CN115141731A - Detector and operation method thereof - Google Patents

Abstract

A detector and an operation method thereof are provided, wherein the detector comprises a detection part placing and entering assembly, a detection part pressing assembly and a blending assembly. The detection component placing and entering and exiting assembly comprises a bearing table used for placing at least one detection component; the detection component pressing assembly is arranged on the first side of the detection component placing and entering and exiting assembly and is used for pressing at least one detection component; the blending subassembly sets up and places and pass in and out the first side of subassembly at the detection part for be connected with the detection part, with the sample in the blending detection part. The detection component placing and entering and exiting assembly is connected with the detection component pressing assembly and is configured to gradually press the detection component when the bearing table enters the detector. The detector can be matched with a detection part to realize full-automatic detection of a sample.

Description

Detector and operation method thereof

Technical Field

Embodiments of the present disclosure relate to a detector and a method of operating the same.

Background

The microfluidic technology is a technology for accurately controlling and controlling micro-scale fluid, and can integrate basic operation units such as samples, reaction, separation, detection and the like in the process of detection and analysis on a micro-nano-scale chip to automatically complete the whole analysis process. The microfluidic technology has the advantages of less sample consumption, high detection speed, simple and convenient operation, multifunctional integration, small volume, convenience for carrying and the like, and has great application potential in the fields of biology, chemistry, medicine and the like.

Disclosure of Invention

At least one embodiment of the present disclosure provides a detector, which includes a detection component placement and entry and exit assembly, a detection component compression assembly, and a blending assembly. The detection component placing and entering and exiting assembly comprises a bearing table used for placing at least one detection component; the detection component pressing assembly is arranged on a first side of the detection component placing and entering and exiting assembly and is used for pressing the at least one detection component; the blending subassembly sets up the first side that the detecting element was placed and was passed in and out the subassembly for with the detecting element is connected, in order to blend sample in the detecting element. The detection component placing and in-out assembly is connected with the detection component pressing assembly and is configured to gradually press the detection component by the detection component pressing assembly when the bearing table enters the detector.

For example, in the detecting apparatus provided in at least one embodiment of the present disclosure, the detecting component placing and entering assembly further includes a bracket and a first tray, the first tray is slidably disposed on the bracket, and the carrier is disposed on the first tray and configured to extend or enter the carrier into the detecting apparatus through sliding of the first tray.

For example, in the detector provided in at least one embodiment of the present disclosure, the detecting part placing and entering/exiting assembly further includes linear bearings that are disposed on the bracket and located at two sides of the first tray, the detecting part pressing assembly further includes a pressing plate, a guide shaft, a lower supporting plate, and a roller assembly, the guide shaft passes through the linear bearings, one end of the guide shaft is connected to the pressing plate, and the other end of the guide shaft is connected to the lower supporting plate; the side, facing the roller assembly, of the first tray is provided with a guide inclined plane at a position close to the linear bearing, the roller assembly is arranged on the lower supporting plate and comprises a roller support and a roller arranged on the roller support, and the roller is configured to slide along the guide inclined plane when the bearing table enters the detector, so that the lower supporting plate drives the guide shaft to move in the linear bearing, and the pressing plate is driven to gradually compress the detection part.

For example, in the detecting apparatus provided by at least one embodiment of the present disclosure, the detecting member pressing assembly further includes a spring, the spring is sleeved on the guide shaft, and the spring is configured to be compressed during the process that the pressing plate presses the detecting member.

For example, in the detector provided in at least one embodiment of the present disclosure, the detection component placement and entry/exit assembly further includes a photoelectric switch and a signal prompt circuit, and after the carrier is completely entered into the detector, an optical path of the photoelectric switch is blocked, the photoelectric switch is turned on, and the signal prompt circuit sends a prompt that the detection component is located at the origin.

For example, at least one embodiment of the present disclosure provides a testing apparatus, wherein a surface of the pressing plate facing the loading platform includes a puncturing mechanism configured to puncture a sealing film of a liquid storage cavity in the detection component when the pressing plate presses the detection component.

For example, at least one embodiment of the present disclosure provides a testing apparatus, wherein the testing member placement and access assembly further comprises a second tray, the bearing table is elastically connected to the second tray through a step shaft and a spring, so that the bearing table can move relative to the second tray in a direction perpendicular to the disc surface of the second tray; the second tray is arranged on the first tray in a sliding mode.

For example, in the detector provided in at least one embodiment of the present disclosure, the detection component placement and entry/exit assembly further includes a first guide rail, a first baffle, and a second baffle, where the first baffle and the second baffle are respectively disposed at two opposite ends of the second tray, and the second tray is slidably connected to the first tray through the first guide rail, and is limited by the first baffle and the second baffle.

For example, in the detecting apparatus provided by at least one embodiment of the present disclosure, the detecting member placing and entering assembly further includes a first motor, and a gear and a rack are fixed on an output shaft of the first motor, and configured to convert a rotational motion of the first motor into a linear motion of the first tray on the bracket.

For example, in the apparatus provided in at least one embodiment of the present disclosure, the homogenizing assembly includes at least one syringe pump, each of the at least one syringe pump includes a cylinder and a plunger rod, and the plunger rod moves in the cylinder to perform air suction or air release.

For example, in the detector provided by at least one embodiment of the present disclosure, the mixing component further includes a first support plate connected to the cylinder, a second support plate connected to the plunger rod, and a second guide rail, the first support plate and the second support plate are slidably disposed on the second guide rail, and at least one of the first support plate and the second support plate is configured to move on the second guide rail, so as to implement that the plunger rod moves in the cylinder.

For example, at least one embodiment of the present disclosure provides a measuring apparatus, wherein one end of the cylinder, which is close to the detecting member and is placed in and out of the assembly, includes a connecting assembly for connecting with the detecting member, and the connecting assembly includes a sealing ring for sealing the cylinder and the detecting member.

For example, at least one embodiment of the present disclosure provides that the apparatus further comprises a detection assembly for detecting the sample in the detection member.

For example, at least one embodiment of the present disclosure provides a meter in which the detection assembly includes an optical scanning assembly including at least one optical probe, each of the at least one optical probe including a light emission optical path and a light reception optical path for optically detecting a sample in the detection component.

For example, at least one embodiment of the present disclosure provides an apparatus wherein the optical scanning assembly further includes a conveyor belt, and the at least one optical probe includes a plurality of optical probes, and the plurality of optical probes are coupled to the conveyor belt and configured to move with the conveyor belt.

For example, at least one embodiment of the present disclosure provides a detector, wherein the light emitting optical paths of the plurality of optical probes are configured to emit light in different wavelength ranges.

For example, in the detecting apparatus provided by at least one embodiment of the present disclosure, the optical scanning assembly further includes a probe holder and a third guiding rail, the plurality of optical probes are disposed on the probe holder, a portion of the probe holder is connected to the conveyor belt, and another portion of the probe holder is connected to the third guiding rail, so that the plurality of optical probes can move along a track of the third guiding rail along with the conveyor belt.

For example, the detector provided in at least one embodiment of the present disclosure further includes: and the gas circuit assembly is arranged on a second side of the detection part placing and in and out assembly, the second side is opposite to the first side, and the gas circuit assembly is used for controlling the on-off of a flow channel in the detection part by applying air pressure.

For example, the detector provided in at least one embodiment of the present disclosure further includes: a magnet lift assembly disposed on a second side of the detection member placement and ingress and egress assembly, the second side opposite the first side, comprising a magnet, the magnet lift assembly configured to control movement of the magnet.

For example, in the detecting apparatus provided by at least one embodiment of the present disclosure, the magnet lifting assembly further includes a second motor, a fourth guide rail, and a magnet support, the magnet is disposed on the magnet support, the magnet support is slidably disposed on the fourth guide rail, and the second motor is configured to drive the magnet support to slide on the fourth guide rail.

For example, in the detecting apparatus provided in at least one embodiment of the present disclosure, the fourth guide rail is a linear guide rail and is disposed along a direction perpendicular to the table surface of the carrying table, so that the magnet holder slides on the fourth guide rail to approach or depart from the carrying table.

At least one embodiment of the present disclosure provides a method of operating a detector, the method including: controlling the detection component placing and in-and-out assembly to extend the bearing table out of the detector so as to place at least one detection component; controlling the detection component placement and in-and-out assembly to enable the bearing table to enter the detector, and enabling the detection component pressing assembly to gradually press the at least one detection component; and connecting the blending assembly with the at least one detection part to blend the sample in the detection part.

Drawings

To more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly introduced below, and it is apparent that the drawings in the following description relate only to some embodiments of the present disclosure and are not limiting to the present disclosure.

Fig. 1 is a schematic structural diagram of a detector provided in at least one embodiment of the present disclosure;

fig. 2 is another schematic structural diagram of a detector provided in at least one embodiment of the present disclosure;

FIG. 3 is a schematic diagram of a detection component placement and access assembly of a detector according to at least one embodiment of the present disclosure;

fig. 4 is a schematic structural diagram of a pressing assembly of a detection component in an inspection apparatus according to at least one embodiment of the present disclosure;

FIG. 5A is a schematic diagram illustrating a structure of a pressing plate of a pressing assembly of a detecting component in an inspection apparatus according to at least one embodiment of the present disclosure;

FIG. 5B is a schematic diagram illustrating a puncturing mechanism of a pressure plate of a test element pressing assembly of a testing machine according to at least one embodiment of the present disclosure;

FIG. 5C is a schematic cross-sectional view of a piercing mechanism of a pressure plate of a test element pressing assembly of a testing machine and a test element in accordance with at least one embodiment of the present disclosure;

fig. 6 is a schematic structural diagram of a blending component in the detector provided in at least one embodiment of the present disclosure;

fig. 7 is a schematic structural diagram of a detection assembly in a detector according to at least one embodiment of the present disclosure;

fig. 8 is a schematic structural diagram of a magnet lifting assembly in an inspection apparatus according to at least one embodiment of the present disclosure;

FIG. 9 is a top view of a base of a detection component provided in accordance with at least one embodiment of the present disclosure; and

fig. 10 is a bottom view of a base of a detection component provided in at least one embodiment of the present disclosure.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present disclosure more apparent, the technical solutions of the embodiments of the present disclosure will be described clearly and completely with reference to the drawings of the embodiments of the present disclosure. It is to be understood that the described embodiments are only a few embodiments of the present disclosure, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the disclosure without inventive step, are within the scope of protection of the disclosure.

Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this disclosure belongs. The use of "first," "second," and the like in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.

Generally, a detection component for detecting nucleic acid, such as a microfluidic chip, includes a sample chamber, a reagent chamber, a reaction chamber, a waste solution chamber, and a detection chamber, and is used for performing the whole process of sample loading, mixing, cleaning, and detection. However, due to the complexity and specificity of nucleic acid detection, it is often difficult for the detection components alone to achieve fully automated detection.

At least one embodiment of the present disclosure provides a detector, which includes a detection component placement and entry and exit assembly, a detection component compression assembly, and a blending assembly. The detection component placing and in-out assembly comprises a bearing table, a bearing table and a detection component, wherein the bearing table is used for placing at least one detection component; the detection component pressing assembly is arranged on the first side of the detection component placing and entering and exiting assembly and is used for pressing at least one detection component; the blending subassembly sets up and places and pass in and out the first side of subassembly at the detection part for be connected with the detection part, with the sample in the blending detection part. The detector can be matched with a detection part for use, and full-automatic detection of samples in the detection part is realized.

The meter of the present disclosure is illustrated by the following specific examples.

At least one embodiment of the present disclosure provides a detecting apparatus, and fig. 1 and fig. 2 respectively show schematic structural diagrams of the detecting apparatus viewed from different angles. As shown in fig. 1 and 2, the apparatus includes a detecting member placing and entering assembly 1, a detecting member pressing assembly 2, and a kneading assembly 4. In some embodiments, the monitor may further include a magnet lift assembly 3, a temperature cycling assembly 5, a detection assembly 6, an air path assembly 7, and a circuit control assembly 8. For example, the circuit control component 8 may be in communication connection with the above components to implement a control function for the components, thereby implementing a fully automatic detection.

The structure and function of the various components are described in detail below with reference to the figures.

For example, fig. 3 is a schematic structural diagram of a detection component placement and entry and exit assembly in a detector provided in at least one embodiment of the present disclosure. As shown in fig. 3, the inspection part placement and entry and exit assembly 1 includes a carrier stage 1-1 for placing at least one inspection part a, for example, a plurality of inspection parts a, four inspection parts a being shown as an example. For example, the detection part a may be a detection chip, such as a microfluidic chip.

For example, as shown in FIG. 3, the detecting member placing and moving-in/out assembly 1 further includes a support 1-5 (two supports 1-5 are shown as symmetrical in the figure) and a first tray 1-3, the first tray 1-3 is slidably disposed on the support 1-5, and the carrier table 1-1 is disposed on the first tray 1-3, and is configured to extend or enter the carrier table 1-1 into or out of the detecting apparatus by sliding the first tray 1-3 on the support 1-5, so as to place or remove the detecting member A and move the detecting member A to a designated position in the detecting apparatus.

For example, in some embodiments, the detection component placement and entry/exit assembly 1 may further include a photoelectric switch 1-9 and a signal prompting circuit (not shown), and the arrangement is such that after the carrier platform 1-1 completely enters the detection apparatus, the optical path of the photoelectric switch 1-9 is blocked, so that the photoelectric switch 1-9 is turned on, and the signal prompting circuit sends out a prompt to the origin of the detection component, thereby performing the function of locating the origin. For example, the optoelectronic switch 1-9 comprises an optoelectronic shutter, and after the carrier 1-1 enters the detector along with the first tray 1-3, the optoelectronic shutter is triggered to block the optical path of the optoelectronic switch 1-9, so as to open the optoelectronic switch 1-9.

For example, in the using process, when the detection component a needs to be placed in the detector, the bearing table 1-1 is pulled out, the detection component a is placed on the bearing table 1-1, then the bearing table 1-1 is pushed into the detector, at this time, the signal prompting circuit sends out a prompt that the detection component reaches the origin, and then the detector can be controlled to perform subsequent operations.

For example, in some embodiments, the bottom of the carrier 1-1 can be further provided with a detection switch (not shown) for detecting whether a detection component is placed. For example, the detection switch may be a photoelectric switch, when a detection component is placed in the carrier 1-1, the optical path of the photoelectric switch is blocked, and the photoelectric switch is turned on to prompt that a chip is placed, so as to realize a self-detection function of judging whether the detection component is placed.

For example, in some embodiments, the inspection part placement and access assembly 1 may further include a second tray 1-2, and the carrier 1-1 is elastically coupled to the second tray 1-2 by a step shaft and a spring (not shown) so that the carrier 1-1 can move relative to the second tray 1-2 in a direction perpendicular to the plane of the second tray 1-2 (i.e., up and down in the drawing). For example, the carrier 1-1 can be elastically connected to the second tray 1-2 by a combination of a plurality of step shafts and springs, for example, the springs are sleeved on the step shafts, so that the carrier 1-1 can move up and down relative to the second tray 1-2 under the condition of a force.

For example, the second tray 1-2 is slidably disposed on the first tray 1-3. For example, in some examples, the inspection part placement and access assembly 1 further includes a first guide rail 1-13, a first barrier 1-4, and a second barrier 1-10, the first barrier 1-4 and the second barrier 1-10 are respectively disposed at opposite ends of the second tray 1-2, and the second tray 1-2 is slidably coupled to the first tray 1-3 via the first guide rail 1-13 and is retained by the first barrier 1-4 and the second barrier 1-10.

For example, the first guide rail 1-13 is a linear guide rail, and in this case, the first barrier 1-4 and the second barrier 1-10 are located at both ends of the linear guide rail, so that the second tray 1-2 can move linearly on the first tray 1-3 and be limited by the first barrier 1-4 and the second barrier 1-10.

For example, the detecting member placing and moving in and out assembly 1 may further include a first motor 1-6, such as a stepping motor, and a gear and a rack 1-7 are fixed to an output shaft of the first motor 1-6, for example, by engagement of the gear and the rack, the rotational movement of the first motor 1-6 may be converted into the linear movement of the first tray 1-3 on the rack 1-5. Therefore, the automatic in-and-out of the bearing table 1-1 can be realized through the first motor 1-6.

For example, the supports 1-5 are provided with linear guides 1-8, and the first pallet 1-3 is slidably disposed on the linear guides 1-8, for example, as shown in fig. 3, the linear guides 1-8 are respectively disposed on the two supports 1-5, and the linear guides 1-8 can play a role of motion restriction, so that the first pallet 1-3 moves on the supports 1-5 along a track defined by the linear guides 1-8.

For example, the inspection component placement and access assembly 1 further includes base plates 1-12 for supporting the various structures of the inspection component placement and access assembly 1.

Compared with other detection component placement modes, the chip placement and access function realized by the detection component placement and access assembly 1 provided by the embodiment of the disclosure is simpler, safer and more convenient for interaction, and can also realize automation.

For example, fig. 4 is a schematic structural diagram of a pressing assembly of a detection component in a detector provided in at least one embodiment of the present disclosure. As shown in fig. 4, the detecting member pressing assembly 2 is disposed at a first side, i.e., an upper side as viewed in the drawing, of the detecting member placing and entering assembly 1, and presses at least one detecting member a to facilitate various operations to be subsequently performed on the detecting member a. The state shown in fig. 4 is a state when the detection part pressing assembly 2 presses the detection part a.

For example, as shown in fig. 1 and 2, the air passage assembly 7 is disposed on a second side (a lower side shown in the drawing) of the detection member placement and entrance and exit assembly 1, the second side being opposite to the first side, and is used for controlling the opening and closing of a flow passage in the detection member by applying air pressure (described in detail later). For example, the air path assembly 7 includes an air pump 7-1, an air path plate 7-2, and an air tank 7-3. The air pump 7-1 is used to effect the application of air pressure, such as the application of positive or negative pressure. The gas storage tank 7-3 is used for storing gas used when the gas pump 7-1 applies gas pressure. The air plate 7-2 is used for pressing with the detection component. For example, when the detection member pressing assembly 2 presses the detection member a, the lower side of the detection member a is pressed against a gas passage plate, which includes, for example, a plurality of vent holes or gas passages, which communicate with, for example, an on-off valve (hereinafter, a membrane valve portion, which can be turned on and off by air pressure) of a flow passage of the detection member, to form a gas passage sealed in the detection member and the gas passage plate, so that an air pump applies air pressure to the detection member a, thereby controlling the on and off of the flow passage in the detection member.

For example, in some embodiments, the inspection part placement and access assembly 1 is coupled to the inspection part compression assembly 2 and is configured such that the inspection part compression assembly 2 progressively compresses the inspection part as the carrier 1-1 enters the inspection apparatus.

For example, as shown in fig. 3 and 4, the detecting member placing and moving in and out assembly 1 further includes linear bearings 1-11 disposed on the brackets 1-5 and located at both sides of the first tray 1-3, as shown in fig. 4, the detecting member pressing assembly 2 further includes a pressing plate 2-1, a guide shaft 2-2, a lower plate 2-5, and a roller assembly 2-6, the guide shaft 2-2 passes through the linear bearings 1-11, one end (upper end in the drawing) of the guide shaft 2-2 is connected to the pressing plate 2-1, and the other end (lower end in the drawing) is connected to the lower plate 2-5. At a position close to the linear bearing 1-11, one side of the first tray 1-3 facing the roller assembly 2-6 is provided with a guide inclined surface 1-3-1, the roller assembly 2-6 is arranged on the lower supporting plate 2-5, and comprises a roller bracket and a roller arranged on the roller bracket, and the roller is configured to slide along the guide inclined surface 1-3-1 when the bearing platform 1-1 enters the detector (i.e. moves leftwards in the figure), so that the lower supporting plate 2-5 drives the guide shaft 2-2 to move (move downwards) in the linear bearing 1-11, and further drives the pressing plate 2-1 to gradually press the detection part A. In addition, when the bearing table 1-1 extends out of the detector (i.e. moves to the right in the figure), the roller slides along the guide inclined plane 1-3-1 so that the lower supporting plate 2-5 drives the guide shaft 2-2 to move (move upwards) in the linear bearing 1-11, and further drives the pressing plate 2-1 to gradually get away from the detection part A.

For example, as shown in fig. 4, the detecting member pressing assembly 2 may further include a spring 2-3, the spring 2-3 is sleeved on the guiding shaft 2-2, and the spring 2-3 is configured to be compressed during the pressing of the pressing plate 2-1 against the detecting member; in addition, in the process that the pressure plate 2-1 is far away from the detection part, the spring 2-3 can also provide restoring force so as to assist the pressure plate 2-1 to be gradually far away from the detection part A; in addition, the springs 2-3 can also provide a supporting function during the initial period (when the bearing platform 1-1 is pulled out of the detector), and the distance between the air channel plate 7-and the detection component is ensured during the initial period.

Compared with the pressing mode of other detection parts, the pressing mode provided by the embodiment of the disclosure does not need to be controlled by a single power source, and only needs to be placed with the detection part and linked with the in-and-out assembly 1, so that the debugging is facilitated, and the cost is saved.

For example, fig. 5A is a schematic structural diagram of a pressure plate in a pressing assembly of a detection component in an inspection apparatus according to at least one embodiment of the present disclosure. As shown in FIG. 5A, in some embodiments, the surface (lower surface in the figure) of the pressure plate 2-1 facing the carrier 1-1 comprises a puncturing mechanism 2-7, and the puncturing mechanism 2-7 is configured to puncture a sealing membrane of the reservoir in the detection part A when the pressure plate 2-1 presses the detection part A.

For example, fig. 5B is a schematic diagram illustrating a puncturing mechanism of a pressure plate of a test element pressing assembly in a testing machine according to at least one embodiment of the present disclosure. As shown in FIG. 5B, the pricking means 2-7 comprises a plunger 2-7-1, a needle-like projection 2-7-2 and a pressing portion 2-7-3, the needle-like projection 2-7-2 and the pressing portion 2-7-3 project from the surface of the plunger 2-7-1, and the projection length of the needle-like projection 2-7-2 exceeds the projection length of the pressing portion 2-7-3.

For example, the needle-like projection 2-7-2 is used to pierce the sealing film of the reservoir of the detection member, the needle-like projection 2-7-2 is located at the center of the top surface of the plunger 2-7-1, and the size of the needle-like projection 2-7-2 is smaller than the size of the opening of the reservoir. For example, the needle-like protrusions 2-7-2 may be triangular pyramids having a length of 3mm to 5mm, e.g., 4mm, and a top portion having a circular shape with a diameter of 1mm to 2mm, e.g., 1.5 mm.

For example, the pressing part 2-7-3 is plural, the plural pressing parts 2-7-3 are uniformly arranged along the circumferential direction of the needle-like projection 2-7-2, and the top surface of the pressing part 2-7-3 is a plane. For example, the pressing portions 2-7-3 may be bosses having a height of 1mm-3mm, for example, 2mm, and a number of, for example, 2-10, and arranged at equal intervals in the circumferential direction.

FIG. 5C is a schematic cross-sectional view of a piercing mechanism of a pressure plate of a test element pressing assembly of a testing machine and a test element in accordance with at least one embodiment of the present disclosure. As shown in FIG. 5C, in the process that the pressing plate 2-1 presses the detection member, the puncturing mechanism 2-7 acts on the reservoir A1 of the detection member, and the needle-like protrusions 2-7-2 are used to puncture the upper sealing film (not shown) of the reservoir A1 by the elastic member in the sealing cover A2. For example, as the puncturing mechanism 2-7 continues to move downward, the squeezing portion 2-7-3 contacts the periphery of the upper opening of the reservoir A1, and pushes the reservoir A1 to move downward. At this time, the plurality of squeezing portions 2-7-3 arranged at intervals can prevent the squeezing portions from blocking the opening of the liquid storage cavity A1, so that air can enter the liquid storage cavity A1 through gaps. For example, the lower end of the liquid storage cavity A1 is provided with another puncturing mechanism A3, when the liquid storage cavity A1 moves downwards to the puncturing mechanism A3, the puncturing mechanism A3 can puncture the lower sealing film A4 of the liquid storage cavity A1, and simultaneously the puncturing mechanisms 2-7 can puncture the upper sealing film A2 of the liquid storage cavity A1, so that the liquid in the liquid storage cavity A1 can flow out, for example, into a flow channel of the detection component and other functional cavities for subsequent operations. Therefore, in the process that the detection component pressing assembly 2 presses the detection component, the function of puncturing the sealing film of the liquid storage cavity of the detection component can be realized at the same time.

For example, fig. 6 is a schematic structural diagram of a blending component in a detector provided in at least one embodiment of the present disclosure. As shown in fig. 1 and 6, the kneading assembly 4 is provided on a first side (upper side in the drawing) of the detecting part placing and entering assembly 1 for connecting with the detecting part a to knead the sample in the detecting part a.

For example, as shown in FIG. 6, the blending assembly 4 includes at least one syringe pump, each of the at least one syringe pump including a cylinder 4-6 and a plunger rod 4-9, the plunger rod 4-9 being moved within the cylinder 4-6 to effect suction or deflation.

For example, for nucleic acid detection, the mixing assembly 4 can mix the sample and the magnetic beads in the detection component, which will be described in detail later.

For example, as shown in FIG. 6, the kneading assembly 4 further comprises a first support plate 4-5 connected to the cylinder 4-6, a second support plate 4-4 connected to the plunger rod 4-9, and a second guide rail 4-3, wherein the first support plate 4-5 and the second support plate 4-4 are slidably disposed on the second guide rail 4-3, and at least one of the first support plate 4-5 and the second support plate 4-4 is configured to move the plunger rod 4-9 in the cylinder 4-6 by moving on the second guide rail 4-3, i.e., one or both of the first support plate 4-5 and the second support plate 4-4 are controlled to move so as to generate a relative movement between the first support plate 4-5 and the second support plate 4-4, and further so that the plunger rod 4-9 can move relative to the cylinder 4-6, thereby exhausting or deflating air.

For example, the second guide rail 4-3 is a linear guide rail, so that the first support plate 4-5 and/or the second support plate 4-4 can move linearly on the second guide rail 4-3 to drive the cylinder 4-6 and/or the plunger rod 4-9 to move linearly, thereby realizing the air suction or air discharge of the plunger rod 4-9 through the movement in the cylinder 4-6.

For example, the blending assembly 4 may further include motors 4-7 and 4-8 for driving the first support plate 4-5 and the second support plate 4-4, respectively, such as stepper motors, 4-7 and 4-8 for driving the first support plate 4-5 and the second support plate 4-4, respectively, under control (e.g., under control of the circuit control assembly 8).

For example, the end of the cylinder 4-6 that is placed close to the sensing member and in and out of the assembly 1 includes a coupling assembly 4-10 for coupling with the sensing member A, the coupling assembly 4-10 including a sealing ring for sealing the cylinder with the sensing member. Therefore, the plunger rod 4-9 can move in the cylinder 4-6, for example, air suction or air discharge of the mixing cavity in the detection part A can be realized, and further mixing can be realized.

For example, the blending component 4 may further include a bracket 4-1 and a securing portion 4-2 for supporting and securing the above-described structure of the blending component 4.

For example, in some embodiments, the meter further comprises a detection assembly 6 for detecting a sample within the detection member. Fig. 7 is a schematic structural diagram of a detection assembly in a detector according to at least one embodiment of the present disclosure. As shown in fig. 7, the detection assembly 6 may be an optical scanning assembly, the optical scanning assembly 6 including at least one optical probe 6-1. For example, each optical probe 6-1 includes a light emission optical path and a light reception optical path (not shown) for optically detecting a sample in the detection section. For example, the light emitting optical path is used for emitting light to the sample in the detection component a, and the light receiving optical path is used for receiving light reflected by the sample, in which case, for example, detection and analysis of the sample can be realized by the characteristics of the reflected light or by comparing the difference between the reflected light and the light emitted by the light emitting optical path.

For example, the optical scanning assembly may further include a conveyor belt 6-4, the at least one optical probe 6-1 includes a plurality of optical probes 6-1, and the plurality of optical probes 6-1 are connected to the conveyor belt 6-4 and configured to move with the conveyor belt 6-1, such as to perform simultaneous optical scanning on a plurality of detection components. For example, the optical scanning assembly may further comprise a motor 6-2 (e.g., a stepper motor) and an idler 6-3, with the conveyor belt 6-4 being wound around the idler 6-3, and the motor 6-2 being adapted to drive the conveyor belt 6-4 under control (e.g., under control of the circuit control assembly 8).

For example, the light emitting paths of the plurality of optical probes 6-1 are configured to emit light in different wavelength ranges suitable for detecting different samples or different indexes. For example, each optical probe 6-1 can emit light in different wavelength ranges, such as four, six, or more wavelength ranges, suitable for detecting different samples or different indicators.

Therefore, the optical scanning assembly provided by the embodiment of the disclosure has a multi-channel optical scanning design, is strong in expansibility, and can realize multi-flux joint inspection.

For example, in some embodiments, the optical scanning assembly may further include a probe mount 6-7 and a third guide 6-5, the plurality of optical probes 6-1 being disposed on the probe mount 6-7, a portion of the probe mount 6-7 being coupled to the conveyor belt 6-4, another portion of the probe mount 6-7 being coupled to the third guide 6-5 to enable the plurality of optical probes 6-1 to move with the conveyor belt 6-4 along the trajectory of the third guide 6-5, whereby the third guide 6-5 may function to define the motion trajectory of the plurality of optical probes 6-1.

For example, the optical scanning assembly further includes a bracket 6-6 for carrying and supporting the above-described structure of the optical scanning assembly.

For example, fig. 8 is a schematic structural diagram of a magnet lifting assembly in a detecting instrument according to at least one embodiment of the present disclosure. As shown in fig. 8, the magnet lifting assembly 3 is disposed at a second side (lower side shown in the drawing) of the detecting member placing and entering assembly 1, and includes magnets 3-5, and the magnet lifting assembly 3 is configured to control movement of the magnets 3-5.

For example, for nucleic acid detection, the magnet in the magnet lifting assembly 3 can adsorb magnetic beads in the detection component when approaching the detection component, and release magnetic beads when departing from the detection component, thereby assisting in completing functions such as magnetic bead cleaning and magnetic bead mixing in the detection component.

For example, the magnet lifting assembly 3 further comprises a second motor 3-2 (e.g. a stepping motor), a fourth guide 3-3 and a magnet holder 3-4, the magnet 3-5 is disposed on the magnet holder 3-4, for example, the magnet 3-5 is elastically connected to the magnet holder 3-4 through a spring, the magnet holder 3-4 is slidably disposed on the fourth guide 3-3, and the second motor 3-2 is configured to drive the magnet holder 3-4 to slide on the fourth guide 3-3, for example, the direction and distance of sliding of the magnet holder 3-4 on the fourth guide 3-3 are controllable.

For example, the magnet lifting assembly 3 may include a corresponding number of magnet holders 3-4 and combinations of magnets 3-5, such as four magnets as shown in the figure, corresponding to a plurality of detection components on the platform 1-1, and the movement states of the four magnets 3-5 may be controlled to be the same, so as to ensure the consistency of the operation of the plurality of detection components; of course, in some embodiments, the movement states of the four magnets 3-5 may also be different to achieve different operations on the detection member.

For example, the fourth guide 3-3 is a linear guide and is disposed in a direction perpendicular to the top surface of the carrier 1-1 so that the magnet holder 3-4 slides on the fourth guide 3-3 to approach or separate from the carrier 1-1.

For example, the magnet lifting assembly 3 further includes a base 3-1 for securing and carrying the above-described structure of the magnet lifting assembly 3.

For example, the temperature cycle assembly 5 includes a heat conducting portion, a cooling portion, a heat radiating portion, and the like, and is at least used for realizing PCR (Polymerase Chain Reaction) temperature cycle and realizing Reaction and detection of nucleic acid.

For example, the circuit control assembly 8 is in communication connection with the above-mentioned components, that is, the detecting component placing and entering assembly 1, the detecting component pressing assembly 2, the magnet lifting assembly 3, the blending assembly 4, the temperature circulating assembly 5, the detecting assembly 6 and the air path assembly 7, in a wired or wireless manner, so as to control the above-mentioned operations of the above-mentioned components. For example, the circuit control assembly 8 may include any form of controller. For example, the controller may be various types of integrated circuit chips having processing functionality, which may have various computing architectures such as a Complex Instruction Set Computer (CISC) architecture, a Reduced Instruction Set Computer (RISC) architecture, or an architecture that implements a combination of instruction sets. In some embodiments, the controller 230 may be a microprocessor, such as an X86 processor or an ARM processor, or may be a digital processor (DSP), or the like.

For example, in some embodiments, the circuit control assembly 8 may further include a memory for storing control instructions and the like for implementing different tests, different procedures, and the like, by the test machine. For example, the storage unit may be any form of storage medium, such as a volatile memory or a nonvolatile memory, for example, a semiconductor memory or a magnetic medium memory, and the embodiments of the present disclosure are not limited thereto.

The detector provided by the embodiment of the disclosure can be realized as a full-automatic microfluidic detector of a nucleic acid detection part, has the functions of nucleic acid extraction, PCR temperature cycle, fluorescence detection and the like when detecting nucleic acid, and can realize the rapid and simple measurement process of sample inlet and result outlet. This detecting instrument should have the advantage of fast rising and falling temperature, real-time fluorescence detection, and be totally closed detecting system, has a plurality of optical detection passageways simultaneously, and can carry out the detection of a plurality of detection parts simultaneously, has the advantage of cost, space and time. In addition, a plurality of detectors can be used simultaneously to realize the expansion of channels, and in an application scene with larger detection flux, a plurality of detectors are adopted to form a detection system, so that the modularization of nucleic acid detection can be realized.

At least one embodiment of the present disclosure further provides an operating method of a detector, where the method includes: controlling the detection component placement and in-and-out assembly to enable the bearing table to extend out of the detector so as to place at least one detection component; controlling the detection component to be placed and enter and exit the assembly to enable the bearing table to enter the detector, and enabling the detection component pressing assembly to gradually press at least one detection component; and connecting the blending assembly with at least one detection part to blend the sample in the detection part.

A detection component (e.g., a detection chip) used with the detection apparatus provided by the embodiments of the present disclosure and an operation method of the detection apparatus used with the detection component are exemplarily described below with reference to the accompanying drawings.

Fig. 9 illustrates a top view of a substrate of a detection chip provided by at least one embodiment of the present disclosure. Fig. 10 illustrates a bottom view of a substrate of a detection chip provided in at least one embodiment of the present disclosure.

As shown in fig. 9 and 10, the detection chip includes a substrate 100, and the substrate 100 includes a sample chamber 110, an eluent chamber 180, a first washing solution chamber 150, a second washing solution chamber 160, a third washing solution chamber 170, a magnetic bead chamber 120, a first mixing chamber 130, a second mixing chamber 140, a waste solution chamber 190, and an amplification chamber 200; for example, a first lyophilizing chamber 102, a second lyophilizing chamber 103, and a plurality of substrate recesses 100c.

A plurality of fluid passages including a plurality of flow paths including a first flow path 1, a second flow path 2, a third flow path 3, a fourth flow path 4, a fifth flow path 5, a sixth flow path 6, a seventh flow path 7, an eighth flow path 8, a ninth flow path 9, a tenth flow path 10, and the like, and a plurality of membrane valve portions provided on part or all of the flow paths, respectively, are formed in the substrate 100. Further, the first flow path 1, the second flow path 2, the third flow path 3, the fourth flow path 4, the fifth flow path 5, the sixth flow path 6, the seventh flow path 7, the eighth flow path 8, the ninth flow path 9, and the tenth flow path 10 are provided therein with a first membrane valve section V1, a second membrane valve section V2, a third membrane valve section V3, a fourth membrane valve section V4, a fifth membrane valve section V5, a sixth membrane valve section V6, a seventh membrane valve section V7, an eighth membrane valve section V8, a ninth membrane valve section V9, and a tenth membrane valve section V10, respectively, which are configured to control communication and disconnection of corresponding at least part of the fluid channels so that the flow paths in which are present can be closed and opened, respectively.

Hereinafter, the operation steps of the detection apparatus for detecting nucleic acid in cooperation with a detection component (e.g., a detection chip such as a microfluidic chip) will be described in detail by way of a specific example.

Step S1: and (5) loading the sample.

A sample to be tested is added to the sample chamber 110 of the test element. A circuit control assembly 8 of the detector controls a first motor 1-6 of a detection component placing and in-out assembly 1 to drive a bearing platform 1-1 to extend out of the detector, a detection chip is placed on the bearing platform 1-1, then the first motor 1-6 is controlled to drive the bearing platform 1-1 to enter the detector, and at the moment, a detection component pressing assembly gradually presses the detection chip, so that operation components such as a blending assembly and the like are connected with the detection chip to perform subsequent operation. When the bearing table 1-1 completely enters the detector, the optical path of the photoelectric switch 1-9 for placing and entering the detecting component in the assembly 1 is shielded, the photoelectric switch 1-9 is opened, and the signal prompting circuit sends out a prompt that the detecting component reaches the origin.

Step S2: sample and lysis freeze-drying release step.

For example, the electrical control assembly 8 of the detector controls the air pump 7-1 of the air circuit assembly 7 to provide air pressure, e.g., negative pressure, to the first membrane valve portion V1 to open the first membrane valve portion V1, at which time the sample in the sample chamber 110 flows from the sample chamber 110 through the first flow path 1 first to the first lyophilization chamber 102, dissolves the lysate lyophilizate stored in the first lyophilization chamber 102, and then flows to the first homogenization chamber 130.

And step S3: magnetic bead release and (5) carrying out the following steps.

For example, the electrical control assembly 8 of the detection apparatus controls the air pump 7-1 of the air path assembly 7 to provide air pressure, such as negative pressure, to the second membrane valve portion V2 to open the second membrane valve portion V2, and at this time, the magnetic bead solution (i.e., the liquid containing magnetic beads) in the magnetic bead chamber 120 flows to the first mixing chamber 130 through the second flow path 2. Then, the electric circuit control assembly 8 controls the air pump of the air path assembly 7 to supply air pressure, for example, positive pressure, to the second membrane valve portion V2 to close the second membrane valve portion V2. At this time, the lysis solution, the sample, and the magnetic bead solution are mixed in the first mixing chamber 130.

And step S4: and (4) cracking and uniformly mixing.

For example, the circuit control component 8 of the detector controls the air pump of the air path component 7 to provide air pressure, for example, negative pressure, to the seventh membrane valve portion V7 to open the seventh membrane valve portion V7, then the circuit control component 8 controls the connection portion of the air cylinder 4-6 of the blending component 4 to be connected and sealed with the first blending cavity 130, and controls the plunger rod 4-9 to reciprocate in the air cylinder 4-6, so that the lysate, the sample and the magnetic bead solution are transported back and forth between the first blending cavity 130 and the second blending cavity 140 through the seventh flow path 7 to achieve blending.

The electronic control unit 8 then controls the air pump of the air circuit unit 7 to supply air pressure, such as positive pressure, to the seventh membrane valve unit V7 to close the seventh membrane valve unit V7.

Step S5: and (4) collecting and uniformly mixing magnetic beads.

For example, the circuit control assembly 8 of the detection apparatus controls the magnet lifting assembly 3 to move the magnets 3-5 to the bottom of the first mixing chamber 130, then the circuit control assembly 8 controls the air pump of the air path assembly 7 to provide air pressure, such as negative pressure, to the seventh membrane valve portion V7 to open the seventh membrane valve portion V7, then the circuit control assembly 8 controls the plunger rod 4-9 of the mixing assembly 4 to reciprocate in the cylinder 4-6, at this time, the mixed solution of the sample, the lysate and the magnetic beads reciprocates between the first mixing chamber 130 and the second mixing chamber 140 through the seventh flow path 7, finally the mixed solution without the magnetic beads stays in the second mixing chamber 140, the magnetic beads with the nucleic acid molecules adhered thereto stay in the first mixing chamber 130 due to the adsorption of the magnets, then the circuit control assembly 8 controls the magnet lifting assembly 3 to move the magnets 3-5 away from the first mixing chamber 130, and the circuit control assembly 8 controls the air pump of the air path assembly 7 to provide air pressure, such as positive pressure, to the seventh membrane valve portion V7 to close the seventh membrane valve portion V7.

Step S6: and (4) emptying the waste liquid.

For example, the electric circuit control unit 8 of the detecting instrument controls the air pump of the air path unit 7 to supply air pressure, for example, negative pressure, to the tenth membrane valve unit V10 to open the tenth membrane valve unit V10, and at this time, the mixed liquid without the magnetic beads flows as the waste liquid from the second kneading chamber 140 to the waste liquid chamber 190 through the tenth flow path 10, and then the circuit control unit 8 controls the air pump of the air path unit 7 to supply the air pressure, for example, the positive pressure, to the tenth membrane valve unit V10 to close the tenth membrane valve unit V10.

Step S7: and releasing the cleaning liquid.

For example, the circuit control component 8 of the detection apparatus controls the air pump of the air path component 7 to provide air pressure, for example, negative pressure, to the third membrane valve portion V3 to open the third membrane valve portion V3, at this time, the first cleaning solution in the first cleaning solution chamber 150 flows from the first cleaning solution chamber 150 to the first mixing chamber 130 through the third flow path 3 and redissolves the magnetic beads staying in the first mixing chamber 130, and then the circuit control component 8 controls the air pump of the air path component 7 to provide air pressure, for example, positive pressure, to the third membrane valve portion V3 to close the third membrane valve portion V3.

Step S8: and (5) drying the magnetic beads.

For example, the circuit control unit 8 of the detecting apparatus controls the heat conducting portion 5-1 of the temperature circulating unit 5 to heat the bottom of the first mixing chamber 130, and maintains the temperature at a constant temperature, for example, in the range of 30-70 ℃ for a certain time to dry the surfaces of the magnetic beads.

Step S9: and (4) releasing the eluent.

For example, the circuit control component 8 of the detection apparatus controls the air pump 7-1 of the air path component 7 to provide air pressure, for example, negative pressure, to the sixth membrane valve portion V6 to open the sixth membrane valve portion V6, at this time, the eluent in the eluent chamber 180 flows from the eluent chamber 180 to the first mixing chamber 130 through the sixth flow path 6 and re-dissolves the magnetic beads staying in the first mixing chamber 130, and then the circuit control component 8 controls the air pump 7-1 of the air path component 7 to provide air pressure, for example, positive pressure, to the sixth membrane valve portion V6 to close the sixth membrane valve portion V6.

Step S10: and (4) eluting and uniformly mixing.

For example, the circuit control assembly 8 of the detection apparatus controls the air pump 7-1 of the air path assembly 7 to provide air pressure, such as negative pressure, to the seventh membrane valve portion V7 to open the seventh membrane valve portion V7, then the circuit control assembly 8 controls the plunger rod 4-9 of the blending assembly 4 to reciprocate in the air cylinder 4-6, so that the mixed solution of eluent and magnetic beads reciprocates between the first blending chamber 130 and the second blending chamber 140 through the seventh flow path 7, and finally the mixed solution stays in the first blending chamber 130, and then the circuit control assembly 8 controls the air pump 7-1 of the air path assembly 7 to provide air pressure, such as positive pressure, to the seventh membrane valve portion V7 to close the seventh membrane valve portion V7 and control the heat conducting portion of the temperature circulating assembly 5 to stop heating.

Step S11: and (4) collecting and uniformly mixing magnetic beads.

For example, the circuit control assembly 8 of the detection apparatus controls the magnet lifting assembly 3 to move the magnets 3-5 to the bottom of the first mixing chamber 130, then the circuit control assembly 8 controls the air pump 7-1 of the air path assembly 7 to provide air pressure, such as negative pressure, to the seventh membrane valve portion V7 to open the seventh membrane valve portion V7, then the circuit control assembly 8 controls the plunger rod 4-9 of the mixing assembly 4 to reciprocate in the cylinder 4-6, so that the sample to be amplified moves back and forth between the first mixing chamber 130 and the second mixing chamber 140 through the seventh flow path 7, finally the sample to be amplified without magnetic beads stays in the second mixing chamber 140, and the magnetic beads without nucleic acid molecules stay in the first mixing chamber 130 due to the adsorption of the magnets, then the circuit control assembly 8 controls the magnet lifting assembly 3 to move the magnets 3-5 away from the first mixing chamber 130, and controls the air pump of the air path assembly 7 to provide air pressure, such as positive pressure, to the seventh membrane valve portion V7 to close the seventh membrane valve portion V7.

Step S12: a transfer step to the amplification chamber 200.

For example, the electronic control assembly 8 of the detection apparatus controls the air pump 7-1 of the air path assembly 7 to provide air pressure, e.g., negative pressure, to the eighth membrane valve portion V8 and the ninth membrane valve portion V9 to open the eighth membrane valve portion V8 and the ninth membrane valve portion V9, at which time, the sample to be amplified first flows to the second lyophilization chamber 103 through the eighth flow path 8 to reconstitute the amplified lyophilized in the second lyophilization chamber 103, and further flows to the amplification chamber 200. After filling the amplification chamber 200, the air flows to the air permeable chamber 400 through the ninth flow path 9, and then the electric circuit control assembly 8 controls the air pump 7-1 of the air circuit assembly 7 to supply air pressure, such as positive pressure, to the eighth membrane valve portion V8 and the ninth membrane valve portion V9 to close the eighth membrane valve portion V8 and the ninth membrane valve portion V9. Such a transfer step helps to fill the amplification chamber 200 with the sample to be amplified, and the excess elution solution containing nucleic acid molecules will stay in the eighth flow path 8, the ninth flow path 9 or the gas-permeable chamber 400.

Step S13: and (3) amplification and detection.

For example, the circuit control assembly 8 of the detector controls the heat conducting portion, the cooling portion and the heat radiating portion of the temperature cycling assembly 5 to perform PCR temperature cycling control on the amplification cavity so as to amplify the sample to be amplified therein, and then the circuit control assembly 8 controls the detection assembly 6 to detect the optical characteristics of the amplified sample so as to obtain the detection result. For example, the circuit control component 8 controls the conveyor belt 6-4 of the detection component 6 to move so as to drive the plurality of optical probes 6-1 to perform optical scanning, and controls the wavelength range of light emitted by the light emitting optical path of the optical probe 6-1 so as to realize detection of a certain index of the sample.

For example, the circuit control component 8 may obtain the detection result of the detection component 6 and save or upload the detection result to a cloud server.

Therefore, the nucleic acid can be fully automatically detected by matching the detector with the detection chip.

Of course, in other embodiments, the cooperation of the detecting component and the detecting instrument may also realize other simplified or more complicated operation steps, which is not specifically limited in the embodiments of the present disclosure.

The following points need to be explained:

(1) The drawings of the embodiments of the disclosure only relate to the structures related to the embodiments of the disclosure, and other structures can refer to the common design.

(2) For purposes of clarity, the thickness of layers or regions in the figures used to describe embodiments of the present disclosure are exaggerated or reduced, i.e., the figures are not drawn on a true scale.

(3) Without conflict, embodiments of the present disclosure and features of the embodiments may be combined with each other to arrive at new embodiments.

The above are only specific embodiments of the present disclosure, but the scope of the present disclosure is not limited thereto, and the scope of the present disclosure should be subject to the scope of the claims.

Claims (20)

1. A meter, comprising:

the detection component placement and access assembly comprises a bearing table, a detection component and a control component, wherein the bearing table is used for placing at least one detection component;

a detection component pressing assembly arranged on a first side of the detection component placing and entering-exiting assembly, for pressing said at least one detection member;

the blending assembly is arranged on the first side of the detection component placement and inlet and outlet assembly and is used for being connected with the detection component so as to blend the sample in the detection component;

the detection part placing and entering and exiting assembly is connected with the detection part pressing assembly and is configured to gradually press the detection part when the bearing table enters the detector.

2. The monitor of claim 1, wherein the test element placement and access assembly further comprises a support and a first tray slidably disposed on the support, the carrier being disposed on the first tray and configured to be extended or advanced into the monitor by sliding movement of the first tray.

3. The monitor of claim 1, wherein said test component placement and access assembly further comprises linear bearings disposed on said support and located on either side of said first tray,

the detection component pressing assembly further comprises a pressing plate, a guide shaft, a lower supporting plate and a roller assembly, the guide shaft penetrates through the linear bearing, one end of the guide shaft is connected with the pressing plate, and the other end of the guide shaft is connected with the lower supporting plate;

the side, facing the roller assembly, of the first tray is provided with a guide inclined plane at a position close to the linear bearing, the roller assembly is arranged on the lower supporting plate and comprises a roller support and a roller arranged on the roller support, and the roller is configured to slide along the guide inclined plane when the bearing table enters the detector, so that the lower supporting plate drives the guide shaft to move in the linear bearing, and the pressing plate is driven to gradually compress the detection part.

4. The testing machine of claim 3, wherein said testing member pressing assembly further comprises a spring disposed about said guide shaft and configured to be compressed during pressing of said pressing plate against said testing member.

5. The meter of claim 2, wherein the detection member placement and access assembly further comprises a photoelectric switch and a signal prompt circuit, wherein the photoelectric switch is configured such that when the carrier is fully inserted into the meter, the optical path of the photoelectric switch is blocked, the photoelectric switch is turned on, and the signal prompt circuit sends a prompt to the origin of the detection member.

6. The meter of claim 3, wherein a surface of the pressure plate facing the carrier includes a puncturing mechanism configured to puncture a sealing membrane of a reservoir within the detection member when the pressure plate is pressed against the detection member.

7. The monitor according to claim 6, wherein the puncturing mechanism includes an ejector rod, a needle-like projection and a pressing portion, the needle-like projection and the pressing portion project from a surface of the ejector rod, and a projecting length of the needle-like projection exceeds a projecting length of the pressing portion.

8. The inspection instrument of claim 2, wherein said inspection component placement and access assembly further comprises a second tray, said carrier being resiliently coupled to said second tray by a step shaft and a spring to enable movement of said carrier relative to said second tray in a direction perpendicular to a tray surface of said second tray;

the second tray is arranged on the first tray in a sliding mode.

9. The inspection instrument of claim 8, wherein said inspection component placement and access assembly further comprises a first rail, a first baffle, and a second baffle, said first baffle and said second baffle being disposed at opposite ends of said second tray, said second tray being slidably coupled to and retained by said first rail and said first tray.

10. The meter of claim 9, wherein the test element placement and access assembly further comprises a first motor,

a gear and a rack are fixed on an output shaft of the first motor, and the gear and the rack are configured to convert the rotary motion of the first motor into the linear motion of the first tray on the bracket.

11. The detector of claim 1, wherein the intermixing module comprises at least one syringe pump, each of the at least one syringe pump comprising a cylinder and a plunger rod that moves within the cylinder to effect aspiration or deflation.

12. The detector of claim 11, wherein the mixing assembly further comprises a first support plate connected to the cylinder, a second support plate connected to the plunger rod, and a second guide rail,

the first support plate and the second support plate are slidably disposed on the second guide rail, and at least one of the first support plate and the second support plate is configured to move on the second guide rail to realize the movement of the plunger rod in the cylinder.

13. The monitor of claim 12, wherein an end of the cylinder proximate the sensing member placement and access assembly includes a coupling assembly for coupling with the sensing member, the coupling assembly including a sealing ring for sealing the cylinder with the sensing member.

14. The meter of claim 1, further comprising a test assembly for testing a sample within the test member;

wherein the detection assembly comprises an optical scanning assembly comprising at least one optical probe, each of the at least one optical probe comprising a light emission optical path and a light reception optical path for optically detecting a sample in the detection component;

the optical scanning assembly further comprises a conveyor belt, the at least one optical probe comprising a plurality of optical probes connected to the conveyor belt and configured to move with the conveyor belt;

the light emitting optical paths of the plurality of optical probes are configured to emit light of different wavelength ranges.

15. The monitor of claim 14, wherein the optical scanning assembly further comprises a probe mount on which the plurality of optical probes are disposed, a portion of the probe mount being connected to the conveyor belt and another portion of the probe mount being connected to the third guide rail such that the plurality of optical probes are movable with the conveyor belt along a trajectory of the third guide rail.

16. The meter of claim 1, further comprising:

and the gas circuit assembly is arranged on a second side of the detection part placing and entering and exiting assembly, and the second side is opposite to the first side and used for controlling the on-off of a flow channel in the detection part by applying air pressure.

17. The meter of claim 1, further comprising:

a magnet lift assembly disposed on a second side of the detection member placement and ingress and egress assembly, the second side opposite the first side, comprising a magnet, the magnet lift assembly configured to control movement of the magnet.

18. The monitor of claim 17, wherein the magnet lift assembly further includes a second motor, a fourth guide rail, and a magnet support,

the magnet is arranged on the magnet support, the magnet support is arranged on the fourth guide rail in a sliding mode, and the second motor is configured to drive the magnet support to slide on the fourth guide rail.

19. The detector of claim 18, wherein the fourth guide is a linear guide and is arranged in a direction perpendicular to the table top of the carrier such that the magnet holder slides on the fourth guide to move closer to or further from the carrier.

20. A method of operating the meter of claim 1, comprising:

controlling the detection component placing and entering and exiting assembly to enable the bearing table to extend out of the detector so as to place at least one detection component;

controlling the detection component placement and in-and-out assembly to enable the bearing table to enter the detector, and enabling the detection component pressing assembly to gradually press the at least one detection component; and

and connecting the blending assembly with the at least one detection part to blend the sample in the detection part.

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