CN115104615A - Cell division promoting agent and preparation method thereof - Google Patents

Abstract

The invention provides a cell division promoter and a preparation method thereof. The invention takes the benzylaminopurine and the gibberellic acid with a specific ratio as active ingredients, remarkably improves the grape fruit setting rate, improves the grape yield by more than 10 percent, and simultaneously adopts a specific solvent to increase the stability of the prepared cell division promoting agent and effectively avoids the generation of precipitates in a system. In addition, the special citric acid is added, so that the stability of the gibberellic acid after spraying can be enhanced, and the gibberellic acid can play a long-acting role.

Description

Cell division promoting agent and preparation method thereof

Technical Field

The invention relates to the technical field of plant growth regulators, in particular to an IPC (International patent application for Industrial science) No. A01N59/00, and more particularly relates to a cell division promoting agent and a preparation method thereof.

Background

With the development and progress of society, more and more plant growth regulators appear in the agricultural field, the production amount and the dosage of the plant growth regulators are increased year by year, and although a single-component plant growth regulator has a good regulating effect on certain crops, the single-component plant growth regulator has a large limitation, the crops have strong selectivity, the effect is not ideal, and even potential hazard risks exist on certain crops. The compounding of the plant growth regulator not only can enlarge the regulation range and enhance the pesticide effect and selectivity, but also can reduce the dosage of the plant growth regulator, relatively reduce the production cost, avoid the pesticide damage and ensure the safety of crops.

Benzylaminopurine is the first artificially synthesized cytokinin, and has the effects of inhibiting the decomposition of chlorophyll, protein and nucleic acid in plant leaves, and effectively protecting green and preventing aging; can transport auxin, amino acid, inorganic salt and the like to a treatment part, can also induce bud differentiation, promote lateral bud growth, promote cell division and other effects, and is widely applied to various stages of crops such as agriculture, fruit trees and the like. Gibberellic acid has good broad spectrum, can promote the growth and the maturity of crops, can improve the quality of products, can reduce the shedding of flowers, buds, bolls and fruits, and can improve the fruit bearing rate or form seedless fruits.

However, the ratio of the effective components of the plant growth regulator has a key influence on the action of the medicament, the improper ratio not only cannot promote the growth of crops, but also can negatively influence the crops, and in addition, when different active components are mixed, the system can be unstable, so that the use effect of the plant growth regulator is influenced.

Chinese patent CN101836646A discloses a plant growth regulator containing gibberellic acid and benayl aminopurine, which can promote the absorption of crops, increase the chlorophyll content of plants, and assist the growth of crops. Chinese patent CN104692945A discloses a plant growth regulator containing foliar fertilizer, which has better effects of promoting growth and increasing yield for cucumber, orange, red date, etc. The active ingredients of these two patents are mainly gibberellic acid and benzylaminopurine, but the stability test of the prepared product is not carried out, and precipitation may be generated, thereby affecting the subsequent efficacy.

Disclosure of Invention

In view of the problems in the prior art, the present invention provides, in one aspect, a cell division promoting agent comprising an active ingredient and a solvent, wherein the active ingredient comprises benzylaminopurine and gibberellic acid.

In some preferred embodiments, 2-6 parts of active ingredient, solvent, make up to 100 parts by weight.

In some preferred embodiments, the weight ratio of benzylaminopurine to gibberellic acid is (0.5-1.5): 1.

preferably, the weight ratio of benzylaminopurine to gibberellic acid is 1: 1.

the applicant unexpectedly discovers that after 7500 times of diluent of the cell division promoting agent prepared in the application is used, the grape fruit setting rate can be remarkably improved, the grape yield is improved by more than 10%, the grape leaf surface possibly has concave and convex points with higher roughness, other proportions or other cell division agents are used, the concave and convex points on the grape leaf surface cause adsorption obstruction to the cell division agent, and the purine skeleton and the gibberellin skeleton molecular chain segments with specific proportions are selected in the cell division promoting agent prepared in the application, so that stable and dense hydrogen bond acting force can be formed, and after spraying, the concave and convex points on the grape leaf surface can be increased to be kinked, so that the effect is exerted.

In some preferred embodiments, the solvent comprises one or more of an organic alcohol, an amide solvent, and water.

Preferably, the solvent comprises organic alcohol, an amide solvent and water, and the weight ratio of the organic alcohol to the amide solvent is (5-7): 1: (2-4).

More preferably, the solvent comprises organic alcohol, amide solvent and water, and the weight ratio of the organic alcohol to the amide solvent is 6.4: 1: 2.6.

in some preferred embodiments, the organic alcohol is a hydroxyl terminated alcohol.

In some preferred embodiments, the hydroxyl terminated alcohol comprises ethanol.

In some preferred embodiments, the amide-based solvent has a viscosity of 0.8 to 1mpa.s at 25 ℃.

Preferably, the amide solvent has a viscosity of 0.92mpa.s at 25 ℃.

With the use of benzylaminopurine and gibberellic acid in the specific proportions indicated in this application, the fruit set rate of grapes in particular can be increased, but this leads to instability of the obtained cell division promoting agent, which precipitates over time, and especially increases the instability of the cell division promoting agent when the solvent comprises water, the applicant has surprisingly found that when the solvent comprises organic alcohol, amide solvent and water, and the weight ratio thereof is (5-7): 1: (2-4), especially when the organic alcohol is hydroxyl-terminated alcohol, and the viscosity of the amide solvent is 0.8-1mpa.s at 25 ℃, the stability of the obtained cell splitting agent is better, and no precipitation occurs after 48h, the applicant considers that the possible reason is that in the hydroxyl-terminated alcohol and the amide solvent with the specific proportion, the dissolution difference of the benzylaminopurine and the gibberellic acid in each component of the solvent is different, so that when the benzylaminopurine is expected to permeate the hydroxyl-terminated alcohol, the gibberellic acid is driven to permeate into the alcohol, especially the ethanol through intermolecular hydrogen bonds, and when the effect between ethanol molecules is increased, for example, the ethanol molecules are linked to the close amide solvent with the viscosity of 0.8-1mpa.s, especially the dimethylacetamide or the water timely participates in intermolecular van der waals force, so as to promote the generation of a stable system.

In some preferred embodiments, the cell-division promoting agent further comprises an organic acid.

In some preferred embodiments, the organic acid comprises citric acid or glacial acetic acid.

Preferably, the organic acid is citric acid.

The applicant has surprisingly found that when citric acid is added in a specific ratio, the stability of the gibberellic acid after spraying can be improved to a certain extent, so that the gibberellic acid can play a long-acting role.

In another aspect of the present invention, there is provided a method for preparing a cell division promoter, comprising the steps of:

(1) firstly, adding a solvent into a stirring kettle, pouring solid raw materials of benzylaminopurine and gibberellic acid into the same stirring kettle, and stirring until the benzylaminopurine and the gibberellic acid are completely dissolved;

(2) and after stirring is finished, sampling, and detecting to be qualified through a laboratory to obtain the product.

Has the beneficial effects that: the invention takes the benzylaminopurine and the gibberellic acid with a specific ratio as active ingredients, remarkably improves the grape fruit setting rate, improves the grape yield by more than 10 percent, and simultaneously adopts a specific solvent to increase the stability of the prepared cell division promoting agent and effectively avoids the generation of precipitates in a system. In addition, the special citric acid is added, so that the stability of the gibberellic acid after spraying can be enhanced, and the gibberellic acid can play a long-acting role.

Detailed Description

Example 1

The embodiment provides a cell division promoter which comprises 1.8 parts of benzylaminopurine, 1.8 parts of gibberellic acid, 58 parts of ethanol, 9 parts of dimethylacetamide, 6 parts of citric acid and purified water in parts by weight, wherein the balance is up to 100 parts.

In another aspect of this embodiment, there is provided a method for preparing a cell-division promoting agent, comprising the steps of:

(1) pumping purified water, dimethylacetamide, citric acid and ethanol into a stirring kettle by a gear pump in sequence, pouring the solid raw materials of the benzylaminopurine and the gibberellic acid into the same stirring kettle, and stirring for 90 minutes until the benzylaminopurine and the gibberellic acid are completely dissolved;

(2) and after stirring is finished, sampling, and detecting to be qualified through a laboratory to obtain the product.

Example 2

The embodiment of the present invention provides a cell division promoter and a preparation method thereof, and the specific implementation manner is the same as that of embodiment 1, except that the weight ratio of ethanol, dimethylacetamide and water is 4.5: 1: 4.2.

example 3

The present example provides a cell division-promoting agent and a method for preparing the same, and the specific embodiment is the same as example 1, except that ethanol is replaced by isopropanol.

Performance testing

The cell division promoters prepared in examples 1 to 3 were left at 10 ℃ for 48 hours, and whether or not a precipitate was precipitated was observed, and the results are shown in Table 1.

TABLE 1

Example numbering	After standing state
		Example 1	No precipitation
Example 2	A small amount of precipitate is separated out
		Example 3	There is more precipitate

Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can make modifications and changes based on the above description, and all such modifications and changes should fall within the scope of the appended claims.

Claims (10)

1. The cell division promoter is characterized by comprising an active ingredient and a solvent, wherein the active ingredient comprises benzylaminopurine and gibberellic acid.

2. The agent for promoting cell division according to claim 1, comprising 2 to 6 parts by weight of the active ingredient, the solvent being supplemented to 100 parts by weight.

3. The agent for promoting cell division according to claim 1 or 2, wherein the weight ratio of benzylaminopurine to gibberellic acid is (0.5-1.5): 1.

4. the agent for promoting cell division according to claim 1 or 2, wherein the solvent comprises one or more of organic alcohol, amide solvent, and water.

5. The mitogen according to claim 4, wherein the organic alcohol is a hydroxyl-terminated alcohol.

6. The mitogen-assisted agent of claim 5, wherein the hydroxyl-terminated alcohol comprises ethanol.

7. The agent for promoting cell division according to claim 4, wherein the amide-based solvent has a viscosity of 0.8 to 1mpa.s at 25 ℃.

8. The mitogen according to claim 2, wherein the mitogen further comprises an organic acid.

9. The mitogen according to claim 8, wherein the organic acid comprises citric acid or glacial acetic acid.

10. A method for preparing a cell division-promoting agent according to any one of claims 1 to 9, comprising the steps of:

(1) firstly, adding a solvent into a stirring kettle, pouring solid raw materials of benzylaminopurine and gibberellic acid into the same stirring kettle, and stirring until the benzylaminopurine and the gibberellic acid are completely dissolved;

(2) and after stirring is finished, sampling, and detecting to be qualified through a laboratory to obtain the product.