CN115214997B

CN115214997B - Familial hypercholesterolemia gene detection kit

Info

Publication number: CN115214997B
Application number: CN202210913218.0A
Authority: CN
Inventors: 崔胜男
Original assignee: Jinan Qianmai Medical Laboratory Co ltd
Current assignee: Jinan Qianmai Medical Laboratory Co ltd
Priority date: 2022-08-01
Filing date: 2022-08-01
Publication date: 2023-07-25
Anticipated expiration: 2042-08-01
Also published as: CN115214997A

Abstract

A familial hypercholesterolemia gene detection kit comprises a box body with an upper opening; the box cover is movably connected with the box body; a travel switch is arranged between the box body and the box cover; the wall of the box body is provided with a one-way exhaust valve and a temperature sensor; the upper surface of the box cover is provided with a one-way air inlet valve and a coolant storage bottle; the box cover is provided with an electrical component, the electrical component comprises a power supply module and a circuit module, and the circuit module comprises a display module, a gravity sensor, a temperature sensor, a control module, an electromagnetic valve and a travel switch contact; when the gravity data is larger than the first gravity threshold value and the temperature data is larger than the first temperature data, the control module controls the electromagnetic valve to be opened; when the temperature data is smaller than the second temperature threshold value, the control module controls the electromagnetic valve to be closed; the first temperature threshold is greater than the second temperature threshold; when the temperature data is smaller than the second temperature threshold value, the control module controls the electromagnetic valve to be closed; the first temperature threshold is greater than the second temperature threshold.

Description

Familial hypercholesterolemia gene detection kit

Technical Field

The invention relates to the field of gene detection kits, in particular to a familial hypercholesterolemia gene detection kit.

Background

Familial hypercholesterolemia (Familial hypercholesterolemia, FH) is a hereditary disease characterized by a significant increase in plasma low density lipoprotein cholesterol levels, and other clinical features are marked by significant increases in Total Cholesterol (TC) levels, accumulation of lipids at the skin tendons and arteries, leading to early onset atherosclerosis, increased risk of cardiovascular disease, and coronary heart disease and even myocardial infarction in the juvenile stage of severe cases.

Familial hypercholesterolemia often propagates in the family in an autosomal dominant genetic fashion. There are other genetic forms of hypercholesterolemia caused by mutations in the LDLR, APOB or PCSK9 genes. Existing detection techniques all require the collected biological samples to be stored and transported at low temperatures. However, the existing familial hypercholesterolemia gene detection kit has a simple structure, does not have a refrigeration function, and often fails to preserve biological samples due to the inability to timely refrigerate; the method of compressor refrigeration is adopted, so that the kit is heavy, overlarge in volume, high in power consumption and inconvenient to use.

Therefore, a familial hypercholesterolemia gene detection kit with simple structure, energy saving and refrigeration function needs to be designed.

Disclosure of Invention

In view of the technical problems, the invention provides a familial hypercholesterolemia gene detection kit which has the advantages of simple structure, timely refrigeration, low power consumption, small volume and convenient use.

In order to achieve the above purpose, the technical scheme provided by the invention is as follows: a familial hypercholesterolemia gene detection kit, which comprises a box body with an upper opening; the box cover is movably connected with the box body; the heat preservation layers are arranged in the shells of the box body and the box cover; a travel switch is arranged between the box body and the box cover; the wall of the box body is provided with a one-way exhaust valve and a temperature sensor; the upper surface of the box cover is provided with a one-way air inlet valve and a coolant storage bottle, and the coolant storage bottle, the one-way air inlet valve and the electromagnetic valve are sequentially connected in series on a guide pipe and are communicated with the inside of the box body; the inside of the box body is provided with a reagent bottle and a reagent bottle supporting frame; the box cover is provided with an electrical component, the electrical component comprises a power supply module and a circuit module, and the circuit module comprises a display module, a gravity sensor, a temperature sensor, a control module, a Bluetooth module, an electromagnetic valve and a travel switch contact; the power module is used for supplying power to the electrical component and comprises a storage battery and a charging interface; the display module is used for displaying the temperature inside the box body; the gravity sensor is positioned below the reagent bottle and is used for detecting whether the reagent bottle is loaded with a reagent or not; the control module is used for controlling the opening and closing of the electromagnetic valve and the display module; the electromagnetic valve is positioned on the lower surface of the box cover and connected with the one-way air inlet valve in series through a conduit, and the coolant storage bottle is connected with the one-way air inlet valve through threads; the travel switch contact is closed when the box cover is closed, is opened when the box cover is opened, and is connected in series between the power supply module and the circuit module; the control module is connected with the gravity sensor and the temperature sensor, receives gravity data and temperature data in a split manner, compares the gravity data with a first gravity threshold value, a first temperature threshold value and a second temperature threshold value which are preset in the control module respectively, and controls the electromagnetic valve to be opened when the gravity data is larger than the first gravity threshold value and the temperature data is larger than the first temperature data; when the temperature data is smaller than the second temperature threshold value, the control module controls the electromagnetic valve to be closed; the first temperature threshold is greater than the second temperature threshold; when the temperature data is smaller than the second temperature threshold value, the control module controls the electromagnetic valve to be closed; the first temperature threshold is greater than the second temperature threshold; the Bluetooth module is electrically connected with the control module and sends the temperature condition in the kit in real time.

Preferably, the material of the heat insulation layer is foamed plastic.

Preferably, the one-way exhaust valve and one-way intake valve are remotely located within the familial hypercholesterolemia gene detection kit.

Preferably, the reagent bottle support is not contacted with the bottom of the box body, and the reagent bottle support is provided with a through hole in the vertical direction.

Preferably, the storage battery is a rechargeable lithium battery.

Preferably, the coolant in the coolant storage bottle is dry ice.

Preferably, the coolant storage bottle is threadably connected to the one-way intake valve.

Preferably, the material of the conduit is low temperature alloy copper or low temperature steel.

Preferably, the shell of the box body and the box cover is provided with an inner shell, and the inner shell is made of low-temperature alloy copper or low-temperature steel.

Preferably, the first gravitational threshold = weight of one reagent bottle + weight of the lowest detectable test dose in one reagent bottle; the first temperature threshold = reagent minimum required storage temperature + (reagent maximum required storage temperature-reagent minimum required storage temperature) × 0.618.

The beneficial effects of the invention are as follows:

1. the kit provided by the invention can refrigerate by releasing the coolant, has a refrigerating function, does not need to be placed in a refrigerated cabinet, and can refrigerate in time; no need of compressor, small volume, low weight, low power consumption, simple structure and convenient carrying.

2. The whole circuit is controlled by the travel switch contact, so that refrigeration can not be performed when the box cover is opened, and the cooling agent can be saved; the coolant passes in and out through the one-way air inlet valve and the one-way air outlet valve, and the one-way air inlet valve and the one-way air outlet valve are far away from each other, so that the refrigerant can be fully utilized.

3. The coolant storage bottle is positioned on the box cover and is connected with the one-way air inlet valve through threads, so that the coolant storage bottle is convenient to replace and can meet the requirement of long-distance transportation of reagents.

4. The main electric components are all positioned at the upper part of the box cover, are not influenced by low-temperature environment, do not need to take special measures, and have low cost.

5. According to the invention, by setting the gravity threshold and the temperature threshold, the refrigerant is further saved, and the electricity consumption is reduced.

6. The kit provided by the invention is provided with the Bluetooth module, and the temperature condition in the kit can be sent out in real time through the Bluetooth module, so that a user can know the preservation condition of the reagent in real time even if the user is in principle of the kit.

Drawings

FIG. 1 is a schematic overall view of an embodiment of the present invention

FIG. 2 is a top view of a lid of one embodiment of the present invention

FIG. 3 is a top view of the interior of the case of one embodiment of the present invention

FIG. 4 is a schematic diagram of an electrical assembly according to one embodiment of the invention

FIG. 5 is a solenoid valve control diagram of an embodiment of the invention

In the figure:

1. a case body; 2. a box cover; 3. a reagent bottle holder; 4. a reagent bottle; 10. a one-way exhaust valve; 21. a coolant storage bottle; 22. a conduit; 23. a one-way air inlet valve; 24. a display module; 25. a circuit box; 31. a through hole; 251. a storage battery; 252. a travel switch contact; 253. and a circuit module.

Detailed Description

Familial hypercholesterolemia is a rare autosomal dominant hereditary disease, has familial characteristics, and is characterized by abnormally high low-density lipoprotein (LDL) cholesterol values in the patient, which are 4-6 times higher than normal in the case of syngeneic patients, typically 500-1200mg/dL, even more than 700mg/dL, but normal in triglycerides; also susceptible to early life suffering from cardiovascular disease. The most characteristic clinical manifestations of the disease are increased blood LDL-C level, yellow tumor, corneal arcus and early coronary heart disease. Homozygotes are much more severe in clinical manifestations than heterozygotes. The clinical manifestation of FH patients depends on their genotype, which is also affected by non-genetic factors. Conventional inspection methods often pass through: b ultrasonic examination, 2 coronary angiography, 3 electrocardiography and other relevant physical parameters of human body. Although modern biomedical technology has been developed, gene detection technology has received increasing attention. The existing gene detection of familial hypercholesteremia requires biological samples to be collected and stored at low temperature. The existing kit does not have a refrigeration function, and the kit can not be stored in a refrigerated cabinet after being collected in time in the process of collecting the biological sample, and if the kit needs to be transported, a large amount of electric energy is required to be supplied, so that the kit is very inconvenient.

In view of the above, the present invention provides a familial hypercholesterolemia gene detection kit having a simple structure and a refrigerating function, as shown in fig. 1 to 3, comprising a case 1 having an upper opening; the box cover 2 is movably connected with the box body 1; for example, by hinging the case body 1 to the case cover 2.

The heat preservation layers are arranged in the shells of the box body 1 and the box cover 2; the heat preservation layer is arranged in the interlayer of the shell, so that the consumption of the coolant can be effectively reduced. A travel switch is arranged between the box body 1 and the box cover 2; the travel switch is used for detecting the opening and closing states of the reagent box, the contacts of the travel switch are connected in series in the circuit, and when the box body 1 is in the opening state, the circuit of the reagent box is disconnected, so that the power supply and the coolant can be effectively saved. The wall of the box body 1 is provided with a one-way exhaust valve 10 and a temperature sensor; the one-way exhaust valve 10 is used for exhausting hot air in the box body 1 and preventing external air from entering the space of the kit; the temperature sensor is used for detecting the temperature of the box body 1 and transmitting the detected temperature in the box body 1 to the control module. The upper surface of the box cover 2 is provided with a one-way air inlet valve 23 and a coolant storage bottle 21, and the coolant storage bottle 21, the one-way air inlet valve 23 and an electromagnetic valve are sequentially connected in series on a conduit 22 and are communicated with the inside of the box body 1; the one-way air inlet valve 23 is used for conducting the coolant in the coolant storage bottle into the box body 1 of the reagent box to cool the reagent bottle 4, and the coolant is finally discharged from the one-way air outlet valve 10. The inside of the box body 1 is provided with a reagent bottle 4 and a reagent bottle 4 supporting frame; the reagent bottle 4 is used for loading the collected human biological sample, the reagent bottle support 3 is used for supporting the reagent bottle 4, and the material of the reagent bottle support 3 can be sponge or hard plastic material.

As shown in fig. 4-5, the lid 2 has electrical components which are visually and easily maintained by the benefit of being located on the lid 2, for example, the electrical components may be located within a circuit box 25 of the lid 2. The electrical assembly comprises a power module and a circuit module 253, wherein the circuit module 253 comprises a display module 24, a gravity sensor, a temperature sensor, a control module, a Bluetooth module, an electromagnetic valve and a travel switch contact 252; the power module is used for supplying power to the electrical component and comprises a storage battery 251 and a charging interface; the display module 24 is used for displaying the internal temperature of the kit body 1, and besides, the display can display information such as whether the kit contains reagents or not; further, the display may also have a man-machine interaction function for setting parameter information of the electrical component, or a button is provided on one side of the display, through which parameter setting is performed; the gravity sensors are positioned below the reagent bottles 4, and each gravity sensor is arranged below each reagent bottle 4 and used for detecting whether the reagent bottle 4 is loaded with a reagent and the weight of the reagent and transmitting the weight data to the control module; the control module is used for controlling the opening and closing of the electromagnetic valve and the display module 24, the control module is connected with the gravity sensor and the temperature sensor and receives gravity and temperature data transmitted by the gravity sensor and the temperature sensor, the temperature in the box body 1 is subjected to negative feedback regulation by controlling the opening and closing of the electromagnetic valve, when the box body 1 of the kit is provided with a reagent and the temperature in the box body 1 is too high, the electromagnetic valve is opened for cooling, and when the temperature in the box body 1 of the kit is lower, the electromagnetic valve is closed; the electromagnetic valve is positioned on the lower surface of the box cover 2 and is connected with the one-way air inlet valve 23 in series through a conduit 22; the travel switch contact 252 is closed when the box cover 2 is closed, is opened when the box cover 2 is opened, and is connected in series between the power module and the circuit module 253; thus, the control module can only open the electromagnetic valve to cool the inside of the kit when the box cover 2 is in the closed state, so that electric energy and coolant are not wasted.

The control module is connected with the gravity sensor and the temperature sensor, receives the gravity data and the temperature data in a write-through manner, compares the gravity data with a first gravity threshold value and a first temperature threshold value preset in the control module respectively, and controls the electromagnetic valve to be opened when the gravity data is larger than the first gravity threshold value and the temperature data is larger than the first temperature data; meanwhile, the control module of the kit is also provided with a preset second temperature threshold, and when the temperature data received by the control module is lower than the second temperature threshold, the control module controls the electromagnetic valve to be closed; the first temperature threshold is greater than the second temperature threshold. It should be further noted that, the solenoid valve is in a closed state in the non-electric state; in the power-on state, the opening and closing of the power-on control device are controlled by the control module.

The Bluetooth module is electrically connected with the control module, receives the temperature information transmitted from the control module, and sends the temperature condition in the kit in real time. In this embodiment, the kit may be connected to the mobile terminal through bluetooth, so that a user of the kit may learn about the temperature condition in the kit in real time, and may perform real-time treatment once the abnormal temperature of the kit is found. In this embodiment, the mobile terminal connected to the kit may further have an alarm module, so that the user can be timely reminded to process once an abnormal condition is detected. Meanwhile, as a user of the mobile terminal operator, the kit can be operated through the Bluetooth module, such as setting the threshold temperature and the threshold weight. In this embodiment, a plurality of kits may be connected to one mobile terminal, so that a user can know the temperature conditions in the plurality of kits through one mobile terminal.

Further, the heat insulation layer is made of foamed plastic. The foam plastic has the advantages of light weight, good heat preservation effect and low cost. Therefore, the present invention can simply further reduce the consumption of the coolant.

Further, the one-way exhaust valve 10 and the one-way intake valve 23 are provided remotely in the familial hypercholesterolemia gene detection kit. For example, in the case of the present invention having a cubic structure, the one-way exhaust valve 10 and the one-way intake valve 23 may be disposed substantially opposite to each other along the diagonal of the cube, and this structure can allow the coolant to sufficiently flow in the case 1, thereby improving the coolant utilization rate as much as possible.

Further, the reagent bottle holder 3 is not in contact with the bottom of the case 1, and the reagent bottle holder 3 has a vertically-oriented through hole 31. The reagent bottle holder 3 does not contact with the bottom of the case 1, so as to provide a certain space for the coolant to circulate, and the through holes 31 can provide channels for the coolant to circulate in the case 1 divided between the reagent bottles 4. Further, there may be more than one through hole 31, and at the same time, the through hole 31 may have a spiral structure around the circumference of the reagent bottle 4, further increasing the cooling effect and the utilization rate of the coolant.

Further, the storage battery 251 is a rechargeable lithium battery. The lithium battery has the advantages of light weight and electric quantity.

Further, the coolant in the coolant storage bottle is dry ice. The coolant in the invention is dry ice, the preparation of the dry ice is simple, the price is low, and compared with the coolant such as liquid nitrogen, the coolant meets the cooling requirement and meanwhile the cooling temperature is not too low.

Further, the coolant storage bottle is screwed with the one-way intake valve 23. That is, the coolant storage bottle of the present invention can be simply and conveniently replaced, and since it is communicated with the inside of the cartridge 1 through the one-way intake valve 23, even if the temperature of the cartridge 1 is maintained for a considerable period of time during use, there is enough time for replacing the coolant storage bottle. Of course, the coolant storage bottle and the one-way intake valve 23 of the present invention may be connected by other means, such as a snap-fit connection, a flange connection, etc.

Further, the material of the conduit 22 is low temperature alloy copper or low temperature steel. Conventional materials such as plastics and rubber for the conduit 22 do not have sufficient mechanical strength, but conventional steel materials and aluminum alloys lose ductility at low temperatures, and cannot meet the requirements. Whether the familial hypercholesterolemia genetic test based on the generation I or the generation II genetic test requires preservation of biological materials at low temperatures, such as preservation of blood at temperatures of-20 ℃. It is therefore necessary to select suitable materials for the catheter 22 and the kit. The inventors of the present application found low carbon manganese steel (C0.05-0.28%, mn 0.6-2%). Mn/C is about 10, harmful impurities such as oxygen, nitrogen, sulfur, phosphorus and the like are reduced, and a small amount of elements such as aluminum, niobium, titanium, vanadium and the like are also added to refine grains. The lowest use temperature of the steel is about-60 ℃. The low-temperature copper alloy refers to a copper alloy suitable for use in a low-temperature environment. Copper alloys such as brass and cupronickel are widely used in low-temperature experimental devices and special devices, and have good processing performance.

Further, the shells of the box body 1 and the box cover 2 are provided with inner shells, and the inner shells are made of low-temperature alloy copper or low-temperature steel.

Further, the first gravitational threshold = weight of one reagent bottle 4 + weight of the lowest detectable test dose within one reagent bottle 4. The weight of the reagent bottles 4 corresponding to different detection methods and the lowest detectable test dose meeting the detection requirement are different, the first gravity threshold value in the invention is equal to the weight of one reagent bottle 4 plus the weight of the lowest detectable test dose in one reagent bottle 4, when the gravity sensor under each reagent bottle 4 adds enough reagent in the reagent bottle 4, the control module considers the reagent box to be in a use state, and when the temperature action condition is met, the electromagnetic valve is opened to cool the reagent box.

The first temperature threshold = reagent minimum required storage temperature + (reagent maximum required storage temperature-reagent minimum required storage temperature) x0.618; second temperature threshold = reagent minimum required storage temperature + (reagent maximum required storage temperature-reagent minimum required storage temperature) ×0.2. For example, the minimum required preservation temperature of the reagent is-20 ℃ and the maximum required preservation temperature is-10 ℃, the controller considers that the temperature action condition is met when the temperature is more than-13.82 ℃, and if the weight action condition is met at the same time, the control module controls the electromagnetic valve to be opened to cool the reagent box; if the temperature in the kit body 1 is reduced to-18 ℃, the control module controls the electromagnetic valve to be closed, and cooling is stopped until the temperature rises to the opening temperature of the electromagnetic valve.

In summary, the above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and the related workers can make various changes and modifications without departing from the scope of the technical idea of the present invention. The technical scope of the present invention is not limited to the description, but includes all equivalent changes and modifications in shape, construction, characteristics and spirit according to the scope of the claims.

Claims

1. A familial hypercholesterolemia gene detection kit, characterized in that: the familial hypercholesterolemia gene detection kit comprises a box body with an upper opening;

the box cover is movably connected with the box body;

the heat preservation layers are arranged in the shells of the box body and the box cover;

a travel switch is arranged between the box body and the box cover;

the wall of the box body is provided with a one-way exhaust valve and a temperature sensor;

the upper surface of the box cover is provided with a one-way air inlet valve and a coolant storage bottle, the one-way air inlet valve and the electromagnetic valve are sequentially connected in series on the guide pipe and are communicated with the inside of the box body, and the coolant storage bottle is connected with the one-way air inlet valve through threads;

the inside of the box body is provided with a reagent bottle and a reagent bottle supporting frame;

the box cover is provided with an electrical component, the electrical component comprises a power supply module and a circuit module, and the circuit module comprises a display module, a gravity sensor, a temperature sensor, a control module, a Bluetooth module, an electromagnetic valve and a travel switch contact;

the power module is used for supplying power to the electrical component and comprises a storage battery and a charging interface;

the display module is used for displaying the temperature inside the box body;

the gravity sensor is positioned below the reagent bottle and is used for detecting whether the reagent bottle is loaded with a reagent or not;

the control module is used for controlling the opening and closing of the electromagnetic valve and the display module;

the electromagnetic valve is positioned on the lower surface of the box cover and is connected with the one-way air inlet valve in series through a conduit;

the travel switch contact is closed when the box cover is closed, is opened when the box cover is opened, and is connected in series between the power supply module and the circuit module;

the control module is connected with the gravity sensor and the temperature sensor, receives gravity data and temperature data in a split manner, compares the gravity data with a first gravity threshold value, a first temperature threshold value and a second temperature threshold value which are preset in the control module respectively, and controls the electromagnetic valve to be opened when the gravity data is larger than the first gravity threshold value and the temperature data is larger than the first temperature data; when the temperature data is smaller than the second temperature threshold value, the control module controls the electromagnetic valve to be closed; the first temperature threshold is greater than the second temperature threshold;

the Bluetooth module is electrically connected with the control module and sends the temperature condition in the kit in real time;

the one-way exhaust valve and the one-way intake valve are arranged in the familial hypercholesterolemia gene detection kit in a far away manner;

the reagent bottle support is not contacted with the bottom of the box body, and a through hole in the vertical direction is formed in the reagent bottle support;

the coolant in the coolant storage bottle is dry ice;

the first gravitational threshold = weight of one reagent bottle + weight of the lowest detectable test dose in one reagent bottle; the first temperature threshold = reagent minimum required storage temperature + (reagent maximum required storage temperature-reagent minimum required storage temperature) × 0.618.

2. The familial hypercholesterolemia gene detection kit according to claim 1, wherein:

the heat insulation layer is made of foamed plastic.

3. The familial hypercholesterolemia gene detection kit according to claim 1, wherein:

the storage battery is a rechargeable lithium battery.

4. The familial hypercholesterolemia gene detection kit according to claim 1, wherein:

the material of the conduit is low-temperature alloy copper or low-temperature steel.

5. The familial hypercholesterolemia gene detection kit according to claim 1, wherein:

the shell of the box body and the box cover is provided with an inner shell, and the inner shell is made of low-temperature alloy copper or low-temperature steel.