CN115193789B - Elisa plate cleaning method and elisa instrument equipment with cleaning structure - Google Patents
Elisa plate cleaning method and elisa instrument equipment with cleaning structure Download PDFInfo
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- CN115193789B CN115193789B CN202211125239.2A CN202211125239A CN115193789B CN 115193789 B CN115193789 B CN 115193789B CN 202211125239 A CN202211125239 A CN 202211125239A CN 115193789 B CN115193789 B CN 115193789B
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- 238000004140 cleaning Methods 0.000 title claims abstract description 189
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000002965 ELISA Methods 0.000 claims abstract description 253
- 230000007246 mechanism Effects 0.000 claims abstract description 130
- 238000001035 drying Methods 0.000 claims abstract description 128
- 239000007788 liquid Substances 0.000 claims abstract description 64
- 238000012360 testing method Methods 0.000 claims abstract description 7
- 238000005406 washing Methods 0.000 claims description 43
- 238000012546 transfer Methods 0.000 claims description 41
- 238000013519 translation Methods 0.000 claims description 36
- 238000003825 pressing Methods 0.000 claims description 24
- 230000032258 transport Effects 0.000 claims description 23
- 102000004190 Enzymes Human genes 0.000 claims description 19
- 108090000790 Enzymes Proteins 0.000 claims description 19
- 230000000712 assembly Effects 0.000 claims description 17
- 238000000429 assembly Methods 0.000 claims description 17
- 238000005086 pumping Methods 0.000 claims description 10
- 230000005540 biological transmission Effects 0.000 claims description 9
- 230000002093 peripheral effect Effects 0.000 claims description 4
- 238000010008 shearing Methods 0.000 claims description 3
- 230000007723 transport mechanism Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 3
- 238000005119 centrifugation Methods 0.000 description 18
- 241000252254 Catostomidae Species 0.000 description 14
- 230000033001 locomotion Effects 0.000 description 13
- 230000008569 process Effects 0.000 description 8
- 230000007306 turnover Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 238000009434 installation Methods 0.000 description 4
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- 230000004048 modification Effects 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002457 bidirectional effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000001680 brushing effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
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- 238000011160 research Methods 0.000 description 1
- 238000011012 sanitization Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
Images
Classifications
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- B08B1/12—
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- B08B1/32—
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G47/00—Article or material-handling devices associated with conveyors; Methods employing such devices
- B65G47/74—Feeding, transfer, or discharging devices of particular kinds or types
- B65G47/90—Devices for picking-up and depositing articles or materials
- B65G47/91—Devices for picking-up and depositing articles or materials incorporating pneumatic, e.g. suction, grippers
- B65G47/915—Devices for picking-up and depositing articles or materials incorporating pneumatic, e.g. suction, grippers provided with drive systems with rotary movements only
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G47/00—Article or material-handling devices associated with conveyors; Methods employing such devices
- B65G47/74—Feeding, transfer, or discharging devices of particular kinds or types
- B65G47/90—Devices for picking-up and depositing articles or materials
- B65G47/91—Devices for picking-up and depositing articles or materials incorporating pneumatic, e.g. suction, grippers
- B65G47/918—Devices for picking-up and depositing articles or materials incorporating pneumatic, e.g. suction, grippers with at least two picking-up heads
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B5/00—Drying solid materials or objects by processes not involving the application of heat
- F26B5/08—Drying solid materials or objects by processes not involving the application of heat by centrifugal treatment
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
Abstract
The invention discloses an ELISA plate cleaning method and an ELISA plate device with a cleaning structure, and the method comprises a cleaning machine, an ELISA plate and an ELISA plate; the cleaning machine comprises an ELISA plate grabbing mechanism, an ELISA plate transferring and placing platform, an ELISA plate transferring mechanism, an ELISA plate spin-drying tank body, an ELISA plate cleaning mechanism, an ELISA plate centrifugal spin-drying mechanism and an outer tank body. The ELIASA equipment with the cleaning structure provided by the invention can automatically clean the ELIASA, and adopts an automatic implementation mode before and after cleaning to automatically transport the ELIASA to the cleaning position, and the cleaned ELIASA is automatically transported back to the ELIASA, so that the manual intervention is reduced, the time of testing personnel is saved, the adopted ELIASA cleaning structure can comprehensively clean the inner wall of the plate hole of the ELIASA, and can realize spin-drying after cleaning, so that the residual liquid in the ELIASA plate can be reduced to the maximum extent, and the cleaning effect of the ELIASA plate cleaning machine is improved.
Description
Technical Field
The invention belongs to the technical field of detection and analysis equipment, and particularly relates to a method for cleaning an ELISA plate. The invention also relates to an ELIASA device with a cleaning structure for the ELIASA plate cleaning method.
Background
The ELISA plate washing machine is mainly used for equipment for washing a micropore plate in enzyme-linked immunity, time resolution and chemiluminescence experiments, is widely applied to hospitals, blood stations, epidemic prevention stations, research rooms, reagent factories and the like, and is specially used for washing the ELISA plate.
Present most wash the trigger when wasing the ELIAS plate, all only wash the surface, do not soak the surface that just washs the ELIAS plate, lead to the inside residue of ELIAS plate to be difficult to clear away, and the ELIAS plate is inconvenient when wasing, and inconvenient follow is torn open on washing board equipment simultaneously, remains liquid on the ELIAS plate after the sanitization, causes and washs not thoroughly.
Disclosure of Invention
The purpose of the invention is as follows: in order to overcome the defects, the invention aims to provide a method for cleaning an ELISA plate, which solves the problems that in the washing process of the ELISA plate washing machine in the prior art, a small amount of residual liquid is attached to the edges of the inner wall and the bottom of the hole plate, so that the washing is incomplete, the washing needs to be carried out for multiple times, or workers need to assist in washing.
The technical scheme is as follows: an ELIASA device with a cleaning structure comprises a cleaning machine, an ELIASA and an ELIASA, wherein the cleaning machine is positioned at one side of the ELIASA, the ELIASA is arranged on the ELIASA, and the cleaning machine is used for cleaning the ELIASA; the washing machine comprises an ELISA plate grabbing mechanism, an ELISA plate transferring and placing platform, an ELISA plate transferring mechanism, an ELISA plate spin-drying tank body, an ELISA plate washing mechanism, an ELISA plate centrifugal spin-drying mechanism and an outer tank body, wherein the ELISA plate grabbing mechanism, the ELISA plate transferring and placing platform, the ELISA plate transferring mechanism, the ELISA plate spin-drying tank body and the ELISA plate washing mechanism are all arranged in the outer tank body, and the ELISA plate centrifugal spin-drying mechanism is arranged in the ELISA plate spin-drying tank body; the elisa plate grabbing mechanism transfers the elisa plate between the elisa plate reader and the elisa plate transfer and placing platform; the ELIAS plate transferring mechanism transfers the ELIAS plate between the ELIAS plate transferring platform and the ELIAS plate centrifugal drying mechanism; the ELISA plate cleaning mechanism is used for cleaning the ELISA plate on the ELISA plate centrifugal spin-drying mechanism; and the ELISA plate centrifugal drying mechanism is used for performing centrifugal drying on the ELISA plate. The ELIASA equipment with the cleaning structure provided by the invention can automatically clean the ELIASA, adopts an automatic realization mode before and after cleaning, automatically transports the ELIASA to the cleaning position, automatically transports the cleaned ELIASA back to the ELIASA, reduces manual intervention, saves the time of testing personnel, reduces the damage to the testing personnel in the cleaning process, adopts the ELIASA cleaning structure, can comprehensively clean the inner wall of a plate hole of the ELIASA by imitating a mode of manually brushing a test tube, can realize spin-drying after cleaning, can furthest reduce residual liquid in the ELIASA plate, and improves the cleaning effect of the ELIASA plate cleaning machine.
Furthermore, foretell ELIASA equipment that has clean structure, the ELIASA snatchs the mechanism and snatchs the manipulator including snatching the support base, snatching reciprocating drive device, translation straight line module, translation linear guide, translation straight line slider, Z type backup pad and ELIASA, it fixes on the bottom plate of outer box internal portion to snatch the support base, snatch reciprocating drive device and set up on snatching the support base, translation straight line module and translation linear guide all set up on snatching reciprocating drive device to translation linear guide and the moving direction parallel arrangement of translation straight line module, translation straight line slider and translation straight line guide sliding connection, the one end of Z type backup pad is connected with the slider and the translation straight line slider of translation straight line module, the ELIASA snatchs the manipulator and sets up on the other end of Z type backup pad, the ELIASA snatchs the manipulator and transports the ELIASA between ELIASA and ELIASA transportation placing platform. The ELIAS plate that sets up snatchs the mechanism and constructs through snatching the bidirectional movement structure that reciprocating drive device and translation straight line module constitute, can realize the ELIAS plate at the motion of two vertical directions to in the automatic immigration ectonexine of ELIAS plate, above-mentioned process goes on automatically, has reduced tester's work load.
Further, foretell ELIASA equipment that has clean structure, ELIASA transfer mechanism is including transporting horizontal drive device, transporting vertical drive device and a set of ELIASA sucking disc two, transport horizontal drive device and set up on the roof of outer box internal portion, transport vertical drive device and transport horizontal drive device and connect, a set of ELIASA sucking disc two is connected with transporting vertical drive device, the both ends of transporting horizontal drive device direction of movement are located the ELIASA respectively and transport place the platform and ELIASA spin-dry jar body top. The ELIAS plate transfer mechanism realizes the conversion between the ELIAS plate placing position ELIAS plate cleaning positions through horizontal linear motion, and can realize that a set of ELIAS plate sucking discs two reach ELIAS plate position department through the vertical drive arrangement of transportation that sets up, realizes getting of ELIAS plate and puts.
Further, the microplate reader equipment with the cleaning structure comprises a vertical driving device, two symmetrically arranged linear slide rails II, two symmetrically arranged linear guide rails III, a screw rod supporting seat II, a vertical driving screw rod, a screw rod nut II, a vertical sliding plate I, a push arm II and two symmetrically arranged vertical guide posts II, wherein the vertical driving motor is connected with the vertical driving screw rod, the vertical driving screw rod is arranged on the screw rod supporting seat II, the two symmetrically arranged linear slide rails II, the two symmetrically arranged linear guide rails III, the screw rod supporting seat II and the two symmetrically arranged vertical guide posts II are all arranged on a supporting frame, a slide block II is arranged on the linear slide rail II, a connecting plate I is connected between the slide blocks II, and a slide block III is arranged on the two symmetrically arranged linear guide rails III, a connecting plate II is connected between the third sliding block, a lead screw nut II is in threaded connection with a vertical driving lead screw, a vertical sliding plate I is fixedly connected with the lead screw nut II, a push arm I and a push arm II are arranged in a V shape, the cross point position of the push arm I and the push arm II is hinged with the lead screw nut II, the end part of the push arm I, far away from the lead screw nut II, is hinged with the connecting plate I, the end part of the push arm II, far away from the lead screw nut II, is hinged with the connecting plate II, two vertical guide posts II which are symmetrically arranged are arranged in parallel with the vertical driving lead screw, the vertical driving lead screw is positioned between two vertical guide posts II which are symmetrically arranged, the vertical sliding plate I is in sliding connection with the two vertical guide posts II which are symmetrically arranged, a shear fork mechanism is connected between the connecting plate I and the connecting plate II, and the lower end part of the shear fork mechanism is connected with a sucker mounting plate, and a group of second enzyme label plate suckers are arranged on the sucker mounting plate and adsorb the enzyme label plate. The vertical driving device for transferring drives the vertical driving screw rod to rotate through the vertical driving motor, the rotation of the vertical driving screw rod is converted into linear movement of the screw nut II, and the first pushing arm and the second pushing arm which are arranged in a V shape and connected with the screw nut II are used for driving the driving structure of the scissor mechanism to extend or retract the scissor mechanism, so that the lifting of the second ELISA plate sucking disc is realized in the telescopic process of the scissor mechanism.
Furthermore, foretell ELIASA equipment that has clean structure, ELIASA wiper mechanism is including wasing support frame body, washing vertical drive arrangement, a set of belt cleaning device and liquid suction pipe, the roof fixed connection of washing support frame body and outer box internal portion, wash vertical drive arrangement and set up on wasing support frame body, a set of belt cleaning device is a setting on wasing vertical drive arrangement, the liquid suction pipe setting is wasing vertical drive arrangement and is connected, the one end and the ELIASA centrifugal drying mechanism of liquid suction pipe are connected to the other end and the pump of liquid suction pipe are connected. The principle of transferring the vertical driving device is adopted to clean the vertical driving device, a group of cleaning devices can be lowered in place, the group of cleaning devices respectively correspond to one row of ELISA plate holes, the cleaning devices synchronously clean one row of ELISA plate holes, and cleaning liquid is recycled through the liquid suction pipe.
Further, foretell ELIASA equipment that has clean structure, belt cleaning device includes that a set of washing brush subassembly, brush are rotatory to be driven actuating cylinder, cylinder installation support body, rotatory drive rack, a set of rotary drive gear and a set of rotation axis, cylinder installation support body fixed connection, the rotatory actuating cylinder that drives of brush sets up on cylinder installation support body to the rotatory actuating cylinder that drives of brush and the rotatory rack connection that drives of drive, rotatory drive rack and cylinder installation support body sliding connection to rotatory drive rack and a set of rotary drive gear one of them meshing, a set of rotary drive gear is the one-row setting, and mesh between a set of rotary drive gear, a set of washing brush subassembly, a set of rotary drive gear and a set of rotation axis one-to-one set up, rotary drive gear and the upper end connection of rotation axis, washing brush subassembly and rotation axis connection, a set of belt cleaning device's a set of washing brush subassembly sets up according to the mode of rectangular array to a set of washing brush subassembly and the diaphragm orifice one-to-one setting of ELIASA board of a set of belt cleaning device. The brush rotation driving cylinder drives the group of rotating shafts to rotate synchronously through the transmission of the rotation driving rack and the group of rotation driving gears, so that the cleaning brush assembly connected with the rotating shafts makes circular motion around the rotating shafts.
Further, foretell ELIASA equipment that has clean structure, it includes clamp, vertical connecting plate, washing brush, the vertical actuating cylinder, brush mounting bracket and the washing nozzle of driving of brush to wash the brush subassembly, clamp and axis of rotation are connected, vertical connecting plate and clamp connection, the vertical actuating cylinder setting that drives of brush is on vertical connecting plate to the vertical piston rod and the brush mounting bracket that drive the actuating cylinder of driving of brush are connected, wash the brush and all set up on the brush mounting bracket with the washing nozzle, and wash the brush and stretch into the inboard of ELIAS plate with the washing nozzle. The vertical piston rod that drives actuating cylinder of brush slowly washs the brush and is circular motion round the rotation axis in, slowly stretches out or withdraws to make and wash the brush and go up and down in the diaphragm orifice of ELIAS plate, the washing route of wasing the brush is the helix, still makes in addition that the washing brush reaches the downthehole different degree position department of ELIAS plate diaphragm orifice, including the bottom in ELIAS plate diaphragm orifice, has guaranteed the cleaning performance of ELIAS plate.
Further, foretell ELIASA equipment that has clean structure, ELIASA centrifugation spin-dry mechanism includes centrifugation spin-dry base, centrifugation spin-dry driving motor, centrifugation spin-dry action wheel, centrifugation spin-dry from driving wheel, drive chain, centrifugation spin-dry pivot, rotor plate, the ELIASA placer and the support frame that spin-dries that two symmetries set up, the centrifugation spin-dry base is fixed to be set up on the bottom plate of the ELIASA spin-dry jar body, centrifugation spin-dry driving motor and the fixed setting on the centrifugation spin-dry base of support frame, centrifugation spin-dry driving motor and centrifugation spin-dry action wheel are connected, the centrifugation spin-dry action wheel passes through drive chain and centrifugation spin-dry from the driving wheel connection, the centrifugation is connected from driving wheel and centrifugation spin-dry pivot, centrifugation spin-dry pivot and rotor plate connection, the both ends setting of rotor plate are on the support frame, the ELIASA placer setting that two symmetries set up is on the support frame. The elisa plate centrifugal spin-drying mechanism can be used for switching the two elisa plate placing devices which are symmetrically arranged, and simultaneously, after the elisa plate is finished, the two elisa plate placing devices which are symmetrically arranged are rotated at a high speed to spin-dry the elisa plate.
Further, foretell ELIASA equipment that has clean structure, ELIASA board placer includes that a set of ELIASA board compresses tightly subassembly, liquid reserve tank, ELIASA board rectangle braced frame and sharp module two, sharp module two sets up on the rotor plate, the slider fixed connection of liquid reserve tank and sharp module two, a set of ELIASA board compresses tightly the subassembly and sets up around ELIASA board rectangle braced frame, ELIASA board rectangle braced frame is fixed to be set up on the inside bottom plate of liquid reserve tank to the upper end of ELIASA board rectangle braced frame stretches out the liquid reserve tank, a set of ELIASA board compresses tightly the subassembly and compresses tightly the edge all around of ELIASA board on ELIASA board rectangle braced frame, be equipped with the clearance between the outer wall of ELIASA board rectangle braced frame and the inner wall of liquid reserve tank, the one end setting of liquid suction pipe is in the clearance, be equipped with a plurality of through-holes on the ELIASA board rectangle braced frame, inside through a plurality of through-hole and liquid reserve tank intercommunication. Along with abluent continuous going on, the washing liquid spills over in the stock solution box, retrieves through the liquid suction pipe, and a set of ELIAS plate compresses tightly the subassembly and sets up around the ELIAS plate rectangle braced frame of rectangular cross section, can compress tightly the ELIAS plate on ELIAS plate rectangle braced frame steadily.
The invention also provides a method for cleaning an ELISA plate of the ELISA reader equipment, the method comprises the following steps:
s1, pushing the elisa plate which is tested out of the elisa plate by the elisa plate tester;
s2, automatically opening a box door of the outer box body;
s3, extending the elisa plate grabbing mechanism out of the outer box body, and grabbing an elisa plate on the elisa plate reader by the elisa plate grabbing mechanism;
s4, retracting the ELISA plate grabbing mechanism into the outer box body;
s5, placing the ELISA plate on an ELISA plate transferring and placing platform by the ELISA plate grabbing mechanism;
s6, moving the ELISA plate on the ELISA plate transferring and placing platform to an ELISA plate spin-drying tank body by the ELISA plate transferring mechanism, and placing the ELISA plate in the ELISA plate spin-drying tank body by the ELISA plate transferring mechanism;
s7, cleaning the ELISA plate on the ELISA plate spin-drying tank body by the ELISA plate cleaning mechanism;
s8, after the ELISA plate is cleaned, closing an automatic door on the ELISA plate spin-drying tank body, and performing centrifugal spin-drying on the ELISA plate by using the ELISA plate spin-drying tank body;
s9, taking out the centrifugally-dried ELISA plate by the ELISA plate transferring mechanism, and transferring the ELISA plate to an ELISA plate transferring and placing platform;
s10, an ELISA plate grabbing mechanism grabs the ELISA plate on the ELISA plate transferring and placing platform and moves the ELISA plate out of the outer box body;
s11, placing the ELISA plate on the ELISA reader by the ELISA plate grabbing mechanism.
The technical scheme shows that the invention has the following beneficial effects: the microplate reader equipment with the cleaning structure has the following advantages:
1. the cleaning mechanism of the ELISA plate can completely clean the inner wall of the hole of the ELISA plate by simulating the process of manually cleaning the test tube and arranging the cleaning brush which does circular motion to rub the hole of the ELISA plate in the rotating process of the cleaning brush, thereby improving the cleaning effect of the ELISA plate and avoiding residues;
2. the arranged ELIAS plate centrifugal spin-drying mechanism is provided with two ELIAS plate placing structures, cleaning of two ELIAS plates can be completed by switching in turn, and the cleaned ELIAS plates are spin-dried at a high speed, so that the cleaning efficiency is remarkably improved compared with the conventional cleaning mode of manually placing the ELIAS plates in a tray;
3. the ELIAS plate that sets up snatchs mechanism and ELIAS plate transport mechanism and adopts automatic mode, realizes the automatic transportation of ELIAS plate to realize that above-mentioned cleaning process goes on automatically, reduced artificial intervention, reduced testing personnel's work load.
Drawings
FIG. 1 is a schematic diagram of a microplate reader device having a cleaning structure according to the present invention;
FIG. 2 is a schematic view of the cleaning machine according to the present invention;
FIG. 3 is a schematic structural diagram of an ELISA plate grabbing mechanism according to the present invention;
FIG. 4 is a schematic structural view of the grasping reciprocation drive unit according to the present invention;
FIG. 5 is a schematic structural diagram of an ELISA plate transfer mechanism according to the present invention;
FIG. 6 is a schematic view of the configuration of the transfer vertical drive of the present invention;
FIG. 7 is a schematic view of a portion of the transfer vertical drive of the present invention;
FIG. 8 is a schematic structural diagram of an ELISA plate cleaning mechanism according to the present invention;
FIG. 9 is a bottom view of the microplate cleaning mechanism of the present invention;
FIG. 10 is a schematic view of the cleaning apparatus according to the present invention;
FIG. 11 is a side view of the microplate cleaning mechanism of the present invention;
FIG. 12 is a schematic structural view of an ELISA plate centrifugal drying mechanism according to the present invention;
FIG. 13 is a schematic structural view of an ELISA plate placement device according to the present invention;
FIG. 14 is a top view of an microplate placement device according to the invention;
FIG. 15 is a front view of the outer case of the present invention;
fig. 16 is a left side view of the outer case of the present invention.
In the figure: ELISA plate grabbing mechanism 1, grabbing support base 11, grabbing reciprocating drive device 12, reciprocating drive motor 121, reciprocating drive gear 122, reciprocating drive rack 123, horizontal sliding plate 124, sliding block 125, linear sliding rail 126, translational linear module 13, translational linear guide rail 14, translational linear sliding block 15, Z-shaped support plate 16, ELISA plate grabbing manipulator 17, grabbing drive cylinder 171, grabbing turnover support seat 172, turnover support plate 173, Y-shaped connection plate 174, grabbing turnover plate 175, ELISA plate sucker 176, ELISA plate transferring platform 2, ELISA plate transferring mechanism 3, transferring horizontal drive device 31, transferring horizontal drive motor 311, transferring horizontal drive screw 312, screw support seat 313, screw nut 314, horizontal sliding plate 315, guide column 316, support frame 317, transferring vertical drive device 32, vertical drive motor 320, linear sliding rail 321, guide column 316, support frame 317 a linear guide rail III 322, a screw rod supporting seat II 323, a vertical driving screw rod 324, a screw rod nut II 325, a vertical sliding plate I326, a push arm I327, a push arm II 328, a vertical guide column II 329, a sliding block II 3210, a connecting plate I3211, a sliding block III 3212, a connecting plate II 3213, a scissor mechanism 3214, a sucking disc mounting plate 3215, an ELISA plate sucking disc II 33, an ELISA plate spin-drying tank body 4, an automatic box door I41, an automatic box door II 42, an ELISA plate cleaning mechanism 5, a cleaning supporting frame body 51, a cleaning vertical driving device 52, a cleaning mounting plate 520, a cleaning device 53, a cleaning brush assembly 531, a hoop 5311, a vertical connecting plate 5312, a cleaning brush 5313, a brush vertical driving cylinder 5314, a brush mounting frame 5315, a cleaning nozzle 5316, a brush rotation driving cylinder 532, a cylinder mounting frame body 533, a rotation driving rack 534, a rotation driving gear 535, a rotation shaft 536, a liquid pumping pipe 54, a rotation driving device and a liquid pump, the elisa plate centrifugal drying device comprises an elisa plate centrifugal drying mechanism 6, a centrifugal drying base 61, a centrifugal drying driving motor 62, a centrifugal drying driving wheel 63, a centrifugal drying driven wheel 64, a transmission chain 65, a centrifugal drying rotating shaft 66, a rotating plate 67, an elisa plate placing device 68, an elisa plate compressing assembly 681, a compressing driving cylinder 6811, a compressing fixed supporting seat 6812, a compressing turning rod 6813, a compressing head 6814, a liquid storage box 682, an elisa plate rectangular supporting frame 683, a linear module II 684, a drying supporting frame 69, an outer box 7, a box body base 71, a box body 72, a lifting door 73, a lifting door body 731, a lifting driving motor 732, a lifting supporting frame 733, a lifting driving screw rod 734, a lifting guide plate 735, a cleaning machine 100, an elisa plate instrument 200 and an elisa plate 300.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "clockwise", "counterclockwise", and the like, indicate orientations or positional relationships based on those shown in the drawings, merely for convenience of description and simplicity of description, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, unless otherwise specified, "a plurality" means two or more unless explicitly defined otherwise.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.
In the present invention, unless expressly stated or limited otherwise, the recitation of a first feature "on" or "under" a second feature may include the recitation of the first and second features being in direct contact, and may also include the recitation that the first and second features are not in direct contact, but are in contact via another feature between them. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
Examples
The microplate reader equipment with the cleaning structure shown in fig. 1 and 2 comprises a cleaning machine 100, a microplate reader 200 and an microplate 300, wherein the cleaning machine 100 is positioned at one side of the microplate reader 200, the microplate 300 is arranged on the microplate reader 200, and the cleaning machine 100 cleans the microplate 300; the cleaning machine 100 comprises an ELISA plate grabbing mechanism 1, an ELISA plate transferring and placing platform 2, an ELISA plate transferring mechanism 3, an ELISA plate spin-drying tank body 4, an ELISA plate cleaning mechanism 5, an ELISA plate centrifugal spin-drying mechanism 6 and an outer tank body 7, wherein the ELISA plate grabbing mechanism 1, the ELISA plate transferring and placing platform 2, the ELISA plate transferring mechanism 3, the ELISA plate spin-drying tank body 4 and the ELISA plate cleaning mechanism 5 are all arranged in the outer tank body 7, and the ELISA plate centrifugal spin-drying mechanism 6 is arranged in the ELISA plate spin-drying tank body 4; the ELISA plate grabbing mechanism 1 transports an ELISA plate 300 between an ELISA reader 200 and an ELISA plate transporting and placing platform 2; the ELIAS plate transferring mechanism 3 transfers the ELIAS plate 300 between the ELIAS plate transferring and placing platform 2 and the ELIAS plate centrifugal spin-drying mechanism 6; the ELISA plate cleaning mechanism 5 is used for cleaning the ELISA plate 300 on the ELISA plate centrifugal drying mechanism 6; the ELISA plate centrifugal drying mechanism 6 carries out centrifugal drying on the ELISA plate 300.
Based on the structure, the working method of the microplate reader device with the cleaning structure comprises the following steps:
s1, pushing the elisa plate 300 which is tested out of the elisa plate reader 200 by the elisa plate reader 200;
s2, automatically opening a box door of the outer box body 7;
s3, the ELISA plate grabbing mechanism 1 extends out of the outer box body 7, and the ELISA plate grabbing mechanism 1 grabs the ELISA plate 300 on the ELISA reader 200;
s4, retracting the ELISA plate grabbing mechanism 1 into the outer box body 7;
s5, placing the ELISA plate 300 on the ELISA plate transferring and placing platform 2 by the ELISA plate grabbing mechanism 1;
s6, the ELIAS plate transferring mechanism 3 moves the ELIAS plate 300 on the ELIAS plate transferring and placing platform 2 to the ELIAS plate spin-drying tank body 4, and the ELIAS plate transferring mechanism 3 places the ELIAS plate 300 in the ELIAS plate spin-drying tank body 4;
s7, cleaning the ELISA plate 300 on the ELISA plate spin-drying tank body 4 by the ELISA plate cleaning mechanism 5;
s8, after the ELISA plate 300 is cleaned, the automatic door on the ELISA plate spin-drying tank body 4 is closed, and the ELISA plate spin-drying tank body 4 carries out centrifugal spin-drying on the ELISA plate 300;
s9, taking out the centrifugally-dried ELISA plate 300 by the ELISA plate transferring mechanism 3, and transferring the ELISA plate 300 to the ELISA plate transferring and placing platform 2;
s10, the ELISA plate grabbing mechanism 1 grabs the ELISA plate 300 on the ELISA plate transferring and placing platform 2 and moves the ELISA plate out of the outer box body 7;
s11, the microplate capture mechanism 1 places the microplate 300 on the microplate reader 200.
The microplate grabbing mechanism 1 shown in fig. 3 comprises a grabbing support base 11, a grabbing reciprocating drive device 12, a translation linear module 13, a translation linear guide rail 14, a translation linear slider 15, a Z-shaped support plate 16 and an microplate grabbing manipulator 17, wherein the grabbing support base 11 is fixedly arranged on a bottom plate inside an outer box body 7, the grabbing reciprocating drive device 12 is arranged on the grabbing support base 11, the translation linear module 13 and the translation linear guide rail 14 are both arranged on the grabbing reciprocating drive device 12, the translation linear guide rail 14 is arranged in parallel with the moving direction of the translation linear module 13, the translation linear slider 15 is in sliding connection with the translation linear guide rail 14, one end of the Z-shaped support plate 16 is connected with a slider of the translation linear module 13 and the translation linear slider 15, the microplate grabbing manipulator 17 is arranged at the other end of the Z-shaped support plate 16, and the microplate grabbing manipulator 17 is used for transporting and placing the microplate 300 between the microplate reader 200 and the microplate transporting and the placing platform 2. Snatch reciprocal drive arrangement 12 and can drive the ELIAS plate and snatch manipulator 17 and stretch out or withdraw outer box 7, snatch when the ELIAS plate snatch manipulator 17 and stretch out outer box 7, the ELIAS plate snatchs manipulator 17 and can follow ELIAS marker 200 and take off the ELIAS plate 300 of treating the washing, snatch reciprocal drive arrangement 12 and will snatch ELIAS plate 300 and withdraw outer box 7, it snatchs manipulator 17 and transports place the platform 2 directions and remove to the ELIAS plate along translation linear guide 14 to drive the ELIAS plate through translation linear module 13, and finally place ELIAS plate 300 and transport on the ELIAS plate transports place the platform 2 and wait to transport.
The grabbing reciprocating driving device 12 shown in fig. 4 comprises a reciprocating driving motor 121, a reciprocating driving gear 122, a reciprocating driving rack 123, a first horizontal sliding plate 124, a group of first sliders 125 and two first parallel linear sliding rails 126, wherein a rotating shaft of the reciprocating driving motor 121 is connected with the reciprocating driving gear 122, the reciprocating driving gear 122 is meshed with the reciprocating driving rack 123, the reciprocating driving rack 123 is connected with the first horizontal sliding plate 124, a group of first sliders 125 are arranged on the lower end surface of the first horizontal sliding plate 124, the two first parallel linear sliding rails 126 are arranged in parallel with the reciprocating driving rack 123, the reciprocating driving rack 123 is located between the two first parallel linear sliding rails 126, the first sliders 125 are connected with the first linear sliding rails 126 in a sliding manner, and the moving direction of the reciprocating driving rack 123 is perpendicular to the moving direction of the translational linear module 13. The rotating shaft of the reciprocating driving motor 121 rotates to drive the reciprocating driving gear 122 to rotate forwards or reversely, so that the reciprocating driving rack 123 reciprocates along the horizontal direction, and the elisa plate grabbing manipulator 17 can move linearly.
In the above structure, the elisa plate grabbing manipulator 17 comprises a grabbing driving cylinder 171, a grabbing turning support seat 172, a turning support plate 173, a Y-shaped connecting plate 174, a grabbing turning plate 175 and a set of elisa plate suckers 176, the grabbing driving cylinder 171 is hinged to the Z-shaped support plate 16, the grabbing turning support seat 172 is fixedly arranged on the Z-shaped support plate 16, the turning support plate 173 and the grabbing turning support seat 172 are fixedly connected, three connection points of the Y-shaped connecting plate 174 are respectively connected with a piston rod of the grabbing driving cylinder 171, the turning support plate 173 and the grabbing turning plate 175, and the set of elisa plate suckers 176 is arranged on the grabbing turning plate 175. The piston rod which is used for grabbing the driving cylinder 171 extends out or retracts, the grabbing turnover plate 175 can be driven to rotate reversely by a certain angle, so that the ELISA plate 300 is grabbed in an adaptive mode, and the ELISA plate 300 is placed conveniently.
The ELIAS plate transfer mechanism 3 shown in FIG. 5 comprises a transfer horizontal driving device 31, a transfer vertical driving device 32 and a set of ELIAS plate sucking discs two 33, the transfer horizontal driving device 31 is arranged on a top plate inside the outer box body 7, the transfer vertical driving device 32 is connected with the transfer horizontal driving device 31, the set of ELIAS plate sucking discs two 33 are connected with the transfer vertical driving device 32, and the two ends of the transfer horizontal driving device 31 in the moving direction are respectively positioned above the ELIAS plate transfer placing platform 2 and the ELIAS plate spin-drying tank body 4. The horizontal driving device 31 and the vertical driving device 32 are transferred to form a double-shaft motion system, horizontal and vertical motion is achieved respectively, the horizontal movement enables a group of two ELISA plate suckers 3 to be switched between the ELISA plate transfer placing platform 2 and the ELISA plate centrifugal drying mechanism 6, and the ELISA plate 300 is transferred.
In the above structure, the transfer horizontal driving device 31 includes a transfer horizontal driving motor 311, a transfer horizontal driving screw 312, two symmetrically arranged screw supporting seats 313, a set of screw nuts 314, a horizontal sliding plate 315, two symmetrically arranged guiding columns 316 and a supporting frame 317, the transfer horizontal driving motor 311 is connected with the transfer horizontal driving screw 312, the transfer horizontal driving screw 312 is arranged on the two symmetrically arranged screw supporting seats 313, the set of screw nuts 314 is in threaded connection with the transfer horizontal driving screw 312, the horizontal sliding plate 315 is fixedly connected with one of the set of screw nuts 314, the horizontal sliding plate 315 is in sliding connection with the two symmetrically arranged guiding columns 316, the two symmetrically arranged guiding columns 316 and the transfer horizontal driving screw 312 are arranged in parallel, the transfer horizontal driving screw 312 is located between the two symmetrically arranged guiding columns 316, the supporting frame 317 is fixedly connected with the screw nuts 314 and the horizontal sliding plate 315, and the transfer vertical driving device 32 is connected with the supporting frame 317. The both ends of transporting horizontal drive device 31 direction of movement are located the ELIAS plate respectively and transport place the platform 2 and the ELIAS plate centrifugation 6 tops that spin-dry constructs, transport horizontal drive motor 311 drive like this and transport horizontal drive lead screw 312 rotatory for when lead screw nut 314 removes the both ends of transporting horizontal drive lead screw 312 axis direction, horizontal sliding plate two 315 is located the ELIAS plate respectively and transports place the platform 2 and the ELIAS plate centrifugation directly over mechanism 6, realize transporting.
The transfer vertical driving device 32 shown in fig. 6 and 7 includes a vertical driving motor 320, two symmetrically arranged linear sliding rails two 321, two symmetrically arranged linear guide rails three 322, a screw rod supporting seat two 323, a vertical driving screw rod 324, a screw rod nut two 325, a vertical sliding plate one 326, a push arm one 327, a push arm two 328 and two symmetrically arranged vertical guide posts two 329, the vertical driving motor 320 is connected with the vertical driving screw rod 324, the vertical driving screw rod 324 is arranged on the screw rod supporting seat two 323, the two symmetrically arranged linear sliding rails two 321, the two symmetrically arranged linear guiding rails three 322, the screw rod supporting seat two 323 and the two symmetrically arranged vertical guide posts two 329 are all arranged on a supporting frame 317, the linear sliding rails two 321 are provided with sliders two 3210, a connecting plate one 3211 is connected between the sliders two 3210, the sliders three symmetrically arranged linear guiding rails 322 are provided with sliders three 3212, a connecting plate II 3213 is connected between the third sliding block 3212, the second screw nut 325 is in threaded connection with the vertical driving screw 324, the first vertical sliding plate 326 is fixedly connected with the second screw nut 325, the first push arm 327 and the second push arm 328 are arranged in a V shape, the intersection of the first push arm 327 and the second push arm 328 is hinged with the second screw nut 325, the end of the first push arm 327, far away from the second screw nut 325, is hinged with a connecting plate I3211, the end of the second push arm 328, far away from the second screw nut 325, is hinged with a connecting plate II 3213, the two symmetrically arranged vertical guide posts II 329 and the vertical driving screw 324 are arranged in parallel, the vertical driving screw 324 is arranged between the two symmetrically arranged vertical guide posts II 329, the first vertical sliding plate 326 is in sliding connection with the two symmetrically arranged vertical guide posts II 329, a shearing fork mechanism 3214 is connected between the connecting plate I3211 and the connecting plate II 3213, the lower end of the scissor mechanism 3214 is connected with a suction cup mounting plate 3215, a set of two elisa plate suction cups 33 is arranged on the suction cup mounting plate 3215, and the set of two elisa plate suction cups 33 adsorbs the elisa plate 300.
The working principle of the transfer vertical driving device 32 is as follows:
the vertical driving motor 32 is started to drive the vertical driving screw rod 324 to rotate, so that the screw nut II 325 moves along the axis direction of the vertical driving screw rod 324, the position of the screw nut II 325 is changed, the V-shaped opening between the pushing arm I327 and the pushing arm II 328 is pushed to expand or contract, the pushing arm I327 and the pushing arm II 328 move, the sliding block II 3210 slides along the linear sliding rail II 321, the sliding block III 3212 slides along the linear guiding rail III 322, the V-shaped opening between the pushing arm I327 and the pushing arm II 328 expands or contracts, the distance between the connecting plate I3211 and the connecting plate II 3213 is changed, the scissor mechanism 3214 is driven to stretch, the sucker mounting plate 3215 is changed in the height direction due to the stretching of the scissor mechanism 3214, the lifting of a group of two elisa plates 33 is realized, and the taking and placing of the elisa plates 300 are carried out and placed in the lifting process of the group of the sucker plates and sucker plates.
In the structure, the elisa plate drying tank body 4 is of a rectangular box-shaped structure, and the upper end part of the elisa plate drying tank body 4 is provided with a first automatic box door 41 and a second automatic box door 42. Through opening or closing of the first automatic box door 41 and the second automatic box door 42, the elisa plate spin-drying tank body 4 can be closed, so that spin-drying is achieved, cleaning liquid is prevented from being sprayed out, the first automatic box door 41 and the second automatic box door 42 are opened, the elisa plate 300 can be placed in or taken out, and the elisa plate cleaning mechanism 5 stretches into the elisa plate spin-drying tank body 4 to be cleaned.
The microplate cleaning mechanism 5 shown in fig. 8 and 9 comprises a cleaning support frame body 51, a cleaning vertical driving device 52, a group of cleaning devices 53 and a liquid pumping pipe 54, wherein the cleaning support frame body 51 is fixedly connected with the top plate inside the outer box 7, the cleaning vertical driving device 52 is arranged on the cleaning support frame body 51, the group of cleaning devices 53 are arranged on the cleaning vertical driving device 52 in a row, the liquid pumping pipe 54 is arranged on the cleaning vertical driving device 52 for connection, one end of the liquid pumping pipe 54 is connected with the microplate centrifugal drying mechanism 6, and the other end of the liquid pumping pipe 54 is connected with a pump. The working principle of cleaning the vertical driving device 52 is the same as that of transferring the vertical driving device 32, when the vertical driving device 52 drives the group of cleaning device 53 and the liquid suction pipe 54 to descend into the box body 72, the cleaning of the elisa plate 300 on the elisa plate centrifugal drying mechanism 6 can be carried out, and after the cleaning is finished, the vertical driving device 52 drives the group of cleaning device 53 and the liquid suction pipe 54 to move out of the box body 72.
Wherein, the vertical cleaning driving device 52 comprises a vertical driving motor 320, two symmetrical linear sliding rails 321, two symmetrical linear guide rails 322, a screw rod supporting seat 323, a vertical driving screw rod 324, a screw rod nut 325, a vertical sliding plate 326, a push arm 327, a push arm 328 and two symmetrical vertical guide posts 329, the vertical driving motor 320 is connected with the vertical driving screw rod 324, the vertical driving screw rod 324 is arranged on the screw rod supporting seat II 323, the two symmetrical linear sliding rails 321, the two symmetrical linear guide rails 322, the screw rod supporting seat 323 and the two symmetrical vertical guide posts 329 are all arranged on the cleaning supporting frame body 51, the linear sliding rails II 321 are provided with a sliding block II 3210, a connecting plate I3211 is connected between the sliding blocks II 3210, the two symmetrical linear guide rails III 322 are provided with a sliding block III 2, a connecting plate II 3213 is connected between the sliding blocks III 2, the two sliding blocks II 325 are in threaded connection with the vertical driving screw rod nut 325, the vertical sliding plate I3213 is fixedly connected with the screw rod nut II 3213, the sliding arm III is connected with the sliding plate 3213, the two sliding arms 3213, the sliding arms are hinged to the two sliding arms 328, the two sliding arms 3213, the two sliding arms are arranged at the ends of the two sliding arms 3213, the two sliding arms are arranged in parallel to the ends, the connecting plate 3213, and the connecting plate 3213, the two sliding arms are arranged at the ends of the two sliding arms 328, and the two sliding arms 3213, the lower end of the scissor mechanism 3214 is connected to a cleaning mounting plate 520, and the set of cleaning devices 53 and the liquid suction pipe 54 are disposed on the cleaning mounting plate 520.
The washing device 53 as shown in fig. 10 and 11 includes a set of washing brush assemblies 531, a brush rotation driving cylinder 532, a cylinder mounting frame 533, a rotation driving rack 534, a set of rotation driving gears 535, and a set of rotation shafts 536, the cylinder mounting frame 533 and the washing mounting plate 520 are fixedly connected, the brush rotation driving cylinder 532 is disposed on the cylinder mounting frame 533, and the brush rotation driving cylinder 532 and the rotation driving rack 534 are connected, the rotation driving rack 534 and the cylinder mounting frame 533 are slidably connected, and the rotation driving rack 534 and one of the set of rotation driving gears 535 are engaged, the set of rotation driving gears 535 are disposed in a line and engaged with each other, the set of washing brush assemblies 535, the set of rotation driving gears 535 and the set of rotation shafts 536 are disposed in one-to-one correspondence, the rotation driving gears 535 and the upper end portions of the rotation shafts 536 are connected, the washing brush assemblies 531 and the rotation shafts 536 are connected, the set of washing brush assemblies 531 of the set of washing device 53 are disposed in a rectangular array, and the set of washing brush assemblies 531 of the set of washing device 53 and plate apertures 531 of the microplate 300 are disposed in one-to-one correspondence. The piston rod of the brush rotation drive cylinder 532 extends or retracts to cause the rotation drive rack 534 to translate, thereby causing the rotation drive gears 535 of the set of rotation drive gears 535 that are engaged with the rotation drive rack 534 to rotate, the set of rotation drive gears 535 are arranged in a row, and the set of rotation drive gears 535 are engaged with each other, so that the set of rotation drive gears 535 rotate synchronously to cause the set of rotation shafts 536 to rotate synchronously.
In the above structure, the cleaning brush assembly 531 includes a band 5311, a vertical connecting plate 5312, a cleaning brush 5313, a brush vertical driving cylinder 5314, a brush mounting frame 5315 and a cleaning nozzle 5316, the band 5311 is connected to the rotating shaft 536, the vertical connecting plate 5312 is connected to the band 5311, the brush vertical driving cylinder 5314 is disposed on the vertical connecting plate 5312, a piston rod of the brush vertical driving cylinder 5314 is connected to the brush mounting frame 5315, the cleaning brush 5313 and the cleaning nozzle 5316 are both disposed on the brush mounting frame 5315, and the cleaning brush 5313 and the cleaning nozzle 5316 extend into a plate hole of the microplate 300. The rotation shaft 536 rotates, so that the brush mounting bracket 5315 rotates around the rotation shaft 536 about the rotation shaft 536 as a central axis, and the cleaning brush 5313 makes a circular motion, so that the cleaning brush 5313 contacts with the inner wall of the plate hole on the microplate 300, thereby cleaning the microplate 300.
The elisa plate centrifugal drying mechanism 6 shown in fig. 12 comprises a centrifugal drying base 61, a centrifugal drying driving motor 62, a centrifugal drying driving wheel 63, a centrifugal drying driven wheel 64, a transmission chain 65, a centrifugal drying rotating shaft 66, a rotating plate 67, two elisa plate placing devices 68 and a drying support frame 69 which are symmetrically arranged, wherein the centrifugal drying base 61 is fixedly arranged on a bottom plate of the elisa plate drying tank body 4, the centrifugal drying driving motor 62 and the drying support frame 69 are fixedly arranged on the centrifugal drying base 61, the centrifugal drying driving motor 62 is connected with the centrifugal drying driving wheel 63, the centrifugal drying driving wheel 63 is connected with the centrifugal drying driven wheel 64 through the transmission chain 65, the centrifugal drying driven wheel 64 is connected with the centrifugal drying rotating shaft 66, the centrifugal drying rotating shaft 66 is connected with the rotating plate 67, two ends of the rotating plate 67 are arranged on the drying support frame 69, and the two elisa plate placing devices 68 which are symmetrically arranged are arranged on the drying support frame 69. The centrifugal spin-drying driving motor 62 rotates to drive the centrifugal spin-drying driving wheel 63 to rotate, the centrifugal spin-drying driving wheel 63 drives the centrifugal spin-drying driven wheel 64 to rotate through the transmission chain 65, and then the centrifugal spin-drying rotating shaft 66 rotates, so that the two elisa plate placing devices 68 symmetrically arranged on the rotating plate 67 are switched, and the elisa plate 300 is dried after being cleaned.
The microplate containing device 68 shown in fig. 13 and 14 comprises a set of microplate compacting assemblies 681, a liquid storage box 682, a rectangular microplate plate supporting frame 683 and two linear modules 684, wherein the two linear modules 684 are arranged on the rotating plate 67, the liquid storage box 682 is fixedly connected with a slider of the two linear modules 684, the set of microplate compacting assemblies 681 are arranged around the rectangular microplate plate supporting frame 683, the rectangular microplate plate supporting frame 683 is fixedly arranged on a bottom plate in the liquid storage box 682, the upper end of the rectangular microplate plate supporting frame 683 extends out of the liquid storage box 682, the set of microplate compacting assemblies 681 compact the peripheral edge of the microplate plate 300 on the rectangular microplate plate supporting frame 683, a gap is arranged between the outer wall of the rectangular microplate plate supporting frame 683 and the inner wall of the liquid storage box 682, one end of the liquid suction pipe 54 is arranged in the gap, and the rectangular microplate plate supporting frame 683 is provided with a plurality of through holes, and the inside of the microplate plate 683 is communicated with the liquid storage box 682 through the plurality of through holes. The liquid storage box 682 receives the cleaning liquid discharged from the cleaning nozzle 5316, and the cleaning liquid flowing into the liquid storage box 682 is continuously drawn out through the liquid suction pipe 54 and recycled, and the microplate rectangular support frame 683 functions to support the microplate 300.
The microplate pressing assembly 681 shown in fig. 13 comprises a pressing driving cylinder 6811, a pressing fixing support 6812, a pressing turning rod 6813 and a pressing head 6814, wherein a piston rod of the pressing driving cylinder 6811 is hinged to one end of the pressing turning rod 6813, the middle position of the pressing turning rod 6813 is connected with the pressing fixing support 6812 through a rotating shaft, the pressing head 6814 is arranged at the end of the pressing turning rod 6813 far away from the pressing driving cylinder 6811, and the pressing head 6814 presses the edge of the periphery of the microplate 300. The piston rod of the pressing driving cylinder 6811 extends out, so that the pressing and overturning rod 6813 overturns, and presses the pressing head 6814 at the position where the microplate 300 is placed on the rectangular supporting frame 683 of the microplate.
The outer box 7 shown in fig. 15 includes a box base 71, a box body 72, and a lifting door 73, wherein the box body 72 is fixedly arranged on the box base 71, one end of the box body 72 close to the microplate reader 200 is provided with a rectangular opening 74, the lifting door 73 is arranged on the box body 72, and the lifting door 73 covers the rectangular opening 74.
As shown in fig. 16, the lifting door 73 includes a lifting door body 731, a lifting driving motor 732, a lifting support frame 733, a lifting driving screw 734 and two symmetrically arranged lifting guide plates 735, wherein the lifting support frame 733 and the two symmetrically arranged lifting guide plates 735 are fixedly arranged on a top plate outside the box body 72, the lifting driving motor 732 and the lifting driving screw 734 are both arranged on the lifting support frame 733, the lifting driving motor 732 is connected with the lifting driving screw 734, the lifting door body 731 and a screw nut of the lifting driving screw 734 are fixedly connected, the section of the lifting door body 731 is rectangular, the lifting door body 731 covers the rectangular opening 74, two sides of the lifting door body 731 are arranged in the two symmetrically arranged lifting guide plates 735, and two sides of the lifting door body 731 are slidably connected with the lifting guide plates 735.
Based on the structure, the working principle of the microplate reader device with the cleaning structure comprises the following steps:
s1, placing the detected ELISA plate 300 back on the ELISA reader 200;
s2, pressing a start button on the cleaning machine 100, receiving an instruction by the cleaning machine 100, and starting cleaning the ELISA plate 300;
s3, the lifting driving motor 732 rotates clockwise to drive the lifting screw 734 to rotate clockwise, so that the lifting screw nut on the driving screw 734 lifts the driving screw 734 along the lifting, the lifting door body 731 lifts, and the rectangular opening 74 is opened;
s4, initially, the elisa plate grabbing manipulator 17 is opposite to the rectangular opening 74, the grabbing reciprocating driving device 12 drives the elisa plate grabbing manipulator 17 to move towards the direction of the rectangular opening 74, the elisa plate grabbing manipulator 17 moves out of the box body 72, and the elisa plate grabbing manipulator 17 moves linearly to a position right above the elisa plate 300 on the elisa plate reader 200;
s5, extending out a piston rod of the grabbing driving cylinder 171, pushing the Y-shaped connecting plate 174 to rotate around the overturning supporting plate 173, and overturning the grabbing overturning plate 175 above the ELISA plate 300;
s6, adsorbing the ELISA plate 300 by a group of ELISA plate suckers 176;
s7, driving the ELIAS plate grabbing manipulator 17 to move towards the box body 72 by the grabbing reciprocating driving device 12, so that the ELIAS plate grabbing manipulator 17 and the ELIAS plate transferring and placing platform 2 are positioned on the same straight line;
s8, the translational linear module 13 drives the ELISA plate grabbing manipulator 17 to move towards the direction of the ELISA plate transferring platform 2 until the ELISA plate 300 adsorbed by a group of ELISA plate suckers I176 is positioned above the ELISA plate transferring platform 2;
s9, releasing the ELISA plate 300 by a group of ELISA plate suckers I176, so that the ELISA plate 300 falls on the ELISA plate transferring platform 2;
s10, driving a group of ELISA plate suckers II 33 to move to the upper part of the ELISA plate transferring and placing platform 2 by a transferring horizontal driving device 31;
s11, driving a group of second enzyme label plate suckers 33 to descend by a transfer vertical driving device 32, and adsorbing the enzyme label plate 300 on the enzyme label plate transfer placing platform 2 by the group of second enzyme label plate suckers 33;
s12, resetting the transfer vertical driving device 32, and driving a group of second enzyme label plate suckers 33 to move right above the first automatic box door 41 by the transfer horizontal driving device 31;
s13, starting a centrifugal drying driving motor 62, driving a rotating plate 67 to turn over by rotating a centrifugal drying driving wheel 63, a centrifugal drying driven wheel 64 and a transmission chain 65, and enabling an ELISA plate placing device 68 above two symmetrically arranged ELISA plate placing devices 68 to be arranged upwards;
s14, the microplate placing device 68 works: the second linear module 684 drives the liquid storage box 682 to move to be opposite to the first automatic box door 41;
s15, opening the first automatic box door 41, driving a group of second enzyme label plate suckers 33 to descend by a transfer vertical driving device 32, enabling the group of second enzyme label plate suckers 33 to descend to the position of a rectangular supporting frame 683 of the enzyme label plate, releasing the enzyme label plate 300 on the rectangular supporting frame 683 by the group of second enzyme label plate suckers 33, and placing the peripheral edge of the enzyme label plate 300 on the upper end face of the rectangular supporting frame 683;
s16, starting a pressing driving cylinder 6811, driving a pressing turning rod 6813 to rotate around a pressing fixed support 6812, so that a pressing head 6814 is pressed on the peripheral edge of the ELISA plate 300, and the ELISA plate 300 is fixed on a rectangular support frame 683;
s17, the ELISA plate placing device 68 works: the second linear module 684 drives the liquid storage box 682 in which the elisa plate 300 is placed to move to be opposite to the second automatic box door 42;
s18, opening the second automatic box door 42, and closing the first automatic box door 41;
s19, driving a group of cleaning devices 53 and a liquid extracting pipe 54 to descend by a cleaning vertical driving device 52 until the liquid extracting pipe 54 extends between a rectangular supporting frame 683 of the ELISA plate and a liquid storage box 682;
s20, the brush vertical driving cylinder 5314 continues to drive the cleaning brush 5313 and the cleaning nozzle 5316 to descend until the cleaning brush 5313 and the cleaning nozzle 5316 extend into the plate hole of the ELISA plate 300;
s21, starting a cleaning nozzle 5316 to spray cleaning liquid into the plate hole of the ELISA plate 300;
s22, a piston rod of the brush rotation driving cylinder 532 extends out to drive the rotation driving rack 534 to move, the rotation driving rack 534 drives the rotation driving gear 535 meshed with the rotation driving rack 534 to rotate, and the group of rotation driving gears 535 rotate and the group of rotation shafts 536 rotate due to the meshing of the group of rotation driving gears 535;
s23, rotating the brush mounting frame 5315 around the rotating shaft 536, so that the cleaning brush 5313 rotates in the plate hole of the ELISA plate 300 by taking the rotating shaft 536 as a central axis, and the cleaning brush 5313 contacts with the inner wall of the plate hole of the ELISA plate 300 in the rotating process, so that the plate hole of the ELISA plate 300 is cleaned;
s24, the piston rod of the brush vertical driving cylinder 5314 extends out or retracts to enable the cleaning brush 5313 to be located at different depth positions in the plate hole of the ELISA plate 300;
s25, continuously spraying cleaning liquid into the plate holes of the ELISA plate 300 by a cleaning nozzle 5316, enabling the cleaning liquid in the plate holes of the ELISA plate 300 to overflow the plate holes of the ELISA plate 300, enabling the cleaning liquid to flow into the liquid storage box 682, and continuously extracting the cleaning liquid in the liquid storage box 682 by a liquid extracting pipe 54 for recovery;
s26, after the washing is finished, the brush vertically drives the air cylinder 5314 to reset, and the washing vertical driving device 52 resets, so that the washing brush 5313 and the washing nozzle 5316 leave the plate holes of the ELISA plate 300;
s27, starting a centrifugal drying driving motor 62, and driving a rotating plate 67 to turn over by rotating a centrifugal drying driving wheel 63, a centrifugal drying driven wheel 64 and a transmission chain 65 so that the other elisa plate placing device 68 faces upwards;
s28, repeating the steps S14 to S26, and cleaning the ELIAS plate 300 on the two symmetrically-arranged ELIAS plate placing devices 68;
s29, closing the second automatic box door 42 and the first automatic box door 41;
s30, starting a centrifugal drying driving motor 62 to drive a rotating plate 67 to rotate at a high speed, so that the ELISA plates 300 on two symmetrically-arranged ELISA plate placing devices 68 are dried;
s31, after the enzyme label plate 300 is dried, the second automatic box door 42 is kept closed, and the first automatic box door 41 is opened;
s32, the ELIAS plate transfer mechanism 3 firstly takes down an ELIAS plate 300 from the ELIAS plate centrifugal drying mechanism 6, and the ELIAS plate transfer mechanism 3 places the ELIAS plate 300 on the ELIAS plate transfer placing platform 2;
s33, the ELISA plate grabbing mechanism 1 grabs the ELISA plate 300 on the ELISA plate transferring and placing platform 2 and places the ELISA plate 300 back on the ELISA reader 200;
s34, repeating the steps S32-S33, and placing another ELISA plate 300 back to the ELISA reader 200;
and S35, closing the lifting door 73.
In the elisa plate 300 cleaning and spin-drying process, if only one elisa plate 300 is provided, only one elisa plate 300 is placed on the elisa plate centrifugal spin-drying mechanism 6 for cleaning and spin-drying.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that modifications can be made by those skilled in the art without departing from the principle of the present invention, and these modifications should also be construed as the protection scope of the present invention.
Claims (5)
1. A microplate reader device with a cleaning structure, characterized in that: the ELISA plate cleaning device comprises a cleaning machine (100), an ELISA reader (200) and an ELISA plate (300), wherein the cleaning machine (100) is positioned on one side of the ELISA reader (200), the ELISA plate (300) is arranged on the ELISA reader (200), and the cleaning machine (100) cleans the ELISA plate (300);
the washing machine (100) comprises an ELISA plate grabbing mechanism (1), an ELISA plate transferring and placing platform (2), an ELISA plate transferring mechanism (3), an ELISA plate spin-drying tank body (4), an ELISA plate washing mechanism (5), an ELISA plate centrifugal spin-drying mechanism (6) and an outer tank body (7), wherein the ELISA plate grabbing mechanism (1), the ELISA plate transferring and placing platform (2), the ELISA plate transferring mechanism (3), the ELISA plate spin-drying tank body (4) and the ELISA plate washing mechanism (5) are all arranged in the outer tank body (7), and the ELISA plate centrifugal spin-drying mechanism (6) is arranged in the ELISA plate spin-drying tank body (4);
the elisa plate grabbing mechanism (1) transports the elisa plate (300) between the elisa plate reader (200) and the elisa plate transporting and placing platform (2);
the elisa plate transferring mechanism (3) transfers the elisa plate (300) between the elisa plate transferring and placing platform (2) and the elisa plate centrifugal drying mechanism (6);
the ELISA plate cleaning mechanism (5) is used for cleaning the ELISA plate (300) on the ELISA plate centrifugal drying mechanism (6);
the ELISA plate centrifugal drying mechanism (6) is used for performing centrifugal drying on the ELISA plate (300);
the ELISA plate cleaning mechanism (5) comprises a cleaning support frame body (51), a cleaning vertical driving device (52), a cleaning device (53) and a liquid pumping pipe (54), the cleaning support frame body (51) is fixedly connected with a top plate inside the outer box body (7), the cleaning vertical driving device (52) is arranged on the cleaning support frame body (51), the cleaning device (53) is arranged on the cleaning vertical driving device (52), the liquid pumping pipe (54) is connected with the cleaning vertical driving device (52), one end of the liquid pumping pipe (54) is connected with the ELISA plate centrifugal drying mechanism (6), and the other end of the liquid pumping pipe (54) is connected with a pump;
the cleaning vertical driving device (52) comprises a vertical driving motor (320), two symmetrically arranged linear sliding rails II (321), two symmetrically arranged linear guide rails III (322), a screw rod supporting seat II (323), a vertical driving screw rod (324), a screw rod nut II (325), a vertical sliding plate I (326), a push arm I (327), a push arm II (328) and two symmetrically arranged vertical guide posts II (329), wherein the vertical driving motor (320) is connected with the vertical driving screw rod (324), the vertical driving screw rod (324) is arranged on the screw rod supporting seat II (323), the two symmetrically arranged linear sliding rails II (321), the two symmetrically arranged linear guide rails III (322), the screw rod supporting seat II (323) and the two symmetrically arranged vertical guide posts II (329) are all arranged on a cleaning supporting frame body (51), a sliding block II (3210) is arranged on the linear sliding rail II (321), a sliding block I (3211) is connected between the sliding block II (3210), a sliding block III (3212) is arranged on the two symmetrically arranged linear guide rails III (322), and the vertical screw rod nut II (325) is connected with the vertical driving screw rod nut connecting plate (325), the cleaning device comprises a first push arm (327) and a second push arm (328), wherein the first push arm (327) and the second push arm (328) are arranged in a V shape, the position of the intersection point of the first push arm (327) and the second push arm (328) is hinged to a second screw nut (325), the end, far away from the second screw nut (325), of the first push arm (327) is hinged to a first connecting plate (3211), the end, far away from the second screw nut (325), of the second push arm (328) is hinged to a second connecting plate (3213), the two symmetrically arranged vertical guide columns (329) and the vertical driving screw rod (324) are arranged in parallel, the vertical driving screw rod (324) is located between the two symmetrically arranged vertical guide columns (329), the first vertical sliding plate (326) is connected with the two symmetrically arranged vertical guide columns (329) in a sliding manner, a scissor mechanism (3214) is connected between the first connecting plate (3211) and the second connecting plate (3213), the lower end part of the scissor mechanism (3214) is connected with a cleaning mounting plate (520), and the cleaning device (53) and the cleaning device (54) are arranged on the mounting plate (520);
the cleaning device (53) comprises a group of cleaning brush assemblies (531), a brush rotation driving cylinder (532), a cylinder mounting frame body (533), a rotation driving rack (534), a group of rotation driving gears (535) and a group of rotation shafts (536), wherein the cylinder mounting frame body (533) is fixedly connected with the cleaning mounting plate (520), the brush rotation driving cylinder (532) is arranged on the cylinder mounting frame body (533), the brush rotation driving cylinder (532) is connected with the rotation driving rack (534), the rotation driving rack (534) is slidably connected with the cylinder mounting frame body (533), the rotation driving rack (534) is meshed with the rotation driving gears (535), the group of rotation driving gears (535) are arranged in a row and meshed with the group of rotation driving gears (535), the cleaning brush assemblies (531), the rotation driving gears (535) and the rotation shafts (536) are arranged in a one-to-one correspondence manner, the rotation driving gears (535) are connected with the upper end portions of the rotation shafts (536), the cleaning brush assemblies (531) are connected with the rotation shafts (536), the group of cleaning brush assemblies (53) are arranged in a one-to-one manner, and the cleaning brush assemblies (531) are arranged in a rectangular plate hole array manner, and the cleaning brush assemblies (300) are arranged in one-to-one;
the cleaning brush component (531) comprises a hoop (5311), a vertical connecting plate (5312), a cleaning brush (5313), a brush vertical driving cylinder (5314), a brush mounting frame (5315) and a cleaning nozzle (5316), the hoop (5311) is connected with a rotating shaft (536), the vertical connecting plate (5312) is connected with the hoop (5311), the brush vertical driving cylinder (5314) is arranged on the vertical connecting plate (5312), a piston rod of the brush vertical driving cylinder (5314) is connected with the brush mounting frame (5315), the cleaning brush (5313) and the cleaning nozzle (5316) are both arranged on the brush mounting frame (5315), and the cleaning brush (5313) and the cleaning nozzle (5316) extend into plate holes of the enzyme label plate (300);
the ELISA plate centrifugal drying mechanism (6) comprises a centrifugal drying base (61), a centrifugal drying driving motor (62), a centrifugal drying driving wheel (63), a centrifugal drying driven wheel (64), a transmission chain (65), a centrifugal drying rotating shaft (66), a rotating plate (67), two ELISA plate placing devices (68) and a drying support frame (69) which are symmetrically arranged, wherein the centrifugal drying base (61) is fixedly arranged on a bottom plate of an ELISA plate drying tank body (4), the centrifugal drying driving motor (62) and the drying support frame (69) are fixedly arranged on the centrifugal drying base (61), the centrifugal drying driving motor (62) is connected with the centrifugal drying driving wheel (63), the centrifugal drying driving wheel (63) is connected with the centrifugal drying driven wheel (64) through the transmission chain (65), the centrifugal drying driven wheel (64) is connected with the centrifugal rotating shaft (66), the centrifugal drying rotating shaft (66) is connected with the rotating plate (67), two ends of the rotating plate (67) are arranged on the drying support frame (69), and the two ELISA plate placing devices (68) which are symmetrically arranged are arranged on the rotating plate (67);
the ELISA plate placing device (68) comprises a group of ELISA plate pressing assemblies (681), a liquid storage box (682), an ELISA plate rectangular supporting frame (683) and two linear modules (684), wherein the two linear modules (684) are arranged on a rotary plate (67), sliders of the liquid storage box (682) and the two linear modules (684) are fixedly connected, the group of ELISA plate pressing assemblies (681) are arranged on the periphery of the ELISA plate rectangular supporting frame (683), the ELISA plate rectangular supporting frame (683) is fixedly arranged on an inner bottom plate of the liquid storage box (682), the upper end part of the ELISA plate rectangular supporting frame (683) extends out of the liquid storage box (682), the peripheral edges of the ELISA plate (300) are pressed on the ELISA plate rectangular supporting frame (683) by the group of ELISA plate pressing assemblies (681), a gap is formed between the outer wall of the ELISA plate rectangular supporting frame (683) and the inner wall of the ELISA plate rectangular supporting frame (682), one end of a liquid pumping pipe (54) is arranged in the gap, and a plurality of ELISA plate rectangular supporting frames (683) are communicated with a plurality of through holes formed in the ELISA plate rectangular supporting frame (683).
2. The microplate reader device of claim 1, wherein: the ELISA plate grabbing mechanism (1) comprises a grabbing support base (11), a grabbing reciprocating driving device (12), a translation linear module (13), a translation linear guide rail (14), a translation linear sliding block (15), a Z-shaped support plate (16) and an ELISA plate grabbing manipulator (17), the grabbing support base (11) is fixedly arranged on a bottom plate inside an outer box body (7), the grabbing reciprocating driving device (12) is arranged on the grabbing support base (11), the translation linear module (13) and the translation linear guide rail (14) are arranged on the grabbing reciprocating driving device (12), the translation linear guide rail (14) and the translation linear module (13) are arranged in parallel in the moving direction, the translation linear sliding block (15) is connected with the translation linear guide rail (14) in a sliding mode, one end of the Z-shaped support plate (16) is connected with the sliding block and the translation linear sliding block (15) of the translation linear module (13), the ELISA plate grabbing manipulator (17) is arranged at the other end of the Z-shaped support plate (16), and the ELISA plate grabbing manipulator (17) is arranged between an ELISA plate placing platform (200) and an ELISA plate placing transport platform (2).
3. The microplate reader device with a cleaning structure of claim 1, wherein: ELIAS plate transport mechanism (3) are including transporting horizontal drive device (31), transporting vertical drive arrangement (32) and a set of ELIAS plate sucking disc two (33), transport horizontal drive arrangement (31) and set up on the inside roof of outer box (7), it connects with transporting horizontal drive arrangement (31) to transport vertical drive arrangement (32), a set of ELIAS plate sucking disc two (33) and transport vertical drive arrangement (32) and connect, it transports place the platform (2) and the ELIAS plate jar body (4) top to be located the ELIAS plate respectively to transport horizontal drive arrangement (31) moving direction's both ends.
4. The microplate reader device of claim 3, wherein: the transfer vertical driving device (32) comprises a vertical driving motor (320), two symmetrically arranged linear sliding rails II (321), two symmetrically arranged linear guiding rails III (322), a screw rod supporting seat II (323), a vertical driving screw rod (324), a screw rod nut II (325), a vertical sliding plate I (326), a push arm I (327), a push arm II (328) and two symmetrically arranged vertical guide posts II (329), wherein the vertical driving motor (320) is connected with the vertical driving screw rod (324), the vertical driving screw rod (324) is arranged on the screw rod supporting seat II (323), the two symmetrically arranged linear sliding rails II (321), the two symmetrically arranged linear guiding rails III (322), the screw rod supporting seat II (323) and the two symmetrically arranged vertical guide posts II (329) are all arranged on the supporting frame (317), a sliding block II (3210) is arranged on the linear sliding rails II (321), a connecting plate I (3211) is connected between the sliding blocks II (3210), the two symmetrically arranged linear guiding rails III (322) are provided with the sliding blocks III (322), a sliding block II (3213) is connected between the sliding plates III (3212), and the vertical driving screw rod nut II (325) is connected with the vertical driving screw rod nut connecting plate 325), the device comprises a first push arm (327) and a second push arm (328) which are arranged in a V shape, wherein the cross point of the first push arm (327) and the second push arm (328) is hinged to a second screw nut (325), the end, far away from the second screw nut (325), of the first push arm (327) is hinged to a first connecting plate (3211), the end, far away from the second screw nut (325), of the second push arm (328) is hinged to a second connecting plate (3213), the two symmetrically arranged vertical guide posts (329) and the vertical driving screw (324) are arranged in parallel, the vertical driving screw (324) is located between the two symmetrically arranged vertical guide posts (329), the first vertical sliding plate (326) is connected with the two symmetrically arranged vertical guide posts (329) in a sliding manner, a shearing fork mechanism (3214) is connected between the first connecting plate (3211) and the second connecting plate (3213), the lower end of the shearing fork mechanism (3214) is connected with a suction cup (3215), a set of two elisa plates (33) is arranged on the suction cup mounting plate (3215), and a set of two elisa plates (300) is arranged on the suction plate.
5. A microplate cleaning method for a microplate reader device having a cleaning structure according to claim 1, characterized in that: the method comprises the following steps:
s1, pushing the elisa plate (300) out of the elisa plate reader (200) after the test by the elisa plate reader (200);
s2, automatically opening a box door of the outer box body (7);
s3, the elisa plate grabbing mechanism (1) extends out of the outer box body (7), and the elisa plate grabbing mechanism (1) grabs the elisa plate (300) on the elisa plate reader (200);
s4, retracting the ELISA plate grabbing mechanism (1) into the outer box body (7);
s5, placing the ELISA plate (300) on an ELISA plate transferring and placing platform (2) by the ELISA plate grabbing mechanism (1);
s6, moving the ELISA plate (300) on the ELISA plate transferring and placing platform (2) to an ELISA plate spin-drying tank body (4) by the ELISA plate transferring mechanism (3), and placing the ELISA plate (300) in the ELISA plate spin-drying tank body (4) by the ELISA plate transferring mechanism (3);
s7, cleaning the enzyme label plate (300) in the enzyme label plate drying tank body (4) by the enzyme label plate cleaning mechanism (5);
s8, after the ELISA plate (300) is cleaned, an automatic door on the ELISA plate spin-drying tank body (4) is closed, and the ELISA plate spin-drying tank body (4) carries out centrifugal spin-drying on the ELISA plate (300);
s9, taking out the centrifugally-dried ELISA plate (300) by the ELISA plate transferring mechanism (3), and transferring the ELISA plate (300) to an ELISA plate transferring and placing platform (2);
s10, an ELISA plate grabbing mechanism (1) grabs and moves out of an outer box body (7) an ELISA plate (300) on an ELISA plate transferring and placing platform (2);
s11, placing the ELISA plate (300) on the ELISA reader (200) by the ELISA plate grabbing mechanism (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211125239.2A CN115193789B (en) | 2022-09-16 | 2022-09-16 | Elisa plate cleaning method and elisa instrument equipment with cleaning structure |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211125239.2A CN115193789B (en) | 2022-09-16 | 2022-09-16 | Elisa plate cleaning method and elisa instrument equipment with cleaning structure |
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| Publication Number | Publication Date |
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| CN115193789A CN115193789A (en) | 2022-10-18 |
| CN115193789B true CN115193789B (en) | 2023-03-24 |
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| CN202211125239.2A Active CN115193789B (en) | 2022-09-16 | 2022-09-16 | Elisa plate cleaning method and elisa instrument equipment with cleaning structure |
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| JP4467367B2 (en) * | 2004-06-22 | 2010-05-26 | 大日本スクリーン製造株式会社 | Substrate reversing device, substrate transporting device, substrate processing device, substrate reversing method, substrate transporting method, and substrate processing method |
| JP5422143B2 (en) * | 2008-06-04 | 2014-02-19 | 株式会社荏原製作所 | Substrate gripping mechanism |
| JP7072415B2 (en) * | 2018-03-26 | 2022-05-20 | 株式会社Ｓｃｒｅｅｎホールディングス | Board processing method and board processing equipment |
| CN108787531A (en) * | 2018-08-07 | 2018-11-13 | 陈亚元 | A kind of fully-automatic intelligent brush plate machine |
| CN213349851U (en) * | 2020-08-14 | 2021-06-04 | 太仓市多多过滤材料有限公司 | Micropore plate washs and air-dries integrative device |
| CN114101203B (en) * | 2021-11-22 | 2022-09-30 | 苏州康容生物医疗科技有限公司 | Full-automatic enzyme label plate washing machine and using method thereof |
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