CN115152793A - Purifying agent for ICU ward purification and preparation method thereof - Google Patents

Purifying agent for ICU ward purification and preparation method thereof Download PDF

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Publication number
CN115152793A
CN115152793A CN202210881270.2A CN202210881270A CN115152793A CN 115152793 A CN115152793 A CN 115152793A CN 202210881270 A CN202210881270 A CN 202210881270A CN 115152793 A CN115152793 A CN 115152793A
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parts
icu ward
agent
purifying agent
polysaccharide
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CN202210881270.2A
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Chinese (zh)
Inventor
闫永
闫泽楷
南光日
刘建超
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Duchuntang Biotechnology Research Institute Heze Co ltd
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Duchuntang Biotechnology Research Institute Heze Co ltd
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Priority to CN202210881270.2A priority Critical patent/CN115152793A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00Disinfectants; Antimicrobial compounds or mixtures thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00Fungicides
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention discloses a purifying agent for ICU ward purification and a preparation method thereof, wherein the formula of the purifying agent consists of hexammonosaccharide, biological protein, polysaccharide fermentation extract, brown algae polysaccharide, chitosan, glucan, glycerol, protective agent and purified water; the purifying agent for ICU ward purification and the preparation method thereof provided by the invention have the advantages that the preparation process is simple, the product property is stable, the environment surface can be disinfected by adopting a spraying mode or a wiping mode, the environment surfaces are not corroded, the purifying agent does not need to be diluted when in use, the sterilization effect is rapid, the influenza virus killing effect is good, and the purifying agent is an ideal disinfection product for hospital ICU ward environment disinfection.

Description

Purifying agent for ICU ward purification and preparation method thereof
Technical Field
The invention relates to the technical field of purificant, in particular to a purificant for purifying an ICU ward and a preparation method thereof.
Background
The disinfectant is a medicine for killing pathogenic microorganisms on a transmission medium, eliminates the pathogenic microorganisms outside a human body, cuts off the transmission path of infectious diseases, achieves the purpose of controlling the infectious diseases, and is also a very effective means for controlling epidemic situations by disinfecting the living environment.
Ether, ethanol and alcohol are inflammable, and the disinfectant is far away from open fire when in use, is forbidden to be used in a spraying mode, and is mainly recommended to be used for hands by adopting a wiping mode.
Although high-concentration hydrogen peroxide has a strong sterilization effect, the hydrogen peroxide has large skin irritation and is not suitable for household environment disinfection, but the low-concentration hydrogen peroxide has poor stability and weak sterilization efficiency, two hydrogen atoms and two oxygen atoms exist in hydrogen peroxide molecules, and can slowly release abnormally active nascent oxygen atoms in water.
The quaternary ammonium salt is a good cleaning agent and is widely used for disinfecting the surface of a non-critical environment in a medical health-care environment, and the quaternary ammonium salt has good surface activity, can be highly gathered around microbial cells, changes the permeability of the microbial cells, and enables the microbial cells to be infiltrated outwards to block the metabolism of the microbial cells so as to kill the bacteria.
However, according to literature data, it is known that low-concentration hydrogen peroxide cannot inactivate hydrophilic viruses without lipid envelopes (poliovirus) and quaternary ammonium salt cannot inactivate hydrophilic viruses without lipid envelopes (poliovirus), so that the invention provides a purifying agent for purifying an ICU ward, which can inactivate influenza viruses and can be used for efficiently sterilizing the ICU ward environment of a hospital.
Disclosure of Invention
The invention aims to provide a purifying agent for ICU ward purification and a preparation method thereof, which aim to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a purifying agent for purifying an ICU ward comprises the following components: hexa-polyurethane sugar, biological protein, polysaccharide fermentation extract, brown algae polysaccharide, chitosan, glucan, glycerol, a protective agent and purified water.
Preferably, the purifying agent comprises the following raw materials in parts by weight: 0.05-1 part of hexa-amino sugar, 0.5-1 part of biological protein, 0.05-0.1 part of polysaccharide fermentation extract, 1-2 parts of brown algae polysaccharide, 1-2 parts of chitosan, 1-2 parts of glucan, 4-5 parts of glycerol, 1.5-2 parts of protective agent and 95-98 parts of purified water.
Preferably, the purifying agent specifically comprises the following raw materials in parts by weight: 0.8 part of hexa-amino sugar, 0.8 part of biological protein, 0.08 part of polysaccharide fermentation extract, 1.5 parts of brown algae polysaccharide, 1.5 parts of chitosan, 1.5 parts of glucan, 4.5 parts of glycerol, 1.8 parts of protective agent and 96 parts of purified water.
A preparation method of a purifying agent for purifying an ICU ward comprises the following steps: 1) In a ten-thousand-level purification workshop, adding purified water into a blending tank, and adjusting the pH value to be between 5 and 7; 2) Sequentially adding hexa-glucosamine, bioprotein, polysaccharide fermentation extract, brown algae polysaccharide, chitosan, dextran, and glycerol under stirring, and finally adding protective agent until completely dissolving; 3) After all the components are dissolved, the pH value is adjusted, and then the mixture is filled.
Preferably, each component in step 2) is dissolved and stirred uniformly, and then the next component is added.
Preferably, the pH value is adjusted to 3.5-7.0 in the step 3), the temperature is increased to 80-85 ℃, and the temperature is kept for 30 minutes.
Preferably, the aseptic product is prepared by heating to 121 ℃, preserving the heat for 30 minutes, then cooling to below 40 ℃, and canning.
Has the advantages that: the beneficial effects of the invention are: the purifying agent for ICU ward purification and the preparation method thereof provided by the invention have the advantages that the preparation process is simple, the product property is stable, the environment surface can be disinfected by adopting a spraying mode or a wiping mode, the purifying agent has no corrosion to various environment surfaces, the diluting is not needed when the purifying agent is used, the sterilization effect is quick, the effect of killing influenza viruses is good, and the purifying agent is an ideal disinfection product for hospital ICU ward environment disinfection.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
A purifying agent for purifying an ICU ward comprises the following components: hexa-glucosamine, biological protein, polysaccharide fermentation extract, brown algae polysaccharide, chitosan, glucan, glycerol, a protective agent and purified water.
In the invention, the purifying agent comprises the following raw materials in parts by weight: 0.05-1 part of hexa-amino sugar, 0.5-1 part of biological protein, 0.05-0.1 part of polysaccharide fermentation extract, 1-2 parts of brown algae polysaccharide, 1-2 parts of chitosan, 1-2 parts of glucan, 4-5 parts of glycerol, 1.5-2 parts of protective agent and 95-98 parts of purified water.
In the invention, the purifying agent specifically comprises the following raw materials in parts by weight: 0.8 part of hexa-amino sugar, 0.8 part of biological protein, 0.08 part of polysaccharide fermentation extract, 1.5 parts of brown algae polysaccharide, 1.5 parts of chitosan, 1.5 parts of glucan, 4.5 parts of glycerol, 1.8 parts of protective agent and 96 parts of purified water.
A preparation method of a purifying agent for purifying an ICU ward comprises the following steps: 1) In a ten-thousand-level purification workshop, adding purified water into a blending tank, and adjusting the pH value to be between 5 and 7; 2) Sequentially adding hexa-amino sugar, biological protein, polysaccharide fermentation extract, brown algae polysaccharide, chitosan, glucan and glycerol under stirring, and finally adding a protective agent until the materials are completely dissolved; 3) After all the components are dissolved, the pH value is adjusted, and then the mixture is filled.
In the invention, each component in the step 2) is dissolved and stirred uniformly, and then the next component is added.
In the invention, the PH value is adjusted to 3.5-7.0 in the step 3), the temperature is increased to 80-85 ℃, and the temperature is kept for 30 minutes.
In the invention, the aseptic requirement product is heated to 121 ℃, kept for 30 minutes, cooled to below 40 ℃ and canned.
Example 1
The purifying agent for the ICU ward purification consists of the following components: 0.05 part of hexa-amino sugar, 0.5 part of biological protein, 0.05 part of polysaccharide fermentation extract, 1 part of brown algae polysaccharide, 1 part of chitosan, 1 part of glucan, 4 parts of glycerol, 1.5 parts of protective agent and 95 parts of purified water.
A preparation method of a purifying agent for purifying an ICU ward comprises the following steps: 1) In a ten-thousand-level purification workshop, purified water is firstly added into a blending tank, and the pH value is adjusted to be between 5; 2) Sequentially adding hexa-glucosamine, bioprotein, polysaccharide fermentation extract, brown algae polysaccharide, chitosan, dextran, and glycerol under stirring, and finally adding protective agent until completely dissolving; 3) After all the components are dissolved, the pH value is adjusted, and then the mixture is filled.
In the invention, each component in the step 2) is dissolved and stirred uniformly, and then the next component is added.
In the invention, the pH value is adjusted to 3.5 in the step 3), the temperature is increased to 80 ℃, and the temperature is kept for 30 minutes.
In the invention, the aseptic requirement product is heated to 121 ℃, kept for 30 minutes, cooled to below 40 ℃ and canned.
Example 2
The purifying agent for the ICU ward purification consists of the following components: 1 part of hexa-glucosamine, 1 part of biological protein, 0.1 part of polysaccharide fermentation extract, 2 parts of brown algae polysaccharide, 2 parts of chitosan, 2 parts of glucan, 5 parts of glycerol, 2 parts of protective agent and 98 parts of purified water.
A preparation method of a purifying agent for purifying an ICU ward comprises the following steps: 1) In a ten-thousand-level purification workshop, purified water is firstly added into a blending tank, and the pH value is adjusted to be 7; 2) Sequentially adding hexa-glucosamine, bioprotein, polysaccharide fermentation extract, brown algae polysaccharide, chitosan, dextran, and glycerol under stirring, and finally adding protective agent until completely dissolving; 3) After all the components are dissolved, the pH value is adjusted, and then the mixture is filled.
In the invention, each component in the step 2) is dissolved and stirred uniformly, and then the next component is added.
In the invention, the pH value is adjusted to 7.0 in the step 3), the temperature is increased to 85 ℃, and the temperature is kept for 30 minutes.
In the invention, the aseptic requirement product is heated to 121 ℃, kept for 30 minutes, cooled to below 40 ℃ and canned.
Example 3
The purifying agent for the ICU ward purification consists of the following components: 0.8 part of hexa-glucosamine, 0.8 part of biological protein, 0.08 part of polysaccharide fermentation extract, 1.5 parts of brown algae polysaccharide, 1.5 parts of chitosan, 1.5 parts of glucan, 4.5 parts of glycerol, 1.8 parts of protective agent and 96 parts of purified water.
A preparation method of a purifying agent for purifying an ICU ward comprises the following steps: 1) In a ten-thousand-level purification workshop, purified water is firstly added into a blending tank, and the pH value is adjusted to 6; 2) Sequentially adding hexa-glucosamine, bioprotein, polysaccharide fermentation extract, brown algae polysaccharide, chitosan, dextran, and glycerol under stirring, and finally adding protective agent until completely dissolving; 3) After all the components are dissolved, the pH value is adjusted, and then the mixture is filled.
In the invention, each component in the step 2) is dissolved and stirred uniformly, and then the next component is added.
In the invention, the pH value of 5 in the step 3) is adjusted, the temperature is raised to 83 ℃, and the temperature is kept for 30 minutes.
In the invention, the aseptic product is required to be heated to 121 ℃, kept for 30 minutes, cooled to below 40 ℃ and canned. lgTCID 50 /ml
Purification agent verification experiment
1. Purpose of experiment
Observing the effect of the cleaning agent on the disinfection of environmental surfaces
2. Experimental Material
1) Self-made bacterium liquid
Adding 25g of the raw material of the Caesalpinia longata Jayan into 225ml of sterile normal saline, uniformly mixing, sucking 5ml of the mixture into a culture dish for culture, classifying grown colonies according to bacteria and fungi, and picking out the colonies into 2 bottles of 100ml of liquid culture medium for culture. Only bacterial colonies were picked out of one flask of medium, and bacterial + fungal colonies were picked out of the other flask of medium.
2) Purifying agent
The purifying agent is kept at 85 ℃ for 180min and then is placed at night.
3) Experimental device
Sterile culture dish, electrothermal blowing drying box, constant-temperature and constant-humidity culture box and electrothermal constant-temperature culture box
3. Experimental method
1) Diluting the prepared bacterial liquid containing bacteria to 10 -5 After mixing, 1ml of the bacterial liquid is sucked into a sterile culture dish, and 12ml of the bacterial liquid is sucked into 12 culture dishes. Diluting the prepared bacterial liquid containing bacteria and fungi to 10 -5 After mixing, 1ml of the bacterial liquid is sucked into a sterile culture dish, and 12ml is sucked into 12 culture dishes. Sterile saline 1ml was pipetted into the petri dish and 2ml total pipetted into 2 dishes for blank control. The concrete conditions are as follows
Figure BDA0003761800080000061
Remarking: the culture dish was placed in a sterile constant temperature and humidity incubator at 25 ℃ and sterilized in advance, and air-dried to perform the next experiment (air-dried for about 3 hours)
2) 1ml of each of the purifiers was pipetted into a sterile petri dish for culture, and each group was made in triplicate.
3) 3 pieces of the air-dried culture dishes with bacteria and 3 pieces of the culture dishes with bacteria and fungi are poured into a culture medium for culture, and the culture medium is used as a control. And wiping 3 dried culture dishes with bacteria and 3 culture dishes with bacteria and fungi by using an aseptic cotton stick, sampling, dissolving in 100ml of aseptic normal saline, soaking for half an hour, uniformly mixing the solution, and sucking 5ml of the solution into the culture dishes to prepare two parallel samples.
4) The remaining 6 dishes of bacteria and 6 dishes of bacteria + fungi were poured with about 15ml of the decontaminant. Note: placing the culture dish into a sterilized 28 ℃ sterile constant temperature and humidity incubator for air drying, and performing the next experiment (after air drying for 18 hours, performing the next step)
5) And (5) pouring 3 culture dishes which are dried in the step (4) and added with bacteria and 3 culture dishes of bacteria and fungi into a culture medium for culture. And 3, wiping and sampling 3 culture dishes with the bacteria and the fungi, which are dried in the step 3, by using a sterile cotton stick, dissolving the culture dishes in 100ml of sterile physiological saline, soaking for half an hour, uniformly mixing the solution, and sucking 5ml of the solution into the culture dishes to prepare two parallel samples. Finally, a blank (adding sterile physiological saline) culture dish is poured into the culture medium for culture.
4. Results of the experiment
TABLE 1 bacteria
Figure BDA0003761800080000071
TABLE 2 bacteria + fungi
Figure BDA0003761800080000072
5. Analysis of results
As can be seen from the analysis of Table 1, the average number of positive control bacteria cultured by directly pouring the bacteria into the medium was 840 ten thousand per dish, and the number of bacteria cultured by adding the purifying agent was 0 per dish. The average number of the positive control bacteria cultured by bacteria wiping sampling is 400 ten thousand per dish, and the number of the bacteria added with the purifying agent is 0 per dish. From the above results, it is understood that the effect of the cleaning agent on the sterilization of the bacterial surface is 100%.
As can be seen from the analysis of Table 2, the average number of the positive control mixed bacteria cultured by directly pouring the bacteria and fungi into the culture medium is 600 ten thousand per dish, and the number of the mixed bacteria after adding the purifying agent is 20 ten thousand per dish. The average number of the positive control mixed bacteria for bacteria and fungi wiping sampling culture is 200 ten thousand per dish, and the number of the mixed bacteria after the purifying agent is added is 0 per dish. From the above results, it was found that the surface sterilization effect of the depurative was 97% for the culture dish cultured by directly pouring the culture medium, and 100% for the culture dish cultured by wiping the sample.
TABLE 1 attached with bacteria-carrying plate without purifying agent
Figure BDA0003761800080000081
TABLE 2 attached plate with purifying agent poured
Figure BDA0003761800080000082
Attached table 3 bacteriostatic performance table
Figure BDA0003761800080000091
TABLE 4 table of heavy metal content
Figure BDA0003761800080000092
The purifying agent for ICU ward purification and the preparation method thereof provided by the invention have the advantages that the preparation process is simple, the product property is stable, the environment surface can be disinfected by adopting a spraying mode or a wiping mode, the environment surfaces are not corroded, the purifying agent does not need to be diluted when in use, the sterilization effect is rapid, the influenza virus killing effect is good, and the purifying agent is an ideal disinfection product for hospital ICU ward environment disinfection.
The embodiments described above are preferred embodiments of the present invention, not all embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without inventive step based on the embodiments of the present invention, are within the scope of protection of the present invention.

Claims (7)

1. A purifying agent for purifying an ICU ward is characterized by comprising the following components: hexa-polyurethane sugar, biological protein, polysaccharide fermentation extract, brown algae polysaccharide, chitosan, glucan, glycerol, a protective agent and purified water.
2. The purifying agent for ICU ward purification according to claim 1, characterized by comprising the following raw materials by weight: 0.05-1 part of hexa-glucosamine, 0.5-1 part of bioprotein, 0.05-0.1 part of polysaccharide fermentation extract, 1-2 parts of brown algae polysaccharide, 1-2 parts of chitosan, 1-2 parts of glucan, 4-5 parts of glycerol, 1.5-2 parts of protective agent and 95-98 parts of purified water.
3. The purifying agent for ICU ward purification according to claim 2, characterized by comprising the following raw materials by weight: 0.8 part of hexa-amino sugar, 0.8 part of biological protein, 0.08 part of polysaccharide fermentation extract, 1.5 parts of brown algae polysaccharide, 1.5 parts of chitosan, 1.5 parts of glucan, 4.5 parts of glycerol, 1.8 parts of protective agent and 96 parts of purified water.
4. A preparation method of a purifying agent for purifying an ICU ward is characterized by comprising the following steps: 1) In a ten-thousand-level purification workshop, adding purified water into a blending tank, and adjusting the pH value to be between 5 and 7; 2) Sequentially adding hexa-amino sugar, biological protein, polysaccharide fermentation extract, brown algae polysaccharide, chitosan, glucan and glycerol under stirring, and finally adding a protective agent until the materials are completely dissolved; 3) After all the components are dissolved, the pH value is adjusted, and then the mixture is filled.
5. The method for preparing a decontaminating agent for ICU ward purification according to claim 4, wherein: in the step 2), each component is dissolved and stirred uniformly, and then the next component is added.
6. The method for preparing a decontaminating agent for ICU ward purification according to claim 4, wherein: adjusting the pH value to 3.5-7.0 in the step 3), heating to 80-85 ℃, and keeping the temperature for 30 minutes.
7. The method of claim 4 for preparing a decontaminant for ICU ward decontamination, wherein the decontaminant comprises: the product is required to be heated to 121 ℃ under the aseptic requirement, is kept for 30 minutes, is cooled to below 40 ℃, and is canned.
CN202210881270.2A 2022-07-25 2022-07-25 Purifying agent for ICU ward purification and preparation method thereof Pending CN115152793A (en)

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