CN206486530U - A kind of histocyte extracts and uses cryogrinding box - Google Patents
A kind of histocyte extracts and uses cryogrinding box Download PDFInfo
- Publication number
- CN206486530U CN206486530U CN201720174806.1U CN201720174806U CN206486530U CN 206486530 U CN206486530 U CN 206486530U CN 201720174806 U CN201720174806 U CN 201720174806U CN 206486530 U CN206486530 U CN 206486530U
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- cylinder
- box
- fixedly mounted
- mortar
- electric expansion
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Abstract
The utility model discloses a kind of histocyte extraction cryogrinding box, box body including upper end open, four cylinders, electric machine controller, electric expansion rod controller and cross bar, four cylinders are vertically installed in tray interior, it is right against on the bottom plate of box body lower end at cylinder and is equipped with a through hole, through hole is right against on box bottom an electric expansion bar is fixedly mounted, the telescopic end of electric expansion bar is both passed through in the described cylinder of corresponding through hole insertion, and a block rubber is fixedly mounted, block rubber surface is provided with a hemispheric standing groove, the mortar of a hemispherical dome structure is equipped with standing groove, cross bar is arranged at cassette upper end, it is right against on cross bar bottom surface at cylinder and a motor is fixedly mounted, the rotating shaft of motor all extends to corresponding inner barrel, and a spheroid is fixedly mounted.The utility model is simple in construction, effectively a small amount of or micro tissue can be ground, also allow for collecting and shift, it is to avoid the experimental result of influence.
Description
Technical field
The utility model is related to technical field of experiment equipment, and in particular to a kind of histocyte extracts and uses cryogrinding box.
Background technology
Animal vegetable tissue cell extraction process is the important step of molecular biology experiment, the nucleus that the step is extracted
Quality can directly have influence on the experimental result in downstream, traditional extracting mode is to heat liquid nitrogen in mortar quickly to grind
Disrupting tissue, afterwards, takes the powder organization after some grindings to add in FP pipes, still, such mode is only applicable to extraction group
Knit more sample, the mode for some a small amount of or micro tissue above is not just easy to operate, be both not easy to grinding and also not
It is easy to the collection transfer organized after grinding, the result for causing and testing that downstream can be influenceed to test.
Utility model content
To solve the above problems, the utility model is provided, one kind is simple in construction, and experiment is convenient, can be effectively to micro
Tissue is ground, also allow for collecting and shift, it is to avoid the histocyte of influence experimental result, which is extracted, uses cryogrinding box.
To achieve the above object, the technical scheme that the utility model is taken is：
A kind of histocyte, which is extracted, uses cryogrinding box, including the box body of upper end open, four cylinders, electric machine controller,
Electric expansion rod controller and cross bar, four cylinders are vertically installed in tray interior, and cylinder is communicated up and down, the bottom of box body lower end
It is right against on plate at cylinder and is equipped with a through hole, through hole is right against on box bottom an electric expansion bar is fixedly mounted, electricity
The telescopic end of dynamic expansion link is both passed through in the described cylinder of corresponding through hole insertion, and a block rubber, block rubber is fixedly mounted
Internal face of the outer ring face with cylinder offset, block rubber surface is provided with a hemispheric standing groove, places in groove bottom
Provided with more than one stopper slot, the mortar of a hemispherical dome structure is equipped with standing groove, mortar is right against stopper slot on bottom surface
Place is equipped with a limited block, and limited block is inserted in corresponding stopper slot, vertical on the outer wall on the right side of box body to be fixedly mounted one
Be slidably installed a sliding block on cylindrical guide, cylindrical guide, and cross bar is fixedly installed in shoe surface, between cross bar and four cylinders
It is oppositely arranged, is right against on cross bar bottom surface at cylinder and a motor is fixedly mounted, a straight-bar is fixedly mounted in the rotating shaft of motor,
Straight-bar one end all extends to corresponding inner barrel, and a spheroid is fixedly mounted.
As preferred technical scheme, mortar is made by transparent aseptic plastic, and the diameter and FP pipes of mortar opening are interior
The diameter in portion is identical, and the size of spheroid is identical with the size inside mortar, can offset between the two.
As preferred technical scheme, it is fixedly connected between cylinder and cylinder by connecting rod, the cylinder positioned at both sides
Also it is fixedly connected, is also fixedly mounted on box bottom under two support bars, two support bars with the inwall of box body by connecting rod
A bottom plate is fixedly mounted in end.
As preferred technical scheme, electric machine controller is fixedly installed on cylindrical guide right flank, electric machine controller with
Connected between four motors by electric wire, electric machine controller is also fixedly connected by electric wire with extraneous power supply, electric expansion bar control
Device processed is fixedly installed on bottom plate, is connected between electric expansion rod controller and four electric expansion bars by electric wire, electronic to stretch
Contracting rod controller is also fixedly connected by electric wire with extraneous power supply.
The utility model has the advantages that：The utility model structure cylinder is single, and easy to use, financial cost is low, makes
It is with scope wide, effectively a small amount of or micro tissue can be ground, also allow for collecting and shift, it is to avoid the reality influenceed
Test result.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the utility model embodiment；
Fig. 2 is the partial enlarged drawing at A in Fig. 1.
Embodiment
In order that the purpose of this utility model and advantage are more clearly understood, the utility model is entered with reference to embodiments
Row is further described.It should be appreciated that specific embodiment described herein is only to explain the utility model, and without
In restriction the utility model.
As depicted in figs. 1 and 2, the utility model embodiment provides a kind of extraction of histocyte and uses cryogrinding box, wraps
Include box body 3, four cylinders 4, electric machine controller 12, electric expansion rod controller 14 and the cross bar 8 of upper end open, four cylinders 4
Be vertically installed in inside box body 3, cylinder 4 up and down communicate, be right against on the bottom plate of the lower end of box body 3 at cylinder 4 be equipped with one lead to
It is right against on hole 2, the bottom surface of box body 3 at through hole 2 and an electric expansion bar 1 is fixedly mounted, the telescopic end of electric expansion bar 1 is both passed through
Corresponding through hole 2 is inserted in described cylinder 4 (gap between expansion link and through hole passes through seal with elastometic washer), and fixed peace
Fill a block rubber 9, the internal face of the outer ring face of block rubber 9 with cylinder 4 offsets, and the surface of block rubber 9 is hemispheric provided with one
It is equipped with standing groove (unmarked), placement groove bottom in more than one stopper slot 17, standing groove and is equipped with a hemispherical junction
It is right against on the mortar 19 of structure, the bottom surface of mortar 19 at stopper slot 17 and is equipped with a limited block 18, limited block 18 inserts corresponding
Stopper slot 17 in, by limited block effectively can position mortar, it is to avoid the rotation of mortar, on the outer wall on the right side of box body 3
Vertical that the sliding block 20 that is slidably installed is fixedly mounted on a cylindrical guide 11, cylindrical guide 11, cross bar 8 is fixedly installed in the table of sliding block 20
On face, it is oppositely arranged between cross bar 8 and four cylinders 4, is right against on the bottom surface of cross bar 8 at cylinder 4 and a motor 7 is fixedly mounted,
A straight-bar 6 is fixedly mounted in the rotating shaft of motor 7, the one end of straight-bar 6 is all extended to inside corresponding cylinder 4, and is fixedly mounted
One spheroid 5.
In the present embodiment, mortar 19 is made by transparent aseptic plastic, inside the diameter and EP pipes that mortar 19 is open
Diameter is identical so that mortar can be just placed into EP pipes, and enables the face between mortar and EP pipes by transparent mortar
Color is identical, facilitates the operation tested to downstream, and the size of spheroid 5 is identical with the size inside mortar 19, between the two can phase
Support.
In the present embodiment, it is fixedly connected between cylinder and cylinder by connecting rod, the cylinder positioned at both sides is also by even
Extension bar is fixedly connected with the inwall of box body 3, and two support bars 15, two lower ends of support bar 15 are also fixedly mounted on the bottom surface of box body 3
A bottom plate 16 is fixedly mounted.
In the present embodiment, electric machine controller 12 is fixedly installed on the right flank of cylindrical guide 11, electric machine controller 12 and four
Connected between individual motor 7 by electric wire, electric machine controller 12 is also fixedly connected by electric wire with extraneous power supply, electric expansion bar control
Device 14 processed is fixedly installed on bottom plate 16, is connected between electric expansion rod controller 14 and four electric expansion bars 1 by electric wire,
Electric expansion rod controller 14 is also fixedly connected by electric wire with extraneous power supply.
Operation principle：A certain amount of liquid nitrogen is poured into toward box body, liquid nitrogen need to flood the general of cylinder, after the completion of, first upwards
Mobile cross bar, makes spheroid be removed from cylinder, after removal, starts electric expansion bar by electric expansion rod controller, makes electronic
The telescopic end of expansion link stretches out, so that at mortar top to the opening of cylinder upper end by block rubber and on block rubber, it is past to grind
A small amount of tissue is put into alms bowl and a liquid nitrogen is added, after the completion of, the flexible of electric expansion bar is made by electric expansion rod controller
End is retracted, and block rubber is moved to the middle position of cylinder, because cylinder is submerged in liquid oxygen so as to move to the tissue of middle
Low-temperature condition can be effectively kept, after the completion of, cross bar is moved down, spheroid is inserted in corresponding cylinder, and pass through motor control
Device starts motor, effectively spheroid can be driven to rotate by motor, during rotation, by cross bar again slowly to moving down
Dynamic ball-type, the tissue in mortar until making spheroid extend in corresponding mortar, and rolled and ground by the spheroid of rotation
Mill, and the size due to spheroid is identical with the size inside mortar so that spheroid can be contacted preferably with the internal face of mortar, from
And add the effect of grinding, after the completion of, again spheroid is removed by cylinder by cross bar, and again pass through electric expansion bar
At mortar top to cartridge openings, specifically, directly mortar can be taken down from block rubber, and directly put it into EP pipes
In, facilitate the collection transfer to tissue.
This specific implementation is simple in construction, easy to use, and financial cost is low, wide using scope, can effectively on a small quantity or
Micro tissue is ground, also allow for collecting and shift, it is to avoid the experimental result of influence.
Described above is only preferred embodiment of the present utility model, it is noted that for the common skill of the art
For art personnel, on the premise of the utility model principle is not departed from, some improvements and modifications can also be made, these improve and
Retouching also should be regarded as protection domain of the present utility model.
Claims (4)
1. a kind of histocyte extracts and uses cryogrinding box, it is characterised in that：Box body including upper end open, four cylinders, electricity
Machine controller, electric expansion rod controller and cross bar, four cylinders are vertically installed in tray interior, and cylinder is communicated up and down, box
It is right against on the bottom plate of body lower end at cylinder and is equipped with a through hole, through hole is right against on box bottom, and to be fixedly mounted one electronic
Expansion link, the telescopic end of electric expansion bar is both passed through in the described cylinder of corresponding through hole insertion, and a rubber is fixedly mounted
Block, the internal face of the outer ring face of block rubber with cylinder offsets, and block rubber surface is provided with a hemispheric standing groove, standing groove
It is just right on the mortar that a hemispherical dome structure is equipped with more than one stopper slot, standing groove, mortar bottom surface to be equipped with bottom surface
A limited block is equipped with stopper slot, limited block is inserted in corresponding stopper slot, it is vertically solid on the outer wall on the right side of box body
Dingan County fills the sliding block that is slidably installed on a cylindrical guide, cylindrical guide, and cross bar is fixedly installed in shoe surface, cross bar and four
It is oppositely arranged between cylinder, is right against on cross bar bottom surface at cylinder and a motor is fixedly mounted, fixed peace in the rotating shaft of motor
A straight-bar is filled, straight-bar one end all extends to corresponding inner barrel, and a spheroid is fixedly mounted.
2. histocyte according to claim 1 extracts and uses cryogrinding box, it is characterised in that：Mortar is by transparent nothing
Bacterium plastics are made, and the diameter of mortar opening is identical with the diameter inside FP pipes, and the size of spheroid is identical with the size inside mortar,
It can offset between the two.
3. histocyte according to claim 1 extracts and uses cryogrinding box, it is characterised in that：Between cylinder and cylinder
It is fixedly connected by connecting rod, the cylinder positioned at both sides is also fixedly connected by connecting rod with the inwall of box body, on box bottom
Two support bars are also fixedly mounted, a bottom plate is fixedly mounted in two support bar lower ends.
4. histocyte according to claim 1 extracts and uses cryogrinding box, it is characterised in that：Electric machine controller fixes peace
Loaded on cylindrical guide right flank, connected between electric machine controller and four motors by electric wire, electric machine controller also passes through electricity
Line is fixedly connected with extraneous power supply, and electric expansion rod controller is fixedly installed on bottom plate, electric expansion rod controller and four
Connected between electric expansion bar by electric wire, electric expansion rod controller is also fixedly connected by electric wire with extraneous power supply.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720174806.1U CN206486530U (en) | 2017-02-24 | 2017-02-24 | A kind of histocyte extracts and uses cryogrinding box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720174806.1U CN206486530U (en) | 2017-02-24 | 2017-02-24 | A kind of histocyte extracts and uses cryogrinding box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206486530U true CN206486530U (en) | 2017-09-12 |
Family
ID=59762438
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720174806.1U Expired - Fee Related CN206486530U (en) | 2017-02-24 | 2017-02-24 | A kind of histocyte extracts and uses cryogrinding box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206486530U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110575895A (en) * | 2019-10-20 | 2019-12-17 | 连云港睿晶石英材料有限公司 | Sample grinding equipment for quartz material detection |
| CN112574868A (en) * | 2020-12-15 | 2021-03-30 | 山东省农业科学院畜牧兽医研究所 | A tissue homogenizer for primary muscle cell culture of domestic animal |
-
2017
- 2017-02-24 CN CN201720174806.1U patent/CN206486530U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110575895A (en) * | 2019-10-20 | 2019-12-17 | 连云港睿晶石英材料有限公司 | Sample grinding equipment for quartz material detection |
| CN112574868A (en) * | 2020-12-15 | 2021-03-30 | 山东省农业科学院畜牧兽医研究所 | A tissue homogenizer for primary muscle cell culture of domestic animal |
| CN112574868B (en) * | 2020-12-15 | 2022-08-02 | 山东省农业科学院畜牧兽医研究所 | A tissue homogenizer for primary muscle cell culture of domestic animal |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170912 Termination date: 20180224 |