CN115074224A - Tissue homogenizer for primary muscle cell culture of livestock - Google Patents

Abstract

The invention discloses a tissue homogenizer for primary muscle cell culture of livestock, which relates to the field of cell culture, and adopts the technical scheme that the tissue homogenizer comprises a bottom platform, wherein the bottom platform comprises a plurality of vertically arranged lower supporting rods, the bottom platform is connected with an upper support through the lower supporting rods, the upper support comprises a plurality of guide rods corresponding to the lower supporting rods, and the bottom ends of the guide rods are spliced with the top ends of the lower supporting rods; the top end of the guide rod is provided with a horizontal bearing plate, and the lower side of the bearing plate is provided with an extrusion block; the guide rod is connected with the homogenate component in a sliding way. The invention has the beneficial effects that: the device uses the container with the adjustable bottom as the carrier of the muscle granules, and combines the spherical extrusion block to realize the comprehensive extrusion of the muscle granules by adjusting the state of the bottom of the container, and furthest ensures that the muscle cells are not damaged in the extrusion process.

Description

Tissue homogenizer for primary muscle cell culture of livestock

The scheme is a divisional application, and the original application name is as follows: a tissue homogenizer for culturing primary muscle cells of livestock is disclosed as the application date: 2020-12-15, the application number of the original application is: 202011480262.4.

Technical Field

The invention relates to the field of cell culture equipment, in particular to a tissue homogenizer for culturing primary muscle cells of livestock.

Background

In the primary muscle research of livestock, muscle cell culture is a very important link, after collected livestock muscle tissues are brought back to a laboratory, the tissues are firstly washed for several times by using normal saline or PBS (phosphate buffered saline) liquid in a super clean bench and then cut into tissue grains by using ophthalmic scissors, and the size of the tissue grains can not be larger than 0.5mm ³ Otherwise, the strain is inoculated to a culture bottle or a culture dish, and is easy to float after the culture solution is added, and cannot grow adherent. In operation, we find that the muscle tissue is cut to be so small with scissors, which is time-consuming and labor-consuming, and has uneven tissue grain size. Due to the uneven size of the tissue particles, the time for adding the culture solution after the tissue particles are inoculated into a culture bottle or a culture dish is not easy to control. If it is observed that small tissue particles are already attached to the wall, then the larger tissue particles are easily floated when the culture solution is added; if larger tissue particles are observed to adhere, at which time culture fluid is added, the smaller tissue particles are likely to lose viability due to desiccation, resulting in an inability to proliferate.

Disclosure of Invention

Aiming at the technical problem, the invention provides a tissue homogenizer for culturing primary muscle cells of livestock.

The technical scheme is that the device comprises a bottom table, wherein the bottom table comprises a plurality of vertically arranged lower support rods, the bottom table is connected with an upper support through the lower support rods, the upper support comprises a plurality of guide rods corresponding to the lower support rods, and the bottom ends of the guide rods are inserted into the top ends of the lower support rods; the top end of the guide rod is provided with a horizontal bearing plate, and the lower side of the bearing plate is provided with an extrusion block;

the guide rod is connected with a pulp homogenizing assembly in a sliding mode, and the pulp homogenizing assembly comprises an outer barrel and an inner barrel which are sleeved; the outer cylinder and the inner cylinder are cylindrical cylinders, the outer cylinder and the inner cylinder are hollow, the peripheral walls of the outer cylinder and the inner cylinder are made of rigid materials, the top end of the outer cylinder is open, a top film is arranged at the top end of the inner cylinder, the top film is made of inextensible flexible materials, and the natural state of the top film is hemispherical; a liquid inlet is formed at the bottom end of the inner cylinder, and the inner cavity of the inner cylinder is communicated with the bottom end inside a liquid box arranged in the base platform through a liquid inlet pipeline and a liquid pump;

and a driving assembly for driving the homogenate component to vertically slide along the guide rod is further arranged in the bottom table.

Preferably, the liquid inlet pipeline is a flexible corrugated pipe;

preferably, the liquid in the inner cylinder and the liquid box is hydraulic oil;

when the device is used, primary muscle grains of the livestock taken in advance are placed into the homogenate component, and the guide rod of the upper support is in butt joint with the lower support. The inner cylinder is full of liquid in an initial state, the top film is enabled to be protruded into a hemispherical shape, and the homogenate component moves upwards to be contacted with the extrusion block under the action of the driving component, so that the muscle particles are extruded and changed into smaller particles.

After the extrusion is finished, part of the liquid in the inner cylinder is extracted, and the volume V of the extracted liquid conforms to the formula:

wherein r is equal to the radius of the inner ring of the top end face of the inner cylinder;

then, the drive assembly is used for lifting the homogenate component again, so that the extrusion block is in contact with the top membrane, and the muscle granules are secondarily extruded by combining the folds of the top membrane which are changed from a spherical surface into a plane under the thrust given by the drive assembly.

After finishing the secondary extrusion, take the volume of volume V out with the liquid in the inner tube once more, the apical membrane becomes the sphere of indent this moment, repeats the step that promotes the homogenate subassembly to the realization is to the cubic extrusion of muscle grain.

The process is repeated for a plurality of times, so that the muscle particle homogenization process can be completed, the muscle particle homogenization process is changed from granular state to pasty state, the pasty state can be better attached to the inner wall of the culture container, the inoculation can be better performed, the influence of the culture solution injection process is not easy to occur after the inoculation of the pasty state, and the improvement of the cell culture quality is facilitated.

Preferably, the extrusion block includes a spherical block corresponding to an inner diameter of the top end of the inner cylinder. The spherical block can be better attached to the concave state of the top film, so that a better slurry homogenizing effect is obtained.

Preferably, a closed cylinder is arranged on the periphery of the spherical block of the extrusion block, the inner diameter of the closed cylinder corresponds to the outer diameter of the outer cylinder, and a sealing ring is arranged at the lower end of the closed cylinder;

the inner top of the closed cylinder is positioned above the horizontal section of the spherical block, and the horizontal section is a horizontal section passing through the spherical center of the spherical block;

the horizontal plane where the bottom end of the closed cylinder is located below the bottom end face of the spherical block.

Through sealing the section of thick bamboo, when the homogenate subassembly rebound, sealing the section of thick bamboo cover in the urceolus outside, make the air in the sealing section of thick bamboo difficult to discharge through the sealing washer, especially when the apical membrane is in recessed state, because the air is very easy to be compressed, and the less relation of inside air volume, then can not influence normal extrusion homogeneity, and can prevent the complete laminating of globular piece and apical membrane through the air of compressed to avoid muscle grain to be excessively extruded and lead to the destroyed condition of cell.

Preferably, an elastic pad is arranged at the top end of the extrusion block.

When this device uses, in order to guarantee the extrusion pressure degree of extrusion piece, the top of upper bracket is pressed downwards from the top to the user with the hand, presses the position like the outside casing of top motor, when exerting decurrent pressure, thereby because closed cylinder causes its inside air to be difficult to discharge and produce reverse ascending thrust, the cushion can have better buffering effect to this power, and the atress through the cushion contracts and plays the effect of avoiding muscle tissue cell to be excessively extruded and destroy equally.

Preferably, a top motor is fixedly arranged on the upper side of the bearing plate, a motor shaft of the top motor penetrates through the bearing plate to be linked with the spherical block, and the motor shaft of the top motor is coaxial with the spherical block.

Preferably, the closed cylinder and the spherical block are connected through a bearing.

The connection form here can be selected as, the top of a sealed section of thick bamboo and the downside fixed connection of loading board, and globular piece passes through the bearing and seals a section of thick bamboo inner wall connection, and globular piece top is connected with the motor shaft of top motor through the cushion.

The top motor drives the spherical block to rotate or twist reciprocally, and when the spherical block is contacted with muscle grains, a better slurry homogenizing effect can be achieved.

Preferably, horizontal plates are symmetrically arranged on two sides of the lower part of the outer cylinder, and through holes are formed in the horizontal plates and are in sliding connection with the guide rods through the through holes;

the driving assembly comprises a cam positioned below the horizontal plate, and a wheel shaft of the cam is linked with a motor shaft of a lower motor;

the cam comprises a cam body, a cam shaft hole, four outer convex surfaces and a cam body, wherein the wheel body of the cam is provided with the wheel shaft hole, the wheel body is provided with the four outer convex surfaces positioned on the peripheral sides of the wheel shaft hole, each outer convex surface is an arc-shaped surface, and a plane is arranged between every two adjacent outer convex surfaces;

a connecting line from the circle center of the axle hole to the farthest point of each outer convex surface is in a cross shape, and the length of the connecting line from the circle center of the axle hole to the farthest point of each outer convex surface is sequentially increased by the length r in a clockwise or anticlockwise sequence;

r is equal to the radius of the inner ring of the top end face of the inner cylinder;

the length of a connecting line from the center of the axle hole to the far end point of the nearest outer convex surface is equal to r.

Through the structure, the device is in an initial state that the outer convex surface closest to the axle hole of the cam is positioned on the upper side, the bottom surface of the horizontal plate is attached to the outer convex surface, the distance between the bottom end of the spherical block and the top end of the top film in a convex state is r, after the cam rotates by 90 degrees, the horizontal plate is jacked up to move by the distance r, and at the moment, the bottom end of the spherical block is contacted with the top end of the top film to be extruded for one time; when the secondary extrusion is carried out, the top film retracts by a distance r, the cam rotates by 90 degrees again, and the lifting distance of the horizontal plate relative to the initial position is 2 r; by analogy, when the extrusion is carried out for three times, the lifting distance of the horizontal plate relative to the initial position is 3 r. The cam is reset after continuously rotating for 90 degrees, and the operation is repeated.

The structure has the advantages that the structure can be combined with a stepping motor to be used as a cam, and the quantitative lifting of the horizontal plate can be realized by rotating the cam by 90 degrees every time. Compared with a hydraulic and pneumatic push rod, the cam is adopted for quantitative driving, so that the device can run more stably, and the manufacturing cost is far lower than that of a pneumatic and hydraulic driving mode. In addition, the cam is used as a lifting mechanism, and other mechanical structures which move periodically can be combined to be used as a driving structure, so that the common one-way motor can be used for driving.

Preferably, the bottom end of the inner cylinder is fixedly connected with a screw rod, the screw rod is hollow and is communicated with the interior of the inner cylinder, the screw rod is provided with the liquid inlet hole, and the bottom end of the screw rod is connected with the liquid inlet pipeline;

the bottom end of the outer barrel is rotatably connected with an adjusting ring, and the inner side of the adjusting ring is provided with internal threads which are in threaded connection with the screw rod;

the urceolus lower part is provided with vertical spacing groove, the outside lower part of inner tube corresponds the spacing groove and fixedly sets up the stopper, the inner tube pass through stopper and spacing groove with urceolus sliding connection.

Through this structure, after accomplishing the homogeneity, can will rotate the motion of adjustable ring drive lead screw to with the inner tube jack-up that makes things convenient for the staff to take out the meat paste after the homogeneity.

A method for culturing primary muscle cells of livestock comprises the steps of,

s1, obtaining muscle blocks from livestock;

s2, shearing the blocks obtained in the S1 into muscle grains;

s3, rolling the muscle granules of S2 to make the muscle granules into paste;

s4, inoculating the paste obtained in the step S3 into a culture container, and waiting until the paste is attached to the wall completely;

s5, adding a culture solution.

A method of homogenizing primary muscle cells of a domestic animal using the tissue homogenizer of claim 1, comprising the steps of,

a1, putting the primary muscle grains of the livestock taken in advance into a homogenate component, and pumping liquid in the inner cylinder in the initial state to enable the top membrane to be protruded into a hemispherical shape;

a2, making the homogenate component move upwards to contact with the squeezing block to form primary squeezing on the muscle particles;

a3, extracting a part of liquid in the inner cylinder, wherein the volume V of the extracted liquid conforms to the formula:

wherein r is equal to the radius of the inner ring of the top end face of the inner cylinder;

a4, lifting the homogenizing component again to enable the extrusion block to contact with the apical membrane for secondary extrusion of muscle granules;

a5, repeating the steps A3 and A4, and extruding for three times;

a6, repeating the steps A2 to A5 for a plurality of times until the homogenization is completed.

The technical scheme provided by the embodiment of the invention has the following beneficial effects: this device passes through bottom adjustable container as the supporting body of muscle grain, through the state of adjustment container bottom, combines the globular extrusion piece to realize the comprehensive extrusion to the muscle grain, and furthest's assurance extrusion in-process muscle cell is not destroyed.

Drawings

Fig. 1 is a schematic overall structure diagram of an embodiment of the present invention.

Fig. 2 is a schematic view of a hidden housing according to an embodiment of the invention.

Fig. 3 is a schematic structural diagram of an extrusion block according to an embodiment of the present invention.

Fig. 4 is a schematic view of a cam structure according to an embodiment of the present invention.

Fig. 5 is a front view of a cam in accordance with an embodiment of the present invention.

FIG. 6 is a schematic diagram illustrating a protruded state of the top film according to the embodiment of the present invention.

FIG. 7 is a schematic view of a top film in a contracted state according to an embodiment of the present invention.

Wherein the reference numerals are: 1. a base table; 101. a lower support rod; 102. a liquid box; 2. an upper bracket; 210. a guide bar; 220. carrying a plate; 203. extruding the block; 240. a top motor; 231. a spherical block; 232. a closed cartridge; 233. an elastic pad; 3. a homogenizing assembly; 310. an outer cylinder; 311. a horizontal plate; 312. an adjusting ring; 320. an inner barrel; 321. a top film; 322. a liquid inlet pipeline; 323. a screw rod; 4. a drive assembly; 401. a cam; 411. a wheel axle bore; 420. a lower motor.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. Of course, the specific embodiments described herein are merely illustrative of the invention and are not intended to be limiting.

It should be noted that the embodiments and features of the embodiments of the present invention may be combined with each other without conflict.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings, which are merely for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be construed as limiting the invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the invention, the meaning of "a plurality" is two or more unless otherwise specified.

In the description of the invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "connected" and "disposed" are to be construed broadly, e.g. as being fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the creation of the present invention can be understood by those of ordinary skill in the art through specific situations.

Example 1

Referring to fig. 1 to 7, the present invention provides a tissue homogenizer for culturing primary muscle cells of livestock, which comprises a base table 1, wherein the base table 1 comprises a plurality of vertically arranged lower supporting rods 101, the base table 1 is connected with an upper bracket 2 through the lower supporting rods 101, the upper bracket 2 comprises a plurality of guide rods 210 corresponding to the lower supporting rods 101, and the bottom ends of the guide rods 210 are inserted into the top ends of the lower supporting rods 101; the top end of the guide rod 210 is provided with a horizontal bearing plate 220, and the lower side of the bearing plate 220 is provided with a pressing block 203;

the guide rod 210 is connected with a homogenizing component 3 in a sliding way, and the homogenizing component 3 comprises an outer cylinder 310 and an inner cylinder 320 which are sleeved; the outer cylinder 310 and the inner cylinder 320 are cylindrical cylinders, the outer cylinder 310 and the inner cylinder 320 are both hollow, the peripheral walls of the outer cylinder 310 and the inner cylinder 320 are both made of rigid materials, the top end of the outer cylinder 310 is open, the top end of the inner cylinder 320 is provided with a top film 321, the top film 321 is made of inextensible flexible materials, and the natural state of the top film 321 is hemispherical; a liquid inlet is formed at the bottom end of the inner cylinder 320, and the inner cavity of the inner cylinder 320 is communicated with the bottom end of the liquid box 102 arranged in the base platform 1 through a liquid inlet pipeline 322 and a liquid pump;

the bottom table 1 is also internally provided with a driving assembly 4 for driving the homogenizing assembly 3 to vertically slide along the guide rod 210.

The liquid inlet pipeline 322 is a flexible corrugated pipe;

the liquid in the inner cylinder 320 and the liquid box 102 is hydraulic oil;

when the device is used, primary muscle grains of livestock taken in advance are placed into the homogenate component 3, and the guide rod 210 of the upper bracket 2 is connected with the lower support rod 101 in an abutting mode. The inner cylinder 320 is filled with liquid in the initial state, the top film 321 protrudes to be hemispherical, and the homogenate component 3 moves upwards to contact with the squeezing block 203 under the action of the driving component 4, so that the muscle granules are squeezed, and the muscle granules are changed into smaller granules. During one compression, the top membrane 321 is protruded, so that the maximum pressure is applied to the muscle grains by the compression block 203.

After completion of the extrusion, a portion of the liquid is extracted from the inner cartridge 320, and the volume V of extracted liquid satisfies the equation:

wherein r is equal to the radius of the inner ring of the top end face of the inner cylinder 320;

then, the drive assembly 4 lifts the homogenate assembly 3 again, so that the squeezing block 203 is in contact with the top membrane 321, and the muscle granules are squeezed secondarily by combining the folds of the top membrane 321 from the spherical surface to the plane under the pushing force given by the drive assembly 4.

After finishing the secondary extrusion, the liquid in the inner cylinder 320 is pumped out by the volume V again, the top film 321 becomes the concave spherical surface at the moment, and the step of lifting the homogenate component 3 is repeated, thereby realizing the tertiary extrusion of muscle granules.

The process is repeated for a plurality of times, so that the muscle granules are homogenized to be changed from granular to pasty, the pasty form can be better attached to the inner wall of the culture container, and therefore inoculation can be better performed, the influence of the culture solution injection process after inoculation of the pasty form is not easy to affect, and the quality of cell culture is improved.

Example 2

On the basis of embodiment 1, the extrusion block 203 includes a spherical block 231 corresponding to the inner diameter of the top end of the inner cylinder 320. The spherical block 231 can be better attached to the concave state of the top film 321, so that a better slurry homogenizing effect is obtained.

A sealing cylinder 232 is arranged on the periphery of the spherical block 231 of the extrusion block 203, the inner diameter of the sealing cylinder 232 corresponds to the outer diameter of the outer cylinder 310, and a sealing ring is arranged at the lower end of the sealing cylinder 232;

the inner top of the closed cylinder 232 is located above the horizontal section of the spherical block 231, and the horizontal section is a horizontal section passing through the spherical center of the spherical block 231;

the horizontal plane of the bottom end of the closed cylinder 232 is located below the bottom end surface of the spherical block 231.

Through the closed cylinder 232, when the homogenate component 3 moves upwards, the closed cylinder 232 is sleeved outside the outer cylinder 310, air in the closed cylinder 232 is difficult to discharge through the sealing ring, especially, when the top membrane 321 is in a concave state, because the air is very easy to compress, and the internal air volume is small, normal extrusion homogenization cannot be influenced, and the complete attachment of the spherical block 231 and the top membrane 321 can be prevented through compressed air, so that the condition that muscle granules are excessively extruded to cause cell damage is avoided.

Example 3

On the basis of the above embodiment, the elastic pad 233 is provided at the tip of the pressing block 203.

When the device is used, in order to ensure the extrusion pressure degree of the extrusion block 203, a user presses the top of the upper bracket 2 downwards from the upper part by hand, and presses the position like the outer shell of the top motor 240, when downward pressure is applied, because the closed cylinder 232 causes the internal air of the closed cylinder to be difficult to discharge so as to generate reverse upward thrust, the elastic pad 233 can have a good buffering effect on the force, and the effect of avoiding the muscle tissue cells from being excessively extruded and damaged is also achieved through the stress contraction of the elastic pad 233.

Example 4

On the basis of the above embodiment, the top motor 240 is fixedly disposed on the upper side of the bearing plate 220, a motor shaft of the top motor 240 penetrates through the bearing plate 220 to be linked with the spherical block 231, and the motor shaft of the top motor 240 is coaxial with the spherical block 231.

The closed cylinder 232 is connected with the spherical block 231 through a bearing.

The connection mode here is that the top end of the closed cylinder 232 is fixedly connected with the lower side of the bearing plate 220, the spherical block 231 is connected with the inner wall of the closed cylinder 232 through a bearing, and the top end of the spherical block 231 is connected with the motor shaft of the top motor 240 through the elastic pad 233.

The top motor 240 drives the ball 231 to rotate or twist the ball 231, so that the ball 231 can achieve better homogenization effect when contacting with muscle granules.

Example 5

On the basis of the above embodiment, horizontal plates 311 are symmetrically arranged on both sides of the lower portion of the outer cylinder 310, and through holes are formed in the horizontal plates 311 and are slidably connected with the guide rods 210 through the through holes;

the driving assembly 4 comprises a cam 401 positioned below the horizontal plate 311, and the axle of the cam 401 is linked with the motor shaft of the lower motor 420;

a wheel axle hole 411 is formed in a wheel body of the cam 401, four outer convex surfaces located on the periphery side of the wheel axle hole 411 are arranged on the wheel body, each outer convex surface is an arc-shaped surface, and a plane is arranged between every two adjacent outer convex surfaces;

a connecting line from the circle center of the axle hole 411 to the farthest point of each outer convex surface is in a cross shape, and the length of the connecting line from the circle center of the axle hole 411 to the farthest point of each outer convex surface is sequentially increased by the length r in a clockwise or anticlockwise sequence;

r is equal to the radius of the inner ring of the top end face of the inner cylinder 320;

the length of the connecting line from the center of the axle hole 411 to the far end point of the nearest outer convex surface is equal to r.

By the structure, the device is in an initial state that the outer convex surface of the cam 401 closest to the axle hole 411 is positioned at the upper side, the bottom surface of the horizontal plate 311 is attached to the outer convex surface, the distance between the bottom end of the spherical block 231 and the top end of the top film 231 in a convex state is r, after the cam 401 rotates by 90 degrees, the horizontal plate 311 is jacked up for a moving distance r, and at the moment, the bottom end of the spherical block 231 is contacted with the top end of the top film 231 to perform primary extrusion; when the secondary extrusion is performed, the top film 231 retracts by a distance r, the cam 401 rotates by 90 degrees again, and the lifting distance of the horizontal plate 311 relative to the initial position is 2 r; by analogy, when the extrusion is performed for three times, the lifting distance of the horizontal plate 311 relative to the initial position is 3 r. The cam 401 returns after rotating for 90 degrees, and the operation is repeated.

The advantage of using this structure is that it can combine with a stepping motor as the cam 401, and the quantitative lifting of the horizontal plate 311 can be realized by rotating the cam 401 90 degrees each time. Compared with the hydraulic and pneumatic push rods and the cam for quantitative driving, the device can run more stably, and the manufacturing cost is far lower than that of the pneumatic and hydraulic driving modes. In addition, the cam is used as a lifting mechanism, and other mechanical structures which move periodically can be combined to be used as a driving structure, so that the common one-way motor can be used for driving.

Example 6

On the basis of the above embodiment, the bottom end of the inner cylinder 320 is fixedly connected with the screw rod 323, the screw rod 323 is hollow and communicated with the inside of the inner cylinder 320, the screw rod 323 is provided with a liquid inlet hole, and the bottom end of the screw rod 323 is connected with the liquid inlet pipeline 322;

the bottom end of the outer cylinder 310 is rotatably connected with an adjusting ring 312, and the inner side of the adjusting ring 312 is provided with internal threads which are in threaded connection with a screw rod 323;

the outer barrel 310 is provided with a vertical limiting groove in the lower portion, the outer lower portion of the inner barrel 320 is fixedly provided with a limiting block corresponding to the limiting groove, and the inner barrel 320 is connected with the outer barrel 310 in a sliding mode through the limiting block and the limiting groove.

Through this structure, after accomplishing the homogeneity, can be with rotating adjustable ring 312 drive lead screw 323 motion to upwards jack-up inner tube 320, make things convenient for the staff to take out the meat paste after the homogeneity.

Example 7

A method for culturing primary muscle cells of livestock comprises the steps of,

s1, obtaining muscle blocks from livestock;

s2, shearing the blocks obtained in the S1 into muscle granules;

s3, rolling the muscle granules of S2 to make the muscle granules into paste;

s4, inoculating the paste obtained in the step S3 into a culture container, and waiting until the paste is attached to the wall completely;

s5, adding a culture solution.

Example 8

A method of homogenizing primary muscle cells of a domestic animal using the tissue homogenizer of claim 1, comprising the steps of,

a1, putting the pre-taken primary livestock muscle grains into the homogenate component 3, and pumping liquid into the inner cylinder 320 in the initial state to make the top membrane 321 protrude into a semi-sphere shape;

a2, making the homogenate component 3 move upwards to contact with the squeezing block 203 to form primary squeezing on the muscle particles;

a3, extracting a part of the liquid in the inner cylinder 320, wherein the volume V of the extracted liquid conforms to the formula:

wherein r is equal to the radius of the inner ring of the top end face of the inner cylinder 320;

a4, lifting the homogenate component 3 again to enable the extrusion block 203 to contact with the top membrane 321, and carrying out secondary extrusion on muscle granules;

a5, repeating the steps A3 and A4, and extruding for three times;

a6, repeating the steps A2 to A5 for a plurality of times in a circulating mode until the homogenization is finished.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (10)

1. The tissue homogenizer for primary muscle cell culture of the livestock is characterized by comprising a base platform (1), wherein the base platform (1) comprises a plurality of lower supporting rods (101) which are vertically arranged, the base platform (1) is connected with an upper support (2) through the lower supporting rods (101), the upper support (2) comprises a plurality of guide rods (210) corresponding to the lower supporting rods (101), and the bottom ends of the guide rods (210) are inserted into the top ends of the lower supporting rods (101); the top end of the guide rod (210) is provided with a horizontal bearing plate (220), and the lower side of the bearing plate (220) is provided with an extrusion block (203);

the guide rod (210) is connected with a homogenizing assembly (3) in a sliding mode, and the homogenizing assembly (3) comprises an outer cylinder (310) and an inner cylinder (320) which are sleeved; the top end of the outer cylinder (310) is open, the top end of the inner cylinder (320) is provided with a top film (321), the top film (321) is made of inextensible flexible materials, and the natural state of the top film (321) is a semi-spherical surface; the bottom end of the inner cylinder (320) is provided with a liquid inlet, and the inner cavity of the inner cylinder (320) is communicated with the interior of a liquid box (102) arranged in the base platform (1) through a liquid inlet pipeline (322) and a liquid pump;

and a driving assembly (4) for driving the homogenizing assembly (3) to vertically slide along the guide rod (210) is further arranged in the bottom table (1).

2. The tissue homogenizer of claim 1, wherein the expression block (203) comprises a spherical block (231) corresponding to the inner diameter of the top end of the inner cylinder (320).

3. The tissue homogenizer according to claim 2, wherein a closed cylinder (232) is arranged around the spherical block (231) of the pressing block (203), the inner diameter of the closed cylinder (232) corresponds to the outer diameter of the outer cylinder (310), and a sealing ring is arranged at the lower end of the closed cylinder (232);

the inner top of the closed cylinder (232) is positioned above the horizontal section of the spherical block (231), and the horizontal section is a horizontal section passing through the spherical center of the spherical block (231);

the horizontal plane of the bottom end of the closed cylinder (232) is positioned below the bottom end surface of the spherical block (231).

4. The tissue homogenizer of claim 1, wherein a top motor (240) is fixedly disposed on the upper side of the carrying plate (220), and a motor shaft of the top motor (240) penetrates the carrying plate (220) to be interlocked with the spherical block (231).

5. Tissue homogenizer according to claims 1-4, characterized in that the closing cylinder (232) and the spherical block (231) are connected by means of bearings.

6. The tissue homogenizer according to claims 1 to 5, wherein horizontal plates (311) are symmetrically arranged on both sides of the lower portion of the outer cylinder (310), and through holes are formed in the horizontal plates (311) and slidably connected with the guide rods (210) through the through holes.

7. The tissue homogenizer of claim 6, wherein the drive assembly (4) comprises a cam (401) located below the horizontal plate (311), the axle of the cam (401) being in communication with the motor shaft of a lower motor (420);

a wheel axle hole (411) is formed in a wheel body of the cam (401), four outer convex surfaces located on the peripheral side of the wheel axle hole (411) are arranged on the wheel body, each outer convex surface is an arc-shaped surface, and a plane is arranged between every two adjacent outer convex surfaces;

a connecting line from the circle center of the axle hole (411) to the farthest point of each outer convex surface is in a cross shape, and the length of the connecting line from the circle center of the axle hole (411) to the farthest point of each outer convex surface is sequentially increased by a length r in a clockwise or anticlockwise sequence;

r is equal to the radius of the inner ring of the top end face of the inner cylinder (320);

the length of a connecting line from the circle center of the axle hole (411) to the far end point of the nearest outer convex surface is equal to r.

8. The tissue homogenizer according to claim 1, wherein the bottom end of the inner cylinder (320) is fixedly connected with a screw rod (323), the screw rod (323) is hollow and is communicated with the interior of the inner cylinder (320), the screw rod (323) is provided with the liquid inlet hole, and the bottom end of the screw rod (323) is connected with the liquid inlet pipeline (322);

the bottom end of the outer barrel (310) is rotatably connected with an adjusting ring (312), and the inner side of the adjusting ring (312) is provided with internal threads which are in threaded connection with the screw rod (323);

the outer barrel (310) lower part is provided with a vertical limiting groove, the outer lower part of the inner barrel (320) is fixedly provided with a limiting block corresponding to the limiting groove, and the inner barrel (320) is connected with the outer barrel (310) in a sliding mode through the limiting block and the limiting groove.

9. A method for culturing primary muscle cells of livestock is characterized by comprising the following steps,

s1, obtaining muscle blocks from livestock;

s2, shearing the blocks obtained in the S1 into muscle grains;

s3, rolling the muscle granules of S2 to make the muscle granules into paste;

s4, inoculating the paste obtained in the step S3 into a culture container, and waiting until the paste is attached to the wall completely;

s5, adding a culture solution.

10. A method for homogenizing primary muscle cells of livestock, wherein the tissue homogenizer of claim 1 is used, comprising the steps of:

a1, putting the primary muscle grains of the livestock taken in advance into a homogenate component (3), pumping liquid into the inner cylinder (320) in the initial state, and enabling the top membrane (321) to be protruded to be hemispherical;

a2, making the homogenate component (3) move upwards to contact with the squeezing block (203) to form primary squeezing on muscle granules;

a3, extracting a part of the liquid in the inner cylinder (320), wherein the volume V of the extracted liquid conforms to the formula:

wherein r is equal to the radius of the inner ring of the top end face of the inner cylinder (320);

a4, lifting the homogenate component (3) again to enable the extrusion block (203) to be in contact with the apical membrane (321) for secondary extrusion of muscle granules;

a5, repeating the steps A3 and A4, and extruding for three times;

a6, repeating the steps A2 to A5 for a plurality of times in a circulating mode until the homogenization is finished.

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