CN115109711B - Composite microbial inoculum, preparation method thereof and sewage treatment process - Google Patents
Composite microbial inoculum, preparation method thereof and sewage treatment process Download PDFInfo
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- 239000002131 composite material Substances 0.000 title claims abstract description 86
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- 238000000034 method Methods 0.000 title claims abstract description 26
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- 238000002360 preparation method Methods 0.000 title claims abstract description 10
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- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 8
- 229940069338 potassium sorbate Drugs 0.000 claims description 8
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds
- C02F2103/365—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds from petrochemical industry (e.g. refineries)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a composite microbial inoculum, a preparation method thereof and a sewage treatment process, and belongs to the field of petrochemical industry. The composite microbial inoculum comprises: the bacillus subtilis ZX2 and the Brucella ZY3 are preserved in the China general microbiological culture Collection center (CGMCC) with the preservation number of 21653; the Brucella ZY3 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.21654. The composite microbial inoculum can strengthen the original sewage treatment system by a biological method to improve the removal efficiency of CODcr and total nitrogen in the sewage, so that the composite microbial inoculum reaches the sewage discharge standard, and has the advantages of lower cost, short adaptation period and good compatibility.
Description
Technical Field
The invention relates to the field of petrochemical industry, in particular to a composite microbial inoculum, a preparation method thereof and a sewage treatment process.
Background
Petroleum contains more macromolecular organic matters such as colloid, asphaltene and the like, so that sewage (also called refining sewage) generated in the petroleum processing process has the characteristics of more toxic substances, serious emulsification, poor biodegradability, high load of organic pollutants and the like. Because of the above characteristics of the sewage, the sewage of the refinery is generally treated by the process flow of oil separation, air floatation and biochemistry, for example, the sewage treatment field of petrochemical division company in Liaoheng, china adopts the comprehensive treatment process of oil storage tank, inclined plate oil separation, secondary dissolved air floatation, primary hydrolysis acidification tank, CAST tank, secondary hydrolysis acidification tank, biological Aerated Filter (BAF), lanmeila sedimentation tank, detection tank and discharge.
After the sewage is treated by the method, the quality of the yielding water can basically reach the discharge standard, but the sewage treatment field still has the problems of unstable water inflow, leakage of an upstream device and the like, so that the sewage treatment field is frequently impacted by toxic and harmful sewage, and the sewage treatment field cannot quickly recover the sewage treatment function after being impacted. Once the sewage treatment field is subjected to the sudden impact, the sewage discharge is not up to standard within 1-2 weeks, and the whole sewage treatment field is in a paralyzed state when serious, which seriously affects normal production. Aiming at the situation, the biological microbial inoculum is added into a sewage treatment field impacted by high-toxicity and high-load sewage at present, and the specific flow is as follows: (1) Completely discharging the sewage and sludge of the biological section of the impacted sewage treatment plant; (2) Introducing impacted sewage and adding a composite microbial agent into a sewage treatment field to reestablish a new microbial ecological system. Among them, the bacterial agents are those sold by NoveXin or Biwofeng.
In carrying out the invention, the present inventors have found that there are at least the following problems in the prior art:
(1) The biological bacterial agent is generally exogenous bacteria, and if the exogenous bacteria and indigenous bacteria have substrate competition relationship, the addition of the exogenous bacteria is extremely easy to damage the original microbial ecological system of the sewage treatment plant.
(2) The operation flow of adding the microbial inoculum is complex.
(3) The microbial inoculum is expensive, resulting in high costs.
Disclosure of Invention
In view of the above, the invention provides a composite microbial inoculum, a preparation method thereof and a sewage treatment process, which can solve the technical problems.
Specifically, the method comprises the following technical scheme:
in one aspect, an embodiment of the present invention provides a composite microbial inoculant, where the composite microbial inoculant includes: bacillus subtilis ZX2 and brucella ZY3;
the bacillus subtilis ZX2 is preserved in the center of common microorganisms of China Committee for culture Collection of microorganisms, and the preservation number is CGMCC No.21653;
the Brucella ZY3 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.21654.
In some possible implementations, the mass ratio of the bacillus subtilis ZX2 to the brucella ZY3 is 2-3:1.
On the other hand, the embodiment of the invention also provides a preparation method of the composite microbial inoculum, which comprises the following steps: respectively culturing strains of bacillus subtilis ZX2 and brucella ZY3 in an LB culture medium for a set time to obtain bacillus subtilis ZX2 and brucella ZY3;
compounding the bacillus subtilis ZX2 and the brucella ZY3 to obtain the composite microbial inoculum;
the bacillus subtilis ZX2 is preserved in the center of common microorganisms of China Committee for culture Collection of microorganisms, and the preservation number is CGMCC No.21653;
the Brucella ZY3 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.21654.
In some possible implementations, the compounding the bacillus subtilis ZX2 and the brucella ZY3 to obtain the composite microbial agent includes:
according to the mass ratio of the bacillus subtilis ZX2 to the brucella ZY3, compounding a culture solution in which the bacillus subtilis ZX2 and the brucella ZY3 are cultured to obtain a compound bacterial solution with the compound bacterial agent;
sequentially concentrating the compound bacterial liquid, and adding potassium sorbate into the compound bacterial liquid to obtain concentrated bacterial liquid;
uniformly mixing the sterilized carrier and the concentrated bacterial liquid, drying to a set water content, adding a deoxidizer into the mixed system, and uniformly mixing to obtain dry powder of the composite bacterial agent;
and (5) sealing and preserving the dry powder of the composite microbial inoculum.
In some possible implementations, after the pH adjustment is sequentially performed on the compound bacterial solution, the pH of the compound bacterial solution is adjusted to 3.
In some possible implementations, the mass concentration of the potassium sorbate in the concentrated bacterial liquid is 2% -3%;
in the dry powder of the composite microbial inoculum, the mass concentration of the deoxidizer is 2-3%.
In some possible implementations, the carrier is selected from wheat bran, diatomaceous earth, or talc.
In some possible implementations, when the carrier is selected from wheat bran or diatomaceous earth, the mass ratio of the carrier to the concentrated bacterial liquid is 1:2-3;
when the carrier is selected from talcum powder, the mass ratio of the carrier to the concentrated bacterial liquid is 2-3:1.
In still another aspect, an embodiment of the present invention further provides a sewage treatment process, including: directly adding a composite microbial inoculum into a sewage treatment field impacted by sewage to treat the sewage;
wherein the composite microbial inoculum is shown in the first aspect related to the embodiment of the invention.
In some possible implementations, the composite microbial inoculum is added in an amount of 1g-3g per 1000mL of sewage to be treated.
The technical scheme provided by the embodiment of the invention has the beneficial effects that at least:
the composite microbial inoculum provided by the embodiment of the invention uses the bacillus subtilis ZX2 and the brucella ZY3, so that a good degradation effect on sewage refining and chemical wastewater can be obtained, and organic matters and total nitrogen which are difficult to degrade in the sewage refining and chemical wastewater can be effectively removed. The composite microbial inoculum can strengthen the original sewage treatment system by a biological method to improve the removal efficiency of CODcr and total nitrogen in the sewage, so that the sewage reaches the sewage discharge standard.
The bacillus subtilis ZX2 and the brucella ZY3 are separated from sewage in a sewage treatment field, and have at least the following advantages:
(1) The bacillus subtilis ZX2 and the brucella ZY3 belong to indigenous bacteria of a sewage treatment plant, have the advantages of short strain adaptation period, good compatibility, strong specificity and the like, and are beneficial to increasing the treatment efficiency of the sewage treatment plant.
(2) The microbial inoculum is simple in adding operation flow, and after the sewage treatment field is impacted by high-toxicity and high-load sewage, the composite microbial inoculum is directly added into the sewage treatment field impacted by the sewage according to the proportion, so that the complex operation process of discharging sewage and sludge in the sewage treatment field is avoided.
(3) The cost of the composite microbial inoculum prepared by the invention is lower.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram showing the influence of the compounding ratio of bacillus subtilis ZX2 and Brucella ZY3 on the biodegradability of sewage and the removal rate of COD;
FIG. 2 is a schematic diagram showing the effect of different carrier types on the bacterial load;
FIG. 3 is a schematic diagram showing the degradation effect of different carrier types on COD of organic pollutants;
FIG. 4 is a schematic diagram showing the degradation effect of the addition amount of the compound microbial inoculum on the COD of the organic pollutant;
FIG. 5 is a graph showing the COD content of the CAST reactor at the impact stage of high-toxicity and high-load organic sewage;
FIG. 6 is a graph of total nitrogen content for the CAST reactor during the high-toxicity high-load organic wastewater impact stage;
FIG. 7 is a graph showing COD content of the CAST reactor at the impact stage of high-toxicity high-load organic sewage and hydraulic load;
FIG. 8 is a total nitrogen content line graph of the CAST reactor during the high-toxicity high-load organic wastewater and hydraulic load impact phase.
Detailed Description
In order to make the technical scheme and advantages of the present invention more apparent, embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.
At present, biological bacteria are generally added into a sewage treatment field subjected to high-toxicity and high-load sewage impact to treat sewage in the sewage treatment field, and the specific flow is as follows: (1) Completely discharging the sewage and sludge of the biological section of the impacted sewage treatment plant; (2) Introducing impacted sewage and adding a compound microbial agent into a sewage treatment field to reestablish a new microbial ecological system. Among them, the biological bacterial agents are used, for example, those sold by Novelis corporation or Bivolfeng corporation.
However, the above prior art has at least the following problems:
(1) The biological bacterial agent is generally exogenous bacteria, and if the exogenous bacteria and indigenous bacteria have substrate competition relationship, the addition of the exogenous bacteria is extremely easy to damage the original microbial ecological system of the sewage treatment plant.
(2) The operation flow of adding the microbial inoculum is complex.
(3) The microbial inoculum is expensive, resulting in high costs. The price of the related microbial inoculum sold by NoveXin or Biwofeng is about 70-80 ten thousand RMB per ton, the dosage of the microbial inoculum is generally more than 1% (mass ratio), and the microbial inoculum is required to be periodically added in order to prevent degradation of the microbial inoculum, so that the microbial inoculum is easy to generate dependence.
The embodiment of the invention provides a composite microbial inoculant, which comprises the following components: bacillus subtilis ZX2 and brucella ZY3.
The bacillus subtilis ZX2 is preserved in the China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.21653 and the preservation date of 2021, 01 and 18 days, and the classification name of the bacillus subtilis ZX2 is bacillus subtilis Bacillus subtilis; wherein, the China general microbiological culture Collection center is located at the national academy of sciences of China microbiological culture Collection center, national institute No. 1, 3, north Chen West Lu, korea, beijing city.
Brucella ZY3 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.21654 and the preservation date of 2021, 01 and 18 days, and the classification name of Brucella ZY3 is Brucella sp; wherein, the general microbiological center of China Committee for microbiological culture Collection is located at microbiological institute of China academy of sciences of North Chenxi Lu No. 1 and No. 3 in the Korean area of Beijing city.
In some possible implementations, the mass ratio of bacillus subtilis ZX2 to brucella ZY3 is 2-3:1, e.g., the mass ratio of bacillus subtilis ZX2 to brucella ZY3 is 3:1.
In the embodiment of the invention, bacillus subtilis ZX2 and brucella ZY3 are separated from the sewage in the sewage treatment plant.
In current sewage treatment systems, such as sewage treatment plants, there are a large number of microorganisms, especially bacteria, which degrade organic matter, which are the main microbial flora in the sewage. In order to improve the degradation effect on the sewage, the embodiment of the invention adopts a common microorganism separation method in the field to separate bacteria from the sewage in the sewage treatment field. For example, distilled water is used for diluting the sewage, the sewage is diluted to a multiple of 10 (for example, the dilution multiple of the sewage is 100 times, 1000 times, etc.), then the diluted sewage is coated on a nutrient agar solid culture medium, after culturing for 24-48 hours at 37 ℃, different bacteria grow on the solid culture medium in different colony forms, and the different colonies are separated and cultured separately to examine the degradability of each colony on organic matters and the degradation rate thereof, so that the desired degradation strain can be screened.
Through the experiment, the bacillus subtilis ZX2 and the brucella ZY3 are screened out and used as expected sewage degradation strains.
The composite microbial inoculum provided by the embodiment of the invention uses the bacillus subtilis ZX2 and the brucella ZY3, so that a good degradation effect on sewage refining and chemical wastewater can be obtained, and organic matters and total nitrogen which are difficult to degrade in the sewage refining and chemical wastewater can be effectively removed. The composite microbial inoculum can strengthen the original sewage treatment system by a biological method to improve the removal efficiency of CODcr and total nitrogen in the sewage, so that the sewage reaches the sewage discharge standard.
Since bacillus subtilis ZX2 and brucella ZY3 are separated from sewage in the sewage treatment plant to be treated, the method has at least the following advantages:
(1) The bacillus subtilis ZX2 and the brucella ZY3 belong to indigenous bacteria of a sewage treatment plant, have the advantages of short strain adaptation period, good compatibility, strong specificity and the like, and are beneficial to increasing the treatment efficiency of the sewage treatment plant.
(2) The microbial inoculum is simple in adding operation flow, and after the sewage treatment field is impacted by high-toxicity and high-load sewage, the composite microbial inoculum is directly added into the sewage treatment field impacted by the sewage according to the proportion, so that the complex operation process of discharging sewage and sludge in the sewage treatment field is avoided.
(3) The cost of the composite microbial inoculum prepared by the invention is lower.
On the other hand, the embodiment of the invention also provides a preparation method of the composite microbial inoculum, which comprises the following steps: culturing the bacillus subtilis ZX2 and the brucella ZY3 in an LB culture medium for a set time to obtain the bacillus subtilis ZX2 and the brucella ZY3. Wherein, the bacillus subtilis ZX2 and the brucella ZY3 strains are separated from sewage in a sewage treatment field to be treated.
According to the mass ratio of the bacillus subtilis ZX2 to the brucella ZY3, the bacillus subtilis ZX2 and the brucella ZY3 are compounded to obtain the composite microbial inoculum.
Among them, LB medium is common in the art, for example, LB liquid medium L1010 sold by Beijing Soy Bao technology Co., ltd or LB liquid medium PM0010-50 sold by Beijing blue Boston biotechnology Co., ltd are suitable for the present invention.
In some possible implementation manners, in the embodiment of the present invention, according to a mass ratio of bacillus subtilis ZX2 to brucella ZY3, the bacillus subtilis ZX2 and the brucella ZY3 are compounded to obtain a composite microbial inoculum, which includes:
according to the mass ratio of bacillus subtilis ZX2 to brucella ZY3, the culture solution in which the bacillus subtilis ZX2 and the brucella ZY3 are cultured is compounded, and the compound bacterial solution with the compound bacterial agent is obtained.
And (3) concentrating the compound bacterial liquid in sequence, and adding potassium sorbate into the compound bacterial liquid after pH adjustment to obtain concentrated bacterial liquid.
And uniformly mixing the sterilized carrier and the concentrated bacterial liquid, drying to a set water content, adding a deoxidizer into the mixed system, and uniformly mixing to obtain the dry powder of the composite bacterial agent.
And (5) sealing and preserving the dry powder of the composite microbial inoculum.
The method can prepare the dry powder composite microbial inoculum, so that the activity of bacterial cells is reduced and even dormant, and the method has the advantages of long storage time, low storage and transportation cost and the like. Therefore, when the composite microbial inoculum is applied, the sewage treatment field impacted by polluted water can be restored to the normal treatment level in a short time by only directly adding the composite microbial inoculum prepared by the invention into the sewage of the sewage treatment field.
In some possible implementations, the multiple of concentration of the compounded bacterial liquid is 0.5 times to ensure that the bacterial agent activity is not compromised.
After the compound bacterial liquid is concentrated, the pH value of the compound bacterial liquid is adjusted to 3 so as to provide an adaptive living environment.
And (3) concentrating the compound bacterial liquid in sequence, and adding potassium sorbate into the compound bacterial liquid after pH adjustment so as to achieve the aim of corrosion prevention. Wherein, in the concentrated bacterial liquid, the mass concentration of potassium sorbate is 2% -3%.
And uniformly mixing the sterilized carrier and the concentrated bacterial liquid, drying to a set water content, adding a deoxidizer into the mixed system, and uniformly mixing to obtain the dry powder of the composite bacterial agent.
Illustratively, the carrier and concentrated bacterial solution are placed in a constant temperature incubator at 35 ℃ and dried to a moisture content of 6wt% to 10wt%.
The deoxidizer can be an iron powder deoxidizer; the mass concentration of the deoxidizer in the dry powder of the composite microbial inoculum is 2-3%.
In some possible implementations, the carrier is selected from wheat bran, diatomaceous earth, or talc.
When diatomaceous earth or wheat bran having a relatively large water holding capacity is used as a carrier, the bacterial absorption amount of the composite bacterial agent increases with the time. The diatomite and the talcum powder belong to inorganic carriers, the organic load of raw water is not increased, and the degradation of organic pollutants by the composite microbial inoculum taking the talcum powder or the diatomite as the carrier is shown as a trend of firstly reducing and then unchanged. Based on the above, in the embodiments of the present invention, it is preferable to use diatomaceous earth, which is an inorganic material that absorbs water easily, as a carrier.
In particular, when the carrier is selected from wheat bran or diatomaceous earth, the mass ratio of the carrier to the concentrated bacterial liquid is 1:2-3, for example 1:2;
when the carrier is selected from talcum powder, the mass ratio of the carrier to the concentrated bacterial liquid is 2-3:1, for example, 2:1.
According to the embodiment of the invention, bacteria are adsorbed based on the carrier with the mass ratio, and after subsequent treatments such as drying, the activity of bacterial microorganism cells can be reduced and even dormant, and the method has the advantages of long storage time and low storage and transportation cost.
On the other hand, the embodiment of the invention also provides a sewage treatment process, which comprises the following steps: and directly adding a composite microbial inoculum into a sewage treatment field impacted by sewage to treat the sewage.
Wherein, the composite microbial agent is as described in the first aspect of the embodiment of the present invention, that is, the composite microbial agent includes: the mass ratio of the bacillus subtilis ZX2 to the brucella ZY3 is 2-3:1; and, bacillus subtilis ZX2 and brucella ZY3 are separated from sewage in a sewage treatment field to be treated.
In order to obtain good degradation effect and avoid bacterial waste, the adding amount of the composite bacterial agent is 1g-3g in every 1000mL sewage to be treated.
When the sewage treatment field is impacted by toxic and harmful sewage and is impacted by high hydraulic load, the impact on the sewage treatment field can be effectively buffered by utilizing the composite microbial inoculum provided by the embodiment of the invention.
Through experimental tests, when sewage COD is fed into the aerobic reactor cr 300-800mg/L, high toxicity, 15-60 ℃ of treatment temperature, 6-9 of pH value, 2-5mg/L of Dissolved Oxygen (DO) and 60-70h of Hydraulic Retention Time (HRT), 1-3 per mill (mass ratio) of composite microbial agent and 5-10mg/L of organic nutritional agent required by microorganism growth are added into an aerobic reactor, so that the impact resistance of the aerobic reactor can be improved.
Wherein, the organic nutrient is a substance required by the growth of microorganisms commonly used by researchers in the field, such as organic matters like peptone, yeast powder and the like.
The invention will be further described by means of specific examples:
example 1
In this example 1, the influence of the compounding ratio of bacillus subtilis ZX2 and brucella ZY3 on sewage treatment was tested:
the compound sewage related in the embodiment 1 is the effluent of the periodic cycle activated sludge process (Cyclic Activated Sludge Technology, CAST) and the secondary dissolved air flotation influent, and the volume ratio is 9:1.
As shown in FIG. 1, when the compounding ratio of the bacillus subtilis ZX2 to the brucella ZY3 is 3:1, the treatment effect on the compounded sewage is best, the B/C ratio is more than 0.42, the biodegradability is good, and the COD removal rate is higher than 60%. Meanwhile, the test also shows that when the composite ratio of the bacillus subtilis ZX2 and the brucella ZY3 is gradually reduced from 3:1 to 1:3, the B/C and COD removal rate of the sewage is gradually reduced, and the treatment effect is poor, probably because of the substrate competition relationship between the bacillus subtilis ZX2 and the brucella ZY3.
Therefore, in the actual working condition of sewage treatment, the mass ratio of the bacillus subtilis ZX2 to the brucella ZY3 is limited to 2-3:1.
Example 2
The embodiment 2 provides a composite microbial agent, which is prepared by the following preparation method:
(1) Sterilizing the pretreated clean carrier at 121deg.C for 15min. Wherein the carrier comprises wheat bran, diatomite and talcum powder, and the preparation of the composite microbial inoculum is respectively carried out.
(2) Culturing bacillus subtilis ZX2 and brucella ZY3 in an LB culture medium for 24 hours respectively, and then obtaining the bacillus subtilis according to the mass ratio: the ratio of brucella=3:1, and the culture solution in which the bacillus subtilis ZX2 and the brucella ZY3 are cultured is compounded to obtain a compound bacterial solution with a compound bacterial agent. Concentrating the compound bacterial liquid by 0.5 times in sequence, adjusting the pH value to 3, and adding 2wt% of potassium sorbate into the compound bacterial liquid to obtain concentrated bacterial liquid.
(3) According to wheat bran or kieselguhr: mixing the carrier and the concentrated bacterial liquid uniformly according to the mass ratio of concentrated solution=1:2 and talcum powder to concentrated solution=2:1; drying in a constant temperature incubator at 35deg.C until the water content is 8wt%, adding 2wt% of iron powder deoxidizer, mixing, and sealing for storage.
As shown in FIG. 2, the bacteria-absorbing amount of the composite microbial inoculum using diatomite and wheat bran as carriers increases with the time, and after one week, the bacteria-containing amounts of the two carriers are basically the same, and the higher bacteria-containing amount is maintained.
The microbial inoculum with talcum powder as a carrier has relatively less bacteria absorption, and the analysis reasons may be that the talcum powder has less water holding capacity, and the talcum powder is dehydrated and dried in the preservation process of the microbial inoculum, so that the growth and propagation of bacteria are not facilitated.
The diatomite and the wheat bran have larger bacteria absorption amount, but in comparison with the diatomite and the wheat bran, the wheat bran belongs to an organic carrier, and the organic load of raw water can be increased in the process of adding the microbial inoculum, so that the COD of the effluent is caused cr And (3) increasing. Therefore, this example 2 further examined the degradation effect of three carriers on the COD of the compounded sewage to determine the optimal carrier.
As shown in FIG. 3, the wheat bran as carrier of the composite microbial agent has the worst effect on degrading organic pollutants, and the microorganisms continuously act on the compound sewage for 96 hours to obtain COD cr Does not show good degradation effects. The reason for this is probably that wheat bran itself is an organic carrier, and microorganisms degrade macromolecular organic matters in the wheat bran into small molecular pollutants and dissolve the small molecular pollutants in water, so that the content of the organic pollutants in the compound sewage is increased, and the quality of the effluent is deteriorated.
The degradation of organic pollutants by the composite microbial inoculum taking talcum powder and diatomite as carriers is shown as a trend of firstly reducing and then keeping unchanged, and especially the COD of the compound sewage is compounded at 12h by taking diatomite as the composite microbial inoculum of the carriers cr The removal rate was 44.76% only at 60.87mg/L, and the final removal rate at 96 hours was 49.75%. Therefore, the implementation of the invention is feasible by preferably using the diatomite which is an easily water-absorbing inorganic porous material as the optimal carrier of the composite microbial inoculum.
Example 3
In this example 3, the effect of the addition amount of the composite microbial inoculum on the degradation of the organic pollutants was tested, and the test result is shown in fig. 4, and the COD of the effluent of the compound sewage is compounded with the addition amount of the composite microbial inoculum as shown in the figure 4 cr Gradually rise because: when a small amount of the compound microbial inoculum is added, the content of nutrient substances in the compound microbial inoculum is small and insufficient to cause interference to the compound sewage; along with the increasing of the adding amount of the compound bacterial agent, the influence of the nutrient substances in the compound bacterial agent on the compound sewage is gradually revealed, so that the content of organic pollutants in the compound sewage can be accumulated, and the COD of the effluent is further influenced.Through testing, the expected dosage of the composite microbial inoculum is 1-3 per mill, that is, the dosage of the composite microbial inoculum is 1-3 g in each 1000mL of compound sewage.
Example 4
In this example 4, a field experiment study was performed on a CAST reactor based on the composite microbial inoculum provided in the example of the invention, and the specific experiment is as follows:
experiment 1
When the CAST reactor is impacted by water inlet with high toxicity and high organic load, whether the impacted reactor can recover the function in a short time is tested by adding a certain amount of composite microbial inoculum and nutrient. Therefore, in order to better embody the impact effect, the on-site CAST water inlet (primary hydrolysis acidification water outlet) is changed into mixed sewage of crude oil pretreatment water and secondary dissolved air flotation water (the volume ratio of the crude oil pretreatment water to the secondary dissolved air flotation water is 1:6, the COD of the crude oil pretreatment water is 1500-2000mg/L, the organic load and toxicity of the mixed sewage are far higher than those of the primary hydrolysis acidification water outlet), the adding amount of the composite microbial inoculum is determined to be 1 per mill, the adding amount of the organic nutrient is 7mg/L, and the other parameters are consistent with the on-site working conditions.
COD cr The degradation effect results are shown in figure 5, and the COD of the effluent of the No. 1 reactor without the microbial inoculum is shown cr The sludge sedimentation ratio is reduced from 35% to 20% at the highest of 106.80mg/L, which indicates that the CAST reactor is decomposed and deactivated under the continuous impact of high-toxicity and high-load sewage.
Compared with the No. 1 reactor, the No.2 reactor has COD of water discharged within 24 hours after the microbial inoculum is added cr A great decrease trend appears, and the COD of the reactor effluent is in 8 days later cr The sludge sedimentation ratio is always maintained between 40 and 60mg/L and is stabilized between 30 and 35 percent, which indicates that the running condition of the activated sludge is good.
When the CAST reactor runs to the 6 th day, effluent COD of the 1# reactor and the 2# reactor cr Similarly, the aerobic microorganisms in the reactor cannot fully exert the function of degrading organic pollutants, so that the effluent CODcr of the No.2 reactor has poor effect.
It can be seen that when the reactor without the composite microbial agent and the nutritional agent is continuously impacted by a toxic and organic load with higher strength, the recovery period of the reactor is prolonged and even disappears, and the reactor with the composite microbial agent and the nutritional agent has stronger impact load resistance.
The result of the total nitrogen degradation effect is shown in figure 6, and the result shows that the total nitrogen concentration of the inlet water of the No. 1 reactor and the No.2 reactor is more stable and is 20-30mg/L. And when the operation of the reactor is finished in the third day, the composite microbial inoculum and the nutritional agent are added into the No.2 reactor, and the No. 1 reactor maintains the original operation mode.
As shown in fig. 6, after the composite microbial inoculum and the nutrient are added, the total nitrogen concentration of the effluent of the # 1 reactor and the effluent of the # 2 reactor have the same trend, but the total nitrogen concentration of the effluent of the # 2 reactor is always lower than that of the # 1 reactor. This shows that after the toxicity and organic load of the influent sewage are improved, the treatment effect of the reactor can be effectively improved by adding the composite microbial inoculum, so that the sewage is discharged after reaching the standard.
Experiment 2
The purpose of experiment 2 was to examine whether the CAST reactor could quickly recover its processing function by adding a complex microbial agent and a nutrient pole when the CAST reactor was subjected to both high-toxicity high-organic load and hydraulic load.
COD cr The degradation results are shown in fig. 7, and the CAST reactor was operated for a total of 16 days, and all the operation conditions of the 1# reactor and the 2# reactor were the same for the first three days, in order to eliminate the difference between the two reactors themselves. And when the operation of the reactor is finished in the third day, the working condition is changed, the nutrient and the composite microbial inoculum are added into the No.2 reactor, the adding amount of the composite microbial inoculum is determined to be 2 per mill, and the adding amount of the organic nutrient is 8mg/L. The 1# reactor is blank.
In a week after the addition of the microbial inoculum and the nutrient, the effluent COD of the No.2 reactor cr Significantly lower than the # 1 reactor, about 10mg/L lower. This is because the composite microbial agent itself contains a large amount of highly efficient bacteria that degrade organic pollutants. After the composite microbial inoculum is added into the No.2 reactor, organic pollution is degraded in the reactorThe number of the high-efficiency bacteria of the substances is increased rapidly, and the number of the high-efficiency bacteria for degrading the organic pollutants in the reactor is reduced continuously because the reactor 1 is impacted by high-toxicity high-load sewage and high-hydraulic-load sewage continuously, so that the effluent water quality of the reactor 2 is better than that of the reactor 1. However, after one week, COD was obtained from the effluent of the two reactors cr Nearly identical. This indicates that the CAST reactor has a self-recovery function when it is impacted by a non-lethal load.
The result of the total nitrogen degradation effect is shown in figure 8, and in the experimental process, the total nitrogen fed into the CAST reactor has larger fluctuation, and the fluctuation range is 17.21-31.62mg/L. The total nitrogen of the effluent water of the No.2 reactor is reduced from 15.05mg/L of the inlet water to 7.79mg/L at the first day after the microbial inoculum and the nutrient are added, and the total nitrogen removal rate is 48.24%. However, the total nitrogen value of the effluent of the # 1 reactor and the # 2 reactor is kept the same in the two weeks after and the next day of the addition of the microbial inoculum, because the increase in the hydraulic load of the CAST reactor and the increase in the content of toxic substances in the influent water inhibit part of the functions of denitrifying bacteria in the microbial inoculum, and cannot exert the denitrification function thereof. Therefore, when the water inflow is increased by 1.5 times, toxic substances in the CAST reactor have become a major factor for inhibiting the growth and reproduction of denitrifying bacteria.
The foregoing description is only for the convenience of those skilled in the art to understand the technical solution of the present invention, and is not intended to limit the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A composite microbial inoculant, characterized in that the composite microbial inoculant comprises: bacillus subtilis ZX2 and brucella ZY3;
the bacillus subtilis ZX2 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC No.21653, and the classification of the bacillus subtilis ZX2 is named as bacillus subtilis Bacillus subtilis;
the Brucella ZY3 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC No.21654, and the classification of the Brucella ZY3 is named Brucella sp;
the mass ratio of the bacillus subtilis ZX2 to the brucella ZY3 is 2-3:1.
2. The preparation method of the composite microbial inoculum is characterized by comprising the following steps of: culturing bacillus subtilis ZX2 and brucella ZY3 strains in an LB culture medium for a set time to obtain bacillus subtilis ZX2 and brucella ZY3;
compounding the bacillus subtilis ZX2 and the brucella ZY3 to obtain the composite microbial inoculum;
the bacillus subtilis ZX2 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC No.21653, and the classification of the bacillus subtilis ZX2 is named as bacillus subtilis Bacillus subtilis;
the Brucella ZY3 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC No.21654, and the classification of the Brucella ZY3 is named Brucella sp;
the mass ratio of the bacillus subtilis ZX2 to the brucella ZY3 is 2-3:1;
wherein, the compounding of the bacillus subtilis ZX2 and the brucella ZY3 to obtain the composite microbial inoculum comprises the following steps:
according to the mass ratio of the bacillus subtilis ZX2 to the brucella ZY3, compounding a culture solution in which the bacillus subtilis ZX2 and the brucella ZY3 are cultured to obtain a compound bacterial solution with the compound bacterial agent;
sequentially concentrating the compound bacterial liquid, and adding potassium sorbate into the compound bacterial liquid to obtain concentrated bacterial liquid;
uniformly mixing the sterilized carrier and the concentrated bacterial liquid, drying to a set water content, adding a deoxidizer into the mixed system, and uniformly mixing to obtain dry powder of the composite bacterial agent;
sealing and preserving the dry powder of the composite microbial inoculum;
the carrier is selected from diatomite, and the mass ratio of the carrier to the concentrated bacterial liquid is 1:2-3.
3. The method for producing a composite microbial inoculum according to claim 2, wherein the pH of the composite microbial inoculum is adjusted to 3 after the pH adjustment is sequentially performed on the composite microbial inoculum.
4. The method for preparing the composite microbial inoculum according to claim 2, wherein the mass concentration of potassium sorbate in the concentrated microbial inoculum is 2% -3%;
in the dry powder of the composite microbial inoculum, the mass concentration of the deoxidizer is 2-3%.
5. A wastewater treatment process, characterized in that the wastewater treatment process comprises: directly adding a composite microbial inoculum into a sewage treatment field impacted by sewage to treat the sewage;
wherein the composite microbial inoculum is shown in claim 1.
6. The sewage treatment process according to claim 5, wherein the amount of the composite microbial inoculum added is 1g-3g per 1000mL of sewage to be treated.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2002125657A (en) * | 2000-10-20 | 2002-05-08 | Mitsui Eng & Shipbuild Co Ltd | New microorganism belonging to bacillus subtilis |
| WO2007073656A1 (en) * | 2005-12-27 | 2007-07-05 | Shenyang Institute Of Applied Ecology, Chinese Academy Of Sciences | A microbial preparation for treating thick oil sewage and method for preparing the same |
| CN102839124A (en) * | 2011-06-23 | 2012-12-26 | 中国石油天然气集团公司 | Separation screening method for extreme environment crude oil degradation function bacteria |
| CN110938567A (en) * | 2019-12-10 | 2020-03-31 | 中国农业科学院植物保护研究所 | Bacillus subtilis, microbial agent and application thereof |
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| US10179744B2 (en) * | 2017-01-12 | 2019-01-15 | Cisbay Global Inc. | Method for water remediation and desalination via selectively breeding and cultivating generations of microbes |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2002125657A (en) * | 2000-10-20 | 2002-05-08 | Mitsui Eng & Shipbuild Co Ltd | New microorganism belonging to bacillus subtilis |
| WO2007073656A1 (en) * | 2005-12-27 | 2007-07-05 | Shenyang Institute Of Applied Ecology, Chinese Academy Of Sciences | A microbial preparation for treating thick oil sewage and method for preparing the same |
| CN102839124A (en) * | 2011-06-23 | 2012-12-26 | 中国石油天然气集团公司 | Separation screening method for extreme environment crude oil degradation function bacteria |
| CN110938567A (en) * | 2019-12-10 | 2020-03-31 | 中国农业科学院植物保护研究所 | Bacillus subtilis, microbial agent and application thereof |
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