CN115193595A - Exosome filtering and extracting device and exosome filtering and extracting method - Google Patents
Exosome filtering and extracting device and exosome filtering and extracting method Download PDFInfo
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- CN115193595A CN115193595A CN202210851071.7A CN202210851071A CN115193595A CN 115193595 A CN115193595 A CN 115193595A CN 202210851071 A CN202210851071 A CN 202210851071A CN 115193595 A CN115193595 A CN 115193595A
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 44
- 238000001914 filtration Methods 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000012360 testing method Methods 0.000 claims abstract description 69
- 230000007246 mechanism Effects 0.000 claims abstract description 49
- 238000000605 extraction Methods 0.000 claims abstract description 13
- 238000005119 centrifugation Methods 0.000 claims abstract description 8
- 230000005540 biological transmission Effects 0.000 claims description 14
- 230000008569 process Effects 0.000 claims description 14
- 238000001179 sorption measurement Methods 0.000 claims description 10
- 239000012472 biological sample Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 4
- 238000013459 approach Methods 0.000 claims description 3
- 238000011084 recovery Methods 0.000 claims description 3
- 230000010355 oscillation Effects 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 5
- 239000011435 rock Substances 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 4
- 235000017491 Bambusa tulda Nutrition 0.000 description 4
- 241001330002 Bambuseae Species 0.000 description 4
- 241000252254 Catostomidae Species 0.000 description 4
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 4
- 239000011425 bamboo Substances 0.000 description 4
- 238000000703 high-speed centrifugation Methods 0.000 description 4
- 239000012528 membrane Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000030741 antigen processing and presentation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000035992 intercellular communication Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 208000037816 tissue injury Diseases 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0269—Solid material in other moving receptacles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B13/00—Control arrangements specially designed for centrifuges; Programme control of centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Centrifugal Separators (AREA)
Abstract
The invention discloses an exosome filtering and extracting device and an exosome filtering and extracting method, and relates to the technical field of exosome extraction, wherein the extracting device comprises a centrifugal box body, a positioning disc and a driving disc, wherein the positioning disc and the driving disc are arranged in the centrifugal box body; the test tube positioning device comprises a positioning cylinder, a clamping mechanism and a positioning mechanism, wherein the positioning cylinder is internally distributed with the clamping mechanism, and the clamping mechanism is used for clamping and fixing the test tube placed in the positioning cylinder; the test tube fixing device further fixes the test tube by arranging the clamping mechanism in the positioning cylinder, thereby improving the stability of the test tube in the centrifugation process and avoiding the phenomena of shaking, oscillation and offset.
Description
Technical Field
The invention relates to the technical field of exosome extraction, in particular to an exosome filtering and extracting device and an exosome extracting method.
Background
Exosome is a small membrane vesicle containing complex RNA and protein, is regarded as a specific secreted membrane vesicle at present and participates in intercellular communication, and mainly comprises 3 major components, namely protein, nucleic acid and lipid, and plays an important role in the physiological and pathological aspects of antigen presentation in immunity, tumor growth and migration, tissue injury repair and the like;
in the extraction of exosomes, the ultracentrifugation method for separating exosomes is the most common method at present, and the method is widely applied to the analysis of various biological samples, such as serum, plasma, cell culture fluid, urine, saliva, cerebrospinal fluid and the like, and is also the 'gold standard' in the current separation method;
to treat centrifugal biological sample at first arranges the test tube in, then place the centrifuge with the test tube and carry out centrifugal processing, but, to current centrifugal device, set up a plurality of standing grooves that are used for advancing line location to the test tube usually, consequently when the test tube diameter is great, can not put into the standing groove with the test tube and advance line location, also adopted the standing groove that sets up great internal diameter, but when advancing line location to the test tube that is less than the standing groove internal diameter, rock and vibrate very easily under high-speed centrifugation.
Disclosure of Invention
The invention aims to provide an exosome filtering and extracting device and an exosome filtering and extracting method, and the exosome filtering and extracting device and the exosome extracting method solve the following technical problems:
when the test tube diameter is great, can not put into the standing groove with the test tube and fix a position, also adopt the standing groove that sets up great internal diameter, but when fixing a position the test tube that is less than the standing groove internal diameter, rock and vibrate under high-speed centrifugation very easily.
The purpose of the invention can be realized by the following technical scheme:
a exosome filtering and extracting device comprises a centrifugal box body, a positioning disc and a driving disc, wherein the positioning disc and the driving disc are arranged in the centrifugal box body;
the test tube positioning device comprises a positioning cylinder, a clamping mechanism and a positioning mechanism, wherein the positioning cylinder is internally distributed with the clamping mechanism, and the clamping mechanism is used for clamping and fixing the test tube placed in the positioning cylinder;
the centrifugal box is characterized by further comprising a first motor arranged at the bottom of the centrifugal box body, a driving cylinder is fixedly installed below the driving disc, and the output end of the first motor is fixedly connected with the driving cylinder.
Preferably, the clamping mechanism comprises a plurality of groups of clamping seats distributed in the positioning barrel in a circumferential array, the clamping seats are fixedly connected with positioning rods distributed in the periphery of the positioning barrel in a sliding manner, and the positioning rods are connected with a pushing mechanism which drives the positioning rods to approach or keep away from the axis of the positioning barrel.
Preferably, the pushing mechanism comprises a plurality of groups of turntables rotatably arranged at the bottoms of the driving discs, a plurality of groups of arc-shaped grooves are formed in the surfaces of the turntables in a circumferential array, the arc-shaped grooves extend from the near center end close to the turntables to the far center end, pin shafts are movably embedded in the arc-shaped grooves, a plurality of groups of driving grooves are formed in the driving discs, the pin shafts penetrate through the driving grooves and are fixedly connected with the positioning rods, and reset springs are arranged outside the positioning rods;
the carousel bottom sets up ring gear, each group ring gear and the master gear fixed connection who sets up in the driving-disc bottom of rotating, the master gear with rotate the dwang fixed connection who sets up between positioning disk and driving-disc, the terminal fixed mounting handle of dwang.
Preferably, fixture still includes adsorption apparatus, adsorption apparatus sets up the piston including being the cylinder body that circumference array laid between positioning disk and driving-disc in the cylinder body, and the piston separates the cylinder body and forms first cavity and second cavity, piston and the expanding spring fixed connection who lays in the cylinder body, first pipeline is connected to the second cavity, location bobbin base portion is provided with the absorption hole of being connected with first pipeline.
Preferably, a cavity is arranged in the side wall of the positioning cylinder, the first cavity is communicated with the cavity through a second pipeline, a groove is formed in the clamping seat, an anti-skid pad is embedded in the groove, an air cavity is formed in the positioning rod, an air plug fixedly connected with the anti-skid pad is arranged in the air cavity in a sliding mode, and the air cavity is connected with the cavity through a third pipeline;
preferably, a feeding mechanism is further arranged on one side of the centrifugal box body, the feeding mechanism comprises a plurality of groups of vacuum suckers distributed above the centrifugal box body, the vacuum suckers are connected with a negative pressure cavity through a fourth pipeline, and the negative pressure cavity is fixedly connected with a vacuum pump distributed on the lifting plate through a fifth pipeline;
wherein the lifting plate is connected with the lifting mechanism; each group of the fourth pipelines is positioned and fixed by a cross plate.
Preferably, the lifting mechanism comprises an L-shaped support plate fixedly arranged on the side wall of the centrifugal box body, the lifting box body is fixedly arranged on one side of the L-shaped support plate, a screw rod is arranged in the lifting box body in a rotating mode, a nut fixedly connected with the lifting plate is arranged on the screw rod in a threaded mode, and the screw rod is fixedly connected with a second motor arranged on the lifting box body.
Preferably, the bottom of the cross plate is provided with an infrared detector, and the handle is correspondingly provided with a detector for receiving infrared signals.
Preferably, the lifting device is also provided with a controller module, a transmission module and an operation module, wherein the controller module is used for receiving signals, analyzing and outputting commands, the transmission module is used for transmitting signals and commands, and the operation module is used for receiving commands and controlling the operation of the lifting mechanism;
the extraction method of the exosome filtering and extracting device comprises the following steps:
the method comprises the following steps that firstly, a vacuum pump is started, a test tube is adsorbed and fixed through a vacuum chuck, an infrared detector emits a first signal, the detector receives the first signal and then measures the distance between the test tube and the infrared detector, when the distance R is detected, a transmission module is used for sending the received first signal to a controller module, the controller module is used for executing a first command, and an operation module receives the first command and starts a second motor to operate to drive the test tube to move towards a positioning groove;
the infrared detector emits a second signal, the detector receives the second signal and then measures the distance between the infrared detector and the controller, when the distance is detected to be R, the transmission module is used for sending the received second signal to the controller module, the controller module is used for executing a second command, and the operation module receives the second command to stop the operation of the second motor and the vacuum pump;
step two; the handle is rotated, the main gear is driven by the handle through a rotating rod to rotate, the main gear drives the rotary table to rotate through the ring gear, the pin shaft moves in the arc-shaped groove from the circle center end to the direction close to the center end in the rotating process of the rotary table, and the pin shaft drives the clamping seat to move towards the axis direction through the positioning rod to clamp and fix the test tube;
step three, starting a first motor, wherein the output end of the first motor drives a driving disc to rotate in the rotating process, and the driving disc drives the test tube in the positioning cylinder to carry out centrifugation in the rotating process;
and step four, after the centrifugation is finished, dividing the biological sample into supernatant and exosomes in the test tube, enabling the supernatant to flow into a recovery tube, enabling the exosomes to flow into an exosome storage tube for storage, and completing the separation and extraction.
The invention has the beneficial effects that:
(1) The test tube is arranged in the positioning cylinder after penetrating through the positioning groove, then the test tube is clamped and fixed through the clamping mechanism arranged in the positioning cylinder, the first motor is started, the output end of the first motor drives the driving disc to rotate in the rotating process, and the driving disc drives the test tube in the positioning cylinder to carry out centrifugation in the rotating process;
(2) Under the effect of centrifugal force, the piston moves towards the direction of keeping away from driving-disc centre of a circle end in the cylinder body, and at the in-process that removes, the second cavity is in negative pressure state, and then forms the negative pressure end in adsorption hole department through first pipeline, tightly adsorbs the test tube in a location section of thick bamboo under the atmospheric pressure effect, and then avoids the test tube to rock and vibrate in a location section of thick bamboo when high-speed centrifugation, and stability is higher.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic structural diagram I of an exosome filtering and extracting device according to the present invention;
FIG. 2 is a schematic structural diagram II of an exosome filtration-extraction device of the present invention;
FIG. 3 is a schematic structural diagram of a driving disk in an exosome-filtering extraction device according to the present invention;
FIG. 4 is an enlarged schematic view of the exosome-filtering extraction device of the present invention at A in FIG. 3;
FIG. 5 is a schematic structural diagram of a driving cylinder in the exosome filtering and extracting device of the present invention;
FIG. 6 is a schematic structural diagram of a main gear in an exosome filtering and extracting device according to the present invention;
FIG. 7 is a schematic structural diagram of a cylinder in the exosome filtration-extraction device of the present invention;
FIG. 8 is a schematic structural diagram of a positioning rod in the exosome filtering and extracting device of the present invention.
In the figure: 1. a centrifugal box body; 2. a sealing plate; 3. a first motor; 4. rotating the rod; 5. a detector; 6. a vacuum pump; 7. lifting the box body; 8. positioning a plate; 9. a cylinder body; 101. an L-shaped support plate; 201. a through hole; 301. a drive cylinder; 401. a handle; 501. an infrared detector; 601. a fifth pipeline; 701. a second motor; 702. a nut; 703. a lifting plate; 704. a cross plate; 705. a negative pressure chamber; 706. a screw; 707. a fourth conduit; 708. a vacuum chuck; 801. a drive plate; 802. a positioning cylinder; 803. positioning a groove; 804. positioning a rod; 805. a return spring; 806. a drive slot; 807. a pin shaft; 808. a ring gear; 809. a turntable; 810. an arc-shaped slot; 811. a main gear; 901. a second conduit; 902. a piston; 903. a tension spring; 904. a first pipe; 905. a cavity; 906. a third pipeline; 907. an air cavity; 908. an air lock; 909. a clamping seat; 910. a non-slip mat; 911. a first chamber; 912. a second chamber.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1-3, the present invention is an exosome filtering and extracting device, including a centrifugal box 1, a positioning plate 8 and a driving plate 801 arranged in the centrifugal box 1, wherein a plurality of positioning cylinders 802 for positioning test tubes are arranged between the positioning plate 8 and the driving plate 801, and a plurality of positioning grooves 803 communicated with the positioning cylinders 802 are arranged on the positioning plate 8;
a clamping mechanism is arranged in the positioning cylinder 802 and used for clamping and fixing the test tube placed in the positioning cylinder 802;
the centrifugal box body is characterized by further comprising a first motor 3 arranged at the bottom of the centrifugal box body 1, a driving cylinder 301 is fixedly installed below the driving disc 801, and the output end of the first motor 3 is fixedly connected with the driving cylinder 301;
specifically, in this embodiment, when a biological sample in a test tube is centrifuged, the test tube passes through the positioning groove 803 and is placed in the positioning cylinder 802, and then the test tube is clamped and fixed by the clamping mechanism arranged in the positioning cylinder 802, the first motor 3 is started, the output end of the first motor 3 drives the driving disc 801 to rotate in the rotating process, and the driving disc 801 drives the test tube in the positioning cylinder 802 to be centrifuged.
Referring to fig. 4, as a further solution of the embodiment of the present invention, the clamping mechanism includes a plurality of sets of clamping seats 909 arranged in a circumferential array in the positioning cylinder 802, the clamping seats 909 are fixedly connected with the positioning rod 804 arranged in a sliding manner around the positioning cylinder 802, and the positioning rod 804 is connected with a pushing mechanism driving the positioning rod 804 to approach or depart from the axis of the positioning cylinder 802;
specifically, in this embodiment, before the test tube is placed into the positioning cylinder 802, the pushing mechanism drives the holder 909 to move towards the direction away from the axis center, and then the test tube is placed in the positioning cylinder 802, the pushing mechanism drives the holder 909 to move towards the axis center, so that the test tube is held and fixed by the holder 909, thereby facilitating the assembly and disassembly.
Referring to fig. 5, as a further solution of the embodiment of the present invention, the pushing mechanism includes a plurality of groups of rotating discs 809 rotatably disposed at the bottom of the driving disc 801, a plurality of groups of arc-shaped grooves 810 are circumferentially arranged on the surface of the rotating discs 809, the arc-shaped grooves 810 extend from a proximal end close to the rotating discs 809 to a distal end, a pin shaft 807 is movably embedded in the arc-shaped grooves 810, the driving disc 801 is provided with a plurality of groups of driving grooves 806, the pin shaft 807 penetrates through the driving grooves 806 and is fixedly connected to the positioning rod 804, and a return spring 805 is disposed outside the positioning rod 804;
wherein, the bottom of the turntable 809 is provided with a ring gear 808, each group of the ring gears 808 is fixedly connected with a main gear 811 which is rotatably arranged at the bottom of the driving disk 801, the main gear 811 is fixedly connected with a rotating rod 4 which is rotatably arranged between the positioning disk 8 and the driving disk 801, and the tail end of the rotating rod 4 is fixedly provided with a handle 401;
specifically, in this embodiment, by rotating the handle 401, the handle 401 drives the main gear 811 to rotate through the rotating rod 4, the main gear 811 drives the turntable 809 to rotate through the ring gear 808, the turntable 809 moves in a rotating process so that the pin shaft 807 moves from a center end to a proximal end in the arc-shaped groove 810, the pin shaft 807 drives the grip holder 909 to move towards an axis direction through the positioning rod 804 to clamp and fix the test tube, at this time, the return spring 805 is in a compressed state to generate an elastic force, when the test tube is disassembled, the handle 401 is rotated reversely so that the turntable 809 rotates reversely, and then the pin shaft 807 is pushed to reset under the elastic force of the return spring 805, and the pin shaft 807 drives the grip holder 909 to move towards a direction away from the axis direction through the positioning rod 804 to disassemble the test tube.
Referring to fig. 6, as a further scheme of the embodiment of the present invention, the clamping mechanism further includes an adsorption mechanism, the adsorption mechanism includes cylinder blocks 9 disposed between the positioning plate 8 and the driving plate 801 in a circumferential array, a piston 902 is disposed in the cylinder block 9, the piston 902 separates the cylinder block 9 to form a first chamber 911 and a second chamber 912, the piston 902 is fixedly connected to a telescopic spring 903 disposed in the cylinder block 9, the second chamber 912 is connected to the first pipe 904, and an adsorption hole connected to the first pipe 904 is disposed at the bottom of the positioning cylinder 802;
specifically, in this embodiment, when carrying out the centrifugation, under the effect of centrifugal force, piston 902 removes towards the direction of keeping away from driving-disc 801 centre of a circle end in cylinder 9, at the in-process that removes, second cavity 912 is in the negative pressure state, and then forms the negative pressure end in adsorption hole department through first pipeline 904, tightly adsorbs the test tube in a location section of thick bamboo 802 under the atmospheric pressure effect, and then avoids the test tube to rock and vibrate in a location section of thick bamboo 802 when high-speed centrifugation, and stability is higher.
Referring to fig. 8, as a further solution of the embodiment of the present invention, a cavity 905 is disposed in a side wall of the positioning cylinder 802, the first cavity 911 is communicated with the cavity 905 through a second pipeline 901, a groove is disposed on the holder 909, an anti-skid pad 910 is embedded in the groove, an air cavity 907 is disposed in the positioning rod 804, an air plug 908 fixedly connected to the anti-skid pad 910 is slidably disposed in the air cavity 907, and the air cavity 907 is connected to the cavity 905 through a third pipeline 906;
specifically, in this embodiment, when piston 902 moved towards the direction of keeping away from driving-disc 801 centre of a circle end in cylinder 9, piston 902 pushed the cavity 905 with gas through second pipeline 901 in, then inputed to in the air cavity 907 through third pipeline 906, the effect of gas in the air cavity 907 pushed slipmat 910 and test tube outer wall butt through air lock 908, further fixed a position the test tube, effectively avoided the test tube to rock.
A feeding mechanism is further arranged on one side of the centrifugal box body 1, the feeding mechanism comprises a plurality of groups of vacuum suckers 708 arranged above the centrifugal box body 1, the vacuum suckers 708 are connected with a negative pressure cavity 705 through a fourth pipeline 707, and the negative pressure cavity 705 is fixedly connected with a vacuum pump 6 arranged on a lifting plate 703 through a fifth pipeline 601;
wherein, the lifting plate 703 is connected with a lifting mechanism;
each group of the fourth pipelines 707 is positioned and fixed by a cross plate 704;
specifically, in this embodiment, firstly, a test tube to be centrifuged is placed below the vacuum chuck 708, the vacuum pump 6 is started, the vacuum pump 6 generates negative pressure at the vacuum chuck 708 through the fifth pipeline 601 and the third pipeline 906, so as to adsorb the test tube, then the lifting plate 703 is driven by the lifting mechanism to move towards the direction of the centrifuge box 1, the test tube penetrates through the positioning groove 803 and is placed in the positioning cylinder 802, the test tube does not need to be manually placed, the stability is higher, and bacteria are prevented from entering the centrifuge box 1;
the lifting mechanism comprises an L-shaped support plate 101 fixedly arranged on the side wall of the centrifugal box body 1, a lifting box body 7 is fixedly arranged on one side of the L-shaped support plate 101, a screw 706 is arranged in the lifting box body 7 in a rotating mode, a nut 702 fixedly connected with the lifting plate 703 is arranged on the screw 706 in a threaded mode, and the screw 706 is fixedly connected with a second motor 701 arranged on the lifting box body 7;
specifically, in this embodiment, the second motor 701 is started, the second motor 701 drives the screw 706 to rotate, and the lifting plate 703 is driven to lift in the process that the nut 702 moves on the screw 706, so as to adjust the height.
As a further scheme of the embodiment of the invention, a sealing plate 2 for sealing the positioning groove 803 is rotatably arranged on the centrifugal box body 1, a plurality of groups of through holes 201 are correspondingly arranged on the sealing plate 2, and when the centrifugal device is not used, the sealing plate 2 is rotated to ensure that the through holes 201 are not communicated with the positioning groove 803;
an infrared detector 501 is arranged at the bottom of the cross plate 704, and a detector 5 for receiving infrared signals is correspondingly arranged on the handle 401;
the lifting mechanism is also provided with a controller module, a transmission module and an operation module, wherein the controller module is used for receiving signals, analyzing and outputting commands, the transmission module is used for transmitting the signals and the commands, and the operation module is used for receiving the commands and controlling the operation of the lifting mechanism;
starting a vacuum pump 6, adsorbing and fixing the test tube through a vacuum chuck 708, transmitting a first signal by an infrared detector 501, measuring the distance between the test tube and the test tube after the first signal is received by a detector 5, when the distance is detected to be R1, transmitting the received first signal to a controller module by using a transmission module, executing a first command by using the controller module, and starting a second motor 701 to operate by using an operation module to receive the first command to drive the test tube to move towards the direction of a positioning groove 803;
the infrared detector 501 emits a second signal, the detector 5 receives the second signal and then measures the distance between the infrared detector and the detector, when the distance is detected to be R2, the transmission module is used for sending the received second signal to the controller module, the controller module is used for executing a second command, and the operation module receives the second command to stop the operation of the second motor 701 and the vacuum pump 6.
Example 2
Step one, starting a vacuum pump 6, adsorbing and fixing a test tube through a vacuum chuck 708, transmitting a first signal by an infrared detector 501, measuring the distance between the test tube and the test tube after the first signal is received by a detector 5, when the distance is detected to be R1, sending the received first signal to a controller module by using a transmission module, executing a first command by using the controller module, and starting a second motor 701 to run by using a running module to receive the first command to drive the test tube to move towards a positioning groove 803;
the infrared detector 501 emits a second signal, the detector 5 receives the second signal and then measures the distance between the infrared detector and the detector, when the distance is detected to be R2, the transmission module is used for sending the received second signal to the controller module, the controller module is used for executing a second command, and the operation module receives the second command to stop the operation of the second motor 701 and the vacuum pump 6;
step two; the handle 401 is rotated, the handle 401 drives the main gear 811 to rotate through the rotating rod 4, the main gear 811 drives the turntable 809 to rotate through the ring gear 808, the turntable 809 enables the pin shaft 807 to move from the center of a circle end to the direction close to the center end in the arc-shaped groove 810 in the rotating process, and the pin shaft 807 drives the clamping seat 909 to move towards the axis direction through the positioning rod 804, so that the clamping and fixing of the test tube are realized;
step three, starting the first motor 3, wherein the output end of the first motor 3 drives the driving disc 801 to rotate in the rotating process, and the driving disc 801 drives the test tube in the positioning cylinder 802 to carry out centrifugation in the rotating process;
and step four, after the centrifugation is finished, dividing the biological sample into supernatant and exosomes in the test tube, enabling the supernatant to flow into a recovery tube, enabling the exosomes to flow into an exosome storage tube for storage, and completing the separation and extraction.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "left", "right", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation and a specific orientation configuration and operation, and thus, should not be construed as limiting the present invention. Furthermore, "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate member, or they may be connected through two or more elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
While one embodiment of the present invention has been described in detail, the description is only a preferred embodiment of the present invention and should not be taken as limiting the scope of the invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.
Claims (10)
1. The exosome filtering and extracting device is characterized by comprising a centrifugal box body (1), a positioning disc (8) and a driving disc (801) which are arranged in the centrifugal box body (1), wherein a plurality of groups of positioning cylinders (802) for positioning test tubes are arranged between the positioning disc (8) and the driving disc (801), and a plurality of groups of positioning grooves (803) communicated with the positioning cylinders (802) are formed in the positioning disc (8);
the test tube positioning device comprises a positioning cylinder (802), a clamping mechanism and a positioning mechanism, wherein the clamping mechanism is distributed in the positioning cylinder (802) and used for clamping and fixing the test tube placed in the positioning cylinder (802);
the centrifugal box is characterized by further comprising a first motor (3) arranged at the bottom of the centrifugal box body (1), a driving cylinder (301) is fixedly installed below the driving disc (801), and the output end of the first motor (3) is fixedly connected with the driving cylinder (301).
2. An exosome filtering and extracting device according to claim 1, characterized in that the holding mechanism comprises a plurality of sets of holding seats (909) arranged in a circumferential array in the positioning cylinder (802), the holding seats (909) are fixedly connected with positioning rods (804) arranged in a sliding manner between the circumferential sides of the positioning cylinder (802), and the positioning rods (804) are connected with a pushing mechanism driving the positioning rods to approach or depart from the axis of the positioning cylinder (802).
3. The exosome filtering and extracting device according to claim 2, wherein the pushing mechanism comprises a plurality of groups of rotating discs (809) rotatably arranged at the bottom of a driving disc (801), a plurality of groups of arc-shaped grooves (810) are formed in the surface of each rotating disc (809) in a circumferential array, the arc-shaped grooves (810) extend from the proximal end close to the rotating disc (809) to the distal end, pin shafts (807) are movably embedded in the arc-shaped grooves (810), a plurality of groups of driving grooves (806) are formed in the driving disc (801), the pin shafts (807) penetrate through the driving grooves (806) and are fixedly connected with the positioning rods (804), and return springs (805) are arranged outside the positioning rods (804);
the bottom of the rotary table (809) is provided with a ring gear (808), each group of ring gears (808) is fixedly connected with a main gear (811) which is rotatably arranged at the bottom of the driving disc (801), the main gear (811) is fixedly connected with a rotating rod (4) which is rotatably arranged between the positioning disc (8) and the driving disc (801), and the tail end of the rotating rod (4) is fixedly provided with a handle (401).
4. The exosome filtering and extracting device according to claim 3, wherein the clamping mechanism further comprises an adsorption mechanism, the adsorption mechanism comprises cylinder bodies (9) which are arranged between a positioning disc (8) and a driving disc (801) in a circumferential array, pistons (902) are arranged in the cylinder bodies (9), the cylinder bodies (9) are separated by the pistons (902) to form a first chamber (911) and a second chamber (912), the pistons (902) are fixedly connected with telescopic springs (903) which are arranged in the cylinder bodies (9), the second chamber (912) is connected with a first pipeline (904), and adsorption holes which are connected with the first pipeline (904) are arranged at the bottom of the positioning cylinder (802).
5. An exosome filtering and extracting device according to claim 4, characterized in that a cavity (905) is arranged in the side wall of the positioning cylinder (802), the first chamber (911) is communicated with the cavity (905) through a second pipeline (901), the holding seat (909) is provided with a groove, an anti-skid pad (910) is embedded in the groove, an air cavity (907) is arranged in the positioning rod (804), an air plug (908) fixedly connected with the anti-skid pad (910) is arranged in the air cavity (907) in a sliding manner, and the air cavity (907) is connected with the cavity (905) through a third pipeline (906).
6. The exosome filtering and extracting device according to claim 1, characterized in that a feeding mechanism is further arranged on one side of the centrifugal box body (1), the feeding mechanism comprises a plurality of groups of vacuum suction cups (708) arranged above the centrifugal box body (1), the vacuum suction cups (708) are connected with a negative pressure cavity (705) through a fourth pipeline (707), and the negative pressure cavity (705) is fixedly connected with a vacuum pump (6) arranged on the lifting plate (703) through a fifth pipeline (601);
wherein the lifting plate (703) is connected with a lifting mechanism; each group of the fourth pipelines (707) is positioned and fixed by a cross plate (704).
7. An exosome filtering and extracting device according to claim 6, characterized in that the lifting mechanism comprises an L-shaped support plate (101) fixedly mounted on the side wall of the centrifugal box body (1), a lifting box body (7) is fixedly mounted on one side of the L-shaped support plate (101), a screw (706) is rotatably arranged in the lifting box body (7), a nut (702) fixedly connected with the lifting plate (703) is arranged on the screw (706) in a threaded manner, and the screw (706) is fixedly connected with a second motor (701) arranged on the lifting box body (7).
8. An exosome filtering and extracting device according to claim 7, characterized in that the bottom of the cross plate (704) is provided with an infrared detector (501), and the handle (401) is correspondingly provided with a detector (5) for receiving infrared signals.
9. An exosome filtering and extracting device according to claim 8, characterized by further comprising a controller module, a transmission module and an operation module, wherein the controller module is used for receiving signals, analyzing and outputting commands, the transmission module is used for transmitting signals and commands, and the operation module is used for receiving commands and controlling the operation of the lifting mechanism.
10. An extraction method of the exosome-filtering extraction device according to any one of claims 1-9, characterized by comprising the following steps:
the method comprises the steps that firstly, a vacuum pump (6) is started, a test tube is adsorbed and fixed through a vacuum sucker (708), an infrared detector (501) emits a first signal, a detector (5) measures the distance between the test tube and the infrared detector after receiving the first signal, when the distance is detected to be R (1), the received first signal is sent to a controller module through a transmission module, a first command is executed through the controller module, and a running module receives the first command to start a second motor (701) to run to drive the test tube to move towards a locating groove (803);
the infrared detector (501) emits a second signal, the detector (5) receives the second signal and then measures the distance between the infrared detector and the detector, when the distance is detected to be R (2), the received second signal is sent to the controller module by using the transmission module, a second command is executed by using the controller module, and the operation module receives the second command and stops the operation of the second motor (701) and the vacuum pump (6);
step two; the test tube clamp is characterized in that a handle (401) is rotated, the handle (401) drives a main gear (811) to rotate through a rotating rod (4), the main gear (811) drives a rotary disc (809) to rotate through a ring gear (808), the rotary disc (809) enables a pin shaft (807) to move from a circle center end to a direction close to the center end in an arc-shaped groove (810) in the rotating process, and the pin shaft (807) drives a clamping seat (909) to move towards the axis center direction through a positioning rod (804) to clamp and fix a test tube;
step three, starting a first motor (3), wherein an output end of the first motor (3) drives a driving disc (801) to rotate in the rotating process, and the driving disc (801) drives test tubes in a positioning cylinder (802) to centrifuge in the rotating process;
and step four, after the centrifugation is finished, dividing the biological sample into supernatant and exosomes in the test tube, enabling the supernatant to flow into a recovery tube, enabling the exosomes to flow into an exosome storage tube for storage, and completing the separation and extraction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210851071.7A CN115193595A (en) | 2022-07-20 | 2022-07-20 | Exosome filtering and extracting device and exosome filtering and extracting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210851071.7A CN115193595A (en) | 2022-07-20 | 2022-07-20 | Exosome filtering and extracting device and exosome filtering and extracting method |
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| CN115193595A true CN115193595A (en) | 2022-10-18 |
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| CN202210851071.7A Withdrawn CN115193595A (en) | 2022-07-20 | 2022-07-20 | Exosome filtering and extracting device and exosome filtering and extracting method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115672769A (en) * | 2022-11-14 | 2023-02-03 | 杭州中欣晶圆半导体股份有限公司 | Detection device for automatically positioning and adsorbing silicon wafer and use method |
| CN118028084A (en) * | 2024-04-10 | 2024-05-14 | 水木星辰生物制药(深圳)有限公司 | System and method for filtering and extracting exosomes |
-
2022
- 2022-07-20 CN CN202210851071.7A patent/CN115193595A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115672769A (en) * | 2022-11-14 | 2023-02-03 | 杭州中欣晶圆半导体股份有限公司 | Detection device for automatically positioning and adsorbing silicon wafer and use method |
| CN118028084A (en) * | 2024-04-10 | 2024-05-14 | 水木星辰生物制药(深圳)有限公司 | System and method for filtering and extracting exosomes |
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