CN115165975A - Environment-friendly animal bacteria detection device and detection method thereof - Google Patents
Environment-friendly animal bacteria detection device and detection method thereof Download PDFInfo
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- CN115165975A CN115165975A CN202210897135.7A CN202210897135A CN115165975A CN 115165975 A CN115165975 A CN 115165975A CN 202210897135 A CN202210897135 A CN 202210897135A CN 115165975 A CN115165975 A CN 115165975A
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- 238000001514 detection method Methods 0.000 title claims abstract description 151
- 241000894006 Bacteria Species 0.000 title claims abstract description 79
- 239000007788 liquid Substances 0.000 claims abstract description 120
- 230000001580 bacterial effect Effects 0.000 claims abstract description 28
- 239000002699 waste material Substances 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 claims description 11
- 239000002184 metal Substances 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 3
- 238000005192 partition Methods 0.000 claims description 3
- 230000000712 assembly Effects 0.000 claims 1
- 238000000429 assembly Methods 0.000 claims 1
- 238000005516 engineering process Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000005096 rolling process Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 230000014670 detection of bacterium Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
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- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
- G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention relates to the technical field of bacteria detection, and discloses an environment-friendly animal bacteria detection device and a detection method thereof, wherein the detection device comprises a bacteria detection module, a solution storage cavity communicated with the bacteria detection module, and an impedance analyzer electrically connected with the bacteria detection module; the solution storage cavity comprises a first solution storage cavity for supplying liquid to the bacteria detection module and a second solution storage cavity for recovering waste liquid; the first liquid storage cavity comprises a first liquid supply cavity which is communicated with the bacteria detection module and used for storing a bacteria solution, and a second liquid supply cavity which is communicated with the bacteria detection module and used for storing a high-conductivity solution; the detection method comprises the following steps: s1, standing the solution; s2, matching and adjusting the flow rate; s3, micro-flow detection; the invention can improve the stability of the flow rate of the liquid in the microfluidic channel and ensure the accurate detection of the bacterial solution.
Description
Technical Field
The invention relates to the technical field of bacteria detection, in particular to an environment-friendly animal bacteria detection device and a detection method thereof.
Background
The conventional detection method of bacteria mainly comprises the steps of culturing, specifically culturing a bacteria sample by using a culture dish, and then identifying by using an electronic instrument; the method has the advantages of long period, harsh culture conditions, difficulty in rapid detection, low safety, easiness in causing pollution to the environment and no contribution to environmental protection.
For the detection of animal bacteria, the detection of etiology is generally carried out, and samples such as animal blood, secretion and the like are selected and then bacteria are cultured or smeared under a microscope to find bacteria, so that a long detection time is required, and rapid detection cannot be carried out.
Therefore, various bacteria detection methods based on the micro-fluidic chip are developed in the prior art; the micro-fluidic chip technology is a technology for controlling micro-fluid in a micron-sized channel, controlling the fluid in the micro-channel through a micro-sampling technology and implementing bacterial detection; when the bacteria liquid passes through the detection section by negative pressure suction, different pulse signals are generated due to different sizes and surface properties of the bacteria, and the pulse signals are amplified, sorted and accumulated for recording, so that the pulse signals can be converted into related information such as the number and the types of the bacteria.
The prior art can not well control the fluid in the microfluidic channel, so that the stability of the flow rate in the microfluidic channel provided by the prior microfluidic control technology is poor, and detection errors are easily caused.
Disclosure of Invention
The technical problem solved by the invention is as follows: provided is a micro-fluidic device for detecting bacteria, which improves the stability of the flow rate of liquid in a micro-fluidic channel and ensures accurate detection of bacteria.
The technical scheme of the invention is as follows: an environment-friendly animal bacteria detection device comprises a bacteria detection module, a solution storage cavity communicated with the bacteria detection module, and an impedance analyzer electrically connected with the bacteria detection module;
the solution storage cavity comprises a first liquid storage cavity for supplying liquid to the bacteria detection module and a second liquid storage cavity for recovering waste liquid;
the first liquid storage cavity comprises a first liquid supply cavity which is communicated with the bacteria detection module and used for storing a bacteria solution, and a second liquid supply cavity which is communicated with the bacteria detection module and used for storing a high-conductivity solution;
the bacteria detection module comprises a flow rate regulator communicated with the first liquid supply cavity and the second liquid supply cavity, a support frame arranged on the flow rate regulator, and a multi-channel detector arranged on the support frame and communicated with the flow rate regulator and the second liquid storage cavity;
the flow rate regulator comprises a first main plate, first-order regulators which are uniformly arranged on the first main plate and communicated with a first liquid supply cavity and a second liquid supply cavity, and second-order regulators which are uniformly arranged on the first main plate and one ends of which are communicated with the first-order regulators;
the multi-channel detector comprises a second main board and a plurality of micro-flow detection channels which are uniformly arranged on the second main board and are respectively and correspondingly communicated with the other end of the second-order regulator;
the first-order regulator comprises a protection box arranged on the first main board, a first negative pressure device arranged in the protection box and communicated with the first liquid supply cavity and the detection channel, and a second negative pressure device arranged in the protection box and communicated with the second liquid supply cavity and the micro-flow detection channel;
the second-order regulator comprises a conveying belt, a clamping component and a power device, wherein the conveying belt is arranged on the first main board, one end of the conveying belt is simultaneously communicated with the first negative pressure device and the second negative pressure device, the clamping component is movably arranged on the conveying belt, and the power device is arranged on the first main board and used for driving the clamping component;
the conveying belt comprises a flat flexible conveying belt body and two wear-resistant metal sheets which are correspondingly paved on the surface of the flexible conveying belt body respectively;
three pipelines arranged side by side are arranged in the flexible conveying belt body; the central pipeline is used for conveying a bacterial solution, and pipelines on two sides of the central pipeline are used for conveying a high-conductivity solution;
the clamping assembly clamps the wear-resistant metal sheet and can slide on the wear-resistant metal sheet along the extending direction of the flexible conveying belt body.
Furthermore, the micro-flow detection channel comprises a mounting box arranged on the second main board, a detection section arranged in the middle of the mounting box, and a liquid inlet assembly and a liquid discharge assembly which are arranged in the mounting box and are respectively positioned at the end part of the detection section;
one end of the mounting box is provided with a liquid inlet clamping socket for communicating the liquid inlet assembly with the conveying belt; a liquid discharge clamping socket for communicating the liquid discharge assembly with the second liquid storage chamber is arranged at the other end of the mounting box;
the liquid inlet component comprises a liquid inlet micro-pipe and a pipeline supporting piece, wherein one end of the liquid inlet micro-pipe is communicated with the liquid inlet clamping socket, and the other end of the liquid inlet micro-pipe is communicated with the detection section;
the liquid inlet microtubes comprise two conductive solution microtubes which are communicated with pipelines on two sides of the flexible conveying belt body through liquid inlet clamping jacks, and two bacterial solution microtubes which are arranged between the conductive solution microtubes and communicated with a central pipeline in the flexible conveying belt body.
The two conductive solution micro-tubes can effectively conduct the conductive solution, and the bacterial solution micro-tube can effectively conduct the bacterial solution; the flow rate of the two solutions can be independently adjusted by the diversion of the two pipelines, so that the flow rate can be conveniently controlled.
Furthermore, the detection section comprises a detection groove, an anode detection port arranged at one end of the detection groove and communicated with the conductive solution micro-tube and the bacterial solution micro-tube, and a cathode detection port arranged at the other end of the detection groove and communicated with the liquid drainage assembly.
The setting through positive pole detection mouth, negative pole detection mouth can be convenient for the impedance analysis appearance and detect.
Furthermore, two shunting clapboards are arranged inside one end of the detection tank close to the anode detection port; the detection groove forms three channels through a shunt partition plate, and the three channels are respectively communicated with the conductive solution micro-tube and the bacterial solution micro-tube;
one end of the detection groove close to the cathode detection port is a laminar flow mixing channel communicated with the premixing buffer channel and the liquid discharge assembly.
The laminar flow mixing channel is arranged, so that the high-conductivity solution and the bacterial solution with the same flow speed can form laminar flow, namely a stable interface is formed on the contact surface of the high-conductivity solution and the bacterial solution, and the interference of the high-conductivity solution on the bacterial solution is avoided under the condition of ensuring stable conductivity; ensuring stable detection of the bacterial solution.
Furthermore, an anode probe electrically connected with the anode of the impedance analyzer is arranged on the anode detection port;
and a cathode probe electrically connected with the cathode of the impedance analyzer is arranged on the cathode detection port.
The anode probe and the cathode probe can be directly connected with an impedance analyzer to implement accurate electrical impedance detection.
Further, first negative pressure ware, second negative pressure ware are including all setting up the negative pressure chamber in the protection box, setting up piston assembly in the negative pressure chamber sets up feed liquor pump on the negative pressure chamber inlet to and the check valve of setting on the negative pressure chamber inlet.
The interference of the liquid inlet pump to the pressure of the negative pressure cavity is cut off through the one-way valve, the problem of pressure instability caused by the liquid inlet pump is avoided, the size of the negative pressure cavity is controlled through the piston assembly to adjust the liquid pressure, and the stability of the pressure of the bacteria solution and the high-conductivity solution is ensured.
Further, a liquid outlet of the negative pressure cavity is provided with a flow and velocity sensor.
The setting of flow velocity sensor can carry out accurate detection to bacterium solution, high conducting solution, is convenient for control the flow velocity of bacterium solution, high conducting solution respectively.
Further, the power device comprises two groups of linear conveying devices which are respectively arranged on two sides of the conveying belt;
the linear conveying device comprises a strip-shaped support parallel to the conveying belt, rolling shafts uniformly arranged on the strip-shaped support, a conveying chain arranged on the rolling shafts, and a servo motor used for driving the rolling shafts;
the clamping assembly comprises two clamping conveyer belts and clamping plates respectively connected with the conveying chains.
The conveying chain can drive the clamping plates to advance, so that the clamping plates can slide stably on the conveying belt, and the liquid in the conveying belt can be pushed to flow stably through the sliding of the clamping plates.
The detection method adopting the environment-friendly animal bacteria detection device comprises the following steps:
s1, standing the solution
Putting a bacterial solution to be detected into the first liquid supply cavity; putting a high-conductivity solution into the second liquid supply cavity;
s2, flow rate matching adjustment
The first negative pressure device of the first-order regulator extracts the bacterial solution and conveys the bacterial solution into the middle pipeline of the flexible conveying belt body;
the second negative pressure device extracts the high-conductivity solution and conveys the solution into the other two pipelines of the flexible conveying belt body;
the liquid supply pressures of the two solutions are independently adjusted by adjusting the first negative pressure device and the second negative pressure device; driving the clamping assembly to slide on the flexible conveying belt body through a power device, and matching the flow rates of the two solutions to enable the flow rates of the bacteria solution and the high-conductivity solution to be consistent;
s3, microfluidic detection
And (3) introducing the bacterial solution with the consistent flow speed and the high-conductivity solution into the microfluidic detection channel to carry out impedance detection on the bacterial solution.
The invention has the beneficial effects that: the invention provides an environment-friendly animal bacteria detection device, which is a micro-fluidic device for bacteria detection, improves the stability of the flow rate of liquid in a micro-fluidic channel and ensures accurate detection of bacteria; compared with the traditional way of propelling fluid by a pump, the device can ensure the high stability of the liquid pressure in the negative pressure cavity through the first-order regulator to form a stable constant pressure cavity; the flow rates of the bacteria solution and the high-conductivity solution can be finely adjusted through the arrangement of the first negative pressure device and the second negative pressure device, so that the flow rates of the two solutions are consistent; the two solutions in the conveying belt are stably conveyed on one hand, and the flow rates of the two solutions can be buffered and matched on the other hand by adopting a sliding linear propulsion mode through the second-order regulator; thereby make two kinds of solutions form stable laminar flow boundary at laminar flow mixing channel, avoid two kinds of solution highly mixings for realize the improvement to the bacterium and detect the precision.
Drawings
FIG. 1 is a schematic structural view of the whole of embodiment 1 of the present invention;
FIG. 2 is a schematic view of the structure of a bacteria detection module according to embodiment 1 of the present invention;
FIG. 3 is a schematic view showing the construction of a flow rate regulator according to embodiment 1 of the present invention;
FIG. 4 is a schematic structural diagram of a second-order regulator according to embodiment 1 of the present invention;
FIG. 5 is a schematic diagram of the external structure of a microfluidic detection channel according to example 1 of the present invention;
FIG. 6 is a schematic diagram of the internal structure of a microfluidic detection channel according to example 1 of the present invention;
fig. 7 is a schematic structural view of a first negative pressure device and a second negative pressure device in embodiment 1 of the present invention;
FIG. 8 is a schematic structural view of a power plant in accordance with embodiment 3 of the present invention;
wherein, 1-bacteria detection module, 2-solution storage cavity, 20-first liquid storage cavity, 21-second liquid storage cavity, 200-first liquid supply cavity, 201-second liquid supply cavity, 3-impedance analyzer, 4-flow rate regulator, 40-first main plate, 41-first-order regulator, 42-second-order regulator, 43-power device, 410-protection box, 411-first negative pressure device, 412-second negative pressure device, 413-negative pressure cavity, 414-liquid inlet pump, 415-one-way valve, 416-piston assembly, 420-conveyer belt, 421-clamping assembly, 422-flexible conveyer belt body, 423-wear-resistant metal sheet, 430-bar bracket, 431-roller, 432-conveying chain, 433-servo motor, 5-bracket, 6-multichannel detector, 60-second main plate, 61-microfluidic detection channel, 62-detection section, 63-624 assembly, 64-liquid discharge assembly, 610-mounting box, 611-clamping connection, 612-liquid discharge clamping connection, 620-detection slot, 620-plug connection, 627-microfluidic detection channel, cathode-testing port, 626-623-micro-pipe connection, cathode-micro-tube detection channel, cathode-micro-tube, cathode-micro tube separation probe, and laminar flow detection probe, 632-micro tube.
Detailed Description
Example 1
As shown in fig. 1, the environment-friendly animal bacteria detection device comprises a bacteria detection module 1, a solution storage chamber 2 communicated with the bacteria detection module 1, and an impedance analyzer 3 electrically connected with the bacteria detection module 1;
the solution storage cavity 2 comprises a first liquid storage cavity 20 for supplying liquid to the bacteria detection module 1 and a second liquid storage cavity 21 for recovering waste liquid;
the first liquid storage chamber 20 comprises a first liquid supply cavity 200 communicated with the bacteria detection module 1 and used for storing bacteria solution, and a second liquid supply cavity 201 communicated with the bacteria detection module 1 and used for storing high-conductivity solution;
as shown in fig. 2, the bacteria detection module 1 includes a flow rate regulator 4 communicated with a first liquid supply cavity 200 and a second liquid supply cavity 201, a support frame 5 arranged on the flow rate regulator 4, and a multi-channel detector 6 arranged on the support frame 5 and communicated with the flow rate regulator 4 and a second liquid storage cavity 21;
the flow rate regulator 4 comprises a first main plate 40, first-order regulators 41 uniformly arranged on the first main plate 40 and communicated with a first liquid supply cavity 200 and a second liquid supply cavity 201, and second-order regulators 42 uniformly arranged on the first main plate 40 and having one ends communicated with the first-order regulators 41;
as shown in fig. 2, the multi-channel detector 6 includes a second main board 60, a plurality of micro-flow detection channels 61 uniformly disposed on the second main board 60 and respectively and correspondingly communicated with the other end of the second-order regulator 42;
as shown in fig. 3, the first-order regulator 41 includes a protection case 410 disposed on the first main plate 40, a first negative pressure unit 411 disposed in the protection case 410 and communicating the first liquid supply chamber 200 with the detection channel 61, and a second negative pressure unit 412 disposed in the protection case 410 and communicating the second liquid supply chamber 201 with the microfluidic detection channel 61;
as shown in fig. 3 and 4, the second-order adjuster 42 includes a conveyor belt 420 disposed on the first main plate 40 and having one end communicating with the first negative pressure device 411 and the second negative pressure device 412, a clamping assembly 421 movably disposed on the conveyor belt 420, and a power device 43 mounted on the first main plate 40 for driving the clamping assembly 421;
the conveying belt 420 comprises a flat-shaped flexible conveying belt body 422 and two wear-resistant metal sheets 423 which are correspondingly paved on the surface of the flexible conveying belt body 422 respectively;
three pipelines arranged side by side are arranged in the flexible conveying belt body 422; the central pipeline is used for conveying a bacterial solution, and pipelines on two sides of the central pipeline are used for conveying a high-conductivity solution;
the clamping assembly 421 clamps the wear-resistant metal piece 423 and can slide on the wear-resistant metal piece 423 along the extending direction of the flexible conveyor belt body 422.
As shown in fig. 5, the microfluidic detection channel 61 includes a mounting box 610 disposed on the second main plate 60, a detection section 62 disposed in the middle of the mounting box 610, and a liquid inlet assembly 63 and a liquid outlet assembly 64 disposed in the mounting box 610 and respectively located at the ends of the detection section 62;
one end of the mounting box 610 is provided with a liquid inlet clamping socket 611 for communicating the liquid inlet assembly 63 with the conveying belt 420; the other end of the mounting box 610 is provided with a drainage clamping socket 612 for communicating the drainage component 64 with the second liquid storage chamber 21;
as shown in fig. 6, the liquid inlet assembly 63 includes a liquid inlet micro pipe 630 having one end communicated with the liquid inlet clamping socket 611 and the other end communicated with the detection section 62, and a pipeline support 631 for fixing the liquid inlet micro pipe 630;
the liquid inlet micro-tube 630 comprises two conducting solution micro-tubes 632 which are communicated with pipelines on two sides of the flexible conveying belt body 422 through a liquid inlet clamping socket 611, and a bacterial solution micro-tube 633 which is arranged between the two conducting solution micro-tubes 632 and is communicated with a central pipeline in the flexible conveying belt body 422.
The detection section 62 comprises a detection groove 620, an anode detection port 621 arranged at one end of the detection groove 620 and communicated with a conductive solution micro-tube 632 and a bacteria solution micro-tube 633, and a cathode detection port 622 arranged at the other end of the detection groove 620 and communicated with a liquid discharge assembly 64.
Two shunting baffles 623 are arranged at one end of the detection tank 620 close to the anode detection port 621; the detection tank 620 forms three channels through a shunt partition 623 to form a premixing buffer channel 624 which is respectively communicated with the conductive solution micro-tube 632 and the bacterial solution micro-tube 633;
the end of the detection groove 620 near the cathode detection port 622 is a laminar flow mixing channel 625 which communicates the premixing buffer channel 624 with the drain assembly 64.
An anode probe 626 electrically connected with the anode of the impedance analyzer 3 is arranged on the anode detection port 621;
the cathode detection port 622 is provided with a cathode probe 627 electrically connected with the cathode of the impedance analyzer 3.
As shown in fig. 7, the first negative pressure device 411 and the second negative pressure device 412 include a negative pressure cavity 413 both disposed in the protection box 410, a piston assembly 416 disposed in the negative pressure cavity 413, an inlet pump 414 disposed on an inlet of the negative pressure cavity 413, and a check valve 415 disposed on an inlet of the negative pressure cavity 413.
And a flow and flow velocity sensor is arranged at a liquid outlet of the negative pressure cavity 413.
The inner wall diameters of the conductive solution micro-tube 632 and the bacterial solution micro-tube 633 are both 0.5mm.
The power device 43 is a roller screw, and drives the holding assembly 421 through the roller screw.
The roller screw, the flow and velocity sensor, the check valve 415, the liquid inlet pump 414, the piston assembly 416 and the impedance analyzer 3 are all products of the prior art, and the specific product types can be selected by those skilled in the art according to the needs.
Example 2
The embodiment is a detection method using the environment-friendly animal bacteria detection device in embodiment 1, and the method includes the following steps:
s1, standing the solution
Putting a bacteria solution to be detected into the first liquid supply cavity 200; a high-conductivity solution is put into the second liquid supply chamber 201;
s2, flow rate matching adjustment
The first negative pressure device 411 of the first-order regulator 41 extracts the bacterial solution and conveys the bacterial solution into the middle pipeline of the flexible conveying belt body 422;
the second negative pressure device 412 extracts the high conductivity solution and conveys the solution into the other two pipelines of the flexible conveying belt body 422;
the liquid supply pressures of the two solutions are independently adjusted by adjusting the first negative pressure device 411 and the second negative pressure device 412; then, the power device 43 drives the clamping assembly 421 to slide on the flexible conveying belt body 422, and the flow rates of the two solutions are matched, so that the flow rates of the bacterial solution and the high-conductivity solution are consistent;
s3, microfluidic detection
And (3) introducing the bacteria solution and the high-conductivity solution with consistent flow speed into the micro-flow detection channel 61 to carry out impedance detection on the bacteria solution.
Example 3
The difference from example 1 is:
as shown in fig. 8, the power device 43 includes two sets of linear conveying devices respectively disposed at two sides of the conveying belt 420;
the linear conveying device comprises a bar-shaped bracket 430 parallel to the conveyer belt 420, rollers 431 uniformly arranged on the bar-shaped bracket 430, a conveying chain 432 arranged on the rollers 431, and a servo motor 433 for driving the rollers 431;
the clamping assembly 421 includes two clamping belts 420 and clamping plates connected to the conveying chains 432, respectively.
Claims (9)
1. The environment-friendly animal bacteria detection device is characterized by comprising a bacteria detection module (1), a solution storage cavity (2) communicated with the bacteria detection module (1), and an impedance analyzer (3) electrically connected with the bacteria detection module (1);
the solution storage cavity (2) comprises a first liquid storage cavity (20) for supplying liquid to the bacteria detection module (1) and a second liquid storage cavity (21) for recovering waste liquid;
the first liquid storage chamber (20) comprises a first liquid supply cavity (200) which is communicated with the bacteria detection module (1) and is used for storing bacteria solution, and a second liquid supply cavity (201) which is communicated with the bacteria detection module (1) and is used for storing high-conductivity solution;
the bacteria detection module (1) comprises a flow rate regulator (4) communicated with a first liquid supply cavity (200) and a second liquid supply cavity (201), a support frame (5) arranged on the flow rate regulator (4), and a multi-channel detector (6) arranged on the support frame (5) and communicated with the flow rate regulator (4) and a second liquid storage cavity (21);
the flow rate regulator (4) comprises a first main plate (40), first-order regulators (41) which are uniformly arranged on the first main plate (40) and communicated with a first liquid supply cavity (200) and a second liquid supply cavity (201), and second-order regulators (42) which are uniformly arranged on the first main plate (40) and one ends of which are communicated with the first-order regulators (41);
the multi-channel detector (6) comprises a second main board (60), and a plurality of micro-flow detection channels (61) which are uniformly arranged on the second main board (60) and are respectively and correspondingly communicated with the other end of the second-order regulator (42);
the first-order regulator (41) comprises a protection box (410) arranged on the first main plate (40), a first negative pressure device (411) arranged in the protection box (410) and communicated with the first liquid supply cavity (200) and the detection channel (61), and a second negative pressure device (412) arranged in the protection box (410) and communicated with the second liquid supply cavity (201) and the micro-flow detection channel (61);
the second-order regulator (42) comprises a conveying belt (420) which is arranged on the first main plate (40) and one end of which is simultaneously communicated with the first negative pressure device (411) and the second negative pressure device (412), a clamping component (421) which is movably arranged on the conveying belt (420), and a power device (43) which is arranged on the first main plate (40) and is used for driving the clamping component (421);
the conveying belt (420) comprises a flat-shaped flexible conveying belt body (422) and two wear-resistant metal sheets (423) which are correspondingly paved on the surface of the flexible conveying belt body (422);
three pipelines arranged side by side are arranged in the flexible conveying belt body (422); the central pipeline is used for conveying a bacterial solution, and pipelines on two sides of the central pipeline are used for conveying a high-conductivity solution;
the clamping assembly (421) clamps the wear-resistant metal sheet (423) and can slide on the wear-resistant metal sheet (423) along the extending direction of the flexible conveying belt body (422).
2. The environment-friendly animal bacteria detection device according to claim 1, wherein the microfluidic detection channel (61) comprises a mounting box (610) arranged on the second main board (60), a detection section (62) arranged in the middle of the mounting box (610), and an inlet component (63) and an outlet component (64) which are arranged in the mounting box (610) and respectively arranged at the ends of the detection section (62);
one end of the mounting box (610) is provided with a liquid inlet clamping socket (611) for communicating the liquid inlet assembly (63) with the conveying belt (420); a liquid drainage clamping socket (612) for communicating the liquid drainage component (64) with the second liquid storage cavity (21) is arranged at the other end of the mounting box (610);
the liquid inlet assembly (63) comprises a liquid inlet micro pipe (630) and a pipeline supporting piece (631), wherein one end of the liquid inlet micro pipe is communicated with the liquid inlet clamping socket (611), and the other end of the liquid inlet micro pipe is communicated with the detection section (62);
the liquid inlet micro-tube (630) comprises two conductive solution micro-tubes (632) which are communicated with pipelines on two sides of the flexible conveying belt body (422) through a liquid inlet clamping socket (611), and a bacterial solution micro-tube (633) which is arranged between the two conductive solution micro-tubes (632) and communicated with a central pipeline in the flexible conveying belt body (422).
3. The environment-friendly animal bacteria detection device according to claim 2, wherein the detection section (62) comprises a detection groove (620), an anode detection port (621) arranged at one end of the detection groove (620) and communicated with the conductive solution micro-tube (632) and the bacterial solution micro-tube (633), and a cathode detection port (622) arranged at the other end of the detection groove (620) and communicated with the drainage assembly (64).
4. The environment-friendly animal bacteria detection device as claimed in claim 2, wherein two shunt baffles (623) are arranged inside the detection tank (620) near one end of the anode detection port (621); the detection tank (620) forms three channels through a shunt partition plate (623) to form a premixing buffer channel (624) which is respectively communicated with the conductive solution micro-tube (632) and the bacterial solution micro-tube (633);
one end of the detection groove (620) close to the cathode detection port (622) is provided with a laminar flow mixing channel (625) which is communicated with the premixing buffer channel (624) and the liquid discharge assembly (64).
5. The environment-friendly animal bacteria detection device according to claim 2, wherein the anode detection port (621) is provided with an anode probe (626) electrically connected to the anode of the impedance analyzer (3);
and a cathode probe (627) electrically connected with the cathode of the impedance analyzer (3) is arranged on the cathode detection port (622).
6. The environment-friendly animal bacteria detection device according to claim 1, wherein the first negative pressure device (411) and the second negative pressure device (412) comprise negative pressure cavities (413) which are arranged in the protection box (410), piston assemblies (416) arranged in the negative pressure cavities (413), liquid inlet pumps (414) arranged on liquid inlets of the negative pressure cavities (413), and one-way valves (415) arranged on liquid inlets of the negative pressure cavities (413).
7. The environment-friendly animal bacteria detection device of claim 6, wherein a liquid outlet of the negative pressure cavity (413) is provided with a flow rate sensor.
8. The environment-friendly animal bacteria detecting device as recited in claim 1, wherein the power device (43) comprises two sets of linear conveying devices respectively disposed at both sides of the conveyor belt (420);
the linear conveying device comprises a strip-shaped support (430) parallel to the conveying belt (420), rollers (431) uniformly arranged on the strip-shaped support (430), a conveying chain (432) arranged on the rollers (431), and a servo motor (433) for driving the rollers (431);
the clamping assembly (421) comprises two clamping conveyor belts (420) and clamping plates which are respectively connected with the conveying chains (432).
9. The method for detecting the environment-friendly animal bacteria detection device according to any one of claims 1 to 8, characterized by comprising the following steps:
s1, standing the solution
Putting a bacteria solution to be detected into the first liquid supply cavity (200); placing a high-conductivity solution into the second liquid supply cavity (201);
s2, flow rate matching adjustment
A first negative pressure device (411) of the first-order regulator (41) extracts the bacteria solution and conveys the bacteria solution into a middle pipeline of the flexible conveying belt body (422);
the second negative pressure device (412) extracts the high-conductivity solution and conveys the solution into the other two pipelines of the flexible conveying belt body (422);
the liquid supply pressures of the two solutions are independently adjusted by adjusting the first negative pressure device (411) and the second negative pressure device (412); then, the power device (43) drives the clamping assembly (421) to slide on the flexible conveying belt body (422) to match the flow rates of the two solutions, so that the flow rates of the bacteria solution and the high-conductivity solution are consistent;
s3, microfluidic detection
And (3) introducing the bacteria solution and the high-conductivity solution with consistent flow speed into the micro-flow detection channel (61) to carry out impedance detection on the bacteria solution.
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