CN1151175C - Method for extracting tremella mesenterica polysaccharide - Google Patents
Method for extracting tremella mesenterica polysaccharide Download PDFInfo
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- CN1151175C CN1151175C CNB021126917A CN02112691A CN1151175C CN 1151175 C CN1151175 C CN 1151175C CN B021126917 A CNB021126917 A CN B021126917A CN 02112691 A CN02112691 A CN 02112691A CN 1151175 C CN1151175 C CN 1151175C
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- polysaccharide
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- ultrafiltration
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- tremella mesenterica
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Abstract
The present invention relates to a method for extracting main components from tremella aurantia fungui, which belongs to the technical field of health food and medicines. The method comprises the steps of separating hyphae in a pot, steaming hyphae, hydrolyzing steaming liquor by strong alkali, centrifugally separating and super filtering hydrolysate, concentrating filtrate by alcohol, crystallizing the filtrate, etc. The present invention provides a practicable method for extracting tremella aurantia polysaccharide. The main component, namely the tremella aurantia polysaccharide, of tremella aurantia, is simply and conveniently obtained by using the method. Therefore, the industrial development and utilization of a special natural plant, namely the tremella aurantia, in China becomes possible.
Description
Technical field
The present invention relates to extraction of effective components in a kind of fungi, the extracting method of main component belongs to protective foods, field of pharmaceutical technology in the especially golden ear fungi.
Background technology
The gold ear is a food medicine fungi famous in Basidiomycetes, the Tremellaceae, mainly originates in the remote mountains on ground such as Yunnan Province of China, Guizhou.Put down in writing according to Compendium of Material Medica: Jin Er can control " the addiction drink gathers, the stomachache metal-inflicted wound ".Its flavor efficacy has literary composition to carry " band is cold in the temperature, and it is sweet to distinguish the flavor of, and can reduce phlegm, and ends phlegm, Dingchuan, regulating the qi flowing in the channels, flat liver-yang ".The pharmacological action of " Chinese medicinal fungi " bibliographical information gold ear is: 1, hypoglycemic, reducing blood lipid; 2, reduce the total fat of liver, liver cholesterol, improve hepatoprotective effects such as paddy Guang order peptide peroxidase activity; 3, immuno-potentiation; 4, promote hemopoietic function; 5, anti-aging effects; 6, anti-radiation function; 7, anti-inflammatory action; 8, the treatment heart, brain, vascular disease function.
For the peculiar resource of development and utilization gold this Chinese natural phant of ear, the existing scientific research institution of China has successively finished the pharmacological testing of golden fungus substituting stuff cultivation and Jin Er, prove that golden ear has kobadrin, antagonism tracheospasm, improves the abdominal cavity and gulp down the cytophilic effect of having a liking for function that gulps down, and can improve liver, spleen index, effectively reduce serum level of glucose, significantly improve indexs such as glucose tolerance and serum cholesterol, sweet three esters, glycosylated plasma protein, can significantly promote simultaneously hemolytic antibody to form, hemopoietic function improvement improves immunologic injury.
Yet, to understand according to the applicant, prior art never provides method how to extract useful component in the golden ear, has therefore restricted the development and use of the peculiar natural plant resource of this China.
Summary of the invention
The objective of the invention is to: provide a kind of main component that adopts the tremella filament to extract golden ear---the method for golden fungus polysaccharides, thereby lay the foundation for the business development utilization of golden ear.
In order to reach above purpose, method for extracting tremella mesenterica polysaccharide of the present invention may further comprise the steps:
A, put jar---golden ear is put into whizzer, and mycelium is taken out in centrifugation, washes the back with water and dries;
B, boiling---in mycelium, add boiling pure water;
C, hydrolysis---in cooking liquor, add highly basic to 0.3～0.8mol/L, make its hydrolysis;
D, centrifugal---separate hydrolyzed solution, get ultrafiltrated;
E, ultrafiltration---with 0.05～0.15 micron membrane microfiltration, use the membrane ultrafiltration of molecular weight 4500～5500 again;
F, concentrated---with alcohol ultrafiltration concentration liquid, make it precipitation;
G, crystallization---leach the concentrating and precipitating thing,, can obtain golden fungus polysaccharides with neutral spirits washing back oven dry.
The present invention has provided practicable method for extracting tremella mesenterica polysaccharide, by this method, can obtain main component---the golden fungus polysaccharides of golden ear simple and conveniently, thereby makes the peculiar natural phant of China---and the industrialized developing utilization of golden ear becomes possibility.
Embodiment
Below in conjunction with embodiment method of the present invention is described further.
Embodiment one
Present embodiment is fit to suitability for industrialized production, and extracting method is from the cultivation of tremella kind, and until final acquisition golden fungus polysaccharides, its concrete processing step comprises:
A ', cultivation
1), spawn culture---add water in potato, ratio is the 100g potato: 1000ml water, boil after-filtration; Sugaring and agar in filtrate, ratio are 1000ml filtrate: 20g sugar: 18g agar; Sterilize behind the packing test tube, the inclined-plane is put in cooling again; Normal temperature inserts the tremella kind, cultivate a week after, put refrigerator ripe one month;
2), the wheat bran bacterial classification---add water in wheat bran, ratio is 150g wheat bran: 100ml, stirs; Be divided in the postcooling of sterilizing in the triangular flask; Insert the bacterial classification in the test tube, put refrigerator ripe one month;
3), seed tank culture---with Semen Maydis powder, KH
2PO
4, MgSO
4, sugar, soya-bean oil mixes in the one-level jar with soil, ratio is Semen Maydis powder 2.5%: KH
2PO
40.3%, MgSO
41.5%, sugar 2%, soya-bean oil 0.65%, all the other are soil, after the sterilization wheat bran bacterial classification flame are inserted, and cultivate two days;
4), fermentor cultivation---with Semen Maydis powder, KH
2PO
4, MgSO
4, soybean cake powder, soya-bean oil mix in fermentor tank with soil, ratio is Semen Maydis powder 10%: KH
2PO
43%, MgSO
41.5%, soybean cake powder 0.2%, soya-bean oil 0.65%, all the other are soil, after the sterilization seeding tank bacterial classification are moved into 25 ℃ of fermentation culture;
A, put jar---cultured fermentor tank is put into whizzer, and mycelium is taken out in centrifugation, dries after washing 2 times with water;
B, boiling---in mycelium, add 10 times of pure water, boiled 4 hours, dry, repeat 4 times with whizzer;
C, hydrolysis---4 cooking liquors are merged, and adding NaOH is 0.5mol/L to concentration, hydrolysis 10 hours;
D, centrifugal---separate hydrolyzed solution, get super liquid;
E, ultrafiltration---with 0.1 micron membrane microfiltration, use the membrane ultrafiltration of molecular weight 5000 again;
F, concentrated---with 4 times of alcohol ultrafiltration concentration liquid, make it precipitation;
G, crystallization---leach the concentrating and precipitating thing,, obtain golden fungus polysaccharides with neutral spirits washing back oven dry;
H, packing---crystallization is pulverized evenly packing;
I, check
The processing step of present embodiment is from the most initial spawn culture, until final test package, and the process complete display.Experiment showed, the extraction that not only can realize golden fungus polysaccharides according to the method for present embodiment, and can suitability for industrialized production, the commercial exploitation of golden ear is become a reality.The golden fungus polysaccharides that extracts by the present embodiment method can be used as the main raw material of plurality kinds of health care food, medicine, and finished product all have the ideal result of treatment to multiple person in middle and old age's common diseases such as hyperlipidemia, diabetes.
Claims (6)
1. method for extracting tremella mesenterica polysaccharide is characterized in that may further comprise the steps:
A. put jar---golden ear is put into whizzer, and mycelium is taken out in centrifugation, washes the back with water and dries;
B, boiling---in mycelium, add boiling pure water;
C. hydrolysis---in cooking liquor, add highly basic to 0.3～0.8mol/L, make its hydrolysis;
D. centrifugal---separate hydrolyzed solution, get ultrafiltrated;
E. ultrafiltration---with 0.05～0.15 micron membrane microfiltration, use the membrane ultrafiltration of molecular weight 4500～5500 again;
F, concentrated---with alcohol ultrafiltration concentration liquid, make it precipitation;
G, crystallization---leach the concentrating and precipitating thing,, obtain golden fungus polysaccharides with neutral spirits washing back oven dry.
2. according to the described method for extracting tremella mesenterica polysaccharide of claim 1, it is characterized in that: also comprise spawn culture, promptly add water in potato, ratio is the 100g potato: 1000ml water, boil after-filtration; Sugaring and agar in filtrate, ratio are 1000ml filtrate: 20g sugar: 18g agar; Sterilize behind the packing test tube, the inclined-plane is put in cooling again; Normal temperature inserts the tremella kind, cultivate a week after, put refrigerator ripe one month.
3. according to the described method for extracting tremella mesenterica polysaccharide of claim 2, it is characterized in that also comprising the wheat bran bacterial classification, promptly add water in wheat bran, ratio is 150g wheat bran: 100ml, stirs; Be divided in the postcooling of sterilizing in the triangular flask; Insert the bacterial classification in the test tube, put refrigerator ripe one month.
4. according to the described method for extracting tremella mesenterica polysaccharide of claim 3, it is characterized in that: also comprise seed tank culture, be about to Semen Maydis powder, KH
2PO
4, MgSO
4, sugar, soya-bean oil mixes in the one-level jar with soil, part by weight is Semen Maydis powder 2.5%: KH
2PO
40.3%, MgSO
41.5%, sugar 2%, soya-bean oil 0.65%, all the other are soil, after the sterilization wheat bran bacterial classification flame are inserted, and cultivate two days.
5. according to the described method for extracting tremella mesenterica polysaccharide of claim 4, it is characterized in that also comprising fermentor cultivation, be about to Semen Maydis powder, KH
2PO
4, MgSO
4, soybean cake powder, soya-bean oil mix in fermentor tank with soil, part by weight is Semen Maydis powder 10%: KH
2PO
43%, MgSO
41.5%, soybean cake powder 0.2%, soya-bean oil 0.65%, all the other are soil, after the sterilization seeding tank bacterial classification are moved into 25 ℃ of fermentation culture.
6. according to the arbitrary described method for extracting tremella mesenterica polysaccharide of claim 1 to 5, it is characterized in that: in mycelium, add 10 times of pure water in the boiling step, boiled 4 hours, dry, repeat 4 times with whizzer; In hydrolysing step 4 cooking liquors are merged, adding NaOH is 0.5mol/L to concentration, hydrolysis 10 hours; In ultrafiltration step,, use the membrane ultrafiltration of molecular weight 5000 again with 0.1 micron membrane microfiltration; In enrichment step,, make it precipitation with 4 times of alcohol ultrafiltration concentration liquid; Final pulverizing, package test.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021126917A CN1151175C (en) | 2002-02-22 | 2002-02-22 | Method for extracting tremella mesenterica polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021126917A CN1151175C (en) | 2002-02-22 | 2002-02-22 | Method for extracting tremella mesenterica polysaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1367182A CN1367182A (en) | 2002-09-04 |
| CN1151175C true CN1151175C (en) | 2004-05-26 |
Family
ID=4742185
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021126917A Expired - Fee Related CN1151175C (en) | 2002-02-22 | 2002-02-22 | Method for extracting tremella mesenterica polysaccharide |
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Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225361B (en) * | 2007-01-18 | 2010-07-07 | 章克昌 | Golden fungus fermentation liquor or sesquiterpenes produced by fermentation of golden fungus liquid |
| CN102961297A (en) * | 2012-12-19 | 2013-03-13 | 李卫旗 | Novel moisturizing and ageing resisting skin care product containing natural tremella polysaccharide |
| CN103145865B (en) * | 2013-02-21 | 2015-12-02 | 北京绿科天成生物科技有限公司 | The preparation method of stereum hirsutum mycelium polysaccharide and application thereof |
| CN103408672B (en) * | 2013-07-15 | 2016-05-18 | 上海家化联合股份有限公司 | A kind of low-molecular-weight golden fungus polysaccharides and preparation method thereof |
| CN103555787A (en) * | 2013-10-16 | 2014-02-05 | 江南大学 | Preparation method of tremella aurantialba fermentation liquid polysaccharide with anti-oxidation activity |
| CN103519161A (en) * | 2013-11-01 | 2014-01-22 | 王颖 | Method for extracting golden fungus essence materials |
| CN103923222B (en) * | 2014-04-29 | 2016-04-13 | 中华全国供销合作总社昆明食用菌研究所 | A kind of low viscosity golden fungus polysaccharides extraction and separation method |
| CN104382956B (en) * | 2014-12-01 | 2017-10-13 | 北京绿科天成生物科技有限公司 | Golden ears or side handles of a utensil entity saponin extract and its production and use |
| CN106496343A (en) * | 2016-10-20 | 2017-03-15 | 广东真丽斯化妆品有限公司 | A kind of golden fungus polysaccharides extract and its application in cosmetics |
| CN107212333A (en) * | 2017-07-10 | 2017-09-29 | 张志才 | The fermentate of the golden solid state transformed Chinese yam of ear and its application |
| CN107383228A (en) * | 2017-08-18 | 2017-11-24 | 合肥丰洁生物科技有限公司 | A kind of method for extracting high purity of tremella polysaccharides |
| CN111690543B (en) * | 2020-06-30 | 2022-08-02 | 南京工业大学 | Tremella aurantialba and application thereof in preparation of tremella aurantialba fermented soybean milk |
-
2002
- 2002-02-22 CN CNB021126917A patent/CN1151175C/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN1367182A (en) | 2002-09-04 |
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