CN115152792A - Saline-alkali resistant reagent for improving forage oat and use method thereof - Google Patents

Saline-alkali resistant reagent for improving forage oat and use method thereof Download PDF

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CN115152792A
CN115152792A CN202210637063.2A CN202210637063A CN115152792A CN 115152792 A CN115152792 A CN 115152792A CN 202210637063 A CN202210637063 A CN 202210637063A CN 115152792 A CN115152792 A CN 115152792A
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saline
oat
alkali
forage
concentration
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万燕
曾庆晨
邹亮
齐安银
李颜秘
黄静玮
向达兵
邬晓勇
吴琪
叶雪玲
刘长英
赵钢
彭镰心
姜良珍
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Chengdu University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16Heavy metals; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00Plant growth regulators

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
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  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
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  • Forests & Forestry (AREA)
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  • Chemical & Material Sciences (AREA)
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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a saline-alkali resistant reagent for improving forage oat and a use method thereof. The concentration of the ionic titanium in the saline-alkali resisting reagent is 10-30mg/L, and the concentration of the humic acid in the saline-alkali resisting reagent is 300-600 times of the solution. The saline-alkali resistant reagent is applied by watering or spraying. The saline-alkali resistant reagent can effectively improve the growth state of the oat in a saline-alkali environment, improve the fresh weight and dry weight of the oat, promote the root system growth of the oat and improve the content of crude protein and crude fiber in the oat.

Description

Saline-alkali resistant reagent for improving forage oat and use method thereof
Technical Field
The invention relates to the technical field of oat saline-alkali resistance planting, in particular to a saline-alkali resistance reagent for improving forage oat and a use method thereof.
Background
The salinization of soil is an important factor for limiting the yield and quality of crops, and the conditions of poor seed germination, reduced biomass, reduced plant height and the like of the crops are easily caused by the salinization environment, so that the harvest yield and the nutritional quality of the crops are seriously influenced.
Oats are excellent crops for annual food and feed, and are widely popularized due to their excellent characteristics of strong adaptability, high growth rate, capability of forming a large amount of biomass in a short time, and the like. In recent years, with the change of market demand and planting system, a large amount of oat is used as hay and silage for livestock to eat, so that the domestic oat grass demand is continuously expanded. Oat forage grass is an important component of daily ration of livestock, particularly, livestock husbandry in northwest, north China and other areas is developed, and a large amount of forage grass oat is needed, however, environmental conditions in the areas are mostly mountainous areas or mountains, rainfall is low, irrigation demand is large, and soil salinization phenomenon is common. Therefore, the forage oat is inevitably affected by saline-alkali stress in the planting process, so that the yield is reduced, and the nutrition and the feeding quality are reduced.
The cultivation measure reported in the current research on improving the yield and quality of oats in a saline-alkali environment is to apply an exogenous reagent to the oats, wherein the applied substance is usually H 2 S,H 2 O 2 And salicylic acid and the like. However, these agents all exhibit various drawbacks during use: wherein H 2 S is a highly toxic and flammable gas, has a certain saline-alkali resistance, but is complex to operate and has certain harm to a human body; h 2 O 2 Weak acid is easy to react with alkaline fertilizer to lose efficacy in the using process, is difficult to store and is easy to decompose at higher temperature; the amount and period of application of salicylic acid are determined specifically according to the degree of salt stress and growth of oats, and the effectiveness thereof is further examined.
At present, agents which are added externally to improve the stress resistance of crops are mostly single compounds, and related experiments are lacked to demonstrate that different application methods can affect the crops. The single crop growth regulator has a single effect on the growth and development of crops, and simultaneously has low effectiveness due to no synergistic effect with other growth regulators, the problem can be effectively solved by compounding the plant growth regulators with different action mechanisms, and researches on the combination of various mineral nutrients and plant regulators in which modes is favorable for improving the yield and quality of the oat are few, so that the researches on the reagents with different concentrations and the application methods thereof have important significance on the yield and quality improvement of the oat in the saline-alkali environment. Exploring the application method of the exogenous reagent can enable the reagent to better play a role, and reducing the using amount so as to save the cost. At present, related researches on improving the saline-alkali resistance of crops by a cultivation method of adding a reagent from an external source at home and abroad are rarely reported, and effective addition combinations and matched application methods are particularly rare.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a saline-alkali resistant reagent for improving forage oat and a use method thereof, wherein the saline-alkali resistant reagent can effectively improve the growth state of oat in a saline-alkali environment, improve the fresh weight and dry weight of oat and promote the growth of oat root system.
In order to achieve the purpose, the technical scheme adopted by the invention for solving the technical problems is as follows:
a saline-alkali resistant reagent for improving forage oat comprises humic acid and ion titanium.
Furthermore, the concentration of the ionic titanium in the saline-alkali resisting reagent is 10-30mg/L.
Furthermore, the concentration of the ionic titanium in the saline-alkali resisting agent is 20mg/L.
Furthermore, the concentration of humic acid in the saline-alkali resisting agent is 300-600 times of the solution.
Furthermore, the concentration of humic acid in the saline-alkali resisting reagent is 500 times of the solution.
Further, it is applied by spraying or irrigating on leaf surface.
Furthermore, the spraying frequency is 5-7 times.
Further, the amount of each spray was 15mL/100 strains.
Further, the spraying frequency is 1 time per day, and the spraying time is 17-19 hours per day.
Further, the spraying period is the three-leaf one-heart period of the seedlings.
The beneficial effects produced by the invention are as follows:
the main component of the humic acid used in the application is biochemical fulvic acid which has high loading capacity and high physiological activity, contains macro and micro nutrient substances, can be better utilized by plants, has the function of promoting the growth of the plants, can influence the stomatal opening degree of the leaf surfaces of the crops, reduces transpiration, and has a promotion effect on the stress resistance of the crops; the titanium element is closely related to physiological processes such as photosynthesis, nitrogen nutrition, enzyme activity and the like in the plant life process, acts on crops in an ion state, improves the conversion rate from zymogen to active enzyme, improves the chlorophyll conversion rate in stems and leaves of the crops, enhances the photosynthesis, has a special hormone effect, is beneficial to gene activation in plant cells, enables growth hormone to be conveyed to a growth center, promotes the differentiation of the plant cells to induce callus, thereby improving the plant resistance, increasing the yield of the crops and improving the quality of the crops.
In the application, the ionic titanium and the humic acid are used in a matched manner, so that the obvious synergistic effect is achieved in the aspect of enhancing the salt and alkali resistance of the oat, the salt and alkali resistance of crop seedlings can be obviously improved, the obvious enhancement effect is achieved compared with a single preparation, the application effect is not influenced by time, the salt and alkali resistance effect is good, the biochemical fulvic acid belongs to a plant growth regulator, is the best component in soil humus, the application amount of a chemical fertilizer can be reduced, and the soil pollution is reduced; the ionic titanium can not pollute the soil.
Detailed Description
Example 1
The saline-alkali resistant reagent for improving the forage oat comprises humic acid and ionic titanium, wherein the concentration of the ionic titanium is 10mg/L, and the concentration of the humic acid is 300 times of the solution.
Example 2
The saline-alkali resisting reagent for raising the content of forage oat consists of humic acid and ionic titanium, wherein the concentration of the ionic titanium is 10mg/L, and the concentration of the humic acid is 400 times of the solution.
Example 3
The saline-alkali resisting reagent for raising the content of forage oat consists of humic acid and ionic titanium, wherein the concentration of the ionic titanium is 10mg/L, and the concentration of the humic acid is 500 times of that of the solution.
Example 4
The saline-alkali resistant reagent for improving the forage oat comprises humic acid and ionic titanium, wherein the concentration of the ionic titanium is 10mg/L, and the concentration of the humic acid is 600 times of the solution.
Example 5
The saline-alkali resistant reagent for improving the forage oat comprises humic acid and ionic titanium, wherein the concentration of the ionic titanium is 20mg/L, and the concentration of the humic acid is 300 times of the solution.
Example 6
The saline-alkali resisting reagent for raising the content of forage oat consists of humic acid and ionic titanium, wherein the concentration of the ionic titanium is 20mg/L, and the concentration of the humic acid is 400 times of the solution.
Example 7
The saline-alkali resistant reagent for improving the forage oat comprises humic acid and ionic titanium, wherein the concentration of the ionic titanium is 20mg/L, and the concentration of the humic acid is 500 times of the solution.
Example 8
The saline-alkali resistant reagent for improving the forage oat comprises humic acid and ionic titanium, wherein the concentration of the ionic titanium is 20mg/L, and the concentration of the humic acid is 600 times of the solution.
Example 9
The saline-alkali resistant reagent for improving the forage oat comprises humic acid and ionic titanium, wherein the concentration of the ionic titanium is 30mg/L, and the concentration of the humic acid is 300 times of the solution.
Example 10
The saline-alkali resistant reagent for improving the forage oat comprises humic acid and ionic titanium, wherein the concentration of the ionic titanium is 30mg/L, and the concentration of the humic acid is 400 times of the solution.
Example 11
The saline-alkali resistant reagent for improving the forage oat comprises humic acid and ionic titanium, wherein the concentration of the ionic titanium is 30mg/L, and the concentration of the humic acid is 500 times of the solution.
Example 12
The saline-alkali resistant reagent for improving the forage oat comprises humic acid and ionic titanium, wherein the concentration of the ionic titanium is 30mg/L, and the concentration of the humic acid is 600 times of the solution.
Comparative example 1
A saline-alkali resistant reagent for improving forage oat is prepared from ionic titanium with concentration of 20mg/L.
Comparative example 2
A saline-alkali resisting reagent for improving forage oat is prepared from humic acid with the concentration of 500 times of solution.
Comparative example 3
The saline-alkali resistant reagent for improving the forage oat comprises humic acid and ionic titanium, wherein the concentration of the ionic titanium is 5mg/L, and the concentration of the humic acid is 200 times of the solution.
Comparative example 4
The saline-alkali resistant reagent for improving the forage oat comprises humic acid and ionic titanium, wherein the concentration of the ionic titanium is 40mg/L, and the concentration of the humic acid is 800 times of the solution.
Test examples
1. The experimental oat material is prepared by simulating a saline-alkali environment, and the preparation method comprises the following steps: salt-sensitive variety oat 'Qing permanent 233' is used as a test material, oat seeds 'Qing permanent 233' are soaked in 0.3% potassium permanganate solution, and disinfection is carried out for 20 minutes. Accelerating germination for 24 hours in a dark environment, selecting germinated seeds with consistent exposure, culturing in a plastic basin (diameter is 22cm, height is 15 cm) filled with nutrient soil, watering, placing in an indoor culture room for growth, keeping the temperature of 26 +/-6℃/20 +/-4℃, the relative humidity is 60-80%, the illumination intensity is 500-720 mu mol/square meter/s, thinning when two true leaves grow out from a seedling, keeping about 100 well-grown seedlings in each basin, irrigating roots by using mixed saline-alkali solution (prepared by Hoagland solution) with the concentration of 40mmol/L when 3 true leaves grow out from the seedling, and simulating a saline-alkali stress environment (the saline-alkali solution is NaCl: na ratio) 2 SO 4 ∶NaHCO 3 ∶Na 2 CO 3 = 12: 8: 9: 1). The ratio of 8:00 the water is poured with the water quantity of 1.5 times (about 1500 mL) of the water holding quantity of the nutrient soil, and the treatment concentration is kept constant in order to wash away the excessive salt. To avoid salt shock reactions, the saline-alkali solution is increased to 40mmol/L with a concentration difference of 10mmol/L per day.
2. Starting 10 days after the saline-alkali stress treatment, half of the tested oat material was taken, and the saline-alkali resistant reagents of examples 1 to 12 and comparative examples 1 to 4 and water (as a control group) were sprayed to the leaves of 3-leaf stage seedlings in each pot, 10 sets of each saline-alkali resistant reagent were repeated, 18 sprays per day at 1 time for 00 times, and 6 sprays were continuously applied at an amount of about 15mL per pot. The other half of the test oat material was taken and the 3-leaf stage seedling leaves in each pot were irrigated with the saline-alkali resistant agents of examples 1-12 and comparative examples 1-4 (as a control group), 10 sets of each saline-alkali resistant agent were repeated, 18 times per day at 18 00 irrigates for 6 successive irrigates, and the volume of irrigation was about 15mL per pot. After 5 days of growth, the fresh seedling weight, the dry root weight and the root length of 10 groups of oat seedlings in the spraying and irrigating mode are measured, the average value of 10 groups is taken as final data, and the specific result is shown in tables 1-2; the stem-leaf ratio and crude protein content of oat were determined, and the specific results are shown in tables 3-4.
Table 1: data for spraying anti-saline-alkali reagent
Figure BDA0003682509270000061
Figure BDA0003682509270000071
The data in the table show that after the saline-alkali resistant reagent in the embodiment 7 is sprayed, the oat has a good growth state in a saline-alkali stress environment, the fresh weight of the oat is increased, and the dry weight of seedlings and roots is also improved; in the comparative example, the growth state of the oats was inferior to that of the oats in the example, but the growth state was slightly improved compared to the oats to which the saline alkali resistance agent was not sprayed in the control group.
Table 2: data for irrigating saline-alkali resistant reagent
Figure BDA0003682509270000072
Figure BDA0003682509270000081
The data in the table show that after the saline-alkali resistant reagent in the example 7 is irrigated, the oat has a good growth state in a saline-alkali stress environment, the fresh weight of the oat is increased, and the dry weight of seedlings and roots is also improved; in the comparative example, the growth state of the oats was inferior to that of the oats in the example, but the growth state was slightly improved compared to the oats to which the saline alkali resistance agent was not sprayed in the control group.
The data in the tables 1 and 2 are compared to find that spraying the saline-alkali resistant reagent is more beneficial to improving the growth condition of the oat compared with irrigating the saline-alkali resistant reagent.
Table 3: data for spraying anti-saline-alkali reagent
Figure BDA0003682509270000082
Figure BDA0003682509270000091
The data in the table show that after the saline-alkali resistant reagent is sprayed, the stem-leaf ratio, the crude protein content and the crude fiber content of the oats in the examples 1-12 are all higher than those of the oats in the comparative example and the control group, and the saline-alkali resistant reagent can effectively improve the growth state of the oats and reduce the influence of a saline-alkali environment on the oats.
Table 4: data for irrigating saline-alkali resistant reagent
Figure BDA0003682509270000092
Figure BDA0003682509270000101
It can be known from the data in the table above that after the saline-alkali resistant reagent is irrigated, the stem-leaf ratio, the crude protein content and the crude fiber content of the oats in the examples 1-12 are all higher than those of the oats in the comparative examples and the control group, and the saline-alkali resistant reagent in the application can effectively improve the growth state of the oats and reduce the influence of the saline-alkali environment on the oats.
The data in the tables 1 and 2 are compared to find that spraying the saline-alkali resistant reagent is more beneficial to improving the growth condition of the oat compared with irrigating the saline-alkali resistant reagent.
3. Simulating a saline-alkali environment to prepare the experimental oat material again, and then taking the saline-alkali resistant reagent in example 7 as an example, spraying the saline-alkali resistant reagent on the leaves of 3-leaf stage seedlings in each pot, wherein the ratio of the saline-alkali resistant reagent to the leaves of the 3-leaf stage seedlings in each pot is 18: and the spraying amount is about 15mL per basin every time when the Chinese herbal medicine is sprayed for 1 time when the Chinese herbal medicine is sprayed for 3 times, 6 times and 9 times respectively. Two groups of repeat spraying times are set according to different spraying times, random variables are adopted for grouping, the crude protein content and the crude fiber content in the oat are measured after 10 days of growth, and specific results are shown in a table 5.
Table 5: crude protein content
Spraying for 3 times Spraying for 6 times Spraying for 9 times
Crude protein content (%) 5.73 6.5 5.93
Crude fiber content (%) 24.1 25.4 24.6
As can be seen from the data in the table, the continuous spraying of the salt and alkali resistant reagent for 6 times on the oats in the salt and alkali stress state can effectively improve the content of crude protein and crude fiber in the oats.

Claims (9)

1. A saline-alkali resistant reagent for improving forage oat is characterized in that the saline-alkali resistant reagent comprises humic acid and ionic titanium.
2. The forage oat salt and alkali resistance improving agent according to claim 1, wherein the concentration of ionic titanium in the salt and alkali resistance agent is 10-30mg/L.
3. The fed oat saline-alkali tolerant agent of claim 1 wherein the concentration of ionic titanium in the saline-alkali tolerant agent is 20mg/L.
4. The forage oat salt and alkali resistance improving agent according to claim 1, wherein the concentration of humic acid in the salt and alkali resistance agent is 300-600 times of the solution.
5. The forage oat salt and alkali resistance improving agent according to claim 1, wherein the concentration of humic acid in the salt and alkali resistance agent is 500 times of the solution.
6. The method of using the forage oat saline alkali resistance improving agent as claimed in any one of claims 1 to 5, wherein the agent is applied by foliar spraying or watering.
7. The use of the forage oat with increased saline and alkaline tolerance as defined in claim 6 wherein the spraying is performed 5 to 7 times.
8. The method of using the forage oat saline alkali resistance improving agent as claimed in claim 6 wherein the amount sprayed is 15mL/100 plants per time.
9. The method of using the forage oat saline alkali resistance increasing agent as claimed in claim 6 wherein the number of spraying is 1 per day.
CN202210637063.2A 2022-06-07 2022-06-07 Saline-alkali resistant reagent for improving forage oat and use method thereof Pending CN115152792A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109776164A (en) * 2017-11-13 2019-05-21 马仲起 A kind of high stability ion titanium being conducive to plant growth
CN109970497A (en) * 2019-04-04 2019-07-05 成都大学 A kind of salt stress-resistant agent and preparation method thereof for wheat crops
CN113248328A (en) * 2021-06-30 2021-08-13 黄垣耀 EM probiotic liquid titanium fertilizer and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109776164A (en) * 2017-11-13 2019-05-21 马仲起 A kind of high stability ion titanium being conducive to plant growth
CN109970497A (en) * 2019-04-04 2019-07-05 成都大学 A kind of salt stress-resistant agent and preparation method thereof for wheat crops
CN113248328A (en) * 2021-06-30 2021-08-13 黄垣耀 EM probiotic liquid titanium fertilizer and preparation method thereof

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