CN115152636B - Detoxification test tube seedling intensive culture device - Google Patents

Detoxification test tube seedling intensive culture device Download PDF

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Publication number
CN115152636B
CN115152636B CN202210972566.5A CN202210972566A CN115152636B CN 115152636 B CN115152636 B CN 115152636B CN 202210972566 A CN202210972566 A CN 202210972566A CN 115152636 B CN115152636 B CN 115152636B
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China
Prior art keywords
test tube
light guide
lamp
fluorescent lamp
culture
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CN202210972566.5A
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Chinese (zh)
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CN115152636A (en
Inventor
靖广义
靖永鹏
靖永宁
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Shandong Xiancao Biotechnology Co ltd
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Shandong Xiancao Biotechnology Co ltd
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Publication of CN115152636A publication Critical patent/CN115152636A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture

Abstract

The application provides a device for densely culturing detoxified test-tube plantlets, and mainly relates to the field of test-tube plantlet culture. The device comprises a culture rack, wherein a plurality of bearing tables are linearly arranged on the culture rack along the height direction; the bearing table is characterized in that a lamp cavity is arranged in the bearing table, the bottom surface of the lamp cavity is a grid plate, a plurality of slot holes are formed in the top surface of the lamp cavity along the length direction, a light guide plate is arranged in the slot, a plurality of fluorescent lamps are linearly arranged in the lamp cavity, the fluorescent lamps and the light guide plate are arranged under the light guide plate in one-to-one correspondence, lower reflecting plates are arranged on two sides of the bottom of the fluorescent lamp, an upper reflecting plate is arranged at the top of the fluorescent lamp, and the top of the upper reflecting plate is connected to two sides of the slot. The application has the beneficial effects that: the application can uniformly supplement light to the detoxified test tube plantlets, ensures the development of all test tube plantlets, ensures the balanced development of the densely arranged test tube plantlets, and can ensure the synchronous transplanting of the detoxified test tube plantlets.

Description

Detoxification test tube seedling intensive culture device
Technical Field
The application mainly relates to the field of test-tube seedling cultivation, in particular to a virus-free test-tube seedling intensive cultivation device.
Background
The quality of crops bred in multiple generations is gradually reduced, and the generation is inferior to the generation. The main reason for the decline of crop quality is the infection of plant viruses. In particular to the asexual propagation of crops, no safe and effective drug for treating the virus diseases accumulated in the asexual propagation process of the crops exists at present. In order to ensure the stability of the genetic material of the species, the plant nutrient body is adopted to carry out rapid propagation only by a plant tissue culture technology, so that the hereditary property of the original variety can be kept, and the uniformity of the variety germplasm is realized.
For the cultivation of the detoxified seedlings, usually, the cultivation is carried out in a greenhouse, and a fluorescent lamp is used for supplementing the light for the detoxified seedlings to simulate sunlight irradiation. However, in order to improve the culture efficiency, usually, the detoxified seedlings are densely arranged, and sometimes, the culture bottles of the detoxified seedlings are stacked, so that uneven light supplementing of the detoxified seedlings is caused, dysplasia of the detoxified seedlings is easily caused, and the detoxified seedlings cannot be transplanted in the same period due to unbalanced development.
Disclosure of Invention
In order to solve the defects in the prior art, the application provides a device for densely culturing detoxified test tube seedlings, which can uniformly supplement light to the detoxified test tube seedlings, ensure the development of all the test tube seedlings, ensure the uniform development of the densely arranged test tube seedlings and ensure the synchronous transplanting of the detoxified test tube seedlings.
The application aims to achieve the aim, and the aim is achieved by the following technical scheme:
the device comprises a culture rack, wherein a plurality of bearing tables are linearly arranged on the culture rack along the height direction, and the bearing tables and the culture rack are arranged in a sliding manner; the utility model discloses a fluorescent lamp, including the plummer, the plummer is equipped with the lamp chamber, the lamp chamber bottom surface is the grid plate, a plurality of slot holes form's slot is seted up along length direction to the lamp chamber top surface, set up the light guide plate in the slot, the lamp intracavity is the linearity and sets up a plurality of fluorescent lamps, fluorescent lamp and light guide plate one-to-one set up under the light guide plate, fluorescent lamp bottom both sides set up down the reflector, the reflector is narrow upper and lower wide toper down, and is adjacent the lower reflector bottom of fluorescent lamp is connected, the fluorescent lamp top sets up the reflector, the upper reflector is wide upper and lower narrow toper, upper reflector top is connected in the slot both sides.
The top of the light guide plate is provided with a cambered surface mirror corresponding to the fluorescent lamp, and the cambered surface mirror protrudes downwards.
The light guide plate is a light-transmitting plate with a cavity in the center.
The plurality of light guide plates in the same slot are arranged in a linear manner in the slot.
The fluorescent lamp is a cylindrical lamp tube, the fluorescent lamp is arranged along the length direction of the lamp cavity, and the lower reflecting plate and the upper reflecting plate are in butt joint with each other in the middle of the fluorescent lamp.
The upper reflecting plate is tightly contacted with the fluorescent lamp, the upper reflecting plate, the fluorescent lamp and the light guide plate are enclosed into an air channel, an exhaust fan and a temperature controller are arranged in the air channel, and the temperature controller controls the intermittent starting of the exhaust fan according to a temperature signal.
The top of the lamp cavity is made of a heat insulation plate.
The culture bottles on the bearing table are densely arranged in a single layer.
Four rows of culture bottles are arranged on the bearing table, and the fluorescent lamps are positioned at the bottoms between the first row and the second row of culture bottles and at the bottoms between the third row and the fourth row of culture bottles.
The height of the light guide plate is not more than two thirds of the height of the culture flask.
Compared with the prior art, the application has the beneficial effects that:
the application adopts the design that the lamp is arranged in the bearing table for light supplementing, and can supplement light above the densely arranged culture bottles. Meanwhile, the light source can also perform upper light supplementing through the light guide plate vertically arranged at the top of the bearing table, so that side light supplementing is performed for the densely arranged culture bottles, illumination received by the detoxified test tube seedlings in the culture bottles is more uniform, stable development of the detoxified test tube seedlings is ensured, development of the detoxified test tube seedlings is more uniform, and synchronous transplanting of test tube seedlings in the same batch is ensured.
This design adopts the mode of baffle light filling, can utilize same light source to realize that the top shines the operation of light filling and side light filling, has retrenched the structure of light filling, and the maneuverability is stronger. Meanwhile, the design of the light guide plate isolates the heat of a part of fluorescent lamps, and the influence of extra temperature on the development of test tube seedlings can be avoided.
Drawings
FIG. 1 is a schematic diagram of the front view of the present application;
FIG. 2 is a schematic view of the structure of the three-dimensional view angle of the application;
FIG. 3 is a schematic view of the cavity structure of the lamp of the present application;
FIG. 4 is a schematic view of the view angle structure under the carrying platform of the present application;
FIG. 5 is a schematic view of the structure of the carrying platform of the present application;
FIG. 6 is a schematic view of the installation structure of the light guide plate of the present application;
FIG. 7 is a schematic cross-sectional view of a carrier of the present application.
The reference numbers shown in the drawings: 1. a culture rack; 2. a carrying platform; 3. a luminaire cavity; 4. a grid plate; 5. a slot; 6. a light guide plate; 7. a fluorescent lamp; 8. a lower reflecting plate; 9. an upper reflector; 10. an air duct; 11. an exhaust fan; 12. culture flask.
Detailed Description
The application will be further described with reference to the accompanying drawings and specific embodiments. It is to be understood that these examples are illustrative of the present application and are not intended to limit the scope of the present application. Further, it will be understood that various changes and modifications may be made by those skilled in the art after reading the teachings of the application, and equivalents thereof fall within the scope of the application as defined by the claims.
As shown in figures 1-7, the intensive culture device for the detoxified test tube plantlets mainly updates an illumination system of the detoxified test tube plantlets, so that the detoxified test tube plantlets in the same batch are uniformly supplemented with light, and the development of the detoxified test tube plantlets is balanced. The device comprises a culture rack 1, wherein a plurality of bearing tables 2 are linearly arranged on the culture rack 1 along the height direction, and the bearing tables 2 are used as culture bottle placement positions of detoxified test tube seedlings. In this embodiment, only a single layer of culture flasks are arranged on the carrying table 2, and the height of the carrying table 2 is slightly larger than the height of the culture flasks.
In order to make more culture bottles bear in the same height space and save space cost, the height of the bearing table 2 is lower, so that the bearing table 2 and the culture rack 1 are slidably arranged for facilitating the taking and placing of the culture bottles. The telescopic slide rail is arranged between the culture rack 1 and the bearing table 2, so that the bearing table 2 can slide out of the culture rack 1, and the culture bottles can be placed and taken down conveniently.
In the application, fluorescent lamps are not installed on the side surface of the culture rack 1 for light supplementing, but the bearing table 2 is improved to a certain extent, and a lamp cavity 3 is arranged in the bearing table 2. And a fluorescent lamp 7 is arranged in the lamp cavity 3 for light supplementing.
The bottom surface of the lamp cavity 3 is provided with the grid plate 4, the grid plate 4 can transmit light well, and meanwhile, the lamp cavity 3 is protected and carried to a certain extent. The light rays emitted by the fluorescent lamp 7 arranged in the lamp cavity 3 can well penetrate through the grid plate 4 to supplement light to the top of the culture flask on the bearing table 2 below, and uniform illumination is provided for the growth and development of detoxified test tube seedlings in the culture flask. The top of the lamp cavity 3 is made of a heat-insulating plate, so that the heat of the fluorescent lamp 7 is prevented from affecting the development of detoxified test tube plantlets in the culture flask. The top surface of the lamp cavity 3 is provided with a plurality of slot-shaped slots 5 along the length direction, the light guide plate 6 is inserted and installed in the slots 5, the light guide plate 6 has good light path trafficability, and light can be conducted well, so that illumination is supplemented for detoxified test tube seedlings in culture flasks at two sides. Compared with a common one-side fluorescent lamp light supplementing method, the setting of the light guide plate 6 can take care of the inside detoxification test tube plantlets in the densely arranged culture bottles, and the problem that the detoxification test tube plantlets positioned at the inner side cannot obtain sufficient and uniform illumination because of the blocking of the culture bottles at the outer side is avoided, so that the detoxification test tube plantlets in the same batch can develop uniformly.
Specifically, the fluorescent lamp 7 adopts a cylindrical lamp tube, and the fluorescent lamp 7 is arranged along the length direction of the lamp cavity 3, so that the length direction of the bearing plate 2 can be covered, and sufficient and uniform illumination is provided for the detoxified test tube seedlings densely placed on the bearing plate 2. The fluorescent lamps 7 are arranged under the light guide plates 6 in one-to-one correspondence with the light guide plates 6. In order to make the light guide plate 6 be easily detached and installed, the light guide plate 6 in the same slot 5 has a plurality of light guide plates 6 which are linearly and tightly arranged in the slot 5. When the culture flask is inconvenient to take and put, the light guide plate 6 can be directly detached. The light guide plate 6 is a light-transmitting plate with a cavity in the center. More specifically, the light guide plate 6 is an acrylic plate.
In order to make fluorescent lamp 7 can carry out more even illumination to the detoxification test tube seedling of intensive range on the plummer 2 of below, reflector panel 8 is installed to fluorescent lamp 7 bottom both sides, reflector panel 8 is the toper of upper and lower width down, and is adjacent fluorescent lamp 7's lower reflector panel 8 bottom is connected to utilize a plurality of fluorescent lamps 7 to cover the detoxification test tube seedling of plummer 2 full range, and lower reflector panel 8 only covers the region that plummer 2 is located, can concentrate light and carry out the light filling to the detoxification test tube seedling, more energy saving.
The two sides of the top of the fluorescent lamp 7 are provided with the upper reflecting plate 9, the upper reflecting plate 9 is conical, the upper reflecting plate 9 is wide in upper part and narrow in lower part, and the top of the upper reflecting plate 9 is connected to the two sides of the slot 5. The upper reflector 9 can reflect and collect the light rays irradiated by the fluorescent lamp 7 upwards, so that the light rays are led into the light guide plate 6, and the light supplementing is carried out on the side surfaces of the detoxified test tube seedlings with the two sides by the light guide plate 6, so that the light supplementing of the detoxified test tube seedlings is more uniform.
The top of the light guide plate 6 is provided with a cambered surface mirror corresponding to the fluorescent lamp 7, and the cambered surface mirror protrudes downwards. The cambered surface mirror is a lens-free mirror with the bottom surface reflecting, so that the light reflected by the upper reflecting plate 9 can be reflected, and the light can be supplemented to the side surface of the detoxified test tube seedling through the light guide plate 6, so that the light is fully utilized.
Specifically, the lower reflector 8 and the upper reflector 9 are butted at the upper position of the middle part of the fluorescent lamp 7, so that the light of the fluorescent lamp 7 is divided into an upper layer and a lower layer, and the top light supplementing of the detoxified test-tube plantlet at the lower level is performed and the side light supplementing of the detoxified test-tube plantlet at the upper level is performed.
In this embodiment, four rows of culture flasks are arranged on the carrying table 2, and the fluorescent lamps 7 are positioned at the bottoms between the first row and the second row of culture flasks and at the bottoms between the third row and the fourth row of culture flasks. Thus, two fluorescent lamps 7 are arranged, and two groups of light guide plates 6 corresponding to the fluorescent lamps are arranged, so that side light supplementing is carried out on detoxified test tube seedlings in four rows of culture flasks. The height of the light guide plate 6 is not more than two thirds of the height of the culture flask, so that the placement and the taking out of the culture flask are affected as little as possible, and the checking and recording of the growth condition of the detoxified test tube plantlet are not affected too much. When placing the culture flask, the light guide plate 6 can also be used as a positioning device for the culture flask, so that the culture flask can be placed quickly and neatly.
More specifically, because the top of the fluorescent lamp 7 is closer to the top surface of the bearing table 2, heat generated by the fluorescent lamp 7 can act on the culture flask through the top surface of the bearing table 2, so that interference is caused to the detoxified test tube plantlet in the culture flask. Therefore, in the embodiment, the heat dissipation device can be increased to discharge the heat of the fluorescent lamp 7, and the adverse effect on the growth of the detoxified test tube plantlet is reduced. Specifically, the upper reflector 9 is closely contacted with the fluorescent lamp 7, and the upper reflector 9 can be contacted with the fluorescent lamp 7 through a flexible wire sealing ring. The upper reflecting plate 9, the fluorescent lamp 7 and the light guide plate 6 are enclosed to form an air duct 10, an exhaust fan 11 and a temperature controller are arranged in the air duct 10, and the temperature controller controls the exhaust fan 11 to be intermittently started according to a temperature signal. When the temperature in the air duct 10 is detected to rise to the upper limit due to the baking of the fluorescent lamp 7, the temperature controller controls the exhaust fan 11 to start accelerating air circulation, and the temperature of the light guide plate 6 and the top of the lamp cavity 3 is reduced.
When in use, the bearing table 2 is of a multi-layer structure on the culture rack 1, wherein no culture bottle is placed above the topmost bearing table 2, and no light guide plate 6 is arranged at the top of the topmost bearing table 2.
The device can gather most of the light of fluorescent lamp 7 through the lower reflector plate in the lamp cavity 3, carry out light filling for the blake bottle of putting on the plummer 2 of below, the upper reflector plate 9 at the top in the plummer 2 of below cooperates with light guide plate 6 simultaneously, can carry out light filling to the blake bottle side, make the detoxification test tube seedling in the blake bottle receive more even and sufficient illumination, guarantee the equilibrium that detoxification test tube seedling developed, make the detoxification test tube seedling growth condition of same batch unanimous, can guarantee transplanting simultaneously.

Claims (7)

1. The utility model provides a detoxification test tube seedling intensive culture device, includes cultivates frame (1), its characterized in that: a plurality of bearing tables (2) are linearly arranged on the culture rack (1) along the height direction, and the bearing tables (2) and the culture rack (1) are arranged in a sliding manner; the bearing table (2) is internally provided with a lamp cavity (3), the bottom surface of the lamp cavity (3) is provided with a grid plate (4), the top surface of the lamp cavity (3) is provided with a plurality of slot-shaped slots (5) along the length direction, the slots (5) are internally provided with light guide plates (6), the lamp cavity (3) is internally provided with a plurality of fluorescent lamps (7) in a linear manner, the fluorescent lamps (7) and the light guide plates (6) are arranged under the light guide plates (6) in a one-to-one correspondence manner, the tops of the light guide plates (6) are provided with cambered mirrors corresponding to the fluorescent lamps (7), the cambered mirrors are downwards protruded, the light guide plates (6) are light-transmitting plates with cavities in the center, and the light guide plates (6) in the same slot (5) are provided with a plurality of light guide plates (6) which are linearly arranged in the slots (5); the fluorescent lamp is characterized in that lower reflecting plates (8) are arranged on two sides of the bottom of the fluorescent lamp (7), the lower reflecting plates (8) are tapered with upper narrow sides and lower wide sides, the bottoms of the lower reflecting plates (8) of the fluorescent lamp (7) are adjacent to each other, an upper reflecting plate (9) is arranged at the top of the fluorescent lamp (7), the upper reflecting plate (9) is tapered with upper wide sides and lower narrow sides, and the tops of the upper reflecting plates (9) are connected to two sides of the slot (5).
2. The device for densely culturing the detoxified test tube plantlets according to claim 1, wherein the device is characterized in that: the fluorescent lamp (7) is a cylindrical lamp tube, the fluorescent lamp (7) is arranged along the length direction of the lamp cavity (3), and the lower reflecting plate (8) and the upper reflecting plate (9) are in butt joint with the middle part of the fluorescent lamp (7).
3. The device for densely culturing the detoxified test tube plantlets according to claim 1, wherein the device is characterized in that: the upper reflector (9) is tightly contacted with the fluorescent lamp (7), an air duct (10) is formed by enclosing the upper reflector (9), the fluorescent lamp (7) and the light guide plate (6), an exhaust fan (11) and a temperature controller are arranged in the air duct (10), and the temperature controller controls the exhaust fan (11) to be intermittently started according to a temperature signal.
4. The device for densely culturing the detoxified test tube plantlets according to claim 1, wherein the device is characterized in that: the top of the lamp cavity (3) is made of a heat insulation plate.
5. The device for densely culturing the detoxified test tube plantlets according to claim 1, wherein the device is characterized in that: the culture bottles on the bearing table (2) are densely arranged in a single layer.
6. The device for densely culturing detoxified test tube plantlets according to any one of claims 1-5, wherein: four rows of culture bottles are arranged on the bearing table (2), and the fluorescent lamps (7) are positioned at the bottoms between the first row and the second row of culture bottles and at the bottoms between the third row and the fourth row of culture bottles.
7. The device for densely culturing virus-free test tube plantlets according to claim 6, wherein: the height of the light guide plate (6) is not more than two thirds of the height of the culture flask.
CN202210972566.5A 2022-08-15 2022-08-15 Detoxification test tube seedling intensive culture device Active CN115152636B (en)

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CN202210972566.5A CN115152636B (en) 2022-08-15 2022-08-15 Detoxification test tube seedling intensive culture device

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Application Number Priority Date Filing Date Title
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CN115152636B true CN115152636B (en) 2023-09-29

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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5111612A (en) * 1989-04-25 1992-05-12 Shimizu Construction Co., Ltd. Plant culture apparatus
JP2005328718A (en) * 2004-05-18 2005-12-02 Harison Toshiba Lighting Corp Plant cultivation device
JP2011193832A (en) * 2010-03-23 2011-10-06 Stanley Electric Co Ltd Lighting device for raising plant and apparatus for raising plant using the same
CN202112129U (en) * 2011-05-17 2012-01-18 北方民族大学 Culture shelf for virus-free potato foundation seedlings
CN202374834U (en) * 2011-12-26 2012-08-15 哈尔滨商业大学 Plant tissue culture rack
CN202588022U (en) * 2012-06-01 2012-12-12 内蒙古凌志马铃薯科技股份有限公司 Cultivation frame
CN103155860A (en) * 2011-12-09 2013-06-19 江阴沃土农业生物科技开发有限公司 Potato tissue culture frame
CN204426096U (en) * 2014-12-03 2015-07-01 凌云县长生仙草生物科技开发有限公司 A kind of energy-conservation culturing rack with fluorescent lamp
CN205389852U (en) * 2016-03-23 2016-07-27 连云港市农业科学院 Frame is cultivateed in plant illumination
JP2017195838A (en) * 2016-04-28 2017-11-02 昭和電工株式会社 Plant cultivation-lighting apparatus for and plant cultivating apparatus using the same

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5111612A (en) * 1989-04-25 1992-05-12 Shimizu Construction Co., Ltd. Plant culture apparatus
JP2005328718A (en) * 2004-05-18 2005-12-02 Harison Toshiba Lighting Corp Plant cultivation device
JP2011193832A (en) * 2010-03-23 2011-10-06 Stanley Electric Co Ltd Lighting device for raising plant and apparatus for raising plant using the same
CN202112129U (en) * 2011-05-17 2012-01-18 北方民族大学 Culture shelf for virus-free potato foundation seedlings
CN103155860A (en) * 2011-12-09 2013-06-19 江阴沃土农业生物科技开发有限公司 Potato tissue culture frame
CN202374834U (en) * 2011-12-26 2012-08-15 哈尔滨商业大学 Plant tissue culture rack
CN202588022U (en) * 2012-06-01 2012-12-12 内蒙古凌志马铃薯科技股份有限公司 Cultivation frame
CN204426096U (en) * 2014-12-03 2015-07-01 凌云县长生仙草生物科技开发有限公司 A kind of energy-conservation culturing rack with fluorescent lamp
CN205389852U (en) * 2016-03-23 2016-07-27 连云港市农业科学院 Frame is cultivateed in plant illumination
JP2017195838A (en) * 2016-04-28 2017-11-02 昭和電工株式会社 Plant cultivation-lighting apparatus for and plant cultivating apparatus using the same

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