with a driving piece support column 211, and the driving piece support column 211 is used for connecting the driving piece 21 so as to connect the driving piece 21 to the base 91, so that the driving piece 21 is more stable, excessive shaking cannot occur in the rotating process, and the practicability of the equipment is improved; further, base 91 is connected with mounting panel 23 through support column 632, realizes the fixed of mounting panel 23 to and the fastening of box body 13 between mounting panel 23 and base 91, so that device stable in structure, each spare part disconnect-type design makes things convenient for the injection moulding of part, and quick replacement when damaging.

Fig. 9 shows a schematic structural diagram nine of a microfluidic detection device according to an embodiment of the present disclosure; fig. 10 shows a schematic structural diagram of a microfluidic detection device according to an embodiment of the present disclosure; please refer to fig. 1-10;

in one embodiment, the chip loading tray 3 includes a chip tray 31, a slider 32, a stopper 33, and a tray base 34; the first surface 313 of the chip tray 31 is formed with a sliding slot 311, and the sliding slot 311 is used for the sliding connection of the sliding block 32; the second surface 314 of the chip tray 31 is formed with a limiting groove for connecting the limiting member 33; the limiting member 33 comprises a connecting portion 331 and a limiting portion 332, the groove is communicated with the sliding groove 311, when the limiting member 33 is located in the limiting groove, the connecting portion 331 is located in the limiting groove, the limiting portion 332 is located in the sliding groove 311, and the limiting portion 332 is used for abutting against the sliding block 32; a tray base is attached to the second surface 314 of the chip tray 31, and the tray base 34 is used for fastening the stoppers 33 in the stopper grooves; wherein the first surface 313 and the second surface 314 are located differently; the slider 32 includes a first slope 321, and an elastic member is provided between the slider 32 and the chip loading tray 3; under the condition that the mounting hole of the microfluidic chip 7 slides along the first inclined plane 321, the sliding block 32 slides towards the first direction under the action of the microfluidic chip 7, so that the mounting hole penetrates through the sliding block 32 and abuts against the chip tray 31; when the microfluidic chip 7 abuts against the chip tray 31, the sliding block 32 abuts against the limiting member 33 under the action of the elastic member, and the sliding block 32 is used for fastening the microfluidic chip 7 on the chip tray 31.

In the embodiment of the present disclosure, the chip tray 31 may have a shape of a key slot, and the mounting holes of the microfluidic chip 7 adapted to the chip tray may also have the same shape, so that the design has an advantage of better transmitting the force in the circumferential direction, so that the microfluidic chip 7 can more easily reach the designated rotation speed. The first surface 313 of the chip tray 31 is formed with a sliding slot 311, the sliding slot 311 is used for sliding connection of the sliding block 32, the sliding block 32 can slide in the sliding slot 311, wherein the sliding slot 311 can be in a convex shape, so that the sliding block 32 can be prevented from shifting upwards or downwards in the sliding process; wherein, the first surface 313 refers to a surface of the chip tray 31 for connecting with the microfluidic chip 7, and further, the surface of the chip tray 31 facing the upper cover 12 when being installed in the main body 1, thereby facilitating the installation or removal of the microfluidic chip 7. A second surface 314 of the chip tray 31 is formed with a limiting groove, the second surface 314 refers to a surface of the chip tray 31 facing away from the microfluidic chip 7, and specifically, the first surface 313 and the second surface 314 may be opposite; the limiting groove is used for connecting the limiting part 33; the limiting member 33 may be a limiting clamping seat, the limiting member 33 includes a connecting portion 331 and a limiting portion 332, when the limiting member 33 is inserted into the limiting groove, the connecting portion 331 is located in the limiting groove, and the limiting portion 332 is located inside the sliding groove 311. A through hole is formed between the limiting groove and the sliding groove 311, and the limiting part 332 penetrates through the through hole and then is positioned in the sliding groove 311; the limiting part 332 is used for abutting against the slide block 32; thereby preventing the slider 32 from coming out of the groove; preferably, when the sliding block 32 abuts against the limiting member 33, the sliding block 32 is used for abutting against the microfluidic chip 7 to prevent the microfluidic chip 7 from separating from the chip tray 31; when the slide block 32 abuts against the inner end of the slide slot 311, the slide block 32 can be passed through the mounting hole of the microfluidic chip 7. The tray chassis is fixed on the second surface 314 of the chip tray 31, specifically, when the chip loading tray 3 is mounted, the slider 32, the elastic member and the main body 1 are partially compressed at a designated position, then the limit stopper is inserted into the limit groove, the mounting of the single slider 32 can be completed by loosening the slider 32, another slider 32 is similarly mounted, and finally the bottom plate 63 is mounted on the second surface 314 of the chip tray 31, so that the assembly of the chip loading tray 3 is completed. The tray base 34 is used for fastening the limiting piece 33 in the limiting groove and preventing the limiting piece 33 from falling off.

The slider 32 includes a first slope 321, the first slope 321 being a slope facing away from the first surface 313; an elastic member is provided between the slider 32 and the chip loading tray 3; the slider 32 abuts against the stopper 33 by the elastic member without the external force. Thereby realizing the fixation and the limit of the micro-fluidic chip 7; when the worker mounts the microfluidic chip 7, the mounting hole of the microfluidic chip 7 slides along the first inclined surface 321 toward the first surface 313, the slider 32 slides toward a first direction under the action of the microfluidic chip 7, the first direction refers to a direction in which the slider 32 faces the chute 311, and after the slider 32 slides to a specified position, the mounting hole of the microfluidic chip 7 passes through the slider 32 and abuts against the chip tray 31; when the microfluidic chip 7 abuts against the chip tray 31, the sliding block 32 abuts against the limiting member 33 under the action of the elastic member, and the sliding block 32 is used for fastening the microfluidic chip 7 on the chip tray 31 to prevent the microfluidic chip 7 from being separated from the chip tray 31. Thereby completing the mounting of the microfluidic chip 7. The slide block 32 comprises a second inclined surface 322, the second inclined surface 322 refers to an inclined surface facing the first surface 313, and when the microfluidic chip 7 is detached, the mounting hole slides along the second inclined surface 322 after abutting against the second inclined surface 322 by controlling the microfluidic chip 7 to move away from the chip tray 31; so that the mounting hole passes through the slider 32 to be separated from the chip tray 31; the disassembly of the chip is realized; simple and convenient operation and safety.

In an embodiment, the chip loading tray 3 is formed with a connection hole 35, and the connection hole 35 is used for connecting the transmission member 22; when the transmission member 22 is located in the connection hole 35, the transmission member 22 is fastened in the connection hole 35 by a fastening member; wherein the transmission member 22 at least comprises a D-shaped shaft 221.

In the embodiment of the present disclosure, the chip loading tray 3 is formed with a connection hole 35, and the shape of the connection hole 35 may be the same as the shape of the transmission member 22, specifically, when the transmission member 22 is the D-shaped shaft 221, the connection hole 35 is a D-shaped hole; the transmission element 22 may also be star-shaped or the like; the shaft end of the D-shaped shaft 221 is provided with a threaded hole 222, and a step is designed through the connection hole 35 of the chip loading tray 3, so that after the D-shaped shaft 221 abuts against the connection hole 35, the connection and the positioning of the chip loading tray 3 are realized through the matching of the threads and the threaded hole 222.

In one embodiment, the main body 1 comprises an upper cover 12 and a box 13; the upper cover 12 is connected to the case 13 by a hinge 11 so that the upper cover 12 can rotate relative to the case 13 along the hinge 11.

In the disclosed embodiment, the main body 1 includes an upper cover 12 and a case 13; the upper cover 12 is connected to the box 13 by a hinge 11 so that the upper cover 12 can rotate along the hinge 11 with respect to the box 13. Thereby realizing the opening and closing operations. In addition, a power switch is connected to the main body 1, and the power switch is electrically connected to the control module 4, the rotation module, the temperature control module 6 and the observation module 5 to realize power supply and power off. Meanwhile, a communication interface with external equipment is reserved on the outer side of the shell, system upgrading can be performed on a built-in hardware program through the communication interface, and hardware maintenance can be performed on the system.

Fig. 11 shows an eleventh schematic structural diagram of a microfluidic detection device according to an embodiment of the present disclosure; fig. 12 shows a schematic view twelve of a microfluidic detection device according to an embodiment of the present disclosure; please refer to fig. 11 and 12;

the present application provides two specific embodiments;

in a first embodiment, please refer to the microfluidic chip 7 corresponding to fig. 11; a sample cavity 102 in the microfluidic chip 7 is connected with a diluent cavity 101, a capillary valve 108 is arranged between the sample cavity 102 and the diluent cavity 101 and is simultaneously communicated with a downstream mixing cavity 103, the mixing cavity 103 is communicated with a pre-amplification cavity to realize a first amplification reaction in the sample cavity 102, the mixing cavity 103 is communicated with a liquid distribution cavity 104 through a siphon valve 109, a waste liquid cavity 106 is positioned at the downstream of the liquid distribution cavity 104, a gas circulation passage 107 is connected between the waste liquid cavity 106 and the diluent cavity 101 to ensure gas circulation, and the liquid distribution cavity 104 is communicated with a reaction cavity 105. When an experiment is started, adding a sample mixed RPA (recombinase polymerase amplification) reaction solution into a sample cavity 102, adding a diluent into a diluent cavity 101, pre-burying a freeze-dried LAMP (loop-mediated isothermal amplification) reaction reagent in a mixing cavity 103 of a microfluidic chip 7, pre-burying amplification primers aiming at each target in a reaction cavity 105, and sealing a sample port after sample addition; loading the microfluidic chip 7 on a centrifugal microfluidic detector, opening the control software of the moving end, connecting the microfluidic chip to the centrifugal microfluidic detector in a wireless manner, and setting the temperature, the rotation time and the rotation direction of each stage: the first stage, pre-amplification stage, with temperature set at 35 ℃ and heating time of 15 minutes, this stage is not rotated; the second stage, the reagent mixing stage, is at a temperature of 35 ℃, a rotation speed of 1000rpm, a clockwise rotation direction and a rotation time of 60s, and the diluent and the reaction solution after the pre-amplification enter the mixing chamber 103 together under the action of centrifugal force; the third stage, namely a freeze-dried reagent rehydration stage, wherein the temperature is 35 ℃, the rotating speed is accelerated to 2000rpm from 1000rmp, and then is decelerated to 1000rpm from 2000rpm, the cycle is carried out for three times, the rotating direction is clockwise rotation, and the rotating time is 30s; the fourth stage, siphon valve 109 open stage, temperature 35 ℃, rotation speed 0rpm, time 30s; the fifth stage, the reagent dispensing stage, was at a temperature of 35 ℃, a rotational speed of 2000rpm, a rotational direction of counterclockwise rotation, for a period of 60 seconds; the sixth stage, namely the stage that the reagent enters the reaction cavity 105, the temperature is 35 ℃, the rotating speed is 4000rpm, the rotating direction is clockwise rotation, and the rotating time is 30s; the seventh stage, the reaction stage, was at 65 ℃ for 30min and at a rotational speed of 0rpm. After the reaction is set, a mobile terminal starting button is clicked, the experiment is started according to a set rule, in the final reaction stage, the detection module carries out data acquisition every 60s, the acquired data are processed, a real-time amplification curve is displayed on a mobile terminal screen, and of course, signal acquisition can also be carried out after the reaction is finished, and qualitative detection is completed.

In a second embodiment, please refer to the microfluidic chip 7 corresponding to fig. 12; when the experiment starts, adding the sample mixed isothermal amplification reagent into the sample cavity 102, and sealing the sample adding port after sample adding; loading the microfluidic chip 7 on a centrifugal microfluidic detector, opening the control software of the moving end, connecting the microfluidic chip to the centrifugal microfluidic detector in a wireless manner, and setting the temperature, the rotation time and the rotation direction of each stage: first stage-reagent dispensing stage, without heating, with a rotation speed of 2000rpm; second stage-the stage when the reagents enter the reaction chamber 105, the rotation speed is 3500rpm; the third stage, the reaction stage, was at 65 ℃ for 30min. After the reaction is set, a mobile terminal starting button is clicked, the experiment is started according to a set rule, in the final reaction stage, the detection module carries out data acquisition every 60s, the acquired data are processed, a real-time amplification curve is displayed on a mobile terminal screen, and of course, signal acquisition can also be carried out after the reaction is finished, and qualitative detection is completed. If the instrument is used as detection equipment, the above process is designed for detecting specific pathogens for the specific microfluidic chip 7, and the experiment can be directly started by one key; if the instrument is used as experimental equipment, the steps can be added by clicking, and the temperature, time, rotating speed and steering of each step are set to be matched with the micro-fluidic chip 7 developed by the user.

Fig. 13 shows a schematic thirteen structure diagram of a microfluidic detection device according to an embodiment of the present disclosure; please refer to fig. 13;

according to a second aspect of the present disclosure, there is provided a method for detecting a microfluidic chip 7, the method comprising: installing a microfluidic chip 7 containing an object to be detected and a detection agent on a chip loading disc 3 of a microfluidic detection device; the control module 4 of the microfluidic detection device receives a reaction instruction from the mobile terminal device 8; the control module 4 controls the rotating module 2 to drive the chip loading disc 3 to rotate according to the reaction instruction so as to drive the microfluidic chip 7 to rotate, so that the object to be detected and the detection agent are mixed to generate a detection reaction; when the object to be detected and the detection agent are mixed to generate a detection reaction, the control module 4 controls the observation module 5 to observe the microfluidic chip 7 from the specified direction so as to obtain a detection result.

In the embodiment of the disclosure, through the connection between the mobile terminal device 8 and the control module 4, the mobile terminal device 8 can be connected with the control module 4 of the device through wireless communication, the mobile terminal device 8 can set the rotation speed and rotation time of the rotating module 2 in the device, and also can set the temperature and heating time of the temperature control module 6 in the device, and display the real-time rotation speed and temperature of the module, and also can control the detection module to collect data, the mobile terminal device 8 can process the collected data in real time and display the detection result, and meanwhile, the result can be uploaded to a cloud end to cooperate with medical big data analysis; furthermore, the position of the object to be detected in the microfluidic chip 7 can be controlled by controlling the temperature and the rotating speed, so that the position and the reaction stage of the object to be detected can be controlled, and the control on the detection process can be realized. Furthermore, the control module 4 of the device is connected in a wireless manner, and the control module 4 can be controlled by using the mobile terminal device 8, namely, an APP developed on a mobile phone or a software built-in applet, for example, a WeChat applet or a Paibao applet. Thereby realizing the control of the whole device, and the mobile terminal device 8 can monitor the running state of the device and display the final detection result. Meanwhile, a communication interface with external equipment is reserved on the outer side of the shell, and the communication interface can be used for carrying out system upgrade on a built-in hardware program and carrying out hardware overhaul on the system; the shell is also provided with a switch outside to control the on-off between the device and the power supply, the power supply module is positioned outside the shell, and the selectable power supply mode is a mobile power supply or a power line.

In an embodiment, after the control module 4 of the microfluidic detection apparatus receives the reaction instruction from the mobile terminal device 8, the method further includes: the control module 4 controls the temperature control module 6 to regulate the temperature in the temperature control cavity 61 according to the reaction instruction, so as to obtain the temperature control cavity 61 with the specified temperature; the control module 4 controls the rotating module 2 to drive the chip loading disc 3 to rotate according to the reaction instruction so as to drive the microfluidic chip 7 to rotate, so that the object to be detected and the detection agent are mixed in the temperature control cavity 61 at the specified temperature to generate the detection reaction.

It should be understood that various forms of the flows shown above may be used, with steps reordered, added, or deleted. For example, the steps described in the present disclosure may be executed in parallel, sequentially, or in different orders, as long as the desired results of the technical solutions disclosed in the present disclosure can be achieved, and the present disclosure is not limited herein.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In the description of the present disclosure, "a plurality" means two or more unless specifically limited otherwise.

The above description is only for the specific embodiments of the present disclosure, but the scope of the present disclosure is not limited thereto, and any person skilled in the art can easily think of the changes or substitutions within the technical scope of the present disclosure, and shall cover the scope of the present disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.

Claims

1. The microfluidic detection device is characterized by comprising a main body (1), wherein a chip loading disc (3) is arranged on the main body (1), the chip loading disc (3) is used for connecting a microfluidic chip (7), and the microfluidic chip (7) is used for accommodating an object to be detected and a detection agent;

the main body (1) is provided with a rotating module (2), the rotating module (2) is connected with the chip loading disc (3), and the rotating module (2) is used for controlling the chip loading disc (3) to rotate so as to drive the microfluidic chip (7) to rotate, so that an object to be detected is mixed with the detection agent to generate a detection reaction;

the main body (1) is provided with a control module (4), the control module (4) is in communication connection with the rotating module (2), the control module (4) is in communication connection with a mobile terminal device (8), the control module (4) is used for receiving a reaction instruction from the mobile terminal device (8), and the control module (4) is used for controlling the rotating module (2) according to the reaction instruction;

the main body (1) is provided with an observation module (5), the observation module (5) is positioned in the designated direction of the microfluidic chip (7), and the observation module (5) is used for observing the detection reaction of the object to be detected and the detection agent.

2. The device according to claim 1, wherein a temperature control module (6) is further arranged on the main body (1), the temperature control module (6) is formed with a temperature control cavity (61), and the temperature control cavity (61) is used for accommodating the microfluidic chip (7);

the temperature control module (6) is in communication connection with the control module (4), and the control module (4) is used for controlling the temperature of the temperature control cavity (61).

3. The device according to claim 2, characterized in that the temperature control module (6) comprises a cover plate (62), a base plate (63) and a heating element (64);

the cover plate (62) and the bottom plate (63) enclose to form the heating cavity;

the heating element (64) is connected with the cover plate (62), and the heating element (64) is used for heating the cover plate (62) so as to heat the temperature control cavity (61);

and/or the heating element (64) is connected with the bottom plate (63), and the heating element (64) is used for heating the bottom plate (63) so as to heat the temperature control cavity (61).

4. Device according to claim 1, characterized in that the rotating module (2) comprises a drive (21), a transmission (22) and a mounting plate (23),

the driving part (21) passes through the mounting plate (23) with the main part (1) is connected, the driving part (21) passes through the driving part (22) with the chip loading disc (3) is connected, the driving part (21) drives the driving part (22) rotates to drive the chip loading disc (3) to rotate.

5. The device according to claim 1, wherein the chip loading tray (3) comprises a chip tray (31), a slider (32), a stopper (33) and a tray base (34);

a sliding groove (311) is formed in the first surface (313) of the chip tray (31), and the sliding groove (311) is used for the sliding connection of the sliding block (32); the second surface (314) of the chip tray (31) is provided with a limiting groove, and the limiting groove is used for connecting the limiting piece (33);

the limiting piece (33) comprises a connecting portion (331) and a limiting portion (332), the groove is communicated with the sliding groove (311), when the limiting piece (33) is located in the limiting groove, the connecting portion (331) is located in the limiting groove, the limiting portion (332) is located in the sliding groove (311), and the limiting portion (332) is used for abutting against the sliding block (32);

the tray chassis is connected to the second surface (314) of the chip tray (31), and the tray base (34) is used for fastening the limiting piece (33) in the limiting groove; wherein the first surface (313) and the second surface (314) are located differently;

the sliding block (32) is provided with a first inclined surface (321), and an elastic part is arranged between the sliding block (32) and the chip loading disc (3);

under the condition that the mounting hole of the micro-fluidic chip (7) slides along the first inclined surface (321), the sliding block (32) slides towards a first direction under the action of the micro-fluidic chip (7), so that the mounting hole penetrates through the sliding block (32) to be abutted to the chip tray (31); when the microfluidic chip (7) abuts against the chip tray (31), the sliding block (32) abuts against the limiting piece (33) under the action of the elastic piece, and the sliding block (32) is used for fastening the microfluidic chip (7) on the chip tray (31).

6. The device according to claim 5, characterized in that the slider (32) is formed with a second bevel (322), the second bevel (322) being oriented differently from the first bevel (321);

under the condition that the microfluidic chip (7) slides along the second inclined surface (322), the sliding block (32) slides towards a second direction under the action of the microfluidic chip (7), so that the mounting hole penetrates through the sliding block (32) and is separated from the chip tray (31); wherein the first direction and the second direction are different.

7. The apparatus according to claim 4, wherein the chip loading tray (3) is formed with a connection hole (35), the connection hole (35) being for connection of the transmission member (22);

fastening the transmission piece (22) in the connection hole (35) by a fastening member with the transmission piece (22) positioned in the connection hole (35); wherein the transmission member (22) comprises at least a D-shaped shaft (221).

8. The device according to claim 1, characterized in that said body (1) comprises an upper cover (12) and a box (13);

the upper cover (12) is connected with the box body (13) through a hinge piece (11), so that the upper cover (12) can rotate relative to the box body (13) along the hinge piece (11).

9. A microfluidic chip detection method is characterized by comprising the following steps:

when the micro-fluidic chip (7) containing the object to be detected and the detection agent is arranged on the chip loading disc (3) of the micro-fluidic detection device;

a control module (4) of the microfluidic detection device receives a reaction instruction from the mobile terminal equipment (8);

the control module (4) controls the rotating module (2) to drive the chip loading disc (3) to rotate according to the reaction instruction so as to drive the micro-fluidic chip (7) to rotate, so that the object to be detected is mixed with the detection agent to generate a detection reaction;

and under the condition that the object to be detected and the detection agent are mixed to generate a detection reaction, the control module (4) controls the observation module (5) to observe the microfluidic chip (7) from the specified direction so as to obtain a detection result.

10. The method according to claim 9, wherein after the control module (4) of the microfluidic detection device receives the reaction instruction from the mobile terminal device (8), the method further comprises:

the control module (4) controls the temperature control module (6) to adjust the temperature in the temperature control cavity (61) according to the reaction instruction, and the temperature control cavity (61) with the specified temperature is obtained;

the control module (4) controls the rotating module (2) to drive the chip loading disc (3) to rotate according to the reaction instruction so as to drive the micro-fluidic chip (7) to rotate, so that the object to be detected and the detection agent are mixed in the temperature control cavity (61) at the specified temperature, and the detection reaction is carried out.