CN115266896B - Biological sample analysis system and method - Google Patents
Biological sample analysis system and method Download PDFInfo
- Publication number
- CN115266896B CN115266896B CN202211170145.7A CN202211170145A CN115266896B CN 115266896 B CN115266896 B CN 115266896B CN 202211170145 A CN202211170145 A CN 202211170145A CN 115266896 B CN115266896 B CN 115266896B
- Authority
- CN
- China
- Prior art keywords
- biological sample
- electrode
- ionization
- ionization tube
- tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000012472 biological sample Substances 0.000 title claims abstract description 62
- 238000004458 analytical method Methods 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 18
- 150000002500 ions Chemical class 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 14
- 238000000605 extraction Methods 0.000 claims description 13
- 238000001514 detection method Methods 0.000 claims description 9
- 239000011159 matrix material Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 238000004949 mass spectrometry Methods 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 206010005003 Bladder cancer Diseases 0.000 description 2
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 description 2
- 239000000090 biomarker Substances 0.000 description 2
- 238000013375 chromatographic separation Methods 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 201000005112 urinary bladder cancer Diseases 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002705 metabolomic analysis Methods 0.000 description 1
- 230000001431 metabolomic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- -1 polydimethylsiloxane Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012284 sample analysis method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/0027—Methods for using particle spectrometers
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/0027—Methods for using particle spectrometers
- H01J49/0031—Step by step routines describing the use of the apparatus
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0431—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for liquid samples
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Robotics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention provides a biological sample analysis system and a method, wherein the biological sample analysis system comprises a clamping device, an ionization device and an analyzer, the clamping device is used for clamping the ionization device, the ionization device comprises a conduit, an electrode and a power supply, and the power supply supplies power to the electrode; further comprising: the two ends of the ionization tube are opened and are arranged at the bearing position of the bearing part; the cover body is arranged at the upper end opening of the ionization tube and is provided with a space for accommodating a biological sample and allowing the guide tube to enter; the conveying unit is connected with the catheter and is used for sucking and sending the biological sample into and out of the catheter in time; the clamping device is used for driving the catheter to enable the catheter to suck the biological sample in the space, enabling the catheter to penetrate through the cover body and enter the ionization tube, and driving the ionization tube to enable ions emitted from the outlet at the lower end of the ionization tube to enter the inlet of the analyzer. The invention has the advantages of automation and the like.
Description
Technical Field
The present invention relates to biological sample analysis, and more particularly to biological sample analysis systems and methods.
Background
Mass Spectrometry (MS) is a powerful tool due to its ability to analyze complex samples, with the most prominent advantages being its broad applicability and excellent quantification performance of biomarkers in complex biological samples at low concentration levels. MS-based metabolomics has been widely used to gain new insights into human, plant and microbial biochemistry, drug and biomarker discovery, as well as nutritional research and food safety control. However, MS use typically requires sample cleanup and chromatographic separation, e.g., combined Gas Chromatography (GC) and Liquid Chromatography (LC), requires complex procedures, expensive and sophisticated equipment, and is time consuming to operate.
By using open atmospheric pressure ionization, only little or no sample extraction or purification is needed, chromatographic separation is not needed, and the original sample in an environmental state can be directly ionized, such as viable bacterial colonies, single cells and the like growing in a culture dish, so that the method has great application prospects in the fields of pathogenic bacteria identification, precise medicine and the like.
However, biological sample detection based on open-type atmospheric pressure ionization is very complex, a platform is built for researchers at present, the system is unstable, automatic processing cannot be achieved, efficiency is extremely low, the amount of biological samples is extremely large, and the purpose of large-scale analysis cannot be met.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a biological sample analysis system.
The purpose of the invention is realized by the following technical scheme:
a biological sample analysis system comprising a grasping device and an analyzer; the biological sample analysis system further comprises:
an ionization device comprising a conduit, an electrode, and a power supply that supplies power to the electrode;
the ionization tube and the bearing piece are provided with openings at two ends and are arranged at the bearing position of the bearing piece;
a cover body provided at an upper end opening of the ionization tube and having a space for accommodating a biological sample and allowing the introduction of the guide tube;
a delivery unit connected to the catheter for drawing the biological sample into and out of the catheter at intervals;
the clamping device is used for driving the catheter to enable the catheter to suck the biological sample in the space, enabling the catheter to penetrate through the cover body and enter the ionization tube, and driving the ionization tube to enable ions emitted from the outlet at the lower end of the ionization tube to enter the inlet of the analyzer.
In order to reduce the structural complexity, further, the space is formed in a groove in the surface of the cover.
To improve the calculation accuracy, further, the biological sample analysis system further includes:
a detection unit for detecting a current I in the electrode;
a calculation unit for obtaining the depth of the conduit into the liquid in the ionization tube according to the current
ρ is the resistivity of the electrode, h is the length of the electrode, A is the area of the electrode, and V is the voltage applied to the electrode.
In order to improve the analysis precision, the analyzer is a mass spectrometer, and the cover body is made of flexible materials.
In order to improve the analysis efficiency, further, the bearing piece is provided with a plurality of bearing positions distributed in a matrix manner, and the ionization tube is vertically arranged at the bearing positions.
The invention also aims to provide an analysis method of a biological sample, and the aim of the invention is realized by the following technical scheme:
a method of analyzing a biological sample, the method comprising the steps of:
(A1) The clamping device clamps the guide pipe, and the guide pipe enters the space of the cover body; the cover body is arranged at an opening at the upper end of the ionization tube, and two ends of the ionization tube are opened and are arranged at the bearing position of the bearing piece;
(A2) The conveying unit works, and the biological sample in the space enters the conduit;
(A3) The clamping device drives the conduit to enable the conduit to penetrate through the cover body and enter the ionization tube;
(A4) The conveying unit works, the biological sample in the guide pipe enters the ionization pipe, and therefore the extraction of the biological sample is completed;
(A5) The clamping device drives the guide pipe, the ionization tube is separated from the bearing position, and an opening at the lower end of the ionization tube corresponds to an inlet of the analyzer;
(A6) The power supply applies voltage to the electrodes, the object to be detected in the ionization tube is ionized, and ions are emitted from the lower end opening and enter the inlet.
In order to ensure the ionization effect, further, in step (A3), a depth H of the conduit inserted into the liquid inside the ionization tube is obtained;
if the depth reaches the threshold value, entering the step (A4);
and if the depth does not reach the threshold value, increasing the depth.
To improve the calculation accuracy, further, the depth
ρ is the resistivity of the electrode, h is the length of the electrode, A is the area of the electrode, V is the voltage applied to the electrode, and I is the current in the electrode.
In order to reduce the structural complexity, further, the space is formed in a groove in the surface of the cover.
In order to improve the analysis accuracy, further, the liquid in the ionization tube is an extraction liquid, and the analyzer is a mass spectrometer
Compared with the prior art, the invention has the beneficial effects that:
1. automation;
the device has the advantages that the clamping devices (such as mechanical arms, three-dimensional mechanical arms and the like), the bearing parts of the bearing positions (such as matrix distribution), the conveying units and the like are configured, so that the automation of biological sample suction, extraction in the ionization tube, ionization tube transfer and ionization is realized, and the analysis efficiency of a large batch of biological samples is improved;
2. the structure is simple;
the culture of the biological sample is carried out in the space (such as the surface groove) on the cover body, thereby reducing the structural complexity;
3. the analysis result is accurate;
by detecting the insertion depth H, the insertion depth meets the requirement, the biological sample is completely immersed in the liquid (such as extraction liquid) in the ionization tube, the extraction is complete, and the detection accuracy of the biological sample is further improved.
Drawings
The disclosure of the present invention will become more readily understood with reference to the accompanying drawings. As is readily understood by those skilled in the art: these drawings are only for illustrating the technical solutions of the present invention and are not intended to limit the scope of the present invention. In the figure:
fig. 1 is a schematic configuration diagram of a biological sample analysis system according to an embodiment of the present invention;
fig. 2 is a schematic flow chart of a biological sample analysis method according to an embodiment of the present invention.
Detailed Description
Fig. 1-2 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and reproduce the invention. Some conventional aspects have been simplified or omitted for the purpose of explaining the technical solution of the present invention. Those skilled in the art will appreciate that variations or substitutions from these embodiments will be within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Thus, the present invention is not limited to the following alternative embodiments, but is only limited by the claims and their equivalents.
Example 1:
fig. 1 schematically shows a structural view of a biological sample analysis system according to embodiment 1 of the present invention, which, as shown in fig. 1, includes:
the ionization device comprises a clamping device, an ionization device and an analyzer, wherein the clamping device is used for clamping the ionization device, such as a mechanical arm, a three-dimensional mechanical arm and the like, the ionization device comprises a guide pipe 210, an electrode and a power supply, and the power supply supplies power to the electrode;
the ionization tube 221 and the bearing piece 110, wherein two ends of the ionization tube 221 are opened and are arranged at the bearing position 120 of the bearing piece 110;
a cover 220, wherein the cover 220 is disposed at the upper end opening of the ionization tube 221 and has a space, such as a groove, a cavity, etc., for accommodating a biological sample and allowing the conduit 210 to enter;
a delivery unit connected to the conduit 210 for drawing the biological sample into and out of the conduit 210 in time;
the gripping device is used for driving the conduit 210, so that the conduit 210 sucks the biological sample in the space, and the conduit 210 passes through the cover 220 and enters the ionization tube 221, and driving the ionization tube 221, so that the ions emitted from the lower outlet of the ionization tube 221 enter the inlet of the analyzer 3.
In order to reduce the complexity of the structure and the difficulty of the operation, further, the space is formed in the groove 223 of the surface of the cover 220.
In order to ensure the completeness of the extraction of the biological sample, so as to ensure the analysis accuracy, further, the biological sample analysis system further comprises:
a detection unit for detecting a current I in the electrode;
a calculation unit for obtaining the depth of the conduit 210 entering the liquid in the ionization tube 221 according to the current
ρ is the resistivity of the electrode, h is the length of the electrode, A is the area of the electrode, and V is the voltage applied to the electrode.
In order to improve the analysis accuracy, the analyzer 3 is a mass spectrometer, and the cover 220 is made of a flexible material.
In order to process a batch of samples, further, the carrier 110 has a plurality of carrier sites 120 distributed in a matrix, and the ionization tubes 221 are vertically disposed at the carrier sites 120.
Fig. 2 schematically shows a flowchart of a method for analyzing a biological sample according to an embodiment of the present invention, and as shown in fig. 2, the method for analyzing a biological sample includes the following steps:
(A1) The clamping device clamps the conduit 210, and the conduit 210 enters the space of the cover 220; the cover 220 is disposed at the upper end opening of the ionization tube 221, and the ionization tube 221 has two open ends and is disposed at the carrying position 120 of the carrying member 110;
(A2) The delivery unit is operated, the biological sample in the space enters the conduit 210;
(A3) The gripping device drives the conduit 210, so that the conduit 210 passes through the cover 220 and enters the ionization tube 221;
(A4) The conveying unit works, the biological sample in the conduit 210 enters the ionization tube 221, so that the extraction of the biological sample is completed;
(A5) The clamping device drives the guide pipe 210, the ionization tube 221 is separated from the bearing position 120, and the lower end opening of the ionization tube 221 corresponds to the inlet of the analyzer 3;
(A6) The power supply applies voltage to the electrodes, the object to be measured in the ionization tube 221 is ionized, and ions are emitted from the lower end opening and enter the inlet.
In order to ensure the completeness of the extraction of the biological sample, so as to ensure the analysis accuracy, further, in step (A3), the depth H of the insertion of the conduit 210 into the liquid in the ionization tube 221 is obtained;
if the depth reaches the threshold value, entering the step (A4);
and if the depth does not reach the threshold value, increasing the depth.
To improve the current detection accuracy, further, the depth
ρ is the resistivity of the electrode, h is the length of the electrode, A is the area of the electrode, V is the voltage applied to the electrode, and I is the current in the electrode.
In order to reduce the difficulty of the operation, further, the space is formed in the groove 223 on the surface of the cover 220.
Example 2:
an example of application of the biological sample analysis system and method according to example 1 of the present invention to single cell analysis of bladder cancer.
In the present application example, as shown in fig. 1, the bearing member 110 has a matrix-shaped bearing position 120, and each ionization tube 221 is adapted to be vertically disposed at the bearing position 120;
the guide pipe 210 is a glass pipe, and the two metal electrode plates are arranged on the guide pipe 210 and are isolated from each other; the ionization tube 221 is a glass tube, two ends of the ionization tube are open, the cover 220 is disposed at an upper end opening (for illustration, one end opening of the ionization tube 221 is disposed at the position of the bearing position 120, when the ionization tube 221 is horizontally disposed, the ionization tube 221 is also referred to as an upper end opening, and it is not indicated that the ionization tube 221 is vertically disposed all the time), the ionization tube 221 is filled with a cell extract, and the cell extract cannot leak from the lower end opening even if the ionization tube 221 is vertically disposed because the inner diameter of the ionization tube 221 is smaller; the cover body 220 is made of PDMS (polydimethylsiloxane), the conduit 210 is allowed to pass through, and the surface of the cover body 220 is provided with a groove 223 for accommodating cells and culture solution;
the clamping device adopts a manipulator, so that three-dimensional movement is improved; the delivery unit comprises a pump and the detection unit detects the current I in the electrodes, which detection unit is prior art; the analyzer 3 is a mass spectrometer.
As shown in fig. 2, the method for analyzing a biological sample according to the embodiment of the present invention, that is, the method for operating the biological sample analysis system according to the embodiment of the present invention includes the following steps:
(A1) The clamping device clamps the conduit 210, the conduit 210 selects the ionization tube 221 and enters the groove 223 on the surface of the cover 220; the ionization tube 221 is vertically at the carrying position 120;
(A2) The delivery unit is operated, and the biological sample (i.e., single cell of bladder cancer) in the space is sucked into the catheter 210;
(A3) The gripping device drives the duct 210, so that the duct 210 vertically passes through the cover 220 and enters the ionization tube 221;
the power supply applies voltage to the electrodes, and the detection unit detects current I in the electrodes;
the calculation unit obtains the depth H of the conduit 210 into the liquid in the ionization tube 221 according to the current,
if the depth reaches the threshold value, entering the step (A4);
if the depth does not reach the threshold value, the depth is increased;
in this embodiment, the voltage V is 5 volts, the length h of the metal electrode is 20mm (extending in a direction parallel to the axial direction of the catheter 210), and the area A is 1mm 2 ρ is 40 Ω · mm, threshold 0.051mm, so that when current I is 0.003133 ampere, the insertion depth H reaches the threshold;
(A4) The conveying unit works, the biological sample in the conduit 210 enters the ionization tube 221, and the cells counted in the ionization tube 221 are extracted by the extraction liquid, so that the extraction of the biological sample is completed;
(A5) The guide pipe 210 is driven by the clamping device, the ionization tube 221 is separated from the bearing position 120 (the guide pipe 210 is driven because the ionization tube 221 is lighter, namely the ionization tube 221 is driven), the lower end opening of the ionization tube 221 corresponds to the inlet of the analyzer 3, the central axis of the ionization tube 221 and the central axis of the inlet of the analyzer 3 are collinear, and the distance between the lower end outlet of the ionization tube 221 and the inlet of the analyzer 3 is 1-3cm;
(A6) A power supply applies 2-5KV voltage to the electrodes, the object to be detected in the ionization tube 221 is ionized, and ions are emitted from the lower end opening and enter the inlet;
the analyzer 3 operates to obtain information of the biological sample.
Claims (7)
1. A biological sample analysis system comprising a grasping device and an analyzer; characterized in that the biological sample analysis system further comprises:
an ionization device comprising a conduit, an electrode, and a power supply that supplies power to the electrode;
the ionization tube is provided with openings at two ends and is arranged at a bearing position of the bearing piece;
a cover disposed at an upper end opening of the ionization tube and having a space for accommodating a biological sample and allowing the introduction of the guide tube;
a delivery unit connected to the catheter for drawing the biological sample into and out of the catheter in time division;
a detection unit for detecting a current I in the electrode;
a calculation unit for obtaining the depth of the conduit into the liquid in the ionization tube according to the current
ρ is the resistivity of the electrode, h is the length of the electrode, a is the area of the electrode, V is the voltage applied to the electrode;
the clamping device is used for driving the catheter to enable the catheter to suck the biological sample in the space, enabling the catheter to penetrate through the cover body and enter the ionization tube, and driving the ionization tube to enable ions emitted from the outlet at the lower end of the ionization tube to enter the inlet of the analyzer.
2. The biological sample analysis system of claim 1, wherein the space is formed within a recess in the cap surface.
3. The system for analyzing a biological specimen of claim 1, wherein the analyzer is a mass spectrometer and the cover is made of a flexible material.
4. The biological sample analysis system of claim 1, wherein the carrier has a plurality of carrier sites arranged in a matrix, the ionization tubes being vertically disposed at the carrier sites.
5. A method of analyzing a biological sample, the method comprising the steps of:
(A1) The clamping device clamps the guide pipe, the guide pipe enters the space of the cover body, and the electrode is arranged on the guide pipe; the cover body is arranged at an opening at the upper end of the ionization tube, and two ends of the ionization tube are opened and are arranged at the bearing position of the bearing piece;
(A2) The conveying unit works, and the biological sample in the space is sucked into the conduit;
(A3) The clamping device drives the conduit to enable the conduit to penetrate through the cover body and enter the ionization tube; obtaining the depth of the conduit inserted into the liquid in the ionization tube
P is the resistivity of the electrode, h is the length of the electrode, A is the area of the electrode, V is the voltage applied to the electrode, and I is the current in the electrode;
if the depth reaches the threshold value, entering the step (A4);
if the depth does not reach the threshold value, the depth is increased;
(A4) The conveying unit works, and the biological sample in the guide tube enters the ionization tube, so that the extraction of the biological sample is completed;
(A5) The clamping device drives the guide pipe to drive the ionization tube to be separated from the bearing position, and an opening at the lower end of the ionization tube corresponds to an inlet of the analyzer;
(A6) The power supply applies voltage to the electrodes, the object to be measured in the ionization tube is ionized, and ions are emitted from the lower end opening and enter the inlet.
6. The method of analyzing a biological sample according to claim 5, wherein the space is formed in a groove on the surface of the cap.
7. The method of analyzing a biological specimen according to claim 5, wherein the liquid in the ionization tube is an extraction liquid, and the analyzer is a mass spectrometer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211170145.7A CN115266896B (en) | 2022-09-26 | 2022-09-26 | Biological sample analysis system and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211170145.7A CN115266896B (en) | 2022-09-26 | 2022-09-26 | Biological sample analysis system and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115266896A CN115266896A (en) | 2022-11-01 |
| CN115266896B true CN115266896B (en) | 2023-01-13 |
Family
ID=83757019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211170145.7A Active CN115266896B (en) | 2022-09-26 | 2022-09-26 | Biological sample analysis system and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115266896B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202120862U (en) * | 2010-07-06 | 2012-01-18 | 东华理工大学 | Normal pressure chemical extraction ionization source |
| CN103048378A (en) * | 2012-12-20 | 2013-04-17 | 上海华质生物技术有限公司 | Mass spectrum sampling and ionization device for direct extraction and ionization of sample, and method of device |
| JP2016218070A (en) * | 2015-05-18 | 2016-12-22 | 努 升島 | Electric field capture, free separation and molecular detection method of 1-cell or ultra-micro molecule |
| CN112067534A (en) * | 2020-09-26 | 2020-12-11 | 宁波大学 | Single cell mass spectrometry system and method |
| CN213022966U (en) * | 2020-07-01 | 2021-04-20 | 中国热带农业科学院农产品加工研究所 | Automatic sample introduction system of inductively coupled plasma mass spectrometer |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10269550B2 (en) * | 2014-02-21 | 2019-04-23 | Purdue Research Foundation | Systems and methods for quantifying an analyte extracted from a sample |
| WO2015126595A1 (en) * | 2014-02-21 | 2015-08-27 | Purdue Research Foundation | Analyzing an extracted sample using an immiscible extraction solvent |
| CN109904056B (en) * | 2017-12-11 | 2020-05-08 | 中国科学院大连化学物理研究所 | Chemical ionization-vacuum ultraviolet single photon ionization composite ionization source device based on air discharge |
| CN114400175A (en) * | 2021-12-01 | 2022-04-26 | 宁波大学 | Mass spectrometry system and method |
-
2022
- 2022-09-26 CN CN202211170145.7A patent/CN115266896B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202120862U (en) * | 2010-07-06 | 2012-01-18 | 东华理工大学 | Normal pressure chemical extraction ionization source |
| CN103048378A (en) * | 2012-12-20 | 2013-04-17 | 上海华质生物技术有限公司 | Mass spectrum sampling and ionization device for direct extraction and ionization of sample, and method of device |
| JP2016218070A (en) * | 2015-05-18 | 2016-12-22 | 努 升島 | Electric field capture, free separation and molecular detection method of 1-cell or ultra-micro molecule |
| CN213022966U (en) * | 2020-07-01 | 2021-04-20 | 中国热带农业科学院农产品加工研究所 | Automatic sample introduction system of inductively coupled plasma mass spectrometer |
| CN112067534A (en) * | 2020-09-26 | 2020-12-11 | 宁波大学 | Single cell mass spectrometry system and method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115266896A (en) | 2022-11-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU755104B2 (en) | Sample handling system for a multi-channel capillary electrophoresis device | |
| CN110579616B (en) | Micro-droplet treatment device and application method thereof | |
| CN112175809A (en) | Integrated nucleic acid detection workstation | |
| JP5313218B2 (en) | Bacteria testing system | |
| CN214150548U (en) | Double-channel portable organic pollutant detector | |
| CN102109423B (en) | Electrostatic field-based air sampler and sampling method thereof | |
| CN111122689A (en) | Tracing method for microbial pollution source | |
| CN108048296B (en) | Microorganism real-time separation detection method and application of separation detection device in microorganism separation detection identification | |
| CN115266896B (en) | Biological sample analysis system and method | |
| CN116676183B (en) | Molecular diagnosis system | |
| CN112444583B (en) | Cell detection device and method based on paper-based discharge technology | |
| CN2541842Y (en) | Capillary electrophoretic electrochmical detection chip | |
| Shao et al. | Controllable fabrication of pico/femtoliter pipette sampling probes and visual sample volume determination | |
| CN1275037C (en) | Non-contact conductivity detector and detection method | |
| CN214097497U (en) | Sample processing and detecting system | |
| CN214060493U (en) | Integrated nucleic acid detection workstation | |
| CN114910546B (en) | Single cell mass spectrometry device and method based on extraction technology | |
| CN210151104U (en) | Micro-fluidic chip and system comprising same | |
| CN109655519B (en) | Sex identification method for hermaphrodite plants | |
| CN212459419U (en) | Device for rapidly evaluating air bacterial pollution state | |
| US20230167478A1 (en) | Identifying and classifying microorganisms | |
| CN111670485A (en) | Automatic sampler | |
| CN114518325A (en) | Sample processing and detecting system | |
| CN219551663U (en) | Multi-channel full-automatic quartz crystal microbalance testing instrument | |
| CN217425384U (en) | Multichannel water biotoxicity detection structure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: West side of 1st floor, 1st floor, Building A, No. 288 Jingu Middle Road (East), Yinzhou District, Ningbo City, Zhejiang Province, 315000 Patentee after: CHINA INNOVATION INSTRUMENT Co.,Ltd. Country or region after: China Address before: Room 304, D Building, Kexin Building, 655 Xueshi Road, Yinzhou District, Ningbo City, Zhejiang Province, 315000 Patentee before: CHINA INNOVATION INSTRUMENT Co.,Ltd. Country or region before: China |
|
| CP03 | Change of name, title or address |