CN216106929U - Deciduous tooth pulp stem cell exosome extraction element - Google Patents

Abstract

The utility model discloses a breast tooth pulp stem cell exosome extraction device, which comprises a base, wherein a reaction barrel is arranged on the top surface of the base, the top surface of a side plate of the reaction barrel is in threaded connection with a barrel cover, a handle is fixedly connected to the top surface of the barrel cover, a semi-through mounting groove is arranged in the center of the top surface of the base, a rotating motor is vertically mounted in the mounting groove, the output end of the rotating motor is in transmission connection with a rotating shaft, the rotating shaft penetrates through the bottom surface of the reaction barrel and extends into the reaction barrel, a limiting block is fixedly sleeved on the outer edge of the rotating shaft, a positioning block is vertically fixed on the outer edge of the rotating shaft, a rotating head is sleeved on the outer edge of the rotating shaft, a positioning groove matched with the rotating shaft and the positioning block in shape is penetrated through the top surface of the rotating head, a plurality of placing ports distributed in an annular shape at equal intervals are semi-penetrated through the top surface of the rotating head, a clamping mechanism is arranged in the placing ports, and the top end of the rotating shaft is in threaded connection with a fixing sleeve, the scheme improves the extraction efficiency of the exosome of the deciduous tooth pulp stem cells and saves a large amount of time.

Description

Deciduous tooth pulp stem cell exosome extraction element

Technical Field

The utility model relates to the field of stem cell extraction devices, in particular to a deciduous tooth pulp stem cell exosome extraction device.

Background

Exosomes refer to small membrane vesicles containing complex RNA and proteins, which are nowadays specifically disc-shaped vesicles ranging in diameter from forty to one hundred nanometers, and are secreted by all cultured cell types and naturally occur in body fluids, including blood, saliva, urine, cerebrospinal fluid and milk. The traditional method for exosome separation is manual separation through a small machine, the efficiency of exosome density sample and gradient material extraction and separation is slow, exosome separation is inconvenient due to the mixing mode of exosomes, and the exosome separation is troublesome in separation and extraction

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects of the prior art, the utility model provides the deciduous tooth pulp stem cell exosome extraction device, which improves the extraction efficiency of the deciduous tooth pulp stem cell exosome, saves a large amount of time, reduces the workload of workers in a laboratory and further accelerates the overall progress of the experiment.

In order to solve the technical problems, the utility model provides the following technical scheme: a deciduous tooth pulp stem cell exosome extraction device comprises a base, wherein a reaction barrel is installed on the top surface of the base, a barrel cover is in threaded connection with the top surface of a side plate of the reaction barrel, a top surface fixed connection handle of the barrel cover is arranged, a semi-through installation groove is arranged at the center of the top surface of the base, a rotating motor is vertically installed in the installation groove, the output end of the rotating motor is in transmission connection with a rotating shaft, the rotating shaft penetrates through the bottom surface of the reaction barrel and extends to the inside of the reaction barrel, a limiting block is fixedly sleeved at the outer edge of the rotating shaft and is positioned at the lower end of the inside of the reaction barrel, a positioning block is vertically fixed at the outer edge of the rotating shaft, a rotating head is sleeved at the outer edge of the rotating shaft, a positioning groove matched with the rotating shaft and the positioning block in shape is penetrated through the top surface of the rotating head, and a plurality of placing openings distributed in an annular shape at equal intervals are semi-communicated with the top surface of the rotating head, the inside of placing the mouth is equipped with fixture, the fixed cover of top threaded connection of pivot.

Preferably, fixture is fixed in including the level place intraoral telescopic link, the terminal fixed connection arc snap ring of telescopic link, the spring is cup jointed to the outer fringe of telescopic link, a plurality of rotating heads are cup jointed to the outer fringe of pivot, the bottom surface of rotating head is equipped with the recess.

Preferably, the positioning block is located right above the limiting block, and the distance between the positioning block and the top end piece of the rotating shaft is 30 mm.

Preferably, a second groove is formed in the center of the top surface of the inner wall of the barrel cover, a bearing is sleeved in the second groove, and the fixing sleeve is installed in an inner ring of the bearing.

Preferably, the front side of the base is fixedly connected with a controller, and the controller is electrically connected with the rotating motor.

Preferably, the bottom surface of the base is vertically fixed with supporting legs, and a damping spring is arranged between the supporting legs and the bottom surface of the base.

Compared with the prior art, the utility model can achieve the following beneficial effects:

1. the efficiency of deciduous tooth pulp stem cell exosome extraction has been improved through this scheme, has saved a large amount of time, has also alleviateed staff's in the laboratory work load simultaneously, has further accelerated the holistic progress of experiment.

2. Through setting up fixture to placing intraoral centrifuging tube and having played the fixed effect of centre gripping, avoid the centrifuging tube to break away from in placing the mouth at the rotatory in-process of rotating head to lead to the centrifuging tube breakage, make intraductal stoste leak out.

Drawings

FIG. 1 is an overall structural view of the present invention;

FIG. 2 is a main sectional view of the overall structure of the present invention;

FIG. 3 is a perspective view of the overall structure of the rotary head of the present invention;

FIG. 4 is an enlarged top view of the structure of FIG. 3 at A in accordance with the present invention;

wherein: 1. a base; 2. supporting legs; 3. a controller; 4. a reaction barrel; 5. a barrel cover; 6. a grip; 7. mounting grooves; 8. a rotating electric machine; 9. rotating the head; 10. positioning blocks; 11. fixing a sleeve; 12. a placement port; 13. a rotating shaft; 14. a groove; 15. a limiting block; 16. positioning a groove; 17. a telescopic rod; 18. a spring; 19. an arc-shaped snap ring.

Detailed Description

The present invention will be further described with reference to specific embodiments for the purpose of facilitating an understanding of technical means, characteristics of creation, objectives and functions realized by the present invention, but the following embodiments are only preferred embodiments of the present invention, and are not intended to be exhaustive. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative efforts belong to the protection scope of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified, and materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

Example (b):

as shown in figures 1-3, the utility model provides a deciduous tooth pulp stem cell exosome extraction device, which comprises a base 1, wherein a reaction barrel 4 is installed on the top surface of the base 1, the top surface of a side plate of the reaction barrel 4 is in threaded connection with a barrel cover 5, the top surface of the barrel cover 5 is fixedly connected with a handle 6, a semi-through installation groove 7 is arranged at the center position of the top surface of the base 1, a rotating motor 8 is vertically installed in the installation groove 7, the output end of the rotating motor 8 is in transmission connection with a rotating shaft 13, the rotating shaft 13 penetrates through the bottom surface of the reaction barrel 4 and extends into the reaction barrel 4, an outer edge fixing sleeve 11 of the rotating shaft 13 is connected with a limiting block 15, the limiting block 15 is positioned at the lower end inside the reaction barrel 4, a positioning block 10 is vertically fixed at the outer edge of the rotating shaft 13, the positioning block 10 is positioned right above the limiting block 15, the distance between the positioning block 10 and a top end piece of the rotating shaft 13 is 30mm, an outer edge rotating head 9 of the rotating shaft 13 is sleeved on the rotating head 9, a positioning groove 16 matched with the appearance of the rotating head 9 is penetrated through the rotating shaft 13 and the positioning block 10, the top surface of a rotating head 9 is semi-penetrated through a plurality of placing openings 12 which are distributed in an annular shape at equal intervals, a clamping mechanism is arranged inside the placing openings 12, the top end of a rotating shaft 13 is in threaded connection with a fixing sleeve 11, a second groove is formed in the center position of the top surface of the inner wall of a barrel cover 5, a bearing is sleeved in the second groove, the fixing sleeve 11 is installed in an inner ring of the bearing, a controller 3 is fixedly connected to the front side of a base 1, the controller 3 is electrically connected with a rotating motor 8, a supporting foot 2 is vertically fixed to the bottom surface of the base 1, and a damping spring is arranged between the supporting foot 2 and the bottom surface of the base 1;

when extracting the exosome of deciduous tooth pulp stem cells, firstly, a centrifuge tube filled with pulp stem cell stock solution is arranged in a placing opening 12 on a rotating head 9, after the centrifuge tube is fixed by the clamping mechanism, the barrel cover 5 on the reaction barrel 4 is opened, the positioning groove 16 on the top surface of the rotating head 9 is aligned with the rotating shaft 13, inserting the rotating head 9, screwing the fixing sleeve 11, covering the barrel cover 5, starting the rotating motor 8 through the controller 3, driving the rotating shaft 13 to rotate by the rotating motor 8, driving the rotating head 9 to rotate by the rotating shaft 13, separating the pulp stem cell stock solution on the rotating head 9 under the action of the centrifugal force generated by the rotation of the rotating head 9, the scheme improves the extraction efficiency of the exosome of the deciduous tooth pulp stem cells, saves a large amount of time, meanwhile, the workload of workers in the laboratory is reduced, and the overall progress of the experiment is further accelerated.

As shown in fig. 1-4, the embodiment further discloses that the clamping mechanism includes a telescopic rod 17 horizontally fixed in the placing opening 12, an end of the telescopic rod 17 is fixedly connected with an arc-shaped snap ring 19, an outer edge of the telescopic rod 17 is sleeved with a spring 18, an outer edge of the rotating shaft 13 is sleeved with a plurality of rotating heads 9, and a bottom surface of each rotating head 9 is provided with a groove 14; through setting up fixture to placing the centrifuging tube in the mouth 12 and having played the fixed effect of centre gripping, avoid the centrifuging tube to break away from in placing the mouth 12 at the rotatory in-process of rotating head 9 to lead to the centrifuging tube breakage, make intraductal stoste leak out.

In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

The foregoing shows and describes the general principles, essential features, and advantages of the utility model. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims (6)

1. The utility model provides a deciduous tooth pulp stem cell exosome extraction element, includes base (1), top surface installation retort (4) of base (1), curb plate top surface threaded connection bung (5) of retort (4), top surface fixed connection handle (6) of bung (5), its characterized in that: the base (1) top surface central point puts half through mounting groove (7), install rotating electrical machines (8) perpendicularly in mounting groove (7), pivot (13) is connected in the output transmission of rotating electrical machines (8), pivot (13) run through the bottom surface of retort (4), and extend to the inside of retort (4), the fixed cover of outer fringe (11) of pivot (13) connects stopper (15), stopper (15) are located the lower extreme of retort (4) inside, the outer fringe vertical fixation locating piece (10) of pivot (13), rotating head (9) is cup jointed to the outer fringe of pivot (13), the top surface of rotating head (9) run through have with pivot (13) with constant head tank (16) that locating piece (10) appearance agrees with mutually, the top surface of rotating head (9) half runs through a plurality of equidistant annular distributions place mouth (12), the inside of placing mouth (12) is equipped with fixture, the fixed cover (11) of top threaded connection of pivot (13).

2. The extraction device of deciduous tooth pulp stem cell exosomes according to claim 1, characterized in that: fixture is fixed in including the level place telescopic link (17) in mouthful (12), terminal fixed connection arc snap ring (19) of telescopic link (17), spring (18) are cup jointed to the outer fringe of telescopic link (17), a plurality of rotating heads (9) are cup jointed to the outer fringe of pivot (13), the bottom surface of rotating head (9) is equipped with recess (14).

3. The extraction device of deciduous tooth pulp stem cell exosomes according to claim 1, characterized in that: the positioning block (10) is located right above the limiting block (15), and the distance between the positioning block (10) and the top end piece of the rotating shaft (13) is 30 mm.

4. The extraction device of deciduous tooth pulp stem cell exosomes according to claim 1, characterized in that: a second groove is formed in the center of the top surface of the inner wall of the barrel cover (5), a bearing is sleeved in the second groove, and the fixing sleeve (11) is installed in an inner ring of the bearing.

5. The extraction device of deciduous tooth pulp stem cell exosomes according to claim 1, characterized in that: the front side of the base (1) is fixedly connected with a controller (3), and the controller (3) is electrically connected with the rotating motor (8).

6. The extraction device of deciduous tooth pulp stem cell exosomes according to claim 1, characterized in that: the supporting legs (2) are vertically fixed on the bottom surface of the base (1), and damping springs are arranged between the supporting legs (2) and the bottom surface of the base (1).

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