CN1152128A - Fully automatic integral detector for blood viscosity and deformation and aggregation of red blood cells - Google Patents
Fully automatic integral detector for blood viscosity and deformation and aggregation of red blood cells Download PDFInfo
- Publication number
- CN1152128A CN1152128A CN 96104678 CN96104678A CN1152128A CN 1152128 A CN1152128 A CN 1152128A CN 96104678 CN96104678 CN 96104678 CN 96104678 A CN96104678 A CN 96104678A CN 1152128 A CN1152128 A CN 1152128A
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- 210000004369 blood Anatomy 0.000 title claims abstract description 41
- 239000008280 blood Substances 0.000 title claims abstract description 41
- 238000004220 aggregation Methods 0.000 title claims description 20
- 230000002776 aggregation Effects 0.000 title claims description 20
- 210000003743 erythrocyte Anatomy 0.000 title description 9
- 239000011521 glass Substances 0.000 claims abstract description 19
- 238000012360 testing method Methods 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 12
- 238000002834 transmittance Methods 0.000 description 4
- 239000000725 suspension Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005755 formation reaction Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 101150064138 MAP1 gene Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 238000002050 diffraction method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000000518 rheometry Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
The present invention is a detector for the detection of rheological behaviour of blood in medicine and is an integral automatic one including blood viscometer and conic plate diffraction instrument. The heart of the said detector is a rotating conic plate diffruction unit comprising rotating conic plate driven by the viscometer and glass plate. With no blood sample pollution and reduced contact chance of operator to blood sample, the said detector can ensure the safety of operator.
Description
The invention belongs to the determining instrument field of medicinal blood rheological properties.
Blood rheology parameter such as the blood viscosity of macroscopic view and the red cell deformability and the aggregation of microcosmic, has important clinical diagnostic significance and medical research and is worth.Red blood cell is the important visible component of blood, and is uniquely in the human body can make things convenient for the active somatic cell of obtaining in a large number.Can whether red blood cell has good deformability, determining red blood cell pass through capillary smoothly, keeps little perfusion that follows, and keeps the human body normal physiological function; Erythrocytic aggregation influences the blood viscosity of blood under low shear rate to a great extent.Red blood cell is stiff and assemble, and must damage capillary and cause various microvascular diseases, and the blood viscosity of macroscopic view is the macro manifestations of red cell deformability/aggregation.Measure the more advanced assay method easily of red cell deformability/aggregation at present laser diffractometry and the political reform of cone-plate rotating flow are arranged.The former rotates the red blood cell that is diluted by suspending liquid in the transparent concentric garden post viscosity meter with laser radiation, measures the long and short axle of its red blood cell diffraction ring; And the latter sees through to stand on the rotation clear glass flat board, glass neck plate along the axle rotation, be clipped in the erythrocytic individual deformability of dilution between glass plate and glass cone-plate with microscopic examination, and blood viscosity directly from rotational viscosimeter torsion torque sensor obtain viscosity parameter.At present, viscosity meter (as Chinese patent 95201524.2) is surveyed blood viscosity, and cone-plate diffractometer (as Chinese patent 93211081.9) is surveyed red cell deformability and aggregation, and two instrument splits divide task, do not support mutually.
The object of the invention is to develop blood viscometer and red cell deformability/aggregation cone-plate diffractometer, supports one another and integrated full automatic determinator.
The present invention seeks to realize like this: the torsion torque sensor of top viscosity meter drives glass cone-plate 1 rotation of bottom rotation cone-plate diffractometer, viscosity meter is obtained the blood viscosity bulk parameter on glass plate 3, obtain erythrocytic deformability/aggregation micro-parameter from glass cone-plate diffractometer, two class parameters are sent into computer respectively, carry out data processing and test result and export on demand.
Below in conjunction with figure explanation content of the present invention;
Fig. 1 workflow diagram of the present invention;
Fig. 2 fundamental diagram of the present invention;
Fig. 3 viscosity meter structural drawing;
Fig. 4 test result curve map of the present invention.
As seen from Figure 1, " the application of sample flusher " of wriggling sample-adding pump formation is responsible for sample is sent automatically Entering core apparatus of the present invention " rotation cone-plate diffracting object " and diffracting object thereof works with afterflush. " temperature Controller " keep the diffracting object working environment to be stabilized in 25～37 ℃ ± 0.1 ℃. " rotation cone-plate diffracting object " Blood viscosity part in the data receives and converts to the signal of telecommunication by " viscosimetric analysis ", and red blood cell becomes in the data Shape property/aggregation part by/" RCD/aggregation is measured " receive and convert to the signal of telecommunication. Blood The viscosity signal of telecommunication and deformability deformability of cells/aggregation signal of telecommunication is sent into " computer data processing " again and is carried out the data place Reason and storage and printout.
As seen from Figure 2, core of the present invention is glass cone-plate 1 and glass plate 3 formations of rotation " rotation cone-plate diffracting object " is full of blood sample 2 in the diffracting object. Top viscosimeter rotating shaft driven rotary cone-plate 1 rotation, the blood viscosity physical quantity by its torsion torque sensor records converts the signal of telecommunication to through " viscosimetric analysis "; The laser beam that laser instrument produces is vertically injected glass plate 3, sees through the diffraction that blood sample 2 and rotation cone-plate 1 produce Light is received by " light target ", and its diffraction pattern send " diffraction imaging " to convert the signal of telecommunication to. Because suspension In particle to be in the time of can loosing state light transmittance littler, particle aggregation then whole suspension light transmittance on the contrary Increase, so light target removes and receive cell diffraction pattern part, also received suspension (being blood sample) Light transmittance, the light transmittance signal of telecommunication treated the aggregation data, and diffraction pattern is RCD Data.
Fig. 3 is viscosimeter structure chart (patent No. CN95201524.2), and the E among the figure on the rotating shaft turns round The power sensor, this sensor is obtained the physical quantity of blood viscosity data.
Synthesizing map 1, Fig. 2, Fig. 3, the rotation of viscosity meter driven rotary cone-plate diffracting object is by the bulk parameter of viscosity meter taking blood sample, i.e. blood viscosity; By the micro-parameter of diffracting object taking blood sample, i.e. deformability deformability of cells/aggregation data.Viscosity meter and rotation cone-plate diffractometer are supported mutually, once produce two class parameters, and handle through computer, obtain accurate data rapidly.In view of the above integrated full-automatic blood viscosity and deformability deformability of cells/aggregation determinator.This installs each blood sample and only needs 1.5ml amount and 3 fens clock times, and torsion is determined as 0.5-100mPas (centipoise) rotating speed at 0-200
-3(second
-1).This device has reduced contacting of operating personnel and blood preparation, ensures operating personnel's safety, and prevents that blood sample from polluting, and has eliminated the intervention of personal error simultaneously, has improved the blood testing precision.
Embodiment 1 surveys blood viscosity
Make the rotary glass cone-plate rotation of diffracting object, the ICS1008 type torsion torque sensor on the viscosity meter record the viscosity of blood sample between the rotary glass cone-plate of diffracting object and the glass plate with viscosity meter (CN95201524.2) turning axle.Concrete mounting structure is seen Fig. 2.Blood sampling 1ml sends into the space between glass cone-plate and the glass plate automatically, and recording blood sample is 1ml, and spended time 3 minutes records the viscosity number under the different shearings rate.See Fig. 4 (a)
Blood sample is 1ml, 3 minutes consuming time, records the maximum distortion rate, angle.See Fig. 4 (b)
Embodiment surveys blood viscosity and deformability deformability of cells/aggregation
Blood sample is 1ml, 3 minutes consuming time, record blood viscosity and deformability deformability of cells/aggregation, and see Fig. 4 (c).
Claims (1)
1, a kind of blood viscosity red cell deformability fully automatic integral determinator, it is characterized in that the torsion torque sensor of top viscosity meter drives glass cone-plate 1 rotation of bottom rotation cone-plate diffractometer, viscosity meter is obtained the blood viscosity bulk parameter on glass plate 3, obtain erythrocytic deformability/aggregation micro-parameter from glass cone-plate diffractometer, two class parameters are sent into computer respectively, carry out data processing and test result and export on demand.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96104678 CN1074130C (en) | 1996-04-17 | 1996-04-17 | Fully automatic integral detector for blood viscosity and deformation and aggregation of red blood cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96104678 CN1074130C (en) | 1996-04-17 | 1996-04-17 | Fully automatic integral detector for blood viscosity and deformation and aggregation of red blood cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1152128A true CN1152128A (en) | 1997-06-18 |
| CN1074130C CN1074130C (en) | 2001-10-31 |
Family
ID=5118444
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 96104678 Expired - Fee Related CN1074130C (en) | 1996-04-17 | 1996-04-17 | Fully automatic integral detector for blood viscosity and deformation and aggregation of red blood cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1074130C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1866006B (en) * | 2006-06-13 | 2011-12-21 | 北京航空航天大学 | Microscopically-observable cone-plate shear field generator |
| CN102998219A (en) * | 2012-12-24 | 2013-03-27 | 常州大学 | Cone plate-flat plate clamp of rotational rheometer |
| CN101713775B (en) * | 2003-04-24 | 2014-12-10 | 科拉梅德科技有限公司 | Method for measuring blood sample resonance frequency and sealed container for holding blood sample |
| CN107741384A (en) * | 2017-10-23 | 2018-02-27 | 常州大学 | Cone-plate plate jig for rotational rheometer |
| CN112710587A (en) * | 2020-12-23 | 2021-04-27 | 山东大学 | Cone plate rotary type high-viscosity non-Newtonian fluid viscometer and viscosity detection method |
-
1996
- 1996-04-17 CN CN 96104678 patent/CN1074130C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101713775B (en) * | 2003-04-24 | 2014-12-10 | 科拉梅德科技有限公司 | Method for measuring blood sample resonance frequency and sealed container for holding blood sample |
| CN1866006B (en) * | 2006-06-13 | 2011-12-21 | 北京航空航天大学 | Microscopically-observable cone-plate shear field generator |
| CN102998219A (en) * | 2012-12-24 | 2013-03-27 | 常州大学 | Cone plate-flat plate clamp of rotational rheometer |
| CN102998219B (en) * | 2012-12-24 | 2014-12-31 | 常州大学 | Cone plate-flat plate clamp of rotational rheometer |
| CN107741384A (en) * | 2017-10-23 | 2018-02-27 | 常州大学 | Cone-plate plate jig for rotational rheometer |
| CN112710587A (en) * | 2020-12-23 | 2021-04-27 | 山东大学 | Cone plate rotary type high-viscosity non-Newtonian fluid viscometer and viscosity detection method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1074130C (en) | 2001-10-31 |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| C14 | Grant of patent or utility model | ||
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| ASS | Succession or assignment of patent right |
Owner name: DOMINO( BEIJING )SCIENTIFIC INSTRUMENT IMPORT CO. Free format text: FORMER OWNER: LI GANG Effective date: 20041029 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20041029 Address after: 100085, No. 29, East Road, Beijing, Haidian District Patentee after: Domino (Beijing) Scientific Instrument Co., Ltd. Address before: 100080 iron research No. 136, College Road, Beijing, Haidian District Patentee before: Li Gang |
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| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |