CN115069316B - Chip capable of detecting multiple body fluids simultaneously - Google Patents
Chip capable of detecting multiple body fluids simultaneously Download PDFInfo
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- CN115069316B CN115069316B CN202210703393.7A CN202210703393A CN115069316B CN 115069316 B CN115069316 B CN 115069316B CN 202210703393 A CN202210703393 A CN 202210703393A CN 115069316 B CN115069316 B CN 115069316B
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- injection
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- 210000001124 body fluid Anatomy 0.000 title claims abstract description 64
- 239000010839 body fluid Substances 0.000 title claims abstract description 64
- 239000007788 liquid Substances 0.000 claims abstract description 400
- 238000002347 injection Methods 0.000 claims abstract description 158
- 239000007924 injection Substances 0.000 claims abstract description 158
- 238000001514 detection method Methods 0.000 claims abstract description 50
- 238000009434 installation Methods 0.000 claims abstract description 30
- 230000000712 assembly Effects 0.000 claims abstract 3
- 238000000429 assembly Methods 0.000 claims abstract 3
- 239000012295 chemical reaction liquid Substances 0.000 claims description 112
- 239000002699 waste material Substances 0.000 claims description 31
- 238000007789 sealing Methods 0.000 claims description 22
- 238000005086 pumping Methods 0.000 claims description 20
- 230000005540 biological transmission Effects 0.000 claims description 13
- 239000006185 dispersion Substances 0.000 claims description 13
- 230000005389 magnetism Effects 0.000 claims description 3
- 239000002184 metal Substances 0.000 description 19
- 210000003296 saliva Anatomy 0.000 description 18
- 239000000758 substrate Substances 0.000 description 7
- 238000000605 extraction Methods 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000004062 sedimentation Methods 0.000 description 5
- 239000003599 detergent Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 102000004338 Transferrin Human genes 0.000 description 3
- 108090000901 Transferrin Proteins 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000012581 transferrin Substances 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 210000001138 tear Anatomy 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
Abstract
The invention discloses a chip capable of detecting various body fluids simultaneously, which comprises a chip assembly, a plurality of groups of liquid inlet assemblies and a first reaction assembly, wherein the chip assembly is provided with a plurality of liquid inlet channels, and the chip assembly is provided with a plurality of mounting grooves; the liquid inlet component is arranged in the corresponding mounting groove; the first reaction component comprises a centralized injection seat, wherein a plurality of centralized injection ports communicated with the liquid inlet channels are arranged at one end of the centralized injection seat, the other end of the centralized injection seat is connected with a dispersed injection seat, a plurality of mutually independent liquid outlet grooves are formed at one end of the dispersed injection seat, a plurality of liquid outlet holes which correspond to the liquid outlet grooves one by one are arranged at the other end of the dispersed injection seat, a rotating ring is rotatably connected in a chip component at the other end of the first installation channel, a guide shaft is fixed in the chip component, and a reaction ring which is controlled by magnetic force and can be inserted on the rotating ring is slidably connected on the guide shaft; the invention can realize the simultaneous detection of various body fluids.
Description
Technical Field
The invention relates to the technical field of cancer in-vitro detection, in particular to a chip capable of detecting various body fluids simultaneously.
Background
The microfluidic technology is used for detecting body fluids such as saliva, urine, tears and the like, the sampling amount is small, and the microfluidic device is suitable for on-site detection.
In the prior art, the name of the microfluidic saliva detection chip is disclosed, the publication number is CN 215996712U, the publication date is 2022.03.11 of Chinese patent utility model, the microfluidic saliva detection chip comprises an upper substrate and a lower substrate, the lower substrate is fixedly arranged at the bottom of the upper substrate, a sliding type filtering mechanism is detachably arranged at the left side of the upper substrate, a carrier plate in the sliding type filtering mechanism is arranged on the inner side of a containing groove in the sliding type filtering mechanism in a sliding type manner, saliva detection can be conveniently realized, simultaneous detection of various body fluids cannot be realized, and the function is single.
Disclosure of Invention
This section is intended to outline some aspects of embodiments of the utility model and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description summary and in the title of the application, to avoid obscuring the purpose of this section, the description summary and the title of the utility model, which should not be used to limit the scope of the utility model.
The present utility model has been made in view of the above and/or existing problems in body fluid testing.
It is therefore an object of the present invention to provide a chip capable of detecting a plurality of body fluids simultaneously, which enables simultaneous detection of a plurality of body fluids.
In order to solve the technical problems, the invention provides the following technical scheme: a chip capable of detecting a plurality of body fluids simultaneously, comprising,
the chip assembly is provided with a first detection groove at one end, a first installation channel is arranged in the chip assembly, a plurality of liquid inlet channels are arranged on the chip assembly at one end of the first installation channel, and a plurality of installation grooves are arranged on the chip assembly;
the liquid inlet components are provided with a plurality of groups and are in one-to-one correspondence with the mounting grooves, and the liquid inlet components are mounted in the corresponding mounting grooves;
the first reaction subassembly, first reaction subassembly is including fixing in the chip subassembly and installing the concentrated injection seat at first mounting channel one end, concentrated injection seat's one end has been arranged a plurality of and the concentrated filling mouth of feed liquor passageway intercommunication, concentrated injection seat's the other end is connected with the dispersion and has injected the seat, the dispersion is injected the seat and is concentrated the one end of injection seat relatively and open has first play cistern, second play cistern, third play cistern and fourth play cistern of mutual independence, and the one end that concentrated injection seat was kept away from to the dispersion is arranged a plurality of and first play liquid groove intercommunication's first liquid outlet, the second liquid outlet of a plurality of and first liquid outlet groove intercommunication, a third liquid outlet and a fourth liquid outlet of a and fourth liquid outlet groove intercommunication, rotationally be connected with the swivel ring in the chip subassembly of first mounting channel other end, the one end fixedly connected with guiding axle that concentrated injection seat was kept away from to the dispersion injection seat, the one end fixedly connected with of dispersion injection seat is in the chip subassembly, can be controlled to slide on the guiding axle and have the first magnetic force control on the one-to-one side is connected with the magnetic force injection ring, the first fluid injection ring is arranged in the first magnetic force injection ring, the first magnetic force injection ring is arranged in the first magnetic force injection groove is connected with the first magnetic force injection groove, the first magnetic force injection ring is arranged in the first magnetic force injection groove, the first magnetic force injection reaction ring has the magnetic force injection device, and the magnetic force injection device is detected and has the magnetic force.
The section of the chip assembly among the reaction ring, the reaction ring and the detection groove is transparent; in the initial state, the reaction ring is closely attached to one side of the dispersion injection seat far away from the centralized injection seat, four liquid inlet channels are arranged, different body fluids are introduced into the corresponding liquid inlet channels through each liquid inlet component, the different body fluids respectively enter each centralized injection opening through the corresponding liquid inlet channels, the body fluids flowing out of the four centralized injection openings respectively enter a first liquid outlet groove, a second liquid outlet groove, a third liquid outlet groove and a fourth liquid outlet groove, the body fluids in the first liquid outlet groove are separated into a plurality of paths to be output from a plurality of first liquid outlets, the body fluids respectively enter the injection pipes through the corresponding first inflow injection openings, the body fluids in the second liquid outlet groove sequentially enter the corresponding injection pipes through the second liquid outlet openings and the corresponding first inflow injection openings, the body fluids in the third liquid outlet groove sequentially enter the corresponding injection pipes through the third liquid outlet openings and the corresponding first inflow openings, the body fluid in the fourth liquid outlet tank sequentially enters the corresponding injection pipe through the fourth liquid outlet and the corresponding first inflow injection opening, the liquid inlet is ended, the first electromagnet is electrified, the strength of the first electromagnet is regulated, the injection pipe is sucked into the corresponding reaction liquid storage tank, the reaction ring is not moved, the outer end of the injection pipe just seals the reaction ring, the body fluid reacts with the reaction liquid in the reaction liquid storage tank, the strength of the first electromagnet is increased, the reaction ring slides along the guide shaft towards the direction of the rotating ring until the reaction ring slides onto the rotating ring, the rotating ring rotates, after the body fluid and the reaction liquid are fully mixed, the rotating ring stops rotating, a color sensor can be arranged in the detection tank during detection, the rotating ring rotates, the reaction liquid storage tank is aligned with the color sensor, and the color change after the reaction is identified for analysis; according to the invention, through the combined arrangement of the first reaction component and the chip component, the simultaneous detection of various body fluids is realized, and the detection efficiency is improved.
As a preferable mode of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: the reaction ring on one side of the reaction liquid storage pool, which is opposite to the injection pipe, is provided with a sealing opening, an injection cavity is formed between the sealing opening and the inner wall of one end of the reaction ring, which is far away from the reaction liquid storage pool, the end of the injection pipe, which is far away from the dispersion injection seat, is provided with a sealing part, the sealing part can just plug the sealing opening, and when a first inflow injection opening of the injection pipe is in a corresponding first liquid outlet, a second inflow injection opening is in the injection cavity.
As a preferable mode of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: the liquid inlet assembly comprises a liquid inlet shell which is connected in a corresponding installation sinking groove in a lifting manner and is provided with an upward opening, a pressing plate is connected in the liquid inlet shell in a sliding manner, a center of the liquid inlet shell is fixedly connected with a center shaft, one end of the center shaft extending out of the liquid inlet shell in the axial direction is fixedly connected with a fixing ring, at least two linear drivers are arranged on the fixing ring, a telescopic rod capable of performing reciprocating linear movement in the height direction is connected to the linear drivers, the lower end of the telescopic rod is connected to the upper side of the pressing plate, an upward liquid inlet counter bore is formed in the center shaft, a liquid inlet is formed in the side part of the center shaft below the pressing plate, and a liquid inlet cavity is formed between the lower side of the pressing plate and the inner wall of the liquid inlet shell.
As a preferable mode of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: still include the second reaction subassembly, still be equipped with the second installation passageway in the chip subassembly, the second reaction subassembly is including connecting first storage shell and a plurality of second storage shell in the second installation passageway, be equipped with the reaction drain flow channel of vertical setting in the chip subassembly, the inner chamber of first storage shell can with the reaction drain flow channel intercommunication, the inner chamber of second storage shell can with the reaction drain flow channel intercommunication, the inner chamber of first storage shell can with one of them feed liquor chamber intercommunication in the feed liquor subassembly, still be equipped with a plurality of groups end to end's reaction unit in the chip subassembly, the reaction unit includes first reaction channel, first reaction channel one end and the reaction drain flow channel intercommunication in the reaction unit of head end, the one end of second reaction channel is connected to the first reaction channel other end, the other end and the third reaction channel one end intercommunication of second reaction channel, in the two adjacent reaction units, the preceding third reaction channel other end and the one end intercommunication of next first reaction channel.
As a preferable mode of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: the chip assembly is characterized in that the reaction liquid control motor is fixedly connected with the reaction liquid control motor, the reaction liquid control motor is connected with the liquid injection pipe which extends downwards, one side of the first storage shell, which is arranged relative to the liquid injection pipe, is provided with the first liquid inlet, one side of the second storage shell, which is arranged relative to the liquid injection pipe, is provided with the second liquid inlet, one end of the liquid injection pipe can be attached to the outer side of the first liquid inlet and aligned with the first liquid inlet or attached to the outer side of the second liquid inlet and aligned with the second liquid inlet, the other end of the liquid injection pipe is fixedly connected with the first liquid extraction pump, and the lower end of the liquid injection pipe is connected with the upper end of the reaction liquid outlet runner.
As a preferable mode of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: the first reaction channel and the second reaction channel are arranged in parallel, a plurality of reaction tanks are arranged in the chip assembly between the first reaction channel and the second reaction channel, and the chip assembly of the first reaction channel and the third reaction channel facing one side of the reaction tanks is provided with a reaction liquid drain hole.
As a preferable mode of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: the chip assembly at the other end of the third reaction channel in the terminal reaction unit is internally provided with a waste liquid outlet channel, the chip assembly is internally provided with a waste liquid pool, and one end of the waste liquid outlet channel, which is far away from the terminal third reaction channel, is connected with the waste liquid pool.
As a preferable mode of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: and a second liquid pump is connected in the chip assembly at one end of the waste liquid pool far away from the reaction unit.
As a preferable mode of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: the chip assembly comprises a first chip body and a second chip body which are sequentially connected together from bottom to top, wherein one side of the first chip body facing upwards is provided with an installation sinking groove, the second chip body is provided with an installation opening corresponding to the installation sinking groove, the installation sinking groove and the installation opening form a first installation channel, the third chip body seals the first installation channel, one side of the first chip body facing upwards is provided with a plurality of groups of lower reaction units connected end to end, the lower reaction units comprise a first lower reaction liquid runner, a second lower reaction liquid runner and a third lower reaction liquid runner which are sequentially connected together, one end of the second chip body facing downwards is provided with a plurality of groups of upper reaction units connected end to end, the upper reaction units comprise an upper reaction liquid runner, a second upper reaction liquid runner and a third lower reaction liquid runner which are sequentially connected between the first lower reaction liquid runner and a corresponding first upper reaction liquid runner, an upper reaction channel with a lower reaction end which is sealed, and a lower reaction liquid runner which is formed between the second lower reaction liquid runner and a corresponding second upper reaction liquid runner, and a third reaction channel which is sealed between the second lower reaction liquid runner and a third reaction channel which is formed between the second upper reaction liquid runner and the corresponding second upper reaction liquid runner.
As a preferable mode of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: the first installation channel of the chip assembly is internally and fixedly connected with a transmission motor, and the transmission motor is connected with the rotating ring through a transmission belt.
The invention has the beneficial effects that: according to the invention, through the combined arrangement of the first reaction component and the chip component, the simultaneous detection of various body fluids is realized, and the detection efficiency is improved; through the arrangement of the second reaction component and the liquid inlet component, detection of different proteins in saliva is realized.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:
fig. 1 is a front view of the present invention.
Fig. 2 is a diagram of an exploded view of the first embodiment of the present invention.
Fig. 3 is a partial enlarged view at a in fig. 2.
Fig. 4 is a first perspective view of the third chip body, the fourth chip body and the second reaction module of the present invention.
Fig. 5 is a perspective view of a centralized injection seat according to the present invention.
Fig. 6 is a perspective view of a dispersion injecting seat according to the present invention.
Fig. 7 is a perspective view of a liquid inlet assembly according to the present invention.
Fig. 8 is a partial enlarged view at B in fig. 7.
Fig. 9 is a first perspective view of the structure of the present invention after the fifth chip body is hidden.
Fig. 10 is a partial enlarged view at C in fig. 9.
FIG. 11 is a perspective view showing a reaction ring closely attached to a dispersion injection seat according to the present invention.
FIG. 12 is a structural view of a reaction ring in the present invention.
Fig. 13 is a view from the direction H-H of fig. 12.
Fig. 14 is a diagram showing a second exploded construction of the present invention.
Fig. 15 is a partial enlarged view at D in fig. 14.
Fig. 16 is a partial enlarged view at E in fig. 14.
Fig. 17 is a second perspective view of the present invention after the fifth chip body is hidden.
Fig. 18 is a partial enlarged view of F in fig. 17.
FIG. 19 is a perspective view showing a first reaction module connected to a first chip body according to the present invention.
Fig. 20 is a partial enlarged view at G in fig. 19.
Fig. 21 is a perspective view of a fifth chip body according to the present invention.
In the figure, a 100 chip assembly, a 101 fifth chip body, a 101a reaction liquid inlet hole, a 101b liquid outlet part, a 101c first upper liquid outlet channel, a 102 fourth chip body, a 102a first vertical through hole, a 102b first lower liquid outlet channel, a 102c lower liquid outlet channel, a 103 third chip body, a 103a second vertical through hole, a 103b first detection settling tank, a 104 second chip body, a 104a third vertical through hole, a 104b upper reaction unit, a 104b-1 second upper reaction liquid channel, a 104b-2 first upper reaction liquid channel, a 104b-3 third upper reaction liquid channel, a 104c mounting port, a 104d upper liquid outlet channel, a 105 first chip body, a 105a reaction liquid inlet settling tank, a 105b lower reaction unit, a 105b-1 third lower reaction liquid channel, a 105b-2 second lower reaction liquid channel, a 105b-3 first lower reaction liquid outlet channel, a 105b-4 reaction liquid outlet hole, a 105b-5 reaction tank, a 105b-6 detection chamber, 105c lifting hole, 105d fourth liquid inlet channel, 105e second liquid inlet channel, 105f first liquid inlet channel, 105g third liquid inlet channel, 105h mounting sinking groove, 106 first detecting groove, 107 mounting groove, 107a fourth connecting port, 107b third connecting port, 107c second connecting port, 107d first connecting port, 107e connecting sinking groove, 200 injection needle, 300 liquid inlet component, 301 fourth liquid inlet component, 301a liquid inlet housing, 301a-1 liquid outlet hole, 301a-2 liquid draining part, 301b fixed ring, 301c center shaft, 301c-1 liquid inlet, 301d linear driver, 301e guide bar, 301f guide sleeve, 301g fixed outer ring, 302 third liquid inlet component, 303 second liquid inlet component, 304 first liquid inlet component, 400 first reaction component, 401 concentrated injection seat, 401a concentrated injection port, 401b first liquid outlet groove, 401c second liquid outlet groove, 401d third liquid outlet groove, 401e fourth liquid outlet groove, 402a dispersion injection seat, 402b first liquid outlet, 402c second liquid outlet, 402d second liquid inlet, 402e third liquid outlet, 402f third liquid inlet, 402g third liquid outlet, 402h fourth liquid inlet, 403 reaction ring, 403a injection cavity, 403b closing port, 403c reaction liquid storage pool, 404 guide shaft, 405 transmission motor, 406 rotating ring, 407 transmission belt, 408 first mounting channel, 409 first electromagnet, 410 injection pipe, 410a first inflow injection port, 410b second inflow injection port, 410c closing portion, 411 sealing plug, 500 second reaction component, 501 reaction liquid control motor, 502 first liquid extraction pump, 503 injection pipe, 504 second storage shell, 504a second liquid inlet portion, 505 second metal sheet, 505a second limiting portion, 506 first storage shell, 506a first liquid inlet portion, 507 first metal sheet, 507a first limiting portion, 508 second electromagnet, 509 mounting seat, 510 second liquid extraction pump, 600 second detection groove.
Detailed Description
Before describing the technical scheme of the present invention, the terms used herein are defined as follows:
in order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present invention is not limited to the specific embodiments disclosed below.
Further, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic can be included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1
Referring to fig. 1 to 13, in order to provide a first embodiment of the present invention, a chip capable of simultaneously detecting multiple body fluids is provided, which can realize simultaneous detection of multiple body fluids with high detection efficiency.
A chip capable of simultaneously detecting multiple body fluids comprises a chip component 100, wherein one end of the chip component 100 is provided with a first detection groove 106, a first installation channel 408 is arranged in the chip component 100, a plurality of liquid inlet channels are arranged on the chip component 100 at one end of the first installation channel 408, a plurality of installation grooves 107 are arranged on the chip component 100, the chip component 100 is connected with a liquid inlet component 300 through the installation grooves 107, a first reaction component 400 for detecting the acid and alkali of various body fluids is connected in the chip component 100, the first reaction component 400 comprises a concentrated injection seat 401 which is fixed in the chip component 100 and is arranged at one end of the first installation channel 408, one end of the concentrated injection seat 401 is provided with a plurality of concentrated injection openings 401a communicated with the liquid inlet channels, the other end of the concentrated injection seat 401 is connected with a dispersed injection seat 402, one end of the dispersed injection seat 402 opposite to the concentrated injection seat 401 is provided with a first liquid outlet groove 401b, a second liquid outlet groove 401c, a third liquid outlet groove 401d and a fourth liquid outlet groove 401e which are mutually independent, the dispersing injection seat 402 is provided with a first liquid inlet groove 402a corresponding to the first liquid outlet groove 401b, a second liquid inlet groove 402d corresponding to the second liquid outlet groove 401c, a third liquid inlet groove 402f corresponding to the third liquid outlet groove 401d and a fourth liquid inlet groove 402h corresponding to the fourth liquid outlet groove 401e at one end, far away from the concentrating injection seat 401, of the dispersing injection seat 402, a plurality of first liquid outlet holes 402b communicated with the first liquid outlet groove 401b, a second liquid outlet hole 402c communicated with the second liquid outlet groove 401c, a third liquid outlet hole 402e communicated with the third liquid outlet groove 401d and a fourth liquid outlet hole 402g communicated with the fourth liquid outlet groove 401e are arranged, a rotating ring 406 is rotatably connected in the chip assembly 100 at the other end of the first mounting channel 408, a transmission motor 405 is fixedly connected in a first mounting channel 408 of the chip assembly 100, the transmission motor 405 is connected with a rotating ring 406 through a transmission belt 407, one end of the scattered injection seat 402, which is far away from the concentrated injection seat 401, is fixedly connected with a guide shaft 404, one end of the guide shaft 404, which is far away from the scattered injection seat 402, is fixedly connected in the chip assembly 100, the rotating ring 406 is rotatably connected on the guide shaft 404, a reaction ring 403 controlled by magnetic force is slidably connected on the guide shaft 404, a plurality of injection pipes 410 which are in one-to-one correspondence with a first liquid outlet 402b, in one correspondence with a second liquid outlet 402c, in correspondence with a third liquid outlet 402e and in correspondence with a fourth liquid outlet 402g and controlled by magnetic force are arranged on the reaction ring 403, a first inflow inlet 410a and a second inflow inlet 410b which are axially arranged at intervals are arranged at the side part of the injection pipe inlet 410, a first electromagnet 409 is connected on the chip assembly 100 at the first detection groove 106, a plurality of reaction liquid storage tanks 403c which are in one-to-one correspondence with the injection pipes 410 are arranged in the reaction ring 403, a plurality of sealing ports which are in one-to-one correspondence with the reaction liquid storage tanks 403c are arranged on one side of the reaction ring 403 opposite to the rotary ring 406, sealing plugs 411 are connected at the sealing ports, reaction liquid is injected into the reaction liquid storage tanks 403c through the sealing ports, the sealing plugs 411 are connected to the reaction ring 403 through the sealing ports after injection is finished, the liquid feeding of the reaction liquid is convenient, the magnetism of the reaction ring 403 is smaller than that of the injection pipes 410, the reaction ring 403 can be inserted into the rotary ring 406, a sealing port 403b is formed on the reaction ring 403 on the side of the reaction liquid storage tanks 403c opposite to the injection pipe 410, a liquid injection cavity 403a is formed between the sealing port 403b and the inner wall of one end of the reaction ring 403 away from the reaction liquid storage tanks 403c, one end of the injection pipe 410 away from the dispersed injection seat 402 is provided with a sealing part 410c, the sealing part 410c can just plug the sealing port 403b, when the first inflow inlet 410a of the injection tube 410 is located in the corresponding first liquid outlet 402b, the second inflow inlet 410b is located in the liquid injection chamber 403 a.
Further, the liquid inlet assembly 300 comprises a liquid inlet housing 301a which is connected in a corresponding installation sinking groove 105h in a lifting manner and is provided with an upward opening, a lifting hole 105c is formed in the chip assembly 100 at the lower side of the liquid inlet assembly 300, a liquid draining part 301a-2 is arranged at the outer side of the lower part of the liquid inlet housing 301a, a liquid outlet hole 301a-1 is formed in the liquid draining part 301a-2, a pressing plate is slidably connected in the liquid inlet housing 301a, a center of the liquid inlet housing 301a is fixedly connected with a center shaft 301c, one end of the center shaft 301c extending upwards out of the liquid inlet housing 301a is fixedly connected with a fixing ring 301b, a center shaft 301c below the fixing ring 301b is fixedly connected with a fixing outer ring 301g, the periphery of the fixing outer ring 301g is attached to the inner ring of a compression plate, an extensible film is arranged between the fixing outer ring 301g and the pressing plate, the upper side of the pressing plate is flush, the film is in a lifting state, at least two linear drivers 301d are arranged on the fixing ring 301b, a linear driver 301d is arranged on the fixing ring 301d, a guide rod is arranged on the upper side of the liquid inlet housing 301b, the linear driver 301d is connected with the lower side of the liquid inlet housing 301e, the linear driver 301e is connected with the upper side of the liquid inlet housing 301e, the liquid inlet housing is connected with the lower side of the liquid inlet housing 301e, the liquid inlet housing 301e is connected with the upper side of the liquid inlet housing 301h, and the liquid inlet assembly is connected with the lower side of the liquid guide rod 301 h.
When liquid is fed, the injection needle 200 is inserted into the central shaft 301c, body fluid is injected into the central shaft 301c through the injection needle 200, the body fluid enters the liquid inlet cavity through the liquid inlet 301c-1 on the central shaft 301c, the pressing plate is moved downwards, the film is unfolded, the pressing plate and the fixed outer ring 301g are always in a sealed state, and the pressing plate moves downwards to promote the body fluid in the liquid inlet cavity to be discharged through the liquid outlet hole 301 a-1; when the body fluid is not being discharged by the downward moving platen, the body fluid can be discharged through the liquid outlet 301a-1 by connecting a peristaltic pump to the upper end of the injection needle 200.
In this embodiment, four liquid inlet channels are provided, namely, a first liquid inlet channel 105f, a second liquid inlet channel 105e, a third liquid inlet channel 105g and a fourth liquid inlet channel 105d, four liquid inlet components 300 are provided, and four liquid inlet components 300 are respectively a first liquid inlet component 304, a second liquid inlet component 303, a third liquid inlet component 302 and a fourth liquid inlet component 301, the first liquid inlet component 304 outputs urine to the first liquid inlet channel 105f, the second liquid inlet component 303 outputs sweat to the second liquid inlet channel 105e, the third liquid inlet component 302 outputs tears to the third liquid inlet channel 105g, the fourth liquid inlet component 301 outputs saliva to the fourth liquid inlet channel 105d, and the urine sequentially enters the injection pipe 410 through the first liquid inlet channel 105f, the corresponding concentrated 401a, the first liquid outlet 401b, the first liquid inlet 402a, the first liquid outlet 402b and the corresponding first inflow injection port 410 a.
The reaction ring 403, the section of the chip assembly 100 between the reaction ring 403 and the detection groove are all transparent; before detection, a plurality of lifting structures matched with the liquid inlet component 300 are arranged on the lower side of the chip, the specific structure of the lifting structure is not shown in the prior art, the lifting structure can be a lifting driver, a lifting rod which extends upwards and can do reciprocating linear movement is connected to the lifting driver, the lifting rod can just pass through the lifting hole 105c to be in contact with the bottom side of the liquid inlet shell 301a, when the liquid inlet shell 301a is abutted against the bottom of the chip component 100, the liquid outlet hole 301a-1 of the first liquid inlet component 304 is communicated with the first liquid inlet channel 105f, the liquid outlet hole 301a-1 of the second liquid inlet component 303 is communicated with the second liquid inlet channel 105e, the liquid outlet hole 301a-1 of the third liquid inlet component 302 is communicated with the third liquid inlet channel 105g, and the liquid outlet hole 301a-1 of the fourth liquid inlet component 301 is communicated with the fourth liquid inlet channel 105 d; in the initial state, the reaction ring 403 is closely attached to one side of the dispersion injection seat 402 away from the centralized injection seat 401, four liquid inlet channels are arranged, different body fluids are introduced into the corresponding liquid inlet channels through each liquid inlet component 300, the different body fluids respectively enter the centralized injection openings 401a through the corresponding liquid inlet channels, the body fluids flowing out of the four centralized injection openings 401a respectively enter the first liquid outlet groove 401b, the second liquid outlet groove 401c, the third liquid outlet groove 401d and the fourth liquid outlet groove 401e, the body fluids in the first liquid outlet groove 401b are divided into a plurality of paths to be respectively output from the plurality of first liquid outlet holes 402b, the body fluids respectively enter the injection pipes 410 through the corresponding first inflow injection openings 410a, the body fluids in the second liquid outlet groove 401c sequentially enter the corresponding injection pipes 410 through the second liquid outlet holes 402c and the corresponding first inflow openings 410a, the body fluids in the third liquid outlet groove 401d sequentially enter the corresponding injection pipes 410 through the third liquid outlet holes 402e and the corresponding first inflow openings 410a, the body fluid in the fourth liquid outlet groove 401e sequentially enters the corresponding injection pipe 410 through the fourth liquid outlet 402g and the corresponding first inflow injection opening 410a, the liquid inlet is finished, the first electromagnet 409 is electrified, the strength of the first electromagnet 409 is regulated, the injection pipe 410 is sucked into the corresponding reaction liquid storage tank 403c, the reaction ring 403 is fixed, the outer end of the injection pipe 410 just seals the reaction ring 403, the body fluid reacts with the reaction liquid in the reaction liquid storage tank 403c, the strength of the first electromagnet 409 is increased, the reaction ring 403 slides along the guide shaft 404 towards the direction of the rotation ring 406 until the reaction ring 403 slides onto the rotation ring 406, the transmission motor 405 acts, the rotation ring 406 is driven by the transmission belt 407, after the body fluid and the reaction liquid are fully mixed, the reaction liquid storage tank 403c sequentially aligns with the color sensor, the color sensor sequentially recognizes the color change after the reaction, for analysis; the invention realizes the simultaneous detection of various body fluids and improves the detection efficiency through the combined arrangement of the first reaction component 400 and the chip component 100.
Example 2
Referring to fig. 14 to 21, in a second embodiment of the present invention, a chip capable of detecting multiple body fluids simultaneously is provided, which is different from the second embodiment in that the detection of multiple proteins in saliva can be realized.
The chip capable of detecting various body fluids simultaneously further comprises a second reaction component 500, the second reaction component 500 is arranged on the chip component 100 between the fourth liquid inlet component 301 and the third liquid inlet component 302, a second installation channel is further arranged in the chip component 100, the second reaction component 500 comprises a first storage shell 506 and a plurality of second storage shells 504 which are connected in the second installation channel, a vertically arranged reaction liquid flow channel is arranged in the chip component 100, the inner cavity of the first storage shell 506 can be communicated with the reaction liquid flow channel, the inner cavity of the second storage shell 504 can be communicated with the reaction liquid flow channel, the inner cavity of the first storage shell 506 can be communicated with the liquid inlet cavity in one of the liquid inlet components 300, a plurality of groups of reaction units connected end to end are further arranged in the chip component 100, each reaction unit comprises a first reaction channel, one end of the first reaction channel in the reaction units at the head end is communicated with the reaction liquid flow channel, one end of the second reaction channel is connected with one end of the second reaction channel, the other end of the second reaction channel is communicated with one end of the third reaction channel, and the other end of the first reaction channel at the front end is communicated with one end of the next first reaction channel.
In order to further realize simultaneous detection of multiple proteins in saliva, a reaction liquid control motor 501 is fixedly connected in the chip assembly 100, a liquid injection pipe 503 extending downwards is connected on the reaction liquid control motor 501, a first liquid inlet portion 506a is arranged on one side of the first storage shell 506, which is opposite to the liquid injection pipe 503, a second liquid inlet portion 504a is arranged on one side of the second storage shell 504, which is opposite to the liquid injection pipe 503, one end of the liquid injection pipe 503 can be attached to the outside of the first liquid inlet portion 506a and aligned to the first liquid inlet portion 506a or attached to the outside of the second liquid inlet portion 504a and aligned to the second liquid inlet portion 504a, the other end of the liquid injection pipe 503 is fixedly connected with a first liquid extraction pump 502, the lower end of the liquid injection pipe 503 is connected with the upper end of a reaction liquid flow channel, a plurality of reaction tanks 105b-5 are arranged in the chip assembly 100 between the first reaction channels and the second reaction channels in parallel, a reaction liquid tank 105b-4 is arranged on the chip assembly 100 on one side of the first reaction channels and the third reaction channels, a liquid extraction hole 105b-4 is formed in the chip assembly 100 on one side of the first reaction channels and the third reaction channels faces the reaction tanks 105b-5, one end of the other end of the third reaction channels is provided with a liquid extraction pump 510, and the other end of the waste liquid in the third reaction channels is far away from the chip assembly is connected with the waste liquid pump 100, and the waste liquid in the chip assembly is connected to the other end of the first reaction channels.
Further, the chip assembly 100 includes a first chip body 105, a second chip body 104, a third chip body 103, a fourth chip body 102 and a fifth chip body 101 which are sequentially connected together from bottom to top, and one end of a guide shaft 404, which is far away from the scattered injection seat 402, is fixedly connected in the first chip body 105; four connection countersinks 107e in rectangular arrangement are arranged on one side of the first chip body 105 facing upwards, four first connection ports 107d in rectangular arrangement are arranged on the second chip body 104, four second connection ports 107c in rectangular arrangement are arranged on the third chip body 103, four third connection ports 107b in rectangular arrangement are arranged on the fourth chip body 102, four fourth connection ports 107a in rectangular arrangement are arranged on the fifth chip body 101, and the fourth connection ports 107a, the third connection ports 107b, the second connection ports 107c, the first connection ports 107d and the corresponding connection countersinks 107e form mounting grooves 107; the first detection groove 106 is provided at the side ends of the first chip body 105, the second chip body 104, and the third chip body 103; a lower waste liquid flow channel 102c is arranged on the upward side of the first chip body 105, an upper waste liquid flow channel 104d corresponding to the lower waste liquid flow channel 102c is arranged on the downward side of the second chip body 104, and a waste liquid pool is formed by the lower waste liquid flow channel 102c and the upper waste liquid flow channel 104 d; the fourth chip body 102 is provided with a first vertical through hole 102a aligned with the vertical section of the liquid injection pipe 503, the third chip body 103 is provided with a second vertical through hole 103a coaxial with the first vertical through hole 102a, the second chip body 104 is provided with a third vertical through hole 104a coaxial with the second vertical through hole 103a, one side of the first chip body 105 facing upwards is provided with a reaction liquid inlet sedimentation tank 105a, the first vertical through hole 102a, the second vertical through hole 103a, the third vertical through hole 104a and the reaction liquid inlet sedimentation tank 105a form a reaction liquid flow channel, one side of the first chip body 105 facing upwards is provided with a mounting sedimentation tank 105h, the second chip body 104 is provided with a mounting port 104c corresponding to the mounting sedimentation tank 105h, the mounting sedimentation tank 105h and the mounting port 104c form a first mounting channel 408, the third chip body 103 seals the first mounting channel 408, a plurality of groups of lower reaction units 105b which are connected end to end are arranged on one side of the first chip body 105 which faces upwards, the lower reaction units 105b comprise two groups of first lower reaction liquid flow channels 105b-3, second lower reaction liquid flow channels 105b-2 and third lower reaction liquid flow channels 105b-1 which are connected together in sequence, a plurality of groups of upper reaction units 104b which are connected end to end are arranged on one downward end of the second chip body 104, the upper reaction units 104b comprise two groups of first upper reaction liquid flow channels 104b-2, second upper reaction liquid flow channels 104b-1 and third lower reaction liquid flow channels 105b-1 which are connected in sequence, a first reaction channel with a closed upper end and a closed lower end is formed between the first lower reaction liquid flow channels 105b-3 and the corresponding first upper reaction liquid flow channels 104b-2, a second reaction channel with a closed upper end and a closed lower end is formed between the second lower reaction liquid flow channels 105b-2 and the corresponding second upper reaction liquid flow channels 104b-1, a third reaction channel with closed upper and lower ends is formed between a third lower reaction liquid flow channel 105b-1 and a corresponding third upper reaction liquid flow channel 104b-3, a detection chamber 105b-6 is formed on the upward side of the first chip body 105 and the downward side of the second chip body 104 between the first reaction channel and the third reaction channel, a reaction liquid drain hole 105b-4 is arranged on the first chip body 105 on the side of the first reaction channel and the third reaction channel facing the detection chamber 105b-6, reaction tanks 105b-5 are all arranged on the first chip body 105 corresponding to the position of the detection chamber 105b-6, different antibodies are coated in the reaction tanks 105b-5 in different reaction units, a connecting groove is arranged at the downward end of the fifth chip body 101, a reaction liquid control motor 501 is fixedly connected in the fifth chip body 101 through the connecting groove, a liquid injection pipe 503 is rotatably connected in the fourth chip body 102, a first upper liquid outlet channel 101c is arranged on the downward side of the fifth chip body 101 close to the first storage shell 506, a first lower liquid outlet channel 102b corresponding to the first upper liquid outlet channel 101c is arranged on the upward side of the fourth chip body 102, a first liquid outlet channel is formed by the first upper liquid outlet channel 101c and the first lower liquid outlet channel 102b, one end of the first liquid outlet channel can be aligned with a liquid outlet hole 301a-1 of a liquid inlet shell 301a in the fourth liquid inlet assembly 301, one end of the first liquid inlet channel 105f, one end of the second liquid inlet channel 105e, one end of the third liquid inlet channel 105g and one end of the fourth liquid inlet channel 105d are respectively communicated with a liquid outlet hole 301a-1 on the corresponding liquid inlet shell 301a, a first lower liquid inlet channel, a second lower liquid inlet channel, a third lower liquid inlet channel and a fourth lower liquid inlet channel are arranged on the upward side of the first chip body 105, the second chip body 104 is provided with a first upper liquid inlet channel corresponding to the first lower liquid inlet channel, a second upper liquid inlet channel corresponding to the second lower liquid inlet channel, a third upper liquid inlet channel corresponding to the third lower liquid inlet channel and a fourth upper liquid inlet channel corresponding to the fourth lower liquid inlet channel on the downward side, the first lower liquid inlet channel and the first upper liquid inlet channel form a first liquid inlet channel 105f, the second lower liquid inlet channel and the second upper liquid inlet channel form a second liquid inlet channel 105e, the third lower liquid inlet channel and the first third liquid inlet channel form a third liquid inlet channel 105g, the fourth lower liquid inlet channel and the fourth upper liquid inlet channel form a fourth liquid inlet channel 105d, the other end of the first liquid outlet channel is connected with one end of the first storage shell 506 away from the first liquid inlet portion 506a, the fourth chip body 102 is internally sliding connected with a mounting seat 509, the mounting seat 509 is fixedly arranged on one side of the liquid injection tube 503 away from one end of the first liquid pump 502, the second electromagnet 508 is fixedly connected in the mounting seat 509, one end of the first storage shell 506, which is close to the first liquid inlet portion 506a, is connected with the first metal sheet 507 in a lifting manner, a first limiting portion 507a is arranged on the upper portion of the first metal sheet 507, in an initial state, the first limiting portion 507a is abutted against the upper side of the first storage shell 506, the first metal sheet 507 separates the inner cavity of the first storage shell 506 from the first liquid inlet portion 506a, one end of the second storage shell 504, which is close to the second liquid inlet portion 504a, is connected with the second metal sheet 505 in a lifting manner, a second limiting portion 505a is arranged on the upper portion of the second metal sheet 505, in an initial state, the second limiting portion 505a is abutted against the upper side of the second storage shell 504, the second metal sheet 505 separates the inner cavity of the second storage shell 504 from the second liquid inlet portion 504a, and the second electromagnet 508 can be aligned with the first metal sheet 507 or the second metal sheet 505.
In the initial state, the second liquid inlet component 303 is lifted by the liquid injection pipe 503 through the external lifting driver, when the liquid outlet hole 301a-1 moves up to be aligned with one end of the first liquid outlet channel, the lifting driver stops acting, saliva is introduced into the liquid inlet cavity through the injection needle 200, and the saliva sequentially passes through the liquid outlet hole 301a-1 and the first liquid outlet channel to enter the first storage shell 506; the side part of the third chip body 103 and the side part of the fourth chip body 102 are respectively provided with a first detection sink 103b and a second detection sink, the first detection sink 103b and the second detection sink form a second detection sink 600, the second detection sink 600 covers the area where each reaction unit is located, four groups of reaction units are arranged in the embodiment, and each group of reaction units realizes detection of different proteins in saliva; in order to facilitate the liquid feeding in the second storage case 504, a reaction liquid inlet hole 101a is formed in an upward side of the second storage case 504, a plurality of injection holes corresponding to the reaction liquid inlet holes 101a one by one are arranged on the fifth chip body 101, a plurality of liquid injection parts 101b corresponding to the injection holes one by one are arranged on the lower side of the fifth chip body 101, and the liquid injection parts 101b are inserted into the reaction liquid inlet holes 101a corresponding to the second storage case 504.
Taking transferrin in saliva as an example, the detection method comprises the following steps:
(1) Controlling the second electromagnet 508 to act so as to enable the first metal sheet 507 to move upwards, enabling the first liquid inlet part 506a to be communicated with the liquid injection pipe 503, enabling the first liquid pumping pump 502 to act, sucking saliva in the first storage shell 506, enabling the saliva to sequentially enter the first reaction channel, the second reaction channel and the third reaction channel through the reaction liquid outlet channel, enabling the saliva to enter the reaction tank 105b-5 of the detection chamber 105b-6 through the reaction liquid outlet hole 105b-4, enabling the saliva to end in a liquid discharging mode, enabling the second electromagnet 508 to be closed, enabling the first metal sheet 507 to move downwards, and separating the first liquid inlet part 506a from the inner cavity of the first storage shell 506 again;
(2) The reaction liquid controls the motor 501 to act, the liquid injection pipe 503 rotates to align with the position of the next second liquid inlet part 504a, the reaction liquid controls the motor 501 to stop acting, the second electromagnet 508 is started, the second metal sheet 505 moves upwards to be opened, the first liquid pumping pump 502 is started, the detergent in the second storage shell 504 is sucked, the detergent flows into the reaction tank 105b-5, unbound sample is removed, the second electromagnet 508 is closed, the first liquid pumping pump 502 is closed, the second liquid pumping pump 510 is opened, and redundant waste liquid is pumped into the waste liquid tank;
(3) The reaction liquid controls the motor 501 to act, the liquid injection pipe 503 is aligned with the next second liquid inlet part 504a, the reaction liquid controls the motor 501 to stop acting, the second electromagnet 508 is started, the second metal sheet 505 moves upwards to be opened, the first liquid pumping pump 502 is started, the biotin-modified antibody in the biotin-modified second storage shell 504 is sucked, the biotin-modified antibody enters the transferrin detection chamber 105b-6 at the head end for incubation, the incubation is finished, the second electromagnet 508 and the first liquid pumping pump 502 are closed, the second metal sheet 505 moves downwards, the second liquid pumping pump 510 is opened, and redundant waste liquid is pumped into a waste liquid pool;
(4) The reaction liquid controls the motor 501 to act, the liquid injection pipe 503 rotates to align with the position of the next second liquid inlet part 504a, the reaction liquid controls the motor 501 to stop acting, the second electromagnet 508 is started, the second metal sheet 505 moves upwards to be opened, the first liquid pumping pump 502 is started, the detergent in the second storage shell 504 is sucked, the detergent flows into the reaction tank 105b-5, redundant biotin antibody is removed, the second electromagnet 508 is closed, the first liquid pumping pump 502 is closed, the second liquid pumping pump 510 is opened, and redundant waste liquid is pumped into the waste liquid tank;
(5) The reaction liquid controls the motor 501 to act, the liquid injection pipe 503 rotates to align with the position of the next second liquid inlet part 504a, the reaction liquid controls the motor 501 to stop acting, the second electromagnet 508 is started, the second metal sheet 505 moves upwards to be opened, the first liquid pumping pump 502 is started, the horseradish peroxidase reaction liquid containing avidin marks in the second storage shell 504 is sucked, the horseradish peroxidase reaction liquid containing avidin marks flows into the reaction tank 105b-5, the reaction is finished, the second electromagnet 508 is closed, the first liquid pumping pump 502 is closed, the second liquid pumping pump 510 is opened, and redundant waste liquid is pumped into the waste liquid tank;
(6) The reaction liquid controls the motor 501 to act, the liquid injection pipe 503 rotates to align with the position of the next second liquid inlet part 504a, the reaction liquid controls the motor 501 to stop acting, the second electromagnet 508 is started, the second metal sheet 505 moves upwards to be opened, the first liquid pumping pump 502 is started, the reaction liquid containing tetramethyl benzidine substrate in the second storage shell 504 is sucked, the reaction liquid containing tetramethyl benzidine substrate flows into the reaction tank 105b-5, the color reaction occurs, the reaction is finished, the second electromagnet 508 is closed, the first liquid pumping pump 502 is closed, the second liquid pumping pump 510 is opened, and the redundant waste liquid is pumped into the waste liquid tank;
(7) The reaction liquid controls the motor 501 to act, the liquid injection pipe 503 rotates to the position of the second liquid inlet part 504a aligned with the tail end, the reaction liquid controls the motor 501 to stop acting, the second electromagnet 508 is started, the second metal sheet 505 moves upwards to be opened, the first liquid pumping pump 502 is started, the stopping liquid in the second storage shell 504 is sucked, the stopping liquid flows into the reaction tank 105b-5, the chromogenic reaction is stopped, the reaction is ended, the second electromagnet 508 is closed, the first liquid pumping pump 502 is closed, the second liquid pumping pump 510 is opened, and the redundant waste liquid is pumped into the waste liquid tank;
(8) A light source facing the second detection groove 600 is placed at the lower side of the first chip body 105, a colorimetric camera is inserted into the second detection groove 600, and the colorimetric camera shoots a color development condition, so that detection of transferrin in saliva is realized.
The invention realizes the detection of different proteins in saliva through the arrangement of the second reaction component 500 and the liquid inlet component 300.
It should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted without departing from the spirit and scope of the technical solution of the present invention, which is intended to be covered in the scope of the claims of the present invention.
Claims (10)
1. A chip capable of simultaneously detecting multiple body fluids, characterized in that: it comprises
The chip assembly (100), one end of the chip assembly (100) is provided with a first detection groove (106), a first installation channel (408) is arranged in the chip assembly (100), a plurality of liquid inlet channels are arranged on the chip assembly (100) at one end of the first installation channel (408), and a plurality of installation grooves (107) are arranged on the chip assembly (100);
the liquid inlet assembly (300) is provided with a plurality of groups of liquid inlet assemblies (300) and corresponds to the mounting grooves (107) one by one, and the liquid inlet assemblies (300) are mounted in the corresponding mounting grooves (107);
the first reaction component (400), the first reaction component (400) comprises a concentrated injection seat (401) fixed in the chip component (100) and arranged at one end of a first installation channel (408), one end of the concentrated injection seat (401) is provided with a plurality of concentrated injection inlets (401 a) communicated with the liquid inlet channel, the other end of the concentrated injection seat (401) is connected with a dispersed injection seat (402), one end of the dispersed injection seat (402) opposite to the concentrated injection seat (401) is provided with a first liquid outlet groove (401 b), a second liquid outlet groove (401 c), a third liquid outlet groove (401 d) and a fourth liquid outlet groove (401 e) which are mutually independent, one end of the dispersed injection seat (402) far away from the concentrated injection seat (401) is provided with a plurality of first liquid outlet holes (402 b) communicated with the first liquid outlet groove (401 b), a second liquid outlet hole (402 c) communicated with the second liquid outlet groove (401 c), a third liquid outlet hole (402 e) communicated with the third liquid outlet groove (401 d) and a fourth liquid outlet groove (401 e) which are mutually independent, one end of the dispersed injection seat (402) is far away from the concentrated injection seat (402) is provided with a first liquid outlet groove (401) communicated with the first liquid outlet groove (401 b), the first rotary ring (402) is connected with the first liquid outlet seat (402) far away from the concentrated injection seat (408), one end fixed connection that dispersion was kept away from to guiding axle (404) is gone into seat (402) is in chip subassembly (100), be connected with on guiding axle (404) by magnetic force control and can peg graft reaction ring (403) on rotatory ring (406) with, a plurality of injection pipes (410) that just receive magnetic force control with each liquid outlet one-to-one of arranging on reaction ring (403), first inflow filling opening (410 a) and second inflow filling opening (410 b) that axial interval set up are opened to injection pipe (410) lateral part, are connected with first electro-magnet (409) on chip subassembly (100) of first detection groove (106) department, a plurality of reaction liquid storage tanks (403 c) with injection pipe (410) one-to-one have been arranged in reaction ring (403), the magnetism of reaction ring (403) is less than the magnetism of injection pipe (410).
2. The chip capable of simultaneously detecting multiple body fluids according to claim 1, wherein: the reaction ring (403) on one side of the reaction liquid storage pool (403 c) opposite to the injection pipe (410) is provided with a sealing port (403 b), an injection cavity (403 a) is formed between the sealing port (403 b) and the inner wall of one end of the reaction ring (403) away from the reaction liquid storage pool (403 c), one end of the injection pipe (410) away from the dispersion injection seat (402) is provided with a sealing part (410 c), the sealing part (410 c) can just seal the sealing port (403 b), and when a first inflow injection port (410 a) of the injection pipe (410) is in a corresponding first liquid outlet (402 b), a second inflow injection port (410 b) is in the injection cavity (403 a).
3. The chip capable of simultaneously detecting multiple body fluids according to claim 1, wherein: the liquid feeding assembly (300) comprises a liquid feeding shell (301 a) which is connected in a corresponding installation sinking groove (105 h) in a lifting manner and provided with an upward opening, a pressing plate is connected in the liquid feeding shell (301 a) in a sliding manner, a center of the liquid feeding shell (301 a) is fixedly connected with a center shaft (301 c), one end of the center shaft (301 c) extending out of the liquid feeding shell (301 a) upward is fixedly connected with a fixing ring (301 b), at least two linear drivers (301 d) are arranged on the fixing ring (301 b), a telescopic rod (301 h) capable of performing reciprocating linear movement in the height direction is connected to the linear drivers (301 d), the lower end of the telescopic rod (301 h) is connected to the upper side of the pressing plate, an upward liquid feeding counter bore is formed in the center shaft (301 c), a liquid feeding port (301 c-1) is formed in the side portion of the center shaft (301 c) below the pressing plate, and a liquid feeding cavity is formed between the lower side of the pressing plate and the inner wall of the liquid feeding shell (301 a).
4. A chip capable of simultaneously detecting a plurality of body fluids as claimed in any one of claims 1 to 3, wherein: still include second reaction subassembly (500), still be equipped with the second installation passageway in chip subassembly (100), second reaction subassembly (500) are including connecting first storage shell (506) and a plurality of second storage shell (504) in the second installation passageway, be equipped with the reaction play liquid runner of vertical setting in chip subassembly (100), the inner chamber of first storage shell (506) can with the reaction play liquid runner intercommunication, the inner chamber of second storage shell (504) can with the reaction play liquid runner intercommunication, the inner chamber of first storage shell (506) can with the feed liquor chamber intercommunication in one of them feed liquor subassembly (300), still be equipped with a plurality of groups end to end's reaction unit in chip subassembly (100), the reaction unit includes first reaction channel, first reaction channel one end and the reaction play liquid runner intercommunication in the reaction unit of first end, the one end of second reaction channel is connected to the first reaction channel other end, the other end and the third reaction channel one end intercommunication of second reaction channel, in the two adjacent reaction units, the first reaction channel other end and the one end intercommunication of next first reaction channel.
5. The chip capable of simultaneously detecting multiple body fluids according to claim 4, wherein: the chip assembly is characterized in that a reaction liquid control motor (501) is fixedly connected in the chip assembly (100), a liquid injection pipe (503) extending downwards is connected to the reaction liquid control motor (501), a first liquid inlet (506 a) is arranged on one side of a first storage shell (506) opposite to the liquid injection pipe (503), a second liquid inlet (504 a) is arranged on one side of a second storage shell (504) opposite to the liquid injection pipe (503), one end of the liquid injection pipe (503) can be attached to the outer side of the first liquid inlet (506 a) and aligned with the first liquid inlet (506 a) or attached to the outer side of the second liquid inlet (504 a) and aligned with the second liquid inlet (504 a), a first liquid pumping pump (502) is fixedly connected to the other end of the liquid injection pipe (503), and the lower end of the liquid injection pipe (503) is connected with the upper end of a reaction liquid outlet runner.
6. The chip capable of simultaneously detecting multiple body fluids according to claim 4, wherein: the first reaction channels and the second reaction channels are arranged in parallel, a plurality of reaction tanks (105 b-5) are arranged in the chip assembly (100) between the first reaction channels and the second reaction channels, and reaction liquid drain holes (105 b-4) are formed in the chip assembly (100) on one side, facing the reaction tanks (105 b-5), of the first reaction channels and the third reaction channels.
7. The chip capable of simultaneously detecting multiple body fluids according to claim 4, wherein: a waste liquid outlet channel is arranged in a chip assembly (100) at the other end of the third reaction channel in the tail end reaction unit, a waste liquid pool is arranged in the chip assembly (100), and one end, far away from the tail end, of the third reaction channel of the waste liquid outlet channel is connected with the waste liquid pool.
8. The chip capable of simultaneously detecting multiple body fluids according to claim 7, wherein: a second liquid pump (510) is connected to the chip assembly (100) at one end of the waste liquid pool far away from the reaction unit.
9. The chip capable of simultaneously detecting multiple body fluids according to claim 5, wherein: the chip assembly (100) comprises a first chip body (105), a second chip body (104) and a third chip body which are sequentially connected from bottom to top, wherein one side of the first chip body (105) upwards is provided with a mounting sink groove (105 h), the second chip body (104) is provided with a mounting opening (104 c) corresponding to the mounting sink groove (105 h), the mounting sink groove (105 h) and the mounting opening (104 c) form a first mounting channel (408), the third chip body (103) seals the first mounting channel (408), one side of the first chip body (105) upwards is provided with a plurality of groups of lower reaction units (105 b) which are connected end to end, the lower reaction units (105 b) comprise a first lower reaction liquid runner (105 b-3), a second lower reaction liquid runner (105 b-2) and a third lower reaction liquid runner (105 b-1) which are sequentially connected together, one end of the second chip body (104) is provided with a plurality of groups of upper reaction units (104 b) which are connected end to end, the first reaction units (104 b) are connected with the first reaction liquid runner (2) and the first reaction liquid runner (104 b) are formed between the first reaction units (104 b-2) and the first reaction liquid runner (2), a second reaction channel with a closed upper and lower end is formed between the second lower reaction liquid flow channel (105 b-2) and the corresponding second upper reaction liquid flow channel (104 b-1), and a third reaction channel with a closed upper and lower end is formed between the third lower reaction liquid flow channel (105 b-1) and the corresponding third upper reaction liquid flow channel (104 b-3).
10. A chip capable of simultaneously detecting a plurality of body fluids as claimed in any one of claims 1 to 3, characterized in that: a transmission motor (405) is fixedly connected in a first mounting channel (408) of the chip assembly (100), and the transmission motor (405) is connected with a rotating ring (406) through a transmission belt (407).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210703393.7A CN115069316B (en) | 2022-06-21 | 2022-06-21 | Chip capable of detecting multiple body fluids simultaneously |
Applications Claiming Priority (1)
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