CN115069316A

CN115069316A - Chip capable of simultaneously detecting multiple body fluids

Info

Publication number: CN115069316A
Application number: CN202210703393.7A
Authority: CN
Inventors: 尹彬沣; 孙枢尧; 江卓奥; 万心华
Original assignee: Yangzhou University
Current assignee: Yangzhou Yixin 3d Technology Co ltd
Priority date: 2022-06-21
Filing date: 2022-06-21
Publication date: 2022-09-20
Anticipated expiration: 2042-06-21
Also published as: CN115069316B

Abstract

The invention discloses a chip capable of detecting various body fluids simultaneously, which comprises a chip assembly, a plurality of groups of liquid inlet assemblies and a first reaction assembly, wherein a plurality of liquid inlet channels are distributed on the chip assembly, and a plurality of mounting grooves are distributed on the chip assembly; the liquid inlet assemblies are arranged in the corresponding mounting grooves; the first reaction assembly comprises a concentrated injection seat, one end of the concentrated injection seat is provided with a plurality of concentrated injection ports communicated with the liquid inlet channels, the other end of the concentrated injection seat is connected with a dispersed injection seat, one end of the dispersed injection seat is provided with a plurality of liquid outlet grooves which are mutually independent, the other end of the dispersed injection seat is provided with a plurality of liquid outlet grooves which are in one-to-one correspondence with the liquid outlet grooves, a rotating ring is rotatably connected in the chip assembly at the other end of the first installation channel, a guide shaft is fixed in the chip assembly, and the guide shaft is slidably connected with a reaction ring which is controlled by magnetic force and can be inserted into the rotating ring; the invention can realize the simultaneous detection of a plurality of body fluids.

Description

Chip capable of detecting multiple body fluids simultaneously

Technical Field

The invention relates to the technical field of cancer in-vitro detection, in particular to a chip capable of detecting various body fluids simultaneously.

Background

The microfluidic technology is used for detecting body fluids such as saliva, urine, tears and the like, the sampling amount is small, and the method is suitable for field detection.

Among the prior art, the name of disclosing is "a micro-fluidic saliva detects chip", and publication number is CN 215996712U, and the publication date is 2022.03.11's chinese utility model patent, and it includes upper basement and lower floor's basement, lower floor's basement is fixed to be set up in upper basement bottom, upper basement left side detachable is provided with slidingtype filter mechanism, support plate among the slidingtype filter mechanism slides and sets up the holding tank inboard in slidingtype filter mechanism, and it can conveniently realize salivary detection, nevertheless can't realize the while of multiple body fluid and detect, the function singleness.

Disclosure of Invention

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made in view of the above and/or other problems occurring in the conventional body fluid testing.

Therefore, an object of the present invention is to provide a chip capable of simultaneously detecting a plurality of body fluids, which can realize simultaneous detection of a plurality of body fluids.

In order to solve the technical problems, the invention provides the following technical scheme: a chip capable of simultaneously detecting a plurality of body fluids, comprising,

the liquid level detection device comprises a chip assembly, a liquid level detection device and a liquid level detection device, wherein a first detection groove is formed in one end of the chip assembly, a first installation channel is arranged in the chip assembly, a plurality of liquid inlet channels are distributed on the chip assembly at one end of the first installation channel, and a plurality of installation grooves are distributed on the chip assembly;

the liquid inlet assemblies are provided with a plurality of groups and correspond to the mounting grooves one by one, and the liquid inlet assemblies are mounted in the corresponding mounting grooves;

the first reaction assembly comprises a centralized injection seat which is fixed in the chip assembly and is installed at one end of a first installation channel, a plurality of centralized injection ports communicated with the liquid inlet channel are arranged at one end of the centralized injection seat, a dispersed injection seat is connected at the other end of the centralized injection seat, a first liquid outlet groove, a second liquid outlet groove, a third liquid outlet groove and a fourth liquid outlet groove which are mutually independent are arranged at one end of the dispersed injection seat opposite to the centralized injection seat, a plurality of first liquid outlets communicated with the first liquid outlet groove, a second liquid outlet communicated with the second liquid outlet groove, a third liquid outlet communicated with the third liquid outlet groove and a fourth liquid outlet communicated with the fourth liquid outlet groove are arranged at one end of the dispersed injection seat far away from the centralized injection seat, a rotating ring is rotatably connected in the chip assembly at the other end of the first installation channel, and a guide shaft is fixedly connected at one end of the dispersed injection seat far away from the centralized injection seat, one end fixed connection that the dispersion was kept away from to the guiding axle and is injected the seat is in the chip subassembly, be connected with the reaction ring that receives magnetic control on the guiding axle slidable, the last a plurality of and first liquid outlet one-to-one that have arranged of reaction ring corresponds with the second liquid outlet, corresponds with the third liquid outlet, and the injection pipe that receives magnetic control that corresponds with the fourth liquid outlet, the lateral part of injection pipe is opened has first influx filling opening and the second influx filling opening that axial interval set up, is connected with first electro-magnet on the chip subassembly of first detection groove department, the reaction intra-annular a plurality of and the reaction liquid storage pool of injection pipe one-to-one that have arranged, and the magnetism of reaction ring is less than the magnetism of injection pipe, the reaction ring can be pegged graft on rotatory ring.

The sections of the reaction ring, the chip components between the reaction ring and the detection groove are transparent; in an initial state, the reaction ring is tightly attached to one side, away from the concentrated injection seat, of the dispersed injection seat, four liquid inlet channels are arranged, different body fluids are introduced into the corresponding liquid inlet channels through the liquid inlet assemblies, the different body fluids enter the concentrated injection ports through the corresponding liquid inlet channels, the body fluids flowing out of the four concentrated injection ports enter the first liquid outlet groove, the second liquid outlet groove, the third liquid outlet groove and the fourth liquid outlet groove respectively, the body fluids in the first liquid outlet groove are divided into a plurality of paths to be output from the first liquid outlets and enter the injection pipes through the corresponding first flow-in injection ports respectively, the body fluids in the second liquid outlet groove enter the corresponding injection pipes through the second liquid outlet and the corresponding first flow-in injection ports in sequence, the body fluids in the third liquid outlet groove enter the corresponding injection pipes through the third liquid outlet injection ports and the corresponding first flow-in injection ports in sequence, and the body fluids in the fourth liquid outlet groove enter the corresponding injection pipes through the fourth liquid outlet and the corresponding first flow-in injection ports in sequence When liquid feeding is finished, the first electromagnet is electrified, the strength of the first electromagnet is adjusted, the injection pipe is sucked into the corresponding reaction liquid storage tank, the reaction ring is fixed, the outer end of the injection pipe just seals the reaction ring, body fluid reacts with the reaction liquid in the reaction liquid storage tank, the strength of the first electromagnet is increased, the reaction ring slides along the guide shaft towards the direction of the rotating ring until the reaction ring slides onto the rotating ring, the rotating ring rotates, the rotating ring stops rotating after the body fluid and the reaction liquid are fully mixed, a color sensor can be arranged in the detection tank during detection, the rotating ring rotates, the reaction liquid is aligned to the color sensor, and the color change after reaction is identified so as to be analyzed; according to the invention, through the combined arrangement of the first reaction component and the chip component, the simultaneous detection of various body fluids is realized, and the detection efficiency is improved.

As a preferable embodiment of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: the reaction ring on one side of the reaction liquid storage pool, which is arranged opposite to the injection pipe, is provided with a sealing opening, a liquid injection cavity is formed between the sealing opening and the inner wall of one end, far away from the reaction liquid storage pool, of the reaction ring, a sealing part is arranged at one end, far away from the dispersed injection seat, of the injection pipe, the sealing part can just block the sealing opening, and when a first inflow injection opening of the injection pipe is in a corresponding first liquid outlet, a second inflow injection opening is in the liquid injection cavity.

As a preferable embodiment of the chip capable of simultaneously detecting a plurality of body fluids according to the present invention, wherein: the feed liquor subassembly includes that the liftable ground is connected and is being corresponded the heavy inslot of installation and have open-ended feed liquor shell up, slidable ground is connected with the clamp plate in the feed liquor shell, the central fixedly connected with axis of feed liquor shell, the solid fixed ring of one end fixedly connected with that stretches out the feed liquor shell in the axis upwards, two at least sharp drivers of having arranged on the fixed ring, be connected with the telescopic link that can be reciprocal rectilinear movement in the direction of height on the sharp driver, the lower extreme of telescopic link is connected at the clamp plate upside, it has up feed liquor counter bore to epaxial the opening, the axis lateral part of clamp plate below is opened there is the inlet, forms the feed liquor chamber between clamp plate downside and the feed liquor shell inner wall.

As a preferable embodiment of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: still include the second reaction assembly, still be equipped with second installation passageway in the chip subassembly, the second reaction assembly includes first storage shell and a plurality of second storage shell of connection in second installation passageway, be equipped with the reaction play liquid runner of vertical setting in the chip subassembly, the inner chamber of first storage shell can communicate with reaction play liquid runner, the inner chamber of second storage shell can communicate with reaction play liquid runner, the inner chamber of first storage shell can communicate with the feed liquor chamber in one of them feed liquor subassembly, still is equipped with the reaction unit of a plurality of groups end to end in the chip subassembly, the reaction unit includes first reaction channel, first reaction channel one end and reaction play liquid runner intercommunication in the reaction unit of head end, the one end of second reaction channel is connected to the first reaction channel other end, the other end and the third reaction channel one end of second reaction channel communicate, in two adjacent reaction units, the other end of the previous third reaction channel is communicated with one end of the next first reaction channel.

As a preferable embodiment of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: fixedly connected with reaction liquid control motor in the chip subassembly, be connected with the notes liquid pipe that stretches out down on the reaction liquid control motor, one side that the liquid pipe set up is annotated relatively to first storage shell is equipped with first liquid portion of advancing, one side that the liquid pipe set up is annotated relatively to the second storage shell is equipped with second liquid portion of advancing, the one end of annotating the liquid pipe can be laminated in first liquid portion outside and aim at first liquid portion or the laminating just aims at second liquid portion outside and aim at second liquid portion, annotate the first drawing liquid pump of other end fixedly connected with of liquid pipe, the lower extreme of annotating the liquid pipe and the upper end that the reaction goes out the liquid runner meet.

As a preferable embodiment of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: the first reaction channel and the second reaction channel are arranged in parallel, a plurality of reaction pools are arranged in the chip assembly between the first reaction channel and the second reaction channel, and reaction liquid drain holes are formed in the chip assembly on one side, facing the reaction pools, of the first reaction channel and the third reaction channel.

As a preferable embodiment of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: and a waste liquid outlet channel is arranged in the chip assembly at the other end of the third reaction channel in the tail end reaction unit, a waste liquid pool is arranged in the chip assembly, and one end, far away from the tail end of the third reaction channel, of the waste liquid outlet channel is connected with the waste liquid pool.

As a preferable embodiment of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: and a second liquid pump is connected in the chip assembly at one end of the waste liquid pool, which is far away from the reaction unit.

As a preferable embodiment of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: the chip assembly comprises a first chip body and a second chip body which are sequentially connected together from bottom to top, wherein an installation sinking groove is formed in one upward side of the first chip body, an installation opening corresponding to the installation sinking groove is formed in the second chip body, the installation sinking groove and the installation opening form a first installation channel, a first installation channel is sealed by a third chip body, a plurality of groups of lower reaction units which are connected end to end are arranged on one upward side of the first chip body, the lower reaction units comprise a first lower reaction liquid flow channel, a second lower reaction liquid flow channel and a third lower reaction liquid flow channel which are sequentially connected together, a plurality of groups of upper reaction units which are connected end to end are arranged at one downward end of the second chip body, and the upper reaction units comprise a first upper reaction liquid flow channel, a second upper reaction liquid flow channel and a third lower reaction liquid flow channel which are sequentially connected together, a first reaction channel with closed upper and lower ends is formed between the first lower reaction liquid flow channel and the corresponding first upper reaction liquid flow channel, a second reaction channel with closed upper and lower ends is formed between the second lower reaction liquid flow channel and the corresponding second upper reaction liquid flow channel, and a third reaction channel with closed upper and lower ends is formed between the third lower reaction liquid flow channel and the corresponding third upper reaction liquid flow channel.

As a preferable embodiment of the chip capable of detecting a plurality of body fluids simultaneously according to the present invention, wherein: and a transmission motor is fixedly connected in the first mounting channel of the chip assembly and is connected with the rotating ring through a transmission belt.

The invention has the beneficial effects that: according to the invention, through the combined arrangement of the first reaction component and the chip component, the simultaneous detection of various body fluids is realized, and the detection efficiency is improved; through the arrangement of the second reaction component and the liquid inlet component, the detection of different proteins in saliva is realized.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise. Wherein:

fig. 1 is a front view of the present invention.

Fig. 2 is a first exploded view of the present invention.

Fig. 3 is a partially enlarged view of a portion a in fig. 2.

Fig. 4 is a first three-dimensional structure diagram of the present invention with the fifth chip body, the fourth chip body and the second reaction assembly hidden.

Fig. 5 is a perspective view of the center injection seat of the present invention.

Fig. 6 is a perspective view of the dispenser according to the present invention.

Fig. 7 is a perspective view of the liquid inlet module of the present invention.

Fig. 8 is a partial enlarged view of fig. 7 at B.

Fig. 9 is a first three-dimensional structure diagram of the fifth chip body of the invention after being hidden.

Fig. 10 is a partial enlarged view at C in fig. 9.

FIG. 11 is a perspective view showing the reaction ring of the present invention closely attached to the dispersion injection seat.

FIG. 12 is a structural view of a reaction ring in the present invention.

Fig. 13 is a view from the direction H-H in fig. 12.

Fig. 14 is an exploded view of the second embodiment of the present invention.

Fig. 15 is a partial enlarged view at D in fig. 14.

Fig. 16 is a partial enlarged view at E in fig. 14.

Fig. 17 is a second perspective view of the fifth chip body of the present invention with the fifth chip body hidden.

Fig. 18 is a partial enlarged view at F in fig. 17.

Fig. 19 is a perspective view of the first reaction module connected to the first chip body according to the present invention.

Fig. 20 is a partial enlarged view at G in fig. 19.

Fig. 21 is a perspective view of a fifth chip body according to the present invention.

In the figure, 100 chip components, 101a fifth chip body, 101a reaction liquid inlet hole, 101b liquid outlet part, 101c first upper liquid outlet channel, 102a fourth chip body, 102a first vertical through hole, 102b first lower liquid outlet channel, 102c lower waste liquid channel, 103 third chip body, 103a second vertical through hole, 103b first detection sink, 104 second chip body, 104a third vertical through hole, 104b upper reaction unit, 104b-1 second upper reaction liquid channel, 104b-2 first upper reaction liquid channel, 104b-3 third upper reaction liquid channel, 104c mounting port, 104d upper waste liquid channel, 105 first chip body, 105a reaction liquid inlet sink, 105b lower reaction unit, 105b-1 third lower reaction liquid channel, 105b-2 second lower reaction liquid channel, 105b-3 first lower reaction liquid channel, 105b-4 reaction liquid drain hole, 105b-5 reaction pool, 105b-6 detection chamber, 105c lifting hole, 105d fourth liquid inlet channel, 105e second liquid inlet channel, 105f first liquid inlet channel, 105g third liquid inlet channel, 105h installation sinking groove, 106 first detection groove, 107 installation groove, 107a fourth connection port, 107b third connection port, 107c second connection port, 107d first connection port, 107e connection sinking groove, 200 injection needle, 300 liquid inlet component, 301 fourth liquid inlet component, 301a liquid inlet housing, 301a-1 liquid outlet hole, 301a-2 liquid discharge part, 301b fixing ring, 301c middle shaft, 301c-1 liquid inlet, 301d linear driver, 301e guide rod, 301f guide sleeve, 301g fixing outer ring, 302 third liquid inlet component, 303 second liquid inlet component, 304 first liquid inlet component, 400 first reaction component, 401 collective injection seat, 401a collective injection port, 401b first effluent tank, 401c second effluent tank, 401d third effluent tank, 401e fourth effluent tank, 402 dispersed injection seat, 402a first effluent tank, 402b first effluent port, 402c second effluent port, 402d first effluent tank, 402e third effluent port, 402f second effluent tank, 402g third effluent port, 402h third effluent tank, 403 reaction ring, 403a injection chamber, 403b closed storage tank port, 403c reaction liquid storage tank, 404 guide shaft, 405 drive motor, 406 rotation ring, 407 drive belt, 408 first installation channel, 409 first electromagnet, 410 injection tube, 410a first effluent injection port, 410b second effluent injection port, 410c closed portion, 411 closed plug, 500 second reaction module, 501 reaction liquid control motor, 502 first effluent pump, 503 injection tube, 504 second storage shell, 504a second effluent inlet portion, 505 second metal sheet, 505a second limit part, 506 first storage shell, 506a first liquid inlet part, 507 first metal sheet, 507a first limit part, 508 second electromagnet, 509 mounting seat, 510 second liquid pump and 600 second detection groove.

Detailed Description

Before the technical solution of the present invention is explained, the terms used herein are defined as follows:

in order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.

Example 1

Referring to fig. 1 to 13, this embodiment provides a chip capable of detecting multiple body fluids simultaneously, which can detect multiple body fluids simultaneously and has high detection efficiency, as a first embodiment of the present invention.

A chip capable of detecting a plurality of body fluids simultaneously comprises a chip assembly 100, wherein one end of the chip assembly 100 is provided with a first detection groove 106, a first installation channel 408 is arranged in the chip assembly 100, a plurality of liquid inlet channels are distributed on the chip assembly 100 at one end of the first installation channel 408, a plurality of installation grooves 107 are distributed on the chip assembly 100, the chip assembly 100 is connected with the liquid inlet assemblies 300 through the installation grooves 107, a first reaction assembly 400 used for detecting the acidity and alkalinity of various body fluids is connected in the chip assembly 100, the first reaction assembly 400 comprises a concentrated injection seat 401 fixed in the chip assembly 100 and installed at one end of the first installation channel 408, a plurality of concentrated injection ports 401a communicated with the liquid inlet channels are distributed at one end of the concentrated injection seat 401, the other end of the concentrated injection seat 401 is connected with a dispersed injection seat 402, and one end of the dispersed injection seat 402, opposite to the concentrated injection seat 401, is provided with first liquid outlet grooves 401b, a plurality of liquid outlet grooves are independent from each other, A second liquid outlet 401c, a third liquid outlet 401d and a fourth liquid outlet 401e, wherein a first liquid inlet 402a corresponding to the first liquid outlet 401b, a second liquid inlet 402d corresponding to the second liquid outlet 401c, a third liquid inlet 402f corresponding to the third liquid outlet 401d and a fourth liquid inlet 402h corresponding to the fourth liquid outlet 401e are opened at one end of the dispersive injection seat 402 opposite to the collective injection seat 401, a plurality of first liquid outlets 402b communicating with the first liquid outlet 401b, a second liquid outlet 402c communicating with the second liquid outlet 401c, a third liquid outlet 402e communicating with the third liquid outlet 401d and a fourth liquid outlet 402g communicating with the fourth liquid outlet 401e are arranged at one end of the dispersive injection seat 402 far from the collective injection seat 401, a rotary ring 406 is rotatably connected in the chip assembly 100 at the other end of the first mounting channel 408, a transmission motor 405 is fixedly connected in the first mounting channel 408 of the chip assembly 100, the transmission motor 405 is connected with a rotary ring 406 through a transmission belt 407, one end of the dispersed injection seat 402 far away from the concentrated injection seat 401 is fixedly connected with a guide shaft 404, one end of the guide shaft 404 far away from the dispersed injection seat 402 is fixedly connected in the chip assembly 100, the rotary ring 406 is rotatably connected on the guide shaft 404, a reaction ring 403 controlled by magnetic force is slidably connected on the guide shaft 404, a plurality of injection pipes 410 controlled by magnetic force and corresponding to the first liquid outlets 402b, the second liquid outlets 402c, the third liquid outlets 402e and the fourth liquid outlets 402g are arranged on the reaction ring 403, a first injection inlet 410a and a second injection inlet 410b are arranged at an axial interval on the side of the injection pipe 410, a first electromagnet 409 is connected on the chip assembly 100 at the first detection groove 106, a plurality of reaction liquid storage pools 403c which are in one-to-one correspondence with the injection pipes 410 are arranged in the reaction ring 403, a plurality of sealing ports which are in one-to-one correspondence with the reaction liquid storage pools 403c are arranged on one side of the reaction ring 403 opposite to the rotating ring 406, a sealing plug 411 is connected with the sealing ports through the sealing ports, after the injection is finished, the sealing plug 411 is connected with the reaction ring 403 through the sealing ports, the liquid feeding of the reaction liquid is facilitated, the magnetism of the reaction ring 403 is smaller than that of the injection pipes 410, the reaction ring 403 can be inserted into the rotating ring 406, a sealing port 403b is arranged on the reaction ring 403 on one side of the reaction liquid storage pool 403c opposite to the injection pipes 410, an injection cavity 403a is formed between the sealing port 403b and the inner wall of one end of the reaction ring 403 which is far away from the reaction liquid storage pools 403c, a sealing part 410c is arranged at one end of the injection pipes 410 which is far away from the dispersive injection seat 402, and the sealing part 410c can just block the sealing port 403b, when the first inlet 410a of the inlet tube 410 is in the corresponding first outlet 402b, the second inlet 410b is in the injection cavity 403 a.

Further, the liquid inlet component 300 comprises a liquid inlet casing 301a which is connected in the corresponding mounting sinking groove 105h in a liftable manner and is provided with an upward opening, a lifting hole 105c is formed in the chip component 100 on the lower side of the liquid inlet component 300, a liquid discharging part 301a-2 is arranged on the outer side of the lower part of the liquid inlet casing 301a, a liquid outlet hole 301a-1 is formed in the liquid discharging part 301a-2, a pressing plate is connected in the liquid inlet casing 301a in a slidable manner, a center shaft 301c is fixedly connected to the center of the liquid inlet casing 301a, a fixing ring 301b is fixedly connected to one end, extending upwards, of the center shaft 301c out of the liquid inlet casing 301a, a fixing outer ring 301g is fixedly connected to the center shaft 301c below the fixing ring 301b, the periphery of the fixing outer ring 301g is attached to the inner ring of the compression plate, a malleable film is arranged between the fixing outer ring 301g and the pressing plate, the malleable film is a prior art, which is not shown, a tpu waterproof film can be selected, and in an initial state, the fixed outer ring 301g is flush with the upper side of the pressing plate, the film is in a folded state, at least two linear drivers 301d are distributed on the fixed ring 301b, a plurality of guide sleeves 301f are arranged on the fixed ring 301b between the linear drivers 301d, guide rods 301e are connected to the guide sleeves 301f in a sliding mode, the lower sides of the guide rods 301e are connected to the upper side of the pressing plate, telescopic rods 301h capable of moving in a reciprocating linear mode in the height direction are connected to the linear drivers 301d, the lower end of each telescopic rod 301h is connected to the upper side of the pressing plate, upward liquid inlet counter bores are formed in the middle shaft 301c, liquid inlets 301c-1 are formed in the side portions of the middle shaft 301c below the pressing plate, liquid inlet cavities are formed between the lower side of the pressing plate and the inner wall of the liquid inlet shell 301a, and the liquid inlet cavities are communicated with liquid outlet holes 301 a-1.

When liquid is fed, the injection needle 200 is inserted into the middle shaft 301c, body fluid is injected into the middle shaft 301c through the injection needle 200, enters the liquid feeding cavity through the liquid feeding hole 301c-1 in the middle shaft 301c, the pressing plate is moved downwards, the film is unfolded, the pressing plate and the fixed outer ring 301g are always in a sealed state, and the pressing plate moves downwards to promote the body fluid in the liquid feeding cavity to be discharged through the liquid discharging hole 301 a-1; when the body fluid is not discharged by moving down the pressing plate, the body fluid can be discharged through the liquid outlet hole 301a-1 by connecting the peristaltic pump to the upper end of the injection needle 200.

In this embodiment, there are four liquid inlet channels, which are a first liquid inlet channel 105f, a second liquid inlet channel 105e, a third liquid inlet channel 105g and a fourth liquid inlet channel 105d, the liquid inlet assemblies 300 are four groups, the four liquid inlet assemblies 300 are a first liquid inlet assembly 304, a second liquid inlet assembly 303, a third liquid inlet assembly 302 and a fourth liquid inlet assembly 301, the first liquid inlet assembly 304 outputs urine to the first liquid inlet channel 105f, the second liquid inlet assembly 303 outputs sweat to the second liquid inlet channel 105e, the third liquid inlet assembly 302 outputs tear to the third liquid inlet channel 105g, the fourth liquid inlet assembly 301 outputs saliva to the fourth liquid inlet channel 105d, the urine enters the injection tube 410 through the first liquid inlet channel 105f, the corresponding concentration injection port 401a, the first liquid outlet channel 401b, the first liquid inlet channel 402a, the first liquid outlet channel 402b and the corresponding first inflow injection port 410a in sequence.

The reaction ring 403, the reaction ring 403 and the section of the chip assembly 100 between the detection grooves are all transparent; before detection, a plurality of lifting structures matched with the liquid inlet component 300 are arranged on the lower side of the chip, the lifting structures are the prior art, specific structures of the lifting structures are not shown, the lifting structures can be lifting drivers, lifting rods which extend upwards and can do reciprocating linear movement are connected to the lifting drivers, the lifting rods can just penetrate through the lifting holes 105c to be contacted with the bottom side of the liquid inlet shell 301a, when the liquid inlet shell 301a abuts against the bottom of the chip component 100, liquid outlet holes 301a-1 of the first liquid inlet component 304 are communicated with the first liquid inlet channel 105f, liquid outlet holes 301a-1 of the second liquid inlet component 303 are communicated with the second liquid inlet channel 105e, liquid outlet holes 301a-1 of the third liquid inlet component 302 are communicated with the third liquid inlet channel 105g, and liquid outlet holes 301a-1 of the fourth liquid inlet component 301 are communicated with the fourth liquid inlet channel 105 d; in an initial state, the reaction ring 403 is tightly attached to one side of the dispersion injection seat 402 far from the concentration injection seat 401, four liquid inlet channels are provided, different body fluids are introduced into the corresponding liquid inlet channels through the liquid inlet assemblies 300, the different body fluids enter the concentration injection ports 401a through the corresponding liquid inlet channels, the body fluids flowing out of the four concentration injection ports 401a enter the first liquid outlet groove 401b, the second liquid outlet groove 401c, the third liquid outlet groove 401d and the fourth liquid outlet groove 401e, the body fluids in the first liquid outlet groove 401b are divided into a plurality of paths and output from the first liquid outlet holes 402b respectively, the body fluids enter the injection pipe 410 through the corresponding first flow-in injection ports 410a, the body fluids in the second liquid outlet groove 401c enter the corresponding injection pipes 410 through the second liquid outlet holes 402c and the corresponding first flow-in injection ports 410a in sequence, and the body fluids in the third liquid outlet groove 401d enter the corresponding injection pipes 410 through the third liquid outlet holes 402e and the corresponding first flow-in sequences In 410, the body fluid in the fourth liquid outlet tank 401e enters the corresponding injection pipe 410 through the fourth liquid outlet 402g and the corresponding first inflow injection inlet 410a in sequence, the liquid inlet is finished, the first electromagnet 409 is electrified, the strength of the first electromagnet 409 is adjusted, the injection pipe 410 is sucked into the corresponding reaction liquid storage tank 403c, the reaction ring 403 is not moved, the outer end of the injection pipe 410 just seals the reaction ring 403, the body fluid reacts with the reaction liquid in the reaction liquid storage tank 403c, the strength of the first electromagnet 409 is increased, the reaction ring 403 slides towards the direction of the rotating ring 406 along the guide shaft 404 until the reaction ring 403 slides onto the rotating ring 406, the transmission motor 405 acts, the rotating ring 406 is driven to rotate by the transmission belt 407, after the body fluid and the reaction liquid are fully mixed, the reaction liquid storage tank 403c is aligned with the color sensor in sequence, the color sensor sequentially recognizes the color change after the reaction, for analysis; according to the invention, through the combined arrangement of the first reaction component 400 and the chip component 100, the simultaneous detection of various body fluids is realized, and the detection efficiency is improved.

Example 2

Referring to fig. 14 to 21, a second embodiment of the present invention is different from the second embodiment in that the present embodiment provides a chip capable of simultaneously detecting a plurality of body fluids, and the present embodiment can realize detection of a plurality of proteins in saliva.

A chip capable of detecting a plurality of body fluids simultaneously comprises a second reaction assembly 500, wherein the second reaction assembly 500 is arranged on a chip assembly 100 between a fourth liquid inlet assembly 301 and a third liquid inlet assembly 302, a second installation channel is further arranged in the chip assembly 100, the second reaction assembly 500 comprises a first storage shell 506 and a plurality of second storage shells 504 which are connected in the second installation channel, a vertically arranged reaction liquid outlet flow channel is arranged in the chip assembly 100, an inner cavity of the first storage shell 506 can be communicated with the reaction liquid outlet flow channel, an inner cavity of the second storage shell 504 can be communicated with the reaction liquid outlet flow channel, an inner cavity of the first storage shell 506 can be communicated with a liquid inlet cavity in one of the liquid inlet assemblies 300, a plurality of groups of reaction units which are connected end to end are further arranged in the chip assembly 100, each reaction unit comprises a first reaction channel, one end of each first reaction channel in each reaction unit at the head end is communicated with the reaction liquid outlet flow channel, the other end of the first reaction channel is connected with one end of the second reaction channel, the other end of the second reaction channel is communicated with one end of the third reaction channel, and the other end of the previous third reaction channel in two adjacent reaction units is communicated with one end of the next first reaction channel.

In order to further realize the simultaneous detection of a plurality of proteins in saliva, a reaction liquid control motor 501 is fixedly connected in the chip assembly 100, a liquid injection pipe 503 extending downwards is connected on the reaction liquid control motor 501, a first liquid inlet part 506a is arranged on one side of the first storage shell 506, which is arranged opposite to the liquid injection pipe 503, a second liquid inlet part 504a is arranged on one side of the second storage shell 504, which is arranged opposite to the liquid injection pipe 503, one end of the liquid injection pipe 503 can be attached to the outer side of the first liquid inlet part 506a and aligned with the first liquid inlet part 506a or attached to the outer side of the second liquid inlet part 504a and aligned with the second liquid inlet part 504a, a first liquid pump 502 is fixedly connected to the other end of the liquid injection pipe 503, the lower end of the liquid injection pipe 503 is connected with the upper end of a reaction liquid flow passage, the first reaction passage and the second reaction passage are arranged in parallel, a plurality of reaction cells 105b-5 are arranged in the chip assembly 100 between the first reaction passage and the second reaction passage, the chip assembly 100 at one side of the first reaction channel and the third reaction channel, which faces the reaction tank 105b-5, is provided with a reaction liquid drainage hole 105b-4, the chip assembly 100 at the other end of the third reaction channel in the terminal reaction unit is provided with a waste liquid drainage channel, a waste liquid tank is arranged in the chip assembly 100, one end of the waste liquid drainage channel, which is far away from the terminal third reaction channel, is connected with the waste liquid tank, and the chip assembly 100 at one end of the waste liquid tank, which is far away from the reaction unit, is connected with a second liquid suction pump 510.

Further, the chip assembly 100 includes a first chip body 105, a second chip body 104, a third chip body 103, a fourth chip body 102, and a fifth chip body 101, which are sequentially connected together from bottom to top, and one end of the guide shaft 404, which is far away from the dispersed injection seat 402, is fixedly connected in the first chip body 105; four connecting sunken grooves 107e which are arranged in a rectangular shape are arranged on the upward side of the first chip body 105, four first connecting ports 107d which are arranged in a rectangular shape are arranged on the second chip body 104, four second connecting ports 107c which are arranged in a rectangular shape are arranged on the third chip body 103, four third connecting ports 107b which are arranged in a rectangular shape are arranged on the fourth chip body 102, four fourth connecting ports 107a which are arranged in a rectangular shape are arranged on the fifth chip body 101, and the fourth connecting ports 107a, the third connecting ports 107b, the second connecting ports 107c, the first connecting ports 107d and the corresponding connecting sunken grooves 107e form mounting grooves 107; the first detection groove 106 is provided at the side ends of the first chip body 105, the second chip body 104, and the third chip body 103; a lower waste liquid channel 102c is arranged on the upward side of the first chip body 105, an upper waste liquid channel 104d corresponding to the lower waste liquid channel 102c is arranged on the downward side of the second chip body 104, and the lower waste liquid channel 102c and the upper waste liquid channel 104d form a waste liquid pool; the fourth chip body 102 is provided with a first vertical through hole 102a aligned with the vertical section of the liquid injection pipe 503, the third chip body 103 is provided with a second vertical through hole 103a coaxial with the first vertical through hole 102a, the second chip body 104 is provided with a third vertical through hole 104a coaxial with the second vertical through hole 103a, the upward side of the first chip body 105 is provided with a reaction liquid inlet sink groove 105a, the first vertical through hole 102a, the second vertical through hole 103a, the third vertical through hole 104a and the reaction liquid inlet sink groove 105a form a reaction liquid outlet flow channel, the upward side of the first chip body 105 is provided with an installation sink groove 105h, the second chip body 104 is provided with an installation opening 104c corresponding to the installation sink groove 105h, the installation sink groove 105h and the installation opening 104c form a first installation channel 408, the third chip body 103 seals the first installation channel 408, a plurality of groups of lower reaction units 105b connected end to end are arranged on the upward side of the first chip body 105, the lower reaction unit 105b comprises two groups of first lower reaction liquid flow channels 105b-3, second lower reaction liquid flow channels 105b-2 and third lower reaction liquid flow channels 105b-1 which are sequentially connected together, a plurality of groups of upper reaction units 104b which are connected end to end are arranged at the downward end of the second chip body 104, each upper reaction unit 104b comprises two groups of first upper reaction liquid flow channels 104b-2, second upper reaction liquid flow channels 104b-1 and third lower reaction liquid flow channels 105b-1 which are sequentially connected together, first reaction channels with closed upper and lower ends are formed between the first lower reaction liquid flow channels 105b-3 and the corresponding first upper reaction liquid flow channels 104b-2, second reaction channels with closed upper and lower ends are formed between the second lower reaction liquid flow channels 105b-2 and the corresponding second upper reaction liquid flow channels 104b-1, and third lower reaction liquid flow channels 105b-1 and the corresponding third upper reaction liquid flow channels 104b-3 are formed between the corresponding lower reaction liquid flow channels 105b-2 and the corresponding upper reaction liquid flow channels A third reaction channel with closed upper and lower ends is formed, a detection chamber 105b-6 is formed at the upward side of the first chip body 105 and the downward side of the second chip body 104 between the first reaction channel and the third reaction channel, a reaction liquid discharge hole 105b-4 is opened on the first chip body 105 at the side of the first reaction channel and the third reaction channel facing the detection chamber 105b-6, the reaction cells 105b-5 are all arranged on the first chip body 105 corresponding to the position of the detection chamber 105b-6, different antibodies are coated in the reaction cells 105b-5 in different reaction units, a connection groove is opened at the downward end of the fifth chip body 101, a reaction liquid control motor 501 is fixedly connected in the fifth chip body 101 through the connection groove, a liquid injection pipe 503 is rotatably connected in the fourth chip body 102, a first upper liquid outlet flow passage 101c is arranged at the downward side of the fifth chip body 101 close to the first storage shell 506, a first lower liquid outlet flow channel 102b corresponding to the first upper liquid outlet flow channel 101c is arranged on the upward side of the fourth chip body 102, a first liquid outlet channel is formed by the first upper liquid outlet flow channel 101c and the first lower liquid outlet flow channel 102b, one end of the first liquid outlet channel can be aligned with a liquid outlet hole 301a-1 of a liquid inlet shell 301a in the fourth liquid inlet assembly 301, one end of a first liquid inlet channel 105f, a second liquid inlet channel 105e, a third liquid inlet channel 105g and one end of a fourth liquid inlet channel 105d are respectively communicated with the corresponding liquid outlet hole 301a-1 on the liquid inlet shell 301a, a first lower liquid inlet flow channel, a second lower liquid inlet flow channel, a third lower liquid inlet flow channel and a fourth lower liquid inlet flow channel are arranged on the upward side of the first chip body 105, a first upper liquid inlet flow channel corresponding to the first lower liquid inlet flow channel, a second upper liquid inlet flow channel corresponding to the second lower liquid inlet flow channel, a liquid inlet flow channel corresponding to the second lower liquid inlet flow channel, A third upper liquid inlet channel corresponding to the third lower liquid inlet channel and a fourth upper liquid inlet channel corresponding to the fourth lower liquid inlet channel, wherein the first lower liquid inlet channel and the first upper liquid inlet channel form a first liquid inlet channel 105f, the second lower liquid inlet channel and the second upper liquid inlet channel form a second liquid inlet channel 105e, the third lower liquid inlet channel and the first third liquid inlet channel form a third liquid inlet channel 105g, the fourth lower liquid inlet channel and the fourth upper liquid inlet channel form a fourth liquid inlet channel 105d, the other end of the first liquid outlet channel is connected with one end of the first storage shell 506 far away from the first liquid inlet part 506a, a mounting seat 509 is slidably connected in the fourth chip body 102, the mounting seat 509 is fixedly arranged on the downward side of one end of the liquid inlet pipe 503 far away from the first liquid pump 502, a second electromagnet 508 is fixedly connected in the mounting seat 509, one end of the first storage shell 506 close to the first liquid inlet part 506a can be lifted and connected with a first metal sheet 507, a first limiting portion 507a is arranged at the upper portion of the first metal sheet 507, the first limiting portion 507a abuts against the upper side of the first storage shell 506 in an initial state, the first metal sheet 507 separates an inner cavity of the first storage shell 506 from the first liquid inlet portion 506a, a second metal sheet 505 is connected to one end, close to the second liquid inlet portion 504a, of the second storage shell 504 in a lifting mode, a second limiting portion 505a is arranged at the upper portion of the second metal sheet 505, the second limiting portion 505a abuts against the upper side of the second storage shell 504 in the initial state, the inner cavity of the second storage shell 504 is separated from the second liquid inlet portion 504a by the second metal sheet 505, and the second electromagnet 508 can be aligned with the first metal sheet 507 or the second metal sheet 505.

In an initial state, the liquid injection pipe 503 enables the second liquid inlet assembly 303 to ascend through an external lifting driver, when the liquid outlet hole 301a-1 moves upwards to one end aligned with the first liquid outlet channel, the lifting driver stops acting, saliva is introduced into the liquid inlet cavity through the injection needle 200, and the saliva sequentially enters the first storage shell 506 through the liquid outlet hole 301a-1 and the first liquid outlet channel; the side part of the third chip body 103 and the side part of the fourth chip body 102 are respectively provided with a first detection sinking groove 103b and a second detection sinking groove, the first detection sinking groove 103b and the second detection sinking groove form a second detection groove 600, the second detection groove 600 covers the area where each reaction unit is located, in the embodiment, four groups of reaction units are arranged, and each group of reaction units realizes the detection of different proteins in saliva; in order to facilitate liquid feeding in the second storage shell 504, a reaction liquid inlet hole 101a is formed in one upward side of the second storage shell 504, a plurality of injection holes corresponding to the reaction liquid inlet holes 101a one to one are distributed in the fifth chip body 101, liquid injection parts 101b corresponding to the injection holes one to one are distributed in the lower side of the fifth chip body 101, and the liquid injection parts 101b are inserted into the reaction liquid inlet holes 101a in the corresponding second storage shell 504.

Taking the detection of transferrin in saliva as an example, the detection method comprises the following steps:

(1) controlling the second electromagnet 508 to move, enabling the first metal sheet 507 to move upwards, enabling the first liquid inlet part 506a to be communicated with the liquid injection pipe 503, enabling the first liquid pump 502 to move, sucking saliva in the first storage shell 506, enabling the saliva to sequentially enter the first reaction channel, the second reaction channel and the third reaction channel through the reaction liquid outlet flow channel, enabling the saliva to enter the reaction pool 105b-5 of the detection chamber 105b-6 through the reaction liquid outlet hole 105b-4, stopping the saliva liquid discharge, closing the second electromagnet 508, enabling the first metal sheet 507 to move downwards, and separating the first liquid inlet part 506a from the inner cavity of the first storage shell 506 again;

(2) the reaction liquid control motor 501 acts, the liquid injection pipe 503 rotates, the liquid injection pipe 503 is made to rotate to the position where the next second liquid inlet part 504a is located, the reaction liquid control motor 501 stops acting, the second electromagnet 508 is started, the second metal sheet 505 moves upwards and is opened, the first liquid pump 502 is started, the detergent in the second storage shell 504 is sucked, the detergent flows into the reaction tank 105b-5, the unbound sample is removed, the second electromagnet 508 is closed, the first liquid pump 502 is closed, the second liquid pump 510 is opened, and the redundant waste liquid is pumped into the waste liquid tank;

(3) the reaction liquid control motor 501 acts to make the liquid injection pipe 503 aim at the next second liquid inlet part 504a, the reaction liquid control motor 501 stops acting, the second electromagnet 508 is started, the second metal sheet 505 moves upwards and is opened, the first liquid pump 502 is started to suck the biotin-modified antibody in the second storage shell 504 containing the biotin modification, the biotin-modified antibody enters the transferrin detection chamber 105b-6 at the head end for incubation, the incubation is finished, the second electromagnet 508 and the first liquid pump 502 are closed, the second metal sheet 505 moves downwards, the second liquid pump 510 is opened, and the redundant waste liquid is pumped into the waste liquid pool;

(4) the reaction liquid control motor 501 acts, the liquid injection pipe 503 rotates to a position where the next second liquid inlet part 504a is located, the reaction liquid control motor 501 stops acting, the second electromagnet 508 starts, the second metal sheet 505 moves upwards and opens, the first liquid suction pump 502 starts to suck the detergent in the second storage shell 504, the detergent flows into the reaction tank 105b-5 to remove the redundant biotin antibody, the second electromagnet 508 is closed, the first liquid suction pump 502 is closed, the second liquid suction pump 510 is opened, and the redundant waste liquid is sucked into the waste liquid tank;

(5) the reaction liquid control motor 501 acts, the liquid injection pipe 503 rotates to the position where the next second liquid inlet part 504a is located, the reaction liquid control motor 501 stops acting, the second electromagnet 508 starts, the second metal sheet 505 moves upwards and opens, the first liquid pump 502 starts to suck the horseradish peroxidase reaction liquid containing the avidin marks in the second storage shell 504, the horseradish peroxidase reaction liquid containing the avidin marks flows into the reaction tank 105b-5, the reaction is finished, the second electromagnet 508 is closed, the first liquid pump 502 is closed, the second liquid pump 510 is opened, and the redundant waste liquid is pumped into the waste liquid tank;

(6) the reaction liquid control motor 501 acts, the liquid injection pipe 503 rotates, the liquid injection pipe 503 is made to rotate to the position where the next second liquid inlet part 504a is located, the reaction liquid control motor 501 stops acting, the second electromagnet 508 is started, the second metal sheet 505 moves upwards and is opened, the first liquid suction pump 502 is started, reaction liquid containing tetramethyl benzidine substrates in the second storage shell 504 is sucked and flows into the reaction tank 105b-5, color development reaction occurs, the reaction is finished, the second electromagnet 508 is closed, the first liquid suction pump 502 is closed, the second liquid suction pump 510 is opened, and redundant waste liquid is sucked into the waste liquid tank;

(7) the reaction liquid control motor 501 acts, the liquid injection pipe 503 rotates, the liquid injection pipe 503 is made to rotate to the position of the second liquid inlet portion 504a aligned with the tail end, the reaction liquid control motor 501 stops acting, the second electromagnet 508 starts, the second metal sheet 505 moves upwards and is opened, the first liquid pump 502 starts to suck the stop liquid in the second storage shell 504, the stop liquid flows into the reaction tank 105b-5, the color reaction is stopped, the reaction is finished, the second electromagnet 508 is closed, the first liquid pump 502 is closed, the second liquid pump 510 is opened, and the redundant waste liquid is pumped into the waste liquid tank;

(8) the light source facing the second detection groove 600 is placed on the lower side of the first chip body 105, the colorimetric camera is inserted into the second detection groove 600, the colorimetric camera shoots the color development condition, and the detection of the transferrin in the saliva is realized.

According to the invention, through the arrangement of the second reaction component 500 and the liquid inlet component 300, the detection of different proteins in saliva is realized.

It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.

Claims

1. A chip capable of detecting a plurality of body fluids simultaneously is characterized in that: which comprises

The chip assembly (100), one end of the chip assembly (100) is provided with a first detection groove (106), a first installation channel (408) is arranged in the chip assembly (100), a plurality of liquid inlet channels are distributed on the chip assembly (100) at one end of the first installation channel (408), and a plurality of installation grooves (107) are distributed on the chip assembly (100);

the liquid inlet components (300) are provided with a plurality of groups and correspond to the mounting grooves (107) one by one, and the liquid inlet components (300) are mounted in the corresponding mounting grooves (107);

first reaction assembly (400), first reaction assembly (400) including fix in chip subassembly (100) and install in the concentrated injection seat (401) of first installation passageway (408) one end, the concentrated injection mouth (401 a) of a plurality of and inlet channel intercommunication have been arranged to the one end of concentrated injection seat (401), the other end of concentrated injection seat (401) is connected with the dispersion and injects seat (402), the one end of dispersed injection seat (402) relative concentrated injection seat (401) is opened has first drain groove (401 b) that mutually independent, second drain groove (401 c), third drain groove (401 d) and fourth drain groove (401 e), and the one end that dispersed injection seat (402) kept away from concentrated injection seat (401) has arranged a plurality of first liquid outlets (402 b) that communicate with first drain groove (401 b), second liquid outlets (402 c) that communicate with second drain groove (401 c), A third liquid outlet (402 e) communicated with a third liquid outlet groove (401 d) and a fourth liquid outlet (402 g) communicated with the fourth liquid outlet groove (401 e), a rotary ring (406) is rotatably connected in the chip assembly (100) at the other end of the first mounting channel (408), one end of the dispersed injection seat (402) far away from the concentrated injection seat (401) is fixedly connected with a guide shaft (404), one end of the guide shaft (404) far away from the dispersed injection seat (402) is fixedly connected in the chip assembly (100), the guide shaft (404) is slidably connected with a reaction ring (403) which is controlled by magnetic force and can be inserted into the rotary ring (406), a plurality of injection pipes (410) which are in one-to-one correspondence with the liquid outlets and controlled by magnetic force are distributed on the reaction ring (403), and a first injection inlet (410 a) and a second injection inlet (410 b) which are axially arranged at intervals are arranged on the side of the injection pipes (410), the chip assembly (100) at the first detection groove (106) is connected with a first electromagnet (409), a plurality of reaction liquid storage pools (403 c) which are in one-to-one correspondence with the injection pipes (410) are arranged in the reaction ring (403), and the magnetism of the reaction ring (403) is smaller than that of the injection pipes (410).

2. The chip of claim 1, wherein the chip is capable of simultaneously detecting a plurality of body fluids: the reaction ring (403) on one side of the reaction liquid storage tank (403 c) opposite to the injection pipe (410) is provided with a closed opening (403 b), an injection cavity (403 a) is formed between the closed opening (403 b) and the inner wall of one end, far away from the reaction liquid storage tank (403 c), of the reaction ring (403), one end, far away from the dispersed injection seat (402), of the injection pipe (410) is provided with a closed part (410 c), the closed part (410 c) can just block the closed opening (403 b), and when a first inflow injection opening (410 a) of the injection pipe (410) is in the corresponding first liquid outlet (402 b), a second inflow injection opening (410 b) is in the injection cavity (403 a).

3. The chip of claim 1, wherein the chip is capable of simultaneously detecting a plurality of body fluids: the feed liquor subassembly (300) is connected in corresponding installation heavy groove (105 h) and has feed liquor shell (301 a) of opening up including liftable ground, be connected with the clamp plate in feed liquor shell (301 a) slidable, center fixedly connected with axis (301 c) of feed liquor shell (301 a), one end fixedly connected with solid fixed ring (301 b) that axis (301 c) upwards stretched out feed liquor shell (301 a), fixed ring (301 b) are arranged and are had two at least sharp driver (301 d), be connected with on sharp driver (301 d) can be in the direction of height reciprocal linear motion's telescopic link (301 h), the lower extreme of telescopic link (301 h) is connected at the clamp plate upside, it has up feed liquor to open on axis (301 c), axial (301 c) lateral part below the clamp plate is opened has inlet (301 c-1), a liquid inlet cavity is formed between the lower side of the pressure plate and the inner wall of the liquid inlet shell (301 a).

4. A chip capable of simultaneously detecting a plurality of body fluids according to any one of claims 1 to 3, wherein: still include second reaction assembly (500), still be equipped with second installation passageway in chip subassembly (100), second reaction assembly (500) are including connecting first storage shell (506) and a plurality of second storage shell (504) in second installation passageway, be equipped with the reaction play liquid runner of vertical setting in chip subassembly (100), the inner chamber of first storage shell (506) can communicate with reaction play liquid runner, the inner chamber of second storage shell (504) can communicate with reaction play liquid runner, the inner chamber of first storage shell (506) can communicate with the feed liquor chamber in one of them feed liquor subassembly (300), still be equipped with a plurality of groups of end to end's reaction unit in chip subassembly (100), the reaction unit includes first reaction channel, first reaction channel one end and reaction play liquid runner intercommunication in the reaction unit of head end, the one end of second reaction channel is connected to the first reaction channel other end, the other end of the second reaction channel is communicated with one end of a third reaction channel, and the other end of the previous third reaction channel in two adjacent reaction units is communicated with one end of the next first reaction channel.

5. The chip according to claim 4, wherein the chip is capable of simultaneously detecting a plurality of body fluids: fixedly connected with reaction liquid control motor (501) in chip subassembly (100), be connected with notes liquid pipe (503) that stretches out downwards on reaction liquid control motor (501), one side that first storage shell (506) set up relative notes liquid pipe (503) is equipped with first liquid portion (506 a) of advancing, one side that second storage shell (504) set up relative notes liquid pipe (503) is equipped with second liquid portion (504 a) of advancing, the one end of annotating liquid pipe (503) can be laminated in first liquid portion (506 a) outside and aim at first liquid portion (506 a) or laminated in second liquid portion (504 a) outside and aim at second liquid portion (504 a), the other end fixedly connected with first drawing liquid pump (502) of annotating liquid pipe (503), the lower extreme and the upper end of reaction liquid outlet flow channel of annotating liquid pipe (503) meet.

6. The chip according to claim 4, wherein the chip is capable of simultaneously detecting a plurality of body fluids: the first reaction channel and the second reaction channel are arranged in parallel, a plurality of reaction pools (105 b-5) are arranged in the chip assembly (100) between the first reaction channel and the second reaction channel, and reaction liquid drain holes (105 b-4) are formed in the chip assembly (100) on one side, facing the reaction pools (105 b-5), of the first reaction channel and the third reaction channel.

7. The chip according to claim 4, wherein the chip is capable of simultaneously detecting a plurality of body fluids: a waste liquid outlet channel is arranged in the chip assembly (100) at the other end of the third reaction channel in the tail end reaction unit, a waste liquid pool is arranged in the chip assembly (100), and one end, far away from the tail end of the third reaction channel, of the waste liquid outlet channel is connected with the waste liquid pool.

8. The chip of claim 7, wherein the chip is capable of simultaneously detecting a plurality of body fluids: and a second liquid pump (510) is connected in the chip assembly (100) at one end of the waste liquid pool, which is far away from the reaction unit.

9. The chip of claim 5, wherein the chip is capable of simultaneously detecting a plurality of body fluids: the chip assembly (100) comprises a first chip body (105) and a second chip body (104) which are sequentially connected together from bottom to top, wherein an installation sinking groove (105 h) is formed in one upward side of the first chip body (105), an installation opening (104 c) corresponding to the installation sinking groove (105 h) is formed in the second chip body (104), the installation sinking groove (105 h) and the installation opening (104 c) form a first installation channel (408), a first installation channel (408) is sealed by a third chip body (103), a plurality of groups of lower reaction units (105 b) which are connected end to end are arranged in one upward side of the first chip body (105), the lower reaction units (105 b) comprise a first lower reaction liquid flow channel (105 b-3), a second lower reaction liquid flow channel (105 b-2) and a third lower reaction liquid flow channel (105 b-1) which are sequentially connected together, a plurality of groups of upper reaction units (104 b) which are connected end to end are arranged at the downward end of the second chip body (104), the upper reaction unit (104 b) includes a first upper reaction liquid flow path (104 b-2), a second upper reaction liquid flow path (104 b-1), and a third lower reaction liquid flow path (105 b-1) connected in sequence, a first reaction channel with closed upper and lower ends is formed between the first lower reaction liquid flow channel (105 b-3) and the corresponding first upper reaction liquid flow channel (104 b-2), a second reaction channel with closed upper and lower ends is formed between the second lower reaction liquid flow channel (105 b-2) and the corresponding second upper reaction liquid flow channel (104 b-1), and a third reaction channel with closed upper and lower ends is formed between the third lower reaction liquid flow channel (105 b-1) and the corresponding third upper reaction liquid flow channel (104 b-3).

10. A chip capable of simultaneously detecting a plurality of body fluids according to any one of claims 1 to 3, wherein: a transmission motor (405) is fixedly connected in the first mounting channel (408) of the chip assembly (100), and the transmission motor (405) is connected with a rotating ring (406) through a transmission belt (407).