CN115141724A

CN115141724A - Stamp type strain inoculation sac for substance bacteriostasis experiment

Info

Publication number: CN115141724A
Application number: CN202210690569.XA
Authority: CN
Inventors: 吕欣怡
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-05-21
Filing date: 2022-05-21
Publication date: 2022-10-04
Anticipated expiration: 2042-05-21

Abstract

Seal formula bacterial inoculation bag for material bacteriostasis experiments belongs to biology technical field, comprises dipping in fungus liquid bag (1) and dipping in fungus liquid dish (8), its characterized in that: dip in fungus liquid bag (1) and dip in the fungus liquid of dipping in fungus liquid dish (8) and inoculate the inoculation bag on the culture plate after getting, dip in fungus liquid dish (8) and hold and treat that the fungus liquid confession of inoculating dips in the concave dish of fungus liquid of dipping in fungus liquid bag (1), utilize the outer wall utensil of flourishing water to dip in the abrupt thin-wall silica gel of fungus liquid bag membrane and dip in fungus liquid bag (1), dip in fungus liquid dish (8) and dip in the trace and treat the inoculation fungus liquid, then like the stamping, will treat that the inoculation fungus liquid inoculates on the culture plate of culture bacteria, finally reach and evenly with fungus liquid coating on the culture medium surface of culture plate, produce when preventing again the inoculation and stab the point or the pleasing to the eye purpose of culture plate when the mar influence is observed. The invention has the advantages of simple manufacture, strong operability, low cost and obvious effect.

Description

Stamp type strain inoculation sac for substance bacteriostasis experiment

Technical Field

The invention relates to a stamp type strain inoculation sac for substance bacteriostasis experiments, belonging to the technical field of biology.

Background

The main methods of bacterial inoculation used in microbial detection are five types: streaking, slant inoculation, stab inoculation, liquid and semi-solid inoculation, and spread inoculation. (1) plate scribing separation method: the method is characterized in that microbes mixed together or different cells in the same microbial population are encircled on the surface of a plate culture medium by an inoculating loop, a plurality of independently distributed single cells are obtained by zone streaking dilution, and the single cells grow and propagate into single colonies after culture, and the single colonies are generally regarded as pure species of the microbes to be separated. Sometimes, these single colonies are not all propagated from a single cell, and therefore, it is necessary to repeat the isolation several times to obtain pure species. The principle is that the microorganism sample is diluted from point to line on the surface of the solid culture medium for a plurality of times to achieve the separation purpose. In order to facilitate streaking, the culture medium should not be too thin, and about 20ml of the culture medium should be poured into each dish, so that the thickness of the culture medium is uniform and the surface of the plate is smooth. The scribing separation mainly comprises a partition scribing method and a continuous scribing method. The division scribing method is also called four-division scribing method because the flat plate is divided into four areas. During scribing, the flat plate is rotated by 60-70 degrees every time, and when the angle is changed every time, the bacteria on the inoculating loop are burned out, and then scribing is carried out at the previous scribing position; another continuous marking method is to continuously mark the other end of the plate from one point of the edge of the plate in a wave form without burning the bacteria on the inoculating loop. The continuous marking method is that the inoculating loop is burnt to be red through outside the alcohol lamp, the inoculating rod is also burnt fully, and finally the inoculating loop is burnt to be red in a centralized mode. Lightly shake the test tube to be inoculated, hold the lateral part of the bottom of the test tube to be inoculated by the heart of the left hand, hold the inoculating loop by the right hand, pull out the test tube plug by the little finger of the right hand, stretch the inoculating loop into the test tube near the alcohol burner, insert the inoculating loop into the inoculating liquid to be inoculated for a while, dip in the inoculating loop, take out the inoculating loop, burn the plug, cover the inoculating loop and put back the test tube rack. Or the inoculating loop is extended into the flat plate through a gap formed by slightly opening the dish cover, the inoculating loop is contacted with the blank of the edge of the flat plate to cool the inoculating loop, and then the inoculating loop is subjected to aseptic operation to directly take the bacterial colony to be separated and purified on the flat plate. Opening the flat dish cover at a position close to the alcohol lamp for about 30 degrees, extending the right hand ring into the flat dish, dibbling a strain at one position of a flat plate edge, lightly coating the edge of an agar culture medium, drawing out an inoculating ring, covering the flat dish cover, then burning redundant culture solution on the inoculating ring in flame, opening the flat dish cover for about 30 degrees, extending the inoculating ring, waiting for the inoculating ring to be cooled, lightly contacting with the inoculating liquid, starting to lightly slide and line on the flat plate surface, not embedding the inoculating ring into the culture medium, scratching the culture medium, enabling the lines to be parallel and dense, fully utilizing the flat plate surface area, paying attention to avoid overlapping the front line and the rear line, finishing the line marking, and closing the dish cover. Firing the inoculating loop, cooling and placing on an inoculating rack. The culture dish is placed in a thermostat with proper temperature upside down for culture so as to prevent the separated bacterial colony from being dispersed by the condensed water dropping from the dish cover during culture. After culturing, observing the colony shape growing along the streak on the streak flat plate, and inoculating the inclined plane after microscopic examination. The method for taking, inoculating and culturing the bacteria by the partition line drawing method is similar to the method for continuously drawing the lines. The partition scribing method is also called four-partition scribing method because the flat plate is divided into four regions during separation. The 4 th zone is the main distribution zone of single colony, so the area of the streaked line should be the largest. To prevent the contact between the scribe line in the 4 th region and the lines in the 1 st, 2 nd and 3 rd regions, the line in the 4 th region should be parallel to the line in the 1 st region, and the included angle between the region and the line in the region should preferably be kept about 120 °. When inoculating, firstly dipping a small amount of bacteria on the inoculating loop, dividing 3-5 parallel lines on the flat plate 1, taking out the inoculating loop, closing the dish cover with the left hand, rotating the flat plate by 60-70 degrees, burning off the redundant bacteria on the inoculating loop with the right hand, cooling the red inoculating loop on the edge of the flat plate, and then taking the bacteria marked on the area 1 as a bacteria source and carrying out the 2 nd parallel marking from the area 1 to the area 2 according to the method. After the 2 nd scribing is finished, the plate is rotated by about 60-70 degrees, and the scribing is also sequentially carried out on the 3 rd and 4 th regions. After the marking is finished, firing the inoculating ring, closing the dish cover, culturing by the same method, and observing a single colony in the marking area. (2) The slant inoculation method is mainly used for pure culture of single colony, preservation of strain or observation of certain characteristics of bacteria. The slant inoculation method comprises the following operation steps: (1) the left hand flatly holds two test tubes and the thumb presses the bottom of the test tube. The outer test tube is a strain test tube with bacterial lawn on the inclined surface, the inner test tube is a blank inclined surface to be inoculated, and the inclined surfaces of the two test tubes are upward at the same time. The test tube plugs were unscrewed with the right hand to allow easy extraction during inoculation. (2) The inoculating loop is held by the right hand like holding a writing brush, the end of the loop is burnt to be red and sterilized on flame, and then the rest parts which possibly extend into the test tube are also sterilized by fire. (3) The upper ends of the two test tubes are aligned and close to the flame, the test tube plugs of the two test tubes are clamped and pulled out together by using the little finger and the palm of the right hand, the test tube plugs are still clamped in the hand, and then the opening of the test tube slowly passes through the flame. Note that the test tube stopper should not be thrown on a table at will to be contaminated, and the test tube opening should not be burnt and scalded so as to avoid burst. (4) The burned inoculating loop is extended into the outside strain test tube. The tip of the inoculating loop is first contacted with the sterile medium to cool the loop if the procedure is rapid, at which point the inoculating loop is not completely cooled. And dipping a certain amount of lawn by using the inoculating loop according to the requirement, and paying attention to avoid scraping the culture medium. The inoculating loop with the lawn is quickly drawn out of the test tube, and the inoculating loop does not touch the tube wall or the tube opening. (5) The inoculating loop with the strain is quickly stretched into the bottom of another test tube with the inclined plane to be inoculated, and a straight line or a curve is slightly drawn upwards, so that the surface of the culture medium is not scratched. (6) The inoculated inclined test tube port is subjected to flame again, and the bottom of the test tube plug is plugged into the test tube immediately after the flame is passed. (7) Burning the inoculating loop with the lawn on flame for sterilization. Burning in inner flame to dry, and burning in outer flame to avoid pollution caused by bacteria sputtering due to sudden heat of bacterial coating. (8) After the ring is placed, the test tube plug is screwed, and a label is attached to the upper part of the outer surface of the test tube 2-3cm away from the test tube opening. (3) The puncture inoculation method is that an inoculation needle is used for sterilization by flame, a small amount of strain is dipped and vertically penetrates into the center of a test tube solid culture medium to the bottom, then the inoculation needle which picks up bacterial colony is inserted into the bottom of an agar slant surface in parallel with the tube wall, the needle is pulled out along the original inoculation line, a test tube plug and a test tube opening are burned, a test tube plug is plugged, and the residual thallus on the inoculation needle is burned on the flame. The hand needs to be steady and the action is light and quick. (4) The liquid inoculation method in the liquid and semi-solid inoculation methods includes inoculating the culture solution from an outside seed species or inoculating the liquid culture solution from a liquid seed species, and in both cases, inoculating with an inoculating loop is possible. However, in the case of a relatively large culture amount, the liquid inoculation is preferably performed by pipette, and aseptic operation is required. Holding the test tube with the left hand, stretching the burned inoculating loop into the strain tube with the right hand, cooling, taking the strain loop, immediately transferring the strain loop into the culture substrate tube, slightly grinding the strain loop on the tube wall close to the liquid level, slightly inclining the test tube, and taking a little liquid for blending to mix the strain liquid in the culture medium. The semi-solid culture medium inoculation method in the liquid and semi-solid inoculation methods is to insert a burning inoculating needle into a strain tube to be cooled, pick up a little strain liquid, immediately vertically insert the center of the semi-solid culture medium to a position close to the tube bottom but not directly insert the needle to the tube bottom, and then withdraw the culture along the original path. The nozzle is passed through flame, the cotton plug is plugged, and the inoculating needle is set down after burning and sterilization. The inoculated culture is cultured in a constant temperature box at 37 ℃, and the culture is taken out after 24 hours for observation. (5) The coating inoculation method is that a sterile pipette is used for sucking 0.1mL of bacterial suspension, the bacterial suspension is dripped on a culture medium flat plate, a sterile coating rod is held by the right hand, a culture dish is held by the left hand, a dish cover is opened by a thumb, bacterial liquid is uniformly coated and diffused from the center of the flat plate to the periphery of the flat plate by holding the coating rod by the right hand and the surface of the culture dish flat plate beside flame, and the bacterial liquid is prevented from being directly pushed to the edge of the flat plate or the culture medium is scratched by overexertion. After inoculation, the plates were placed upside down in an incubator and observed for culture. From the above, each inoculation method requires the use of a corresponding tool. Regarding an inoculation tool, the poplar and jade et al invented a self-adhesive plant disease inoculation paste with the application number of CN 200910074173.7. The device has a simple structure, is convenient to manufacture, and solves the problem of fixed-point quantitative inoculation in plant disease research. It is composed of two functional areas, a pasting area and an inoculation area. The sticking area is used for sticking to a plant body to realize a fixed point, and has the functions of moisture preservation and rain washing prevention. The inoculation area is provided with a bacterium tray bowl for placing the bacterium tray, so that the size of the bacterium tray can be controlled, and quantitative inoculation is realized. The adhesive sticker plant disease inoculation paste can be made into different shapes and sizes according to different inoculation positions and the size of the inoculation area of the plant. The invention of Shubin et al discloses a method of inoculating fungi, application No. CN201610326970.X, and inoculating apparatus and culture medium used in the method. The fungus inoculation method of the invention is that the inoculation tool which is made of metal and has a tip at one end and a wood or bamboo part is sterilized, the inoculation tool is transferred to a culture medium with target fungus in the inoculation tool under the aseptic environment and then the culture medium is cultured, the inoculation tool is transferred to the culture medium with the target fungus in the inoculation tool under the aseptic environment and then the culture medium is cultured, so that an inoculum formed by the wood or bamboo part on the inoculation tool is infected with the fungus, then the inoculation tool is taken out and the tip of the inoculation tool is punctured into the inoculation part of the host, so that the wood or bamboo inoculum with the infected fungus on the inoculation tool infects the host, and the inoculation operation is realized. The method has the characteristics of accuracy, convenience, time saving, labor saving and the like, and can inoculate harder parts such as woods and the like. People such as yellow gazang have invented a novel bacteria inoculator with the application number of CN202121194148.5, and the utility model relates to a novel bacteria inoculator, belonging to the technical field of experimental apparatus. The inoculation gun head is a cone, a spherical contact head for streak culture is arranged at the tip end of the inoculation gun head, a groove for scraping thalli is formed in the middle lower part of the inoculation gun head, a jack is formed in the large end of the cone, and one end of the operating handle is detachably inserted into the jack; the utility model has simple operation, and can avoid the damage of the culture medium during the streak culture of bacteria; overall structure is single, and the volume is narrow and small, can accomplish the sterilization work of inoculation instrument in batches in advance, ensures to carry out that enough aseptic inoculation rifle head can be changed when the inoculation is experimental, does benefit to the improvement of inoculation efficiency. The groove design is convenient for scraping a large amount of bacterial thalli at one time, and is beneficial to the rapid preparation of high-concentration bacterial suspension; the utility model discloses can repeat the sterilization after the washing and use, can reduce inoculation testing cost. At present, whether certain substances have antibacterial and bacteriostatic activity is generally verified by a round paper sheet method. The circular paper sheet method is that circular filter paper sheets are dipped in the aqueous solution of the substance to be verified and placed on a culture plate full of bacteria for culture, if the substance has antibacterial activity, transparent spots in a circular ring shape without bacteria growing appear around the circular paper sheets on the culture plate after a period of time, and the width of the circular ring indicates the height of the antibacterial activity. During verification, bacteriostatic activity of different concentrations can be verified, a plurality of culture plates are required to be operated and completed in each experiment, and microorganisms such as the same kind of bacteria are cultured on the plurality of culture plates. According to the traditional method and the traditional inoculation tool, a long time is usually needed from preparation, sterilization to inoculation culture of a plurality of culture plates, so that many people are tired and particularly the inoculation process is time-consuming, operators need to open, coat, mark, cover and seal a series of operations, particularly the coating process requires special care and high concentration, otherwise, a mark or scratch appears on the surfaces of the culture plates, therefore, how to quickly, efficiently and perfectly finish the inoculation work without uneven coating, the beauty of the culture plates becomes a big problem which needs to be solved urgently when observation is influenced by no mark or scratch on the surfaces of the culture plates, and finally, the bacteria liquid can be uniformly coated on the surfaces of the culture media of the culture plates, and the bacteria-inhibiting substances which can inhibit bacteria and can be used for the experiment are needed.

Disclosure of Invention

The invention provides a stamp type strain inoculation bag for substance bacteriostasis experiments, which utilizes a thin-wall silica gel bag with water-containing outer wall dipped with a bacterium solution bag membrane protrusion to dip a trace amount of bacterium solution to be inoculated in a bacterium solution dipping disc, and then inoculates the bacterium solution to be inoculated on a culture plate of the culture bacteria like stamping, thereby finally achieving the purposes of uniformly coating the bacterium solution on the surface of a culture medium of the culture plate and preventing the generation of stamp points or scratches during inoculation from influencing the attractiveness of the culture plate during observation.

The technical scheme adopted by the invention for solving the technical problems is as follows:

the invention relates to a stamp type strain inoculation bag for substance bacteriostasis experiments, which consists of a bacteria dipping liquid bag 1 and a bacteria dipping liquid disc 8, and is characterized in that: the bacterial liquid dipping bag 1 is an inoculation bag which is used for dipping bacterial liquid in a bacterial liquid dipping plate 8 and then inoculating the bacterial liquid onto a culture plate, and consists of a bag fixing plate 2, a handle 3, a water adding pipe 4, a handle cover 5, a handle cap 6, a connecting bolt 7, a bacterial liquid dipping bag membrane 13 and water 14, wherein the bacterial liquid dipping bag membrane 13 is a thin bag membrane which contains water at the lowest end of the bacterial liquid dipping bag 1 and is silica gel, the thickness of the thin bag membrane is 0.1-0.2 mm, the periphery of the bacterial liquid dipping bag membrane 13 is hermetically fixed on the periphery of the bag fixing plate 2, and the bacterial liquid dipping bag membrane 13 consists of a bacterial liquid dipping bag membrane body 15 and a bacterial liquid dipping bag membrane protrusion 16; the bacterial liquid dipping sac membrane body 15 is a main body of the bacterial liquid dipping sac membrane 13, the thickness of the bacterial liquid dipping sac membrane body 15 is 0.09-0.18 mm in a natural state, and the thickness of the bacterial liquid dipping sac membrane body 15 becomes thinner when the bacterial liquid dipping sac membrane body is under the action of the gravity of water 14; the bacterial liquid dipping sac membrane protrusions 16 are hexagonal bulges distributed on the outer surface of the bacterial liquid dipping sac membrane body 15, the side length of each hexagonal bacterial liquid dipping sac membrane protrusion 16 is 1-5 micrometers, the thickness of each bacterial liquid dipping sac membrane protrusion 16 is 0.01-0.02 millimeter, and the distance between every two adjacent bacterial liquid dipping sac membrane protrusions 16 is 1-5 micrometers; after water 14 is poured into the bacteria dipping liquid sac 1, the bacteria dipping liquid sac membrane 13 can droop by 1-2 cm due to the action of gravity; the capsule fixing plate 2 is a round plate for fixing the capsule membrane 13 of the dipped bacteria liquid, is made of rubber, plastic, copper, stainless steel or aluminum alloy, has the thickness of 2-5 mm and the diameter of 2-10 cm, is fixed at the lower end of the handle 3 on the upper surface and is connected with the handle 3 into a whole; the handle 3 is a holding structure above the bag fixing plate 2, the lower end of the handle is connected with the bag fixing plate 2, the upper end of the handle is free, the outer surface of the upper end of the handle is provided with a spiral wire, the inner surface of the handle cap 6 is also provided with a spiral wire, and the spiral wire on the outer surface of the upper end of the handle 3 and the spiral wire on the inner surface of the handle cap 6 form a connecting bolt 7; the water adding pipe 4 is a central pipeline of the handle 3, the upper end of the water adding pipe 4 is thick in pipe diameter and is funnel-shaped, the diameter of a cross section circle at the upper end of the funnel is 0.5-1 cm, the diameter of the cross section circle at the upper end of the funnel is 0.2-0.5 cm, the height of the funnel is 1-2 cm, the lower part of the water adding pipe 4 is thin and cylindrical in pipe diameter, the diameter of the cross section circle of the cylinder is 0.2-0.5 cm, and the lower end of the water adding pipe 4 is communicated with an inner cavity which is formed by the bacteria dipping sac membrane 13 and the sac fixing plate 2 and is used for containing water 14; the handle cover 5 is made of rubber, plastic, copper, stainless steel or aluminum alloy, the upper half part of the handle cover 5 is cylindrical, the diameter of the cylinder is 1-3 cm, and the height of the cylinder is 0.5-1 cm; the lower half part of the handle cover 5 is a handle cap 6, the handle cap 6 is cylindrical, and the inner surface of the handle cap is provided with a spiral thread engaged with the spiral thread on the outer surface of the upper end of the handle 3.

The bacteria dipping liquid disc 8 is a concave disc for containing bacteria liquid to be inoculated for the bacteria dipping liquid bag 1 to dip the bacteria liquid, is made of ceramic, glass, stainless steel, copper or aluminum alloy, and consists of a bacteria dipping liquid disc cavity 9, a bacteria dipping liquid disc wall 10, a disc handle 11 and a base 12; the bacteria dipping plate cavity 9 is a concave cavity surrounded by bacteria dipping plate walls 10 at the top of the bacteria dipping plate 8; the bacteria liquid dipping plate wall 10 is positioned at the top of the bacteria liquid dipping plate 8, is spherical and has the thickness of 1-5 mm; the diameter of a circular opening on the upper edge of the bacteria dipping liquid disk wall 10 is 15-20 cm, the maximum depth of the bacteria dipping liquid disk wall 10 is 1 cm, and the inner surface of the bacteria dipping liquid disk wall 10 is smooth; the tray handle 11 is a connecting part, the upper end of the connecting part is connected to the center of the outer surface of the bottom of the bacteria dipping liquid tray wall 10, the lower end of the connecting part is connected to the center of the upper surface of the base 12, the height of the tray handle 11 is 3-5 cm, the cross section of the tray handle is circular, the diameter of the cross section of the upper end of the tray handle 11 is 0.5-1 cm, and the diameter of the cross section of the lower end of the tray handle 11 is 10-15 cm; the base 12 is a structural component at the lower part of the bacteria dipping liquid disk 8 and is cylindrical, and the diameter of the cylindrical base 12 is 10-15 cm, and the height of the cylindrical base is 1-2 cm.

The seal type strain inoculation bag for the substance bacteriostasis experiment has the beneficial effects that a small amount of strain liquid to be inoculated is dipped in a strain liquid dipping disk by utilizing a thin-wall silica gel bag which is filled with water and provided with a strain liquid bag membrane protrusion on the outer wall, and then the strain liquid to be inoculated is inoculated to a culture plate of the culture bacteria like stamping, so that the aims of uniformly coating the strain liquid on the surface of a culture medium of the culture plate and preventing poking points or scratches generated during inoculation from influencing the appearance of the culture plate during observation are fulfilled. The invention has the advantages of simple manufacture, strong operability, low cost and obvious effect.

Drawings

The invention is further described below with reference to the accompanying drawings.

FIG. 1 is a schematic diagram of the whole structure of a dip bacterial sac of a stamp type bacterial inoculation sac for substance bacteriostasis experiments.

FIG. 2 is a schematic view of the whole structure of a dip plate for a stamp type bacterial strain inoculation sac for substance bacteriostasis experiments.

FIG. 3 is a schematic diagram of a section structure of a membrane of a stamp type bacterial strain inoculation sac for a substance bacteriostasis experiment.

In the figure, 1, a bacteria dipping liquid sac, 2, a sac fixing plate, 3, a handle, 4, a water adding pipe, 5, a handle cover, 6, a handle cap, 7, a connecting bolt, 8, a bacteria dipping liquid disc, 9, a bacteria dipping disc cavity, 10, a bacteria dipping disc wall, 11, a disc handle, 12, a base, 13, a bacteria dipping liquid sac membrane, 14, water, 15, a bacteria dipping liquid sac membrane body and 16, bacteria dipping liquid sac membrane protrusions are arranged.

Detailed Description

The first embodiment is as follows:

as shown in the figure, the stamp type strain inoculation bag for substance bacteriostasis experiments comprises a strain dipping liquid bag 1, a bag fixing plate 2, a handle 3, a water adding pipe 4, a handle cover 5, a handle cap 6, a connecting bolt 7, a strain dipping liquid disk 8, a strain dipping liquid disk cavity 9, a strain dipping liquid disk wall 10, a disk handle 11, a base 12, a strain dipping liquid bag membrane 13, water 14, a strain dipping liquid bag membrane body 15 and a strain dipping liquid bag membrane protrusion 16. The bacterial liquid dipping bag 1 is an inoculation bag which is used for dipping bacterial liquid in a bacterial liquid dipping disk 8 and then inoculating the bacterial liquid onto a culture plate and consists of a bag fixing plate 2, a handle 3, a water adding pipe 4, a handle cover 5, a handle cap 6, a connecting bolt 7, a bacterial liquid dipping bag membrane 13 and water 14, when the bacterial liquid dipping bag is used, an operator holds the handle to enable the bacterial liquid dipping bag membrane 13 of the bacterial liquid dipping bag 1 to be placed under a bacterial liquid dipping disk cavity 9 pier 2-5 in which the bacterial liquid to be inoculated is dripped, then the bacterial liquid dipping disk cavity is picked up, a small amount of the bacterial liquid to be inoculated can be adhered to the outer surface of the bacterial liquid dipping bag membrane 13, then the bacterial liquid to be cultured is lightly placed on the culture plate in a seal mode, after the bacterial liquid to be inoculated is picked up, the bacterial liquid to be uniformly coated on the surface of the culture plate, the situation that the bacteria on the surface of the inoculated culture plate can be uniformly grown is avoided, the bacteria colony with uniform growth is provided, and the bacteria inhibition effect is improved; the dipping bacterium solution capsule membrane 13 is a thin capsule membrane which is used for containing water at the lowest end of the dipping bacterium solution capsule 1, is silica gel, is 0.1-0.2 mm thick, is hermetically fixed at the periphery of the capsule fixing plate 2 at the periphery of the dipping bacterium solution capsule membrane 13, and consists of a dipping bacterium solution capsule membrane body 15 and a dipping bacterium solution capsule membrane protrusion 16, wherein the periphery of the dipping bacterium solution capsule membrane 13 is fixedly sealed at the periphery of the capsule fixing plate 2; the bacterial liquid dipping sac membrane body 15 is a main body of the bacterial liquid dipping sac membrane 13, and the thickness of the bacterial liquid dipping sac membrane body 15 is 0.09-0.18 mm in a natural state, so that the bacterial liquid dipping sac membrane body becomes thinner under the action of the gravity of water 14; the bacterial liquid dipping capsule membrane protrusions 16 are hexagonal protrusions distributed on the outer surface of the bacterial liquid dipping capsule membrane body 15, the side length of each hexagonal bacterial liquid dipping capsule membrane protrusion 16 is 1-5 micrometers, the thickness of each bacterial liquid dipping capsule membrane protrusion 16 is 0.01-0.02 millimeter, the distance between every two adjacent bacterial liquid dipping capsule membrane protrusions 16 is 1-5 micrometers, and the bacterial liquid dipping capsule membrane protrusions 16 are arranged to enable the outer surface of the bacterial liquid dipping capsule membrane 13 to be rough so as to better dip bacterial liquid to be inoculated; after water 14 is filled into the bacteria dipping liquid bag 1, the bacteria dipping liquid bag membrane 13 can droop by 1-2 cm under the action of gravity, so that when bacteria are dipped, the bacteria dipping liquid bag membrane 13 can be tightly pressed with the bacteria dipping liquid disk wall 10, the bacteria to be inoculated are uniformly filled in a gap between the outer surface of the bacteria dipping liquid bag membrane body 15 and the inner surface of the bacteria dipping liquid disk wall 10, the use amount of the bacteria is reduced, and a tiny drop of bacteria can be inoculated to a plurality of culture plates; the capsule fixing plate 2 is a circular plate for fixing the capsule dipping membrane 13 and is made of rubber, plastic, copper, stainless steel or aluminum alloy, the thickness of the capsule fixing plate 2 is 2-5 mm, the diameter of the capsule fixing plate is 2-10 cm, the upper surface of the capsule fixing plate is fixed at the lower end of the handle 3 and is connected with the handle 3 into a whole, when the capsule dipping membrane 13 is fixed, the periphery of the capsule dipping membrane 13 is adhered to the periphery of the capsule fixing plate 2 by glue, and then the capsule dipping membrane 13 is tightly bound by iron wires or iron sheets, so that the capsule fixing plate 2 can be hermetically fixed with the capsule dipping membrane 13; the handle 3 is a holding structure above the bag fixing plate 2, the lower end of the handle is connected with the bag fixing plate 2, the upper end of the handle is free, the outer surface of the upper end of the handle is provided with a spiral wire, the inner surface of the handle cap 6 is also provided with a spiral wire, the spiral wire on the outer surface of the upper end of the handle 3 and the spiral wire on the inner surface of the handle cap 6 jointly form a connecting bolt 7, and the connecting bolt 7 is used for being screwed with the spiral wire on the inner surface of the handle cap 6 to prevent poured water 14 from overflowing; the water adding pipe 4 is a central pipeline of the handle 3, the upper end of the water adding pipe 4 is thick in pipe diameter and is funnel-shaped, the diameter of a cross section circle at the upper end of the funnel is 0.5-1 cm, the diameter of the cross section circle at the upper end of the funnel is 0.2-0.5 cm, the height of the funnel is 1-2 cm, the lower part of the water adding pipe 4 is thin and cylindrical, the diameter of a cross section circle of the cylinder is 0.2-0.5 cm, and the lower end of the water adding pipe 4 is communicated with an inner cavity formed by the bacterial dipping liquid sac membrane 13 and the solid sac plate 2 and used for adding water 14 into the inner cavity formed by the bacterial dipping liquid sac membrane 13 and the solid sac plate 2 and used for containing the water 14; the handle cover 5 is a structure which is formed by adding water 14 into an inner cavity which is formed by the bacteria dipping liquid sac membrane 13 and the sac fixing plate 2 and used for containing the water 14, the upper end of the handle 3 is sealed, the structure is made of rubber, plastics, copper, stainless steel or aluminum alloy, the upper half part of the handle cover 5 is cylindrical, the diameter of the cylinder is 1-3 cm, and the height of the cylinder is 0.5-1 cm; the lower half part of the handle cover 5 is a handle cap 6, the handle cap 6 is cylindrical, the inner surface of the handle cap is provided with a spiral wire meshed with the spiral wire on the outer surface of the upper end of the handle 3, and the water adding pipe 4 can be sealed after the handle cover 5 is tightly screwed on the upper end of the handle 3 so as to prevent the water 14 from overflowing. The bacteria dipping liquid disc 8 is a concave disc for holding bacteria liquid to be inoculated for the bacteria dipping liquid bag 1 to dip the bacteria liquid, is made of ceramic, glass, stainless steel, copper or aluminum alloy, and consists of a bacteria dipping liquid disc cavity 9, a bacteria dipping liquid disc wall 10, a disc handle 11 and a base 12; the bacteria liquid dipping plate cavity 9 is a concave cavity surrounded by bacteria liquid dipping plate walls 10 at the top of the bacteria liquid dipping plate 8; the bacteria liquid dipping plate wall 10 is positioned at the top of the bacteria liquid dipping plate 8, is spherical and has the thickness of 1-5 mm; the diameter of a circular opening on the upper edge of the bacteria dipping liquid disk wall 10 is 15-20 cm, the maximum depth of the bacteria dipping liquid disk wall 10 is 1 cm, and the inner surface of the bacteria dipping liquid disk wall 10 is smooth; the disc handle 11 is a connecting component with the upper end connected to the center of the outer surface of the bottom of the bacteria liquid dipping disc wall 10 and the lower end connected to the center of the upper surface of the base 12 and is used for picking up or moving the bacteria liquid dipping disc 8, the height of the disc handle 11 is 3-5 cm, the cross section is circular, the diameter of the cross section of the upper end of the disc handle 11 is 0.5-1 cm, and the diameter of the cross section of the lower end of the disc handle 11 is 10-15 cm; the base 12 is a structural component at the lower part of the bacterial liquid dipping disc 8 and is cylindrical, and the diameter of the cylindrical base 12 is 10-15 cm, and the height is 1-2 cm; during the use, will dip in the fungus liquid dish 8 sterilization in advance, then arrange in the superclean bench in, treat the inoculation fungus liquid with the pipette absorption, blow in and dip in fungus liquid dish chamber 9, if the culture plate of inoculation is more, can dip in fungus liquid dish 8 by one hand, another hand is dipped in fungus liquid bag 1, like the stamp, inoculate the fungus liquid on the culture plate fast, owing to need not ball or setting-out, when needing a plurality of culture plate cultivateing the fungus, can accomplish inoculation work fast high-efficiently.

The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined by the appended claims and their equivalents.

Claims

1. Seal formula bacterial inoculation bag for material bacteriostasis experiments comprises dipping liquid fungus bag (1) and dipping liquid fungus dish (8), its characterized in that: the bacterial liquid dipping bag (1) is an inoculation bag which is used for dipping bacterial liquid in a bacterial liquid dipping plate (8) and then inoculating the bacterial liquid onto a culture plate and consists of a solid bag plate (2), a handle (3), a water adding pipe (4), a handle cover (5), a handle cap (6), a connecting bolt (7), a bacterial liquid dipping bag membrane (13) and water (14), wherein the bacterial liquid dipping bag membrane (13) is a thin bag membrane which contains water at the lowest end of the bacterial liquid dipping bag (1) and is silica gel, the thickness of the bacterial liquid dipping bag membrane is 0.1-0.2 mm, the periphery of the bacterial liquid dipping bag membrane (13) is hermetically fixed on the periphery of the solid bag plate (2), and the bacterial liquid dipping bag membrane (13) consists of a bacterial liquid dipping bag membrane body (15) and a bacterial liquid dipping bag membrane protrusion (16); the bacterial solution dipping sac body (15) is a main body of the bacterial solution dipping sac body (13), the thickness of the bacterial solution dipping sac body (15) is 0.09-0.18 mm in a natural state, and the bacterial solution dipping sac body (15) becomes thinner under the action of the gravity of water (14); the bacterial fluid dipping sac membrane protrusions (16) are hexagonal protrusions distributed on the outer surface of the bacterial fluid dipping sac membrane body (15), the side length of each hexagonal bacterial fluid dipping sac membrane protrusion (16) is 1-5 micrometers, the thickness of each bacterial fluid dipping sac membrane protrusion (16) is 0.01-0.02 millimeter, and the distance between every two adjacent bacterial fluid dipping sac membrane protrusions (16) is 1-5 micrometers; after water (14) is poured into the bacterial liquid dipping sac (1), the bacterial liquid dipping sac membrane (13) can droop by 1-2 cm under the action of gravity; the capsule fixing plate (2) is a round plate for fixing a capsule membrane (13) dipped with bacteria liquid and is made of rubber, plastic, copper, stainless steel or aluminum alloy, the thickness of the capsule fixing plate (2) is 2-5 mm, the diameter of the capsule fixing plate is 2-10 cm, the upper surface of the capsule fixing plate is fixedly arranged at the lower end of the handle (3) and is connected with the handle (3) into a whole; the handle (3) is a holding structure above the bag fixing plate (2), the lower end of the handle is connected with the bag fixing plate (2), the upper end of the handle is free, the outer surface of the upper end of the handle is provided with a spiral wire, the inner surface of the handle cap (6) is also provided with a spiral wire, and the spiral wire on the outer surface of the upper end of the handle (3) and the spiral wire on the inner surface of the handle cap (6) form a connecting bolt (7) together; the water adding pipe (4) is a central pipeline of the handle (3), the upper end of the water adding pipe (4) is thick in pipe diameter and is funnel-shaped, the diameter of a cross section circle at the upper end of the funnel is 0.5-1 cm, the diameter of the cross section circle at the upper end of the funnel is 0.2-0.5 cm, the height of the funnel is 1-2 cm, the lower part of the water adding pipe (4) is thin and cylindrical, the diameter of the cross section circle of the cylinder is 0.2-0.5 cm, and the lower end of the water adding pipe (4) is communicated with an inner cavity for containing water (14) formed by the bacterial liquid dipping sac membrane (13) and the solid sac plate (2); the handle cover (5) is made of rubber, plastic, copper, stainless steel or aluminum alloy, the upper half part of the handle cover (5) is cylindrical, the diameter of the cylinder is 1-3 cm, and the height of the cylinder is 0.5-1 cm; the lower part of the handle cover (5) is a handle cap (6), the handle cap (6) is cylindrical, and the inner surface of the handle cap is provided with a spiral thread meshed with the spiral thread on the outer surface of the upper end of the handle (3).

2. The stamp-type strain inoculation sac for substance bacteriostasis experiments, according to claim 1, is characterized in that: the bacteria liquid dipping disc (8) is a concave disc for containing bacteria liquid to be inoculated for the bacteria liquid dipping bag (1) to dip the bacteria liquid, is made of ceramic, glass, stainless steel, copper or aluminum alloy, and consists of a bacteria liquid dipping disc cavity (9), a bacteria liquid dipping disc wall (10), a disc handle (11) and a base (12); the bacteria dipping plate cavity (9) is a concave cavity surrounded by bacteria dipping plate walls (10) at the top of the bacteria dipping plate (8); the bacteria liquid dipping plate wall (10) is positioned at the top of the bacteria liquid dipping plate (8), is spherical and has the thickness of 1-5 mm; the diameter of a circular opening on the upper edge of the bacteria dipping liquid disk wall (10) is 15-20 cm, the maximum depth of the bacteria dipping liquid disk wall (10) is 1 cm, and the inner surface of the bacteria dipping liquid disk wall (10) is smooth; the upper end of the tray handle (11) is connected to the center of the outer surface of the bottom of the bacteria liquid dipping tray wall (10), the lower end of the tray handle is connected to the center of the upper surface of the base (12), the height of the tray handle (11) is 3-5 cm, the cross section of the tray handle is circular, the diameter of the cross section of the upper end of the tray handle (11) is 0.5-1 cm, and the diameter of the cross section of the lower end of the tray handle is 10-15 cm; the base (12) is a structural component at the lower part of the bacteria dipping liquid disc (8) and is cylindrical, and the diameter of the cylindrical base (12) is 10-15 cm, and the height of the cylindrical base is 1-2 cm.