CN220846128U - PBMC separating tube - Google Patents
PBMC separating tube Download PDFInfo
- Publication number
- CN220846128U CN220846128U CN202322294301.7U CN202322294301U CN220846128U CN 220846128 U CN220846128 U CN 220846128U CN 202322294301 U CN202322294301 U CN 202322294301U CN 220846128 U CN220846128 U CN 220846128U
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- Prior art keywords
- tube
- centrifugal
- cover
- hole
- pbmc
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- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 title claims abstract description 18
- 238000000926 separation method Methods 0.000 claims abstract description 13
- 210000004027 cell Anatomy 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 description 14
- 239000012528 membrane Substances 0.000 description 9
- 238000005119 centrifugation Methods 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 210000004443 dendritic cell Anatomy 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
Landscapes
- Centrifugal Separators (AREA)
Abstract
The utility model belongs to the technical field of cell separation tools, and particularly relates to a PBMC separation tube, which comprises a centrifuge tube and a tube cover, wherein the tube cover is in threaded connection with the centrifuge tube, a threaded hole is formed in the tube cover, the threaded hole is positioned on the axis of the tube cover, a centrifugal tube column is in threaded connection with the tube cover, a separation device is arranged on the centrifugal tube column, a through hole is formed in the tube cover, and a plug is detachably connected at the through hole and used for solving the problem that the separation device of the conventional PBMC separator cannot adjust the horizontal height.
Description
Technical Field
The utility model belongs to the technical field of cell separation tools, and particularly relates to a PBMC separation tube.
Background
PBMCs are peripheral blood mononuclear cells, i.e. cells with a single nucleus in the peripheral blood, comprising lymphocytes, monocytes, dendritic cells and other small numbers of cells. By isolating, screening and culturing PBMC, a large number of immune cells can be obtained for use in the corresponding immunotherapy.
The main separation method is Ficoll-Hypaque (polysucrose-diatrizoic amine) density gradient centrifugation, and separation is carried out due to the fact that the specific gravity of each component in blood is different. The mononuclear cells are densely arranged in the interface of the plasma layer and the separating liquid and are in a white membrane shape, and the PBMC can be obtained by sucking out the white membrane layer. The suction process of the tunica albuginea layer has a key effect on the cell yield, and the tunica albuginea layer is positioned between the plasma layer and the separated liquid layer because the tunica albuginea layer is easily scattered among the layering layers, so that the situation that the tunica albuginea layer cannot be sucked cleanly and remains much or liquid on a plurality of other layers is obtained.
In order to solve the problems, the Chinese patent application number 201620989776.5 discloses a centrifugal PBMC separator, which comprises a centrifugal tube, a centrifugal tube column and a centrifugal tube cover, wherein the centrifugal tube column is fixedly arranged in the centrifugal tube, the centrifugal tube cover is in threaded connection with the top of the centrifugal tube, and a horizontal separation device is arranged on the centrifugal tube column. When in use, the centrifugal blood sample is quantitatively injected into the centrifugal tube, the centrifugal tube cap is covered tightly, and the centrifugal operation is carried out. After centrifugation, the centrifugal pipe cap is opened, the inner rod is rotated, the rotary blades are unfolded to form a circumferential plane, and the white membrane layer is separated from the upper liquid layer. Sucking the upper liquid on the circumferential plane, rotating the inner rod to retract the rotating blades and sucking the white membrane layer. The separating device can separate the layered solution after centrifugation, ensure that the white membrane layer is completely sucked out, ensure the purity of the sucked-out white membrane layer, avoid mixing excessive interface-incapable liquid, and influence the PBMC yield by excessive residues.
But the centrifugal pipe column of this separator is fixed to be set up in the centrifuging tube, and separator is fixed relatively in the position of centrifugal pipe column, can not adjust separator's horizontal height wantonly, needs quantitative interpolation sample, uses inconveniently.
Disclosure of utility model
In view of the above, the present utility model aims to provide a PBMC separation tube, which solves the problem that the separation device of the existing PBMC separator cannot adjust the horizontal height.
The utility model solves the technical problems by the following technical means:
The utility model provides a PBMC separating tube, includes centrifuging tube and tube cover, tube cover and centrifuging tube threaded connection, is equipped with the screw hole on the tube cover, and the screw hole is located the axle center line of tube cover, and tube cover threaded connection has the centrifugal column, is equipped with separator on the centrifugal column, is equipped with the through-hole on the tube cover, and the through-hole department can be dismantled and be connected with the end cap.
Based on the technical scheme, the utility model also improves the following steps:
Further, the centrifugal pipe column comprises a threaded sleeve, the threaded sleeve is in threaded connection with the pipe cover, and an inner rod is rotatably arranged in the threaded sleeve; the separating device comprises a fixed blade and a rotating blade, wherein the fixed blade is fixed on the circumferential wall of a threaded sleeve, the rotating blade is rotatably sleeved on an inner rod, a hollow groove matched with the rotating blade is formed in the threaded sleeve, the central angle of the fixed blade is twice that of the rotating blade, and the rotating blade is unfolded to form a completed disc with the fixed blade.
Further, the top of interior pole is equipped with rubber antiskid cover, and the operating personnel of being convenient for rotates interior pole.
Further, scale marks are arranged on the tube wall of the centrifuge tube, so that the volume of liquid can be conveniently watched.
The utility model has the beneficial effects that: after the centrifugation is completed, the inner rod is rotated to enable the rotating blades to be unfolded into a circumferential plane, and the white membrane layer is separated from the upper liquid layer. Sucking the upper liquid on the circumferential plane, rotating the inner rod to retract the rotating blades and sucking the white membrane layer. Through rotating centrifugal pipe column can make separator reciprocate, can be according to the measuring specific height-adjusting of sample, it is more convenient to use.
Drawings
The utility model can be further illustrated by means of non-limiting examples given in the accompanying drawings;
FIG. 1 is a schematic perspective view of a separator tube according to an embodiment of the present utility model;
FIG. 2 is a cross-sectional view of a separator tube in an embodiment of the utility model;
FIG. 3 is a state diagram of a rotary blade in an embodiment of the present utility model when deployed;
FIG. 4 is a state diagram of the embodiment of the present utility model with the rotary blade retracted;
wherein, 1-centrifuging tube, 2-tube cap, 31-screw sleeve, 32-inner rod, 33-antiskid cover, 41-fixed blade, 42-rotating blade, 5-through hole, 6-end cap.
Detailed Description
The utility model will be described in detail below with reference to the attached drawings and specific examples:
As shown in fig. 1 to 4
The utility model provides a PBMC separating tube, includes centrifuging tube 1 and tube cap 2, tube cap 2 with centrifuging tube 1 threaded connection, be equipped with the screw hole on the tube cap 2, the screw hole is located on the axis of tube cap 2, threaded connection has centrifuging tube 1 post on the tube cap 2, be equipped with separator on the centrifuging tube 1 post, through rotating centrifuging tube 1 post can make separator reciprocate to can be according to the measuring tool body height-adjusting of sample, it is more convenient to use, be equipped with through-hole 5 on the tube cap 2, through-hole 5 department can dismantle and be connected with end cap 6, through-hole 5 can absorb liquid after the centrifugation in centrifuging tube 1.
Specifically, the centrifuge tube 1 column comprises a threaded sleeve 31, the threaded sleeve 31 is in threaded connection with the tube cover 2, and an inner rod 32 is rotatably arranged in the threaded sleeve 31;
The separating device comprises a fixed blade 41 and a rotating blade 42, wherein the fixed blade 41 is fixed on the circumferential wall of the threaded sleeve 31, the rotating blade 42 is rotatably sleeved on the inner rod 32, the threaded sleeve 31 is provided with a hollow groove matched with the rotating blade 42, the central angle of the fixed blade 41 is twice that of the rotating blade 42, the rotating blade 42 can be unfolded by rotating the inner rod 32, and the rotating blade 42 and the fixed blade 41 form a finished disc, so that a white film layer can be separated from other liquid on an upper layer or a lower layer, and the rotating blade 42 can be stored at the bottom of the fixed blade 41 when the inner rod 32 is rotated in the direction.
Specifically, a rubber anti-slip cover 33 is arranged at the top of the inner rod 32, so that an operator can conveniently rotate the inner rod 32.
Specifically, scale marks are arranged on the tube wall of the centrifuge tube 1, so that the volume of liquid can be conveniently watched.
The application method of the utility model is as follows: when in use, the centrifugal blood sample is injected into the centrifugal tube 1, the cover of the centrifugal tube 1 is closed, and the centrifugal operation is performed. After centrifugation, the inner rod 32 is rotated to spread the rotating blades 42 into a circumferential plane, separating the white film layer from the upper liquid layer. The upper liquid on the circumferential plane is sucked away, the inner rod 32 is rotated, the rotary blades 42 are retracted, and the white membrane layer is sucked. Through rotating centrifuging tube 1 post can make separator reciprocate, can be according to the measuring specific height-adjusting of sample, it is more convenient to use.
The above embodiments are only for illustrating the technical solution of the present utility model and not for limiting the same, and although the present utility model has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the technical solution of the present utility model, which is intended to be covered by the scope of the claims of the present utility model. The technology, shape, and construction parts of the present utility model, which are not described in detail, are known in the art.
Claims (3)
1. The utility model provides a PBMC separating tube, includes centrifuging tube (1) and tube cap (2), its characterized in that:
the centrifugal tube is characterized in that the tube cover (2) is in threaded connection with the centrifugal tube (1), a threaded hole is formed in the tube cover (2), the threaded hole is located on the axis of the tube cover (2), a centrifugal tube (1) column is in threaded connection with the tube cover (2), a separating device is arranged on the centrifugal tube (1) column, a through hole (5) is formed in the tube cover (2), and a plug (6) is detachably connected to the through hole (5);
The centrifuge tube (1) column comprises a threaded sleeve (31), the threaded sleeve (31) is in threaded connection with the tube cover (2), and an inner rod (32) is rotatably arranged in the threaded sleeve (31);
The separating device comprises a fixed blade (41) and a rotating blade (42), wherein the fixed blade (41) is fixed on the circumferential wall of the threaded sleeve (31), the rotating blade (42) is rotatably sleeved on the inner rod (32), the threaded sleeve (31) is provided with a hollow groove matched with the rotating blade (42), the central angle of the fixed blade (41) is twice that of the rotating blade (42), and the rotating blade (42) is unfolded to form a finished disc with the fixed blade (41).
2. The PBMC separation tube of claim 1, wherein:
The top of the inner rod (32) is provided with a rubber anti-skid sleeve (33).
3. The PBMC separation tube of claim 2, wherein:
Scale marks are arranged on the tube wall of the centrifuge tube (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322294301.7U CN220846128U (en) | 2023-08-25 | 2023-08-25 | PBMC separating tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322294301.7U CN220846128U (en) | 2023-08-25 | 2023-08-25 | PBMC separating tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220846128U true CN220846128U (en) | 2024-04-26 |
Family
ID=90784808
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322294301.7U Active CN220846128U (en) | 2023-08-25 | 2023-08-25 | PBMC separating tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220846128U (en) |
-
2023
- 2023-08-25 CN CN202322294301.7U patent/CN220846128U/en active Active
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant |