CN115094019A

CN115094019A - Preparation method and application of algae-removing compound microbial agent

Info

Publication number: CN115094019A
Application number: CN202210944446.4A
Authority: CN
Inventors: 李申; 李泽典; 魏东霞; 邱国勤; 陈文超; 程谦勋; 孟令鑫
Original assignee: Anhui Jiuwu Tianhong Environmental Protection Technology Co ltd
Current assignee: Anhui Jiuwu Tianhong Environmental Protection Technology Co ltd
Priority date: 2022-08-03
Filing date: 2022-08-03
Publication date: 2022-09-23

Abstract

The invention discloses a preparation method of a algae-removing compound microbial agent, which comprises the following steps of carrying out expanded culture on activated algae-dissolving bacteria, flocculation bacteria and beneficial bacteria powder; respectively centrifuging the culture bacteria liquid to obtain effective bacteria; respectively freeze-drying the obtained algicidal bacterium effective bacteria, flocculating bacterium effective bacteria and beneficial bacterium powder effective bacteria; mixing the algae-lysing bacteria and the flocculating bacteria after drying to obtain a component I; mixing the beneficial bacterium powder with nutrient elements to obtain a component II; before the compound microbial agent is used, the component I and the component II are dissolved and soaked in water for no less than 2 hours for activation. But also can adhere to the surface of the algae cells to prevent the algae cells from performing photosynthesis to accelerate the death of the algae cells, or directly contact the algae cells to release sterilizing substances, thereby achieving the effect of eliminating the algae.

Description

Preparation method and application of algae-removing compound microbial agent

Technical Field

The invention relates to the technical field of sewage treatment, in particular to a preparation method and application of an algae removal compound microbial agent.

Background

About 70 percent of natural water bodies in China are in a eutrophication state, and the nutrient sources of the natural water bodies are very complex and comprise agricultural non-point sources, urban point sources, water body internal sources, atmospheric sedimentation and the like. On the other hand, with the water bodies artificially constructed in artificial lakes, landscape ponds and the like, the related ecological systems are imperfect, so that the self-repairing capability is weak, the water quality is more easily deteriorated, the excessive propagation of algae occurs, and the water quality is deteriorated. In summer, when the temperature is high, the nutrient substances in the water body, the high temperature and the strong light provide conditions for the rapid growth and the propagation of the algae.

At present, chemical agents are most commonly added to kill algae in the market, although the chemical agents have quick effect and good effect. But not only can not radically cure the algae and cause the relapse of the algae with lost drug property, but also can kill the algae and simultaneously has toxic action on other aquatic organisms. Meanwhile, the chemical treatment can lead the cyanobacteria cells to be cracked and release algal toxins, thereby causing the water quality problem to be further worsened and influencing the structure and the function of the whole ecological system; and the current culture mode has low vibration efficiency and is not suitable for culturing a plurality of different strains.

Disclosure of Invention

The invention aims to solve the problems of the background technology and provides a preparation method and application of an algae-removing compound microbial agent. But also can adhere to the surface of the algae cells to prevent the algae cells from performing light action and accelerate the death of the algae cells, or directly contact the algae cells to release bactericidal substances, thereby achieving the effect of eliminating the algae.

The purpose of the invention can be realized by the following technical scheme:

a preparation method of a algae-removing compound microbial agent comprises the following steps:

step 1: respectively activating algicidal bacteria, flocculating bacteria and beneficial bacteria powder;

step 2: carrying out enlarged culture on the activated algicidal bacteria, flocculating bacteria and beneficial bacteria powder, and respectively carrying out shake culture for 3-7 days at the temperature of 28 +/-2 ℃ to obtain a large amount of culture bacteria liquid;

and step 3: respectively centrifuging the culture solution, suspending with deionized water, centrifuging again, repeating the above steps, and centrifuging for 3-4 times to obtain effective bacteria;

and 4, step 4: respectively freeze-drying the obtained algicidal bacterium effective bacteria, flocculation bacterium effective bacteria and beneficial bacterium powder effective bacteria;

and 5: mixing the algae-lysing bacteria and the flocculating bacteria after drying to obtain a component I;

step 6: mixing the beneficial bacterium powder with nutrient elements to obtain a component II;

and 7: before the compound microbial agent is used, the component I and the component II are respectively dissolved and soaked in water for not less than 2 hours according to the proportion of 1:10-30, and then activation is carried out.

As a further scheme of the invention: in step 1, the activated bacteria culture solution comprises the following raw materials by weight: 1g of beef extract, 15g of peptone, 5g of sodium chloride, 20g of glucose and 1L of distilled water; and the pH of the culture solution is 7.4-7.6.

As a further scheme of the invention: in the step 5, mixing the algicidal bacteria and the flocculating bacteria according to the mass ratio of 1-3: 1.

As a further scheme of the invention: in step 6, mixing the beneficial bacterium powder and the nutrient elements according to the mass ratio of 10-50: 1-3, mixing.

As a further scheme of the invention: in the step 1, the algae-lysing bacteria are one or more of pseudomonas, myxobacteria, alteromonas, enterobacter, fiber-phagocytosis bacteria, vibrio and flexual bacteria;

the flocculation bacteria is one or more of bacillus mucilaginosus, putrefying bacteria, bacillus megaterium and nitrogen monad;

the beneficial bacteria is at least one of Bacillus subtilis, Bacillus licheniformis, Clostridium butyricum, yeast, actinomycetes, lactobacillus, photosynthetic bacteria and nitrobacteria.

As a further scheme of the invention: in step 6, the nutrient substances are one or more of trace elements, inorganic salts, carbon sources and vitamins.

As a further scheme of the invention: in the step 2, the algicidal bacteria, the flocculating bacteria and the beneficial bacteria powder are cultured by a vibration device;

the working process of the vibration device is as follows: the telescopic work of the control cylinder drives the connecting shaft to reciprocate along the sliding groove, the connecting shaft drives the transverse plate to reciprocate, and the transverse plate drives the substrate to continuously rotate back and forth along the square frame through the movable plate, so that the conical bottle filled with the strain cells on the substrate is shaken.

As a further scheme of the invention: the vibrating device further rotates through the control transmission shaft, so that the connecting sleeve moves along the transmission shaft, the connecting sleeve drives the moving plate to move along the base plate through the connecting plate, the first limiting groove and the second limiting groove are arranged, the limiting shaft is moved and adjusted, the adjusting plate is driven to move and adjust, the distance between the adjusting plates is changed, and the conical bottle is clamped and fixed.

The application of the algae-removing compound microbial agent obtained by the preparation method is characterized in that the compound microbial agent is suitable for removing and preventing algae in natural water and starts to be used at 9-11 am.

The invention has the beneficial effects that:

(1) firstly, the method is not only suitable for water environments with algal outbreaks such as water bloom, red tide and the like, but also suitable for water environments without algal cell pollution, and is used for preventing and inhibiting mass propagation of algal cells; secondly, not only can remove the algae in the water body, but also can effectively reduce the eutrophication degree of the water body, quickly improve the water quality and provide the water body with transparency; thirdly, the algae removal agent is suitable for most algae in natural water, and has the advantages of quick response, high algae removal rate, safety, no pollution and no secondary pollution; fourthly, the use is convenient, the operation is simple and the cost is low; fifth, there is no side effect, not only will not cause the injury to the aquatic animal in the application range, the microorganism bacterium can be digested and absorbed as the food of protozoa and aquatic animal, favorable to the stability of the ecosystem, economic benefits are good;

(2) the arrangement of the transverse plate in the vibrating mechanism of the invention leads the conical bottles in a row and a plurality of rows to be driven to vibrate by one cylinder, thereby greatly improving the cell culture quantity of different strains; the fixing work of all the conical bottles on each row of the base plates can be completed only by operating the driving motor, and meanwhile, the driving mechanism can control the distance between the adjusting plates, so that the conical bottles with different sizes can be fixed.

Drawings

The invention will be further described with reference to the accompanying drawings.

FIG. 1 is a block flow diagram of the present invention;

FIG. 2 is a schematic view of the construction of the vibration device of the present invention;

FIG. 3 is a schematic view of the construction of the vibrating mechanism of the present invention;

FIG. 4 is a schematic structural view of the drive mechanism of the present invention;

FIG. 5 is a schematic view of the structure of the substrate of the present invention;

FIG. 6 is a schematic view of the connection between the base plate and the adjustment plate according to the present invention;

FIG. 7 is a graph showing the variation of the density of different algae in the same dose according to the present invention;

FIG. 8 is a graph showing the variation in Chlorella algal density at different dosages of the present invention;

FIG. 9 is a schematic illustration of the contaminant removal rate of the present invention;

FIG. 10 is a graph showing the contaminant removal rate of the present invention.

In the figure: 1. a square frame; 2. a side plate; 3. a cylinder; 4. a connecting shaft; 5. a transverse plate; 6. a movable plate; 7. a substrate; 8. a splint; 9. a clamping groove; 10. a drive shaft; 11. connecting sleeves; 12. a connecting plate; 13. moving the plate; 14. a first limit groove; 15. a second limit groove; 16. a limiting shaft; 17. an adjusting plate; 18. a first limit plate; 19. a first stop collar; 20. a second limiting plate; 21. and a second stop collar.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

Referring to fig. 1, the present invention relates to a preparation method and an application of a composite microbial agent for algae removal, which specifically comprises the following steps:

step 1: activating a microbial inoculum;

activating and culturing commercially available algicidal bacteria, flocculation bacteria and beneficial bacteria at 26 deg.C for 1 day;

and (3) bacteria culture solution: 1g of beef extract, 15g of peptone, 5g of sodium chloride, 20g of glucose and 1L of distilled water, and the pH value is 7.4.

Step 2: expanding culture

The culture was carried out at 26 ℃ for 3 days with shaking to obtain a large amount of culture solution. Respectively centrifuging the bacterial liquid at 2000r/min for 10min, removing supernatant, suspending with deionized water, centrifuging again, repeating the process, and centrifuging for 3-times to obtain effective bacteria;

and step 3: respectively carrying out freeze drying on culture bacteria liquid of algae-lysing bacteria, flocculating bacteria and beneficial bacteria;

and 4, step 4: mixing the dried algicidal bacteria and flocculating bacteria according to the mass ratio of 1:1 to obtain a component I;

and 5: beneficial bacterium powder, enzyme activity factors and nutrient elements are mixed according to the mass ratio of 10: 1:1 to obtain a component II.

Wherein, the algae-lysing bacteria in the step 1 are preferably one or more of pseudomonas, myxobacteria and enterobacter; the flocculation bacteria is preferably one or more of bacillus mucilaginosus, putrefying bacteria, bacillus megaterium and nitrogen monad; the beneficial bacteria are preferably at least one of bacillus subtilis, yeast, actinomycetes, lactic acid bacteria and photosynthetic bacteria;

in the step 2, the bacterial density of the bacterial liquid is not less than 10 ⁸ Per ml;

in the step 5, the nutrient substances are one or more of trace elements, inorganic salts, carbon sources and vitamins;

the effective viable count of the component one is not less than 3.0 hundred million/g.

The effective viable count of the component two is not less than 2.0 hundred million/g.

The compound microbial agent is suitable for removing and preventing algae in natural water, and preferably begins to be used at about 9-11 am;

when the compound microbial agent is used, oxygen aeration is required;

before use, the first component and the second component of the compound microbial agent are dissolved and soaked in water for no less than 2 hours according to the ratio of 1:10 respectively;

the compound microbial agent is preferably used by firstly using the component I, and then using the component II after 2-6 hours;

the dosage of the component one is 0.1g/m ³ ；

The adding amount of the second component is 0.2g/m ³ 。

Example 2

Based on the above embodiment 1, the difference from the embodiment 1 is that:

step 1: activating the microbial inoculum;

activating and culturing commercially available algicidal bacteria, flocculation bacteria and beneficial bacteria at 28 deg.C for 2 days;

and (3) bacteria culture solution: 1g of beef extract, 15g of peptone, 5g of sodium chloride, 20g of glucose and 1L of distilled water, and the pH value is 7.5.

And 2, step: expanding culture

The culture was performed for 5 days at 28 ℃ with shaking to obtain a large amount of culture solution. Respectively centrifuging the bacterial liquid at 2000r/min for 10min, removing supernatant, suspending with deionized water, centrifuging again, repeating the process, and centrifuging for 3 times to obtain effective bacteria;

and 4, step 4: mixing the dried algicidal bacteria and flocculating bacteria according to the mass ratio of 2:1 to obtain a component I;

and 5: beneficial bacterium powder, enzyme activity factors and nutrient elements are mixed according to the mass ratio of 30: 1:2 to obtain a component II;

before use, the first component and the second component of the compound microbial agent are dissolved and soaked in water for no less than 2 hours according to the proportion of 1:20 respectively;

administration of component oneThe addition amount is 0.2g/m ³ ；

The adding amount of the second component is 0.3g/m ³ 。

Example 3

Based on the above embodiment 1, the difference from the embodiment 1 is that:

activating and culturing commercially available algicidal bacteria, flocculation bacteria and beneficial bacteria at 30 deg.C for 3 days;

and (3) bacteria culture solution: 1g of beef extract, 15g of peptone, 5g of sodium chloride, 20g of glucose and 1L of distilled water, and the pH value is 7.6.

Step 2: expanding culture

The culture medium was cultured at 30 ℃ for 7 days with shaking to obtain a large amount of culture medium. Respectively centrifuging the bacterial liquid at 2000r/min for 10min, removing supernatant, suspending with deionized water, centrifuging again, repeating the process, and centrifuging for 4 times to obtain effective bacteria;

and 4, step 4: mixing the dried algicidal bacteria and flocculating bacteria according to the mass ratio of 3:1 to obtain a component I;

and 5: beneficial bacterium powder, enzyme activity factors and nutrient elements are mixed according to the mass ratio of 50: 1:3 to obtain a component II;

before use, the first component and the second component of the compound microbial agent are dissolved and soaked in water for no less than 2 hours according to the proportion of 1:30 respectively;

the dosage of the component one is 0.3g/m ³ ；

The adding amount of the second component is 0.4g/m ³ 。

Example 4

Five kinds of algae cultured under laboratory culture conditions were tested, and all the algae were purchased from the freshwater algae seed bank of Wuhan aquatic institute. Experiments are carried out under natural light conditions in a laboratory, and the density of experimental algae is 10 ⁵ Each volume/ml, the experimental algae liquid is 5L, and the algae density is the density of the artificially-performed algae. The adding amount of the first component of the algae removal compound microbial agent is 0.2g/m ³ Adding the component II 4 hours after the component I is added, wherein the adding amount of the component II is 0.3g/m ^3， The experimental results are shown in fig. 7;

example 5

The laboratory culture of chlorella is carried out under natural light conditions, and the density of the experimental algae is 10 ⁵ The cell count/mL, the experimental algae solution is 5L, and the algae density is artificially counted. Adding the components 10 a.m., wherein the adding doses of the algae-removing compound microbial agent are different, and adding the component two 4h after the component one is added;

the dosage of the component one is 0.1g/m ³ The adding amount of the component two is 0.2g/m ³ And is marked as "1 + 2";

the dosage of the component one is 0.1g/m ³ The adding amount of the component two is 0.4g/m ³ And is marked as "1 + 4";

the dosage of the component one is 0.2g/m ³ The adding amount of the component two is 0.3g/m ³ Marked as "2 + 3";

the dosage of the component one is 0.3g/m ³ The adding amount of the component two is 0.2g/m ³ And is marked as "3 + 2";

the dosage of the component one is 0.3g/m ³ The adding amount of the component two is 0.4g/m ³ And is marked as "3 + 4";

the results of the experiment are shown in FIG. 8.

Example 6

The field of the artificial lake project with the algae outbreak is subjected to the algae removal experiment, the area of the artificial lake is 9000 square meters, and the water depth is 1.5-1.8 m. Before treatment, water quality detection is carried out, and the monitoring result is COD: 42mg/L, chlorophyll a: 107.3ug/L, TN: 4.9mg/L, ammonia nitrogen: 2.1mg/L, TP: 0.57 mg/L. At 10 am, the adding amount of the component I on the water surface is 0.2g/m ³ The adding amount of the component two is 0.3g/m ³ And (4) throwing the compound microbial agent, and adding the two components at an interval of 4 hours. The water quality detection of the experimental water sample for one month is carried out, and the results are as follows: the experimental results are shown in fig. 9;

example 7

Example 4 was carried out in a river around the project area, with a river water area of 5000 square meters and a water depth of 1.0 to 1.5 m. Dividing into three sections, and adding the first component into one section of water area at a dosage of 0.5g/m ³ Adding the second component into the second section of water area independently, wherein the adding amount is 0.5 g-m ³ The third section of water area comprises 0.2g/m of the component ³ And component two 0.3g/m ³ Adding the raw materials; the results of the experiment are shown in FIG. 10.

Based on the above examples 1-5, algicidal bacteria: inhibiting the growth of algae, or killing algae, in a direct or indirect manner, by direct contact with algae lysing or releasing some highly active algae lysing substance.

Flocculating bacteria: the secretion of flocculation substance can not only form bacterial gel film on algae cell to prevent photosynthesis of algae cell, but also facilitate the adsorption of suspended particle.

Beneficial bacteria: through the metabolism activity of the sewage treatment device, organic substances in the sewage are desorbed and absorbed, the eutrophication degree of the water body is reduced, meanwhile, the growth of mixed bacteria is inhibited, and the water body environment is balanced.

The algae-removing compound microbial agent is suitable for water environments with algae outbreaks such as water bloom, red tide and the like, is suitable for preventing and inhibiting mass propagation of algae cells without causing algae cell pollution; secondly, the algae in the water body can be removed, the eutrophication degree of the water body can be effectively reduced, the water quality can be rapidly improved, and the transparency of the water body can be provided; thirdly, the algae removal agent is suitable for most algae in natural water, takes effect quickly, has high algae removal rate, is safe and pollution-free, and does not cause secondary pollution; fourthly, the use is convenient, the operation is simple, and the cost is low; fifthly, the microbial fertilizer has no side effect, does not cause harm to aquatic animals in the use range, can be digested and absorbed as food of protozoa and aquatic animals, is beneficial to the stability of an ecological system, and has good economic benefit.

Example 8

Based on the above example 1, in the step 2, the algae-lysing bacteria, the flocculating bacteria and the beneficial bacteria are cultured, but the current culture mode has low vibration efficiency and is not suitable for culturing a plurality of different strains; the following vibration devices were used;

the vibrating device comprises a square frame 1 and a substrate 7; the square frame 1 is vertically arranged, the substrate 7 is movably arranged in the square frame 1, the conical flasks are arranged in the substrate 7, the substrates 7 are arranged in parallel along the vertical height of the square frame 1, the vibration mechanism is arranged on the square frame 1 and connected with the substrates 7 through the vibration mechanism, and the vibration mechanism is controlled to work to drive the substrates 7 to shake simultaneously, so that the activated algae cells are subjected to enlarged vibration culture;

the vibrating mechanism comprises a side plate 2, a cylinder 3, a connecting shaft 4, transverse plates 5 and a movable plate 6, wherein the side plate 2 is installed on the side wall of a square frame 1, the cross section of the side plate 2 is of an L-shaped structure, the cylinder 3 is movably installed on the horizontal plane of the side plate 2, the output end of the cylinder 3 is connected with the connecting shaft 4, a sliding groove matched with the connecting shaft 4 is formed in the vertical plane of the side plate 2, one transverse plate 5 is arranged at one end, away from the cylinder 3, of the connecting shaft 4, another transverse plate 5 is arranged at one side, away from the side plate 2, of the square frame 1, the movable plate 6 is installed between the two transverse plates 5, the middle part of the movable plate 6 is rotatably installed on the inner wall of the square frame 1, and one side, away from the transverse plate 5, of the movable plate 6 is connected with the movable plate 6;

the cylinder 3 is vertically arranged with the output end of the connecting shaft 4, and the connecting shaft 4 penetrates through the sliding groove of the side plate 2 and moves along the sliding groove;

when the vibrating mechanism works, the cylinder 3 is controlled to stretch and retract to drive the connecting shaft 4 to reciprocate along the sliding groove, the connecting shaft 4 drives the transverse plate 5 to reciprocate, the transverse plate 5 drives the substrate 7 to ceaselessly reciprocate along the square frame 1 through the movable plate 6, so that the conical bottles filled with strain cells on the substrate 7 can be subjected to vibrating culture, and the transverse plate 5 in the vibrating mechanism is arranged to drive one row of a plurality of conical bottles and a plurality of rows of conical bottles to vibrate, so that the number of different strain cells to be cultured is greatly increased;

example 9

Based on the embodiment 8, when the vibration device is used for installing and placing the conical flasks in the early stage and taking the conical flasks after the culture is finished, the conical flasks need to be placed in the fixing groove one by one and fixed, and after the culture is finished, the conical flasks need to be taken down by opening the fixing structure one by one, so that the operation is repeated, and the workload is large;

therefore, a fixing mechanism is arranged on the base plate 7, and the fixing mechanism comprises a clamping plate 8 and a clamping groove 9;

the clamping plates 8 are arranged side by side, the clamping plate 8 in the middle is fixed in the middle of the base plate 7, the clamping plates 8 on two sides can horizontally move along the base plate 7, the arc-shaped clamping grooves 9 are respectively arranged on two sides of the middle of the clamping plates 8, the clamping plates 8 are connected with a driving mechanism, and the driving mechanism can adjust the distance between the clamping plates 8, so that the effect of fixing the conical bottles is achieved;

the driving mechanism is positioned on the side wall of the base plate 7 far away from the clamping plate 8, so that the clamping plate 8 and the driving mechanism are separately arranged, and the problems of blockage and space occupation can be avoided when the conical flask is placed;

the driving mechanism comprises a transmission shaft 10, a connecting sleeve 11, a connecting plate 12, a moving plate 13, a first limiting groove 14, a second limiting groove 15, a limiting shaft 16 and an adjusting plate 17;

the transmission shaft 10 is rotatably installed on one side of the base plate 7, the transmission shaft 10 is sleeved with a connecting sleeve 11, the transmission shaft 10 is in threaded connection with the connecting sleeve 11, a connecting plate 12 is sleeved on the connecting sleeve 11, one end, far away from the connecting sleeve 11, of the connecting plate 12 is connected with a movable plate 13, the movable plate 13 is in sliding connection with the base plate 7 through a second limiting piece, a first limiting groove 14 and a second limiting groove 15 are formed in the movable plate 13, the first limiting groove 14 is located in the middle of the movable plate 13 and is linearly arranged, a plurality of second limiting grooves 15 are symmetrically arranged along the first limiting groove 14 and are obliquely arranged, a plurality of adjusting plates 17 are arranged between the movable plate 13 and the base plate 7 side by side, limiting shafts 16 are arranged on the adjusting plates 17, the limiting shafts 16 are respectively correspondingly located in the first limiting groove 14 and the second limiting groove 15, and the adjusting plates 17 are connected with the clamping plate 8;

wherein, the transmission shaft 10 is a screw rod, one end of the transmission shaft 10 is connected with the output end of a driving motor, and the driving motor is arranged on the substrate 7;

the adjusting plate 17 is connected with the base plate 7 in a sliding manner through a first limiting piece, the first limiting piece comprises a first limiting plate 18 and a first limiting sleeve 19, the first limiting plate 18 is horizontally arranged on the base plate 7, the first limiting sleeve 19 is sleeved on the first limiting plate 18, and the first limiting sleeve 19 is arranged on the adjusting plate 17;

the second limiting piece comprises a second limiting piece 20 and a second limiting sleeve 21, the second limiting piece 20 is horizontally arranged on the base plate 7, the second limiting sleeve 21 is sleeved on the second limiting piece 20, and the second limiting sleeve 21 is arranged on the movable plate 13;

the first limiting piece and the second limiting piece are arranged in a mutually perpendicular relationship, the moving plate 13 is controlled to move along the Y-axis direction, and the adjusting plate 17 moves along the X-axis direction;

when the taper bottle clamping device works, the transmission shaft 10 is controlled to rotate, so that the connecting sleeve 11 moves along the transmission shaft 10, the connecting sleeve 11 drives the moving plate 13 to move along the base plate 7 through the connecting plate 12, and when the moving plate 13 moves, the limiting shaft 16 moves and is adjusted through the arranged first limiting groove 14 and the second limiting groove 15, so that the adjusting plates 17 are driven to move and adjust, the distance between the adjusting plates 17 is changed, and the taper bottle clamping and fixing and later taking work are completed; therefore, the fixing mechanism can complete the fixing work of all the conical bottles on each row of the base plates 7 only by operating the driving motor, and simultaneously, the driving mechanism can control the distance between the adjusting plates 17, so that the conical bottles with different sizes can be fixed;

the working process of the vibrating device of the invention is as follows:

step 1: the connecting sleeve 11 moves along the transmission shaft 10 by controlling the transmission shaft 10 to rotate, the connecting sleeve 11 drives the moving plate 13 to move along the base plate 7 through the connecting plate 12, and when the moving plate 13 moves, the limiting shaft 16 moves and is adjusted through the arranged first limiting groove 14 and the second limiting groove 15, so that the adjusting plates 17 are driven to move and be adjusted, the distance between each adjusting plate 17 is changed, and the conical bottle is clamped and fixed;

and 2, step: the control cylinder 3 is controlled to stretch and retract to drive the connecting shaft 4 to reciprocate along the sliding groove, the connecting shaft 4 drives the transverse plate 5 to reciprocate, and the transverse plate 5 drives the substrate 7 to ceaselessly reciprocate along the square frame 1 through the movable plate 6, so that the conical flask filled with algae cells on the substrate 7 can be subjected to vibration culture.

Although one embodiment of the present invention has been described in detail, the description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the scope of the invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.

Claims

1. A preparation method of a algae-removing compound microbial agent is characterized by comprising the following steps:

and 4, step 4: respectively freeze-drying the obtained algicidal bacterium effective bacteria, flocculating bacterium effective bacteria and beneficial bacterium powder effective bacteria;

2. The method for preparing the algae-removing compound microbial agent according to claim 1, wherein in the step 1, the activated bacteria culture solution comprises the following raw materials by weight: 1g of beef extract, 15g of peptone, 5g of sodium chloride, 20g of glucose and 1L of distilled water; and the pH of the culture solution is 7.4-7.6.

3. The preparation method of the algae removal compound microbial inoculant according to claim 1, wherein in the step 5, the algae dissolving bacteria and the flocculating bacteria are mixed according to the mass ratio of 1-3: 1.

4. The preparation method of the algae-removing compound microbial agent according to claim 1, wherein in the step 6, the mass ratio of beneficial bacterium powder to nutrient elements is 10-50: 1-3, mixing.

5. The method for preparing the algicidal compound microbial agent according to claim 1, wherein the algicidal bacteria in step 1 are one or more of pseudomonas, myxobacteria, alteromonas, enterobacter, cellulophaga, vibrio and flexual bacteria;

6. The method for preparing the algae-removing compound microbial inoculant according to claim 1, wherein in the step 6, the nutrient substances are one or more of trace elements, inorganic salts, carbon sources and vitamins.

7. The method for preparing the algae removal compound microbial agent according to claim 1, wherein in the step 2, the algae dissolving bacteria, the flocculating bacteria and the beneficial bacteria powder are cultured by a vibration device;

the working process of the vibration device is as follows: the control cylinder (3) is controlled to stretch and retract to drive the connecting shaft (4) to reciprocate along the sliding groove, the connecting shaft (4) drives the transverse plate (5) to reciprocate, the transverse plate (5) drives the substrate (7) to ceaselessly rotate back and forth along the square frame (1) through the movable plate (6), and the conical flask which is provided with the strain cells and arranged on the substrate (7) is made to shake.

8. The preparation method of the algae-removing compound microbial inoculant according to claim 7, wherein the vibration device further controls the transmission shaft (10) to rotate, so that the connecting sleeve (11) moves along the transmission shaft (10), the connecting sleeve (11) drives the moving plate (13) to move along the base plate (7) through the connecting plate (12), and the limiting shaft (16) is moved and adjusted by using the first limiting groove (14) and the second limiting groove (15), so that the adjusting plates (17) are driven to move and adjust, the distance between the adjusting plates (17) is changed, and the conical flask is clamped and fixed.

9. The application of the algae-removing compound microbial agent obtained by the preparation method according to claim 1, wherein the compound microbial agent is suitable for removing and preventing algae in natural water and is used at 9-11 am.