CN115201498A - Reagent card for blood coagulation detection and detection instrument - Google Patents
Reagent card for blood coagulation detection and detection instrument Download PDFInfo
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- CN115201498A CN115201498A CN202110391279.0A CN202110391279A CN115201498A CN 115201498 A CN115201498 A CN 115201498A CN 202110391279 A CN202110391279 A CN 202110391279A CN 115201498 A CN115201498 A CN 115201498A
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- blood coagulation
- pawl
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- reagent card
- bottom plate
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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Abstract
The invention discloses a reagent card and a detection instrument for blood coagulation detection, wherein the reagent card comprises a pawl mechanism and a blood coagulation bottom plate; a plurality of detection chambers are arranged on the blood coagulation bottom plate at intervals; the pawl mechanism is rotatably arranged on the blood coagulation bottom plate, and sampling pawls extending into the detection chambers are arranged on the pawl mechanism; still be provided with the application of sample groove on the blood coagulation bottom plate, the application of sample groove communicates to each detection cavity through dividing the appearance runner. The reagent card and the detection instrument for detecting the blood coagulation disclosed by the invention can realize multi-project synchronous detection by utilizing the matching of each detection chamber and each rotating sampling pawl, and effectively improve the detection efficiency.
Description
Technical Field
The invention relates to a reagent card and a detection instrument, in particular to a reagent card and a detection instrument for blood coagulation detection.
Background
At present, the existing reagent card test items are single, only one blood coagulation claw is used, multiple tests cannot be synchronously performed, and the test efficiency is low; in addition, the existing test method of the reagent card is to judge the blood coagulation (threshold value) by irradiating the blood coagulation claw on the side surface and blocking the light from passing through the coagulation of the blood clot, and if the blood coagulation is not uniform, the detection is inaccurate. Therefore, there is a need to design a reagent card and a detection apparatus for blood coagulation detection, which can realize multiple synchronous detections and have high detection accuracy.
Disclosure of Invention
The purpose of the invention is as follows: the reagent card and the detection instrument for detecting the blood coagulation can realize multiple synchronous detections and have high detection precision.
The technical scheme is as follows: the invention relates to a reagent card for blood coagulation detection, which comprises a pawl mechanism and a blood coagulation bottom plate; a plurality of detection chambers are arranged on the blood coagulation bottom plate at intervals; the pawl mechanism is rotatably arranged on the blood coagulation bottom plate and comprises sampling pawls extending into the detection chambers; the blood coagulation bottom plate is also provided with a sample adding groove, and the sample adding groove is communicated to each detection chamber through sample dividing runners.
Further, in order to improve the liquid separation precision, the height of the bottom of the sample adding groove is higher than that of the inlet of the detection chamber, and the sample separation flow channel is a slope ramp.
Furthermore, the pawl mechanism is formed by sequentially butting a plurality of pawl units; the pawl unit comprises a central rotating shaft and the sampling pawl, and the sampling pawl is sleeved on the central rotating shaft and can rotate relative to the central rotating shaft.
Furthermore, the sampling pawl is composed of a lantern ring arranged on the central rotating shaft and a plurality of pawls arranged on the circumferential surface of the lantern ring at equal intervals.
Furthermore, the front end of the central rotating shaft of the foremost pawl unit is provided with a driving jack, and the front ends of the central rotating shafts of the other pawl units are provided with transmission insertion blocks; the rear end of the central rotating shaft of each pawl unit is provided with a transmission notch; each pawl unit is inserted into the corresponding transmission notch through the transmission insertion block to realize the butt joint in sequence. The pawl units which are closely arranged are connected in a one-to-one nested mode, and redundant transmission mechanisms are avoided.
Furthermore, all be provided with a support on the blood coagulation bottom plate and be located the side of each detection cavity, be provided with the arc mounting groove on the support, the rotation type of center pivot is installed on the arc mounting groove.
Furthermore, each collection strip-shaped groove is formed in the blood coagulation bottom plate, and each detection chamber is respectively sunken in the bottom of each collection strip-shaped groove.
The invention also provides a detection instrument for detecting blood coagulation, wherein an optical detector is arranged above the reagent card for detecting blood coagulation, and the optical detector comprises:
a light source generating device for generating incident light;
the dichroic mirror is obliquely arranged above the detection chamber and used for reflecting incident light to a sample to be detected on the sampling pawl;
and the fluorescence receiver is positioned above the dichroic mirror and used for collecting a fluorescence signal generated by a sample to be detected on the sampling pawl.
The invention uses fluorescence excitation method to detect, and judges the blood coagulation time through the change of the excitation light.
Furthermore, the dichroic mirror is obliquely arranged above the detection chamber at an angle of 45 degrees and used for reflecting incident light to a sample to be detected on the sampling pawl at an angle of 90 degrees.
Compared with the prior art, the invention has the beneficial effects that: by matching each detection chamber with each rotating sampling pawl, multi-project synchronous detection can be realized, and the detection efficiency is effectively improved; by utilizing the matching of the sample adding slot and the sample dividing flow channel, the synchronous sample conveying to each detection chamber can be realized.
Drawings
FIG. 1 is a schematic diagram of a reagent card according to the present invention;
FIG. 2 is a schematic view of a coagulation base plate according to the present invention;
FIG. 3 is a schematic view of the detent mechanism of the present invention;
FIG. 4 is a schematic view of the detecting apparatus of the present invention.
Detailed Description
The technical solution of the present invention is described in detail below with reference to the accompanying drawings, but the scope of the present invention is not limited to the embodiments.
Example 1:
as shown in fig. 1 to 4, the reagent card for blood coagulation test according to the present invention comprises: a pawl mechanism and a coagulation base plate 1; a plurality of detection chambers 7 are arranged on the blood coagulation bottom plate 1 at intervals; the pawl mechanism is rotatably arranged on the blood coagulation bottom plate 1 and comprises sampling pawls extending into the detection chambers 7; the blood coagulation bottom plate 1 is also provided with a sample adding groove 2, and the sample adding groove 2 is communicated to each detection chamber 7 through a sample dividing flow channel 3.
When the pawl mechanism rotates, the sampling pawl can stir a sample to be detected in the detection chamber and can hook up a clot in the sample to be detected in the detection chamber; by matching each detection chamber 7 with each rotating sampling pawl, multi-project synchronous detection can be realized, and the detection efficiency is effectively improved; by utilizing the matching of the sample adding slot 2 and the sample separating flow channel 3, the synchronous sample conveying to each detection chamber 7 can be realized.
Further, the height of the bottom of the sample adding groove 2 is higher than the height of the inlet of the detection chamber 7, and the sample separation flow channel 3 is a slope ramp. With the arrangement of the ramp, the sample can be facilitated to flow into the detection chamber 7 quickly.
Furthermore, the pawl mechanism is formed by sequentially butting a plurality of pawl units; the pawl unit comprises a central rotating shaft 11 and the sampling pawl, and the sampling pawl is sleeved on the central rotating shaft 11 and can rotate relative to the central rotating shaft 11. The pawl units are sequentially butted to form the pawl mechanism, so that the number of the pawl mechanisms can be expanded according to detection requirements, and the pawl mechanism has strong adaptability.
Further, the sampling pawl is composed of a lantern ring 12 arranged on the central rotating shaft 11 and a plurality of pawls 13 arranged on the circumferential surface of the lantern ring 12 at equal intervals, the pawls 13 are L-shaped, so that a sample to be detected can be smoothly displayed below the detection light, each pawl 13 is arranged on the circumferential surface of the lantern ring 12 at equal intervals in sequence, and in this embodiment, the number of the pawls 13 is eight. By the arrangement of the multi-pawl 13, multiple detections can be performed during rotation per period, and the detection precision is further improved; the axial position of each pawl unit can be limited by the collar 12.
Furthermore, the front end of the central rotating shaft 11 of the foremost pawl unit is provided with a driving jack 8, and the front ends of the central rotating shafts 11 of the rest pawl units are provided with transmission insertion blocks 16; the rear end of the central rotating shaft 11 of each pawl unit is provided with a transmission notch 15; each pawl unit is inserted into the corresponding transmission slot 15 through the transmission insert 16 to realize sequential butt joint. The driving jack 8 can be butted with a driving plug 10 at the end part of a power input shaft 9, so that the rotary driving of the pawl mechanism is realized; by means of the matching of the transmission insert block 16 and the transmission notch 15, the butt transmission of each pawl unit can be realized, and the pawl mechanism is convenient to expand.
Further, a support 4 is arranged on the blood coagulation bottom plate 1 and on the side edge of each detection chamber 7, an arc-shaped mounting groove 5 is arranged on the support 4, and the central rotating shaft 11 is rotatably mounted on the arc-shaped mounting groove 5; the lantern ring 12 is clamped between two adjacent support seats 4 in a limiting way. The entrance of the arc-shaped mounting groove 5 is set to be a slightly narrowed structure, and the support 4 is made of plastic materials with certain elasticity, so that the central rotating shaft 11 can be narrowed when inserted, and the central rotating shaft 11 is limited.
Further, be provided with each on the blood coagulation bottom plate 1 and collect bar groove 6, each detection chamber 7 is sunken the setting respectively on each groove bottom of collecting bar groove 6. The utilization is collected bar groove 6 and can be at the rotatory sampling in-process of sample pawl, carries out spacing backward flow to the sample in detecting chamber 7, prevents that the sample from spilling over.
The invention also provides a detecting instrument for detecting blood coagulation, wherein an optical detector is arranged above the reagent card for detecting blood coagulation, and the optical detector comprises:
a light source generating device for generating incident light;
the dichroic mirror 20 is obliquely arranged above the detection chamber and used for reflecting incident light to a sample to be detected on the sampling pawl;
and the fluorescence receiver 19 is positioned above the dichroic mirror 20 and is used for collecting a fluorescence signal generated by the sample to be detected on the sampling pawl.
The incident light can be reflected by the dichroic mirror 20 and the reflected light can be transmitted, so that the reflected light is collected by the fluorescence receiver 19.
Further, the dichroic mirror 20 is obliquely installed above the detection chamber 7 at an angle of 45 ° and is configured to reflect incident light onto a sample to be detected on the sampling pawl at an angle of 90 °.
In the reagent card for detecting blood coagulation, the blood coagulation bottom plate 1 can be made of hydrophilic plastics, or a hydrophilic reagent is coated in the sample separation flow channel 3; during production, two reagents, namely a fluorescent reagent and a blood coagulation reagent, are stored in the bottom of the detection chamber 7 in a freeze-dried manner, and when no liquid sample exists, the two reagents are kept in a solidified state and have a certain distance with the upper sampling pawl, so that the two reagents cannot be scraped by the sampling pawl to be carried to the upper side for detection, and misjudgment is avoided.
When the reagent card for blood coagulation detection is used, firstly, a detection instrument is electrified and started, the reagent card is inserted, incident light is reflected to the surface of the pawl 13 through 90 degrees of the dichroic mirror 20, and if a sample to be detected on the surface of the sampling pawl has a fluorescent reagent, excited light returns, passes through the dichroic mirror 20 and is collected by the fluorescent receiver 19; when no sample is added, the excitation light is very weak; when the sample flows into the detection chamber 7, the freeze-dried reagent is quickly dissolved into the sample under the stirring of the sampling pawl, more and more blood clots with the fluorescent reagent are arranged on the surface of the sampling pawl, and the exciting light is gradually enhanced; when blood coagulation is finished, a blood clot with a large amount of fluorescent reagents is formed on the surface of the sampling pawl, the exciting light reaches the strongest level and tends to be stable in a subsequent period of time, the detection instrument captures the change of the intensity of the exciting light through the fluorescent receiver 19 to judge the blood coagulation time, the intensity of the exciting light starts to rise on a drawn map as a starting point, and the excitation intensity rises to the highest level and tends to be stable as an end point, so that the detection precision is high.
As noted above, while the present invention has been shown and described with reference to certain preferred embodiments, it is not to be construed as limited to the invention itself. Various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (9)
1. A reagent card for use in coagulation assays, comprising: comprises a pawl mechanism and a blood coagulation bottom plate (1); a plurality of detection chambers (7) are arranged on the blood coagulation bottom plate (1) at intervals; the pawl mechanism is rotatably arranged on the blood coagulation bottom plate (1), and comprises sampling pawls extending into the detection chambers (7); the blood coagulation bottom plate (1) is also provided with a sample adding groove (2), and the sample adding groove (2) is communicated to each detection chamber (7) through a sample dividing flow channel (3).
2. The reagent card for blood coagulation detection according to claim 1, wherein: the bottom of the sample adding groove (2) is higher than the inlet of the detection chamber (7), and the sample separating flow channel (3) is a slope ramp.
3. The reagent card for blood coagulation detection according to claim 1, wherein: the pawl mechanism is formed by sequentially butting a plurality of pawl units; the pawl unit comprises a central rotating shaft (11) and the sampling pawl, and the sampling pawl is sleeved on the central rotating shaft (11) and can rotate relative to the central rotating shaft (11).
4. The reagent card for blood coagulation detection according to claim 3, wherein: the sampling pawl is composed of a lantern ring (12) arranged on a central rotating shaft (11) and a plurality of pawls (13) arranged on the circumferential surface of the lantern ring (12) at equal intervals.
5. The reagent card for blood coagulation detection according to claim 3, wherein: the front end of the central rotating shaft (11) of the foremost pawl unit is provided with a driving jack (8), and the front ends of the central rotating shafts (11) of the rest pawl units are provided with transmission insertion blocks (16); the rear end of the central rotating shaft (11) of each pawl unit is provided with a transmission notch (15); each pawl unit is inserted into the corresponding transmission notch (15) through a transmission insertion block (16) to realize sequential butt joint.
6. The reagent card for blood coagulation detection according to claim 3, wherein: the blood coagulation bottom plate is characterized in that a support (4) is arranged on the side edge of each detection chamber (7) on the blood coagulation bottom plate (1), an arc-shaped mounting groove (5) is formed in the support (4), and the central rotating shaft (11) is rotatably mounted on the arc-shaped mounting groove (5).
7. The reagent card for blood coagulation detection according to claim 1, wherein: be provided with each on blood coagulation bottom plate (1) and collect bar groove (6), each detection chamber (7) is sunken the setting respectively on the tank bottom portion of each collection bar groove (6).
8. A test instrument for the detection of blood coagulation, characterized by: an optical detector is disposed above the reagent card for blood coagulation detection according to any one of claims 1 to 7, the optical detector comprising:
a light source generating device for generating incident light;
the dichroic mirror (20) is obliquely arranged above the detection chamber and is used for reflecting incident light to a sample to be detected on the sampling pawl;
and the fluorescence receiver (19) is positioned above the dichroic mirror (20) and is used for collecting a fluorescence signal generated by the sample to be detected on the sampling pawl.
9. A test instrument for coagulation testing according to claim 8 wherein: the dichroic mirror (20) is obliquely arranged above the detection chamber (7) at an angle of 45 degrees and used for reflecting incident light to a sample to be detected on the sampling pawl at an angle of 90 degrees.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110391279.0A CN115201498A (en) | 2021-04-13 | 2021-04-13 | Reagent card for blood coagulation detection and detection instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110391279.0A CN115201498A (en) | 2021-04-13 | 2021-04-13 | Reagent card for blood coagulation detection and detection instrument |
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| Publication Number | Publication Date |
|---|---|
| CN115201498A true CN115201498A (en) | 2022-10-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110391279.0A Pending CN115201498A (en) | 2021-04-13 | 2021-04-13 | Reagent card for blood coagulation detection and detection instrument |
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| CN (1) | CN115201498A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115684140A (en) * | 2023-01-05 | 2023-02-03 | 江苏协鲲生物技术有限责任公司 | Reagent card, homogeneous phase chemiluminescence detection system and homogeneous phase chemiluminescence detection method |
-
2021
- 2021-04-13 CN CN202110391279.0A patent/CN115201498A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115684140A (en) * | 2023-01-05 | 2023-02-03 | 江苏协鲲生物技术有限责任公司 | Reagent card, homogeneous phase chemiluminescence detection system and homogeneous phase chemiluminescence detection method |
| CN115684140B (en) * | 2023-01-05 | 2023-08-15 | 江苏协鲲生物技术有限责任公司 | Reagent card, homogeneous chemiluminescence detection system and homogeneous chemiluminescence detection method |
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